Tspan8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tspan8
Entrez Gene ID	216350
Full Name	tetraspanin 8
Synonyms	C76990,E330007O21Rik,Tm4sf3
General protein information	Preferred Names tetraspanin 8 Names tetraspanin-8 transmembrane 4 superfamily member 3 tspan-8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 D2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006513542.1	XP_006513605.1	tetraspanin-8 isoform X1
NM_001168680.1	NP_001162151.1	tetraspanin-8
NM_001168679.1	NP_001162150.1	tetraspanin-8
XM_006513543.4	XP_006513606.1	tetraspanin-8 isoform X2
NM_146010.2	NP_666122.1	tetraspanin-8
XM_006513542.2	XP_006513605.1	tetraspanin-8 isoform X1
XM_006513543.3	XP_006513606.1	tetraspanin-8 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TSPAN8	XP_531678.2
Gallus gallus (chicken)	TSPAN8	NP_001186579.1
Xenopus tropicalis (tropical clawed frog)	tspan8	NP_001011430.2
Bos taurus (cattle)	TSPAN8	NP_001073097.1
Mus musculus (house mouse)	Tspan8	NP_666122.1
Rattus norvegicus (Norway rat)	Tspan8	NP_598210.1
Homo sapiens (human)	TSPAN8	NP_004607.1
Pan troglodytes (chimpanzee)	TSPAN8	XP_001157562.1
	TSPAN8	XP_001117478.2

Gene Ontology
Bibliography

Related articles in PubMed

The regulation of tetraspanin 8 gene expression-A potential new mechanism in the pathogenesis of bipolar disorder.
Schartner C, Scholz CJ, Weber H, Post A, Freudenberg F, Gr?newald L, Reif A
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics174(7)740-750(2017 Oct)

TSPAN8 Expression Distinguishes Spermatogonial Stem Cells in the Prepubertal Mouse Testis.
Mutoji K, Singh A, Nguyen T, Gildersleeve H, Kaucher AV, Oatley MJ, Oatley JM, Velte EK, Geyer CB, Cheng K, McCarrey JR, Hermann BP
Biology of reproduction95(6)117(2016 12)

Reduced body weight in male Tspan8-deficient mice.
Champy MF, Le Voci L, Selloum M, Peterson LB, Cumiskey AM, Blom D
International journal of obesity (2005)35(4)605-17(2011 Apr)

Alterations in gene expression in MEN1-associated insulinoma development.
Serewko-Auret MM, Mould AW, Loffler KA, Duncan R, Kay GF, Hayward NK
Pancreas39(8)1140-6(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transplantation studies provided definitive evidence that a TSPAN8-high subpopulation is enriched for spermatogonial stem cells.
Title: TSPAN8 Expression Distinguishes Spermatogonial Stem Cells in the Prepubertal Mouse Testis.

results argue for a role for Tspan8 in body-weight regulation in males, but do not show differences in T2D-associated traits that were anticipated from previous human genome-wide association studies.
Title: Reduced body weight in male Tspan8-deficient mice.

Results suggest that alterations in gene expression of Gata6, Tspan8, S100a8, and Lmo2 may act via novel pathways that play functionally important roles in Men1-associated tumor progression.
Title: Alterations in gene expression in MEN1-associated insulinoma development.

The following Tspan8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tspan8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11571	NM_001168680.1 Latest version!	Mus musculus tetraspanin 8 (Tspan8), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu11571	NM_001168679.1 Latest version!	Mus musculus tetraspanin 8 (Tspan8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu11571	NM_146010.2 Latest version!	Mus musculus tetraspanin 8 (Tspan8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu11571	XM_006513542.2 Latest version!	Mus musculus tetraspanin 8 (Tspan8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu44427	XM_006513543.4 Latest version!	Mus musculus tetraspanin 8 (Tspan8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu11571	XM_006513542.1	Mus musculus tetraspanin 8 (Tspan8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu44427	XM_006513543.3	Mus musculus tetraspanin 8 (Tspan8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tspan8 ( XM_006513543.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513606.1 Mus musculus tetraspanin 8 (Tspan8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44427
Clone ID Related Accession (Same CDS sequence)	XM_006513543.4 , XM_006513543.3
Accession Version	XM_006513543.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tetraspanin-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTAG GTTTCCTGGG ATGCTGTGGA GCTGTGAAAG AAAGCCGGTG CATGCTTCTC 
TTGTTTTTTA TCGGCTTGCT GCTGATTCTG ATTCTGCAAG TGGCAGCAGG CATCCTAGGA 
GCCGCTTTCA AACCTGAGTA TAACCGAATT CTGAATGAAA CGCTATATGA AAATGCTAAG 
CTTCTGTCGG ACAACACTGA TGAGGCAAAA GACTTCCAGA AGGCCATGAT TGTGTTTCAG 
TCGGAGTTCA AGTGCTGTGG CTTGGAGAAT GGAGCTGCCG ACTGGGGAAA TAATTTTGTG 
GAGGCAAAAG AGTCATGTCA ATGTACAGGA ACTGACTGTG CAACTTATCA GGGGAGTTCC 
GTTTACCCAA AGACCTGTCT TTCTTTGATC AAAGATCTGT TTGAAAAGAA CATTATTATT 
GTCATTGGAA TTGCTTTTGG ACTGGCCGTT ATTGAGATTC TCGGTTTGGT GTTTTCTATG 
GTGCTATACT GCCAGATTGG AAGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513606.1
CDS	190..699
Translation	MVLGFLGCCGAVKESRCMLLLFFIGLLLILILQVAAGILGAAFKPEYNRILNETLYENAKLLSDNTDEAKDFQKAMIVFQSEFKCCGLENGAADWGNNFVEAKESCQCTGTDCATYQGSSVYPKTCLSLIKDLFEKNIIIVIGIAFGLAVIEILGLVFSMVLYCQIGSK

