Mrpl22 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrpl22
Entrez Gene ID	216767
Full Name	mitochondrial ribosomal protein L22
Synonyms	E030011D16Rik,HSPC158,MRP-L25,Rpml25
General protein information	Preferred Names mitochondrial ribosomal protein L22 Names 39S ribosomal protein L22, mitochondrial L22mt MRP-L22
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_175001.3	NP_778166.2	39S ribosomal protein L22, mitochondrial precursor
NM_175001.4	NP_778166.2	39S ribosomal protein L22, mitochondrial precursor
XM_006532854.4	XP_006532917.1	39S ribosomal protein L22, mitochondrial isoform X2
XM_006532854.3	XP_006532917.1	39S ribosomal protein L22, mitochondrial isoform X2
XM_017314447.1	XP_017169936.1	39S ribosomal protein L22, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MRPL22	NP_001030531.1
Mus musculus (house mouse)	Mrpl22	NP_778166.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000508	XP_001237272.3
	MRPL22	XP_001113082.1
Gallus gallus (chicken)	MRPL22	XP_414573.1
Danio rerio (zebrafish)	mrpl22	NP_001116093.1
Pan troglodytes (chimpanzee)	MRPL22	XP_003950713.1
Rattus norvegicus (Norway rat)	Mrpl22	NP_001099251.1
Canis lupus familiaris (dog)	MRPL22	XP_536458.3
Drosophila melanogaster (fruit fly)	mRpL22	NP_523379.2
Caenorhabditis elegans (roundworm)	mrpl-22	NP_499340.1
Xenopus tropicalis (tropical clawed frog)	mrpl22	NP_001072914.1
Homo sapiens (human)	MRPL22	NP_054899.2

Gene Ontology
Bibliography

Related articles in PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia.
Carninci P, Waki K, Shiraki T, Konno H, Shibata K, Itoh M, Aizawa K, Arakawa T, Ishii Y, Sasaki D, Bono H, Kondo S, Sugahara Y, Saito R, Osato N, Fukuda S, Sato K, Watahiki A, Hirozane-Kishikawa T, Nakamura M, Shibata Y, Yasunishi A, Kikuchi N, Yoshiki A, Kusakabe M, Gustincich S, Beisel K, Pavan W, Aidinis V, Nakagawara A, Held WA, Iwata H, Kono T, Nakauchi H, Lyons P, Wells C, Hume DA, Fagiolini M, Hensch TK, Brinkmeier M, Camper S, Hirota J, Mombaerts P, Muramatsu M, Okazaki Y, Kawai J, Hayashizaki Y
Genome research13(6B)1273-89(2003 Jun)

Mammalian mitochondrial ribosomal proteins. N-terminal amino acid sequencing, characterization, and identification of corresponding gene sequences.
Goldschmidt-Reisin S, Kitakawa M, Herfurth E, Wittmann-Liebold B, Grohmann L, Graack HR
The Journal of biological chemistry273(52)34828-36(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mrpl22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrpl22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18905	NM_175001.4 Latest version!	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu45995	XM_006532854.4 Latest version!	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu77843	XM_017314447.1 Latest version!	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu18905	NM_175001.3	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu45995	XM_006532854.3	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrpl22 ( XM_006532854.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532917.1 Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45995
Clone ID Related Accession (Same CDS sequence)	XM_006532854.4 , XM_006532854.3
Accession Version	XM_006532854.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	39S ribosomal protein L22, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTACC TGGCAAAGAT GATACGAGGC ATGTCCATTG ATCAGGCTTT GGCGCAGCTG 
GAGTTCAATG ATAAAAAGGG GGCTCAAATC ATTAAAGAGG TTCTCTTAGA AGCACAAGAC 
ATGGCAGTGA GAGACCATAA TGTGGAATTC AGATCCAACT TACACATAGC TGAGTCTACC 
TCAGGCCGAG GCCAGTGCCT GAAGCGCATC CGTTACCATG GCCGAGGTCG CTTTGGCATT 
ATGGAGAAGG TCTATTGCCA CTATTTTGTA AAGCTGGTGG AAGGACCCCC TCCCCCACCC 
GAAGTACCCA AGACAGCAGT TGACCACGCC AAGGACTATA TTCAGCAGCT TCGCAGCCGG 
ACCATCATCC ATACTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532917.1
CDS	502..882
Translation	MWYLAKMIRGMSIDQALAQLEFNDKKGAQIIKEVLLEAQDMAVRDHNVEFRSNLHIAESTSGRGQCLKRIRYHGRGRFGIMEKVYCHYFVKLVEGPPPPPEVPKTAVDHAKDYIQQLRSRTIIHTL

