Miga1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Miga1
Entrez Gene ID	215708
Full Name	mitoguardin 1
Synonyms	A930038D06,AI852168,C030011O14Rik,Fam73a,Mita1
General protein information	Preferred Names mitoguardin 1 Names mitoguardin 1 mitoguardin-1 family with sequence similarity 73, member A protein FAM73A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 H3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011240070.2	XP_011238372.1	mitoguardin-1 isoform X1
NM_001162375.1	NP_001155847.1	mitoguardin 1 isoform 2
XM_011240070.3	XP_011238372.1	mitoguardin 1 isoform X1
NM_174868.4	NP_777357.2	mitoguardin 1 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FAM73A	NP_001257313.1
Rattus norvegicus (Norway rat)	Fam73a	XP_006233576.1
Xenopus tropicalis (tropical clawed frog)	fam73a	NP_001120143.1
	LOC710882	XP_001099577.2
Canis lupus familiaris (dog)	FAM73A	XP_537107.4
Pan troglodytes (chimpanzee)	FAM73A	XP_524744.2
Gallus gallus (chicken)	FAM73A	XP_001234741.2
Danio rerio (zebrafish)	fam73a	NP_001006090.1
Bos taurus (cattle)	FAM73A	XP_002686344.1
Mus musculus (house mouse)	Fam73a	NP_777357.2

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial dynamics controls anti-tumour innate immunity by regulating CHIP-IRF1 axis stability.
Gao Z, Li Y, Wang F, Huang T, Fan K, Zhang Y, Zhong J, Cao Q, Chao T, Jia J, Yang S, Zhang L, Xiao Y, Zhou JY, Feng XH, Jin J
Nature communications8(1)1805(2017 Nov)

Mitochondrial Function Regulated by Mitoguardin-1/2 Is Crucial for Ovarian Endocrine Functions and Ovulation.
Liu XM, Zhang YL, Ji SY, Zhao LW, Shang WN, Li D, Chen Z, Tong C, Fan HY
Endocrinology158(11)3988-3999(2017 Nov)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.
Zhang Y, Liu X, Bai J, Tian X, Zhao X, Liu W, Duan X, Shang W, Fan HY, Tong C
Molecular cell61(1)111-24(2016 Jan)

Mitoguardin-1 and -2 promote maturation and the developmental potential of mouse oocytes by maintaining mitochondrial dynamics and functions.
Liu XM, Zhang YP, Ji SY, Li BT, Tian X, Li D, Tong C, Fan HY
Oncotarget7(2)1155-67(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The ovarian phenotypes of these mice suggested that MIGA1/2 proteins play an important role in ovulation and ovarian steroidogenesis. In vivo and in vitro analyses of Miga1/2-knockout granulosa cells showed severe defects in luteinization and steroidogenesis and disordered mitochondrial morphology and function in response to gonadotropins.
Title: Mitochondrial Function Regulated by Mitoguardin-1/2 Is Crucial for Ovarian Endocrine Functions and Ovulation.

Propose that MIGA1/2 proteins promote mitochondrial fusion by regulating mitochondrial phospholipid metabolism via MitoPLD.
Title: Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.

Data indicate that mitoguardin (Miga) knoskout Miga1/2-/- females show greatly reduced quality of oocytes and early embryos and are subfertile.
Title: Mitoguardin-1 and -2 promote maturation and the developmental potential of mouse oocytes by maintaining mitochondrial dynamics and functions.