Target ORF information:

RefSeq Version	XM_006513543.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetraspanin 8 (Tspan8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513543.3

ORF Insert Sequence:

ATGGTTCTAG GTTTCCTGGG ATGCTGTGGA GCTGTGAAAG AAAGCCGGTG CATGCTTCTC 
TTGTTTTTTA TCGGCTTGCT GCTGATTCTG ATTCTGCAAG TGGCAGCAGG CATCCTAGGA 
GCCGCTTTCA AACCTGAGTA TAACCGAATT CTGAATGAAA CGCTATATGA AAATGCTAAG 
CTTCTGTCGG ACAACACTGA TGAGGCAAAA GACTTCCAGA AGGCCATGAT TGTGTTTCAG 
TCGGAGTTCA AGTGCTGTGG CTTGGAGAAT GGAGCTGCCG ACTGGGGAAA TAATTTTGTG 
GAGGCAAAAG AGTCATGTCA ATGTACAGGA ACTGACTGTG CAACTTATCA GGGGAGTTCC 
GTTTACCCAA AGACCTGTCT TTCTTTGATC AAAGATCTGT TTGAAAAGAA CATTATTATT 
GTCATTGGAA TTGCTTTTGG ACTGGCCGTT ATTGAGATTC TCGGTTTGGT GTTTTCTATG 
GTGCTATACT GCCAGATTGG AAGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tspan8 ( NM_001168680.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001162151.1 Mus musculus tetraspanin 8 (Tspan8), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu11571
Clone ID Related Accession (Same CDS sequence)	XM_006513542.1 , NM_001168680.1 , NM_001168679.1 , NM_146010.2 , XM_006513542.2
Accession Version	NM_001168680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	tetraspanin-8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077301.1, DV060937.1 and AC153364.4. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK077301.1, BC029068.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162151.1
CDS	212..919
Translation	MAGVSSCLKYSMFFFNFLFWVCGTLILGLAIWVRVSKDGKEIITSGDSSTNPFIAVNILIAVGSIIMVLGFLGCCGAVKESRCMLLLFFIGLLLILILQVAAGILGAAFKPEYNRILNETLYENAKLLSDNTDEAKDFQKAMIVFQSEFKCCGLENGAADWGNNFVEAKESCQCTGTDCATYQGSSVYPKTCLSLIKDLFEKNIIIVIGIAFGLAVIEILGLVFSMVLYCQIGSK

Target ORF information:

RefSeq Version	NM_001168680.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetraspanin 8 (Tspan8), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168680.1

ORF Insert Sequence:

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tspan8 ( NM_001168679.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001162150.1 Mus musculus tetraspanin 8 (Tspan8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11571
Clone ID Related Accession (Same CDS sequence)	XM_006513542.1 , NM_001168680.1 , NM_001168679.1 , NM_146010.2 , XM_006513542.2
Accession Version	NM_001168679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	tetraspanin-8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV065030.1, DV060937.1, AK077301.1 and AC153364.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC027777.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162150.1
CDS	176..883
Translation	MAGVSSCLKYSMFFFNFLFWVCGTLILGLAIWVRVSKDGKEIITSGDSSTNPFIAVNILIAVGSIIMVLGFLGCCGAVKESRCMLLLFFIGLLLILILQVAAGILGAAFKPEYNRILNETLYENAKLLSDNTDEAKDFQKAMIVFQSEFKCCGLENGAADWGNNFVEAKESCQCTGTDCATYQGSSVYPKTCLSLIKDLFEKNIIIVIGIAFGLAVIEILGLVFSMVLYCQIGSK

Target ORF information:

RefSeq Version	NM_001168679.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetraspanin 8 (Tspan8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168679.1

ORF Insert Sequence:

