L2hgdh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	L2hgdh
Entrez Gene ID	217666
Full Name	L-2-hydroxyglutarate dehydrogenase
Synonyms	BC016226
General protein information	Preferred Names L-2-hydroxyglutarate dehydrogenase Names L-2-hydroxyglutarate dehydrogenase, mitochondrial duranin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_145443.2	NP_663418.1	L-2-hydroxyglutarate dehydrogenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	L2HGDH	NP_079160.1
Canis lupus familiaris (dog)	L2HGDH	XP_863530.2
Drosophila melanogaster (fruit fly)	CG10639	NP_609923.2
	L2HGDH	XP_001099959.1
Bos taurus (cattle)	L2HGDH	NP_001094560.1
Gallus gallus (chicken)	L2HGDH	XP_421462.2
Danio rerio (zebrafish)	l2hgdh	NP_001139067.1
Rattus norvegicus (Norway rat)	L2hgdh	NP_001101498.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007868	XP_317624.2
Caenorhabditis elegans (roundworm)	Y45G12B.3	NP_503730.1
Xenopus tropicalis (tropical clawed frog)	l2hgdh	XP_002938171.1
Pan troglodytes (chimpanzee)	L2HGDH	XP_001154304.2
Mus musculus (house mouse)	L2hgdh	NP_663418.1

Gene Ontology
Bibliography

Related articles in PubMed

Ma S, Sun R, Jiang B, Gao J, Deng W, Liu P, He R, Cui J, Ji M, Yi W, Yang P, Wu X, Xiong Y, Qiu Z, Ye D, Guan KL
Molecular and cellular biology37(8)(2017 Apr)

A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair.
Rzem R, Achouri Y, Marbaix E, Schakman O, Wiame E, Marie S, Gailly P, Vincent MF, Veiga-da-Cunha M, Van Schaftingen E
PloS one10(3)e0119540(2015)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduria.
Rzem R, Veiga-da-Cunha M, No?l G, Goffette S, Nassogne MC, Tabarki B, Sch?ller C, Marquardt T, Vikkula M, Van Schaftingen E
Proceedings of the National Academy of Sciences of the United States of America101(48)16849-54(2004 Nov)

Exchangeable gene trap using the Cre/mutated lox system.
Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki M, Miura K, Araki M, Yamamura K
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

L2hgdh mutation leads to l-2-hydroxyglutarate accumulation, leukoencephalopathy, and neurodegeneration in mice.

Data describe a mouse model of L-2-hydroxyglutaric aciduria where mouse with l2hgdh gene deficiency accumulates toxic levels of L-2-hydroxyglutarate mostly in brain and testis and shows significant brain spongiosis and learning deficit.
Title: A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair.

The following L2hgdh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the L2hgdh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13384	NM_145443.2 Latest version!	Mus musculus L-2-hydroxyglutarate dehydrogenase (L2hgdh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

L2hgdh ( NM_145443.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663418.1 Mus musculus L-2-hydroxyglutarate dehydrogenase (L2hgdh), mRNA.

