Aftph cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aftph
Entrez Gene ID	216549
Full Name	aftiphilin
General protein information	Preferred Names aftiphilin Names aftiphilin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 A3.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006514625.4	XP_006514688.1	aftiphilin isoform X1
NM_181411.4	NP_852076.1	aftiphilin isoform 1
NM_001252503.2	NP_001239432.1	aftiphilin isoform 2
NM_001290545.1	NP_001277474.1	aftiphilin isoform 3
XM_006514625.3	XP_006514688.1	aftiphilin isoform X1

Pathways from BioSystems

Homologies

	AFTPH	XP_001088472.1
Canis lupus familiaris (dog)	AFTPH	XP_005626216.1
Mus musculus (house mouse)	Aftph	NP_852076.1
Rattus norvegicus (Norway rat)	Aftph	XP_006251595.1
Danio rerio (zebrafish)	aftpha	NP_001139033.1
Homo sapiens (human)	AFTPH	NP_982261.2
Pan troglodytes (chimpanzee)	AFTPH	XP_001165739.1
Gallus gallus (chicken)	AFTPH	XP_419346.2
Bos taurus (cattle)	AFTPH	XP_005212889.1

Gene Ontology
Bibliography

Related articles in PubMed

Aftiphilin is a component of the clathrin machinery in neurons.
Burman JL, Wasiak S, Ritter B, de Heuvel E, McPherson PS
FEBS letters579(10)2177-84(2005 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Aftph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aftph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13906	NM_001252503.2 Latest version!	Mus musculus aftiphilin (Aftph), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu16886	NM_001290545.1 Latest version!	Mus musculus aftiphilin (Aftph), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu13915	NM_181411.4 Latest version!	Mus musculus aftiphilin (Aftph), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$531.30 $759.00
OMu45153	XM_006514625.4 Latest version!	Mus musculus aftiphilin (Aftph), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu45153	XM_006514625.3	Mus musculus aftiphilin (Aftph), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aftph ( NM_001252503.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239432.1 Mus musculus aftiphilin (Aftph), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13906
Clone ID Related Accession (Same CDS sequence)	NM_001252503.2
Accession Version	NM_001252503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	aftiphilin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645599.14, BY066578.1 and BC052036.1. On Mar 14, 2014 this sequence version replaced NM_001252503.1. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frame-shift, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK029681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGGAAACCA AAAAGAGAGG 
AGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239432.1
CDS	284..2752
Translation	MEPDIIRMYSSSPPPLDNGAEDDEEDEFGEFGGFSEVSPSGVGFVDFDTPDYTRPKEDFVPSNHFMPIHEYSEDVDSLTSFKSVQNGNDKDITAELSTPVKSQSDVVLSTTSKEMIPSKTLDPSIDGMESLEDLDKVVVQGPSTGQLRSFSPGDFRTDKNIVHQTKQLESCNGEKPPCLEILTNGFAGLETVNPQGTDDLDNVADSKGSKPLNTCGTECILESAASHATEFADFSTFSQTERTQLEEIECPVLNDGDTLTIQGNSKGPRVKELNCVKEVTLDGSFEDTGNTEREHQVCVSEIHAVADRGLSVEKQDLQTLQQDEFLNSRIQSEAWSLVDSSENSEAITKERCKMEKNDLFASKCADLSMDSVKTSDVNEIGSSKEENRKLTNPKSPDPDPTGQNALDDSAASMKNGDSGNGFVTCHDTNEDDFGDFGTANGTTPPFVTSTQDSMSDVTFEDSSEHFLHLSEPGDDFGEFEDTNAVSCQEEMRFTESDLRQTSDGLSEECPLAGESGGKDSKPDSKLKNGQDSEFGDFDSVPNTQGSAFQDSDDFADFSSAGPSQAVDWNAFEDEQKDGCSWAAFGDQQETESHHLKEVWQSQRTDETMGTLGTPKMHSVSSAASKGAVASGHLQEPGTSVQTALLNRLERIFEACFPSVFVPDVEEEVSSLKHLLETHSSPAKTREALADRGELRGVWTELQDIHDAHGLRYQWGGSHSNKKLLCSLGIDTRNILFTGNKKQPVIVPMYAAGLGMLEPTKEPLKPLSAAEKIASIGQTTVMTPEINTCTSDPFQESLPPVQFDWSSSGLTNPLDGNQKERST