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tspan8 ( NM_146010.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666122.1 Mus musculus tetraspanin 8 (Tspan8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11571
Clone ID Related Accession (Same CDS sequence)	XM_006513542.1 , NM_001168680.1 , NM_001168679.1 , NM_146010.2 , XM_006513542.2
Accession Version	NM_146010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	tetraspanin-8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126270.4, DV060937.1, AK077301.1 and AC153364.4. On Dec 12, 2009 this sequence version replaced NM_146010.1. Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC029808.1, BC034198.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666122.1
CDS	318..1025
Translation	MAGVSSCLKYSMFFFNFLFWVCGTLILGLAIWVRVSKDGKEIITSGDSSTNPFIAVNILIAVGSIIMVLGFLGCCGAVKESRCMLLLFFIGLLLILILQVAAGILGAAFKPEYNRILNETLYENAKLLSDNTDEAKDFQKAMIVFQSEFKCCGLENGAADWGNNFVEAKESCQCTGTDCATYQGSSVYPKTCLSLIKDLFEKNIIIVIGIAFGLAVIEILGLVFSMVLYCQIGSK

Target ORF information:

RefSeq Version	NM_146010.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetraspanin 8 (Tspan8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146010.2

ORF Insert Sequence:

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tspan8 ( XM_006513542.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513605.1 Mus musculus tetraspanin 8 (Tspan8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu11571
Clone ID Related Accession (Same CDS sequence)	XM_006513542.1 , NM_001168680.1 , NM_001168679.1 , NM_146010.2 , XM_006513542.2
Accession Version	XM_006513542.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	tetraspanin-8 isoform X1
Comment

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513605.1
Translation	MAGVSSCLKYSMFFFNFLFWVCGTLILGLAIWVRVSKDGKEIITSGDSSTNPFIAVNILIAVGSIIMVLGFLGCCGAVKESRCMLLLFFIGLLLILILQVAAGILGAAFKPEYNRILNETLYENAKLLSDNTDEAKDFQKAMIVFQSEFKCCGLENGAADWGNNFVEAKESCQCTGTDCATYQGSSVYPKTCLSLIKDLFEKNIIIVIGIAFGLAVIEILGLVFSMVLYCQIGSK

Target ORF information:

RefSeq Version	XM_006513542.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus tetraspanin 8 (Tspan8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513542.1

ORF Insert Sequence:

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tspan8 ( XM_006513542.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513605.1 Mus musculus tetraspanin 8 (Tspan8), transcript variant X1, mRNA.

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CloneID	OMu11571
Clone ID Related Accession (Same CDS sequence)	XM_006513542.1 , NM_001168680.1 , NM_001168679.1 , NM_146010.2 , XM_006513542.2
Accession Version	XM_006513542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tetraspanin-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513605.1
CDS	291..998
Translation	MAGVSSCLKYSMFFFNFLFWVCGTLILGLAIWVRVSKDGKEIITSGDSSTNPFIAVNILIAVGSIIMVLGFLGCCGAVKESRCMLLLFFIGLLLILILQVAAGILGAAFKPEYNRILNETLYENAKLLSDNTDEAKDFQKAMIVFQSEFKCCGLENGAADWGNNFVEAKESCQCTGTDCATYQGSSVYPKTCLSLIKDLFEKNIIIVIGIAFGLAVIEILGLVFSMVLYCQIGSK

Target ORF information:

RefSeq Version	XM_006513542.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetraspanin 8 (Tspan8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513542.2

ORF Insert Sequence:

ATGGCAGGTG TGAGCAGCTG TTTAAAGTAT TCTATGTTTT TCTTCAACTT CTTGTTCTGG 
GTATGTGGTA CACTGATCTT GGGACTGGCC ATATGGGTGA GAGTAAGCAA AGATGGAAAA 
GAGATCATCA CGTCAGGAGA CTCTAGCACG AACCCCTTCA TTGCTGTGAA TATCTTGATT 
GCAGTTGGGT CTATCATCAT GGTTCTAGGT TTCCTGGGAT GCTGTGGAGC TGTGAAAGAA 
AGCCGGTGCA TGCTTCTCTT GTTTTTTATC GGCTTGCTGC TGATTCTGAT TCTGCAAGTG 
GCAGCAGGCA TCCTAGGAGC CGCTTTCAAA CCTGAGTATA ACCGAATTCT GAATGAAACG 
CTATATGAAA ATGCTAAGCT TCTGTCGGAC AACACTGATG AGGCAAAAGA CTTCCAGAAG 
GCCATGATTG TGTTTCAGTC GGAGTTCAAG TGCTGTGGCT TGGAGAATGG AGCTGCCGAC 
TGGGGAAATA ATTTTGTGGA GGCAAAAGAG TCATGTCAAT GTACAGGAAC TGACTGTGCA 
ACTTATCAGG GGAGTTCCGT TTACCCAAAG ACCTGTCTTT CTTTGATCAA AGATCTGTTT 
GAAAAGAACA TTATTATTGT CATTGGAATT GCTTTTGGAC TGGCCGTTAT TGAGATTCTC 
GGTTTGGTGT TTTCTATGGT GCTATACTGC CAGATTGGAA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tspan8 ( XM_006513543.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513606.1 Mus musculus tetraspanin 8 (Tspan8), transcript variant X2, mRNA.

