Slc5a8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc5a8
Entrez Gene ID	216225
Full Name	solute carrier family 5 (iodide transporter), member 8
Synonyms	Ait,SMCT
General protein information	Preferred Names solute carrier family 5 (iodide transporter), member 8 Names sodium-coupled monocarboxylate transporter 1 electrogenic sodium monocarboxylate cotransporter slc5a8 solute carrier family 5 member 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_145423.2	NP_663398.2	sodium-coupled monocarboxylate transporter 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC5A8	NP_666018.3
	SLC5A8	XP_001091485.2
Canis lupus familiaris (dog)	SLC5A8	XP_539743.3
Bos taurus (cattle)	SLC5A8	NP_001158333.1
Pan troglodytes (chimpanzee)	SLC5A8	XP_522508.2
Rattus norvegicus (Norway rat)	Slc5a8	NP_001178916.1
Gallus gallus (chicken)	SLC5A8	XP_416173.3
Mus musculus (house mouse)	Slc5a8	NP_663398.2
Danio rerio (zebrafish)	slc5a8l	NP_001030141.1
Drosophila melanogaster (fruit fly)	CG32669	NP_727460.1
Xenopus tropicalis (tropical clawed frog)	slc5a8	NP_988910.2

Gene Ontology
Bibliography

Related articles in PubMed

SLC transporters as a novel class of tumour suppressors: identity, function and molecular mechanisms.
Bhutia YD, Babu E, Ramachandran S, Yang S, Thangaraju M, Ganapathy V
The Biochemical journal473(9)1113-24(2016 05)

Slc5a8, a Na+-coupled high-affinity transporter for short-chain fatty acids, is a conditional tumour suppressor in colon that protects against colitis and colon cancer under low-fibre dietary conditions.
Gurav A, Sivaprakasam S, Bhutia YD, Boettger T, Singh N, Ganapathy V
The Biochemical journal469(2)267-78(2015 Jul)

Molecular mechanism of SLC5A8 inactivation in breast cancer.
Elangovan S, Pathania R, Ramachandran S, Ananth S, Padia RN, Srinivas SR, Babu E, Hawthorn L, Schoenlein PV, Boettger T, Smith SB, Prasad PD, Ganapathy V, Thangaraju M
Molecular and cellular biology33(19)3920-35(2013 Oct)

Induction of the cystine/glutamate exchanger SLC7A11 in retinal pigment epithelial cells by the antipsoriatic drug monomethylfumarate.
Ananth S, Babu E, Veeranan-Karmegam R, Bozard Baldowski BR, Boettger T, Martin PM
Investigative ophthalmology & visual science54(3)1592-602(2013 Mar)

Transport via SLC5A8 (SMCT1) is obligatory for 2-oxothiazolidine-4-carboxylate to enhance glutathione production in retinal pigment epithelial cells.
Babu E, Ananth S, Veeranan-Karmegam R, Coothankandaswamy V, Smith SB, Boettger T, Ganapathy V, Martin PM
Investigative ophthalmology & visual science52(8)5749-57(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Slc5a8 is an obligatory link between dietary fibre and mucosal immune system via the bacterial metabolite butyrate and that this transporter is a conditional tumour suppressor in colon linked to dietary fibre content.
Title: Slc5a8, a Na+-coupled high-affinity transporter for short-chain fatty acids, is a conditional tumour suppressor in colon that protects against colitis and colon cancer under low-fibre dietary conditions.

Data indicate oncogenic HRAS (HRAS(G12V)) as a potent mediator of SLC5A8 silencing in human nontransformed normal mammary epithelial cell lines and in mouse mammary tumors through DNMT1.
Title: Molecular mechanism of SLC5A8 inactivation in breast cancer.

Efficacy of LP or their secreted soluble factors to stimulate SMCT1 expression and function and to counteract the inhibitory effects of TNF-alpha on butyrate absorption could have potential therapeutic value.
Title: The probiotic Lactobacillus plantarum counteracts TNF-{alpha}-induced downregulation of SMCT1 expression and function.

butyrate and propionate block the generation of dendritic cells from bone marrow stem cells, since Slc5a8 transports butyrate and propionate into cells, and these two bacterial metabolites inhibit histone deacetylases
Title: Blockade of dendritic cell development by bacterial fermentation products butyrate and propionate through a transporter (Slc5a8)-dependent inhibition of histone deacetylases.