The following Miga1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Miga1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04705	NM_174868.4 Latest version!	Mus musculus mitoguardin 1 (Miga1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu08363	NM_001162375.1 Latest version!	Mus musculus mitoguardin 1 (Miga1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu72934	XM_011240070.3 Latest version!	Mus musculus mitoguardin 1 (Miga1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu72934	XM_011240070.2	Mus musculus family with sequence similarity 73, member A (Fam73a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Miga1 ( NM_174868.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_777357.2 Mus musculus mitoguardin 1 (Miga1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu04705
Clone ID Related Accession (Same CDS sequence)	NM_174868.4
Accession Version	NM_174868.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	mitoguardin 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK085017.1, BC098205.1 and AC123075.5. On Jun 20, 2009 this sequence version replaced NM_174868.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK085017.1, SRR1660811.177667.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGACG AGACAGTCAG CAGATCACAG TTCTCCTTGA AGACGTACGC AGTTAGAGTG 
TTTGCTCTCC CTGTGTCTTG GTATTATTCT CTCTCCCAGA TCAAATTTTC ACCAGTAGCT 
AAGAAGCTGT TCATGGTAAC TGCCGTGAGT GCTGTGTCTG TGATTTTTCT GGCCCATCAC 
TTTAAAAGAA GACGGGGAAA ACAGAAGGGC AAAGTGCTGC CCTGGGAACC CGAGCACCTC 
CTCCTTGAGC ACACTAGGAG AGCTGCATCA GAAAAAGGTT CGAGTTGCTC CAGCAGTCGG 
CAGAATCTGA CCTTGTCGTT AAGCTCCACC AAGGAGAAAG GATCTCAGTG TTGCAACTAC 
CCGAATGGAG GGCTTCTCAG CAGATACTCG GGCTCTGCCC AGAGCCTGGG CTCCGTCCAG 
AGTGTTAACT CCTGTCATAG TTGTGCTTGT GGAAATTCTA ATTCCTGGGA CAAAGCAGAC 
GATGATGACA TCAGACTTGT TAACATTCCT GTGACCACAC CTGAGAACTT ATACCTGATG 
GGTATGGAAT TATTTGAAGA GGCGTTACGA CGATGGGAAC AAGCTCTGAC TTTTCGAAGT 
AGACAGGCTG AAGATGAAGC CTGCAGCTCT GTTAAACTGG GAGCCGGAGA TGCCATTGCA 
GAAGAAAGTG TGGATGATAT TATTAGTTCA GAATTCATCC ATAAACTTGA AGCTCTACTG 
CAAAGAGCGT ATCGTCTCCA AGAGGAGTTT GAGGCTACCC TTGGGGGATC TGACCCTAAT 
TCCATTGCAA ATGATACTGA TAAAGACACA GACATGAGCT TGAGGGAGAC TATGGATGAG 
CTTGGCCTGC CAGACGCCAT GAACATGGAC TCAGCAGATC TCTTTGCTTC AGCAACAGAG 
CTCGCAGAGC AGAGAGAAGC GCAGCAGACC TTCAGCCTGG AGTCTTTTTG TCCCTGCCCT 
TTCTACGAAG AAGCTATGCA TTTAGTTGAA GAAGGAAAAA TTTACTCCAG AGTGCTGAGA 
ACGGAGATGC TGGAGTGCCT GGGAGACAGT GACTTCCTCG CCAAACTTCA CTGTATCCGG 
CAGGCTTTTC AGCTAATCCT TGCAGAAGCA GACAACAGGT CATTCCTTGC TGAAAGTGGA 
AGGAAAATTT TGTCAGCTTT AATTGTGAAA GCACGGAAGA ATCCAAAGAA ATTTCAAGAT 
GTTTTTGATG AAATGATCAA TTTTTTAGAG CAGACTGATC ACTGGGATAG CACTGAGCTG 
GAACTTGCTG CCAGAGGGGT GAAAAATTTA AATTTTTATG ACGTGGTTCT GGATTTTATA 
TTAATGGATT CCTTTGAAGA TTTAGAAAAC CCACCGACAT CCATACAGAG CGTAGTGAAC 
AATCGCTGGC TCAACTCTTC CTTCAAAGAA AGCGCTGTGG CTTCAAGTTG TTGGTCAGTG 
CTGAAACAGA AAAGGCAACA GATGAAGATC TCAGATGGAT TTTTCGCTCA TTTTTATGCC 
ATTTGTGAGC ATGTCAGCCC TGTTTTAGCC TGGGGCTTCT TGGGTCCTAG AAATTCTCTC 
TATGACTTAT GTTGCTTCTT TAAGAATCAA GTTCTTTTCT TTCTCAAAGA CATCTTCGAC 
TTTGAGAAGG TGCGCTATTC GAGCATAGAC ACGCTGGCGG AGGACCTCAC GCACCTACTC 
ATCCGCCGCA CGGAGCTGCT GGTCACCTGT CTTGGTGCAG ATGCCCTGAG GCACGCCACC 
ACTTGCACGA GTGGACACTC CCACGCCGTG CCCACTGCGC TCTTAGAAGC CAAAGTACAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777357.2
CDS	377..2179
Translation	MSDETVSRSQFSLKTYAVRVFALPVSWYYSLSQIKFSPVAKKLFMVTAVSAVSVIFLAHHFKRRRGKQKGKVLPWEPEHLLLEHTRRAASEKGSSCSSSRQNLTLSLSSTKEKGSQCCNYPNGGLLSRYSGSAQSLGSVQSVNSCHSCACGNSNSWDKADDDDIRLVNIPVTTPENLYLMGMELFEEALRRWEQALTFRSRQAEDEACSSVKLGAGDAIAEESVDDIISSEFIHKLEALLQRAYRLQEEFEATLGGSDPNSIANDTDKDTDMSLRETMDELGLPDAMNMDSADLFASATELAEQREAQQTFSLESFCPCPFYEEAMHLVEEGKIYSRVLRTEMLECLGDSDFLAKLHCIRQAFQLILAEADNRSFLAESGRKILSALIVKARKNPKKFQDVFDEMINFLEQTDHWDSTELELAARGVKNLNFYDVVLDFILMDSFEDLENPPTSIQSVVNNRWLNSSFKESAVASSCWSVLKQKRQQMKISDGFFAHFYAICEHVSPVLAWGFLGPRNSLYDLCCFFKNQVLFFLKDIFDFEKVRYSSIDTLAEDLTHLLIRRTELLVTCLGADALRHATTCTSGHSHAVPTALLEAKVQ

Target ORF information:

RefSeq Version	NM_174868.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitoguardin 1 (Miga1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174868.4

ORF Insert Sequence:

ATGTCAGACG AGACAGTCAG CAGATCACAG TTCTCCTTGA AGACGTACGC AGTTAGAGTG 
TTTGCTCTCC CTGTGTCTTG GTATTATTCT CTCTCCCAGA TCAAATTTTC ACCAGTAGCT 
AAGAAGCTGT TCATGGTAAC TGCCGTGAGT GCTGTGTCTG TGATTTTTCT GGCCCATCAC 
TTTAAAAGAA GACGGGGAAA ACAGAAGGGC AAAGTGCTGC CCTGGGAACC CGAGCACCTC 
CTCCTTGAGC ACACTAGGAG AGCTGCATCA GAAAAAGGTT CGAGTTGCTC CAGCAGTCGG 
CAGAATCTGA CCTTGTCGTT AAGCTCCACC AAGGAGAAAG GATCTCAGTG TTGCAACTAC 
CCGAATGGAG GGCTTCTCAG CAGATACTCG GGCTCTGCCC AGAGCCTGGG CTCCGTCCAG 
AGTGTTAACT CCTGTCATAG TTGTGCTTGT GGAAATTCTA ATTCCTGGGA CAAAGCAGAC 
GATGATGACA TCAGACTTGT TAACATTCCT GTGACCACAC CTGAGAACTT ATACCTGATG 
GGTATGGAAT TATTTGAAGA GGCGTTACGA CGATGGGAAC AAGCTCTGAC TTTTCGAAGT 
AGACAGGCTG AAGATGAAGC CTGCAGCTCT GTTAAACTGG GAGCCGGAGA TGCCATTGCA 
GAAGAAAGTG TGGATGATAT TATTAGTTCA GAATTCATCC ATAAACTTGA AGCTCTACTG 
CAAAGAGCGT ATCGTCTCCA AGAGGAGTTT GAGGCTACCC TTGGGGGATC TGACCCTAAT 
TCCATTGCAA ATGATACTGA TAAAGACACA GACATGAGCT TGAGGGAGAC TATGGATGAG 
CTTGGCCTGC CAGACGCCAT GAACATGGAC TCAGCAGATC TCTTTGCTTC AGCAACAGAG 
CTCGCAGAGC AGAGAGAAGC GCAGCAGACC TTCAGCCTGG AGTCTTTTTG TCCCTGCCCT 
TTCTACGAAG AAGCTATGCA TTTAGTTGAA GAAGGAAAAA TTTACTCCAG AGTGCTGAGA 
ACGGAGATGC TGGAGTGCCT GGGAGACAGT GACTTCCTCG CCAAACTTCA CTGTATCCGG 
CAGGCTTTTC AGCTAATCCT TGCAGAAGCA GACAACAGGT CATTCCTTGC TGAAAGTGGA 
AGGAAAATTT TGTCAGCTTT AATTGTGAAA GCACGGAAGA ATCCAAAGAA ATTTCAAGAT 
GTTTTTGATG AAATGATCAA TTTTTTAGAG CAGACTGATC ACTGGGATAG CACTGAGCTG 
GAACTTGCTG CCAGAGGGGT GAAAAATTTA AATTTTTATG ACGTGGTTCT GGATTTTATA 
TTAATGGATT CCTTTGAAGA TTTAGAAAAC CCACCGACAT CCATACAGAG CGTAGTGAAC 
AATCGCTGGC TCAACTCTTC CTTCAAAGAA AGCGCTGTGG CTTCAAGTTG TTGGTCAGTG 
CTGAAACAGA AAAGGCAACA GATGAAGATC TCAGATGGAT TTTTCGCTCA TTTTTATGCC 
ATTTGTGAGC ATGTCAGCCC TGTTTTAGCC TGGGGCTTCT TGGGTCCTAG AAATTCTCTC 
TATGACTTAT GTTGCTTCTT TAAGAATCAA GTTCTTTTCT TTCTCAAAGA CATCTTCGAC 
TTTGAGAAGG TGCGCTATTC GAGCATAGAC ACGCTGGCGG AGGACCTCAC GCACCTACTC 
ATCCGCCGCA CGGAGCTGCT GGTCACCTGT CTTGGTGCAG ATGCCCTGAG GCACGCCACC 
ACTTGCACGA GTGGACACTC CCACGCCGTG CCCACTGCGC TCTTAGAAGC CAAAGTACAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Miga1 ( NM_001162375.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155847.1 Mus musculus mitoguardin 1 (Miga1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu08363
Clone ID Related Accession (Same CDS sequence)	NM_001162375.1
Accession Version	NM_001162375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	mitoguardin 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK085017.1, AK045347.1 and AC123075.5. Transcript Variant: This variant (2) lacks two exons in the coding region compared to variant 1. The encoded isoform (2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK045347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGACG AGACAGTCAG CAGATCACAG TTCTCCTTGA AGACGTACGC AGTTAGAGTG 
