Nlrp3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlrp3
Entrez Gene ID	216799
Full Name	NLR family, pyrin domain containing 3
Synonyms	AGTAVPRL,AII/AVP,Cias1,FCAS,FCU,MWS,Mmig1,NALP3,Pypaf1
General protein information	Preferred Names NLR family, pyrin domain containing 3 Names NACHT, LRR and PYD domains-containing protein 3 NACHT/LRR/pyrin domain-containing protein 3 PYRIN-containing APAF1-like protein 1 cold autoinflammatory syndrome 1 protein homolog cryopyrin mast cell maturation-associated-inducible protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030245803.1	XP_030101663.1	NACHT, LRR and PYD domains-containing protein 3 isoform X2
NM_001359638.1	NP_001346567.1	NACHT, LRR and PYD domains-containing protein 3
XM_006532859.1	XP_006532922.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
XM_006532857.1	XP_006532920.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
XM_006532858.1	XP_006532921.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
NM_145827.3	NP_665826.1	NACHT, LRR and PYD domains-containing protein 3
NM_145827.4	NP_665826.1	NACHT, LRR and PYD domains-containing protein 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	NLRP3	NP_004886.3
	NLRP3	NP_001107823.1
Xenopus tropicalis (tropical clawed frog)	LOC100487412	XP_002938247.2
Xenopus tropicalis (tropical clawed frog)	LOC101730849	XP_004917660.1
Xenopus tropicalis (tropical clawed frog)	LOC100488807	XP_002943171.1
Rattus norvegicus (Norway rat)	Nlrp3	NP_001178571.1
Xenopus tropicalis (tropical clawed frog)	LOC101731251	XP_004911151.1
Xenopus tropicalis (tropical clawed frog)	LOC100489137	XP_002943173.1
Canis lupus familiaris (dog)	NLRP3	XP_848377.2
Mus musculus (house mouse)	Nlrp3	NP_665826.1
Xenopus tropicalis (tropical clawed frog)	LOC101730480	XP_004917655.1
Xenopus tropicalis (tropical clawed frog)	LOC100488969	XP_002943172.2
Pan troglodytes (chimpanzee)	NLRP3	XP_003949788.1
Xenopus tropicalis (tropical clawed frog)	LOC100491706	XP_002944439.2
Xenopus tropicalis (tropical clawed frog)	LOC100495083	XP_002940532.2
Xenopus tropicalis (tropical clawed frog)	LOC100486737	XP_002937318.2
Xenopus tropicalis (tropical clawed frog)	LOC101730955	XP_004917628.1
Xenopus tropicalis (tropical clawed frog)	LOC101730774	XP_004917659.1
Bos taurus (cattle)	NLRP3	NP_001095689.1
Gallus gallus (chicken)	NLRP3	XP_001233262.3
Xenopus tropicalis (tropical clawed frog)	LOC100497524	XP_002945565.2
Xenopus tropicalis (tropical clawed frog)	LOC100486578	XP_002937317.2
Xenopus tropicalis (tropical clawed frog)	LOC100493406	XP_004917630.1
Xenopus tropicalis (tropical clawed frog)	LOC100488643	XP_002943170.2

Gene Ontology

The following Nlrp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlrp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu85722	XM_030245803.1 Latest version!	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu18240	NM_145827.4 Latest version!	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu18240	NM_001359638.1 Latest version!	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu18240	XM_006532857.1 Latest version!	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu18240	XM_006532858.1 Latest version!	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu18240	XM_006532859.1 Latest version!	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
View Old Accession Versions >>
OMu18240	NM_145827.3	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlrp3 ( XM_030245803.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101663.1 Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X2, mRNA.