Target ORF information:

RefSeq Version	NM_001252503.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus aftiphilin (Aftph), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252503.2

ORF Insert Sequence:

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGGAAACCA AAAAGAGAGG 
AGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aftph ( NM_001290545.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277474.1 Mus musculus aftiphilin (Aftph), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu16886
Clone ID Related Accession (Same CDS sequence)	NM_001290545.1
Accession Version	NM_001290545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	aftiphilin isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645599.14, BY066578.1, BC052036.1 and AK136815.1. On Mar 14, 2014 this sequence version replaced XM_006514626.1. Transcript Variant: This variant (3) contains an alternate exon in the 3' coding region, compared to variant 1. The encoded isoform (3) is longer, compared to isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGCTAGTGG AGGTTCCACT 
CTCCTGAACC TTGATTTCTT TGGGCCCGTG GATGACAGTA GCTCTAGCAG CAGCACAATC 
CCAGGTGTGG ATCCAGAGTT GTATGAGTTA ACAACTGCTA AGCTGGAGAC CTCCACCTCA 
AGCCTCAGAG TGACTGATGC ATTTGCGAAG CTCATGTCTA CAGTGGAAAA GACAAGCACG 
TCAACCAGGA AACCAAAAAG AGAGGAGCAC CTGAGTGAGG AAGCCATGAA GGTGATCGCC 
AGCCTTCCAG ACCTGACCTT CATGCATGCC AAGGTGTTGA TGTTCCCAGC CACTCTGACA 
CCTTCCATGA GCTCCCAAGA ACAAGCAGAC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277474.1
CDS	284..3079
Translation	MEPDIIRMYSSSPPPLDNGAEDDEEDEFGEFGGFSEVSPSGVGFVDFDTPDYTRPKEDFVPSNHFMPIHEYSEDVDSLTSFKSVQNGNDKDITAELSTPVKSQSDVVLSTTSKEMIPSKTLDPSIDGMESLEDLDKVVVQGPSTGQLRSFSPGDFRTDKNIVHQTKQLESCNGEKPPCLEILTNGFAGLETVNPQGTDDLDNVADSKGSKPLNTCGTECILESAASHATEFADFSTFSQTERTQLEEIECPVLNDGDTLTIQGNSKGPRVKELNCVKEVTLDGSFEDTGNTEREHQVCVSEIHAVADRGLSVEKQDLQTLQQDEFLNSRIQSEAWSLVDSSENSEAITKERCKMEKNDLFASKCADLSMDSVKTSDVNEIGSSKEENRKLTNPKSPDPDPTGQNALDDSAASMKNGDSGNGFVTCHDTNEDDFGDFGTANGTTPPFVTSTQDSMSDVTFEDSSEHFLHLSEPGDDFGEFEDTNAVSCQEEMRFTESDLRQTSDGLSEECPLAGESGGKDSKPDSKLKNGQDSEFGDFDSVPNTQGSAFQDSDDFADFSSAGPSQAVDWNAFEDEQKDGCSWAAFGDQQETESHHLKEVWQSQRTDETMGTLGTPKMHSVSSAASKGAVASGHLQEPGTSVQTALLNRLERIFEACFPSVFVPDVEEEVSSLKHLLETHSSPAKTREALADRGELRGVWTELQDIHDAHGLRYQWGGSHSNKKLLCSLGIDTRNILFTGNKKQPVIVPMYAAGLGMLEPTKEPLKPLSAAEKIASIGQTTVMTPEINTCTSDPFQESLPPVQFDWSSSGLTNPLDASGGSTLLNLDFFGPVDDSSSSSSTIPGVDPELYELTTAKLETSTSSLRVTDAFAKLMSTVEKTSTSTRKPKREEHLSEEAMKVIASLPDLTFMHAKVLMFPATLTPSMSSQEQADA

Target ORF information:

RefSeq Version	NM_001290545.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus aftiphilin (Aftph), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290545.1

