TGFA cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	TGFA
Entrez Gene ID	21802
Full Name	transforming growth factor alpha
Synonyms	wa-1,wa1
General protein information	Preferred Names transforming growth factor alpha Names protransforming growth factor alpha
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 6 Map Location: 6 D1\|6 37.62 cM
Summary	This gene encodes a member of the epidermal growth factor (EGF) family of proteins that regulate cellular proliferation. The encoded protein undergoes proteolytic processing to generate a soluble glycoprotein that is secreted by the cell. The secreted protein binds to the EGF receptors to initiate signaling events resulting in cellular proliferation, mucous production or inhibition of gastric acid secretion. The transgenic expression of the encoded protein in mice induces the development of cancers in various tissues such as liver, pancreas, skin and mammary glands. Mice lacking the encoded protein exhibit a wavy coat and curly whiskers phenotype as well as abnormalities in the eye. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_030255312.1	XP_030111172.1	protransforming growth factor alpha isoform X1
NM_031199.4	NP_112476.1	protransforming growth factor alpha preproprotein
NM_031199.3	NP_112476.1	protransforming growth factor alpha preproprotein

Pathways from BioSystems

Homologies

	TGFA	XP_002799317.1
Homo sapiens (human)	TGFA	NP_003227.1
Rattus norvegicus (Norway rat)	Tgfa	NP_036803.1
Bos taurus (cattle)	TGFA	XP_002691279.2
Mus musculus (house mouse)	Tgfa	NP_112476.1
Pan troglodytes (chimpanzee)	TGFA	XP_001142892.1
Canis lupus familiaris (dog)	TGFA	XP_005626313.1
Gallus gallus (chicken)	TGFA	NP_001001614.1
Xenopus tropicalis (tropical clawed frog)	LOC100485434	XP_002935348.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of TGF-alpha in the progression of diabetic kidney disease.
Heuer JG, Harlan SM, Yang DD, Jaqua DL, Boyles JS, Wilson JM, Heinz-Taheny KM, Sullivan JM, Wei T, Qian HR, Witcher DR, Breyer MD
American journal of physiology. Renal physiology312(6)F951-F962(2017 Jun)

Context-specific protection of TGF? null mice from osteoarthritis.
Usmani SE, Ulici V, Pest MA, Hill TL, Welch ID, Beier F
Scientific reports630434(2016 Jul)

Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGF?.
Wunderle L, Knopf JD, K?hnle N, Morl? A, Hehn B, Adrain C, Strisovsky K, Freeman M, Lemberg MK
Scientific reports627342(2016 Jun)

Mechanisms of Lung Fibrosis Resolution.
Glasser SW, Hagood JS, Wong S, Taype CA, Madala SK, Hardie WD
The American journal of pathology186(5)1066-77(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TGF-alpha contributes to the progression of diabetic kidney disease.
Title: Role of TGF-alpha in the progression of diabetic kidney disease.

Perturbed meibomian gland and tarsal plate morphogenesis by excess TGFalpha in eyelid stroma.
Title: Perturbed meibomian gland and tarsal plate morphogenesis by excess TGF? in eyelid stroma.

c-Myc and transforming growth factor alpha enhance the development of hepatic lesions due to mutant beta-catenin in transgenic mice.
Title: c-Myc and transforming growth factor ? enhance the development of hepatic lesions due to mutant ?-catenin in transgenic mice.

Reverse transcriptase polymerase chain reaction studies showed that three members, Tgfa, Hbegf,and Nrg1 of the EGF family were expressed in the epithelium cultured with FGF7 + LPA as well as in the epithelium freshly isolated from the rudiments.
Title: FGF7 signals are relayed to autocrine EGF family growth factors to induce branching morphogenesis of mouse salivary epithelium.

findings suggest that elevated PHD4 levels disturb the angiogenic balance in osteosarcoma via induction of the TGF-alpha pathway and inhibit tumor growth by reducing the expression of HIF-2alpha
Title: PHD4 stimulates tumor angiogenesis in osteosarcoma cells via TGF-?.

The following TGFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15684	NM_031199.4 Latest version!	Mus musculus transforming growth factor alpha (Tgfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu89177	XM_030255312.1 Latest version!	Mus musculus transforming growth factor alpha (Tgfa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu15684	NM_031199.3	Mus musculus transforming growth factor alpha (Tgfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGFA ( NM_031199.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_112476.1 Mus musculus transforming growth factor alpha (Tgfa), mRNA.