TTTGCTCTCC CTGTGTCTTG GTATTATTCT CTCTCCCAGA TCAAATTTTC ACCAGTAGCT 
AAGAAGCTGT TCATGGTAAC TGCCGTGAGT GCTGTGTCTG TGATTTTTCT GGCCCATCAC 
TTTAAAAGAA GACGGGGAAA ACAGAAGGGC AAAGTGCTGC CCTGGGAACC CGAGCACCTC 
CTCCTTGAGC ACACTAGGAG AGCTGCATCA GAAAAAGGTT CGAGTTGCTC CAGCAGTCGG 
CAGAATCTGA CCTTGTCGTT AAGCTCCACC AAGGAGAAAG GATCTCAGTG TTGCAACTAC 
CCGAATGGAG GGCTTCTCAG CAGATACTCG GGCTCTGCCC AGAGCCTGGG CTCCGTCCAG 
AGTGTTAACT CCTGTCATAG TTGTGCTTGT GGAAATTCTA ATTCCTGGGA CAAAGCAGAC 
GATGATGACA TCAGACTTGT TAACATTCCT GTGACCACAC CTGAGAACTT ATACCTGATG 
GGTATGGAAT TATTTGAAGA GGCGTTACGA CGATGGGAAC AAGCTCTGAC TTTTCGAAGT 
AGACAGGCTG AAGATGAAGC CTGCAGCTCT GTTAAACTGG GAGCCGGAGA TGCCATTGCA 
GAAGAAAGTG TGGATGATAT TATTAGTTCA GAATTCATCC ATAAACTTGA AGCTCTACTG 
CAAAGAGCGT ATCGTCTCCA AGAGGAGTTT GAGGCTACCC TTGGGGGATC TGACCCTAAT 
TCCATTGCAA ATGATACTGA TAAAGACACA GACATGAGCT TGAGGGAGAC TATGGATGAG 
CTTGGCCTGC CAGACGCCAT GAACATGGAC TCAGCAGATC TCTTTGCTTC AGCAACAGAG 
CTAATCCTTG CAGAAGCAGA CAACAGGTCA TTCCTTGCTG AAAGTGGAAG GAAAATTTTG 
TCAGCTTTAA TTGTGAAAGC ACGGAAGAAT CCAAAGAAAT TTCAAGATGT TTTTGATGAA 
ATGATCAATT TTTTAGAGCA GACTGATCAC TGGGATAGCA CTGAGCTGGA ACTTGCTGCC 
AGAGGGGTGA AAAATTTAAA TTTTTATGAC GTGGTTCTGG ATTTTATATT AATGGATTCC 
TTTGAAGATT TAGAAAACCC ACCGACATCC ATACAGAGCG TAGTGAACAA TCGCTGGCTC 
AACTCTTCCT TCAAAGAAAG CGCTGTGGCT TCAAGTTGTT GGTCAGTGCT GAAACAGAAA 
AGGCAACAGA TGAAGATCTC AGATGGATTT TTCGCTCATT TTTATGCCAT TTGTGAGCAT 
GTCAGCCCTG TTTTAGCCTG GGGCTTCTTG GGTCCTAGAA ATTCTCTCTA TGACTTATGT 
TGCTTCTTTA AGAATCAAGT TCTTTTCTTT CTCAAAGACA TCTTCGACTT TGAGAAGGTG 
CGCTATTCGA GCATAGACAC GCTGGCGGAG GACCTCACGC ACCTACTCAT CCGCCGCACG 
GAGCTGCTGG TCACCTGTCT TGGTGCAGAT GCCCTGAGGC ACGCCACCAC TTGCACGAGT 
GGACACTCCC ACGCCGTGCC CACTGCGCTC TTAGAAGCCA AAGTACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155847.1
CDS	377..1987
Translation	MSDETVSRSQFSLKTYAVRVFALPVSWYYSLSQIKFSPVAKKLFMVTAVSAVSVIFLAHHFKRRRGKQKGKVLPWEPEHLLLEHTRRAASEKGSSCSSSRQNLTLSLSSTKEKGSQCCNYPNGGLLSRYSGSAQSLGSVQSVNSCHSCACGNSNSWDKADDDDIRLVNIPVTTPENLYLMGMELFEEALRRWEQALTFRSRQAEDEACSSVKLGAGDAIAEESVDDIISSEFIHKLEALLQRAYRLQEEFEATLGGSDPNSIANDTDKDTDMSLRETMDELGLPDAMNMDSADLFASATELILAEADNRSFLAESGRKILSALIVKARKNPKKFQDVFDEMINFLEQTDHWDSTELELAARGVKNLNFYDVVLDFILMDSFEDLENPPTSIQSVVNNRWLNSSFKESAVASSCWSVLKQKRQQMKISDGFFAHFYAICEHVSPVLAWGFLGPRNSLYDLCCFFKNQVLFFLKDIFDFEKVRYSSIDTLAEDLTHLLIRRTELLVTCLGADALRHATTCTSGHSHAVPTALLEAKVQ