ORF Insert Sequence:

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGCTAGTGG AGGTTCCACT 
CTCCTGAACC TTGATTTCTT TGGGCCCGTG GATGACAGTA GCTCTAGCAG CAGCACAATC 
CCAGGTGTGG ATCCAGAGTT GTATGAGTTA ACAACTGCTA AGCTGGAGAC CTCCACCTCA 
AGCCTCAGAG TGACTGATGC ATTTGCGAAG CTCATGTCTA CAGTGGAAAA GACAAGCACG 
TCAACCAGGA AACCAAAAAG AGAGGAGCAC CTGAGTGAGG AAGCCATGAA GGTGATCGCC 
AGCCTTCCAG ACCTGACCTT CATGCATGCC AAGGTGTTGA TGTTCCCAGC CACTCTGACA 
CCTTCCATGA GCTCCCAAGA ACAAGCAGAC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aftph ( XM_006514625.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514688.1 Mus musculus aftiphilin (Aftph), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45153
Clone ID Related Accession (Same CDS sequence)	XM_006514625.4 , XM_006514625.3
Accession Version	XM_006514625.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	aftiphilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGTGTGTGT CTTACCCCTT 
TTCACACGTA ACCCCTCCTC CCTTGGCTTA CCTTCTGTGT GGCTGCCCAC CAACACGGTC 
ACTGAATTCC AAGCTAGTGG AGGTTCCACT CTCCTGAACC TTGATTTCTT TGGGCCCGTG 
GATGACAGTA GCTCTAGCAG CAGCACAATC CCAGGTGTGG ATCCAGAGTT GTATGAGTTA 
ACAACTGCTA AGCTGGAGAC CTCCACCTCA AGCCTCAGAG TGACTGATGC ATTTGCGAAG 
CTCATGTCTA CAGTGGAAAA GACAAGCACG TCAACCAGGA AACCAAAAAG AGAGGAGCAC 
CTGAGTGAGG AAGCCATGAA GGTGATCGCC AGCCTTCCAG ACCTGACCTT CATGCATGCC 
AAGGTGTTGA TGTTCCCAGC CACTCTGACA CCTTCCATGA GCTCCCAAGA ACAAGCAGAC 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514688.1
CDS	284..3169
Translation	MEPDIIRMYSSSPPPLDNGAEDDEEDEFGEFGGFSEVSPSGVGFVDFDTPDYTRPKEDFVPSNHFMPIHEYSEDVDSLTSFKSVQNGNDKDITAELSTPVKSQSDVVLSTTSKEMIPSKTLDPSIDGMESLEDLDKVVVQGPSTGQLRSFSPGDFRTDKNIVHQTKQLESCNGEKPPCLEILTNGFAGLETVNPQGTDDLDNVADSKGSKPLNTCGTECILESAASHATEFADFSTFSQTERTQLEEIECPVLNDGDTLTIQGNSKGPRVKELNCVKEVTLDGSFEDTGNTEREHQVCVSEIHAVADRGLSVEKQDLQTLQQDEFLNSRIQSEAWSLVDSSENSEAITKERCKMEKNDLFASKCADLSMDSVKTSDVNEIGSSKEENRKLTNPKSPDPDPTGQNALDDSAASMKNGDSGNGFVTCHDTNEDDFGDFGTANGTTPPFVTSTQDSMSDVTFEDSSEHFLHLSEPGDDFGEFEDTNAVSCQEEMRFTESDLRQTSDGLSEECPLAGESGGKDSKPDSKLKNGQDSEFGDFDSVPNTQGSAFQDSDDFADFSSAGPSQAVDWNAFEDEQKDGCSWAAFGDQQETESHHLKEVWQSQRTDETMGTLGTPKMHSVSSAASKGAVASGHLQEPGTSVQTALLNRLERIFEACFPSVFVPDVEEEVSSLKHLLETHSSPAKTREALADRGELRGVWTELQDIHDAHGLRYQWGGSHSNKKLLCSLGIDTRNILFTGNKKQPVIVPMYAAGLGMLEPTKEPLKPLSAAEKIASIGQTTVMTPEINTCTSDPFQESLPPVQFDWSSSGLTNPLDVCVLPLFTRNPSSLGLPSVWLPTNTVTEFQASGGSTLLNLDFFGPVDDSSSSSSTIPGVDPELYELTTAKLETSTSSLRVTDAFAKLMSTVEKTSTSTRKPKREEHLSEEAMKVIASLPDLTFMHAKVLMFPATLTPSMSSQEQADA