Price & Availability↑

CloneID	OMu15684
Clone ID Related Accession (Same CDS sequence)	NM_031199.4 , NM_031199.3
Accession Version	NM_031199.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	protransforming growth factor alpha preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK010785.1, AC159549.11, AK165017.1 and AK162680.1. On Sep 18, 2015 this sequence version replaced NM_031199.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK162680.1, U65016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTCCCCG CGACCGGACA GCTCGCTCTG CTAGCGCTGG GTATCCTGTT AGCTGTGTGC 
CAGGCTCTGG AGAACAGCAC ATCCCCCCTG AGTGACTCAC CCGTGGCGGC TGCAGTGGTG 
TCTCACTTCA ACAAGTGCCC AGATTCCCAC ACTCAGTACT GCTTCCATGG AACCTGCCGG 
TTTTTGGTGC AGGAAGAGAA GCCAGCATGT GTCTGCCACT CTGGGTACGT GGGTGTTCGC 
TGTGAGCATG CAGACCTCCT GGCTGTGGTG GCTGCCAGCC AGAAGAAGCA AGCCATCACT 
GCCCTGGTGG TGGTCTCCAT TGTGGCCCTG GCTGTCCTCA TTATCACCTG TGTGCTGATC 
CACTGCTGTC AGCTCCGCAA ACACTGTGAG TGGTGCCGTG CCCTCGTCTG CAGACATGAG 
AAGCCCAGCG CCCTCCTGAA GGGAAGGACT GCTTGCTGCC ACTCTGAGAC AGTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112476.1
CDS	280..759
Translation	MVPATGQLALLALGILLAVCQALENSTSPLSDSPVAAAVVSHFNKCPDSHTQYCFHGTCRFLVQEEKPACVCHSGYVGVRCEHADLLAVVAASQKKQAITALVVVSIVALAVLIITCVLIHCCQLRKHCEWCRALVCRHEKPSALLKGRTACCHSETVV

Target ORF information:

RefSeq Version	NM_031199.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus transforming growth factor alpha (Tgfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031199.4

ORF Insert Sequence:

ATGGTCCCCG CGACCGGACA GCTCGCTCTG CTAGCGCTGG GTATCCTGTT AGCTGTGTGC 
CAGGCTCTGG AGAACAGCAC ATCCCCCCTG AGTGACTCAC CCGTGGCGGC TGCAGTGGTG 
TCTCACTTCA ACAAGTGCCC AGATTCCCAC ACTCAGTACT GCTTCCATGG AACCTGCCGG 
TTTTTGGTGC AGGAAGAGAA GCCAGCATGT GTCTGCCACT CTGGGTACGT GGGTGTTCGC 
TGTGAGCATG CAGACCTCCT GGCTGTGGTG GCTGCCAGCC AGAAGAAGCA AGCCATCACT 
GCCCTGGTGG TGGTCTCCAT TGTGGCCCTG GCTGTCCTCA TTATCACCTG TGTGCTGATC 
CACTGCTGTC AGCTCCGCAA ACACTGTGAG TGGTGCCGTG CCCTCGTCTG CAGACATGAG 
AAGCCCAGCG CCCTCCTGAA GGGAAGGACT GCTTGCTGCC ACTCTGAGAC AGTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TGFA ( NM_031199.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_112476.1 Mus musculus transforming growth factor alpha (Tgfa), mRNA.

Price & Availability↑

CloneID	OMu15684
Clone ID Related Accession (Same CDS sequence)	NM_031199.4 , NM_031199.3
Accession Version	NM_031199.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	protransforming growth factor alpha preproprotein
Comment

ATGGTCCCCG CGACCGGACA GCTCGCTCTG CTAGCGCTGG GTATCCTGTT AGCTGTGTGC 
CAGGCTCTGG AGAACAGCAC ATCCCCCCTG AGTGACTCAC CCGTGGCGGC TGCAGTGGTG 
TCTCACTTCA ACAAGTGCCC AGATTCCCAC ACTCAGTACT GCTTCCATGG AACCTGCCGG 
TTTTTGGTGC AGGAAGAGAA GCCAGCATGT GTCTGCCACT CTGGGTACGT GGGTGTTCGC 
TGTGAGCATG CAGACCTCCT GGCTGTGGTG GCTGCCAGCC AGAAGAAGCA AGCCATCACT 
GCCCTGGTGG TGGTCTCCAT TGTGGCCCTG GCTGTCCTCA TTATCACCTG TGTGCTGATC 
CACTGCTGTC AGCTCCGCAA ACACTGTGAG TGGTGCCGTG CCCTCGTCTG CAGACATGAG 
AAGCCCAGCG CCCTCCTGAA GGGAAGGACT GCTTGCTGCC ACTCTGAGAC AGTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112476.1
Translation	MVPATGQLALLALGILLAVCQALENSTSPLSDSPVAAAVVSHFNKCPDSHTQYCFHGTCRFLVQEEKPACVCHSGYVGVRCEHADLLAVVAASQKKQAITALVVVSIVALAVLIITCVLIHCCQLRKHCEWCRALVCRHEKPSALLKGRTACCHSETVV

Target ORF information:

RefSeq Version	NM_031199.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus transforming growth factor alpha (Tgfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031199.3

ORF Insert Sequence:

ATGGTCCCCG CGACCGGACA GCTCGCTCTG CTAGCGCTGG GTATCCTGTT AGCTGTGTGC 
CAGGCTCTGG AGAACAGCAC ATCCCCCCTG AGTGACTCAC CCGTGGCGGC TGCAGTGGTG 
TCTCACTTCA ACAAGTGCCC AGATTCCCAC ACTCAGTACT GCTTCCATGG AACCTGCCGG 
TTTTTGGTGC AGGAAGAGAA GCCAGCATGT GTCTGCCACT CTGGGTACGT GGGTGTTCGC 
TGTGAGCATG CAGACCTCCT GGCTGTGGTG GCTGCCAGCC AGAAGAAGCA AGCCATCACT 
GCCCTGGTGG TGGTCTCCAT TGTGGCCCTG GCTGTCCTCA TTATCACCTG TGTGCTGATC 
CACTGCTGTC AGCTCCGCAA ACACTGTGAG TGGTGCCGTG CCCTCGTCTG CAGACATGAG 
AAGCCCAGCG CCCTCCTGAA GGGAAGGACT GCTTGCTGCC ACTCTGAGAC AGTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TGFA ( XM_030255312.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030111172.1 Mus musculus transforming growth factor alpha (Tgfa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu89177
Clone ID Related Accession (Same CDS sequence)	XM_030255312.1
Accession Version	XM_030255312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protransforming growth factor alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCCG CGACCGGACA GCTCGCTCTG CTAGCGCTGG GTATCCTGTT AGCTGTGTGC 
CAGGCTCTGG AGAACAGCAC ATCCCCCCTG AGTGACTCAC CCGTGGCGGC TGCAGTGGTG 
TCTCACTTCA ACAAGTGCCC AGATTCCCAC ACTCAGTACT GCTTCCATGG AACCTGCCGG 
TTTTTGGTGC AGGAAGAGAA GCCAGCATGT GTCTGCCACT CTGGGTACGT GGGTGTTCGC 
TGTGAGCATG CAGACCTCCT GGCTGTGGTG GCTGCCAGCC AGAAGAAGCA AGCCATCACT 
GCCCTGGTGG TGGTCTCCAT TGTGGCCCTG GCTGTCCTCA TTATCACCTG TGTGCTGATC 
CACTGCTGTC AGCTCCGCAA ACACTGTGAG TGGTGCCGTG CCCTCGTCTG CAGACATGAG 
AAGCCCAGCG CCCTCCTGAA GGGAAGGACT GCTTGCTGCC ACTCTGAGAC AGGTACAGGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111172.1
CDS	469..951
Translation	MVPATGQLALLALGILLAVCQALENSTSPLSDSPVAAAVVSHFNKCPDSHTQYCFHGTCRFLVQEEKPACVCHSGYVGVRCEHADLLAVVAASQKKQAITALVVVSIVALAVLIITCVLIHCCQLRKHCEWCRALVCRHEKPSALLKGRTACCHSETGTG

Target ORF information:

RefSeq Version	XM_030255312.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transforming growth factor alpha (Tgfa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255312.1

ORF Insert Sequence:

ATGGTCCCCG CGACCGGACA GCTCGCTCTG CTAGCGCTGG GTATCCTGTT AGCTGTGTGC 
CAGGCTCTGG AGAACAGCAC ATCCCCCCTG AGTGACTCAC CCGTGGCGGC TGCAGTGGTG 
TCTCACTTCA ACAAGTGCCC AGATTCCCAC ACTCAGTACT GCTTCCATGG AACCTGCCGG 
TTTTTGGTGC AGGAAGAGAA GCCAGCATGT GTCTGCCACT CTGGGTACGT GGGTGTTCGC 
TGTGAGCATG CAGACCTCCT GGCTGTGGTG GCTGCCAGCC AGAAGAAGCA AGCCATCACT 
GCCCTGGTGG TGGTCTCCAT TGTGGCCCTG GCTGTCCTCA TTATCACCTG TGTGCTGATC 
CACTGCTGTC AGCTCCGCAA ACACTGTGAG TGGTGCCGTG CCCTCGTCTG CAGACATGAG 
AAGCCCAGCG CCCTCCTGAA GGGAAGGACT GCTTGCTGCC ACTCTGAGAC AGGTACAGGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of TGF-alpha in the progression of diabetic kidney disease.
American journal of physiology. Renal physiology312(6)F951-F962(2017 Jun)
Heuer JG,Harlan SM,Yang DD,Jaqua DL,Boyles JS,Wilson JM,Heinz-Taheny KM,Sullivan JM,Wei T,Qian HR,Witcher DR,Breyer MD

Context-specific protection of TGF? null mice from osteoarthritis.
Scientific reports630434(2016 Jul)
Usmani SE,Ulici V,Pest MA,Hill TL,Welch ID,Beier F

Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGF?.
Scientific reports627342(2016 Jun)
Wunderle L,Knopf JD,K?hnle N,Morl? A,Hehn B,Adrain C,Strisovsky K,Freeman M,Lemberg MK

Mechanisms of Lung Fibrosis Resolution.
The American journal of pathology186(5)1066-77(2016 May)
Glasser SW,Hagood JS,Wong S,Taype CA,Madala SK,Hardie WD

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TGFA cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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