Target ORF information:

RefSeq Version	NM_001162375.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitoguardin 1 (Miga1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001162375.1

ORF Insert Sequence:

ATGTCAGACG AGACAGTCAG CAGATCACAG TTCTCCTTGA AGACGTACGC AGTTAGAGTG 
TTTGCTCTCC CTGTGTCTTG GTATTATTCT CTCTCCCAGA TCAAATTTTC ACCAGTAGCT 
AAGAAGCTGT TCATGGTAAC TGCCGTGAGT GCTGTGTCTG TGATTTTTCT GGCCCATCAC 
TTTAAAAGAA GACGGGGAAA ACAGAAGGGC AAAGTGCTGC CCTGGGAACC CGAGCACCTC 
CTCCTTGAGC ACACTAGGAG AGCTGCATCA GAAAAAGGTT CGAGTTGCTC CAGCAGTCGG 
CAGAATCTGA CCTTGTCGTT AAGCTCCACC AAGGAGAAAG GATCTCAGTG TTGCAACTAC 
CCGAATGGAG GGCTTCTCAG CAGATACTCG GGCTCTGCCC AGAGCCTGGG CTCCGTCCAG 
AGTGTTAACT CCTGTCATAG TTGTGCTTGT GGAAATTCTA ATTCCTGGGA CAAAGCAGAC 
GATGATGACA TCAGACTTGT TAACATTCCT GTGACCACAC CTGAGAACTT ATACCTGATG 
GGTATGGAAT TATTTGAAGA GGCGTTACGA CGATGGGAAC AAGCTCTGAC TTTTCGAAGT 
AGACAGGCTG AAGATGAAGC CTGCAGCTCT GTTAAACTGG GAGCCGGAGA TGCCATTGCA 
GAAGAAAGTG TGGATGATAT TATTAGTTCA GAATTCATCC ATAAACTTGA AGCTCTACTG 
CAAAGAGCGT ATCGTCTCCA AGAGGAGTTT GAGGCTACCC TTGGGGGATC TGACCCTAAT 
TCCATTGCAA ATGATACTGA TAAAGACACA GACATGAGCT TGAGGGAGAC TATGGATGAG 
CTTGGCCTGC CAGACGCCAT GAACATGGAC TCAGCAGATC TCTTTGCTTC AGCAACAGAG 
CTAATCCTTG CAGAAGCAGA CAACAGGTCA TTCCTTGCTG AAAGTGGAAG GAAAATTTTG 
TCAGCTTTAA TTGTGAAAGC ACGGAAGAAT CCAAAGAAAT TTCAAGATGT TTTTGATGAA 
ATGATCAATT TTTTAGAGCA GACTGATCAC TGGGATAGCA CTGAGCTGGA ACTTGCTGCC 
AGAGGGGTGA AAAATTTAAA TTTTTATGAC GTGGTTCTGG ATTTTATATT AATGGATTCC 
TTTGAAGATT TAGAAAACCC ACCGACATCC ATACAGAGCG TAGTGAACAA TCGCTGGCTC 
AACTCTTCCT TCAAAGAAAG CGCTGTGGCT TCAAGTTGTT GGTCAGTGCT GAAACAGAAA 
AGGCAACAGA TGAAGATCTC AGATGGATTT TTCGCTCATT TTTATGCCAT TTGTGAGCAT 
GTCAGCCCTG TTTTAGCCTG GGGCTTCTTG GGTCCTAGAA ATTCTCTCTA TGACTTATGT 
TGCTTCTTTA AGAATCAAGT TCTTTTCTTT CTCAAAGACA TCTTCGACTT TGAGAAGGTG 
CGCTATTCGA GCATAGACAC GCTGGCGGAG GACCTCACGC ACCTACTCAT CCGCCGCACG 
GAGCTGCTGG TCACCTGTCT TGGTGCAGAT GCCCTGAGGC ACGCCACCAC TTGCACGAGT 
GGACACTCCC ACGCCGTGCC CACTGCGCTC TTAGAAGCCA AAGTACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Miga1 ( XM_011240070.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238372.1 Mus musculus family with sequence similarity 73, member A (Fam73a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu72934
Clone ID Related Accession (Same CDS sequence)	XM_011240070.2 , XM_011240070.3
Accession Version	XM_011240070.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitoguardin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240070.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACG AGACAGTCAG CAGATCACAG TTCTCCTTGA AGACGTACGC AGTTAGAGTG 
TTTGCTCTCC CTGTGTCTTG GTATTATTCT CTCTCCCAGA TCAAATTTTC ACCAGTAGCT 
AAGAAGCTGT TCATGGTAAC TGCCGTGAGT GCTGTGTCTG TGATTTTTCT GGCCCATCAC 
TTTAAAAGAA GACGGGGAAA ACAGAAGGGC AAAGTGCTGC CCTGGGAACC CGAGCACCTC 
CTCCTTGAGC ACACTAGGAG AGCTGCATCA GAAAAAGGTT CGAGTTGCTC CAGCAGTCGG 
CAGAATCTGA CCTTGTCGTT AAGCTCCACC AAGGAGAAAG GATCTCAGTG TTGCAACTAC 
CCGAATGGAG GGCTTCTCAG CAGATACTCG GGCTCTGCCC AGAGCCTGGG CTCCGTCCAG 
AGTGTTAACT CCTGTCATAG TTGTGCTTGT GGAAATTCTA ATTCCTGGGA CAAAGCAGAC 
GATGATGACA TCAGACTTGT TAACATTCCT GTGACCACAC CTGAGAACTT ATACCTGATG 
GGTATGGAAT TATTTGAAGA GGCGTTACGA CGATGGGAAC AAGCTCTGAC TTTTCGAAGT 
AGACAGGCTG AAGATGAAGC CTGCAGCTCT GTTAAACTGG GAGCCGGAGA TGCCATTGCA 
GAAGAAAGTG TGGATGATAT TATTAGTTCA GAATTCATCC ATAAACTTGA AGCTCTACTG 
CAAAGAGCGT ATCGTCTCCA AGAGGAGTTT GAGGCTACCC TTGGGGGATC TGACCCTAAT 
TCCATTGCAA ATGATACTGA TAAAGACACA GACATGAGCT TGAGGGAGAC TATGGATGAG 
CTTGGCCTGC CAGACGCCAT GAACATGGAC TCAGCAGATC TCTTTGCTTC AGCAACAGAG 
CTCGCAGAGC AGAGAGAAGC GCAGCAGACC TTCAGCCTGG AGTCTTTTTG TCCCTGCCCT 
TTCTACGAAG AAGCTATGCA TTTAGTTGAA GAAGGAAAAA TTTACTCCAG AGTGCTGAGA 
ACGGAGATGC TGGAGTGCCT GGGAGACAGT GACTTCCTCG CCAAACTTCA CTGTATCCGG 
CAGGCTTTTC AGCTAATCCT TGCAGAAGCA GACAACAGGT CATTCCTTGC TGAAAGTGGA 
AGGAAAATTT TGTCAGCTTT AATTGTGAAA GCACGGAAGA ATCCAAAGAA ATTTCAAGAT 
GTTTTTGATG AAATGATCAA TTTTTTAGAG CAGACTGATC ACTGGGATAG CACTGAGCTG 
GAACTTGCTG CCAGAGGGGT GAAAAATTTA AATTTTTATG ACGTGGTTCT GGATTTTATA 
TTAATGGATT CCTTTGAAGA TTTAGAAAAC CCACCGACAT CCATACAGAG CGTAGTGAAC 
AATCGCTGGC TCAACTCTTC CTTCAAAGAA AGCGCTGTGG CTTCAAGTTG TTGGTCAGTG 
CTGAAACAGA AAAGGCAACA GATGAAGAAA AGAGAGGAGG CATCGGATCT CTCTGAGACT 
GGAGTTAGAG AACTACCATG TCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238372.1
CDS	393..1922
Translation	MSDETVSRSQFSLKTYAVRVFALPVSWYYSLSQIKFSPVAKKLFMVTAVSAVSVIFLAHHFKRRRGKQKGKVLPWEPEHLLLEHTRRAASEKGSSCSSSRQNLTLSLSSTKEKGSQCCNYPNGGLLSRYSGSAQSLGSVQSVNSCHSCACGNSNSWDKADDDDIRLVNIPVTTPENLYLMGMELFEEALRRWEQALTFRSRQAEDEACSSVKLGAGDAIAEESVDDIISSEFIHKLEALLQRAYRLQEEFEATLGGSDPNSIANDTDKDTDMSLRETMDELGLPDAMNMDSADLFASATELAEQREAQQTFSLESFCPCPFYEEAMHLVEEGKIYSRVLRTEMLECLGDSDFLAKLHCIRQAFQLILAEADNRSFLAESGRKILSALIVKARKNPKKFQDVFDEMINFLEQTDHWDSTELELAARGVKNLNFYDVVLDFILMDSFEDLENPPTSIQSVVNNRWLNSSFKESAVASSCWSVLKQKRQQMKKREEASDLSETGVRELPCQR