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CloneID	OMu85722
Clone ID Related Accession (Same CDS sequence)	XM_030245803.1
Accession Version	XM_030245803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATGCC AGGAGGACAG CCTTGAAGAA GAGTGGATGG GTTTGCTGGG ATATCTCTCC 
CGCATCTCCA TTTGTAAAAA GAAGAAAGAT TACTGTAAGA TGTACAGACG ACATGTGAGA 
AGCAGGTTCT ACTCTATCAA GGACAGGAAC GCGCGTCTAG GTGAGAGTGT GGACCTCAAC 
AGTCGCTACA CGCAGCTCCA ACTGGTCAAG GAGCATCCAA GCAAGCAGGA GCGGGAGCAT 
GAACTCCTGA CCATCGGCCG GACTAAAATG CGGGACAGCC CCATGAGTTC CCTTAAGCTG 
GAGCTGCTGT TTGAGCCCGA GGACGGGCAC TCGGAGCCTG TGCACACAGT GGTGTTCCAG 
GGAGCAGCAG GCATCGGGAA AACCATCCTA GCCAGGAAGA TTATGTTGGA CTGGGCACTG 
GGAAAGCTCT TCAAAGACAA ATTTGACTAT TTGTTCTTTA TCCACTGCCG AGAGGTGAGC 
CTCAGGACGC CAAGGAGTCT AGCAGACCTG ATTGTCAGCT GCTGGCCTGA CCCAAACCCA 
CCAGTGTGCA AGATCCTGCG CAAGCCTTCC AGGATCCTCT TCCTCATGGA TGGCTTTGAT 
GAGCTACAAG GGGCCTTTGA CGAGCACATT GGGGAGGTCT GCACAGACTG GCAAAAGGCT 
GTGCGGGGAG ACATTCTGCT AAGCAGCCTC ATCCGAAAGA AACTGCTGCC CAAGGCCTCT 
CTGCTCATAA CGACGAGGCC GGTAGCCTTG GAGAAACTGC AGCATCTCCT GGACCACCCC 
CGCCATGTGG AGATCCTAGG TTTCTCTGAG GCCAAAAGGA AGGAGTATTT CTTTAAGTAT 
TTCTCCAACG AGCTGCAGGC CCGGGAGGCC TTCAGGCTGA TCCAAGAGAA TGAGGTCCTC 
TTTACCATGT GCTTCATCCC CCTGGTCTGC TGGATTGTGT GCACGGGGCT AAAGCAACAG 
ATGGAGACCG GGAAGAGCCT GGCCCAGACC TCCAAGACCA CTACGGCCGT CTACGTCTTC 
TTCCTTTCCA GCCTGCTGCA ATCCCGGGGG GGCATTGAGG AGCATCTCTT CTCTGACTAC 
CTACAGGGGC TCTGTTCACT GGCTGCGGAT GGAATTTGGA ACCAGAAAAT CCTATTTGAG 
GAGTGTGATC TGCGGAAGCA CGGCCTGCAG AAGACTGACG TCTCCGCTTT CCTGAGGATG 
AACGTGTTCC AGAAGGAAGT GGACTGCGAG AGATTCTACA GCTTCAGCCA CATGACTTTC 
CAGGAGTTCT TCGCTGCTAT GTACTATTTG CTGGAAGAGG AGGCAGAGGG GGAGACCGTG 
AGGAAAGGAC CAGGAGGTTG TTCAGATCTT CTGAACCGAG ACGTGAAGGT CCTACTAGAA 
AATTACGGCA AGTTTGAAAA AGGCTATCTG ATTTTTGTTG TCCGATTCCT CTTTGGCCTT 
GTAAACCAGG AGAGAACCTC TTATTTGGAG AAGAAACTAA GTTGCAAGAT CTCTCAGCAA 
GTCAGACTGG AACTACTGAA GTGGATTGAA GTGAAAGCCA AGGCCAAGAA GCTGCAGTGG 
CAGCCCAGCC AACTGGAACT GTTCTACTGC CTGTACGAGA TGCAGGAGGA AGACTTTGTG 
CAGAGTGCCA TGGACCACTT TCCCAAAATT GAGATCAACC TCTCTACCAG AATGGACCAC 
GTGGTTTCCT CCTTTTGTAT TAAGAACTGT CATAGGGTCA AAACGCTTTC CCTGGGTTTT 
TTTCACAACT CGCCCAAGGA GGAAGAAGAA GAGAGGAGAG GAGGTCGACC CTTGGACCAG 
GTTCAGTGTG TTTTCCCAGA CACTCATGTT GCCTGTTCTT CCAGACTGGT GAACTGCTGC 
CTCACTTCTA GCTTCTGCCG TGGTCTCTTC TCAAGTCTAA GCACCAACCG GAGCCTCACT 
GAACTGGACC TCAGTGACAA TACTCTGGGA GACCCGGGCA TGAGGGTGCT GTGTGAGGCA 
CTCCAGCACC CAGGCTGTAA CATTCAGAGA CTGTGGTTGG GGCGCTGCGG ACTGTCCCAT 
CAATGCTGCT TCGACATCTC CTCTGTCCTG AGCAGCAGCC AGAAGCTGGT GGAGCTGGAC 
CTCAGTGACA ATGCCCTGGG GGACTTTGGA ATCAGATTGC TGTGTGTGGG ACTGAAGCAC 
CTGCTCTGCA ACCTCCAGAA ACTGTGGTTG GTGAGCTGCT GTCTCACATC CGCGTGTTGT 
CAGGATCTCG CATTGGTTCT GAGCTCCAAC CATTCTCTGA CCAGACTGTA CATTGGAGAA 
AATGCCTTGG GAGACTCAGG AGTCCAAGTT TTGTGTGAAA AGATGAAGGA CCCACAGTGT 
AACTTGCAGA AGCTGGGGTT GGTGAATTCC GGCCTTACTT CAATCTGTTG TTCAGCTCTG 
ACCTCTGTGC TCAAAACCAA CCAGAACTTC ACACACCTCT ATCTACGAAG CAATGCCCTT 
GGAGACACAG GACTCAGGCT CCTCTGTGAG GGGCTTCTGC ACCCGGACTG TAAACTACAG 
ATGCTGGAAT TAGACAACTG CAGCCTCACC TCACACAGCT GCTGGAATCT CTCCACAATT 
CTGACCCACA ACCACAGCCT TCGGAAGCTG AACCTGGGCA ACAATGATCT TGGCGATCTG 
TGCGTGGTGA CCCTCTGTGA GGTGCTGAAA CAGCAGGGCT GCCTCCTGCA GAGCCTACAG 
TTGGGTGAAA TGTACTTAAA TCGTGAAACA AAACGTGCCT TAGAAGCGCT CCAGGAAGAA 
AAGCCTGAGC TGACTATAGT CTTCGAGATT TCCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101663.1
CDS	272..3070
Translation	MVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKPSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGGIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLRKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGETVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLQKLWLVSCCLTSACCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRSNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW

Target ORF information:

RefSeq Version	XM_030245803.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245803.1

ORF Insert Sequence:

ATGGTATGCC AGGAGGACAG CCTTGAAGAA GAGTGGATGG GTTTGCTGGG ATATCTCTCC 
CGCATCTCCA TTTGTAAAAA GAAGAAAGAT TACTGTAAGA TGTACAGACG ACATGTGAGA 
AGCAGGTTCT ACTCTATCAA GGACAGGAAC GCGCGTCTAG GTGAGAGTGT GGACCTCAAC 
AGTCGCTACA CGCAGCTCCA ACTGGTCAAG GAGCATCCAA GCAAGCAGGA GCGGGAGCAT 
GAACTCCTGA CCATCGGCCG GACTAAAATG CGGGACAGCC CCATGAGTTC CCTTAAGCTG 
GAGCTGCTGT TTGAGCCCGA GGACGGGCAC TCGGAGCCTG TGCACACAGT GGTGTTCCAG 
GGAGCAGCAG GCATCGGGAA AACCATCCTA GCCAGGAAGA TTATGTTGGA CTGGGCACTG 
GGAAAGCTCT TCAAAGACAA ATTTGACTAT TTGTTCTTTA TCCACTGCCG AGAGGTGAGC 
CTCAGGACGC CAAGGAGTCT AGCAGACCTG ATTGTCAGCT GCTGGCCTGA CCCAAACCCA 
CCAGTGTGCA AGATCCTGCG CAAGCCTTCC AGGATCCTCT TCCTCATGGA TGGCTTTGAT 
GAGCTACAAG GGGCCTTTGA CGAGCACATT GGGGAGGTCT GCACAGACTG GCAAAAGGCT 
GTGCGGGGAG ACATTCTGCT AAGCAGCCTC ATCCGAAAGA AACTGCTGCC CAAGGCCTCT 
CTGCTCATAA CGACGAGGCC GGTAGCCTTG GAGAAACTGC AGCATCTCCT GGACCACCCC 
CGCCATGTGG AGATCCTAGG TTTCTCTGAG GCCAAAAGGA AGGAGTATTT CTTTAAGTAT 
TTCTCCAACG AGCTGCAGGC CCGGGAGGCC TTCAGGCTGA TCCAAGAGAA TGAGGTCCTC 
TTTACCATGT GCTTCATCCC CCTGGTCTGC TGGATTGTGT GCACGGGGCT AAAGCAACAG 
ATGGAGACCG GGAAGAGCCT GGCCCAGACC TCCAAGACCA CTACGGCCGT CTACGTCTTC 
TTCCTTTCCA GCCTGCTGCA ATCCCGGGGG GGCATTGAGG AGCATCTCTT CTCTGACTAC 
CTACAGGGGC TCTGTTCACT GGCTGCGGAT GGAATTTGGA ACCAGAAAAT CCTATTTGAG 
GAGTGTGATC TGCGGAAGCA CGGCCTGCAG AAGACTGACG TCTCCGCTTT CCTGAGGATG 
AACGTGTTCC AGAAGGAAGT GGACTGCGAG AGATTCTACA GCTTCAGCCA CATGACTTTC 
CAGGAGTTCT TCGCTGCTAT GTACTATTTG CTGGAAGAGG AGGCAGAGGG GGAGACCGTG 
AGGAAAGGAC CAGGAGGTTG TTCAGATCTT CTGAACCGAG ACGTGAAGGT CCTACTAGAA 
AATTACGGCA AGTTTGAAAA AGGCTATCTG ATTTTTGTTG TCCGATTCCT CTTTGGCCTT 
GTAAACCAGG AGAGAACCTC TTATTTGGAG AAGAAACTAA GTTGCAAGAT CTCTCAGCAA 
GTCAGACTGG AACTACTGAA GTGGATTGAA GTGAAAGCCA AGGCCAAGAA GCTGCAGTGG 
CAGCCCAGCC AACTGGAACT GTTCTACTGC CTGTACGAGA TGCAGGAGGA AGACTTTGTG 
CAGAGTGCCA TGGACCACTT TCCCAAAATT GAGATCAACC TCTCTACCAG AATGGACCAC 
GTGGTTTCCT CCTTTTGTAT TAAGAACTGT CATAGGGTCA AAACGCTTTC CCTGGGTTTT 
TTTCACAACT CGCCCAAGGA GGAAGAAGAA GAGAGGAGAG GAGGTCGACC CTTGGACCAG 
GTTCAGTGTG TTTTCCCAGA CACTCATGTT GCCTGTTCTT CCAGACTGGT GAACTGCTGC 
CTCACTTCTA GCTTCTGCCG TGGTCTCTTC TCAAGTCTAA GCACCAACCG GAGCCTCACT 
GAACTGGACC TCAGTGACAA TACTCTGGGA GACCCGGGCA TGAGGGTGCT GTGTGAGGCA 
CTCCAGCACC CAGGCTGTAA CATTCAGAGA CTGTGGTTGG GGCGCTGCGG ACTGTCCCAT 
CAATGCTGCT TCGACATCTC CTCTGTCCTG AGCAGCAGCC AGAAGCTGGT GGAGCTGGAC 
CTCAGTGACA ATGCCCTGGG GGACTTTGGA ATCAGATTGC TGTGTGTGGG ACTGAAGCAC 
CTGCTCTGCA ACCTCCAGAA ACTGTGGTTG GTGAGCTGCT GTCTCACATC CGCGTGTTGT 
CAGGATCTCG CATTGGTTCT GAGCTCCAAC CATTCTCTGA CCAGACTGTA CATTGGAGAA 
AATGCCTTGG GAGACTCAGG AGTCCAAGTT TTGTGTGAAA AGATGAAGGA CCCACAGTGT 
AACTTGCAGA AGCTGGGGTT GGTGAATTCC GGCCTTACTT CAATCTGTTG TTCAGCTCTG 
ACCTCTGTGC TCAAAACCAA CCAGAACTTC ACACACCTCT ATCTACGAAG CAATGCCCTT 
GGAGACACAG GACTCAGGCT CCTCTGTGAG GGGCTTCTGC ACCCGGACTG TAAACTACAG 
ATGCTGGAAT TAGACAACTG CAGCCTCACC TCACACAGCT GCTGGAATCT CTCCACAATT 
CTGACCCACA ACCACAGCCT TCGGAAGCTG AACCTGGGCA ACAATGATCT TGGCGATCTG 
TGCGTGGTGA CCCTCTGTGA GGTGCTGAAA CAGCAGGGCT GCCTCCTGCA GAGCCTACAG 
TTGGGTGAAA TGTACTTAAA TCGTGAAACA AAACGTGCCT TAGAAGCGCT CCAGGAAGAA 
AAGCCTGAGC TGACTATAGT CTTCGAGATT TCCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp3 ( NM_001359638.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346567.1 Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18240
Clone ID Related Accession (Same CDS sequence)	NM_001359638.1 , XM_006532859.1 , XM_006532857.1 , XM_006532858.1 , NM_145827.3 , NM_145827.4
Accession Version	NM_001359638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592522.12, BY186774.1, AY337285.1 and AW121591.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346567.1
CDS	139..3240
Translation	MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKPSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGGIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLRKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGETVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLQKLWLVSCCLTSACCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRSNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW

Target ORF information:

RefSeq Version	NM_001359638.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359638.1

ORF Insert Sequence:

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp3 ( NM_145827.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_665826.1 Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), mRNA.

Price & Availability↑

CloneID	OMu18240
Clone ID Related Accession (Same CDS sequence)	NM_001359638.1 , XM_006532859.1 , XM_006532857.1 , XM_006532858.1 , NM_145827.3 , NM_145827.4
Accession Version	NM_145827.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL592522.12. On Nov 17, 2006 this sequence version replaced NM_145827.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY495376.1, BC116174.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665826.1
CDS	227..3328
Translation	MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKPSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGGIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLRKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGETVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLQKLWLVSCCLTSACCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRSNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW

Target ORF information:

RefSeq Version	NM_145827.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145827.3

ORF Insert Sequence:

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp3 ( NM_145827.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_665826.1 Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18240
Clone ID Related Accession (Same CDS sequence)	NM_001359638.1 , XM_006532859.1 , XM_006532857.1 , XM_006532858.1 , NM_145827.3 , NM_145827.4
Accession Version	NM_145827.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592522.12, BY722759.1, AY337285.1 and AW121591.1. On Jan 13, 2018 this sequence version replaced NM_145827.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665826.1
CDS	227..3328
Translation	MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKPSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGGIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLRKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGETVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLQKLWLVSCCLTSACCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRSNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW

Target ORF information:

RefSeq Version	NM_145827.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145827.4

ORF Insert Sequence:

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp3 ( XM_006532859.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532922.1 Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu18240
Clone ID Related Accession (Same CDS sequence)	NM_001359638.1 , XM_006532859.1 , XM_006532857.1 , XM_006532858.1 , NM_145827.3 , NM_145827.4
Accession Version	XM_006532859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006532922.1

Translation

MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKPSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGGIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLRKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGETVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLQKLWLVSCCLTSACCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRSNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW

Target ORF information:

RefSeq Version	XM_006532859.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532859.1

ORF Insert Sequence:

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp3 ( XM_006532857.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532920.1 Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18240
Clone ID Related Accession (Same CDS sequence)	NM_001359638.1 , XM_006532859.1 , XM_006532857.1 , XM_006532858.1 , NM_145827.3 , NM_145827.4
Accession Version	XM_006532857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532920.1
CDS	139..3240
Translation	MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKPSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGGIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLRKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGETVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLQKLWLVSCCLTSACCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRSNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW

Target ORF information:

RefSeq Version	XM_006532857.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532857.1

ORF Insert Sequence:

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp3 ( XM_006532858.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532921.1 Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X1, mRNA.

