Nhsl1 cDNA ORF clone, Mus musculus(house mouse)

The following Nhsl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nhsl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu43848 XM_030245009.1
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu43848 XM_030245008.1
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu43848 XM_006512691.4
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu43848 XM_030245007.1
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu43848 XM_030245006.1
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu43843 XM_006512686.4
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu43844 XM_006512687.4
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu43845 XM_006512688.4
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu43846 XM_006512689.4
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu43847 XM_006512690.4
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu85408 XM_030245003.1
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu85409 XM_030245004.1
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu85410 XM_030245005.1
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu04541 NM_173390.3
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $937.30
$1339.00
OMu09232 NM_001163592.1
Latest version!
Mus musculus NHS-like 1 (Nhsl1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $930.30
$1329.00
View Old Accession Versions >>
OMu43843 XM_006512686.3 Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu43846 XM_006512689.3 Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu43845 XM_006512688.3 Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu43847 XM_006512690.3 Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu43844 XM_006512687.3 Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu43848 XM_006512691.3 Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu43848
    Clone ID Related Accession (Same CDS sequence) XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
    Accession Version XM_030245006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100866.1
    CDS873..4799
    Translation

    Target ORF information:

    RefSeq Version XM_030245006.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43848
    Clone ID Related Accession (Same CDS sequence) XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
    Accession Version XM_030245008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100868.1
    CDS805..4731
    Translation

    Target ORF information:

    RefSeq Version XM_030245008.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43848
    Clone ID Related Accession (Same CDS sequence) XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
    Accession Version XM_030245009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100869.1
    CDS917..4843
    Translation

    Target ORF information:

    RefSeq Version XM_030245009.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43848
    Clone ID Related Accession (Same CDS sequence) XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
    Accession Version XM_006512691.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512691.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512754.1
    CDS13489..17415
    Translation

    Target ORF information:

    RefSeq Version XM_006512691.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512691.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43848
    Clone ID Related Accession (Same CDS sequence) XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
    Accession Version XM_030245007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100867.1
    CDS1306..5232
    Translation

    Target ORF information:

    RefSeq Version XM_030245007.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43848
    Clone ID Related Accession (Same CDS sequence) XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
    Accession Version XM_006512691.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512691.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512754.1
    CDS1476..5402
    Translation

    Target ORF information:

    RefSeq Version XM_006512691.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512691.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
    GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC
    CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT
    TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC
    TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT
    ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT
    GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC
    TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA
    CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT
    GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA
    CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG
    GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT
    AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT
    TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT
    GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT
    AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT
    TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC
    AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG
    AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG
    AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC
    AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG
    CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC
    AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT
    AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC
    CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC
    TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG
    GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC
    CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC
    ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG
    CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA
    CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC
    TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC
    TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC
    AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG
    CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC
    ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG
    CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT
    CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA
    TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG
    CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA
    GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT
    CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG
    GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG
    AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA
    GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG
    AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA
    GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT
    ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA
    GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA
    GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA
    CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG
    AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC
    TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT
    AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT
    GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA
    CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC
    ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT
    CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC
    TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC
    CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG
    AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC
    AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA
    GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG
    GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC
    GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG
    TGTGGGGGTA CGGAACAGAA GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43843
    Clone ID Related Accession (Same CDS sequence) XM_006512686.3 , XM_006512686.4
    Accession Version XM_006512686.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512686.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
    CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC
    CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG
    GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG
    GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC
    AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG
    AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG
    CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT
    TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT
    CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA
    TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT
    CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA
    GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGGGGAGAA TTTCGATCGT
    CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACAGACACTC TGGTAAGACG ACCGAAGAAA
    GTCAAAAGGA GAAAGACAAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCATCA
    GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT
    ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC
    AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC
    AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT
    GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC
    CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT
    GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG
    GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC
    AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC
    TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT
    CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA
    AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC
    CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC
    CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA
    AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT
    GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT
    GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG
    CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG
    GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC
    TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC
    AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC
    GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC
    CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC
    GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC
    CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG
    CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC
    ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC
    AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT
    CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA
    GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT
    AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT
    TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG
    TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG
    ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA
    GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT
    CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA
    CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC
    CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT
    TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA
    CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA
    GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG
    CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT
    TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT
    TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA
    TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC
    TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT
    GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC
    CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG
    AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA
    GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT
    GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC
    CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT
    GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG
    TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC
    AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA
    TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC
    CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC
    GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC
    CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC
    ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG
    CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT
    CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT
    CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC
    CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA
    GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG
    ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT
    GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512749.1
    CDS486..5471
    Translation

    Target ORF information:

    RefSeq Version XM_006512686.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512686.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
    CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC
    CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG
    GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG
    GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC
    AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG
    AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG
    CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT
    TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT
    CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA
    TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT
    CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA
    GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGGGGAGAA TTTCGATCGT
    CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACAGACACTC TGGTAAGACG ACCGAAGAAA
    GTCAAAAGGA GAAAGACAAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCATCA
    GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT
    ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC
    AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC
    AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT
    GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC
    CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT
    GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG
    GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC
    AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC
    TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT
    CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA
    AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC
    CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC
    CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA
    AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT
    GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT
    GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG
    CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG
    GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC
    TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC
    AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC
    GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC
    CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC
    GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC
    CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG
    CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC
    ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC
    AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT
    CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA
    GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT
    AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT
    TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG
    TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG
    ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA
    GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT
    CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA
    CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC
    CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT
    TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA
    CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA
    GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG
    CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT
    TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT
    TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA
    TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC
    TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT
    GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC
    CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG
    AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA
    GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT
    GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC
    CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT
    GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG
    TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC
    AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA
    TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC
    CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC
    GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC
    CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC
    ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG
    CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT
    CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT
    CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC
    CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA
    GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG
    ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT
    GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43844
    Clone ID Related Accession (Same CDS sequence) XM_006512687.3 , XM_006512687.4
    Accession Version XM_006512687.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512687.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC
    AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA
    TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC
    CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC
    TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT
    GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT
    GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG
    TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA
    ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG
    CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT
    ATAAACATAA CTGGGGAGAA TTTCGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC
    ACAGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACAAT TTCAGGAATC
    CCTGACATCA TTCAGAAGGA GCTAGCATCA GCCACTGGCC AAGATGATGA TGGTAGTGCC
    CATTCGCTGT ACGTCCCAGA CCACTACTCT ACGTTAGGAA GGCTTGATAG TTATCGGTCC
    ACCGGGCAAT GCTTGGAAAC CAGGGACACC AGCTGTCAGA CCGAAGACGT GAAGGTCATC
    CCACCATCAA TGAGAAGGAT CAGGGCTCAC AAGGGGGTAG GCGTTGCTGC CCAGATGAGC
    CACCTCTCAG GTTCCTCAGG CAACATGTCT GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC
    CCATCTCGCC TCAGTAACGA TACTGGTTTC CACAGTCTTC CACGTACCGG CCCGAGAGCA
    AGCACCTATT CACTGGAGGG AAGGATGGGT GCCCTGGGCT CTACTGAAGA TACGGATGAC
    ACTTCTCCCT ACCAGGGGGG TAGCTTACAG GGGCATGAAA ACTTTGCGCA TTTAGGAGGT
    GCGTCGAGTA CTGGGATGCT TTCGAGGCCC AAATCCCAGC AGTTGAGGTT TTTGGAGAGT
    CCAGCATGTG TGGTTTCACC TCATGCAGCC TACTCTACCA GTGTCATCCC AAATGCCACG
    CTACTGTCCT CTTCAGAGGT TATTGTCATT CACACTGCTC AGAGTGCAGG ACAGCTGGAC
    AGTAGAACAC CCGGTTCATC TTCATATTCA AAAATAAAAC CCAGAGACCG TCCCACACCC
    AGATGTTCTG TGAAAGATGA CCATCAGTCC CCCCGTCATC ACTGGAACGA GGGTCATCTC
    ATTCATTCAC GGGCTTTAGC CTCCTCTGTC CCTGGTGCCA CCACGCTGTT ATCCCTCCAT
    GATTCAGAGG TCTCTCTAAA TGCCCCAGCA AACAGGGAAA ATGGATCCCA AGCCATACTC
    TATCATTGTA GAAACAACCC AAGCTTTCCT GACCACCCTT CAGATGTGGA TGGCAAGAGC
    GAGTGTAGTT ATTCGGGGGA CAGGGGATGT GGCAGTTCTG AGCCCTGGGA ATATAAAACC
    TCCAGCAATG GGCGGGCATC CCCACTGAAG CCACACCTGG CCACTCCTGG TTGCTCTACT
    CCCACAAGTA ACGTGAGCAG CTGCAGTTTG GACCAAACAT CTCTCAAGGG TGATACCAGG
    TCCCTGTGTT CAGAGGACCA TGATGGTTAC TATACTACCA CTCACGAGGC TGGGAACCTG
    TACACTCTCA GTGATGGCTT GGGGAACCCC AGGCACAGCA TGGTCAATGT TTTTGATGGA
    AGGGCTCAGA GAAGCCAAGG GGATCAGGCC GCTCACCAGG ATAAAATCCT TTCGAGAAAC
    ATCTCTCTGA AGAAAGCAAA GAAACCACCC CTGCCACCAT CCCGGACCGA TTCTCTGCGA
    AGGATTCCCA AAAAGAACAA CCAGACAAAC GGTCAGGTGC TCAATGAGAG CCTGATCGCC
    TCGCTCCAGC ATTCACTGCA GCTAAGCCTC CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG
    AGCCCCTGCA GTGACTTTGA GGAACCCTGG CTCCCCAGGT CCAGAAGCCA GAGCATTGTT
    AGCGAGGGTA GTAGCTTGAC CTCCACCACC ACTCCCAACG TGTATTCTCT GTGTGGGGTC
    ACTCCGTCCC AGAGCGACAC AAGTAGCGTC AAGTCGGAAT ACACGGACCC TTGGGGTTAC
    TACATTGACT ACACGAGCTT GCAGGAAGAT CCAGGGAACC CCACCGGGGG CTGCTCAGCC
    AACACTGAGG CAGCAACTGG AAACGGGCCA GTACGCCACA TCCAGGAGGG GTCGAGAGTC
    CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT AGACCAAAGA TCGCCTCACC AGAGAAGTCA
    CAGAGAGTCA CCTCTCCATC CAGTGGGTAT TCTAGCCAGT CGAATACACC CACAGCACTC
    ACCCCTGTGC CTGTGTTTTT AAAATCAATG TCACCAGCAA ATGGAAAGGG GAAGGCCAAG
    CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG ATCTCTTCTA TGTCCATCTC ATCATCGTCC
    ACTTCTCTCT CTTCTAATAC TTCTACCGAA GGAAGTGGGA CCATGAAGAA ACTGGATACA
    ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT
    CTGGCAGACA AGTCCCCTTT TCTTCCACCA CCTCCTCCTC TAGCAGATTG CTCCGAGGGC
    TCTCCTCTGC CGCCCTCTCC CATGTTCCCC CCTCCACCAC CAGAAGCCCT TGTTCCCTTC
    TGCTCCCCCA CCGACGGGTG CCTTTCTCCT TCTCCCACAG CAGTTAGCCC CTCTCTTCCA
    AGATCCTTGC CTCCTGTGCC AGCACCTCCA CCTTTCTTGC CCTCATCAGA ACCCCCGCCC
    GCTCCGCCTC TGGACCCCAA ACTCATGAAA GAAAACAGGC CCTTTTTCAA AAACTCTAGC
    CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA
    CCCCCCATGC CCCTGATCAC CACAGAAGCT TTGCAGATGG TGCAGTTGAG GCCAGTGAGG
    AAGAACTCAG GTGCCGGGGC AGTCCTGTTT TCTGAACCAT CAGCTCAGGA ACAACGAACT
    CCGACTGCTC CACAGTATCA CTTAAAGCCA TCTGCTTTCC TCAAATCCCG AAATAGCATA
    AATGAAATGG AGAGTGAAAG CCAGGCTGCC TCTGTGACGA GCTCGCTTCC GATGCCTGCC
    AAGAGCCAGA GTCAGGGTGA CCATGACAGT GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC
    AGCGATGGAG CTCCAGGCCC GGGTCCCAGC CTCAGGACCA CTCTGCTCCC AGACTCTTCA
    CCCAGCAGGA AGCCACCCCC CATCTCTAAG AAGCCCAAAC TGTTCCTGGT GGTACCACCT
    CCGCAGAGAG ATTTCACAGC GGAGCCCACA GAGAACGGGA GCGAAGCTTT CCCAGGCGTG
    CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA
    GCATCCCGAG CTGGCTCTCA TGCTACGGCC CCCACCCCCG GCAGTCCGGC TCTAGAACCG
    GGAACTGCAG GGTCCTTATC CTCTAGCATT GTGGAAGCCA ATGTCCCCAT GGTCCAACCT
    AATACATCAC CGGGCCCCAC ACAGGAAGAG TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA
    AATGCGAAGA GCTGCCTGTC TCAACAGGGC AGAGAAGCTG GCTTGCTGGA ACCCAACACA
    GCTGCTTCCT CTTCAGACCC CGTGGATGTA TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG
    ACTCCGACTA AACCCAGGAC AACAGAAGAC CTGTTTGCAG CCATTCACAG ATCTAAAAGG
    AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC GATCACACTC GAAATCACTC TCCCTCCCCG
    CCTGTGACAC CCACGAGTGC TGCCCCTAAC CTGGCCTCTC CAAAGCAAGT AGGGTCTATC
    CAGAGAAGCA TAAAAAAGAG CACTACCAGC AGTGACAACT TCAAAGCTCT GCTGCTAAAG
    AAGGGGAGTC GCTCGGACAC CAGCGCTCGC ATGTCAGCGG CTGAGATGCT CAAGTCTACG
    GACCCTCGCT TCCAGAGATC TAGGTCGGAG CCTTCGGCAG ACAGCCCGGA CAGCCCATCC
    AGCTGCTCCC CCAACAAGAA CAGGAGAGCT CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG
    ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT CGGTACAGCC GCAGCAGGAC GCCACCCTCT
    GCGGCCAGCA GCAGGTACAG CATGCGGAAC CGCATCCAGA GCAGCCCCAT GACCGTCATC
    TCCGAGGGAG AGGGAGAACC TGCTGAGCCA GCGGACAACA AAGCACGCAG GGCCCTGGAT
    GCTACCAGGG TGTGCTCTCT GGACAGACTG ACGGGTCAGG AGATGGACCA GGCCAGCCTC
    CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT GGGATAGGAA GGGCTGAGGG CAACGGTCCC
    TCGGAGCAGT GTGGGGGTAC GGAACAGAAG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512750.1
    CDS857..5752
    Translation

    Target ORF information:

    RefSeq Version XM_006512687.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512687.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC
    AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA
    TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC
    CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC
    TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT
    GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT
    GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG
    TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA
    ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG
    CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT
    ATAAACATAA CTGGGGAGAA TTTCGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC
    ACAGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACAAT TTCAGGAATC
    CCTGACATCA TTCAGAAGGA GCTAGCATCA GCCACTGGCC AAGATGATGA TGGTAGTGCC
    CATTCGCTGT ACGTCCCAGA CCACTACTCT ACGTTAGGAA GGCTTGATAG TTATCGGTCC
    ACCGGGCAAT GCTTGGAAAC CAGGGACACC AGCTGTCAGA CCGAAGACGT GAAGGTCATC
    CCACCATCAA TGAGAAGGAT CAGGGCTCAC AAGGGGGTAG GCGTTGCTGC CCAGATGAGC
    CACCTCTCAG GTTCCTCAGG CAACATGTCT GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC
    CCATCTCGCC TCAGTAACGA TACTGGTTTC CACAGTCTTC CACGTACCGG CCCGAGAGCA
    AGCACCTATT CACTGGAGGG AAGGATGGGT GCCCTGGGCT CTACTGAAGA TACGGATGAC
    ACTTCTCCCT ACCAGGGGGG TAGCTTACAG GGGCATGAAA ACTTTGCGCA TTTAGGAGGT
    GCGTCGAGTA CTGGGATGCT TTCGAGGCCC AAATCCCAGC AGTTGAGGTT TTTGGAGAGT
    CCAGCATGTG TGGTTTCACC TCATGCAGCC TACTCTACCA GTGTCATCCC AAATGCCACG
    CTACTGTCCT CTTCAGAGGT TATTGTCATT CACACTGCTC AGAGTGCAGG ACAGCTGGAC
    AGTAGAACAC CCGGTTCATC TTCATATTCA AAAATAAAAC CCAGAGACCG TCCCACACCC
    AGATGTTCTG TGAAAGATGA CCATCAGTCC CCCCGTCATC ACTGGAACGA GGGTCATCTC
    ATTCATTCAC GGGCTTTAGC CTCCTCTGTC CCTGGTGCCA CCACGCTGTT ATCCCTCCAT
    GATTCAGAGG TCTCTCTAAA TGCCCCAGCA AACAGGGAAA ATGGATCCCA AGCCATACTC
    TATCATTGTA GAAACAACCC AAGCTTTCCT GACCACCCTT CAGATGTGGA TGGCAAGAGC
    GAGTGTAGTT ATTCGGGGGA CAGGGGATGT GGCAGTTCTG AGCCCTGGGA ATATAAAACC
    TCCAGCAATG GGCGGGCATC CCCACTGAAG CCACACCTGG CCACTCCTGG TTGCTCTACT
    CCCACAAGTA ACGTGAGCAG CTGCAGTTTG GACCAAACAT CTCTCAAGGG TGATACCAGG
    TCCCTGTGTT CAGAGGACCA TGATGGTTAC TATACTACCA CTCACGAGGC TGGGAACCTG
    TACACTCTCA GTGATGGCTT GGGGAACCCC AGGCACAGCA TGGTCAATGT TTTTGATGGA
    AGGGCTCAGA GAAGCCAAGG GGATCAGGCC GCTCACCAGG ATAAAATCCT TTCGAGAAAC
    ATCTCTCTGA AGAAAGCAAA GAAACCACCC CTGCCACCAT CCCGGACCGA TTCTCTGCGA
    AGGATTCCCA AAAAGAACAA CCAGACAAAC GGTCAGGTGC TCAATGAGAG CCTGATCGCC
    TCGCTCCAGC ATTCACTGCA GCTAAGCCTC CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG
    AGCCCCTGCA GTGACTTTGA GGAACCCTGG CTCCCCAGGT CCAGAAGCCA GAGCATTGTT
    AGCGAGGGTA GTAGCTTGAC CTCCACCACC ACTCCCAACG TGTATTCTCT GTGTGGGGTC
    ACTCCGTCCC AGAGCGACAC AAGTAGCGTC AAGTCGGAAT ACACGGACCC TTGGGGTTAC
    TACATTGACT ACACGAGCTT GCAGGAAGAT CCAGGGAACC CCACCGGGGG CTGCTCAGCC
    AACACTGAGG CAGCAACTGG AAACGGGCCA GTACGCCACA TCCAGGAGGG GTCGAGAGTC
    CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT AGACCAAAGA TCGCCTCACC AGAGAAGTCA
    CAGAGAGTCA CCTCTCCATC CAGTGGGTAT TCTAGCCAGT CGAATACACC CACAGCACTC
    ACCCCTGTGC CTGTGTTTTT AAAATCAATG TCACCAGCAA ATGGAAAGGG GAAGGCCAAG
    CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG ATCTCTTCTA TGTCCATCTC ATCATCGTCC
    ACTTCTCTCT CTTCTAATAC TTCTACCGAA GGAAGTGGGA CCATGAAGAA ACTGGATACA
    ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT
    CTGGCAGACA AGTCCCCTTT TCTTCCACCA CCTCCTCCTC TAGCAGATTG CTCCGAGGGC
    TCTCCTCTGC CGCCCTCTCC CATGTTCCCC CCTCCACCAC CAGAAGCCCT TGTTCCCTTC
    TGCTCCCCCA CCGACGGGTG CCTTTCTCCT TCTCCCACAG CAGTTAGCCC CTCTCTTCCA
    AGATCCTTGC CTCCTGTGCC AGCACCTCCA CCTTTCTTGC CCTCATCAGA ACCCCCGCCC
    GCTCCGCCTC TGGACCCCAA ACTCATGAAA GAAAACAGGC CCTTTTTCAA AAACTCTAGC
    CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA
    CCCCCCATGC CCCTGATCAC CACAGAAGCT TTGCAGATGG TGCAGTTGAG GCCAGTGAGG
    AAGAACTCAG GTGCCGGGGC AGTCCTGTTT TCTGAACCAT CAGCTCAGGA ACAACGAACT
    CCGACTGCTC CACAGTATCA CTTAAAGCCA TCTGCTTTCC TCAAATCCCG AAATAGCATA
    AATGAAATGG AGAGTGAAAG CCAGGCTGCC TCTGTGACGA GCTCGCTTCC GATGCCTGCC
    AAGAGCCAGA GTCAGGGTGA CCATGACAGT GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC
    AGCGATGGAG CTCCAGGCCC GGGTCCCAGC CTCAGGACCA CTCTGCTCCC AGACTCTTCA
    CCCAGCAGGA AGCCACCCCC CATCTCTAAG AAGCCCAAAC TGTTCCTGGT GGTACCACCT
    CCGCAGAGAG ATTTCACAGC GGAGCCCACA GAGAACGGGA GCGAAGCTTT CCCAGGCGTG
    CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA
    GCATCCCGAG CTGGCTCTCA TGCTACGGCC CCCACCCCCG GCAGTCCGGC TCTAGAACCG
    GGAACTGCAG GGTCCTTATC CTCTAGCATT GTGGAAGCCA ATGTCCCCAT GGTCCAACCT
    AATACATCAC CGGGCCCCAC ACAGGAAGAG TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA
    AATGCGAAGA GCTGCCTGTC TCAACAGGGC AGAGAAGCTG GCTTGCTGGA ACCCAACACA
    GCTGCTTCCT CTTCAGACCC CGTGGATGTA TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG
    ACTCCGACTA AACCCAGGAC AACAGAAGAC CTGTTTGCAG CCATTCACAG ATCTAAAAGG
    AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC GATCACACTC GAAATCACTC TCCCTCCCCG
    CCTGTGACAC CCACGAGTGC TGCCCCTAAC CTGGCCTCTC CAAAGCAAGT AGGGTCTATC
    CAGAGAAGCA TAAAAAAGAG CACTACCAGC AGTGACAACT TCAAAGCTCT GCTGCTAAAG
    AAGGGGAGTC GCTCGGACAC CAGCGCTCGC ATGTCAGCGG CTGAGATGCT CAAGTCTACG
    GACCCTCGCT TCCAGAGATC TAGGTCGGAG CCTTCGGCAG ACAGCCCGGA CAGCCCATCC
    AGCTGCTCCC CCAACAAGAA CAGGAGAGCT CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG
    ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT CGGTACAGCC GCAGCAGGAC GCCACCCTCT
    GCGGCCAGCA GCAGGTACAG CATGCGGAAC CGCATCCAGA GCAGCCCCAT GACCGTCATC
    TCCGAGGGAG AGGGAGAACC TGCTGAGCCA GCGGACAACA AAGCACGCAG GGCCCTGGAT
    GCTACCAGGG TGTGCTCTCT GGACAGACTG ACGGGTCAGG AGATGGACCA GGCCAGCCTC
    CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT GGGATAGGAA GGGCTGAGGG CAACGGTCCC
    TCGGAGCAGT GTGGGGGTAC GGAACAGAAG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43845
    Clone ID Related Accession (Same CDS sequence) XM_006512688.3 , XM_006512688.4
    Accession Version XM_006512688.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512688.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
    CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC
    CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG
    GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG
    GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC
    AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG
    AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG
    CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT
    TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT
    CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA
    TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT
    CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA
    GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGCATCAGC CACTGGCCAA
    GATGATGATG GTAGTGCCCA TTCGCTGTAC GTCCCAGACC ACTACTCTAC GTTAGGAAGG
    CTTGATAGTT ATCGGTCCAC CGGGCAATGC TTGGAAACCA GGGACACCAG CTGTCAGACC
    GAAGACGTGA AGGTCATCCC ACCATCAATG AGAAGGATCA GGGCTCACAA GGGGGTAGGC
    GTTGCTGCCC AGATGAGCCA CCTCTCAGGT TCCTCAGGCA ACATGTCTGT GCTGAGTGAC
    TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC AGTAACGATA CTGGTTTCCA CAGTCTTCCA
    CGTACCGGCC CGAGAGCAAG CACCTATTCA CTGGAGGGAA GGATGGGTGC CCTGGGCTCT
    ACTGAAGATA CGGATGACAC TTCTCCCTAC CAGGGGGGTA GCTTACAGGG GCATGAAAAC
    TTTGCGCATT TAGGAGGTGC GTCGAGTACT GGGATGCTTT CGAGGCCCAA ATCCCAGCAG
    TTGAGGTTTT TGGAGAGTCC AGCATGTGTG GTTTCACCTC ATGCAGCCTA CTCTACCAGT
    GTCATCCCAA ATGCCACGCT ACTGTCCTCT TCAGAGGTTA TTGTCATTCA CACTGCTCAG
    AGTGCAGGAC AGCTGGACAG TAGAACACCC GGTTCATCTT CATATTCAAA AATAAAACCC
    AGAGACCGTC CCACACCCAG ATGTTCTGTG AAAGATGACC ATCAGTCCCC CCGTCATCAC
    TGGAACGAGG GTCATCTCAT TCATTCACGG GCTTTAGCCT CCTCTGTCCC TGGTGCCACC
    ACGCTGTTAT CCCTCCATGA TTCAGAGGTC TCTCTAAATG CCCCAGCAAA CAGGGAAAAT
    GGATCCCAAG CCATACTCTA TCATTGTAGA AACAACCCAA GCTTTCCTGA CCACCCTTCA
    GATGTGGATG GCAAGAGCGA GTGTAGTTAT TCGGGGGACA GGGGATGTGG CAGTTCTGAG
    CCCTGGGAAT ATAAAACCTC CAGCAATGGG CGGGCATCCC CACTGAAGCC ACACCTGGCC
    ACTCCTGGTT GCTCTACTCC CACAAGTAAC GTGAGCAGCT GCAGTTTGGA CCAAACATCT
    CTCAAGGGTG ATACCAGGTC CCTGTGTTCA GAGGACCATG ATGGTTACTA TACTACCACT
    CACGAGGCTG GGAACCTGTA CACTCTCAGT GATGGCTTGG GGAACCCCAG GCACAGCATG
    GTCAATGTTT TTGATGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCGC TCACCAGGAT
    AAAATCCTTT CGAGAAACAT CTCTCTGAAG AAAGCAAAGA AACCACCCCT GCCACCATCC
    CGGACCGATT CTCTGCGAAG GATTCCCAAA AAGAACAACC AGACAAACGG TCAGGTGCTC
    AATGAGAGCC TGATCGCCTC GCTCCAGCAT TCACTGCAGC TAAGCCTCCC TGGCAAGGGC
    GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTTTGAGG AACCCTGGCT CCCCAGGTCC
    AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG
    TATTCTCTGT GTGGGGTCAC TCCGTCCCAG AGCGACACAA GTAGCGTCAA GTCGGAATAC
    ACGGACCCTT GGGGTTACTA CATTGACTAC ACGAGCTTGC AGGAAGATCC AGGGAACCCC
    ACCGGGGGCT GCTCAGCCAA CACTGAGGCA GCAACTGGAA ACGGGCCAGT ACGCCACATC
    CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA GTGCCGGGCT GCTCAGTTAG ACCAAAGATC
    GCCTCACCAG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG
    AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAATGTC ACCAGCAAAT
    GGAAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCTATG
    TCCATCTCAT CATCGTCCAC TTCTCTCTCT TCTAATACTT CTACCGAAGG AAGTGGGACC
    ATGAAGAAAC TGGATACAAC CCTGGCCTCG GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC
    CCTCTGCCTT CTCCGTGTCT GGCAGACAAG TCCCCTTTTC TTCCACCACC TCCTCCTCTA
    GCAGATTGCT CCGAGGGCTC TCCTCTGCCG CCCTCTCCCA TGTTCCCCCC TCCACCACCA
    GAAGCCCTTG TTCCCTTCTG CTCCCCCACC GACGGGTGCC TTTCTCCTTC TCCCACAGCA
    GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT CCTGTGCCAG CACCTCCACC TTTCTTGCCC
    TCATCAGAAC CCCCGCCCGC TCCGCCTCTG GACCCCAAAC TCATGAAAGA AAACAGGCCC
    TTTTTCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC
    AAGGAGGAGG GCTGCCGACC CCCCATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG
    CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCCGGGGCAG TCCTGTTTTC TGAACCATCA
    GCTCAGGAAC AACGAACTCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC
    AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC
    TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTAGAGCGT
    GGCGGCCTTC CGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCACT
    CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCTAAGAA GCCCAAACTG
    TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC
    GAAGCTTTCC CAGGCGTGCC CAGCCCTACC AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA
    GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT GGCTCTCATG CTACGGCCCC CACCCCCGGC
    AGTCCGGCTC TAGAACCGGG AACTGCAGGG TCCTTATCCT CTAGCATTGT GGAAGCCAAT
    GTCCCCATGG TCCAACCTAA TACATCACCG GGCCCCACAC AGGAAGAGTC AGGCGAGAAC
    AGTGTGGATG GTGAGAGAAA TGCGAAGAGC TGCCTGTCTC AACAGGGCAG AGAAGCTGGC
    TTGCTGGAAC CCAACACAGC TGCTTCCTCT TCAGACCCCG TGGATGTATC TAAGGAAGAG
    GGAAGCGATG AGGTGCTGAC TCCGACTAAA CCCAGGACAA CAGAAGACCT GTTTGCAGCC
    ATTCACAGAT CTAAAAGGAA AGTCCTTGGC CGGAAAGACT CAGAAGACGA TCACACTCGA
    AATCACTCTC CCTCCCCGCC TGTGACACCC ACGAGTGCTG CCCCTAACCT GGCCTCTCCA
    AAGCAAGTAG GGTCTATCCA GAGAAGCATA AAAAAGAGCA CTACCAGCAG TGACAACTTC
    AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCAGCGGCT
    GAGATGCTCA AGTCTACGGA CCCTCGCTTC CAGAGATCTA GGTCGGAGCC TTCGGCAGAC
    AGCCCGGACA GCCCATCCAG CTGCTCCCCC AACAAGAACA GGAGAGCTCA GGAAGAGTGG
    GCCAAGAACG AAGGCCTGAT GCCTCGGAGT CTGTCCTTTT CGGGCCCTCG GTACAGCCGC
    AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGCGGAACCG CATCCAGAGC
    AGCCCCATGA CCGTCATCTC CGAGGGAGAG GGAGAACCTG CTGAGCCAGC GGACAACAAA
    GCACGCAGGG CCCTGGATGC TACCAGGGTG TGCTCTCTGG ACAGACTGAC GGGTCAGGAG
    ATGGACCAGG CCAGCCTCCT CTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG
    GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT GGGGGTACGG AACAGAAGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512751.1
    CDS476..5329
    Translation

    Target ORF information:

    RefSeq Version XM_006512688.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512688.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
    CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC
    CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG
    GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG
    GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC
    AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG
    AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG
    CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT
    TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT
    CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA
    TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT
    CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA
    GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGCATCAGC CACTGGCCAA
    GATGATGATG GTAGTGCCCA TTCGCTGTAC GTCCCAGACC ACTACTCTAC GTTAGGAAGG
    CTTGATAGTT ATCGGTCCAC CGGGCAATGC TTGGAAACCA GGGACACCAG CTGTCAGACC
    GAAGACGTGA AGGTCATCCC ACCATCAATG AGAAGGATCA GGGCTCACAA GGGGGTAGGC
    GTTGCTGCCC AGATGAGCCA CCTCTCAGGT TCCTCAGGCA ACATGTCTGT GCTGAGTGAC
    TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC AGTAACGATA CTGGTTTCCA CAGTCTTCCA
    CGTACCGGCC CGAGAGCAAG CACCTATTCA CTGGAGGGAA GGATGGGTGC CCTGGGCTCT
    ACTGAAGATA CGGATGACAC TTCTCCCTAC CAGGGGGGTA GCTTACAGGG GCATGAAAAC
    TTTGCGCATT TAGGAGGTGC GTCGAGTACT GGGATGCTTT CGAGGCCCAA ATCCCAGCAG
    TTGAGGTTTT TGGAGAGTCC AGCATGTGTG GTTTCACCTC ATGCAGCCTA CTCTACCAGT
    GTCATCCCAA ATGCCACGCT ACTGTCCTCT TCAGAGGTTA TTGTCATTCA CACTGCTCAG
    AGTGCAGGAC AGCTGGACAG TAGAACACCC GGTTCATCTT CATATTCAAA AATAAAACCC
    AGAGACCGTC CCACACCCAG ATGTTCTGTG AAAGATGACC ATCAGTCCCC CCGTCATCAC
    TGGAACGAGG GTCATCTCAT TCATTCACGG GCTTTAGCCT CCTCTGTCCC TGGTGCCACC
    ACGCTGTTAT CCCTCCATGA TTCAGAGGTC TCTCTAAATG CCCCAGCAAA CAGGGAAAAT
    GGATCCCAAG CCATACTCTA TCATTGTAGA AACAACCCAA GCTTTCCTGA CCACCCTTCA
    GATGTGGATG GCAAGAGCGA GTGTAGTTAT TCGGGGGACA GGGGATGTGG CAGTTCTGAG
    CCCTGGGAAT ATAAAACCTC CAGCAATGGG CGGGCATCCC CACTGAAGCC ACACCTGGCC
    ACTCCTGGTT GCTCTACTCC CACAAGTAAC GTGAGCAGCT GCAGTTTGGA CCAAACATCT
    CTCAAGGGTG ATACCAGGTC CCTGTGTTCA GAGGACCATG ATGGTTACTA TACTACCACT
    CACGAGGCTG GGAACCTGTA CACTCTCAGT GATGGCTTGG GGAACCCCAG GCACAGCATG
    GTCAATGTTT TTGATGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCGC TCACCAGGAT
    AAAATCCTTT CGAGAAACAT CTCTCTGAAG AAAGCAAAGA AACCACCCCT GCCACCATCC
    CGGACCGATT CTCTGCGAAG GATTCCCAAA AAGAACAACC AGACAAACGG TCAGGTGCTC
    AATGAGAGCC TGATCGCCTC GCTCCAGCAT TCACTGCAGC TAAGCCTCCC TGGCAAGGGC
    GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTTTGAGG AACCCTGGCT CCCCAGGTCC
    AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG
    TATTCTCTGT GTGGGGTCAC TCCGTCCCAG AGCGACACAA GTAGCGTCAA GTCGGAATAC
    ACGGACCCTT GGGGTTACTA CATTGACTAC ACGAGCTTGC AGGAAGATCC AGGGAACCCC
    ACCGGGGGCT GCTCAGCCAA CACTGAGGCA GCAACTGGAA ACGGGCCAGT ACGCCACATC
    CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA GTGCCGGGCT GCTCAGTTAG ACCAAAGATC
    GCCTCACCAG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG
    AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAATGTC ACCAGCAAAT
    GGAAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCTATG
    TCCATCTCAT CATCGTCCAC TTCTCTCTCT TCTAATACTT CTACCGAAGG AAGTGGGACC
    ATGAAGAAAC TGGATACAAC CCTGGCCTCG GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC
    CCTCTGCCTT CTCCGTGTCT GGCAGACAAG TCCCCTTTTC TTCCACCACC TCCTCCTCTA
    GCAGATTGCT CCGAGGGCTC TCCTCTGCCG CCCTCTCCCA TGTTCCCCCC TCCACCACCA
    GAAGCCCTTG TTCCCTTCTG CTCCCCCACC GACGGGTGCC TTTCTCCTTC TCCCACAGCA
    GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT CCTGTGCCAG CACCTCCACC TTTCTTGCCC
    TCATCAGAAC CCCCGCCCGC TCCGCCTCTG GACCCCAAAC TCATGAAAGA AAACAGGCCC
    TTTTTCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC
    AAGGAGGAGG GCTGCCGACC CCCCATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG
    CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCCGGGGCAG TCCTGTTTTC TGAACCATCA
    GCTCAGGAAC AACGAACTCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC
    AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC
    TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTAGAGCGT
    GGCGGCCTTC CGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCACT
    CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCTAAGAA GCCCAAACTG
    TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC
    GAAGCTTTCC CAGGCGTGCC CAGCCCTACC AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA
    GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT GGCTCTCATG CTACGGCCCC CACCCCCGGC
    AGTCCGGCTC TAGAACCGGG AACTGCAGGG TCCTTATCCT CTAGCATTGT GGAAGCCAAT
    GTCCCCATGG TCCAACCTAA TACATCACCG GGCCCCACAC AGGAAGAGTC AGGCGAGAAC
    AGTGTGGATG GTGAGAGAAA TGCGAAGAGC TGCCTGTCTC AACAGGGCAG AGAAGCTGGC
    TTGCTGGAAC CCAACACAGC TGCTTCCTCT TCAGACCCCG TGGATGTATC TAAGGAAGAG
    GGAAGCGATG AGGTGCTGAC TCCGACTAAA CCCAGGACAA CAGAAGACCT GTTTGCAGCC
    ATTCACAGAT CTAAAAGGAA AGTCCTTGGC CGGAAAGACT CAGAAGACGA TCACACTCGA
    AATCACTCTC CCTCCCCGCC TGTGACACCC ACGAGTGCTG CCCCTAACCT GGCCTCTCCA
    AAGCAAGTAG GGTCTATCCA GAGAAGCATA AAAAAGAGCA CTACCAGCAG TGACAACTTC
    AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCAGCGGCT
    GAGATGCTCA AGTCTACGGA CCCTCGCTTC CAGAGATCTA GGTCGGAGCC TTCGGCAGAC
    AGCCCGGACA GCCCATCCAG CTGCTCCCCC AACAAGAACA GGAGAGCTCA GGAAGAGTGG
    GCCAAGAACG AAGGCCTGAT GCCTCGGAGT CTGTCCTTTT CGGGCCCTCG GTACAGCCGC
    AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGCGGAACCG CATCCAGAGC
    AGCCCCATGA CCGTCATCTC CGAGGGAGAG GGAGAACCTG CTGAGCCAGC GGACAACAAA
    GCACGCAGGG CCCTGGATGC TACCAGGGTG TGCTCTCTGG ACAGACTGAC GGGTCAGGAG
    ATGGACCAGG CCAGCCTCCT CTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG
    GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT GGGGGTACGG AACAGAAGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43846
    Clone ID Related Accession (Same CDS sequence) XM_006512689.3 , XM_006512689.4
    Accession Version XM_006512689.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512689.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
    CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG
    CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC
    CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT
    GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC
    TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT
    GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC
    GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG
    ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGGGGAG
    AATTTCGATC GTCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA
    CGACCGAAGA AAGTCAAAAG GAGAAAGACA ATTTCAGGAA TCCCTGACAT CATTCAGAAG
    GAGCTAGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA
    GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA
    ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG
    ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA
    GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC
    GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG
    GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG
    GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG
    CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA
    CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG
    GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA
    TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT
    GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA
    GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA
    AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC
    CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG
    GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA
    TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC
    AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC
    CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC
    TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA
    GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA
    AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC
    AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG
    CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT
    GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG
    ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC
    ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC
    TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT
    GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG
    GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA
    TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT
    TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA
    AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT
    ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT
    GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT
    TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT
    CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG
    TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG
    CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC
    AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG
    GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC
    ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG
    GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT
    CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT
    GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC
    CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC
    CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA
    GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA
    GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT
    CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA
    TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC
    ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG
    TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC
    CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG
    ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA
    GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT
    GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG
    AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC
    ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA
    TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG
    AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA
    CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT
    CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT
    GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT
    ACGGAACAGA AGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512752.1
    CDS324..5081
    Translation

    Target ORF information:

    RefSeq Version XM_006512689.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512689.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
    CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG
    CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC
    CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT
    GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC
    TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT
    GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC
    GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG
    ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGGGGAG
    AATTTCGATC GTCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA
    CGACCGAAGA AAGTCAAAAG GAGAAAGACA ATTTCAGGAA TCCCTGACAT CATTCAGAAG
    GAGCTAGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA
    GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA
    ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG
    ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA
    GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC
    GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG
    GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG
    GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG
    CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA
    CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG
    GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA
    TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT
    GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA
    GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA
    AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC
    CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG
    GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA
    TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC
    AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC
    CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC
    TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA
    GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA
    AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC
    AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG
    CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT
    GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG
    ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC
    ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC
    TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT
    GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG
    GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA
    TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT
    TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA
    AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT
    ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT
    GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT
    TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT
    CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG
    TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG
    CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC
    AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG
    GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC
    ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG
    GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT
    CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT
    GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC
    CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC
    CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA
    GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA
    GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT
    CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA
    TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC
    ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG
    TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC
    CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG
    ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA
    GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT
    GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG
    AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC
    ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA
    TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG
    AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA
    CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT
    CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT
    GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT
    ACGGAACAGA AGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43847
    Clone ID Related Accession (Same CDS sequence) XM_006512690.4 , XM_006512690.3
    Accession Version XM_006512690.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512690.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
    ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG
    CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC
    GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT
    CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA
    GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA
    CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC
    ATAACTGGGG AGAATTTCGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC
    ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CAATTTCAGG AATCCCTGAC
    ATCATTCAGA AGGAGCTAGC ATCAGCCACT GGCCAAGATG ATGATGGTAG TGCCCATTCG
    CTGTACGTCC CAGACCACTA CTCTACGTTA GGAAGGCTTG ATAGTTATCG GTCCACCGGG
    CAATGCTTGG AAACCAGGGA CACCAGCTGT CAGACCGAAG ACGTGAAGGT CATCCCACCA
    TCAATGAGAA GGATCAGGGC TCACAAGGGG GTAGGCGTTG CTGCCCAGAT GAGCCACCTC
    TCAGGTTCCT CAGGCAACAT GTCTGTGCTG AGTGACTCAG CCGGTGTTGT GTTCCCATCT
    CGCCTCAGTA ACGATACTGG TTTCCACAGT CTTCCACGTA CCGGCCCGAG AGCAAGCACC
    TATTCACTGG AGGGAAGGAT GGGTGCCCTG GGCTCTACTG AAGATACGGA TGACACTTCT
    CCCTACCAGG GGGGTAGCTT ACAGGGGCAT GAAAACTTTG CGCATTTAGG AGGTGCGTCG
    AGTACTGGGA TGCTTTCGAG GCCCAAATCC CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA
    TGTGTGGTTT CACCTCATGC AGCCTACTCT ACCAGTGTCA TCCCAAATGC CACGCTACTG
    TCCTCTTCAG AGGTTATTGT CATTCACACT GCTCAGAGTG CAGGACAGCT GGACAGTAGA
    ACACCCGGTT CATCTTCATA TTCAAAAATA AAACCCAGAG ACCGTCCCAC ACCCAGATGT
    TCTGTGAAAG ATGACCATCA GTCCCCCCGT CATCACTGGA ACGAGGGTCA TCTCATTCAT
    TCACGGGCTT TAGCCTCCTC TGTCCCTGGT GCCACCACGC TGTTATCCCT CCATGATTCA
    GAGGTCTCTC TAAATGCCCC AGCAAACAGG GAAAATGGAT CCCAAGCCAT ACTCTATCAT
    TGTAGAAACA ACCCAAGCTT TCCTGACCAC CCTTCAGATG TGGATGGCAA GAGCGAGTGT
    AGTTATTCGG GGGACAGGGG ATGTGGCAGT TCTGAGCCCT GGGAATATAA AACCTCCAGC
    AATGGGCGGG CATCCCCACT GAAGCCACAC CTGGCCACTC CTGGTTGCTC TACTCCCACA
    AGTAACGTGA GCAGCTGCAG TTTGGACCAA ACATCTCTCA AGGGTGATAC CAGGTCCCTG
    TGTTCAGAGG ACCATGATGG TTACTATACT ACCACTCACG AGGCTGGGAA CCTGTACACT
    CTCAGTGATG GCTTGGGGAA CCCCAGGCAC AGCATGGTCA ATGTTTTTGA TGGAAGGGCT
    CAGAGAAGCC AAGGGGATCA GGCCGCTCAC CAGGATAAAA TCCTTTCGAG AAACATCTCT
    CTGAAGAAAG CAAAGAAACC ACCCCTGCCA CCATCCCGGA CCGATTCTCT GCGAAGGATT
    CCCAAAAAGA ACAACCAGAC AAACGGTCAG GTGCTCAATG AGAGCCTGAT CGCCTCGCTC
    CAGCATTCAC TGCAGCTAAG CCTCCCTGGC AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC
    TGCAGTGACT TTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG
    GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGTGG GGTCACTCCG
    TCCCAGAGCG ACACAAGTAG CGTCAAGTCG GAATACACGG ACCCTTGGGG TTACTACATT
    GACTACACGA GCTTGCAGGA AGATCCAGGG AACCCCACCG GGGGCTGCTC AGCCAACACT
    GAGGCAGCAA CTGGAAACGG GCCAGTACGC CACATCCAGG AGGGGTCGAG AGTCCCAGTG
    CCCCAAGTGC CGGGCTGCTC AGTTAGACCA AAGATCGCCT CACCAGAGAA GTCACAGAGA
    GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCT
    GTGCCTGTGT TTTTAAAATC AATGTCACCA GCAAATGGAA AGGGGAAGGC CAAGCCCAAG
    GTACCAGAAA GAAAATCGTC TCTGATCTCT TCTATGTCCA TCTCATCATC GTCCACTTCT
    CTCTCTTCTA ATACTTCTAC CGAAGGAAGT GGGACCATGA AGAAACTGGA TACAACCCTG
    GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA
    GACAAGTCCC CTTTTCTTCC ACCACCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT
    CTGCCGCCCT CTCCCATGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC
    CCCACCGACG GGTGCCTTTC TCCTTCTCCC ACAGCAGTTA GCCCCTCTCT TCCAAGATCC
    TTGCCTCCTG TGCCAGCACC TCCACCTTTC TTGCCCTCAT CAGAACCCCC GCCCGCTCCG
    CCTCTGGACC CCAAACTCAT GAAAGAAAAC AGGCCCTTTT TCAAAAACTC TAGCCAGTCC
    GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCCCC
    ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC
    TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA CCATCAGCTC AGGAACAACG AACTCCGACT
    GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA
    ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC
    CAGAGTCAGG GTGACCATGA CAGTGCAGTA GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT
    GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCACTCTGC TCCCAGACTC TTCACCCAGC
    AGGAAGCCAC CCCCCATCTC TAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG
    AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC
    CCTACCAGGG CAGAGGGGGA AGCTGTGCGG AGCCAAGAGG AGAAATCCAG CCCAGCATCC
    CGAGCTGGCT CTCATGCTAC GGCCCCCACC CCCGGCAGTC CGGCTCTAGA ACCGGGAACT
    GCAGGGTCCT TATCCTCTAG CATTGTGGAA GCCAATGTCC CCATGGTCCA ACCTAATACA
    TCACCGGGCC CCACACAGGA AGAGTCAGGC GAGAACAGTG TGGATGGTGA GAGAAATGCG
    AAGAGCTGCC TGTCTCAACA GGGCAGAGAA GCTGGCTTGC TGGAACCCAA CACAGCTGCT
    TCCTCTTCAG ACCCCGTGGA TGTATCTAAG GAAGAGGGAA GCGATGAGGT GCTGACTCCG
    ACTAAACCCA GGACAACAGA AGACCTGTTT GCAGCCATTC ACAGATCTAA AAGGAAAGTC
    CTTGGCCGGA AAGACTCAGA AGACGATCAC ACTCGAAATC ACTCTCCCTC CCCGCCTGTG
    ACACCCACGA GTGCTGCCCC TAACCTGGCC TCTCCAAAGC AAGTAGGGTC TATCCAGAGA
    AGCATAAAAA AGAGCACTAC CAGCAGTGAC AACTTCAAAG CTCTGCTGCT AAAGAAGGGG
    AGTCGCTCGG ACACCAGCGC TCGCATGTCA GCGGCTGAGA TGCTCAAGTC TACGGACCCT
    CGCTTCCAGA GATCTAGGTC GGAGCCTTCG GCAGACAGCC CGGACAGCCC ATCCAGCTGC
    TCCCCCAACA AGAACAGGAG AGCTCAGGAA GAGTGGGCCA AGAACGAAGG CCTGATGCCT
    CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC
    AGCAGCAGGT ACAGCATGCG GAACCGCATC CAGAGCAGCC CCATGACCGT CATCTCCGAG
    GGAGAGGGAG AACCTGCTGA GCCAGCGGAC AACAAAGCAC GCAGGGCCCT GGATGCTACC
    AGGGTGTGCT CTCTGGACAG ACTGACGGGT CAGGAGATGG ACCAGGCCAG CCTCCTCTGT
    AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTG AGGGCAACGG TCCCTCGGAG
    CAGTGTGGGG GTACGGAACA GAAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512753.1
    CDS5896..10605
    Translation

    Target ORF information:

    RefSeq Version XM_006512690.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512690.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
    ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG
    CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC
    GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT
    CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA
    GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA
    CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC
    ATAACTGGGG AGAATTTCGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC
    ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CAATTTCAGG AATCCCTGAC
    ATCATTCAGA AGGAGCTAGC ATCAGCCACT GGCCAAGATG ATGATGGTAG TGCCCATTCG
    CTGTACGTCC CAGACCACTA CTCTACGTTA GGAAGGCTTG ATAGTTATCG GTCCACCGGG
    CAATGCTTGG AAACCAGGGA CACCAGCTGT CAGACCGAAG ACGTGAAGGT CATCCCACCA
    TCAATGAGAA GGATCAGGGC TCACAAGGGG GTAGGCGTTG CTGCCCAGAT GAGCCACCTC
    TCAGGTTCCT CAGGCAACAT GTCTGTGCTG AGTGACTCAG CCGGTGTTGT GTTCCCATCT
    CGCCTCAGTA ACGATACTGG TTTCCACAGT CTTCCACGTA CCGGCCCGAG AGCAAGCACC
    TATTCACTGG AGGGAAGGAT GGGTGCCCTG GGCTCTACTG AAGATACGGA TGACACTTCT
    CCCTACCAGG GGGGTAGCTT ACAGGGGCAT GAAAACTTTG CGCATTTAGG AGGTGCGTCG
    AGTACTGGGA TGCTTTCGAG GCCCAAATCC CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA
    TGTGTGGTTT CACCTCATGC AGCCTACTCT ACCAGTGTCA TCCCAAATGC CACGCTACTG
    TCCTCTTCAG AGGTTATTGT CATTCACACT GCTCAGAGTG CAGGACAGCT GGACAGTAGA
    ACACCCGGTT CATCTTCATA TTCAAAAATA AAACCCAGAG ACCGTCCCAC ACCCAGATGT
    TCTGTGAAAG ATGACCATCA GTCCCCCCGT CATCACTGGA ACGAGGGTCA TCTCATTCAT
    TCACGGGCTT TAGCCTCCTC TGTCCCTGGT GCCACCACGC TGTTATCCCT CCATGATTCA
    GAGGTCTCTC TAAATGCCCC AGCAAACAGG GAAAATGGAT CCCAAGCCAT ACTCTATCAT
    TGTAGAAACA ACCCAAGCTT TCCTGACCAC CCTTCAGATG TGGATGGCAA GAGCGAGTGT
    AGTTATTCGG GGGACAGGGG ATGTGGCAGT TCTGAGCCCT GGGAATATAA AACCTCCAGC
    AATGGGCGGG CATCCCCACT GAAGCCACAC CTGGCCACTC CTGGTTGCTC TACTCCCACA
    AGTAACGTGA GCAGCTGCAG TTTGGACCAA ACATCTCTCA AGGGTGATAC CAGGTCCCTG
    TGTTCAGAGG ACCATGATGG TTACTATACT ACCACTCACG AGGCTGGGAA CCTGTACACT
    CTCAGTGATG GCTTGGGGAA CCCCAGGCAC AGCATGGTCA ATGTTTTTGA TGGAAGGGCT
    CAGAGAAGCC AAGGGGATCA GGCCGCTCAC CAGGATAAAA TCCTTTCGAG AAACATCTCT
    CTGAAGAAAG CAAAGAAACC ACCCCTGCCA CCATCCCGGA CCGATTCTCT GCGAAGGATT
    CCCAAAAAGA ACAACCAGAC AAACGGTCAG GTGCTCAATG AGAGCCTGAT CGCCTCGCTC
    CAGCATTCAC TGCAGCTAAG CCTCCCTGGC AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC
    TGCAGTGACT TTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG
    GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGTGG GGTCACTCCG
    TCCCAGAGCG ACACAAGTAG CGTCAAGTCG GAATACACGG ACCCTTGGGG TTACTACATT
    GACTACACGA GCTTGCAGGA AGATCCAGGG AACCCCACCG GGGGCTGCTC AGCCAACACT
    GAGGCAGCAA CTGGAAACGG GCCAGTACGC CACATCCAGG AGGGGTCGAG AGTCCCAGTG
    CCCCAAGTGC CGGGCTGCTC AGTTAGACCA AAGATCGCCT CACCAGAGAA GTCACAGAGA
    GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCT
    GTGCCTGTGT TTTTAAAATC AATGTCACCA GCAAATGGAA AGGGGAAGGC CAAGCCCAAG
    GTACCAGAAA GAAAATCGTC TCTGATCTCT TCTATGTCCA TCTCATCATC GTCCACTTCT
    CTCTCTTCTA ATACTTCTAC CGAAGGAAGT GGGACCATGA AGAAACTGGA TACAACCCTG
    GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA
    GACAAGTCCC CTTTTCTTCC ACCACCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT
    CTGCCGCCCT CTCCCATGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC
    CCCACCGACG GGTGCCTTTC TCCTTCTCCC ACAGCAGTTA GCCCCTCTCT TCCAAGATCC
    TTGCCTCCTG TGCCAGCACC TCCACCTTTC TTGCCCTCAT CAGAACCCCC GCCCGCTCCG
    CCTCTGGACC CCAAACTCAT GAAAGAAAAC AGGCCCTTTT TCAAAAACTC TAGCCAGTCC
    GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCCCC
    ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC
    TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA CCATCAGCTC AGGAACAACG AACTCCGACT
    GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA
    ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC
    CAGAGTCAGG GTGACCATGA CAGTGCAGTA GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT
    GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCACTCTGC TCCCAGACTC TTCACCCAGC
    AGGAAGCCAC CCCCCATCTC TAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG
    AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC
    CCTACCAGGG CAGAGGGGGA AGCTGTGCGG AGCCAAGAGG AGAAATCCAG CCCAGCATCC
    CGAGCTGGCT CTCATGCTAC GGCCCCCACC CCCGGCAGTC CGGCTCTAGA ACCGGGAACT
    GCAGGGTCCT TATCCTCTAG CATTGTGGAA GCCAATGTCC CCATGGTCCA ACCTAATACA
    TCACCGGGCC CCACACAGGA AGAGTCAGGC GAGAACAGTG TGGATGGTGA GAGAAATGCG
    AAGAGCTGCC TGTCTCAACA GGGCAGAGAA GCTGGCTTGC TGGAACCCAA CACAGCTGCT
    TCCTCTTCAG ACCCCGTGGA TGTATCTAAG GAAGAGGGAA GCGATGAGGT GCTGACTCCG
    ACTAAACCCA GGACAACAGA AGACCTGTTT GCAGCCATTC ACAGATCTAA AAGGAAAGTC
    CTTGGCCGGA AAGACTCAGA AGACGATCAC ACTCGAAATC ACTCTCCCTC CCCGCCTGTG
    ACACCCACGA GTGCTGCCCC TAACCTGGCC TCTCCAAAGC AAGTAGGGTC TATCCAGAGA
    AGCATAAAAA AGAGCACTAC CAGCAGTGAC AACTTCAAAG CTCTGCTGCT AAAGAAGGGG
    AGTCGCTCGG ACACCAGCGC TCGCATGTCA GCGGCTGAGA TGCTCAAGTC TACGGACCCT
    CGCTTCCAGA GATCTAGGTC GGAGCCTTCG GCAGACAGCC CGGACAGCCC ATCCAGCTGC
    TCCCCCAACA AGAACAGGAG AGCTCAGGAA GAGTGGGCCA AGAACGAAGG CCTGATGCCT
    CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC
    AGCAGCAGGT ACAGCATGCG GAACCGCATC CAGAGCAGCC CCATGACCGT CATCTCCGAG
    GGAGAGGGAG AACCTGCTGA GCCAGCGGAC AACAAAGCAC GCAGGGCCCT GGATGCTACC
    AGGGTGTGCT CTCTGGACAG ACTGACGGGT CAGGAGATGG ACCAGGCCAG CCTCCTCTGT
    AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTG AGGGCAACGG TCCCTCGGAG
    CAGTGTGGGG GTACGGAACA GAAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85408
    Clone ID Related Accession (Same CDS sequence) XM_030245003.1
    Accession Version XM_030245003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
    CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG
    CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC
    CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT
    GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC
    TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT
    GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC
    GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG
    ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGCATCA
    GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT
    ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC
    AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC
    AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT
    GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC
    CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT
    GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG
    GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC
    AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC
    TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT
    CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA
    AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC
    CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC
    CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA
    AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT
    GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT
    GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG
    CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG
    GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC
    TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC
    AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC
    GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC
    CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC
    GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC
    CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG
    CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC
    ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC
    AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT
    CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA
    GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT
    AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT
    TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG
    TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG
    ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA
    GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT
    CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA
    CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC
    CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT
    TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA
    CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA
    GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG
    CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT
    TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT
    TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA
    TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC
    TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT
    GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC
    CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG
    AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA
    GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT
    GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC
    CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT
    GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG
    TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC
    AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA
    TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC
    CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC
    GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC
    CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC
    ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG
    CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT
    CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT
    CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC
    CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA
    GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG
    ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT
    GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100863.1
    CDS321..4946
    Translation

    Target ORF information:

    RefSeq Version XM_030245003.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
    CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG
    CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC
    CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT
    GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC
    TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT
    GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC
    GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG
    ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGCATCA
    GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT
    ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC
    AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC
    AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT
    GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC
    CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT
    GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG
    GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC
    AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC
    TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT
    CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA
    AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC
    CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC
    CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA
    AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT
    GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT
    GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG
    CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG
    GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC
    TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC
    AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC
    GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC
    CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC
    GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC
    CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG
    CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC
    ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC
    AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT
    CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA
    GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT
    AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT
    TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG
    TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG
    ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA
    GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT
    CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA
    CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC
    CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT
    TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA
    CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA
    GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG
    CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT
    TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT
    TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA
    TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC
    TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT
    GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC
    CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG
    AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA
    GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT
    GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC
    CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT
    GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG
    TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC
    AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA
    TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC
    CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC
    GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC
    CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC
    ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG
    CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT
    CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT
    CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC
    CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA
    GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG
    ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT
    GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85409
    Clone ID Related Accession (Same CDS sequence) XM_030245004.1
    Accession Version XM_030245004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGTGGTCT TCATTAATGC AAAGATTAAA TCTTTCTTTA AACTTTTTAA AAAGAAAACG 
    GTTTCCAACT TGGACGAGGA GAGTCGATGG ACTGTCCACT ACACGGCACC ATGGCATCAG
    CAAGAGAATG TGTTCCTTCC CGCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCA AGATGGCTGC
    CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCGGCTCCAG CCCCTGCGAC
    GATTATCAAG ATGAAGACAC AGAAGCAGAC CGAAAGTGCT CTCTCTCTTC ATCTGAAGAA
    GAAAGATTTA TCGGAATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCCGACCTT
    CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACGCGG
    CAGCAAGCCC AGACGGTCCA GGCTGACGTG GTTCCTATAA ACATAACTGC ATCAGCCACT
    GGCCAAGATG ATGATGGTAG TGCCCATTCG CTGTACGTCC CAGACCACTA CTCTACGTTA
    GGAAGGCTTG ATAGTTATCG GTCCACCGGG CAATGCTTGG AAACCAGGGA CACCAGCTGT
    CAGACCGAAG ACGTGAAGGT CATCCCACCA TCAATGAGAA GGATCAGGGC TCACAAGGGG
    GTAGGCGTTG CTGCCCAGAT GAGCCACCTC TCAGGTTCCT CAGGCAACAT GTCTGTGCTG
    AGTGACTCAG CCGGTGTTGT GTTCCCATCT CGCCTCAGTA ACGATACTGG TTTCCACAGT
    CTTCCACGTA CCGGCCCGAG AGCAAGCACC TATTCACTGG AGGGAAGGAT GGGTGCCCTG
    GGCTCTACTG AAGATACGGA TGACACTTCT CCCTACCAGG GGGGTAGCTT ACAGGGGCAT
    GAAAACTTTG CGCATTTAGG AGGTGCGTCG AGTACTGGGA TGCTTTCGAG GCCCAAATCC
    CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA TGTGTGGTTT CACCTCATGC AGCCTACTCT
    ACCAGTGTCA TCCCAAATGC CACGCTACTG TCCTCTTCAG AGGTTATTGT CATTCACACT
    GCTCAGAGTG CAGGACAGCT GGACAGTAGA ACACCCGGTT CATCTTCATA TTCAAAAATA
    AAACCCAGAG ACCGTCCCAC ACCCAGATGT TCTGTGAAAG ATGACCATCA GTCCCCCCGT
    CATCACTGGA ACGAGGGTCA TCTCATTCAT TCACGGGCTT TAGCCTCCTC TGTCCCTGGT
    GCCACCACGC TGTTATCCCT CCATGATTCA GAGGTCTCTC TAAATGCCCC AGCAAACAGG
    GAAAATGGAT CCCAAGCCAT ACTCTATCAT TGTAGAAACA ACCCAAGCTT TCCTGACCAC
    CCTTCAGATG TGGATGGCAA GAGCGAGTGT AGTTATTCGG GGGACAGGGG ATGTGGCAGT
    TCTGAGCCCT GGGAATATAA AACCTCCAGC AATGGGCGGG CATCCCCACT GAAGCCACAC
    CTGGCCACTC CTGGTTGCTC TACTCCCACA AGTAACGTGA GCAGCTGCAG TTTGGACCAA
    ACATCTCTCA AGGGTGATAC CAGGTCCCTG TGTTCAGAGG ACCATGATGG TTACTATACT
    ACCACTCACG AGGCTGGGAA CCTGTACACT CTCAGTGATG GCTTGGGGAA CCCCAGGCAC
    AGCATGGTCA ATGTTTTTGA TGGAAGGGCT CAGAGAAGCC AAGGGGATCA GGCCGCTCAC
    CAGGATAAAA TCCTTTCGAG AAACATCTCT CTGAAGAAAG CAAAGAAACC ACCCCTGCCA
    CCATCCCGGA CCGATTCTCT GCGAAGGATT CCCAAAAAGA ACAACCAGAC AAACGGTCAG
    GTGCTCAATG AGAGCCTGAT CGCCTCGCTC CAGCATTCAC TGCAGCTAAG CCTCCCTGGC
    AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC TGCAGTGACT TTGAGGAACC CTGGCTCCCC
    AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG GGTAGTAGCT TGACCTCCAC CACCACTCCC
    AACGTGTATT CTCTGTGTGG GGTCACTCCG TCCCAGAGCG ACACAAGTAG CGTCAAGTCG
    GAATACACGG ACCCTTGGGG TTACTACATT GACTACACGA GCTTGCAGGA AGATCCAGGG
    AACCCCACCG GGGGCTGCTC AGCCAACACT GAGGCAGCAA CTGGAAACGG GCCAGTACGC
    CACATCCAGG AGGGGTCGAG AGTCCCAGTG CCCCAAGTGC CGGGCTGCTC AGTTAGACCA
    AAGATCGCCT CACCAGAGAA GTCACAGAGA GTCACCTCTC CATCCAGTGG GTATTCTAGC
    CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AATGTCACCA
    GCAAATGGAA AGGGGAAGGC CAAGCCCAAG GTACCAGAAA GAAAATCGTC TCTGATCTCT
    TCTATGTCCA TCTCATCATC GTCCACTTCT CTCTCTTCTA ATACTTCTAC CGAAGGAAGT
    GGGACCATGA AGAAACTGGA TACAACCCTG GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT
    CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA GACAAGTCCC CTTTTCTTCC ACCACCTCCT
    CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT CTGCCGCCCT CTCCCATGTT CCCCCCTCCA
    CCACCAGAAG CCCTTGTTCC CTTCTGCTCC CCCACCGACG GGTGCCTTTC TCCTTCTCCC
    ACAGCAGTTA GCCCCTCTCT TCCAAGATCC TTGCCTCCTG TGCCAGCACC TCCACCTTTC
    TTGCCCTCAT CAGAACCCCC GCCCGCTCCG CCTCTGGACC CCAAACTCAT GAAAGAAAAC
    AGGCCCTTTT TCAAAAACTC TAGCCAGTCC GAATCCTCTA GGGAGGCCCT CAGGCGGCCT
    GCCAACAAGG AGGAGGGCTG CCGACCCCCC ATGCCCCTGA TCACCACAGA AGCTTTGCAG
    ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA
    CCATCAGCTC AGGAACAACG AACTCCGACT GCTCCACAGT ATCACTTAAA GCCATCTGCT
    TTCCTCAAAT CCCGAAATAG CATAAATGAA ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG
    ACGAGCTCGC TTCCGATGCC TGCCAAGAGC CAGAGTCAGG GTGACCATGA CAGTGCAGTA
    GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG
    ACCACTCTGC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATCTC TAAGAAGCCC
    AAACTGTTCC TGGTGGTACC ACCTCCGCAG AGAGATTTCA CAGCGGAGCC CACAGAGAAC
    GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC CCTACCAGGG CAGAGGGGGA AGCTGTGCGG
    AGCCAAGAGG AGAAATCCAG CCCAGCATCC CGAGCTGGCT CTCATGCTAC GGCCCCCACC
    CCCGGCAGTC CGGCTCTAGA ACCGGGAACT GCAGGGTCCT TATCCTCTAG CATTGTGGAA
    GCCAATGTCC CCATGGTCCA ACCTAATACA TCACCGGGCC CCACACAGGA AGAGTCAGGC
    GAGAACAGTG TGGATGGTGA GAGAAATGCG AAGAGCTGCC TGTCTCAACA GGGCAGAGAA
    GCTGGCTTGC TGGAACCCAA CACAGCTGCT TCCTCTTCAG ACCCCGTGGA TGTATCTAAG
    GAAGAGGGAA GCGATGAGGT GCTGACTCCG ACTAAACCCA GGACAACAGA AGACCTGTTT
    GCAGCCATTC ACAGATCTAA AAGGAAAGTC CTTGGCCGGA AAGACTCAGA AGACGATCAC
    ACTCGAAATC ACTCTCCCTC CCCGCCTGTG ACACCCACGA GTGCTGCCCC TAACCTGGCC
    TCTCCAAAGC AAGTAGGGTC TATCCAGAGA AGCATAAAAA AGAGCACTAC CAGCAGTGAC
    AACTTCAAAG CTCTGCTGCT AAAGAAGGGG AGTCGCTCGG ACACCAGCGC TCGCATGTCA
    GCGGCTGAGA TGCTCAAGTC TACGGACCCT CGCTTCCAGA GATCTAGGTC GGAGCCTTCG
    GCAGACAGCC CGGACAGCCC ATCCAGCTGC TCCCCCAACA AGAACAGGAG AGCTCAGGAA
    GAGTGGGCCA AGAACGAAGG CCTGATGCCT CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC
    AGCCGCAGCA GGACGCCACC CTCTGCGGCC AGCAGCAGGT ACAGCATGCG GAACCGCATC
    CAGAGCAGCC CCATGACCGT CATCTCCGAG GGAGAGGGAG AACCTGCTGA GCCAGCGGAC
    AACAAAGCAC GCAGGGCCCT GGATGCTACC AGGGTGTGCT CTCTGGACAG ACTGACGGGT
    CAGGAGATGG ACCAGGCCAG CCTCCTCTGT AGCGAGGAGC CTGCCTCTGT GGATGGGATA
    GGAAGGGCTG AGGGCAACGG TCCCTCGGAG CAGTGTGGGG GTACGGAACA GAAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100864.1
    CDS580..5199
    Translation