Price & Availability↑

CloneID	OMu13384
Clone ID Related Accession (Same CDS sequence)	NM_145443.2
Accession Version	NM_145443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	L-2-hydroxyglutarate dehydrogenase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ087348.1, AK168429.1 and AK152450.1. On Oct 27, 2009 this sequence version replaced NM_145443.1. ##Evidence-Data-START## Transcript exon combination :: AK168429.1, AK145228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTGGCCGA CCCTGCGCTA CGTAGGCGGT GTCTGCGGAC TGGCCCGGTA CTGCGTGGCT 
GGGGGCTTCC TCCGCGCAAG TGGACCGGCG TCCGGGGTGC CCGGGCTACT GTGCGGGGGT 
GGCCGCAGAA GCTCCAGCAC CAGCTCTTTT GATATAGTCA TCGTTGGAGG TGGAATTGTG 
GGCCTTGCCT CTGCCAGAAC GCTCATCCTG AAACATCCTG GACTTTCGAT TGGTGTGGTG 
GAAAAGGAGA AAGATTTAGC TCTTCACCAG ACTGGACACA ACAGCGGTGT CATACACAGT 
GGTATTTACT ATAAACCAGA ATCTCTGAAG GCTAAATTGT GTGTAGAAGG CGCAGCCCTC 
ATCTATGAGT ACTGCAACCT CAAGGGAATT CCCTACAGGC AATGTGGCAA GCTAATAGTA 
GCTGTTGAAC AAGAAGAAAT TCCCAGACTT CAGGCCTTGT ATGAGAGAGG CCTGCAGAAT 
GGAGTCGAAG GCCTGAGGCT GATCCAGCAG GAGGATATAA AAAAGAAGGA GCCGTATTGC 
AGGGGTCTAA TGGCTATTGA TTGTCCATAC ACCGGCATTG TGAACTACCA ACAGGTAGCT 
TTGTCATTTG CCCAGGATTT CCAAGAAGCA GGTGGCTCTA TCTTGAGAGA TTTTGAGGTA 
AAAGGTATTG AAATAGCTAA AGAAAACTCT TCAAGAAGTA AAGATGGCAT GAACTATCCA 
ATCGCTGTCA AGAACAGCAA GGGAAAGGAG ATTCGGTGTC GATATGTTGT CACATGTGCT 
GGTCTTTACT CTGACCGTAT TTCAGAGTTG AGTGGCTGCA ATCCTGACCC TCAAATTGTC 
CCATTCCGGG GAGATTACCT GGTCTTAAAG CCAGAAAAGG GCTACCTTGT AAAAGGAAAT 
ATTTATCCGG TCCCAGACAG CCGGTTTCCT TTCCTTGGAG TTCACTTTAC GCCCAGGTTG 
GATGGCACTA TTTGGCTAGG GCCTAATGCA GTGCTTGCCT TTAAACGGGA AGGTTACAGA 
CCCTTTGACT TCGATGCCAG AGATGTTATG GAAGTAATTC TCAAAAGCGG CTTCATTAAC 
CTGGTGTTCC AGCATTTCTC TTATGGAGTT AATGAAATGT ATAAAGCCTG TTTTCTCAGT 
GAAACAGTGA AGCATCTTCA AAAGTTCATT CCTGAAATCA CCATCAGCGA TGTCCTTCGA 
GGTCCTGCTG GAGTACGGGC CCAGGCCCTG GACAGAGATG GAAATCTGGT AGAGGATTTT 
GTGTTTGACG GAGGGACTGG CGAGATTGCA GACCGCGTCC TTCATGTGAG AAATGCGCCT 
TCGCCTGCTG CCACTTCCTC CCTGGCAATT TCTAGAATGA TAGCAGAGGA AGCACAGCAA 
AGGTTTAAGT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663418.1
CDS	79..1473
Translation	MWPTLRYVGGVCGLARYCVAGGFLRASGPASGVPGLLCGGGRRSSSTSSFDIVIVGGGIVGLASARTLILKHPGLSIGVVEKEKDLALHQTGHNSGVIHSGIYYKPESLKAKLCVEGAALIYEYCNLKGIPYRQCGKLIVAVEQEEIPRLQALYERGLQNGVEGLRLIQQEDIKKKEPYCRGLMAIDCPYTGIVNYQQVALSFAQDFQEAGGSILRDFEVKGIEIAKENSSRSKDGMNYPIAVKNSKGKEIRCRYVVTCAGLYSDRISELSGCNPDPQIVPFRGDYLVLKPEKGYLVKGNIYPVPDSRFPFLGVHFTPRLDGTIWLGPNAVLAFKREGYRPFDFDARDVMEVILKSGFINLVFQHFSYGVNEMYKACFLSETVKHLQKFIPEITISDVLRGPAGVRAQALDRDGNLVEDFVFDGGTGEIADRVLHVRNAPSPAATSSLAISRMIAEEAQQRFKL

Target ORF information:

RefSeq Version	NM_145443.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus L-2-hydroxyglutarate dehydrogenase (L2hgdh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145443.2

ORF Insert Sequence:

ATGTGGCCGA CCCTGCGCTA CGTAGGCGGT GTCTGCGGAC TGGCCCGGTA CTGCGTGGCT 
GGGGGCTTCC TCCGCGCAAG TGGACCGGCG TCCGGGGTGC CCGGGCTACT GTGCGGGGGT 
GGCCGCAGAA GCTCCAGCAC CAGCTCTTTT GATATAGTCA TCGTTGGAGG TGGAATTGTG 
GGCCTTGCCT CTGCCAGAAC GCTCATCCTG AAACATCCTG GACTTTCGAT TGGTGTGGTG 
GAAAAGGAGA AAGATTTAGC TCTTCACCAG ACTGGACACA ACAGCGGTGT CATACACAGT 
GGTATTTACT ATAAACCAGA ATCTCTGAAG GCTAAATTGT GTGTAGAAGG CGCAGCCCTC 
ATCTATGAGT ACTGCAACCT CAAGGGAATT CCCTACAGGC AATGTGGCAA GCTAATAGTA 
GCTGTTGAAC AAGAAGAAAT TCCCAGACTT CAGGCCTTGT ATGAGAGAGG CCTGCAGAAT 
GGAGTCGAAG GCCTGAGGCT GATCCAGCAG GAGGATATAA AAAAGAAGGA GCCGTATTGC 
AGGGGTCTAA TGGCTATTGA TTGTCCATAC ACCGGCATTG TGAACTACCA ACAGGTAGCT 
TTGTCATTTG CCCAGGATTT CCAAGAAGCA GGTGGCTCTA TCTTGAGAGA TTTTGAGGTA 
AAAGGTATTG AAATAGCTAA AGAAAACTCT TCAAGAAGTA AAGATGGCAT GAACTATCCA 
ATCGCTGTCA AGAACAGCAA GGGAAAGGAG ATTCGGTGTC GATATGTTGT CACATGTGCT 
GGTCTTTACT CTGACCGTAT TTCAGAGTTG AGTGGCTGCA ATCCTGACCC TCAAATTGTC 
CCATTCCGGG GAGATTACCT GGTCTTAAAG CCAGAAAAGG GCTACCTTGT AAAAGGAAAT 
ATTTATCCGG TCCCAGACAG CCGGTTTCCT TTCCTTGGAG TTCACTTTAC GCCCAGGTTG 
GATGGCACTA TTTGGCTAGG GCCTAATGCA GTGCTTGCCT TTAAACGGGA AGGTTACAGA 
CCCTTTGACT TCGATGCCAG AGATGTTATG GAAGTAATTC TCAAAAGCGG CTTCATTAAC 
CTGGTGTTCC AGCATTTCTC TTATGGAGTT AATGAAATGT ATAAAGCCTG TTTTCTCAGT 
GAAACAGTGA AGCATCTTCA AAAGTTCATT CCTGAAATCA CCATCAGCGA TGTCCTTCGA 
GGTCCTGCTG GAGTACGGGC CCAGGCCCTG GACAGAGATG GAAATCTGGT AGAGGATTTT 
GTGTTTGACG GAGGGACTGG CGAGATTGCA GACCGCGTCC TTCATGTGAG AAATGCGCCT 
TCGCCTGCTG CCACTTCCTC CCTGGCAATT TCTAGAATGA TAGCAGAGGA AGCACAGCAA 
AGGTTTAAGT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular and cellular biology37(8)(2017 Apr)
Ma S,Sun R,Jiang B,Gao J,Deng W,Liu P,He R,Cui J,Ji M,Yi W,Yang P,Wu X,Xiong Y,Qiu Z,Ye D,Guan KL

A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair.
PloS one10(3)e0119540(2015)
Rzem R,Achouri Y,Marbaix E,Schakman O,Wiame E,Marie S,Gailly P,Vincent MF,Veiga-da-Cunha M,Van Schaftingen E

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduria.
Proceedings of the National Academy of Sciences of the United States of America101(48)16849-54(2004 Nov)
Rzem R,Veiga-da-Cunha M,No?l G,Goffette S,Nassogne MC,Tabarki B,Sch?ller C,Marquardt T,Vikkula M,Van Schaftingen E

Exchangeable gene trap using the Cre/mutated lox system.
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)
Araki K,Imaizumi T,Sekimoto T,Yoshinobu K,Yoshimuta J,Akizuki M,Miura K,Araki M,Yamamura K

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L2hgdh cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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