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CloneID	OMu18240
Clone ID Related Accession (Same CDS sequence)	NM_001359638.1 , XM_006532859.1 , XM_006532857.1 , XM_006532858.1 , NM_145827.3 , NM_145827.4
Accession Version	XM_006532858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532921.1
CDS	136..3237
Translation	MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKPSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSNELQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMETGKSLAQTSKTTTAVYVFFLSSLLQSRGGIEEHLFSDYLQGLCSLAADGIWNQKILFEECDLRKHGLQKTDVSAFLRMNVFQKEVDCERFYSFSHMTFQEFFAAMYYLLEEEAEGETVRKGPGGCSDLLNRDVKVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQVRLELLKWIEVKAKAKKLQWQPSQLELFYCLYEMQEEDFVQSAMDHFPKIEINLSTRMDHVVSSFCIKNCHRVKTLSLGFFHNSPKEEEEERRGGRPLDQVQCVFPDTHVACSSRLVNCCLTSSFCRGLFSSLSTNRSLTELDLSDNTLGDPGMRVLCEALQHPGCNIQRLWLGRCGLSHQCCFDISSVLSSSQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLQKLWLVSCCLTSACCQDLALVLSSNHSLTRLYIGENALGDSGVQVLCEKMKDPQCNLQKLGLVNSGLTSICCSALTSVLKTNQNFTHLYLRSNALGDTGLRLLCEGLLHPDCKLQMLELDNCSLTSHSCWNLSTILTHNHSLRKLNLGNNDLGDLCVVTLCEVLKQQGCLLQSLQLGEMYLNRETKRALEALQEEKPELTIVFEISW

Target ORF information:

RefSeq Version	XM_006532858.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 3 (Nlrp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532858.1

ORF Insert Sequence:

ATGACGAGTG TCCGTTGCAA GCTGGCTCAG TATCTAGAGG ACCTTGAAGA TGTGGACCTC 
AAGAAATTCA AAATGCATTT GGAAGATTAC CCGCCCGAGA AAGGCTGTAT CCCAGTCCCC 
AGGGGCCAGA TGGAGAAGGC AGATCACTTG GATCTAGCCA CACTCATGAT TGACTTCAAT 
GGCGAGGAGA AGGCCTGGGC CATGGCTGTG TGGATCTTTG CTGCGATCAA CAGGCGAGAC 
CTCTGGGAAA AAGCTAAGAA GGACCAGCCA GAGTGGAATG ACACGTGTAC ATCACATTCC 
TCTATGGTAT GCCAGGAGGA CAGCCTTGAA GAAGAGTGGA TGGGTTTGCT GGGATATCTC 
TCCCGCATCT CCATTTGTAA AAAGAAGAAA GATTACTGTA AGATGTACAG ACGACATGTG 
AGAAGCAGGT TCTACTCTAT CAAGGACAGG AACGCGCGTC TAGGTGAGAG TGTGGACCTC 
AACAGTCGCT ACACGCAGCT CCAACTGGTC AAGGAGCATC CAAGCAAGCA GGAGCGGGAG 
CATGAACTCC TGACCATCGG CCGGACTAAA ATGCGGGACA GCCCCATGAG TTCCCTTAAG 
CTGGAGCTGC TGTTTGAGCC CGAGGACGGG CACTCGGAGC CTGTGCACAC AGTGGTGTTC 
CAGGGAGCAG CAGGCATCGG GAAAACCATC CTAGCCAGGA AGATTATGTT GGACTGGGCA 
CTGGGAAAGC TCTTCAAAGA CAAATTTGAC TATTTGTTCT TTATCCACTG CCGAGAGGTG 
AGCCTCAGGA CGCCAAGGAG TCTAGCAGAC CTGATTGTCA GCTGCTGGCC TGACCCAAAC 
CCACCAGTGT GCAAGATCCT GCGCAAGCCT TCCAGGATCC TCTTCCTCAT GGATGGCTTT 
GATGAGCTAC AAGGGGCCTT TGACGAGCAC ATTGGGGAGG TCTGCACAGA CTGGCAAAAG 
GCTGTGCGGG GAGACATTCT GCTAAGCAGC CTCATCCGAA AGAAACTGCT GCCCAAGGCC 
TCTCTGCTCA TAACGACGAG GCCGGTAGCC TTGGAGAAAC TGCAGCATCT CCTGGACCAC 
CCCCGCCATG TGGAGATCCT AGGTTTCTCT GAGGCCAAAA GGAAGGAGTA TTTCTTTAAG 
TATTTCTCCA ACGAGCTGCA GGCCCGGGAG GCCTTCAGGC TGATCCAAGA GAATGAGGTC 
CTCTTTACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATTG TGTGCACGGG GCTAAAGCAA 
CAGATGGAGA CCGGGAAGAG CCTGGCCCAG ACCTCCAAGA CCACTACGGC CGTCTACGTC 
TTCTTCCTTT CCAGCCTGCT GCAATCCCGG GGGGGCATTG AGGAGCATCT CTTCTCTGAC 
TACCTACAGG GGCTCTGTTC ACTGGCTGCG GATGGAATTT GGAACCAGAA AATCCTATTT 
GAGGAGTGTG ATCTGCGGAA GCACGGCCTG CAGAAGACTG ACGTCTCCGC TTTCCTGAGG 
ATGAACGTGT TCCAGAAGGA AGTGGACTGC GAGAGATTCT ACAGCTTCAG CCACATGACT 
TTCCAGGAGT TCTTCGCTGC TATGTACTAT TTGCTGGAAG AGGAGGCAGA GGGGGAGACC 