    Target ORF information:

    RefSeq Version XM_030245004.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGTGGTCT TCATTAATGC AAAGATTAAA TCTTTCTTTA AACTTTTTAA AAAGAAAACG 
    GTTTCCAACT TGGACGAGGA GAGTCGATGG ACTGTCCACT ACACGGCACC ATGGCATCAG
    CAAGAGAATG TGTTCCTTCC CGCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCA AGATGGCTGC
    CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCGGCTCCAG CCCCTGCGAC
    GATTATCAAG ATGAAGACAC AGAAGCAGAC CGAAAGTGCT CTCTCTCTTC ATCTGAAGAA
    GAAAGATTTA TCGGAATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCCGACCTT
    CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACGCGG
    CAGCAAGCCC AGACGGTCCA GGCTGACGTG GTTCCTATAA ACATAACTGC ATCAGCCACT
    GGCCAAGATG ATGATGGTAG TGCCCATTCG CTGTACGTCC CAGACCACTA CTCTACGTTA
    GGAAGGCTTG ATAGTTATCG GTCCACCGGG CAATGCTTGG AAACCAGGGA CACCAGCTGT
    CAGACCGAAG ACGTGAAGGT CATCCCACCA TCAATGAGAA GGATCAGGGC TCACAAGGGG
    GTAGGCGTTG CTGCCCAGAT GAGCCACCTC TCAGGTTCCT CAGGCAACAT GTCTGTGCTG
    AGTGACTCAG CCGGTGTTGT GTTCCCATCT CGCCTCAGTA ACGATACTGG TTTCCACAGT
    CTTCCACGTA CCGGCCCGAG AGCAAGCACC TATTCACTGG AGGGAAGGAT GGGTGCCCTG
    GGCTCTACTG AAGATACGGA TGACACTTCT CCCTACCAGG GGGGTAGCTT ACAGGGGCAT
    GAAAACTTTG CGCATTTAGG AGGTGCGTCG AGTACTGGGA TGCTTTCGAG GCCCAAATCC
    CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA TGTGTGGTTT CACCTCATGC AGCCTACTCT
    ACCAGTGTCA TCCCAAATGC CACGCTACTG TCCTCTTCAG AGGTTATTGT CATTCACACT
    GCTCAGAGTG CAGGACAGCT GGACAGTAGA ACACCCGGTT CATCTTCATA TTCAAAAATA
    AAACCCAGAG ACCGTCCCAC ACCCAGATGT TCTGTGAAAG ATGACCATCA GTCCCCCCGT
    CATCACTGGA ACGAGGGTCA TCTCATTCAT TCACGGGCTT TAGCCTCCTC TGTCCCTGGT
    GCCACCACGC TGTTATCCCT CCATGATTCA GAGGTCTCTC TAAATGCCCC AGCAAACAGG
    GAAAATGGAT CCCAAGCCAT ACTCTATCAT TGTAGAAACA ACCCAAGCTT TCCTGACCAC
    CCTTCAGATG TGGATGGCAA GAGCGAGTGT AGTTATTCGG GGGACAGGGG ATGTGGCAGT
    TCTGAGCCCT GGGAATATAA AACCTCCAGC AATGGGCGGG CATCCCCACT GAAGCCACAC
    CTGGCCACTC CTGGTTGCTC TACTCCCACA AGTAACGTGA GCAGCTGCAG TTTGGACCAA
    ACATCTCTCA AGGGTGATAC CAGGTCCCTG TGTTCAGAGG ACCATGATGG TTACTATACT
    ACCACTCACG AGGCTGGGAA CCTGTACACT CTCAGTGATG GCTTGGGGAA CCCCAGGCAC
    AGCATGGTCA ATGTTTTTGA TGGAAGGGCT CAGAGAAGCC AAGGGGATCA GGCCGCTCAC
    CAGGATAAAA TCCTTTCGAG AAACATCTCT CTGAAGAAAG CAAAGAAACC ACCCCTGCCA
    CCATCCCGGA CCGATTCTCT GCGAAGGATT CCCAAAAAGA ACAACCAGAC AAACGGTCAG
    GTGCTCAATG AGAGCCTGAT CGCCTCGCTC CAGCATTCAC TGCAGCTAAG CCTCCCTGGC
    AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC TGCAGTGACT TTGAGGAACC CTGGCTCCCC
    AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG GGTAGTAGCT TGACCTCCAC CACCACTCCC
    AACGTGTATT CTCTGTGTGG GGTCACTCCG TCCCAGAGCG ACACAAGTAG CGTCAAGTCG
    GAATACACGG ACCCTTGGGG TTACTACATT GACTACACGA GCTTGCAGGA AGATCCAGGG
    AACCCCACCG GGGGCTGCTC AGCCAACACT GAGGCAGCAA CTGGAAACGG GCCAGTACGC
    CACATCCAGG AGGGGTCGAG AGTCCCAGTG CCCCAAGTGC CGGGCTGCTC AGTTAGACCA
    AAGATCGCCT CACCAGAGAA GTCACAGAGA GTCACCTCTC CATCCAGTGG GTATTCTAGC
    CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AATGTCACCA
    GCAAATGGAA AGGGGAAGGC CAAGCCCAAG GTACCAGAAA GAAAATCGTC TCTGATCTCT
    TCTATGTCCA TCTCATCATC GTCCACTTCT CTCTCTTCTA ATACTTCTAC CGAAGGAAGT
    GGGACCATGA AGAAACTGGA TACAACCCTG GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT
    CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA GACAAGTCCC CTTTTCTTCC ACCACCTCCT
    CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT CTGCCGCCCT CTCCCATGTT CCCCCCTCCA
    CCACCAGAAG CCCTTGTTCC CTTCTGCTCC CCCACCGACG GGTGCCTTTC TCCTTCTCCC
    ACAGCAGTTA GCCCCTCTCT TCCAAGATCC TTGCCTCCTG TGCCAGCACC TCCACCTTTC
    TTGCCCTCAT CAGAACCCCC GCCCGCTCCG CCTCTGGACC CCAAACTCAT GAAAGAAAAC
    AGGCCCTTTT TCAAAAACTC TAGCCAGTCC GAATCCTCTA GGGAGGCCCT CAGGCGGCCT
    GCCAACAAGG AGGAGGGCTG CCGACCCCCC ATGCCCCTGA TCACCACAGA AGCTTTGCAG
    ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA
    CCATCAGCTC AGGAACAACG AACTCCGACT GCTCCACAGT ATCACTTAAA GCCATCTGCT
    TTCCTCAAAT CCCGAAATAG CATAAATGAA ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG
    ACGAGCTCGC TTCCGATGCC TGCCAAGAGC CAGAGTCAGG GTGACCATGA CAGTGCAGTA
    GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG
    ACCACTCTGC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATCTC TAAGAAGCCC
    AAACTGTTCC TGGTGGTACC ACCTCCGCAG AGAGATTTCA CAGCGGAGCC CACAGAGAAC
    GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC CCTACCAGGG CAGAGGGGGA AGCTGTGCGG
    AGCCAAGAGG AGAAATCCAG CCCAGCATCC CGAGCTGGCT CTCATGCTAC GGCCCCCACC
    CCCGGCAGTC CGGCTCTAGA ACCGGGAACT GCAGGGTCCT TATCCTCTAG CATTGTGGAA
    GCCAATGTCC CCATGGTCCA ACCTAATACA TCACCGGGCC CCACACAGGA AGAGTCAGGC
    GAGAACAGTG TGGATGGTGA GAGAAATGCG AAGAGCTGCC TGTCTCAACA GGGCAGAGAA
    GCTGGCTTGC TGGAACCCAA CACAGCTGCT TCCTCTTCAG ACCCCGTGGA TGTATCTAAG
    GAAGAGGGAA GCGATGAGGT GCTGACTCCG ACTAAACCCA GGACAACAGA AGACCTGTTT
    GCAGCCATTC ACAGATCTAA AAGGAAAGTC CTTGGCCGGA AAGACTCAGA AGACGATCAC
    ACTCGAAATC ACTCTCCCTC CCCGCCTGTG ACACCCACGA GTGCTGCCCC TAACCTGGCC
    TCTCCAAAGC AAGTAGGGTC TATCCAGAGA AGCATAAAAA AGAGCACTAC CAGCAGTGAC
    AACTTCAAAG CTCTGCTGCT AAAGAAGGGG AGTCGCTCGG ACACCAGCGC TCGCATGTCA
    GCGGCTGAGA TGCTCAAGTC TACGGACCCT CGCTTCCAGA GATCTAGGTC GGAGCCTTCG
    GCAGACAGCC CGGACAGCCC ATCCAGCTGC TCCCCCAACA AGAACAGGAG AGCTCAGGAA
    GAGTGGGCCA AGAACGAAGG CCTGATGCCT CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC
    AGCCGCAGCA GGACGCCACC CTCTGCGGCC AGCAGCAGGT ACAGCATGCG GAACCGCATC
    CAGAGCAGCC CCATGACCGT CATCTCCGAG GGAGAGGGAG AACCTGCTGA GCCAGCGGAC
    AACAAAGCAC GCAGGGCCCT GGATGCTACC AGGGTGTGCT CTCTGGACAG ACTGACGGGT
    CAGGAGATGG ACCAGGCCAG CCTCCTCTGT AGCGAGGAGC CTGCCTCTGT GGATGGGATA
    GGAAGGGCTG AGGGCAACGG TCCCTCGGAG CAGTGTGGGG GTACGGAACA GAAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85410
    Clone ID Related Accession (Same CDS sequence) XM_030245005.1
    Accession Version XM_030245005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
    ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG
    CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC
    GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT
    CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA
    GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA
    CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC
    ATAACTGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA
    GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA
    ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG
    ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA
    GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC
    GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG
    GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG
    GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG
    CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA
    CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG
    GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA
    TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT
    GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA
    GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA
    AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC
    CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG
    GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA
    TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC
    AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC
    CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC
    TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA
    GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA
    AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC
    AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG
    CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT
    GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG
    ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC
    ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC
    TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT
    GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG
    GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA
    TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT
    TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA
    AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT
    ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT
    GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT
    TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT
    CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG
    TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG
    CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC
    AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG
    GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC
    ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG
    GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT
    CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT
    GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC
    CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC
    CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA
    GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA
    GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT
    CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA
    TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC
    ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG
    TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC
    CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG
    ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA
    GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT
    GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG
    AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC
    ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA
    TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG
    AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA
    CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT
    CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT
    GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT
    ACGGAACAGA AGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100865.1
    CDS5894..10471
    Translation

    Target ORF information:

    RefSeq Version XM_030245005.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
    ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG
    CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC
    GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT
    CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA
    GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA
    CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC
    ATAACTGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA
    GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA
    ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG
    ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA
    GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC
    GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG
    GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG
    GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG
    CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA
    CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG
    GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA
    TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT
    GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA
    GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA
    AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC
    CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG
    GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA
    TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC
    AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC
    CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC
    TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA
    GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA
    AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC
    AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG
    CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT
    GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG
    ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC
    ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC
    TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT
    GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG
    GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA
    TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT
    TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA
    AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT
    ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT
    GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT
    TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT
    CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG
    TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG
    CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC
    AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG
    GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC
    ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG
    GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT
    CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT
    GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC
    CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC
    CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA
    GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA
    GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT
    CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA
    TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC
    ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG
    TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC
    CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG
    ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA
    GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT
    GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG
    AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC
    ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA
    TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG
    AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA
    CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT
    CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT
    GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT
    ACGGAACAGA AGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu04541
    Clone ID Related Accession (Same CDS sequence) NM_173390.3
    Accession Version NM_173390.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY717318.1, AK038892.1, AC153561.5, AK043447.1 and AW488074.1. On Feb 2, 2007 this sequence version replaced NM_173390.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC
    AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA
    TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC
    CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC
    TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT
    GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT
    GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG
    TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA
    ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG
    CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT
    ATAAACATAA CTGCATCAGC CACTGGCCAA GATGATGATG GTAGTGCCCA TTCGCTGTAC
    GTCCCAGACC ACTACTCTAC GTTAGGAAGG CTTGATAGTT ATCGGTCCAC CGGGCAATGC
    TTGGAAACCA GGGACACCAG CTGTCAGACC GAAGACGTGA AGGTCATCCC ACCATCAATG
    AGAAGGATCA GGGCTCACAA GGGGGTAGGC GTTGCTGCCC AGATGAGCCA CCTCTCAGGT
    TCCTCAGGCA ACATGTCTGT GCTGAGTGAC TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC
    AGTAACGATA CTGGTTTCCA CAGTCTTCCA CGTACCGGCC CGAGAGCAAG CACCTATTCA
    CTGGAGGGAA GGATGGGTGC CCTGGGCTCT ACTGAAGATA CGGATGACAC TTCTCCCTAC
    CAGGGGGGTA GCTTACAGGG GCATGAAAAC TTTGCGCATT TAGGAGGTGC GTCGAGTACT
    GGGATGCTTT CGAGGCCCAA ATCCCAGCAG TTGAGGTTTT TGGAGAGTCC AGCATGTGTG
    GTTTCACCTC ATGCAGCCTA CTCTACCAGT GTCATCCCAA ATGCCACGCT ACTGTCCTCT
    TCAGAGGTTA TTGTCATTCA CACTGCTCAG AGTGCAGGAC AGCTGGACAG TAGAACACCC
    GGTTCATCTT CATATTCAAA AATAAAACCC AGAGACCGTC CCACACCCAG ATGTTCTGTG
    AAAGATGACC ATCAGTCCCC CCGTCATCAC TGGAACGAGG GTCATCTCAT TCATTCACGG
    GCTTTAGCCT CCTCTGTCCC TGGTGCCACC ACGCTGTTAT CCCTCCATGA TTCAGAGGTC
    TCTCTAAATG CCCCAGCAAA CAGGGAAAAT GGATCCCAAG CCATACTCTA TCATTGTAGA
    AACAACCCAA GCTTTCCTGA CCACCCTTCA GATGTGGATG GCAAGAGCGA GTGTAGTTAT
    TCGGGGGACA GGGGATGTGG CAGTTCTGAG CCCTGGGAAT ATAAAACCTC CAGCAATGGG
    CGGGCATCCC CACTGAAGCC ACACCTGGCC ACTCCTGGTT GCTCTACTCC CACAAGTAAC
    GTGAGCAGCT GCAGTTTGGA CCAAACATCT CTCAAGGGTG ATACCAGGTC CCTGTGTTCA
    GAGGACCATG ATGGTTACTA TACTACCACT CACGAGGCTG GGAACCTGTA CACTCTCAGT
    GATGGCTTGG GGAACCCCAG GCACAGCATG GTCAATGTTT TTGATGGAAG GGCTCAGAGA
    AGCCAAGGGG ATCAGGCCGC TCACCAGGAT AAAATCCTTT CGAGAAACAT CTCTCTGAAG
    AAAGCAAAGA AACCACCCCT GCCACCATCC CGGACCGATT CTCTGCGAAG GATTCCCAAA
    AAGAACAACC AGACAAACGG TCAGGTGCTC AATGAGAGCC TGATCGCCTC GCTCCAGCAT
    TCACTGCAGC TAAGCCTCCC TGGCAAGGGC GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT
    GACTTTGAGG AACCCTGGCT CCCCAGGTCC AGAAGCCAGA GCATTGTTAG CGAGGGTAGT
    AGCTTGACCT CCACCACCAC TCCCAACGTG TATTCTCTGT GTGGGGTCAC TCCGTCCCAG
    AGCGACACAA GTAGCGTCAA GTCGGAATAC ACGGACCCTT GGGGTTACTA CATTGACTAC
    ACGAGCTTGC AGGAAGATCC AGGGAACCCC ACCGGGGGCT GCTCAGCCAA CACTGAGGCA
    GCAACTGGAA ACGGGCCAGT ACGCCACATC CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA
    GTGCCGGGCT GCTCAGTTAG ACCAAAGATC GCCTCACCAG AGAAGTCACA GAGAGTCACC
    TCTCCATCCA GTGGGTATTC TAGCCAGTCG AATACACCCA CAGCACTCAC CCCTGTGCCT
    GTGTTTTTAA AATCAATGTC ACCAGCAAAT GGAAAGGGGA AGGCCAAGCC CAAGGTACCA
    GAAAGAAAAT CGTCTCTGAT CTCTTCTATG TCCATCTCAT CATCGTCCAC TTCTCTCTCT
    TCTAATACTT CTACCGAAGG AAGTGGGACC ATGAAGAAAC TGGATACAAC CCTGGCCTCG
    GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC CCTCTGCCTT CTCCGTGTCT GGCAGACAAG
    TCCCCTTTTC TTCCACCACC TCCTCCTCTA GCAGATTGCT CCGAGGGCTC TCCTCTGCCG
    CCCTCTCCCA TGTTCCCCCC TCCACCACCA GAAGCCCTTG TTCCCTTCTG CTCCCCCACC
    GACGGGTGCC TTTCTCCTTC TCCCACAGCA GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT
    CCTGTGCCAG CACCTCCACC TTTCTTGCCC TCATCAGAAC CCCCGCCCGC TCCGCCTCTG
    GACCCCAAAC TCATGAAAGA AAACAGGCCC TTTTTCAAAA ACTCTAGCCA GTCCGAATCC
    TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC AAGGAGGAGG GCTGCCGACC CCCCATGCCC
    CTGATCACCA CAGAAGCTTT GCAGATGGTG CAGTTGAGGC CAGTGAGGAA GAACTCAGGT
    GCCGGGGCAG TCCTGTTTTC TGAACCATCA GCTCAGGAAC AACGAACTCC GACTGCTCCA
    CAGTATCACT TAAAGCCATC TGCTTTCCTC AAATCCCGAA ATAGCATAAA TGAAATGGAG
    AGTGAAAGCC AGGCTGCCTC TGTGACGAGC TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT
    CAGGGTGACC ATGACAGTGC AGTAGAGCGT GGCGGCCTTC CGAGCTGCAG CGATGGAGCT
    CCAGGCCCGG GTCCCAGCCT CAGGACCACT CTGCTCCCAG ACTCTTCACC CAGCAGGAAG
    CCACCCCCCA TCTCTAAGAA GCCCAAACTG TTCCTGGTGG TACCACCTCC GCAGAGAGAT
    TTCACAGCGG AGCCCACAGA GAACGGGAGC GAAGCTTTCC CAGGCGTGCC CAGCCCTACC
    AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT
    GGCTCTCATG CTACGGCCCC CACCCCCGGC AGTCCGGCTC TAGAACCGGG AACTGCAGGG
    TCCTTATCCT CTAGCATTGT GGAAGCCAAT GTCCCCATGG TCCAACCTAA TACATCACCG
    GGCCCCACAC AGGAAGAGTC AGGCGAGAAC AGTGTGGATG GTGAGAGAAA TGCGAAGAGC
    TGCCTGTCTC AACAGGGCAG AGAAGCTGGC TTGCTGGAAC CCAACACAGC TGCTTCCTCT
    TCAGACCCCG TGGATGTATC TAAGGAAGAG GGAAGCGATG AGGTGCTGAC TCCGACTAAA
    CCCAGGACAA CAGAAGACCT GTTTGCAGCC ATTCACAGAT CTAAAAGGAA AGTCCTTGGC
    CGGAAAGACT CAGAAGACGA TCACACTCGA AATCACTCTC CCTCCCCGCC TGTGACACCC
    ACGAGTGCTG CCCCTAACCT GGCCTCTCCA AAGCAAGTAG GGTCTATCCA GAGAAGCATA
    AAAAAGAGCA CTACCAGCAG TGACAACTTC AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC
    TCGGACACCA GCGCTCGCAT GTCAGCGGCT GAGATGCTCA AGTCTACGGA CCCTCGCTTC
    CAGAGATCTA GGTCGGAGCC TTCGGCAGAC AGCCCGGACA GCCCATCCAG CTGCTCCCCC
    AACAAGAACA GGAGAGCTCA GGAAGAGTGG GCCAAGAACG AAGGCCTGAT GCCTCGGAGT
    CTGTCCTTTT CGGGCCCTCG GTACAGCCGC AGCAGGACGC CACCCTCTGC GGCCAGCAGC
    AGGTACAGCA TGCGGAACCG CATCCAGAGC AGCCCCATGA CCGTCATCTC CGAGGGAGAG
    GGAGAACCTG CTGAGCCAGC GGACAACAAA GCACGCAGGG CCCTGGATGC TACCAGGGTG
    TGCTCTCTGG ACAGACTGAC GGGTCAGGAG ATGGACCAGG CCAGCCTCCT CTGTAGCGAG
    GAGCCTGCCT CTGTGGATGG GATAGGAAGG GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT
    GGGGGTACGG AACAGAAGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_775566.3
    CDS639..5402
    Translation