Target ORF information:

RefSeq Version	XM_006514625.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aftiphilin (Aftph), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514625.3

ORF Insert Sequence:

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGTGTGTGT CTTACCCCTT 
TTCACACGTA ACCCCTCCTC CCTTGGCTTA CCTTCTGTGT GGCTGCCCAC CAACACGGTC 
ACTGAATTCC AAGCTAGTGG AGGTTCCACT CTCCTGAACC TTGATTTCTT TGGGCCCGTG 
GATGACAGTA GCTCTAGCAG CAGCACAATC CCAGGTGTGG ATCCAGAGTT GTATGAGTTA 
ACAACTGCTA AGCTGGAGAC CTCCACCTCA AGCCTCAGAG TGACTGATGC ATTTGCGAAG 
CTCATGTCTA CAGTGGAAAA GACAAGCACG TCAACCAGGA AACCAAAAAG AGAGGAGCAC 
CTGAGTGAGG AAGCCATGAA GGTGATCGCC AGCCTTCCAG ACCTGACCTT CATGCATGCC 
AAGGTGTTGA TGTTCCCAGC CACTCTGACA CCTTCCATGA GCTCCCAAGA ACAAGCAGAC 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aftph ( NM_181411.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_852076.1 Mus musculus aftiphilin (Aftph), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13915
Clone ID Related Accession (Same CDS sequence)	NM_181411.4
Accession Version	NM_181411.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	aftiphilin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645599.14, BY066578.1 and BC052036.1. On Mar 14, 2014 this sequence version replaced NM_181411.3. Transcript Variant: This variant (1) encodes isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC052036.1, AK030572.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGGTGTGGA TCCAGAGTTG 
TATGAGTTAA CAACTGCTAA GCTGGAGACC TCCACCTCAA GCCTCAGAGT GACTGATGCA 
TTTGCGAAGC TCATGTCTAC AGTGGAAAAG ACAAGCACGT CAACCAGGAA ACCAAAAAGA 
GAGGAGCACC TGAGTGAGGA AGCCATGAAG GTGATCGCCA GCCTTCCAGA CCTGACCTTC 
ATGCATGCCA AGGTGTTGAT GTTCCCAGCC ACTCTGACAC CTTCCATGAG CTCCCAAGAA 
CAAGCAGACG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852076.1
CDS	284..2998
Translation	MEPDIIRMYSSSPPPLDNGAEDDEEDEFGEFGGFSEVSPSGVGFVDFDTPDYTRPKEDFVPSNHFMPIHEYSEDVDSLTSFKSVQNGNDKDITAELSTPVKSQSDVVLSTTSKEMIPSKTLDPSIDGMESLEDLDKVVVQGPSTGQLRSFSPGDFRTDKNIVHQTKQLESCNGEKPPCLEILTNGFAGLETVNPQGTDDLDNVADSKGSKPLNTCGTECILESAASHATEFADFSTFSQTERTQLEEIECPVLNDGDTLTIQGNSKGPRVKELNCVKEVTLDGSFEDTGNTEREHQVCVSEIHAVADRGLSVEKQDLQTLQQDEFLNSRIQSEAWSLVDSSENSEAITKERCKMEKNDLFASKCADLSMDSVKTSDVNEIGSSKEENRKLTNPKSPDPDPTGQNALDDSAASMKNGDSGNGFVTCHDTNEDDFGDFGTANGTTPPFVTSTQDSMSDVTFEDSSEHFLHLSEPGDDFGEFEDTNAVSCQEEMRFTESDLRQTSDGLSEECPLAGESGGKDSKPDSKLKNGQDSEFGDFDSVPNTQGSAFQDSDDFADFSSAGPSQAVDWNAFEDEQKDGCSWAAFGDQQETESHHLKEVWQSQRTDETMGTLGTPKMHSVSSAASKGAVASGHLQEPGTSVQTALLNRLERIFEACFPSVFVPDVEEEVSSLKHLLETHSSPAKTREALADRGELRGVWTELQDIHDAHGLRYQWGGSHSNKKLLCSLGIDTRNILFTGNKKQPVIVPMYAAGLGMLEPTKEPLKPLSAAEKIASIGQTTVMTPEINTCTSDPFQESLPPVQFDWSSSGLTNPLDGVDPELYELTTAKLETSTSSLRVTDAFAKLMSTVEKTSTSTRKPKREEHLSEEAMKVIASLPDLTFMHAKVLMFPATLTPSMSSQEQADA