Target ORF information:

RefSeq Version	XM_011240070.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus family with sequence similarity 73, member A (Fam73a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240070.2

ORF Insert Sequence:

ATGTCAGACG AGACAGTCAG CAGATCACAG TTCTCCTTGA AGACGTACGC AGTTAGAGTG 
TTTGCTCTCC CTGTGTCTTG GTATTATTCT CTCTCCCAGA TCAAATTTTC ACCAGTAGCT 
AAGAAGCTGT TCATGGTAAC TGCCGTGAGT GCTGTGTCTG TGATTTTTCT GGCCCATCAC 
TTTAAAAGAA GACGGGGAAA ACAGAAGGGC AAAGTGCTGC CCTGGGAACC CGAGCACCTC 
CTCCTTGAGC ACACTAGGAG AGCTGCATCA GAAAAAGGTT CGAGTTGCTC CAGCAGTCGG 
CAGAATCTGA CCTTGTCGTT AAGCTCCACC AAGGAGAAAG GATCTCAGTG TTGCAACTAC 
CCGAATGGAG GGCTTCTCAG CAGATACTCG GGCTCTGCCC AGAGCCTGGG CTCCGTCCAG 
AGTGTTAACT CCTGTCATAG TTGTGCTTGT GGAAATTCTA ATTCCTGGGA CAAAGCAGAC 
GATGATGACA TCAGACTTGT TAACATTCCT GTGACCACAC CTGAGAACTT ATACCTGATG 
GGTATGGAAT TATTTGAAGA GGCGTTACGA CGATGGGAAC AAGCTCTGAC TTTTCGAAGT 
AGACAGGCTG AAGATGAAGC CTGCAGCTCT GTTAAACTGG GAGCCGGAGA TGCCATTGCA 
GAAGAAAGTG TGGATGATAT TATTAGTTCA GAATTCATCC ATAAACTTGA AGCTCTACTG 
CAAAGAGCGT ATCGTCTCCA AGAGGAGTTT GAGGCTACCC TTGGGGGATC TGACCCTAAT 
TCCATTGCAA ATGATACTGA TAAAGACACA GACATGAGCT TGAGGGAGAC TATGGATGAG 
CTTGGCCTGC CAGACGCCAT GAACATGGAC TCAGCAGATC TCTTTGCTTC AGCAACAGAG 
CTCGCAGAGC AGAGAGAAGC GCAGCAGACC TTCAGCCTGG AGTCTTTTTG TCCCTGCCCT 
TTCTACGAAG AAGCTATGCA TTTAGTTGAA GAAGGAAAAA TTTACTCCAG AGTGCTGAGA 
ACGGAGATGC TGGAGTGCCT GGGAGACAGT GACTTCCTCG CCAAACTTCA CTGTATCCGG 
CAGGCTTTTC AGCTAATCCT TGCAGAAGCA GACAACAGGT CATTCCTTGC TGAAAGTGGA 
AGGAAAATTT TGTCAGCTTT AATTGTGAAA GCACGGAAGA ATCCAAAGAA ATTTCAAGAT 
GTTTTTGATG AAATGATCAA TTTTTTAGAG CAGACTGATC ACTGGGATAG CACTGAGCTG 
GAACTTGCTG CCAGAGGGGT GAAAAATTTA AATTTTTATG ACGTGGTTCT GGATTTTATA 
TTAATGGATT CCTTTGAAGA TTTAGAAAAC CCACCGACAT CCATACAGAG CGTAGTGAAC 
AATCGCTGGC TCAACTCTTC CTTCAAAGAA AGCGCTGTGG CTTCAAGTTG TTGGTCAGTG 
CTGAAACAGA AAAGGCAACA GATGAAGAAA AGAGAGGAGG CATCGGATCT CTCTGAGACT 
GGAGTTAGAG AACTACCATG TCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Miga1 ( XM_011240070.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238372.1 Mus musculus mitoguardin 1 (Miga1), transcript variant X1, mRNA.