    Target ORF information:

    RefSeq Version NM_173390.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_173390.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC
    AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA
    TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC
    CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC
    TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT
    GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT
    GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG
    TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA
    ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG
    CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT
    ATAAACATAA CTGCATCAGC CACTGGCCAA GATGATGATG GTAGTGCCCA TTCGCTGTAC
    GTCCCAGACC ACTACTCTAC GTTAGGAAGG CTTGATAGTT ATCGGTCCAC CGGGCAATGC
    TTGGAAACCA GGGACACCAG CTGTCAGACC GAAGACGTGA AGGTCATCCC ACCATCAATG
    AGAAGGATCA GGGCTCACAA GGGGGTAGGC GTTGCTGCCC AGATGAGCCA CCTCTCAGGT
    TCCTCAGGCA ACATGTCTGT GCTGAGTGAC TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC
    AGTAACGATA CTGGTTTCCA CAGTCTTCCA CGTACCGGCC CGAGAGCAAG CACCTATTCA
    CTGGAGGGAA GGATGGGTGC CCTGGGCTCT ACTGAAGATA CGGATGACAC TTCTCCCTAC
    CAGGGGGGTA GCTTACAGGG GCATGAAAAC TTTGCGCATT TAGGAGGTGC GTCGAGTACT
    GGGATGCTTT CGAGGCCCAA ATCCCAGCAG TTGAGGTTTT TGGAGAGTCC AGCATGTGTG
    GTTTCACCTC ATGCAGCCTA CTCTACCAGT GTCATCCCAA ATGCCACGCT ACTGTCCTCT
    TCAGAGGTTA TTGTCATTCA CACTGCTCAG AGTGCAGGAC AGCTGGACAG TAGAACACCC
    GGTTCATCTT CATATTCAAA AATAAAACCC AGAGACCGTC CCACACCCAG ATGTTCTGTG
    AAAGATGACC ATCAGTCCCC CCGTCATCAC TGGAACGAGG GTCATCTCAT TCATTCACGG
    GCTTTAGCCT CCTCTGTCCC TGGTGCCACC ACGCTGTTAT CCCTCCATGA TTCAGAGGTC
    TCTCTAAATG CCCCAGCAAA CAGGGAAAAT GGATCCCAAG CCATACTCTA TCATTGTAGA
    AACAACCCAA GCTTTCCTGA CCACCCTTCA GATGTGGATG GCAAGAGCGA GTGTAGTTAT
    TCGGGGGACA GGGGATGTGG CAGTTCTGAG CCCTGGGAAT ATAAAACCTC CAGCAATGGG
    CGGGCATCCC CACTGAAGCC ACACCTGGCC ACTCCTGGTT GCTCTACTCC CACAAGTAAC
    GTGAGCAGCT GCAGTTTGGA CCAAACATCT CTCAAGGGTG ATACCAGGTC CCTGTGTTCA
    GAGGACCATG ATGGTTACTA TACTACCACT CACGAGGCTG GGAACCTGTA CACTCTCAGT
    GATGGCTTGG GGAACCCCAG GCACAGCATG GTCAATGTTT TTGATGGAAG GGCTCAGAGA
    AGCCAAGGGG ATCAGGCCGC TCACCAGGAT AAAATCCTTT CGAGAAACAT CTCTCTGAAG
    AAAGCAAAGA AACCACCCCT GCCACCATCC CGGACCGATT CTCTGCGAAG GATTCCCAAA
    AAGAACAACC AGACAAACGG TCAGGTGCTC AATGAGAGCC TGATCGCCTC GCTCCAGCAT
    TCACTGCAGC TAAGCCTCCC TGGCAAGGGC GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT
    GACTTTGAGG AACCCTGGCT CCCCAGGTCC AGAAGCCAGA GCATTGTTAG CGAGGGTAGT
    AGCTTGACCT CCACCACCAC TCCCAACGTG TATTCTCTGT GTGGGGTCAC TCCGTCCCAG
    AGCGACACAA GTAGCGTCAA GTCGGAATAC ACGGACCCTT GGGGTTACTA CATTGACTAC
    ACGAGCTTGC AGGAAGATCC AGGGAACCCC ACCGGGGGCT GCTCAGCCAA CACTGAGGCA
    GCAACTGGAA ACGGGCCAGT ACGCCACATC CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA
    GTGCCGGGCT GCTCAGTTAG ACCAAAGATC GCCTCACCAG AGAAGTCACA GAGAGTCACC
    TCTCCATCCA GTGGGTATTC TAGCCAGTCG AATACACCCA CAGCACTCAC CCCTGTGCCT
    GTGTTTTTAA AATCAATGTC ACCAGCAAAT GGAAAGGGGA AGGCCAAGCC CAAGGTACCA
    GAAAGAAAAT CGTCTCTGAT CTCTTCTATG TCCATCTCAT CATCGTCCAC TTCTCTCTCT
    TCTAATACTT CTACCGAAGG AAGTGGGACC ATGAAGAAAC TGGATACAAC CCTGGCCTCG
    GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC CCTCTGCCTT CTCCGTGTCT GGCAGACAAG
    TCCCCTTTTC TTCCACCACC TCCTCCTCTA GCAGATTGCT CCGAGGGCTC TCCTCTGCCG
    CCCTCTCCCA TGTTCCCCCC TCCACCACCA GAAGCCCTTG TTCCCTTCTG CTCCCCCACC
    GACGGGTGCC TTTCTCCTTC TCCCACAGCA GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT
    CCTGTGCCAG CACCTCCACC TTTCTTGCCC TCATCAGAAC CCCCGCCCGC TCCGCCTCTG
    GACCCCAAAC TCATGAAAGA AAACAGGCCC TTTTTCAAAA ACTCTAGCCA GTCCGAATCC
    TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC AAGGAGGAGG GCTGCCGACC CCCCATGCCC
    CTGATCACCA CAGAAGCTTT GCAGATGGTG CAGTTGAGGC CAGTGAGGAA GAACTCAGGT
    GCCGGGGCAG TCCTGTTTTC TGAACCATCA GCTCAGGAAC AACGAACTCC GACTGCTCCA
    CAGTATCACT TAAAGCCATC TGCTTTCCTC AAATCCCGAA ATAGCATAAA TGAAATGGAG
    AGTGAAAGCC AGGCTGCCTC TGTGACGAGC TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT
    CAGGGTGACC ATGACAGTGC AGTAGAGCGT GGCGGCCTTC CGAGCTGCAG CGATGGAGCT
    CCAGGCCCGG GTCCCAGCCT CAGGACCACT CTGCTCCCAG ACTCTTCACC CAGCAGGAAG
    CCACCCCCCA TCTCTAAGAA GCCCAAACTG TTCCTGGTGG TACCACCTCC GCAGAGAGAT
    TTCACAGCGG AGCCCACAGA GAACGGGAGC GAAGCTTTCC CAGGCGTGCC CAGCCCTACC
    AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT
    GGCTCTCATG CTACGGCCCC CACCCCCGGC AGTCCGGCTC TAGAACCGGG AACTGCAGGG
    TCCTTATCCT CTAGCATTGT GGAAGCCAAT GTCCCCATGG TCCAACCTAA TACATCACCG
    GGCCCCACAC AGGAAGAGTC AGGCGAGAAC AGTGTGGATG GTGAGAGAAA TGCGAAGAGC
    TGCCTGTCTC AACAGGGCAG AGAAGCTGGC TTGCTGGAAC CCAACACAGC TGCTTCCTCT
    TCAGACCCCG TGGATGTATC TAAGGAAGAG GGAAGCGATG AGGTGCTGAC TCCGACTAAA
    CCCAGGACAA CAGAAGACCT GTTTGCAGCC ATTCACAGAT CTAAAAGGAA AGTCCTTGGC
    CGGAAAGACT CAGAAGACGA TCACACTCGA AATCACTCTC CCTCCCCGCC TGTGACACCC
    ACGAGTGCTG CCCCTAACCT GGCCTCTCCA AAGCAAGTAG GGTCTATCCA GAGAAGCATA
    AAAAAGAGCA CTACCAGCAG TGACAACTTC AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC
    TCGGACACCA GCGCTCGCAT GTCAGCGGCT GAGATGCTCA AGTCTACGGA CCCTCGCTTC
    CAGAGATCTA GGTCGGAGCC TTCGGCAGAC AGCCCGGACA GCCCATCCAG CTGCTCCCCC
    AACAAGAACA GGAGAGCTCA GGAAGAGTGG GCCAAGAACG AAGGCCTGAT GCCTCGGAGT
    CTGTCCTTTT CGGGCCCTCG GTACAGCCGC AGCAGGACGC CACCCTCTGC GGCCAGCAGC
    AGGTACAGCA TGCGGAACCG CATCCAGAGC AGCCCCATGA CCGTCATCTC CGAGGGAGAG
    GGAGAACCTG CTGAGCCAGC GGACAACAAA GCACGCAGGG CCCTGGATGC TACCAGGGTG
    TGCTCTCTGG ACAGACTGAC GGGTCAGGAG ATGGACCAGG CCAGCCTCCT CTGTAGCGAG
    GAGCCTGCCT CTGTGGATGG GATAGGAAGG GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT
    GGGGGTACGG AACAGAAGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu09232
    Clone ID Related Accession (Same CDS sequence) NM_001163592.1
    Accession Version NM_001163592.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153561.5 and AW488074.1. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, uses a distinct start codon, and includes an alternate in-frame exon, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus and includes an alternate segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGGTCT TCATTAATGC AAAGATTAAA TCTTTCTTTA AACTTTTTAA AAAGAAAACG 
    GTTTCCAACT TGGACGAGGA GAGTCGATGG ACTGTCCACT ACACGGCACC ATGGCATCAG
    CAAGAGAATG TGTTCCTTCC CGCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCA AGATGGCTGC
    CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCGGCTCCAG CCCCTGCGAC
    GATTATCAAG ATGAAGACAC AGAAGCAGAC CGAAAGTGCT CTCTCTCTTC ATCTGAAGAA
    GAAAGATTTA TCGGAATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCCGACCTT
    CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACGCGG
    CAGCAAGCCC AGACGGTCCA GGCTGACGTG GTTCCTATAA ACATAACTGG GGAGAATTTC
    GATCGTCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACAG ACACTCTGGT AAGACGACCG
    AAGAAAGTCA AAAGGAGAAA GACAATTTCA GGAATCCCTG ACATCATTCA GAAGGAGCTA
    GCATCAGCCA CTGGCCAAGA TGATGATGGT AGTGCCCATT CGCTGTACGT CCCAGACCAC
    TACTCTACGT TAGGAAGGCT TGATAGTTAT CGGTCCACCG GGCAATGCTT GGAAACCAGG
    GACACCAGCT GTCAGACCGA AGACGTGAAG GTCATCCCAC CATCAATGAG AAGGATCAGG
    GCTCACAAGG GGGTAGGCGT TGCTGCCCAG ATGAGCCACC TCTCAGGTTC CTCAGGCAAC
    ATGTCTGTGC TGAGTGACTC AGCCGGTGTT GTGTTCCCAT CTCGCCTCAG TAACGATACT
    GGTTTCCACA GTCTTCCACG TACCGGCCCG AGAGCAAGCA CCTATTCACT GGAGGGAAGG
    ATGGGTGCCC TGGGCTCTAC TGAAGATACG GATGACACTT CTCCCTACCA GGGGGGTAGC
    TTACAGGGGC ATGAAAACTT TGCGCATTTA GGAGGTGCGT CGAGTACTGG GATGCTTTCG
    AGGCCCAAAT CCCAGCAGTT GAGGTTTTTG GAGAGTCCAG CATGTGTGGT TTCACCTCAT
    GCAGCCTACT CTACCAGTGT CATCCCAAAT GCCACGCTAC TGTCCTCTTC AGAGGTTATT
    GTCATTCACA CTGCTCAGAG TGCAGGACAG CTGGACAGTA GAACACCCGG TTCATCTTCA
    TATTCAAAAA TAAAACCCAG AGACCGTCCC ACACCCAGAT GTTCTGTGAA AGATGACCAT
    CAGTCCCCCC GTCATCACTG GAACGAGGGT CATCTCATTC ATTCACGGGC TTTAGCCTCC
    TCTGTCCCTG GTGCCACCAC GCTGTTATCC CTCCATGATT CAGAGGTCTC TCTAAATGCC
    CCAGCAAACA GGGAAAATGG ATCCCAAGCC ATACTCTATC ATTGTAGAAA CAACCCAAGC
    TTTCCTGACC ACCCTTCAGA TGTGGATGGC AAGAGCGAGT GTAGTTATTC GGGGGACAGG
    GGATGTGGCA GTTCTGAGCC CTGGGAATAT AAAACCTCCA GCAATGGGCG GGCATCCCCA
    CTGAAGCCAC ACCTGGCCAC TCCTGGTTGC TCTACTCCCA CAAGTAACGT GAGCAGCTGC
    AGTTTGGACC AAACATCTCT CAAGGGTGAT ACCAGGTCCC TGTGTTCAGA GGACCATGAT
    GGTTACTATA CTACCACTCA CGAGGCTGGG AACCTGTACA CTCTCAGTGA TGGCTTGGGG
    AACCCCAGGC ACAGCATGGT CAATGTTTTT GATGGAAGGG CTCAGAGAAG CCAAGGGGAT
    CAGGCCGCTC ACCAGGATAA AATCCTTTCG AGAAACATCT CTCTGAAGAA AGCAAAGAAA
    CCACCCCTGC CACCATCCCG GACCGATTCT CTGCGAAGGA TTCCCAAAAA GAACAACCAG
    ACAAACGGTC AGGTGCTCAA TGAGAGCCTG ATCGCCTCGC TCCAGCATTC ACTGCAGCTA
    AGCCTCCCTG GCAAGGGCGG CAGCTCCCCT TCCCAGAGCC CCTGCAGTGA CTTTGAGGAA
    CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC ATTGTTAGCG AGGGTAGTAG CTTGACCTCC
    ACCACCACTC CCAACGTGTA TTCTCTGTGT GGGGTCACTC CGTCCCAGAG CGACACAAGT
    AGCGTCAAGT CGGAATACAC GGACCCTTGG GGTTACTACA TTGACTACAC GAGCTTGCAG
    GAAGATCCAG GGAACCCCAC CGGGGGCTGC TCAGCCAACA CTGAGGCAGC AACTGGAAAC
    GGGCCAGTAC GCCACATCCA GGAGGGGTCG AGAGTCCCAG TGCCCCAAGT GCCGGGCTGC
    TCAGTTAGAC CAAAGATCGC CTCACCAGAG AAGTCACAGA GAGTCACCTC TCCATCCAGT
    GGGTATTCTA GCCAGTCGAA TACACCCACA GCACTCACCC CTGTGCCTGT GTTTTTAAAA
    TCAATGTCAC CAGCAAATGG AAAGGGGAAG GCCAAGCCCA AGGTACCAGA AAGAAAATCG
    TCTCTGATCT CTTCTATGTC CATCTCATCA TCGTCCACTT CTCTCTCTTC TAATACTTCT
    ACCGAAGGAA GTGGGACCAT GAAGAAACTG GATACAACCC TGGCCTCGGC CCTTGCCCCT
    CCCCCTCCTC CTCTTCCCCC TCTGCCTTCT CCGTGTCTGG CAGACAAGTC CCCTTTTCTT
    CCACCACCTC CTCCTCTAGC AGATTGCTCC GAGGGCTCTC CTCTGCCGCC CTCTCCCATG
    TTCCCCCCTC CACCACCAGA AGCCCTTGTT CCCTTCTGCT CCCCCACCGA CGGGTGCCTT
    TCTCCTTCTC CCACAGCAGT TAGCCCCTCT CTTCCAAGAT CCTTGCCTCC TGTGCCAGCA
    CCTCCACCTT TCTTGCCCTC ATCAGAACCC CCGCCCGCTC CGCCTCTGGA CCCCAAACTC
    ATGAAAGAAA ACAGGCCCTT TTTCAAAAAC TCTAGCCAGT CCGAATCCTC TAGGGAGGCC
    CTCAGGCGGC CTGCCAACAA GGAGGAGGGC TGCCGACCCC CCATGCCCCT GATCACCACA
    GAAGCTTTGC AGATGGTGCA GTTGAGGCCA GTGAGGAAGA ACTCAGGTGC CGGGGCAGTC
    CTGTTTTCTG AACCATCAGC TCAGGAACAA CGAACTCCGA CTGCTCCACA GTATCACTTA
    AAGCCATCTG CTTTCCTCAA ATCCCGAAAT AGCATAAATG AAATGGAGAG TGAAAGCCAG
    GCTGCCTCTG TGACGAGCTC GCTTCCGATG CCTGCCAAGA GCCAGAGTCA GGGTGACCAT
    GACAGTGCAG TAGAGCGTGG CGGCCTTCCG AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT
    CCCAGCCTCA GGACCACTCT GCTCCCAGAC TCTTCACCCA GCAGGAAGCC ACCCCCCATC
    TCTAAGAAGC CCAAACTGTT CCTGGTGGTA CCACCTCCGC AGAGAGATTT CACAGCGGAG
    CCCACAGAGA ACGGGAGCGA AGCTTTCCCA GGCGTGCCCA GCCCTACCAG GGCAGAGGGG
    GAAGCTGTGC GGAGCCAAGA GGAGAAATCC AGCCCAGCAT CCCGAGCTGG CTCTCATGCT
    ACGGCCCCCA CCCCCGGCAG TCCGGCTCTA GAACCGGGAA CTGCAGGGTC CTTATCCTCT
    AGCATTGTGG AAGCCAATGT CCCCATGGTC CAACCTAATA CATCACCGGG CCCCACACAG
    GAAGAGTCAG GCGAGAACAG TGTGGATGGT GAGAGAAATG CGAAGAGCTG CCTGTCTCAA
    CAGGGCAGAG AAGCTGGCTT GCTGGAACCC AACACAGCTG CTTCCTCTTC AGACCCCGTG
    GATGTATCTA AGGAAGAGGG AAGCGATGAG GTGCTGACTC CGACTAAACC CAGGACAACA
    GAAGACCTGT TTGCAGCCAT TCACAGATCT AAAAGGAAAG TCCTTGGCCG GAAAGACTCA
    GAAGACGATC ACACTCGAAA TCACTCTCCC TCCCCGCCTG TGACACCCAC GAGTGCTGCC
    CCTAACCTGG CCTCTCCAAA GCAAGTAGGG TCTATCCAGA GAAGCATAAA AAAGAGCACT
    ACCAGCAGTG ACAACTTCAA AGCTCTGCTG CTAAAGAAGG GGAGTCGCTC GGACACCAGC
    GCTCGCATGT CAGCGGCTGA GATGCTCAAG TCTACGGACC CTCGCTTCCA GAGATCTAGG
    TCGGAGCCTT CGGCAGACAG CCCGGACAGC CCATCCAGCT GCTCCCCCAA CAAGAACAGG
    AGAGCTCAGG AAGAGTGGGC CAAGAACGAA GGCCTGATGC CTCGGAGTCT GTCCTTTTCG
    GGCCCTCGGT ACAGCCGCAG CAGGACGCCA CCCTCTGCGG CCAGCAGCAG GTACAGCATG
    CGGAACCGCA TCCAGAGCAG CCCCATGACC GTCATCTCCG AGGGAGAGGG AGAACCTGCT
    GAGCCAGCGG ACAACAAAGC ACGCAGGGCC CTGGATGCTA CCAGGGTGTG CTCTCTGGAC
    AGACTGACGG GTCAGGAGAT GGACCAGGCC AGCCTCCTCT GTAGCGAGGA GCCTGCCTCT
    GTGGATGGGA TAGGAAGGGC TGAGGGCAAC GGTCCCTCGG AGCAGTGTGG GGGTACGGAA
    CAGAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157064.1
    CDS128..4879
    Translation