Target ORF information:

RefSeq Version	NM_181411.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus aftiphilin (Aftph), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181411.4

ORF Insert Sequence:

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGGTGTGGA TCCAGAGTTG 
TATGAGTTAA CAACTGCTAA GCTGGAGACC TCCACCTCAA GCCTCAGAGT GACTGATGCA 
TTTGCGAAGC TCATGTCTAC AGTGGAAAAG ACAAGCACGT CAACCAGGAA ACCAAAAAGA 
GAGGAGCACC TGAGTGAGGA AGCCATGAAG GTGATCGCCA GCCTTCCAGA CCTGACCTTC 
ATGCATGCCA AGGTGTTGAT GTTCCCAGCC ACTCTGACAC CTTCCATGAG CTCCCAAGAA 
CAAGCAGACG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aftph ( XM_006514625.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514688.1 Mus musculus aftiphilin (Aftph), transcript variant X1, mRNA.

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CloneID	OMu45153
Clone ID Related Accession (Same CDS sequence)	XM_006514625.4 , XM_006514625.3
Accession Version	XM_006514625.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	aftiphilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514625.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGTGTGTGT CTTACCCCTT 
TTCACACGTA ACCCCTCCTC CCTTGGCTTA CCTTCTGTGT GGCTGCCCAC CAACACGGTC 
ACTGAATTCC AAGCTAGTGG AGGTTCCACT CTCCTGAACC TTGATTTCTT TGGGCCCGTG 
GATGACAGTA GCTCTAGCAG CAGCACAATC CCAGGTGTGG ATCCAGAGTT GTATGAGTTA 
ACAACTGCTA AGCTGGAGAC CTCCACCTCA AGCCTCAGAG TGACTGATGC ATTTGCGAAG 
CTCATGTCTA CAGTGGAAAA GACAAGCACG TCAACCAGGA AACCAAAAAG AGAGGAGCAC 
CTGAGTGAGG AAGCCATGAA GGTGATCGCC AGCCTTCCAG ACCTGACCTT CATGCATGCC 
AAGGTGTTGA TGTTCCCAGC CACTCTGACA CCTTCCATGA GCTCCCAAGA ACAAGCAGAC 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514688.1
CDS	323..3208
Translation	MEPDIIRMYSSSPPPLDNGAEDDEEDEFGEFGGFSEVSPSGVGFVDFDTPDYTRPKEDFVPSNHFMPIHEYSEDVDSLTSFKSVQNGNDKDITAELSTPVKSQSDVVLSTTSKEMIPSKTLDPSIDGMESLEDLDKVVVQGPSTGQLRSFSPGDFRTDKNIVHQTKQLESCNGEKPPCLEILTNGFAGLETVNPQGTDDLDNVADSKGSKPLNTCGTECILESAASHATEFADFSTFSQTERTQLEEIECPVLNDGDTLTIQGNSKGPRVKELNCVKEVTLDGSFEDTGNTEREHQVCVSEIHAVADRGLSVEKQDLQTLQQDEFLNSRIQSEAWSLVDSSENSEAITKERCKMEKNDLFASKCADLSMDSVKTSDVNEIGSSKEENRKLTNPKSPDPDPTGQNALDDSAASMKNGDSGNGFVTCHDTNEDDFGDFGTANGTTPPFVTSTQDSMSDVTFEDSSEHFLHLSEPGDDFGEFEDTNAVSCQEEMRFTESDLRQTSDGLSEECPLAGESGGKDSKPDSKLKNGQDSEFGDFDSVPNTQGSAFQDSDDFADFSSAGPSQAVDWNAFEDEQKDGCSWAAFGDQQETESHHLKEVWQSQRTDETMGTLGTPKMHSVSSAASKGAVASGHLQEPGTSVQTALLNRLERIFEACFPSVFVPDVEEEVSSLKHLLETHSSPAKTREALADRGELRGVWTELQDIHDAHGLRYQWGGSHSNKKLLCSLGIDTRNILFTGNKKQPVIVPMYAAGLGMLEPTKEPLKPLSAAEKIASIGQTTVMTPEINTCTSDPFQESLPPVQFDWSSSGLTNPLDVCVLPLFTRNPSSLGLPSVWLPTNTVTEFQASGGSTLLNLDFFGPVDDSSSSSSTIPGVDPELYELTTAKLETSTSSLRVTDAFAKLMSTVEKTSTSTRKPKREEHLSEEAMKVIASLPDLTFMHAKVLMFPATLTPSMSSQEQADA