GTGAGGAAAG GACCAGGAGG TTGTTCAGAT CTTCTGAACC GAGACGTGAA GGTCCTACTA 
GAAAATTACG GCAAGTTTGA AAAAGGCTAT CTGATTTTTG TTGTCCGATT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC CTCTTATTTG GAGAAGAAAC TAAGTTGCAA GATCTCTCAG 
CAAGTCAGAC TGGAACTACT GAAGTGGATT GAAGTGAAAG CCAAGGCCAA GAAGCTGCAG 
TGGCAGCCCA GCCAACTGGA ACTGTTCTAC TGCCTGTACG AGATGCAGGA GGAAGACTTT 
GTGCAGAGTG CCATGGACCA CTTTCCCAAA ATTGAGATCA ACCTCTCTAC CAGAATGGAC 
CACGTGGTTT CCTCCTTTTG TATTAAGAAC TGTCATAGGG TCAAAACGCT TTCCCTGGGT 
TTTTTTCACA ACTCGCCCAA GGAGGAAGAA GAAGAGAGGA GAGGAGGTCG ACCCTTGGAC 
CAGGTTCAGT GTGTTTTCCC AGACACTCAT GTTGCCTGTT CTTCCAGACT GGTGAACTGC 
TGCCTCACTT CTAGCTTCTG CCGTGGTCTC TTCTCAAGTC TAAGCACCAA CCGGAGCCTC 
ACTGAACTGG ACCTCAGTGA CAATACTCTG GGAGACCCGG GCATGAGGGT GCTGTGTGAG 
GCACTCCAGC ACCCAGGCTG TAACATTCAG AGACTGTGGT TGGGGCGCTG CGGACTGTCC 
CATCAATGCT GCTTCGACAT CTCCTCTGTC CTGAGCAGCA GCCAGAAGCT GGTGGAGCTG 
GACCTCAGTG ACAATGCCCT GGGGGACTTT GGAATCAGAT TGCTGTGTGT GGGACTGAAG 
CACCTGCTCT GCAACCTCCA GAAACTGTGG TTGGTGAGCT GCTGTCTCAC ATCCGCGTGT 
TGTCAGGATC TCGCATTGGT TCTGAGCTCC AACCATTCTC TGACCAGACT GTACATTGGA 
GAAAATGCCT TGGGAGACTC AGGAGTCCAA GTTTTGTGTG AAAAGATGAA GGACCCACAG 
TGTAACTTGC AGAAGCTGGG GTTGGTGAAT TCCGGCCTTA CTTCAATCTG TTGTTCAGCT 
CTGACCTCTG TGCTCAAAAC CAACCAGAAC TTCACACACC TCTATCTACG AAGCAATGCC 
CTTGGAGACA CAGGACTCAG GCTCCTCTGT GAGGGGCTTC TGCACCCGGA CTGTAAACTA 
CAGATGCTGG AATTAGACAA CTGCAGCCTC ACCTCACACA GCTGCTGGAA TCTCTCCACA 
ATTCTGACCC ACAACCACAG CCTTCGGAAG CTGAACCTGG GCAACAATGA TCTTGGCGAT 
CTGTGCGTGG TGACCCTCTG TGAGGTGCTG AAACAGCAGG GCTGCCTCCT GCAGAGCCTA 
CAGTTGGGTG AAATGTACTT AAATCGTGAA ACAAAACGTG CCTTAGAAGC GCTCCAGGAA 
GAAAAGCCTG AGCTGACTAT AGTCTTCGAG ATTTCCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Nlrp3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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