    Target ORF information:

    RefSeq Version NM_001163592.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163592.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGGTCT TCATTAATGC AAAGATTAAA TCTTTCTTTA AACTTTTTAA AAAGAAAACG 
    GTTTCCAACT TGGACGAGGA GAGTCGATGG ACTGTCCACT ACACGGCACC ATGGCATCAG
    CAAGAGAATG TGTTCCTTCC CGCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA
    GCCAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCA AGATGGCTGC
    CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCGGCTCCAG CCCCTGCGAC
    GATTATCAAG ATGAAGACAC AGAAGCAGAC CGAAAGTGCT CTCTCTCTTC ATCTGAAGAA
    GAAAGATTTA TCGGAATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCCGACCTT
    CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACGCGG
    CAGCAAGCCC AGACGGTCCA GGCTGACGTG GTTCCTATAA ACATAACTGG GGAGAATTTC
    GATCGTCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACAG ACACTCTGGT AAGACGACCG
    AAGAAAGTCA AAAGGAGAAA GACAATTTCA GGAATCCCTG ACATCATTCA GAAGGAGCTA
    GCATCAGCCA CTGGCCAAGA TGATGATGGT AGTGCCCATT CGCTGTACGT CCCAGACCAC
    TACTCTACGT TAGGAAGGCT TGATAGTTAT CGGTCCACCG GGCAATGCTT GGAAACCAGG
    GACACCAGCT GTCAGACCGA AGACGTGAAG GTCATCCCAC CATCAATGAG AAGGATCAGG
    GCTCACAAGG GGGTAGGCGT TGCTGCCCAG ATGAGCCACC TCTCAGGTTC CTCAGGCAAC
    ATGTCTGTGC TGAGTGACTC AGCCGGTGTT GTGTTCCCAT CTCGCCTCAG TAACGATACT
    GGTTTCCACA GTCTTCCACG TACCGGCCCG AGAGCAAGCA CCTATTCACT GGAGGGAAGG
    ATGGGTGCCC TGGGCTCTAC TGAAGATACG GATGACACTT CTCCCTACCA GGGGGGTAGC
    TTACAGGGGC ATGAAAACTT TGCGCATTTA GGAGGTGCGT CGAGTACTGG GATGCTTTCG
    AGGCCCAAAT CCCAGCAGTT GAGGTTTTTG GAGAGTCCAG CATGTGTGGT TTCACCTCAT
    GCAGCCTACT CTACCAGTGT CATCCCAAAT GCCACGCTAC TGTCCTCTTC AGAGGTTATT
    GTCATTCACA CTGCTCAGAG TGCAGGACAG CTGGACAGTA GAACACCCGG TTCATCTTCA
    TATTCAAAAA TAAAACCCAG AGACCGTCCC ACACCCAGAT GTTCTGTGAA AGATGACCAT
    CAGTCCCCCC GTCATCACTG GAACGAGGGT CATCTCATTC ATTCACGGGC TTTAGCCTCC
    TCTGTCCCTG GTGCCACCAC GCTGTTATCC CTCCATGATT CAGAGGTCTC TCTAAATGCC
    CCAGCAAACA GGGAAAATGG ATCCCAAGCC ATACTCTATC ATTGTAGAAA CAACCCAAGC
    TTTCCTGACC ACCCTTCAGA TGTGGATGGC AAGAGCGAGT GTAGTTATTC GGGGGACAGG
    GGATGTGGCA GTTCTGAGCC CTGGGAATAT AAAACCTCCA GCAATGGGCG GGCATCCCCA
    CTGAAGCCAC ACCTGGCCAC TCCTGGTTGC TCTACTCCCA CAAGTAACGT GAGCAGCTGC
    AGTTTGGACC AAACATCTCT CAAGGGTGAT ACCAGGTCCC TGTGTTCAGA GGACCATGAT
    GGTTACTATA CTACCACTCA CGAGGCTGGG AACCTGTACA CTCTCAGTGA TGGCTTGGGG
    AACCCCAGGC ACAGCATGGT CAATGTTTTT GATGGAAGGG CTCAGAGAAG CCAAGGGGAT
    CAGGCCGCTC ACCAGGATAA AATCCTTTCG AGAAACATCT CTCTGAAGAA AGCAAAGAAA
    CCACCCCTGC CACCATCCCG GACCGATTCT CTGCGAAGGA TTCCCAAAAA GAACAACCAG
    ACAAACGGTC AGGTGCTCAA TGAGAGCCTG ATCGCCTCGC TCCAGCATTC ACTGCAGCTA
    AGCCTCCCTG GCAAGGGCGG CAGCTCCCCT TCCCAGAGCC CCTGCAGTGA CTTTGAGGAA
    CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC ATTGTTAGCG AGGGTAGTAG CTTGACCTCC
    ACCACCACTC CCAACGTGTA TTCTCTGTGT GGGGTCACTC CGTCCCAGAG CGACACAAGT
    AGCGTCAAGT CGGAATACAC GGACCCTTGG GGTTACTACA TTGACTACAC GAGCTTGCAG
    GAAGATCCAG GGAACCCCAC CGGGGGCTGC TCAGCCAACA CTGAGGCAGC AACTGGAAAC
    GGGCCAGTAC GCCACATCCA GGAGGGGTCG AGAGTCCCAG TGCCCCAAGT GCCGGGCTGC
    TCAGTTAGAC CAAAGATCGC CTCACCAGAG AAGTCACAGA GAGTCACCTC TCCATCCAGT
    GGGTATTCTA GCCAGTCGAA TACACCCACA GCACTCACCC CTGTGCCTGT GTTTTTAAAA
    TCAATGTCAC CAGCAAATGG AAAGGGGAAG GCCAAGCCCA AGGTACCAGA AAGAAAATCG
    TCTCTGATCT CTTCTATGTC CATCTCATCA TCGTCCACTT CTCTCTCTTC TAATACTTCT
    ACCGAAGGAA GTGGGACCAT GAAGAAACTG GATACAACCC TGGCCTCGGC CCTTGCCCCT
    CCCCCTCCTC CTCTTCCCCC TCTGCCTTCT CCGTGTCTGG CAGACAAGTC CCCTTTTCTT
    CCACCACCTC CTCCTCTAGC AGATTGCTCC GAGGGCTCTC CTCTGCCGCC CTCTCCCATG
    TTCCCCCCTC CACCACCAGA AGCCCTTGTT CCCTTCTGCT CCCCCACCGA CGGGTGCCTT
    TCTCCTTCTC CCACAGCAGT TAGCCCCTCT CTTCCAAGAT CCTTGCCTCC TGTGCCAGCA
    CCTCCACCTT TCTTGCCCTC ATCAGAACCC CCGCCCGCTC CGCCTCTGGA CCCCAAACTC
    ATGAAAGAAA ACAGGCCCTT TTTCAAAAAC TCTAGCCAGT CCGAATCCTC TAGGGAGGCC
    CTCAGGCGGC CTGCCAACAA GGAGGAGGGC TGCCGACCCC CCATGCCCCT GATCACCACA
    GAAGCTTTGC AGATGGTGCA GTTGAGGCCA GTGAGGAAGA ACTCAGGTGC CGGGGCAGTC
    CTGTTTTCTG AACCATCAGC TCAGGAACAA CGAACTCCGA CTGCTCCACA GTATCACTTA
    AAGCCATCTG CTTTCCTCAA ATCCCGAAAT AGCATAAATG AAATGGAGAG TGAAAGCCAG
    GCTGCCTCTG TGACGAGCTC GCTTCCGATG CCTGCCAAGA GCCAGAGTCA GGGTGACCAT
    GACAGTGCAG TAGAGCGTGG CGGCCTTCCG AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT
    CCCAGCCTCA GGACCACTCT GCTCCCAGAC TCTTCACCCA GCAGGAAGCC ACCCCCCATC
    TCTAAGAAGC CCAAACTGTT CCTGGTGGTA CCACCTCCGC AGAGAGATTT CACAGCGGAG
    CCCACAGAGA ACGGGAGCGA AGCTTTCCCA GGCGTGCCCA GCCCTACCAG GGCAGAGGGG
    GAAGCTGTGC GGAGCCAAGA GGAGAAATCC AGCCCAGCAT CCCGAGCTGG CTCTCATGCT
    ACGGCCCCCA CCCCCGGCAG TCCGGCTCTA GAACCGGGAA CTGCAGGGTC CTTATCCTCT
    AGCATTGTGG AAGCCAATGT CCCCATGGTC CAACCTAATA CATCACCGGG CCCCACACAG
    GAAGAGTCAG GCGAGAACAG TGTGGATGGT GAGAGAAATG CGAAGAGCTG CCTGTCTCAA
    CAGGGCAGAG AAGCTGGCTT GCTGGAACCC AACACAGCTG CTTCCTCTTC AGACCCCGTG
    GATGTATCTA AGGAAGAGGG AAGCGATGAG GTGCTGACTC CGACTAAACC CAGGACAACA
    GAAGACCTGT TTGCAGCCAT TCACAGATCT AAAAGGAAAG TCCTTGGCCG GAAAGACTCA
    GAAGACGATC ACACTCGAAA TCACTCTCCC TCCCCGCCTG TGACACCCAC GAGTGCTGCC
    CCTAACCTGG CCTCTCCAAA GCAAGTAGGG TCTATCCAGA GAAGCATAAA AAAGAGCACT
    ACCAGCAGTG ACAACTTCAA AGCTCTGCTG CTAAAGAAGG GGAGTCGCTC GGACACCAGC
    GCTCGCATGT CAGCGGCTGA GATGCTCAAG TCTACGGACC CTCGCTTCCA GAGATCTAGG
    TCGGAGCCTT CGGCAGACAG CCCGGACAGC CCATCCAGCT GCTCCCCCAA CAAGAACAGG
    AGAGCTCAGG AAGAGTGGGC CAAGAACGAA GGCCTGATGC CTCGGAGTCT GTCCTTTTCG
    GGCCCTCGGT ACAGCCGCAG CAGGACGCCA CCCTCTGCGG CCAGCAGCAG GTACAGCATG
    CGGAACCGCA TCCAGAGCAG CCCCATGACC GTCATCTCCG AGGGAGAGGG AGAACCTGCT
    GAGCCAGCGG ACAACAAAGC ACGCAGGGCC CTGGATGCTA CCAGGGTGTG CTCTCTGGAC
    AGACTGACGG GTCAGGAGAT GGACCAGGCC AGCCTCCTCT GTAGCGAGGA GCCTGCCTCT
    GTGGATGGGA TAGGAAGGGC TGAGGGCAAC GGTCCCTCGG AGCAGTGTGG GGGTACGGAA
    CAGAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43843
    Clone ID Related Accession (Same CDS sequence) XM_006512686.3 , XM_006512686.4
    Accession Version XM_006512686.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512686.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
    CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC
    CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG
    GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG
    GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC
    AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG
    AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG
    CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT
    TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT
    CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA
    TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT
    CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA
    GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGGGGAGAA TTTCGATCGT
    CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACAGACACTC TGGTAAGACG ACCGAAGAAA
    GTCAAAAGGA GAAAGACAAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCATCA
    GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT
    ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC
    AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC
    AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT
    GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC
    CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT
    GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG
    GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC
    AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC
    TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT
    CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA
    AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC
    CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC
    CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA
    AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT
    GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT
    GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG
    CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG
    GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC
    TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC
    AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC
    GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC
    CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC
    GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC
    CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG
    CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC
    ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC
    AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT
    CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA
    GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT
    AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT
    TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG
    TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG
    ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA
    GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT
    CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA
    CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC
    CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT
    TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA
    CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA
    GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG
    CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT
    TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT
    TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA
    TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC
    TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT
    GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC
    CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG
    AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA
    GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT
    GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC
    CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT
    GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG
    TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC
    AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA
    TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC
    CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC
    GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC
    CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC
    ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG
    CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT
    CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT
    CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC
    CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA
    GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG
    ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT
    GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512749.1
    CDS424..5409
    Translation

    Target ORF information:

    RefSeq Version XM_006512686.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512686.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
    CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC
    CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG
    GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG
    GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC
    AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG
    AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG
    CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT
    TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT
    CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA
    TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT
    CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA
    GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGGGGAGAA TTTCGATCGT
    CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACAGACACTC TGGTAAGACG ACCGAAGAAA
    GTCAAAAGGA GAAAGACAAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCATCA
    GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT
    ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC
    AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC
    AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT
    GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC
    CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT
    GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG
    GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC
    AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC
    TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT
    CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA
    AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC
    CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC
    CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA
    AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT
    GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT
    GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG
    CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG
    GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC
    TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC
    AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC
    GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC
    CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC
    GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC
    CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG
    CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC
    ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC
    AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT
    CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA
    GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT
    AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT
    TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG
    TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG
    ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA
    GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT
    CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA
    CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC
    CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT
    TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA
    CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA
    GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG
    CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT
    TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT
    TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA
    TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC
    TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT
    GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC
    CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG
    AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA
    GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT
    GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC
    CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT
    GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG
    TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC
    AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA
    TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC
    CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC
    GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC
    CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC
    AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC
    ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG
    CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT
    CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT
    CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC
    CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA
    GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG
    ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT
    GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43844
    Clone ID Related Accession (Same CDS sequence) XM_006512687.3 , XM_006512687.4
    Accession Version XM_006512687.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC
    AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA
    TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC
    CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC
    TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT
    GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT
    GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG
    TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA
    ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG
    CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT
    ATAAACATAA CTGGGGAGAA TTTCGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC
    ACAGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACAAT TTCAGGAATC
    CCTGACATCA TTCAGAAGGA GCTAGCATCA GCCACTGGCC AAGATGATGA TGGTAGTGCC
    CATTCGCTGT ACGTCCCAGA CCACTACTCT ACGTTAGGAA GGCTTGATAG TTATCGGTCC
    ACCGGGCAAT GCTTGGAAAC CAGGGACACC AGCTGTCAGA CCGAAGACGT GAAGGTCATC
    CCACCATCAA TGAGAAGGAT CAGGGCTCAC AAGGGGGTAG GCGTTGCTGC CCAGATGAGC
    CACCTCTCAG GTTCCTCAGG CAACATGTCT GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC
    CCATCTCGCC TCAGTAACGA TACTGGTTTC CACAGTCTTC CACGTACCGG CCCGAGAGCA
    AGCACCTATT CACTGGAGGG AAGGATGGGT GCCCTGGGCT CTACTGAAGA TACGGATGAC
    ACTTCTCCCT ACCAGGGGGG TAGCTTACAG GGGCATGAAA ACTTTGCGCA TTTAGGAGGT
    GCGTCGAGTA CTGGGATGCT TTCGAGGCCC AAATCCCAGC AGTTGAGGTT TTTGGAGAGT
    CCAGCATGTG TGGTTTCACC TCATGCAGCC TACTCTACCA GTGTCATCCC AAATGCCACG
    CTACTGTCCT CTTCAGAGGT TATTGTCATT CACACTGCTC AGAGTGCAGG ACAGCTGGAC
    AGTAGAACAC CCGGTTCATC TTCATATTCA AAAATAAAAC CCAGAGACCG TCCCACACCC
    AGATGTTCTG TGAAAGATGA CCATCAGTCC CCCCGTCATC ACTGGAACGA GGGTCATCTC
    ATTCATTCAC GGGCTTTAGC CTCCTCTGTC CCTGGTGCCA CCACGCTGTT ATCCCTCCAT
    GATTCAGAGG TCTCTCTAAA TGCCCCAGCA AACAGGGAAA ATGGATCCCA AGCCATACTC
    TATCATTGTA GAAACAACCC AAGCTTTCCT GACCACCCTT CAGATGTGGA TGGCAAGAGC
    GAGTGTAGTT ATTCGGGGGA CAGGGGATGT GGCAGTTCTG AGCCCTGGGA ATATAAAACC
    TCCAGCAATG GGCGGGCATC CCCACTGAAG CCACACCTGG CCACTCCTGG TTGCTCTACT
    CCCACAAGTA ACGTGAGCAG CTGCAGTTTG GACCAAACAT CTCTCAAGGG TGATACCAGG
    TCCCTGTGTT CAGAGGACCA TGATGGTTAC TATACTACCA CTCACGAGGC TGGGAACCTG
    TACACTCTCA GTGATGGCTT GGGGAACCCC AGGCACAGCA TGGTCAATGT TTTTGATGGA
    AGGGCTCAGA GAAGCCAAGG GGATCAGGCC GCTCACCAGG ATAAAATCCT TTCGAGAAAC
    ATCTCTCTGA AGAAAGCAAA GAAACCACCC CTGCCACCAT CCCGGACCGA TTCTCTGCGA
    AGGATTCCCA AAAAGAACAA CCAGACAAAC GGTCAGGTGC TCAATGAGAG CCTGATCGCC
    TCGCTCCAGC ATTCACTGCA GCTAAGCCTC CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG
    AGCCCCTGCA GTGACTTTGA GGAACCCTGG CTCCCCAGGT CCAGAAGCCA GAGCATTGTT
    AGCGAGGGTA GTAGCTTGAC CTCCACCACC ACTCCCAACG TGTATTCTCT GTGTGGGGTC
    ACTCCGTCCC AGAGCGACAC AAGTAGCGTC AAGTCGGAAT ACACGGACCC TTGGGGTTAC
    TACATTGACT ACACGAGCTT GCAGGAAGAT CCAGGGAACC CCACCGGGGG CTGCTCAGCC
    AACACTGAGG CAGCAACTGG AAACGGGCCA GTACGCCACA TCCAGGAGGG GTCGAGAGTC
    CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT AGACCAAAGA TCGCCTCACC AGAGAAGTCA
    CAGAGAGTCA CCTCTCCATC CAGTGGGTAT TCTAGCCAGT CGAATACACC CACAGCACTC
    ACCCCTGTGC CTGTGTTTTT AAAATCAATG TCACCAGCAA ATGGAAAGGG GAAGGCCAAG
    CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG ATCTCTTCTA TGTCCATCTC ATCATCGTCC
    ACTTCTCTCT CTTCTAATAC TTCTACCGAA GGAAGTGGGA CCATGAAGAA ACTGGATACA
    ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT
    CTGGCAGACA AGTCCCCTTT TCTTCCACCA CCTCCTCCTC TAGCAGATTG CTCCGAGGGC
    TCTCCTCTGC CGCCCTCTCC CATGTTCCCC CCTCCACCAC CAGAAGCCCT TGTTCCCTTC
    TGCTCCCCCA CCGACGGGTG CCTTTCTCCT TCTCCCACAG CAGTTAGCCC CTCTCTTCCA
    AGATCCTTGC CTCCTGTGCC AGCACCTCCA CCTTTCTTGC CCTCATCAGA ACCCCCGCCC
    GCTCCGCCTC TGGACCCCAA ACTCATGAAA GAAAACAGGC CCTTTTTCAA AAACTCTAGC
    CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA
    CCCCCCATGC CCCTGATCAC CACAGAAGCT TTGCAGATGG TGCAGTTGAG GCCAGTGAGG
    AAGAACTCAG GTGCCGGGGC AGTCCTGTTT TCTGAACCAT CAGCTCAGGA ACAACGAACT
    CCGACTGCTC CACAGTATCA CTTAAAGCCA TCTGCTTTCC TCAAATCCCG AAATAGCATA
    AATGAAATGG AGAGTGAAAG CCAGGCTGCC TCTGTGACGA GCTCGCTTCC GATGCCTGCC
    AAGAGCCAGA GTCAGGGTGA CCATGACAGT GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC
    AGCGATGGAG CTCCAGGCCC GGGTCCCAGC CTCAGGACCA CTCTGCTCCC AGACTCTTCA
    CCCAGCAGGA AGCCACCCCC CATCTCTAAG AAGCCCAAAC TGTTCCTGGT GGTACCACCT
    CCGCAGAGAG ATTTCACAGC GGAGCCCACA GAGAACGGGA GCGAAGCTTT CCCAGGCGTG
    CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA
    GCATCCCGAG CTGGCTCTCA TGCTACGGCC CCCACCCCCG GCAGTCCGGC TCTAGAACCG
    GGAACTGCAG GGTCCTTATC CTCTAGCATT GTGGAAGCCA ATGTCCCCAT GGTCCAACCT
    AATACATCAC CGGGCCCCAC ACAGGAAGAG TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA
    AATGCGAAGA GCTGCCTGTC TCAACAGGGC AGAGAAGCTG GCTTGCTGGA ACCCAACACA
    GCTGCTTCCT CTTCAGACCC CGTGGATGTA TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG
    ACTCCGACTA AACCCAGGAC AACAGAAGAC CTGTTTGCAG CCATTCACAG ATCTAAAAGG
    AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC GATCACACTC GAAATCACTC TCCCTCCCCG
    CCTGTGACAC CCACGAGTGC TGCCCCTAAC CTGGCCTCTC CAAAGCAAGT AGGGTCTATC
    CAGAGAAGCA TAAAAAAGAG CACTACCAGC AGTGACAACT TCAAAGCTCT GCTGCTAAAG
    AAGGGGAGTC GCTCGGACAC CAGCGCTCGC ATGTCAGCGG CTGAGATGCT CAAGTCTACG
    GACCCTCGCT TCCAGAGATC TAGGTCGGAG CCTTCGGCAG ACAGCCCGGA CAGCCCATCC
    AGCTGCTCCC CCAACAAGAA CAGGAGAGCT CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG
    ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT CGGTACAGCC GCAGCAGGAC GCCACCCTCT
    GCGGCCAGCA GCAGGTACAG CATGCGGAAC CGCATCCAGA GCAGCCCCAT GACCGTCATC
    TCCGAGGGAG AGGGAGAACC TGCTGAGCCA GCGGACAACA AAGCACGCAG GGCCCTGGAT
    GCTACCAGGG TGTGCTCTCT GGACAGACTG ACGGGTCAGG AGATGGACCA GGCCAGCCTC
    CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT GGGATAGGAA GGGCTGAGGG CAACGGTCCC
    TCGGAGCAGT GTGGGGGTAC GGAACAGAAG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512750.1
    CDS856..5751
    Translation