Sodium-coupled electrogenic transport of pyroglutamate (5-oxoproline) via SLC5A8, a monocarboxylate transporter.
Title: Sodium-coupled electrogenic transport of pyroglutamate (5-oxoproline) via SLC5A8, a monocarboxylate transporter.

The following Slc5a8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc5a8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18623	NM_145423.2 Latest version!	Mus musculus solute carrier family 5 (iodide transporter), member 8 (Slc5a8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc5a8 ( NM_145423.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663398.2 Mus musculus solute carrier family 5 (iodide transporter), member 8 (Slc5a8), mRNA.

Price & Availability↑

CloneID	OMu18623
Clone ID Related Accession (Same CDS sequence)	NM_145423.2
Accession Version	NM_145423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	sodium-coupled monocarboxylate transporter 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY484428.1. On Jul 14, 2006 this sequence version replaced NM_145423.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY484428.1, BC017691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGT CGCGGGACAT CGGCAGTTTT GTGGTGTGGG ACTACGTGGT GTTCGCAGGC 
ATGCTGCTCA TCTCGGCGGC TATAGGCATC TATTACGCCT TCGCGGGGGG CGGCCAGCAG 
ACCTCCAAGG ACTTTCTTAT GGGCGGTCGC AGTATGTCCG CCGTGCCTGT GGCTCTGTCC 
CTCACCGCCA GCTTCATGTC CGCTGTCACT GTCCTGGGCA CTCCTGCCGA GGTCTACCGT 
TTTGGGGCGA TATTCAGCAT CTTTGTCATC ACCTACTTTT TTGTAGTGGT TATCAGCGCG 
GAGGTCTTCC TTCCAGTGTT CTATAGGCTG GGGATCACCA GCACCTACGA GTATTTGGAA 
CTTCGATTTA ATCGATGCAT TCGTCTCTGT GGCACAATCC TCTTCATCGT TCAAACAATT 
CTGTACACTG GAATTGTGAT TTATGCCCCT GCTCTGGCTT TGAATCAAGT TACAGGATTT 
GATCTGTGGG GCGCAGTGGT GGCCACAGGG GTGGTCTGCA CATTCTACTG CACACTGGGC 
GGTCTTAAAG CAGTTGTCTG GACAGATGTT TTTCAAGTTG GGATCATGGT GGCTGGATTT 
GCATCCGTAA TTATACAGGC ATCAATAACT CAACATGGCA TCAATAAGAT TTTAAGTGAT 
GCTTTTAATG GTGGAAGATT AAACTTCTGG AATTTTGATC CTAACCCTTT GCAAAGACAC 