Target ORF information:

RefSeq Version	XM_006514625.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus aftiphilin (Aftph), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514625.4

ORF Insert Sequence:

ATGGAGCCAG ACATCATTCG AATGTACTCT TCCTCCCCCC CACCTCTAGA CAACGGAGCA 
GAGGATGATG AGGAGGATGA GTTTGGGGAG TTTGGTGGGT TTTCGGAAGT CAGCCCTTCT 
GGTGTAGGGT TTGTTGATTT TGACACACCA GATTATACTC GTCCCAAGGA AGACTTTGTA 
CCTTCAAACC ACTTTATGCC AATTCATGAA TATTCAGAAG ATGTAGATAG CCTTACAAGC 
TTTAAGTCTG TTCAGAATGG TAATGATAAG GATATCACTG CTGAACTTTC TACTCCTGTG 
AAAAGTCAAT CTGATGTTGT ACTTTCTACC ACCAGCAAAG AAATGATTCC ATCTAAAACT 
TTGGATCCTT CCATCGATGG TATGGAAAGC CTGGAAGACT TAGATAAAGT AGTGGTGCAG 
GGACCAAGCA CTGGGCAACT CAGAAGTTTC TCTCCTGGAG ATTTTAGAAC TGATAAGAAC 
ATTGTTCACC AAACCAAGCA GTTAGAGAGC TGCAACGGTG AAAAGCCTCC TTGTCTGGAA 
ATTCTAACAA ACGGGTTTGC AGGTTTGGAA ACTGTAAATC CTCAGGGAAC AGATGACCTG 
GACAATGTGG CAGATTCAAA GGGATCGAAG CCTCTTAACA CTTGTGGCAC CGAGTGCATT 
TTAGAGTCTG CAGCTAGTCA TGCTACGGAA TTTGCAGATT TTTCTACATT TTCCCAAACA 
GAAAGGACAC AACTGGAAGA AATAGAATGT CCAGTTTTAA ATGATGGTGA TACACTGACC 
ATTCAGGGAA ACAGCAAAGG CCCCAGAGTC AAGGAACTAA ACTGTGTGAA AGAAGTGACT 
TTGGATGGAA GCTTTGAGGA TACGGGGAAC ACTGAAAGAG AACATCAGGT CTGCGTCTCA 
GAAATCCATG CCGTAGCTGA CAGAGGGCTC AGTGTTGAAA AACAAGACCT TCAAACACTG 
CAACAGGATG AGTTTCTAAA TTCAAGGATT CAGTCTGAGG CTTGGAGCTT GGTAGACTCA 
AGTGAAAATT CAGAAGCCAT TACAAAGGAG CGGTGTAAAA TGGAAAAAAA TGACTTATTT 
GCCTCTAAGT GTGCTGACTT ATCCATGGAT TCTGTTAAAA CTTCAGACGT TAATGAAATT 
GGTTCTTCCA AAGAAGAAAA CAGAAAGCTG ACTAATCCCA AAAGCCCAGA CCCTGATCCC 
ACAGGACAAA ATGCTTTGGA TGATTCTGCT GCAAGCATGA AAAATGGTGA CAGTGGGAAT 
GGCTTTGTGA CTTGTCATGA TACCAATGAG GATGATTTTG GTGACTTTGG TACAGCCAAT 
GGCACCACTC CACCCTTTGT TACTAGTACT CAAGATTCAA TGAGTGACGT CACTTTTGAA 
GACTCCTCAG AGCATTTTCT ACATCTTAGT GAACCAGGTG ATGATTTTGG AGAATTTGAG 
GATACAAATG CTGTTTCTTG TCAAGAGGAA ATGAGATTTA CTGAGTCAGA CCTAAGACAG 