    Target ORF information:

    RefSeq Version XM_006512687.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512687.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
    CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC
    AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA
    TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC
    CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC
    TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT
    GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT
    GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG
    TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA
    ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG
    CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT
    ATAAACATAA CTGGGGAGAA TTTCGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC
    ACAGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACAAT TTCAGGAATC
    CCTGACATCA TTCAGAAGGA GCTAGCATCA GCCACTGGCC AAGATGATGA TGGTAGTGCC
    CATTCGCTGT ACGTCCCAGA CCACTACTCT ACGTTAGGAA GGCTTGATAG TTATCGGTCC
    ACCGGGCAAT GCTTGGAAAC CAGGGACACC AGCTGTCAGA CCGAAGACGT GAAGGTCATC
    CCACCATCAA TGAGAAGGAT CAGGGCTCAC AAGGGGGTAG GCGTTGCTGC CCAGATGAGC
    CACCTCTCAG GTTCCTCAGG CAACATGTCT GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC
    CCATCTCGCC TCAGTAACGA TACTGGTTTC CACAGTCTTC CACGTACCGG CCCGAGAGCA
    AGCACCTATT CACTGGAGGG AAGGATGGGT GCCCTGGGCT CTACTGAAGA TACGGATGAC
    ACTTCTCCCT ACCAGGGGGG TAGCTTACAG GGGCATGAAA ACTTTGCGCA TTTAGGAGGT
    GCGTCGAGTA CTGGGATGCT TTCGAGGCCC AAATCCCAGC AGTTGAGGTT TTTGGAGAGT
    CCAGCATGTG TGGTTTCACC TCATGCAGCC TACTCTACCA GTGTCATCCC AAATGCCACG
    CTACTGTCCT CTTCAGAGGT TATTGTCATT CACACTGCTC AGAGTGCAGG ACAGCTGGAC
    AGTAGAACAC CCGGTTCATC TTCATATTCA AAAATAAAAC CCAGAGACCG TCCCACACCC
    AGATGTTCTG TGAAAGATGA CCATCAGTCC CCCCGTCATC ACTGGAACGA GGGTCATCTC
    ATTCATTCAC GGGCTTTAGC CTCCTCTGTC CCTGGTGCCA CCACGCTGTT ATCCCTCCAT
    GATTCAGAGG TCTCTCTAAA TGCCCCAGCA AACAGGGAAA ATGGATCCCA AGCCATACTC
    TATCATTGTA GAAACAACCC AAGCTTTCCT GACCACCCTT CAGATGTGGA TGGCAAGAGC
    GAGTGTAGTT ATTCGGGGGA CAGGGGATGT GGCAGTTCTG AGCCCTGGGA ATATAAAACC
    TCCAGCAATG GGCGGGCATC CCCACTGAAG CCACACCTGG CCACTCCTGG TTGCTCTACT
    CCCACAAGTA ACGTGAGCAG CTGCAGTTTG GACCAAACAT CTCTCAAGGG TGATACCAGG
    TCCCTGTGTT CAGAGGACCA TGATGGTTAC TATACTACCA CTCACGAGGC TGGGAACCTG
    TACACTCTCA GTGATGGCTT GGGGAACCCC AGGCACAGCA TGGTCAATGT TTTTGATGGA
    AGGGCTCAGA GAAGCCAAGG GGATCAGGCC GCTCACCAGG ATAAAATCCT TTCGAGAAAC
    ATCTCTCTGA AGAAAGCAAA GAAACCACCC CTGCCACCAT CCCGGACCGA TTCTCTGCGA
    AGGATTCCCA AAAAGAACAA CCAGACAAAC GGTCAGGTGC TCAATGAGAG CCTGATCGCC
    TCGCTCCAGC ATTCACTGCA GCTAAGCCTC CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG
    AGCCCCTGCA GTGACTTTGA GGAACCCTGG CTCCCCAGGT CCAGAAGCCA GAGCATTGTT
    AGCGAGGGTA GTAGCTTGAC CTCCACCACC ACTCCCAACG TGTATTCTCT GTGTGGGGTC
    ACTCCGTCCC AGAGCGACAC AAGTAGCGTC AAGTCGGAAT ACACGGACCC TTGGGGTTAC
    TACATTGACT ACACGAGCTT GCAGGAAGAT CCAGGGAACC CCACCGGGGG CTGCTCAGCC
    AACACTGAGG CAGCAACTGG AAACGGGCCA GTACGCCACA TCCAGGAGGG GTCGAGAGTC
    CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT AGACCAAAGA TCGCCTCACC AGAGAAGTCA
    CAGAGAGTCA CCTCTCCATC CAGTGGGTAT TCTAGCCAGT CGAATACACC CACAGCACTC
    ACCCCTGTGC CTGTGTTTTT AAAATCAATG TCACCAGCAA ATGGAAAGGG GAAGGCCAAG
    CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG ATCTCTTCTA TGTCCATCTC ATCATCGTCC
    ACTTCTCTCT CTTCTAATAC TTCTACCGAA GGAAGTGGGA CCATGAAGAA ACTGGATACA
    ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT
    CTGGCAGACA AGTCCCCTTT TCTTCCACCA CCTCCTCCTC TAGCAGATTG CTCCGAGGGC
    TCTCCTCTGC CGCCCTCTCC CATGTTCCCC CCTCCACCAC CAGAAGCCCT TGTTCCCTTC
    TGCTCCCCCA CCGACGGGTG CCTTTCTCCT TCTCCCACAG CAGTTAGCCC CTCTCTTCCA
    AGATCCTTGC CTCCTGTGCC AGCACCTCCA CCTTTCTTGC CCTCATCAGA ACCCCCGCCC
    GCTCCGCCTC TGGACCCCAA ACTCATGAAA GAAAACAGGC CCTTTTTCAA AAACTCTAGC
    CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA
    CCCCCCATGC CCCTGATCAC CACAGAAGCT TTGCAGATGG TGCAGTTGAG GCCAGTGAGG
    AAGAACTCAG GTGCCGGGGC AGTCCTGTTT TCTGAACCAT CAGCTCAGGA ACAACGAACT
    CCGACTGCTC CACAGTATCA CTTAAAGCCA TCTGCTTTCC TCAAATCCCG AAATAGCATA
    AATGAAATGG AGAGTGAAAG CCAGGCTGCC TCTGTGACGA GCTCGCTTCC GATGCCTGCC
    AAGAGCCAGA GTCAGGGTGA CCATGACAGT GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC
    AGCGATGGAG CTCCAGGCCC GGGTCCCAGC CTCAGGACCA CTCTGCTCCC AGACTCTTCA
    CCCAGCAGGA AGCCACCCCC CATCTCTAAG AAGCCCAAAC TGTTCCTGGT GGTACCACCT
    CCGCAGAGAG ATTTCACAGC GGAGCCCACA GAGAACGGGA GCGAAGCTTT CCCAGGCGTG
    CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA
    GCATCCCGAG CTGGCTCTCA TGCTACGGCC CCCACCCCCG GCAGTCCGGC TCTAGAACCG
    GGAACTGCAG GGTCCTTATC CTCTAGCATT GTGGAAGCCA ATGTCCCCAT GGTCCAACCT
    AATACATCAC CGGGCCCCAC ACAGGAAGAG TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA
    AATGCGAAGA GCTGCCTGTC TCAACAGGGC AGAGAAGCTG GCTTGCTGGA ACCCAACACA
    GCTGCTTCCT CTTCAGACCC CGTGGATGTA TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG
    ACTCCGACTA AACCCAGGAC AACAGAAGAC CTGTTTGCAG CCATTCACAG ATCTAAAAGG
    AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC GATCACACTC GAAATCACTC TCCCTCCCCG
    CCTGTGACAC CCACGAGTGC TGCCCCTAAC CTGGCCTCTC CAAAGCAAGT AGGGTCTATC
    CAGAGAAGCA TAAAAAAGAG CACTACCAGC AGTGACAACT TCAAAGCTCT GCTGCTAAAG
    AAGGGGAGTC GCTCGGACAC CAGCGCTCGC ATGTCAGCGG CTGAGATGCT CAAGTCTACG
    GACCCTCGCT TCCAGAGATC TAGGTCGGAG CCTTCGGCAG ACAGCCCGGA CAGCCCATCC
    AGCTGCTCCC CCAACAAGAA CAGGAGAGCT CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG
    ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT CGGTACAGCC GCAGCAGGAC GCCACCCTCT
    GCGGCCAGCA GCAGGTACAG CATGCGGAAC CGCATCCAGA GCAGCCCCAT GACCGTCATC
    TCCGAGGGAG AGGGAGAACC TGCTGAGCCA GCGGACAACA AAGCACGCAG GGCCCTGGAT
    GCTACCAGGG TGTGCTCTCT GGACAGACTG ACGGGTCAGG AGATGGACCA GGCCAGCCTC
    CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT GGGATAGGAA GGGCTGAGGG CAACGGTCCC
    TCGGAGCAGT GTGGGGGTAC GGAACAGAAG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43845
    Clone ID Related Accession (Same CDS sequence) XM_006512688.3 , XM_006512688.4
    Accession Version XM_006512688.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
    CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC
    CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG
    GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG
    GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC
    AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG
    AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG
    CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT
    TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT
    CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA
    TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT
    CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA
    GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGCATCAGC CACTGGCCAA
    GATGATGATG GTAGTGCCCA TTCGCTGTAC GTCCCAGACC ACTACTCTAC GTTAGGAAGG
    CTTGATAGTT ATCGGTCCAC CGGGCAATGC TTGGAAACCA GGGACACCAG CTGTCAGACC
    GAAGACGTGA AGGTCATCCC ACCATCAATG AGAAGGATCA GGGCTCACAA GGGGGTAGGC
    GTTGCTGCCC AGATGAGCCA CCTCTCAGGT TCCTCAGGCA ACATGTCTGT GCTGAGTGAC
    TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC AGTAACGATA CTGGTTTCCA CAGTCTTCCA
    CGTACCGGCC CGAGAGCAAG CACCTATTCA CTGGAGGGAA GGATGGGTGC CCTGGGCTCT
    ACTGAAGATA CGGATGACAC TTCTCCCTAC CAGGGGGGTA GCTTACAGGG GCATGAAAAC
    TTTGCGCATT TAGGAGGTGC GTCGAGTACT GGGATGCTTT CGAGGCCCAA ATCCCAGCAG
    TTGAGGTTTT TGGAGAGTCC AGCATGTGTG GTTTCACCTC ATGCAGCCTA CTCTACCAGT
    GTCATCCCAA ATGCCACGCT ACTGTCCTCT TCAGAGGTTA TTGTCATTCA CACTGCTCAG
    AGTGCAGGAC AGCTGGACAG TAGAACACCC GGTTCATCTT CATATTCAAA AATAAAACCC
    AGAGACCGTC CCACACCCAG ATGTTCTGTG AAAGATGACC ATCAGTCCCC CCGTCATCAC
    TGGAACGAGG GTCATCTCAT TCATTCACGG GCTTTAGCCT CCTCTGTCCC TGGTGCCACC
    ACGCTGTTAT CCCTCCATGA TTCAGAGGTC TCTCTAAATG CCCCAGCAAA CAGGGAAAAT
    GGATCCCAAG CCATACTCTA TCATTGTAGA AACAACCCAA GCTTTCCTGA CCACCCTTCA
    GATGTGGATG GCAAGAGCGA GTGTAGTTAT TCGGGGGACA GGGGATGTGG CAGTTCTGAG
    CCCTGGGAAT ATAAAACCTC CAGCAATGGG CGGGCATCCC CACTGAAGCC ACACCTGGCC
    ACTCCTGGTT GCTCTACTCC CACAAGTAAC GTGAGCAGCT GCAGTTTGGA CCAAACATCT
    CTCAAGGGTG ATACCAGGTC CCTGTGTTCA GAGGACCATG ATGGTTACTA TACTACCACT
    CACGAGGCTG GGAACCTGTA CACTCTCAGT GATGGCTTGG GGAACCCCAG GCACAGCATG
    GTCAATGTTT TTGATGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCGC TCACCAGGAT
    AAAATCCTTT CGAGAAACAT CTCTCTGAAG AAAGCAAAGA AACCACCCCT GCCACCATCC
    CGGACCGATT CTCTGCGAAG GATTCCCAAA AAGAACAACC AGACAAACGG TCAGGTGCTC
    AATGAGAGCC TGATCGCCTC GCTCCAGCAT TCACTGCAGC TAAGCCTCCC TGGCAAGGGC
    GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTTTGAGG AACCCTGGCT CCCCAGGTCC
    AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG
    TATTCTCTGT GTGGGGTCAC TCCGTCCCAG AGCGACACAA GTAGCGTCAA GTCGGAATAC
    ACGGACCCTT GGGGTTACTA CATTGACTAC ACGAGCTTGC AGGAAGATCC AGGGAACCCC
    ACCGGGGGCT GCTCAGCCAA CACTGAGGCA GCAACTGGAA ACGGGCCAGT ACGCCACATC
    CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA GTGCCGGGCT GCTCAGTTAG ACCAAAGATC
    GCCTCACCAG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG
    AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAATGTC ACCAGCAAAT
    GGAAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCTATG
    TCCATCTCAT CATCGTCCAC TTCTCTCTCT TCTAATACTT CTACCGAAGG AAGTGGGACC
    ATGAAGAAAC TGGATACAAC CCTGGCCTCG GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC
    CCTCTGCCTT CTCCGTGTCT GGCAGACAAG TCCCCTTTTC TTCCACCACC TCCTCCTCTA
    GCAGATTGCT CCGAGGGCTC TCCTCTGCCG CCCTCTCCCA TGTTCCCCCC TCCACCACCA
    GAAGCCCTTG TTCCCTTCTG CTCCCCCACC GACGGGTGCC TTTCTCCTTC TCCCACAGCA
    GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT CCTGTGCCAG CACCTCCACC TTTCTTGCCC
    TCATCAGAAC CCCCGCCCGC TCCGCCTCTG GACCCCAAAC TCATGAAAGA AAACAGGCCC
    TTTTTCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC
    AAGGAGGAGG GCTGCCGACC CCCCATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG
    CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCCGGGGCAG TCCTGTTTTC TGAACCATCA
    GCTCAGGAAC AACGAACTCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC
    AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC
    TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTAGAGCGT
    GGCGGCCTTC CGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCACT
    CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCTAAGAA GCCCAAACTG
    TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC
    GAAGCTTTCC CAGGCGTGCC CAGCCCTACC AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA
    GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT GGCTCTCATG CTACGGCCCC CACCCCCGGC
    AGTCCGGCTC TAGAACCGGG AACTGCAGGG TCCTTATCCT CTAGCATTGT GGAAGCCAAT
    GTCCCCATGG TCCAACCTAA TACATCACCG GGCCCCACAC AGGAAGAGTC AGGCGAGAAC
    AGTGTGGATG GTGAGAGAAA TGCGAAGAGC TGCCTGTCTC AACAGGGCAG AGAAGCTGGC
    TTGCTGGAAC CCAACACAGC TGCTTCCTCT TCAGACCCCG TGGATGTATC TAAGGAAGAG
    GGAAGCGATG AGGTGCTGAC TCCGACTAAA CCCAGGACAA CAGAAGACCT GTTTGCAGCC
    ATTCACAGAT CTAAAAGGAA AGTCCTTGGC CGGAAAGACT CAGAAGACGA TCACACTCGA
    AATCACTCTC CCTCCCCGCC TGTGACACCC ACGAGTGCTG CCCCTAACCT GGCCTCTCCA
    AAGCAAGTAG GGTCTATCCA GAGAAGCATA AAAAAGAGCA CTACCAGCAG TGACAACTTC
    AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCAGCGGCT
    GAGATGCTCA AGTCTACGGA CCCTCGCTTC CAGAGATCTA GGTCGGAGCC TTCGGCAGAC
    AGCCCGGACA GCCCATCCAG CTGCTCCCCC AACAAGAACA GGAGAGCTCA GGAAGAGTGG
    GCCAAGAACG AAGGCCTGAT GCCTCGGAGT CTGTCCTTTT CGGGCCCTCG GTACAGCCGC
    AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGCGGAACCG CATCCAGAGC
    AGCCCCATGA CCGTCATCTC CGAGGGAGAG GGAGAACCTG CTGAGCCAGC GGACAACAAA
    GCACGCAGGG CCCTGGATGC TACCAGGGTG TGCTCTCTGG ACAGACTGAC GGGTCAGGAG
    ATGGACCAGG CCAGCCTCCT CTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG
    GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT GGGGGTACGG AACAGAAGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512751.1
    CDS422..5275
    Translation

    Target ORF information:

    RefSeq Version XM_006512688.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512688.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
    CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC
    CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG
    GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG
    GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC
    AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG
    AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG
    CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT
    TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT
    CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA
    TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT
    CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA
    GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGCATCAGC CACTGGCCAA
    GATGATGATG GTAGTGCCCA TTCGCTGTAC GTCCCAGACC ACTACTCTAC GTTAGGAAGG
    CTTGATAGTT ATCGGTCCAC CGGGCAATGC TTGGAAACCA GGGACACCAG CTGTCAGACC
    GAAGACGTGA AGGTCATCCC ACCATCAATG AGAAGGATCA GGGCTCACAA GGGGGTAGGC
    GTTGCTGCCC AGATGAGCCA CCTCTCAGGT TCCTCAGGCA ACATGTCTGT GCTGAGTGAC
    TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC AGTAACGATA CTGGTTTCCA CAGTCTTCCA
    CGTACCGGCC CGAGAGCAAG CACCTATTCA CTGGAGGGAA GGATGGGTGC CCTGGGCTCT
    ACTGAAGATA CGGATGACAC TTCTCCCTAC CAGGGGGGTA GCTTACAGGG GCATGAAAAC
    TTTGCGCATT TAGGAGGTGC GTCGAGTACT GGGATGCTTT CGAGGCCCAA ATCCCAGCAG
    TTGAGGTTTT TGGAGAGTCC AGCATGTGTG GTTTCACCTC ATGCAGCCTA CTCTACCAGT
    GTCATCCCAA ATGCCACGCT ACTGTCCTCT TCAGAGGTTA TTGTCATTCA CACTGCTCAG
    AGTGCAGGAC AGCTGGACAG TAGAACACCC GGTTCATCTT CATATTCAAA AATAAAACCC
    AGAGACCGTC CCACACCCAG ATGTTCTGTG AAAGATGACC ATCAGTCCCC CCGTCATCAC
    TGGAACGAGG GTCATCTCAT TCATTCACGG GCTTTAGCCT CCTCTGTCCC TGGTGCCACC
    ACGCTGTTAT CCCTCCATGA TTCAGAGGTC TCTCTAAATG CCCCAGCAAA CAGGGAAAAT
    GGATCCCAAG CCATACTCTA TCATTGTAGA AACAACCCAA GCTTTCCTGA CCACCCTTCA
    GATGTGGATG GCAAGAGCGA GTGTAGTTAT TCGGGGGACA GGGGATGTGG CAGTTCTGAG
    CCCTGGGAAT ATAAAACCTC CAGCAATGGG CGGGCATCCC CACTGAAGCC ACACCTGGCC
    ACTCCTGGTT GCTCTACTCC CACAAGTAAC GTGAGCAGCT GCAGTTTGGA CCAAACATCT
    CTCAAGGGTG ATACCAGGTC CCTGTGTTCA GAGGACCATG ATGGTTACTA TACTACCACT
    CACGAGGCTG GGAACCTGTA CACTCTCAGT GATGGCTTGG GGAACCCCAG GCACAGCATG
    GTCAATGTTT TTGATGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCGC TCACCAGGAT
    AAAATCCTTT CGAGAAACAT CTCTCTGAAG AAAGCAAAGA AACCACCCCT GCCACCATCC
    CGGACCGATT CTCTGCGAAG GATTCCCAAA AAGAACAACC AGACAAACGG TCAGGTGCTC
    AATGAGAGCC TGATCGCCTC GCTCCAGCAT TCACTGCAGC TAAGCCTCCC TGGCAAGGGC
    GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTTTGAGG AACCCTGGCT CCCCAGGTCC
    AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG
    TATTCTCTGT GTGGGGTCAC TCCGTCCCAG AGCGACACAA GTAGCGTCAA GTCGGAATAC
    ACGGACCCTT GGGGTTACTA CATTGACTAC ACGAGCTTGC AGGAAGATCC AGGGAACCCC
    ACCGGGGGCT GCTCAGCCAA CACTGAGGCA GCAACTGGAA ACGGGCCAGT ACGCCACATC
    CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA GTGCCGGGCT GCTCAGTTAG ACCAAAGATC
    GCCTCACCAG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG
    AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAATGTC ACCAGCAAAT
    GGAAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCTATG
    TCCATCTCAT CATCGTCCAC TTCTCTCTCT TCTAATACTT CTACCGAAGG AAGTGGGACC
    ATGAAGAAAC TGGATACAAC CCTGGCCTCG GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC
    CCTCTGCCTT CTCCGTGTCT GGCAGACAAG TCCCCTTTTC TTCCACCACC TCCTCCTCTA
    GCAGATTGCT CCGAGGGCTC TCCTCTGCCG CCCTCTCCCA TGTTCCCCCC TCCACCACCA
    GAAGCCCTTG TTCCCTTCTG CTCCCCCACC GACGGGTGCC TTTCTCCTTC TCCCACAGCA
    GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT CCTGTGCCAG CACCTCCACC TTTCTTGCCC
    TCATCAGAAC CCCCGCCCGC TCCGCCTCTG GACCCCAAAC TCATGAAAGA AAACAGGCCC
    TTTTTCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC
    AAGGAGGAGG GCTGCCGACC CCCCATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG
    CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCCGGGGCAG TCCTGTTTTC TGAACCATCA
    GCTCAGGAAC AACGAACTCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC
    AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC
    TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTAGAGCGT
    GGCGGCCTTC CGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCACT
    CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCTAAGAA GCCCAAACTG
    TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC
    GAAGCTTTCC CAGGCGTGCC CAGCCCTACC AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA
    GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT GGCTCTCATG CTACGGCCCC CACCCCCGGC
    AGTCCGGCTC TAGAACCGGG AACTGCAGGG TCCTTATCCT CTAGCATTGT GGAAGCCAAT
    GTCCCCATGG TCCAACCTAA TACATCACCG GGCCCCACAC AGGAAGAGTC AGGCGAGAAC
    AGTGTGGATG GTGAGAGAAA TGCGAAGAGC TGCCTGTCTC AACAGGGCAG AGAAGCTGGC
    TTGCTGGAAC CCAACACAGC TGCTTCCTCT TCAGACCCCG TGGATGTATC TAAGGAAGAG
    GGAAGCGATG AGGTGCTGAC TCCGACTAAA CCCAGGACAA CAGAAGACCT GTTTGCAGCC
    ATTCACAGAT CTAAAAGGAA AGTCCTTGGC CGGAAAGACT CAGAAGACGA TCACACTCGA
    AATCACTCTC CCTCCCCGCC TGTGACACCC ACGAGTGCTG CCCCTAACCT GGCCTCTCCA
    AAGCAAGTAG GGTCTATCCA GAGAAGCATA AAAAAGAGCA CTACCAGCAG TGACAACTTC
    AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCAGCGGCT
    GAGATGCTCA AGTCTACGGA CCCTCGCTTC CAGAGATCTA GGTCGGAGCC TTCGGCAGAC
    AGCCCGGACA GCCCATCCAG CTGCTCCCCC AACAAGAACA GGAGAGCTCA GGAAGAGTGG
    GCCAAGAACG AAGGCCTGAT GCCTCGGAGT CTGTCCTTTT CGGGCCCTCG GTACAGCCGC
    AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGCGGAACCG CATCCAGAGC
    AGCCCCATGA CCGTCATCTC CGAGGGAGAG GGAGAACCTG CTGAGCCAGC GGACAACAAA
    GCACGCAGGG CCCTGGATGC TACCAGGGTG TGCTCTCTGG ACAGACTGAC GGGTCAGGAG
    ATGGACCAGG CCAGCCTCCT CTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG
    GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT GGGGGTACGG AACAGAAGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43846
    Clone ID Related Accession (Same CDS sequence) XM_006512689.3 , XM_006512689.4
    Accession Version XM_006512689.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
    CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG
    CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC
    CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT
    GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC
    TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT
    GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC
    GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG
    ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGGGGAG
    AATTTCGATC GTCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA
    CGACCGAAGA AAGTCAAAAG GAGAAAGACA ATTTCAGGAA TCCCTGACAT CATTCAGAAG
    GAGCTAGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA
    GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA
    ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG
    ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA
    GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC
    GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG
    GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG
    GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG
    CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA
    CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG
    GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA
    TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT
    GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA
    GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA
    AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC
    CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG
    GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA
    TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC
    AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC
    CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC
    TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA
    GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA
    AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC
    AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG
    CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT
    GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG
    ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC
    ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC
    TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT
    GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG
    GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA
    TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT
    TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA
    AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT
    ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT
    GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT
    TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT
    CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG
    TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG
    CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC
    AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG
    GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC
    ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG
    GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT
    CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT
    GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC
    CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC
    CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA
    GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA
    GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT
    CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA
    TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC
    ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG
    TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC
    CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG
    ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA
    GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT
    GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG
    AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC
    ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA
    TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG
    AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA
    CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT
    CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT
    GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT
    ACGGAACAGA AGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512752.1
    CDS321..5078
    Translation

    Target ORF information:

    RefSeq Version XM_006512689.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512689.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
    CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG
    CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC
    CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT
    GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC
    TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT
    GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC
    GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG
    ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGGGGAG
    AATTTCGATC GTCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA
    CGACCGAAGA AAGTCAAAAG GAGAAAGACA ATTTCAGGAA TCCCTGACAT CATTCAGAAG
    GAGCTAGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA
    GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA
    ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG
    ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA
    GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC
    GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG
    GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG
    GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG
    CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA
    CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG
    GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA
    TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT
    GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA
    GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA
    AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC
    CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG
    GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA
    TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC
    AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC
    CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC
    TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA
    GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA
    AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC
    AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG
    CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT
    GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG
    ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC
    ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC
    TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT
    GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG
    GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA
    TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT
    TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA
    AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT
    ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT
    GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT
    TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT
    CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG
    TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG
    CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC
    AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG
    GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC
    ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG
    GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT
    CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA
    AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT
    GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC
    CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC
    CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA
    GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA
    GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT
    CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA
    TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC
    ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG
    TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC
    CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG
    ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA
    GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT
    GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG
    AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC
    ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA
    TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG
    AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC
    TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC
    AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA
    CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT
    CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT
    GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT
    ACGGAACAGA AGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43847
    Clone ID Related Accession (Same CDS sequence) XM_006512690.4 , XM_006512690.3
    Accession Version XM_006512690.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
    ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG
    CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC
    GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT
    CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA
    GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA
    CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC
    ATAACTGGGG AGAATTTCGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC
    ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CAATTTCAGG AATCCCTGAC
    ATCATTCAGA AGGAGCTAGC ATCAGCCACT GGCCAAGATG ATGATGGTAG TGCCCATTCG
    CTGTACGTCC CAGACCACTA CTCTACGTTA GGAAGGCTTG ATAGTTATCG GTCCACCGGG
    CAATGCTTGG AAACCAGGGA CACCAGCTGT CAGACCGAAG ACGTGAAGGT CATCCCACCA
    TCAATGAGAA GGATCAGGGC TCACAAGGGG GTAGGCGTTG CTGCCCAGAT GAGCCACCTC
    TCAGGTTCCT CAGGCAACAT GTCTGTGCTG AGTGACTCAG CCGGTGTTGT GTTCCCATCT
    CGCCTCAGTA ACGATACTGG TTTCCACAGT CTTCCACGTA CCGGCCCGAG AGCAAGCACC
    TATTCACTGG AGGGAAGGAT GGGTGCCCTG GGCTCTACTG AAGATACGGA TGACACTTCT
    CCCTACCAGG GGGGTAGCTT ACAGGGGCAT GAAAACTTTG CGCATTTAGG AGGTGCGTCG
    AGTACTGGGA TGCTTTCGAG GCCCAAATCC CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA
    TGTGTGGTTT CACCTCATGC AGCCTACTCT ACCAGTGTCA TCCCAAATGC CACGCTACTG
    TCCTCTTCAG AGGTTATTGT CATTCACACT GCTCAGAGTG CAGGACAGCT GGACAGTAGA
    ACACCCGGTT CATCTTCATA TTCAAAAATA AAACCCAGAG ACCGTCCCAC ACCCAGATGT
    TCTGTGAAAG ATGACCATCA GTCCCCCCGT CATCACTGGA ACGAGGGTCA TCTCATTCAT
    TCACGGGCTT TAGCCTCCTC TGTCCCTGGT GCCACCACGC TGTTATCCCT CCATGATTCA
    GAGGTCTCTC TAAATGCCCC AGCAAACAGG GAAAATGGAT CCCAAGCCAT ACTCTATCAT
    TGTAGAAACA ACCCAAGCTT TCCTGACCAC CCTTCAGATG TGGATGGCAA GAGCGAGTGT
    AGTTATTCGG GGGACAGGGG ATGTGGCAGT TCTGAGCCCT GGGAATATAA AACCTCCAGC
    AATGGGCGGG CATCCCCACT GAAGCCACAC CTGGCCACTC CTGGTTGCTC TACTCCCACA
    AGTAACGTGA GCAGCTGCAG TTTGGACCAA ACATCTCTCA AGGGTGATAC CAGGTCCCTG
    TGTTCAGAGG ACCATGATGG TTACTATACT ACCACTCACG AGGCTGGGAA CCTGTACACT
    CTCAGTGATG GCTTGGGGAA CCCCAGGCAC AGCATGGTCA ATGTTTTTGA TGGAAGGGCT
    CAGAGAAGCC AAGGGGATCA GGCCGCTCAC CAGGATAAAA TCCTTTCGAG AAACATCTCT
    CTGAAGAAAG CAAAGAAACC ACCCCTGCCA CCATCCCGGA CCGATTCTCT GCGAAGGATT
    CCCAAAAAGA ACAACCAGAC AAACGGTCAG GTGCTCAATG AGAGCCTGAT CGCCTCGCTC
    CAGCATTCAC TGCAGCTAAG CCTCCCTGGC AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC
    TGCAGTGACT TTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG
    GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGTGG GGTCACTCCG
    TCCCAGAGCG ACACAAGTAG CGTCAAGTCG GAATACACGG ACCCTTGGGG TTACTACATT
    GACTACACGA GCTTGCAGGA AGATCCAGGG AACCCCACCG GGGGCTGCTC AGCCAACACT
    GAGGCAGCAA CTGGAAACGG GCCAGTACGC CACATCCAGG AGGGGTCGAG AGTCCCAGTG
    CCCCAAGTGC CGGGCTGCTC AGTTAGACCA AAGATCGCCT CACCAGAGAA GTCACAGAGA
    GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCT
    GTGCCTGTGT TTTTAAAATC AATGTCACCA GCAAATGGAA AGGGGAAGGC CAAGCCCAAG
    GTACCAGAAA GAAAATCGTC TCTGATCTCT TCTATGTCCA TCTCATCATC GTCCACTTCT
    CTCTCTTCTA ATACTTCTAC CGAAGGAAGT GGGACCATGA AGAAACTGGA TACAACCCTG
    GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA
    GACAAGTCCC CTTTTCTTCC ACCACCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT
    CTGCCGCCCT CTCCCATGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC
    CCCACCGACG GGTGCCTTTC TCCTTCTCCC ACAGCAGTTA GCCCCTCTCT TCCAAGATCC
    TTGCCTCCTG TGCCAGCACC TCCACCTTTC TTGCCCTCAT CAGAACCCCC GCCCGCTCCG
    CCTCTGGACC CCAAACTCAT GAAAGAAAAC AGGCCCTTTT TCAAAAACTC TAGCCAGTCC
    GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCCCC
    ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC
    TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA CCATCAGCTC AGGAACAACG AACTCCGACT
    GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA
    ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC
    CAGAGTCAGG GTGACCATGA CAGTGCAGTA GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT
    GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCACTCTGC TCCCAGACTC TTCACCCAGC
    AGGAAGCCAC CCCCCATCTC TAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG
    AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC
    CCTACCAGGG CAGAGGGGGA AGCTGTGCGG AGCCAAGAGG AGAAATCCAG CCCAGCATCC
    CGAGCTGGCT CTCATGCTAC GGCCCCCACC CCCGGCAGTC CGGCTCTAGA ACCGGGAACT
    GCAGGGTCCT TATCCTCTAG CATTGTGGAA GCCAATGTCC CCATGGTCCA ACCTAATACA
    TCACCGGGCC CCACACAGGA AGAGTCAGGC GAGAACAGTG TGGATGGTGA GAGAAATGCG
    AAGAGCTGCC TGTCTCAACA GGGCAGAGAA GCTGGCTTGC TGGAACCCAA CACAGCTGCT
    TCCTCTTCAG ACCCCGTGGA TGTATCTAAG GAAGAGGGAA GCGATGAGGT GCTGACTCCG
    ACTAAACCCA GGACAACAGA AGACCTGTTT GCAGCCATTC ACAGATCTAA AAGGAAAGTC
    CTTGGCCGGA AAGACTCAGA AGACGATCAC ACTCGAAATC ACTCTCCCTC CCCGCCTGTG
    ACACCCACGA GTGCTGCCCC TAACCTGGCC TCTCCAAAGC AAGTAGGGTC TATCCAGAGA
    AGCATAAAAA AGAGCACTAC CAGCAGTGAC AACTTCAAAG CTCTGCTGCT AAAGAAGGGG
    AGTCGCTCGG ACACCAGCGC TCGCATGTCA GCGGCTGAGA TGCTCAAGTC TACGGACCCT
    CGCTTCCAGA GATCTAGGTC GGAGCCTTCG GCAGACAGCC CGGACAGCCC ATCCAGCTGC
    TCCCCCAACA AGAACAGGAG AGCTCAGGAA GAGTGGGCCA AGAACGAAGG CCTGATGCCT
    CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC
    AGCAGCAGGT ACAGCATGCG GAACCGCATC CAGAGCAGCC CCATGACCGT CATCTCCGAG
    GGAGAGGGAG AACCTGCTGA GCCAGCGGAC AACAAAGCAC GCAGGGCCCT GGATGCTACC
    AGGGTGTGCT CTCTGGACAG ACTGACGGGT CAGGAGATGG ACCAGGCCAG CCTCCTCTGT
    AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTG AGGGCAACGG TCCCTCGGAG
    CAGTGTGGGG GTACGGAACA GAAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512753.1
    CDS2556..7265
    Translation

    Target ORF information:

    RefSeq Version XM_006512690.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512690.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
    ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT
    GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG
    CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC
    GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT
    CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA
    GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA
    CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC
    ATAACTGGGG AGAATTTCGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC
    ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CAATTTCAGG AATCCCTGAC
    ATCATTCAGA AGGAGCTAGC ATCAGCCACT GGCCAAGATG ATGATGGTAG TGCCCATTCG
    CTGTACGTCC CAGACCACTA CTCTACGTTA GGAAGGCTTG ATAGTTATCG GTCCACCGGG
    CAATGCTTGG AAACCAGGGA CACCAGCTGT CAGACCGAAG ACGTGAAGGT CATCCCACCA
    TCAATGAGAA GGATCAGGGC TCACAAGGGG GTAGGCGTTG CTGCCCAGAT GAGCCACCTC
    TCAGGTTCCT CAGGCAACAT GTCTGTGCTG AGTGACTCAG CCGGTGTTGT GTTCCCATCT
    CGCCTCAGTA ACGATACTGG TTTCCACAGT CTTCCACGTA CCGGCCCGAG AGCAAGCACC
    TATTCACTGG AGGGAAGGAT GGGTGCCCTG GGCTCTACTG AAGATACGGA TGACACTTCT
    CCCTACCAGG GGGGTAGCTT ACAGGGGCAT GAAAACTTTG CGCATTTAGG AGGTGCGTCG
    AGTACTGGGA TGCTTTCGAG GCCCAAATCC CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA
    TGTGTGGTTT CACCTCATGC AGCCTACTCT ACCAGTGTCA TCCCAAATGC CACGCTACTG
    TCCTCTTCAG AGGTTATTGT CATTCACACT GCTCAGAGTG CAGGACAGCT GGACAGTAGA
    ACACCCGGTT CATCTTCATA TTCAAAAATA AAACCCAGAG ACCGTCCCAC ACCCAGATGT
    TCTGTGAAAG ATGACCATCA GTCCCCCCGT CATCACTGGA ACGAGGGTCA TCTCATTCAT
    TCACGGGCTT TAGCCTCCTC TGTCCCTGGT GCCACCACGC TGTTATCCCT CCATGATTCA
    GAGGTCTCTC TAAATGCCCC AGCAAACAGG GAAAATGGAT CCCAAGCCAT ACTCTATCAT
    TGTAGAAACA ACCCAAGCTT TCCTGACCAC CCTTCAGATG TGGATGGCAA GAGCGAGTGT
    AGTTATTCGG GGGACAGGGG ATGTGGCAGT TCTGAGCCCT GGGAATATAA AACCTCCAGC
    AATGGGCGGG CATCCCCACT GAAGCCACAC CTGGCCACTC CTGGTTGCTC TACTCCCACA
    AGTAACGTGA GCAGCTGCAG TTTGGACCAA ACATCTCTCA AGGGTGATAC CAGGTCCCTG
    TGTTCAGAGG ACCATGATGG TTACTATACT ACCACTCACG AGGCTGGGAA CCTGTACACT
    CTCAGTGATG GCTTGGGGAA CCCCAGGCAC AGCATGGTCA ATGTTTTTGA TGGAAGGGCT
    CAGAGAAGCC AAGGGGATCA GGCCGCTCAC CAGGATAAAA TCCTTTCGAG AAACATCTCT
    CTGAAGAAAG CAAAGAAACC ACCCCTGCCA CCATCCCGGA CCGATTCTCT GCGAAGGATT
    CCCAAAAAGA ACAACCAGAC AAACGGTCAG GTGCTCAATG AGAGCCTGAT CGCCTCGCTC
    CAGCATTCAC TGCAGCTAAG CCTCCCTGGC AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC
    TGCAGTGACT TTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG
    GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGTGG GGTCACTCCG
    TCCCAGAGCG ACACAAGTAG CGTCAAGTCG GAATACACGG ACCCTTGGGG TTACTACATT
    GACTACACGA GCTTGCAGGA AGATCCAGGG AACCCCACCG GGGGCTGCTC AGCCAACACT
    GAGGCAGCAA CTGGAAACGG GCCAGTACGC CACATCCAGG AGGGGTCGAG AGTCCCAGTG
    CCCCAAGTGC CGGGCTGCTC AGTTAGACCA AAGATCGCCT CACCAGAGAA GTCACAGAGA
    GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCT
    GTGCCTGTGT TTTTAAAATC AATGTCACCA GCAAATGGAA AGGGGAAGGC CAAGCCCAAG
    GTACCAGAAA GAAAATCGTC TCTGATCTCT TCTATGTCCA TCTCATCATC GTCCACTTCT
    CTCTCTTCTA ATACTTCTAC CGAAGGAAGT GGGACCATGA AGAAACTGGA TACAACCCTG
    GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA
    GACAAGTCCC CTTTTCTTCC ACCACCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT
    CTGCCGCCCT CTCCCATGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC
    CCCACCGACG GGTGCCTTTC TCCTTCTCCC ACAGCAGTTA GCCCCTCTCT TCCAAGATCC
    TTGCCTCCTG TGCCAGCACC TCCACCTTTC TTGCCCTCAT CAGAACCCCC GCCCGCTCCG
    CCTCTGGACC CCAAACTCAT GAAAGAAAAC AGGCCCTTTT TCAAAAACTC TAGCCAGTCC
    GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCCCC
    ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC
    TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA CCATCAGCTC AGGAACAACG AACTCCGACT
    GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA
    ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC
    CAGAGTCAGG GTGACCATGA CAGTGCAGTA GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT
    GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCACTCTGC TCCCAGACTC TTCACCCAGC
    AGGAAGCCAC CCCCCATCTC TAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG
    AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC
    CCTACCAGGG CAGAGGGGGA AGCTGTGCGG AGCCAAGAGG AGAAATCCAG CCCAGCATCC
    CGAGCTGGCT CTCATGCTAC GGCCCCCACC CCCGGCAGTC CGGCTCTAGA ACCGGGAACT
    GCAGGGTCCT TATCCTCTAG CATTGTGGAA GCCAATGTCC CCATGGTCCA ACCTAATACA
    TCACCGGGCC CCACACAGGA AGAGTCAGGC GAGAACAGTG TGGATGGTGA GAGAAATGCG
    AAGAGCTGCC TGTCTCAACA GGGCAGAGAA GCTGGCTTGC TGGAACCCAA CACAGCTGCT
    TCCTCTTCAG ACCCCGTGGA TGTATCTAAG GAAGAGGGAA GCGATGAGGT GCTGACTCCG
    ACTAAACCCA GGACAACAGA AGACCTGTTT GCAGCCATTC ACAGATCTAA AAGGAAAGTC
    CTTGGCCGGA AAGACTCAGA AGACGATCAC ACTCGAAATC ACTCTCCCTC CCCGCCTGTG
    ACACCCACGA GTGCTGCCCC TAACCTGGCC TCTCCAAAGC AAGTAGGGTC TATCCAGAGA
    AGCATAAAAA AGAGCACTAC CAGCAGTGAC AACTTCAAAG CTCTGCTGCT AAAGAAGGGG
    AGTCGCTCGG ACACCAGCGC TCGCATGTCA GCGGCTGAGA TGCTCAAGTC TACGGACCCT
    CGCTTCCAGA GATCTAGGTC GGAGCCTTCG GCAGACAGCC CGGACAGCCC ATCCAGCTGC
    TCCCCCAACA AGAACAGGAG AGCTCAGGAA GAGTGGGCCA AGAACGAAGG CCTGATGCCT
    CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC
    AGCAGCAGGT ACAGCATGCG GAACCGCATC CAGAGCAGCC CCATGACCGT CATCTCCGAG
    GGAGAGGGAG AACCTGCTGA GCCAGCGGAC AACAAAGCAC GCAGGGCCCT GGATGCTACC
    AGGGTGTGCT CTCTGGACAG ACTGACGGGT CAGGAGATGG ACCAGGCCAG CCTCCTCTGT
    AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTG AGGGCAACGG TCCCTCGGAG
    CAGTGTGGGG GTACGGAACA GAAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.