ACGTTCTGGA CAATTGTCAT TGGAGGGACT TTTACCTGGA CCACCATCTA TGGTGTCAAC 
CAGTCCCAAG TGCAGAGATA TATTTCCTGT AAAAGCAGAC TCCATGCAAA ACTGTCCCTC 
TATGTCAACC TTGTGGGCCT CTGGGTGATC CTCACCTGCT CCATATTCTG TGGGCTTGCC 
CTGTACTCCA GATACCGTGA ATGTGATCCC TGGACATCTA AGAAAGTGTC AGCGATTGAC 
CAGCTTATGC CTTATTTGGT GTTGGACATT TTGAAAAATT ACCCTGGTGT TCCCGGACTT 
TTTGTGGCCT GCGCTTACAG TGGGACATTA AGCACAGTGT CCTCCAGTAT CAATGCCTTG 
GCGGCAGTCA CCGTGGAAGA TCTCATCAAA CCCCGCTTTA AGTCTCTCTC AGAGAAGTCT 
CTGTCTTGGA TTTCCCAAGG AATGAGTGTT CTGTATGGAG CTCTGTGCAT TGGAATGGCT 
GCCCTGGCAT CGCTGATGGG AGCCTTGTTA CAGGCAGCAC TCAGCATATT CGGCATGGTT 
GGTGGACCAC TTCTGGGCTT GTTTTCTTTG GGCATCTTGG TCCCCTTCGC CAACTCGATT 
GGAGCACTCA CTGGTCTGTT GGCTGGATTT GCCATTTCCT TATGGGTAGG AATTGGAGCC 
CAGCTTTATC CTCCGCTTCC TGAGAGAACC TTACCATTGC CCCTTGAAAC CTATGGCTGT 
AACATCACGC ACAATGGGTC GGACTGGATG TCAACCACAG AAATGCCATT TTCTACTAGC 
GCTTTTCAAA TACATAATGC GGAAAGGACT CCACTGATGG ACAATTGGTA TTCATTATCA 
TATCTGTACT TCAGTACTAT TGGAACGCTG ACAACACTGT TTGTGGGGAT ACTTATCAGC 
TTATCAACAG GAGGAAGAAA ACAGAACTTA GATCCCCGAT TCTTACTAAC CAAACAGGAC 
TTTTTATCCA ATTTTGATGT TTTTAAGAAA AGGAATCATG TTTTAAACTA TAAATTGCAT 
CCTGTGGAAG TTGGTGGAAC TGATAACCCT GCCTTCAACC ATGTTGAGCT GAACTTCACA 
GATCACAGTG GCAAGATCAA TGGGACTCGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663398.2
CDS	316..2151
Translation	MDASRDIGSFVVWDYVVFAGMLLISAAIGIYYAFAGGGQQTSKDFLMGGRSMSAVPVALSLTASFMSAVTVLGTPAEVYRFGAIFSIFVITYFFVVVISAEVFLPVFYRLGITSTYEYLELRFNRCIRLCGTILFIVQTILYTGIVIYAPALALNQVTGFDLWGAVVATGVVCTFYCTLGGLKAVVWTDVFQVGIMVAGFASVIIQASITQHGINKILSDAFNGGRLNFWNFDPNPLQRHTFWTIVIGGTFTWTTIYGVNQSQVQRYISCKSRLHAKLSLYVNLVGLWVILTCSIFCGLALYSRYRECDPWTSKKVSAIDQLMPYLVLDILKNYPGVPGLFVACAYSGTLSTVSSSINALAAVTVEDLIKPRFKSLSEKSLSWISQGMSVLYGALCIGMAALASLMGALLQAALSIFGMVGGPLLGLFSLGILVPFANSIGALTGLLAGFAISLWVGIGAQLYPPLPERTLPLPLETYGCNITHNGSDWMSTTEMPFSTSAFQIHNAERTPLMDNWYSLSYLYFSTIGTLTTLFVGILISLSTGGRKQNLDPRFLLTKQDFLSNFDVFKKRNHVLNYKLHPVEVGGTDNPAFNHVELNFTDHSGKINGTRL