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CloneID	OMu44427
Clone ID Related Accession (Same CDS sequence)	XM_006513543.4 , XM_006513543.3
Accession Version	XM_006513543.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tetraspanin-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513543.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTAG GTTTCCTGGG ATGCTGTGGA GCTGTGAAAG AAAGCCGGTG CATGCTTCTC 
TTGTTTTTTA TCGGCTTGCT GCTGATTCTG ATTCTGCAAG TGGCAGCAGG CATCCTAGGA 
GCCGCTTTCA AACCTGAGTA TAACCGAATT CTGAATGAAA CGCTATATGA AAATGCTAAG 
CTTCTGTCGG ACAACACTGA TGAGGCAAAA GACTTCCAGA AGGCCATGAT TGTGTTTCAG 
TCGGAGTTCA AGTGCTGTGG CTTGGAGAAT GGAGCTGCCG ACTGGGGAAA TAATTTTGTG 
GAGGCAAAAG AGTCATGTCA ATGTACAGGA ACTGACTGTG CAACTTATCA GGGGAGTTCC 
GTTTACCCAA AGACCTGTCT TTCTTTGATC AAAGATCTGT TTGAAAAGAA CATTATTATT 
GTCATTGGAA TTGCTTTTGG ACTGGCCGTT ATTGAGATTC TCGGTTTGGT GTTTTCTATG 
GTGCTATACT GCCAGATTGG AAGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513606.1
CDS	692..1201
Translation	MVLGFLGCCGAVKESRCMLLLFFIGLLLILILQVAAGILGAAFKPEYNRILNETLYENAKLLSDNTDEAKDFQKAMIVFQSEFKCCGLENGAADWGNNFVEAKESCQCTGTDCATYQGSSVYPKTCLSLIKDLFEKNIIIVIGIAFGLAVIEILGLVFSMVLYCQIGSK

Target ORF information:

RefSeq Version	XM_006513543.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetraspanin 8 (Tspan8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513543.4

ORF Insert Sequence:

ATGGTTCTAG GTTTCCTGGG ATGCTGTGGA GCTGTGAAAG AAAGCCGGTG CATGCTTCTC 
TTGTTTTTTA TCGGCTTGCT GCTGATTCTG ATTCTGCAAG TGGCAGCAGG CATCCTAGGA 
GCCGCTTTCA AACCTGAGTA TAACCGAATT CTGAATGAAA CGCTATATGA AAATGCTAAG 
CTTCTGTCGG ACAACACTGA TGAGGCAAAA GACTTCCAGA AGGCCATGAT TGTGTTTCAG 
TCGGAGTTCA AGTGCTGTGG CTTGGAGAAT GGAGCTGCCG ACTGGGGAAA TAATTTTGTG 
GAGGCAAAAG AGTCATGTCA ATGTACAGGA ACTGACTGTG CAACTTATCA GGGGAGTTCC 
GTTTACCCAA AGACCTGTCT TTCTTTGATC AAAGATCTGT TTGAAAAGAA CATTATTATT 
GTCATTGGAA TTGCTTTTGG ACTGGCCGTT ATTGAGATTC TCGGTTTGGT GTTTTCTATG 
GTGCTATACT GCCAGATTGG AAGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The regulation of tetraspanin 8 gene expression-A potential new mechanism in the pathogenesis of bipolar disorder.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics174(7)740-750(2017 Oct)
Schartner C,Scholz CJ,Weber H,Post A,Freudenberg F,Gr?newald L,Reif A

TSPAN8 Expression Distinguishes Spermatogonial Stem Cells in the Prepubertal Mouse Testis.
Biology of reproduction95(6)117(2016 12)
Mutoji K,Singh A,Nguyen T,Gildersleeve H,Kaucher AV,Oatley MJ,Oatley JM,Velte EK,Geyer CB,Cheng K,McCarrey JR,Hermann BP

Reduced body weight in male Tspan8-deficient mice.
International journal of obesity (2005)35(4)605-17(2011 Apr)
Champy MF,Le Voci L,Selloum M,Peterson LB,Cumiskey AM,Blom D

Alterations in gene expression in MEN1-associated insulinoma development.
Pancreas39(8)1140-6(2010 Nov)
Serewko-Auret MM,Mould AW,Loffler KA,Duncan R,Kay GF,Hayward NK

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Tspan8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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