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CloneID	OMu72934
Clone ID Related Accession (Same CDS sequence)	XM_011240070.2 , XM_011240070.3
Accession Version	XM_011240070.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitoguardin 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240070.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACG AGACAGTCAG CAGATCACAG TTCTCCTTGA AGACGTACGC AGTTAGAGTG 
TTTGCTCTCC CTGTGTCTTG GTATTATTCT CTCTCCCAGA TCAAATTTTC ACCAGTAGCT 
AAGAAGCTGT TCATGGTAAC TGCCGTGAGT GCTGTGTCTG TGATTTTTCT GGCCCATCAC 
TTTAAAAGAA GACGGGGAAA ACAGAAGGGC AAAGTGCTGC CCTGGGAACC CGAGCACCTC 
CTCCTTGAGC ACACTAGGAG AGCTGCATCA GAAAAAGGTT CGAGTTGCTC CAGCAGTCGG 
CAGAATCTGA CCTTGTCGTT AAGCTCCACC AAGGAGAAAG GATCTCAGTG TTGCAACTAC 
CCGAATGGAG GGCTTCTCAG CAGATACTCG GGCTCTGCCC AGAGCCTGGG CTCCGTCCAG 
AGTGTTAACT CCTGTCATAG TTGTGCTTGT GGAAATTCTA ATTCCTGGGA CAAAGCAGAC 
GATGATGACA TCAGACTTGT TAACATTCCT GTGACCACAC CTGAGAACTT ATACCTGATG 
GGTATGGAAT TATTTGAAGA GGCGTTACGA CGATGGGAAC AAGCTCTGAC TTTTCGAAGT 
AGACAGGCTG AAGATGAAGC CTGCAGCTCT GTTAAACTGG GAGCCGGAGA TGCCATTGCA 
GAAGAAAGTG TGGATGATAT TATTAGTTCA GAATTCATCC ATAAACTTGA AGCTCTACTG 
CAAAGAGCGT ATCGTCTCCA AGAGGAGTTT GAGGCTACCC TTGGGGGATC TGACCCTAAT 
TCCATTGCAA ATGATACTGA TAAAGACACA GACATGAGCT TGAGGGAGAC TATGGATGAG 
CTTGGCCTGC CAGACGCCAT GAACATGGAC TCAGCAGATC TCTTTGCTTC AGCAACAGAG 
CTCGCAGAGC AGAGAGAAGC GCAGCAGACC TTCAGCCTGG AGTCTTTTTG TCCCTGCCCT 
TTCTACGAAG AAGCTATGCA TTTAGTTGAA GAAGGAAAAA TTTACTCCAG AGTGCTGAGA 
ACGGAGATGC TGGAGTGCCT GGGAGACAGT GACTTCCTCG CCAAACTTCA CTGTATCCGG 
CAGGCTTTTC AGCTAATCCT TGCAGAAGCA GACAACAGGT CATTCCTTGC TGAAAGTGGA 
AGGAAAATTT TGTCAGCTTT AATTGTGAAA GCACGGAAGA ATCCAAAGAA ATTTCAAGAT 
GTTTTTGATG AAATGATCAA TTTTTTAGAG CAGACTGATC ACTGGGATAG CACTGAGCTG 
GAACTTGCTG CCAGAGGGGT GAAAAATTTA AATTTTTATG ACGTGGTTCT GGATTTTATA 
TTAATGGATT CCTTTGAAGA TTTAGAAAAC CCACCGACAT CCATACAGAG CGTAGTGAAC 
AATCGCTGGC TCAACTCTTC CTTCAAAGAA AGCGCTGTGG CTTCAAGTTG TTGGTCAGTG 
CTGAAACAGA AAAGGCAACA GATGAAGAAA AGAGAGGAGG CATCGGATCT CTCTGAGACT 
GGAGTTAGAG AACTACCATG TCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238372.1
CDS	406..1935
Translation	MSDETVSRSQFSLKTYAVRVFALPVSWYYSLSQIKFSPVAKKLFMVTAVSAVSVIFLAHHFKRRRGKQKGKVLPWEPEHLLLEHTRRAASEKGSSCSSSRQNLTLSLSSTKEKGSQCCNYPNGGLLSRYSGSAQSLGSVQSVNSCHSCACGNSNSWDKADDDDIRLVNIPVTTPENLYLMGMELFEEALRRWEQALTFRSRQAEDEACSSVKLGAGDAIAEESVDDIISSEFIHKLEALLQRAYRLQEEFEATLGGSDPNSIANDTDKDTDMSLRETMDELGLPDAMNMDSADLFASATELAEQREAQQTFSLESFCPCPFYEEAMHLVEEGKIYSRVLRTEMLECLGDSDFLAKLHCIRQAFQLILAEADNRSFLAESGRKILSALIVKARKNPKKFQDVFDEMINFLEQTDHWDSTELELAARGVKNLNFYDVVLDFILMDSFEDLENPPTSIQSVVNNRWLNSSFKESAVASSCWSVLKQKRQQMKKREEASDLSETGVRELPCQR

