Trim50 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim50
Entrez Gene ID	215061
Full Name	tripartite motif-containing 50
General protein information	Preferred Names tripartite motif-containing 50 Names E3 ubiquitin-protein ligase TRIM50 RING-type E3 ubiquitin transferase TRIM50 tripartite motif protein 50 tripartite motif-containing protein 50
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006504412.3	XP_006504475.1	E3 ubiquitin-protein ligase TRIM50 isoform X2
NM_178240.2	NP_839971.1	E3 ubiquitin-protein ligase TRIM50 isoform 1
NM_178240.3	NP_839971.1	E3 ubiquitin-protein ligase TRIM50 isoform 1
NM_001346745.1	NP_001333674.1	E3 ubiquitin-protein ligase TRIM50 isoform 2
XM_006504411.3	XP_006504474.1	E3 ubiquitin-protein ligase TRIM50 isoform X1
XM_006504411.4	XP_006504474.1	E3 ubiquitin-protein ligase TRIM50 isoform X1
XM_006504412.4	XP_006504475.1	E3 ubiquitin-protein ligase TRIM50 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TRIM50	NP_001193185.1
Canis lupus familiaris (dog)	TRIM50	XP_546929.2
Rattus norvegicus (Norway rat)	Trim50	NP_851594.1
Gallus gallus (chicken)	TRIM50	XP_415709.3
Homo sapiens (human)	TRIM50	NP_001268379.1
Mus musculus (house mouse)	Trim50	NP_839971.1
Pan troglodytes (chimpanzee)	TRIM50	XP_527777.2
	TRIM50	XP_001110857.1
Xenopus tropicalis (tropical clawed frog)	trim50	XP_004914939.1

Gene Ontology
Bibliography

Related articles in PubMed

TRIM50 protein regulates vesicular trafficking for acid secretion in gastric parietal cells.
Nishi M, Aoyama F, Kisa F, Zhu H, Sun M, Lin P, Ohta H, Van B, Yamamoto S, Kakizawa S, Sakai H, Ma J, Sawaguchi A, Takeshima H
The Journal of biological chemistry287(40)33523-32(2012 Sep)

The E3-ubiquitin ligase TRIM50 interacts with HDAC6 and p62, and promotes the sequestration and clearance of ubiquitinated proteins into the aggresome.
Fusco C, Micale L, Egorov M, Monti M, D'Addetta EV, Augello B, Cozzolino F, Calcagn? A, Fontana A, Polishchuk RS, Didelot G, Reymond A, Pucci P, Merla G
PloS one7(7)e40440(2012)

Induced chromosome deletions cause hypersociability and other features of Williams-Beuren syndrome in mice.
Li HH, Roy M, Kuscuoglu U, Spencer CM, Halm B, Harrison KC, Bayle JH, Splendore A, Ding F, Meltzer LA, Wright E, Paylor R, Deisseroth K, Francke U
EMBO molecular medicine1(1)50-65(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TRIM50 protein regulates vesicular trafficking for acid secretion in gastric parietal cells
Title: TRIM50 protein regulates vesicular trafficking for acid secretion in gastric parietal cells.