Target ORF information:

RefSeq Version	XM_006532854.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532854.3

ORF Insert Sequence:

ATGTGGTACC TGGCAAAGAT GATACGAGGC ATGTCCATTG ATCAGGCTTT GGCGCAGCTG 
GAGTTCAATG ATAAAAAGGG GGCTCAAATC ATTAAAGAGG TTCTCTTAGA AGCACAAGAC 
ATGGCAGTGA GAGACCATAA TGTGGAATTC AGATCCAACT TACACATAGC TGAGTCTACC 
TCAGGCCGAG GCCAGTGCCT GAAGCGCATC CGTTACCATG GCCGAGGTCG CTTTGGCATT 
ATGGAGAAGG TCTATTGCCA CTATTTTGTA AAGCTGGTGG AAGGACCCCC TCCCCCACCC 
GAAGTACCCA AGACAGCAGT TGACCACGCC AAGGACTATA TTCAGCAGCT TCGCAGCCGG 
ACCATCATCC ATACTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrpl22 ( NM_175001.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_778166.2 Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), mRNA.

Price & Availability↑

CloneID	OMu18905
Clone ID Related Accession (Same CDS sequence)	NM_175001.3 , NM_175001.4
Accession Version	NM_175001.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Mus musculus(house mouse)
Product	39S ribosomal protein L22, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY790081.1, AK086913.1 and AW986653.1. On Oct 31, 2009 this sequence version replaced NM_175001.2. ##Evidence-Data-START## Transcript exon combination :: AK077615.1, BI555394.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CACTACTGCG AGAGTTGGGT GCACTGAGGG TACCTAACCT GAGGATCTGG 
GCAACGCAGA CGCTGCGGGT TTTACCCCCG TCGTGCATCC ACACGAGTGC TTCTCTTGAC 
ATTTCTCGCA AATGGGAGAA GAAGAATAAG ATTGTTTACC CACCTCAACT GCCTGGCGAA 
CCTCGGAGAC CAGCGGAAAT TTACCATTGT CGGAGACAAA TAAAATACAG CAAGGACAAG 
ATGTGGTACC TGGCAAAGAT GATACGAGGC ATGTCCATTG ATCAGGCTTT GGCGCAGCTG 
GAGTTCAATG ATAAAAAGGG GGCTCAAATC ATTAAAGAGG TTCTCTTAGA AGCACAAGAC 
ATGGCAGTGA GAGACCATAA TGTGGAATTC AGATCCAACT TACACATAGC TGAGTCTACC 
TCAGGCCGAG GCCAGTGCCT GAAGCGCATC CGTTACCATG GCCGAGGTCG CTTTGGCATT 
ATGGAGAAGG TCTATTGCCA CTATTTTGTA AAGCTGGTGG AAGGACCCCC TCCCCCACCC 
GAAGTACCCA AGACAGCAGT TGACCACGCC AAGGACTATA TTCAGCAGCT TCGCAGCCGG 
ACCATCATCC ATACTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_778166.2
CDS	33..653
Translation	MAAALLRELGALRVPNLRIWATQTLRVLPPSCIHTSASLDISRKWEKKNKIVYPPQLPGEPRRPAEIYHCRRQIKYSKDKMWYLAKMIRGMSIDQALAQLEFNDKKGAQIIKEVLLEAQDMAVRDHNVEFRSNLHIAESTSGRGQCLKRIRYHGRGRFGIMEKVYCHYFVKLVEGPPPPPEVPKTAVDHAKDYIQQLRSRTIIHTL

Target ORF information:

RefSeq Version	NM_175001.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175001.3

ORF Insert Sequence:

ATGGCGGCCG CACTACTGCG AGAGTTGGGT GCACTGAGGG TACCTAACCT GAGGATCTGG 
GCAACGCAGA CGCTGCGGGT TTTACCCCCG TCGTGCATCC ACACGAGTGC TTCTCTTGAC 
ATTTCTCGCA AATGGGAGAA GAAGAATAAG ATTGTTTACC CACCTCAACT GCCTGGCGAA 
CCTCGGAGAC CAGCGGAAAT TTACCATTGT CGGAGACAAA TAAAATACAG CAAGGACAAG 
ATGTGGTACC TGGCAAAGAT GATACGAGGC ATGTCCATTG ATCAGGCTTT GGCGCAGCTG 
GAGTTCAATG ATAAAAAGGG GGCTCAAATC ATTAAAGAGG TTCTCTTAGA AGCACAAGAC 
ATGGCAGTGA GAGACCATAA TGTGGAATTC AGATCCAACT TACACATAGC TGAGTCTACC 
TCAGGCCGAG GCCAGTGCCT GAAGCGCATC CGTTACCATG GCCGAGGTCG CTTTGGCATT 
ATGGAGAAGG TCTATTGCCA CTATTTTGTA AAGCTGGTGG AAGGACCCCC TCCCCCACCC 
GAAGTACCCA AGACAGCAGT TGACCACGCC AAGGACTATA TTCAGCAGCT TCGCAGCCGG 
ACCATCATCC ATACTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrpl22 ( NM_175001.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_778166.2 Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), mRNA.

Price & Availability↑

CloneID	OMu18905
Clone ID Related Accession (Same CDS sequence)	NM_175001.3 , NM_175001.4
Accession Version	NM_175001.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	39S ribosomal protein L22, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY790081.1, AK086913.1 and AW986653.1. On Jun 8, 2018 this sequence version replaced NM_175001.3. ##Evidence-Data-START## Transcript exon combination :: AK077615.1, BI555394.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CACTACTGCG AGAGTTGGGT GCACTGAGGG TACCTAACCT GAGGATCTGG 
GCAACGCAGA CGCTGCGGGT TTTACCCCCG TCGTGCATCC ACACGAGTGC TTCTCTTGAC 
ATTTCTCGCA AATGGGAGAA GAAGAATAAG ATTGTTTACC CACCTCAACT GCCTGGCGAA 
CCTCGGAGAC CAGCGGAAAT TTACCATTGT CGGAGACAAA TAAAATACAG CAAGGACAAG 
ATGTGGTACC TGGCAAAGAT GATACGAGGC ATGTCCATTG ATCAGGCTTT GGCGCAGCTG 
GAGTTCAATG ATAAAAAGGG GGCTCAAATC ATTAAAGAGG TTCTCTTAGA AGCACAAGAC 
ATGGCAGTGA GAGACCATAA TGTGGAATTC AGATCCAACT TACACATAGC TGAGTCTACC 
TCAGGCCGAG GCCAGTGCCT GAAGCGCATC CGTTACCATG GCCGAGGTCG CTTTGGCATT 
ATGGAGAAGG TCTATTGCCA CTATTTTGTA AAGCTGGTGG AAGGACCCCC TCCCCCACCC 
GAAGTACCCA AGACAGCAGT TGACCACGCC AAGGACTATA TTCAGCAGCT TCGCAGCCGG 
ACCATCATCC ATACTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_778166.2
CDS	33..653
Translation	MAAALLRELGALRVPNLRIWATQTLRVLPPSCIHTSASLDISRKWEKKNKIVYPPQLPGEPRRPAEIYHCRRQIKYSKDKMWYLAKMIRGMSIDQALAQLEFNDKKGAQIIKEVLLEAQDMAVRDHNVEFRSNLHIAESTSGRGQCLKRIRYHGRGRFGIMEKVYCHYFVKLVEGPPPPPEVPKTAVDHAKDYIQQLRSRTIIHTL

Target ORF information:

RefSeq Version	NM_175001.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175001.4

ORF Insert Sequence:

ATGGCGGCCG CACTACTGCG AGAGTTGGGT GCACTGAGGG TACCTAACCT GAGGATCTGG 
GCAACGCAGA CGCTGCGGGT TTTACCCCCG TCGTGCATCC ACACGAGTGC TTCTCTTGAC 
ATTTCTCGCA AATGGGAGAA GAAGAATAAG ATTGTTTACC CACCTCAACT GCCTGGCGAA 
CCTCGGAGAC CAGCGGAAAT TTACCATTGT CGGAGACAAA TAAAATACAG CAAGGACAAG 
ATGTGGTACC TGGCAAAGAT GATACGAGGC ATGTCCATTG ATCAGGCTTT GGCGCAGCTG 
GAGTTCAATG ATAAAAAGGG GGCTCAAATC ATTAAAGAGG TTCTCTTAGA AGCACAAGAC 
ATGGCAGTGA GAGACCATAA TGTGGAATTC AGATCCAACT TACACATAGC TGAGTCTACC 
TCAGGCCGAG GCCAGTGCCT GAAGCGCATC CGTTACCATG GCCGAGGTCG CTTTGGCATT 
ATGGAGAAGG TCTATTGCCA CTATTTTGTA AAGCTGGTGG AAGGACCCCC TCCCCCACCC 
GAAGTACCCA AGACAGCAGT TGACCACGCC AAGGACTATA TTCAGCAGCT TCGCAGCCGG 
ACCATCATCC ATACTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrpl22 ( XM_006532854.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532917.1 Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45995
Clone ID Related Accession (Same CDS sequence)	XM_006532854.4 , XM_006532854.3
Accession Version	XM_006532854.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	39S ribosomal protein L22, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532854.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTACC TGGCAAAGAT GATACGAGGC ATGTCCATTG ATCAGGCTTT GGCGCAGCTG 
GAGTTCAATG ATAAAAAGGG GGCTCAAATC ATTAAAGAGG TTCTCTTAGA AGCACAAGAC 
ATGGCAGTGA GAGACCATAA TGTGGAATTC AGATCCAACT TACACATAGC TGAGTCTACC 
TCAGGCCGAG GCCAGTGCCT GAAGCGCATC CGTTACCATG GCCGAGGTCG CTTTGGCATT 
ATGGAGAAGG TCTATTGCCA CTATTTTGTA AAGCTGGTGG AAGGACCCCC TCCCCCACCC 
GAAGTACCCA AGACAGCAGT TGACCACGCC AAGGACTATA TTCAGCAGCT TCGCAGCCGG 
ACCATCATCC ATACTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532917.1
CDS	1079..1459
Translation	MWYLAKMIRGMSIDQALAQLEFNDKKGAQIIKEVLLEAQDMAVRDHNVEFRSNLHIAESTSGRGQCLKRIRYHGRGRFGIMEKVYCHYFVKLVEGPPPPPEVPKTAVDHAKDYIQQLRSRTIIHTL

Target ORF information:

RefSeq Version	XM_006532854.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532854.4

ORF Insert Sequence:

ATGTGGTACC TGGCAAAGAT GATACGAGGC ATGTCCATTG ATCAGGCTTT GGCGCAGCTG 
GAGTTCAATG ATAAAAAGGG GGCTCAAATC ATTAAAGAGG TTCTCTTAGA AGCACAAGAC 
ATGGCAGTGA GAGACCATAA TGTGGAATTC AGATCCAACT TACACATAGC TGAGTCTACC 
TCAGGCCGAG GCCAGTGCCT GAAGCGCATC CGTTACCATG GCCGAGGTCG CTTTGGCATT 
ATGGAGAAGG TCTATTGCCA CTATTTTGTA AAGCTGGTGG AAGGACCCCC TCCCCCACCC 
GAAGTACCCA AGACAGCAGT TGACCACGCC AAGGACTATA TTCAGCAGCT TCGCAGCCGG 
ACCATCATCC ATACTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrpl22 ( XM_017314447.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169936.1 Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77843
Clone ID Related Accession (Same CDS sequence)	XM_017314447.1
Accession Version	XM_017314447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	39S ribosomal protein L22, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTATAG TCAGCCGGCG TTTGATCCCG GAGGCTTCCG TAGGGCTCAG CATGGCGGCC 
GCACTACTGC GAGAGTTGGG TGCACTGAGG GTACCTAACC TGAGGATCTG GGCAACGCAG 
ACGCTGCGGG TTTTACCCCC GTCGTGCATC CACACGAGTG CTTCTCTTGA CATTTCTCGC 
AAATGGGAGA AGAAGAATAA GATTGTTTAC CCACCTCAAC TGCCTGGCGA ACCTCGGAGA 
CCAGCGGAAA TTTACCATTG TCGGAGACAA ATAAAATACA GCAAGGACAA GATGTGGTAC 
CTGGCAAAGA TGATACGAGG CATGTCCATT GATCAGGCTT TGGCGCAGCT GGAGTTCAAT 
GATAAAAAGG GGGCTCAAAT CATTAAAGAG GTTCTCTTAG AAGCACAAGA CATGGCAGTG 
AGAGACCATA ATGTGGAATT CAGATCCAAC TTACACATAG CTGAGTCTAC CTCAGGCCGA 
GGCCAGTGCC TGAAGCGCAT CCGTTACCAT GGCCGAGGTC GCTTTGGCAT TATGGAGAAG 
GTCTATTGCC ACTATTTTGT AAAGCTGGTG GAAGGACCCC CTCCCCCACC CGAAGTACCC 
AAGACAGCAG TTGACCACGC CAAGGACTAT ATTCAGCAGC TTCGCAGCCG GACCATCATC 
CATACTCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169936.1
CDS	614..1285
Translation	MRIVSRRLIPEASVGLSMAAALLRELGALRVPNLRIWATQTLRVLPPSCIHTSASLDISRKWEKKNKIVYPPQLPGEPRRPAEIYHCRRQIKYSKDKMWYLAKMIRGMSIDQALAQLEFNDKKGAQIIKEVLLEAQDMAVRDHNVEFRSNLHIAESTSGRGQCLKRIRYHGRGRFGIMEKVYCHYFVKLVEGPPPPPEVPKTAVDHAKDYIQQLRSRTIIHTL