ACTTCTGATG GCTTATCAGA GGAATGTCCA TTGGCAGGAG AGTCTGGTGG AAAAGACAGT 
AAACCTGACT CAAAACTGAA AAATGGTCAA GACAGTGAAT TTGGAGATTT TGATTCTGTG 
CCAAATACTC AAGGTAGTGC TTTTCAAGAC TCGGATGATT TTGCAGACTT CAGTTCAGCA 
GGTCCTAGCC AAGCTGTAGA TTGGAATGCT TTTGAGGATG AACAGAAAGA TGGTTGTTCT 
TGGGCTGCCT TTGGGGATCA GCAGGAGACT GAGTCTCATC ATCTAAAGGA AGTCTGGCAG 
TCACAGAGGA CAGATGAAAC TATGGGCACT CTGGGAACCC CTAAGATGCA CAGTGTTTCT 
TCAGCAGCTT CCAAAGGAGC AGTTGCTAGT GGCCATTTGC AGGAACCAGG CACTTCAGTT 
CAGACAGCTT TACTCAACCG GTTGGAGAGA ATTTTCGAAG CCTGTTTTCC GTCTGTGTTT 
GTCCCTGATG TGGAAGAGGA GGTTTCTTCC CTGAAGCACT TGCTGGAGAC ACACAGCTCG 
CCAGCAAAAA CAAGAGAGGC CTTAGCTGAC CGTGGTGAGT TGAGGGGTGT ATGGACCGAA 
CTGCAGGACA TCCATGATGC ACATGGTTTG CGGTACCAGT GGGGTGGCTC ACATAGCAAC 
AAGAAGCTTT TGTGCTCCTT GGGAATAGAC ACTCGAAACA TTCTATTCAC TGGCAATAAG 
AAGCAACCTG TTATAGTGCC CATGTATGCA GCAGGATTGG GAATGTTAGA GCCCACCAAG 
GAACCATTAA AACCACTATC TGCTGCAGAA AAAATTGCTT CTATTGGTCA GACAACCGTT 
ATGACACCAG AAATAAACAC GTGTACATCT GATCCATTCC AGGAGTCTCT CCCACCTGTC 
CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG ATGTGTGTGT CTTACCCCTT 
TTCACACGTA ACCCCTCCTC CCTTGGCTTA CCTTCTGTGT GGCTGCCCAC CAACACGGTC 
ACTGAATTCC AAGCTAGTGG AGGTTCCACT CTCCTGAACC TTGATTTCTT TGGGCCCGTG 
GATGACAGTA GCTCTAGCAG CAGCACAATC CCAGGTGTGG ATCCAGAGTT GTATGAGTTA 
ACAACTGCTA AGCTGGAGAC CTCCACCTCA AGCCTCAGAG TGACTGATGC ATTTGCGAAG 
CTCATGTCTA CAGTGGAAAA GACAAGCACG TCAACCAGGA AACCAAAAAG AGAGGAGCAC 
CTGAGTGAGG AAGCCATGAA GGTGATCGCC AGCCTTCCAG ACCTGACCTT CATGCATGCC 
AAGGTGTTGA TGTTCCCAGC CACTCTGACA CCTTCCATGA GCTCCCAAGA ACAAGCAGAC 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Aftiphilin is a component of the clathrin machinery in neurons.
FEBS letters579(10)2177-84(2005 Apr)
Burman JL,Wasiak S,Ritter B,de Heuvel E,McPherson PS

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Aftph cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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