Target ORF information:

RefSeq Version	NM_145423.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 5 (iodide transporter), member 8 (Slc5a8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145423.2

ORF Insert Sequence:

ATGGACGCGT CGCGGGACAT CGGCAGTTTT GTGGTGTGGG ACTACGTGGT GTTCGCAGGC 
ATGCTGCTCA TCTCGGCGGC TATAGGCATC TATTACGCCT TCGCGGGGGG CGGCCAGCAG 
ACCTCCAAGG ACTTTCTTAT GGGCGGTCGC AGTATGTCCG CCGTGCCTGT GGCTCTGTCC 
CTCACCGCCA GCTTCATGTC CGCTGTCACT GTCCTGGGCA CTCCTGCCGA GGTCTACCGT 
TTTGGGGCGA TATTCAGCAT CTTTGTCATC ACCTACTTTT TTGTAGTGGT TATCAGCGCG 
GAGGTCTTCC TTCCAGTGTT CTATAGGCTG GGGATCACCA GCACCTACGA GTATTTGGAA 
CTTCGATTTA ATCGATGCAT TCGTCTCTGT GGCACAATCC TCTTCATCGT TCAAACAATT 
CTGTACACTG GAATTGTGAT TTATGCCCCT GCTCTGGCTT TGAATCAAGT TACAGGATTT 
GATCTGTGGG GCGCAGTGGT GGCCACAGGG GTGGTCTGCA CATTCTACTG CACACTGGGC 
GGTCTTAAAG CAGTTGTCTG GACAGATGTT TTTCAAGTTG GGATCATGGT GGCTGGATTT 
GCATCCGTAA TTATACAGGC ATCAATAACT CAACATGGCA TCAATAAGAT TTTAAGTGAT 
GCTTTTAATG GTGGAAGATT AAACTTCTGG AATTTTGATC CTAACCCTTT GCAAAGACAC 
ACGTTCTGGA CAATTGTCAT TGGAGGGACT TTTACCTGGA CCACCATCTA TGGTGTCAAC 
CAGTCCCAAG TGCAGAGATA TATTTCCTGT AAAAGCAGAC TCCATGCAAA ACTGTCCCTC 
TATGTCAACC TTGTGGGCCT CTGGGTGATC CTCACCTGCT CCATATTCTG TGGGCTTGCC 
CTGTACTCCA GATACCGTGA ATGTGATCCC TGGACATCTA AGAAAGTGTC AGCGATTGAC 
CAGCTTATGC CTTATTTGGT GTTGGACATT TTGAAAAATT ACCCTGGTGT TCCCGGACTT 
TTTGTGGCCT GCGCTTACAG TGGGACATTA AGCACAGTGT CCTCCAGTAT CAATGCCTTG 
GCGGCAGTCA CCGTGGAAGA TCTCATCAAA CCCCGCTTTA AGTCTCTCTC AGAGAAGTCT 
CTGTCTTGGA TTTCCCAAGG AATGAGTGTT CTGTATGGAG CTCTGTGCAT TGGAATGGCT 
GCCCTGGCAT CGCTGATGGG AGCCTTGTTA CAGGCAGCAC TCAGCATATT CGGCATGGTT 
GGTGGACCAC TTCTGGGCTT GTTTTCTTTG GGCATCTTGG TCCCCTTCGC CAACTCGATT 
GGAGCACTCA CTGGTCTGTT GGCTGGATTT GCCATTTCCT TATGGGTAGG AATTGGAGCC 
CAGCTTTATC CTCCGCTTCC TGAGAGAACC TTACCATTGC CCCTTGAAAC CTATGGCTGT 
AACATCACGC ACAATGGGTC GGACTGGATG TCAACCACAG AAATGCCATT TTCTACTAGC 
GCTTTTCAAA TACATAATGC GGAAAGGACT CCACTGATGG ACAATTGGTA TTCATTATCA 
TATCTGTACT TCAGTACTAT TGGAACGCTG ACAACACTGT TTGTGGGGAT ACTTATCAGC 
TTATCAACAG GAGGAAGAAA ACAGAACTTA GATCCCCGAT TCTTACTAAC CAAACAGGAC 
TTTTTATCCA ATTTTGATGT TTTTAAGAAA AGGAATCATG TTTTAAACTA TAAATTGCAT 
CCTGTGGAAG TTGGTGGAAC TGATAACCCT GCCTTCAACC ATGTTGAGCT GAACTTCACA 
GATCACAGTG GCAAGATCAA TGGGACTCGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SLC transporters as a novel class of tumour suppressors: identity, function and molecular mechanisms.
The Biochemical journal473(9)1113-24(2016 05)
Bhutia YD,Babu E,Ramachandran S,Yang S,Thangaraju M,Ganapathy V

Slc5a8, a Na+-coupled high-affinity transporter for short-chain fatty acids, is a conditional tumour suppressor in colon that protects against colitis and colon cancer under low-fibre dietary conditions.
The Biochemical journal469(2)267-78(2015 Jul)
Gurav A,Sivaprakasam S,Bhutia YD,Boettger T,Singh N,Ganapathy V

Molecular mechanism of SLC5A8 inactivation in breast cancer.
Molecular and cellular biology33(19)3920-35(2013 Oct)
Elangovan S,Pathania R,Ramachandran S,Ananth S,Padia RN,Srinivas SR,Babu E,Hawthorn L,Schoenlein PV,Boettger T,Smith SB,Prasad PD,Ganapathy V,Thangaraju M

Induction of the cystine/glutamate exchanger SLC7A11 in retinal pigment epithelial cells by the antipsoriatic drug monomethylfumarate.
Investigative ophthalmology & visual science54(3)1592-602(2013 Mar)
Ananth S,Babu E,Veeranan-Karmegam R,Bozard Baldowski BR,Boettger T,Martin PM

Transport via SLC5A8 (SMCT1) is obligatory for 2-oxothiazolidine-4-carboxylate to enhance glutathione production in retinal pigment epithelial cells.
Investigative ophthalmology & visual science52(8)5749-57(2011 Jul)
Babu E,Ananth S,Veeranan-Karmegam R,Coothankandaswamy V,Smith SB,Boettger T,Ganapathy V,Martin PM

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Slc5a8 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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