Target ORF information:

RefSeq Version	XM_017314447.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein L22 (Mrpl22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314447.1

ORF Insert Sequence:

ATGCGTATAG TCAGCCGGCG TTTGATCCCG GAGGCTTCCG TAGGGCTCAG CATGGCGGCC 
GCACTACTGC GAGAGTTGGG TGCACTGAGG GTACCTAACC TGAGGATCTG GGCAACGCAG 
ACGCTGCGGG TTTTACCCCC GTCGTGCATC CACACGAGTG CTTCTCTTGA CATTTCTCGC 
AAATGGGAGA AGAAGAATAA GATTGTTTAC CCACCTCAAC TGCCTGGCGA ACCTCGGAGA 
CCAGCGGAAA TTTACCATTG TCGGAGACAA ATAAAATACA GCAAGGACAA GATGTGGTAC 
CTGGCAAAGA TGATACGAGG CATGTCCATT GATCAGGCTT TGGCGCAGCT GGAGTTCAAT 
GATAAAAAGG GGGCTCAAAT CATTAAAGAG GTTCTCTTAG AAGCACAAGA CATGGCAGTG 
AGAGACCATA ATGTGGAATT CAGATCCAAC TTACACATAG CTGAGTCTAC CTCAGGCCGA 
GGCCAGTGCC TGAAGCGCAT CCGTTACCAT GGCCGAGGTC GCTTTGGCAT TATGGAGAAG 
GTCTATTGCC ACTATTTTGT AAAGCTGGTG GAAGGACCCC CTCCCCCACC CGAAGTACCC 
AAGACAGCAG TTGACCACGC CAAGGACTAT ATTCAGCAGC TTCGCAGCCG GACCATCATC 
CATACTCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia.
Genome research13(6B)1273-89(2003 Jun)
Carninci P,Waki K,Shiraki T,Konno H,Shibata K,Itoh M,Aizawa K,Arakawa T,Ishii Y,Sasaki D,Bono H,Kondo S,Sugahara Y,Saito R,Osato N,Fukuda S,Sato K,Watahiki A,Hirozane-Kishikawa T,Nakamura M,Shibata Y,Yasunishi A,Kikuchi N,Yoshiki A,Kusakabe M,Gustincich S,Beisel K,Pavan W,Aidinis V,Nakagawara A,Held WA,Iwata H,Kono T,Nakauchi H,Lyons P,Wells C,Hume DA,Fagiolini M,Hensch TK,Brinkmeier M,Camper S,Hirota J,Mombaerts P,Muramatsu M,Okazaki Y,Kawai J,Hayashizaki Y

Mammalian mitochondrial ribosomal proteins. N-terminal amino acid sequencing, characterization, and identification of corresponding gene sequences.
The Journal of biological chemistry273(52)34828-36(1998 Dec)
Goldschmidt-Reisin S,Kitakawa M,Herfurth E,Wittmann-Liebold B,Grohmann L,Graack HR

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Mrpl22 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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