Target ORF information:

RefSeq Version	XM_011240070.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitoguardin 1 (Miga1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240070.3

ORF Insert Sequence:

ATGTCAGACG AGACAGTCAG CAGATCACAG TTCTCCTTGA AGACGTACGC AGTTAGAGTG 
TTTGCTCTCC CTGTGTCTTG GTATTATTCT CTCTCCCAGA TCAAATTTTC ACCAGTAGCT 
AAGAAGCTGT TCATGGTAAC TGCCGTGAGT GCTGTGTCTG TGATTTTTCT GGCCCATCAC 
TTTAAAAGAA GACGGGGAAA ACAGAAGGGC AAAGTGCTGC CCTGGGAACC CGAGCACCTC 
CTCCTTGAGC ACACTAGGAG AGCTGCATCA GAAAAAGGTT CGAGTTGCTC CAGCAGTCGG 
CAGAATCTGA CCTTGTCGTT AAGCTCCACC AAGGAGAAAG GATCTCAGTG TTGCAACTAC 
CCGAATGGAG GGCTTCTCAG CAGATACTCG GGCTCTGCCC AGAGCCTGGG CTCCGTCCAG 
AGTGTTAACT CCTGTCATAG TTGTGCTTGT GGAAATTCTA ATTCCTGGGA CAAAGCAGAC 
GATGATGACA TCAGACTTGT TAACATTCCT GTGACCACAC CTGAGAACTT ATACCTGATG 
GGTATGGAAT TATTTGAAGA GGCGTTACGA CGATGGGAAC AAGCTCTGAC TTTTCGAAGT 
AGACAGGCTG AAGATGAAGC CTGCAGCTCT GTTAAACTGG GAGCCGGAGA TGCCATTGCA 
GAAGAAAGTG TGGATGATAT TATTAGTTCA GAATTCATCC ATAAACTTGA AGCTCTACTG 
CAAAGAGCGT ATCGTCTCCA AGAGGAGTTT GAGGCTACCC TTGGGGGATC TGACCCTAAT 
TCCATTGCAA ATGATACTGA TAAAGACACA GACATGAGCT TGAGGGAGAC TATGGATGAG 
CTTGGCCTGC CAGACGCCAT GAACATGGAC TCAGCAGATC TCTTTGCTTC AGCAACAGAG 
CTCGCAGAGC AGAGAGAAGC GCAGCAGACC TTCAGCCTGG AGTCTTTTTG TCCCTGCCCT 
TTCTACGAAG AAGCTATGCA TTTAGTTGAA GAAGGAAAAA TTTACTCCAG AGTGCTGAGA 
ACGGAGATGC TGGAGTGCCT GGGAGACAGT GACTTCCTCG CCAAACTTCA CTGTATCCGG 
CAGGCTTTTC AGCTAATCCT TGCAGAAGCA GACAACAGGT CATTCCTTGC TGAAAGTGGA 
AGGAAAATTT TGTCAGCTTT AATTGTGAAA GCACGGAAGA ATCCAAAGAA ATTTCAAGAT 
GTTTTTGATG AAATGATCAA TTTTTTAGAG CAGACTGATC ACTGGGATAG CACTGAGCTG 
GAACTTGCTG CCAGAGGGGT GAAAAATTTA AATTTTTATG ACGTGGTTCT GGATTTTATA 
TTAATGGATT CCTTTGAAGA TTTAGAAAAC CCACCGACAT CCATACAGAG CGTAGTGAAC 
AATCGCTGGC TCAACTCTTC CTTCAAAGAA AGCGCTGTGG CTTCAAGTTG TTGGTCAGTG 
CTGAAACAGA AAAGGCAACA GATGAAGAAA AGAGAGGAGG CATCGGATCT CTCTGAGACT 
GGAGTTAGAG AACTACCATG TCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial dynamics controls anti-tumour innate immunity by regulating CHIP-IRF1 axis stability.
Nature communications8(1)1805(2017 Nov)
Gao Z,Li Y,Wang F,Huang T,Fan K,Zhang Y,Zhong J,Cao Q,Chao T,Jia J,Yang S,Zhang L,Xiao Y,Zhou JY,Feng XH,Jin J

Mitochondrial Function Regulated by Mitoguardin-1/2 Is Crucial for Ovarian Endocrine Functions and Ovulation.
Endocrinology158(11)3988-3999(2017 Nov)
Liu XM,Zhang YL,Ji SY,Zhao LW,Shang WN,Li D,Chen Z,Tong C,Fan HY

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.
Molecular cell61(1)111-24(2016 Jan)
Zhang Y,Liu X,Bai J,Tian X,Zhao X,Liu W,Duan X,Shang W,Fan HY,Tong C

Mitoguardin-1 and -2 promote maturation and the developmental potential of mouse oocytes by maintaining mitochondrial dynamics and functions.
Oncotarget7(2)1155-67(2016 Jan)
Liu XM,Zhang YP,Ji SY,Li BT,Tian X,Li D,Tong C,Fan HY

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Miga1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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