The following Trim50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07117	NM_178240.3 Latest version!	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu36138	NM_001346745.1 Latest version!	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu07117	XM_006504412.4 Latest version!	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu36138	XM_006504411.4 Latest version!	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu07117	NM_178240.2	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu36138	XM_006504411.3	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu07117	XM_006504412.3	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim50 ( NM_178240.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_839971.1 Mus musculus tripartite motif-containing 50 (Trim50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07117
Clone ID Related Accession (Same CDS sequence)	XM_006504412.3 , NM_178240.2 , NM_178240.3 , XM_006504412.4
Accession Version	NM_178240.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM50 isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC074359.2. On Nov 17, 2006 this sequence version replaced NM_178240.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY081947.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGTTCC ACTCTGTGGC CTCCAGGGCA GAGGTCCAGC AGGCTCGGCC TTTGGAAGGC 
GTCTTCAGTC CCATCTCCTT CAAGCCAGCT CTCCACCAGG CTGACATCAA ACTGACCGTG 
TGGAAGAGAC TCTTCCGGAA GGTTCTGCCA GCTCCAGCAT CTCTCAAGCT GGACCCGGCT 
ACTGCCCACC CTCTGCTGGA GCTCTCTAAG GGCAACACAG TGGTGCATTG TGGCCTCCTT 
GCCCAGAGAC GAGCCAGCCA ACCCGAGCGC TTCGACTATA GCACCTGTGT CCTGGCTAGC 
AAGGGCTTCT CTTGGGGTCG CCACTACTGG GAAGTGGTGG TGGGGAGCAA GAGTGACTGG 
CGCTTGGGGG TCATCAAGGG CACAGCCAGC CGCAAAGGCA AGCTGAACAA GTCCCCAGAG 
CACGGTGTGT GGCTAATTGG CCTGAAAGAG GGTCGGGTGT ATGAGGCTTT TGGCTGCCCA 
CGGCTGCCAC TGCCTGTGGC TGGCCACCCT CATCGCATCG GCGTCTACTT ACACTATGAG 
CAGGGTGAGC TCACGTTCTT TGACGCTGAC CGTCCTGATG ATCTGCGGAC ACTCTATACT 
TTCCAAGCTG ACTTCCAGGG CAAGCTGTAC CCTATCCTGG ACACATGTTG GCATGAGAGG 
GGCAGCAACT CACTGCCCAT GGTATTGCCC CCACCCAGTG GGCCTGGGCA CTTCACACTG 
GGACAGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_839971.1
CDS	1..1452
Translation	MAWRLTVPELQDQLQCPICLEVFKEPLMLQCGHSYCKDCLDNLSQHLDSELCCPVCRQSVDCSSSPPNVSLARVIDALRLPGDIEPTVCVHHRNPLSLFCEKDQEFICGLCGLLGSHQHHRVTPVSTVYSRMKEELAGRISELKEEHRNVEEHIGKLVNNRTRIINESDVFSWVIRREFQELHHLVDEEKARCLEGLEGHTRGLVASLDMQLEQAQGTQERLAQAEQVLEQFGNESHHEFIRFHSVASRAEVQQARPLEGVFSPISFKPALHQADIKLTVWKRLFRKVLPAPASLKLDPATAHPLLELSKGNTVVHCGLLAQRRASQPERFDYSTCVLASKGFSWGRHYWEVVVGSKSDWRLGVIKGTASRKGKLNKSPEHGVWLIGLKEGRVYEAFGCPRLPLPVAGHPHRIGVYLHYEQGELTFFDADRPDDLRTLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGHFTLGQV

Target ORF information:

RefSeq Version	NM_178240.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178240.2

ORF Insert Sequence:

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGTTCC ACTCTGTGGC CTCCAGGGCA GAGGTCCAGC AGGCTCGGCC TTTGGAAGGC 
GTCTTCAGTC CCATCTCCTT CAAGCCAGCT CTCCACCAGG CTGACATCAA ACTGACCGTG 
TGGAAGAGAC TCTTCCGGAA GGTTCTGCCA GCTCCAGCAT CTCTCAAGCT GGACCCGGCT 
ACTGCCCACC CTCTGCTGGA GCTCTCTAAG GGCAACACAG TGGTGCATTG TGGCCTCCTT 
GCCCAGAGAC GAGCCAGCCA ACCCGAGCGC TTCGACTATA GCACCTGTGT CCTGGCTAGC 
AAGGGCTTCT CTTGGGGTCG CCACTACTGG GAAGTGGTGG TGGGGAGCAA GAGTGACTGG 
CGCTTGGGGG TCATCAAGGG CACAGCCAGC CGCAAAGGCA AGCTGAACAA GTCCCCAGAG 
CACGGTGTGT GGCTAATTGG CCTGAAAGAG GGTCGGGTGT ATGAGGCTTT TGGCTGCCCA 
CGGCTGCCAC TGCCTGTGGC TGGCCACCCT CATCGCATCG GCGTCTACTT ACACTATGAG 
CAGGGTGAGC TCACGTTCTT TGACGCTGAC CGTCCTGATG ATCTGCGGAC ACTCTATACT 
TTCCAAGCTG ACTTCCAGGG CAAGCTGTAC CCTATCCTGG ACACATGTTG GCATGAGAGG 
GGCAGCAACT CACTGCCCAT GGTATTGCCC CCACCCAGTG GGCCTGGGCA CTTCACACTG 
GGACAGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim50 ( NM_178240.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_839971.1 Mus musculus tripartite motif-containing 50 (Trim50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07117
Clone ID Related Accession (Same CDS sequence)	XM_006504412.3 , NM_178240.2 , NM_178240.3 , XM_006504412.4
Accession Version	NM_178240.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM50 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074359.2. On Mar 1, 2018 this sequence version replaced NM_178240.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY081947.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGTTCC ACTCTGTGGC CTCCAGGGCA GAGGTCCAGC AGGCTCGGCC TTTGGAAGGC 
GTCTTCAGTC CCATCTCCTT CAAGCCAGCT CTCCACCAGG CTGACATCAA ACTGACCGTG 
TGGAAGAGAC TCTTCCGGAA GGTTCTGCCA GCTCCAGCAT CTCTCAAGCT GGACCCGGCT 
ACTGCCCACC CTCTGCTGGA GCTCTCTAAG GGCAACACAG TGGTGCATTG TGGCCTCCTT 
GCCCAGAGAC GAGCCAGCCA ACCCGAGCGC TTCGACTATA GCACCTGTGT CCTGGCTAGC 
AAGGGCTTCT CTTGGGGTCG CCACTACTGG GAAGTGGTGG TGGGGAGCAA GAGTGACTGG 
CGCTTGGGGG TCATCAAGGG CACAGCCAGC CGCAAAGGCA AGCTGAACAA GTCCCCAGAG 
CACGGTGTGT GGCTAATTGG CCTGAAAGAG GGTCGGGTGT ATGAGGCTTT TGGCTGCCCA 
CGGCTGCCAC TGCCTGTGGC TGGCCACCCT CATCGCATCG GCGTCTACTT ACACTATGAG 
CAGGGTGAGC TCACGTTCTT TGACGCTGAC CGTCCTGATG ATCTGCGGAC ACTCTATACT 
TTCCAAGCTG ACTTCCAGGG CAAGCTGTAC CCTATCCTGG ACACATGTTG GCATGAGAGG 
GGCAGCAACT CACTGCCCAT GGTATTGCCC CCACCCAGTG GGCCTGGGCA CTTCACACTG 
GGACAGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_839971.1
CDS	2..1453
Translation	MAWRLTVPELQDQLQCPICLEVFKEPLMLQCGHSYCKDCLDNLSQHLDSELCCPVCRQSVDCSSSPPNVSLARVIDALRLPGDIEPTVCVHHRNPLSLFCEKDQEFICGLCGLLGSHQHHRVTPVSTVYSRMKEELAGRISELKEEHRNVEEHIGKLVNNRTRIINESDVFSWVIRREFQELHHLVDEEKARCLEGLEGHTRGLVASLDMQLEQAQGTQERLAQAEQVLEQFGNESHHEFIRFHSVASRAEVQQARPLEGVFSPISFKPALHQADIKLTVWKRLFRKVLPAPASLKLDPATAHPLLELSKGNTVVHCGLLAQRRASQPERFDYSTCVLASKGFSWGRHYWEVVVGSKSDWRLGVIKGTASRKGKLNKSPEHGVWLIGLKEGRVYEAFGCPRLPLPVAGHPHRIGVYLHYEQGELTFFDADRPDDLRTLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGHFTLGQV

Target ORF information:

RefSeq Version	NM_178240.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178240.3

ORF Insert Sequence:

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGTTCC ACTCTGTGGC CTCCAGGGCA GAGGTCCAGC AGGCTCGGCC TTTGGAAGGC 
GTCTTCAGTC CCATCTCCTT CAAGCCAGCT CTCCACCAGG CTGACATCAA ACTGACCGTG 
TGGAAGAGAC TCTTCCGGAA GGTTCTGCCA GCTCCAGCAT CTCTCAAGCT GGACCCGGCT 
ACTGCCCACC CTCTGCTGGA GCTCTCTAAG GGCAACACAG TGGTGCATTG TGGCCTCCTT 
GCCCAGAGAC GAGCCAGCCA ACCCGAGCGC TTCGACTATA GCACCTGTGT CCTGGCTAGC 
AAGGGCTTCT CTTGGGGTCG CCACTACTGG GAAGTGGTGG TGGGGAGCAA GAGTGACTGG 
CGCTTGGGGG TCATCAAGGG CACAGCCAGC CGCAAAGGCA AGCTGAACAA GTCCCCAGAG 
CACGGTGTGT GGCTAATTGG CCTGAAAGAG GGTCGGGTGT ATGAGGCTTT TGGCTGCCCA 
CGGCTGCCAC TGCCTGTGGC TGGCCACCCT CATCGCATCG GCGTCTACTT ACACTATGAG 
CAGGGTGAGC TCACGTTCTT TGACGCTGAC CGTCCTGATG ATCTGCGGAC ACTCTATACT 
TTCCAAGCTG ACTTCCAGGG CAAGCTGTAC CCTATCCTGG ACACATGTTG GCATGAGAGG 
GGCAGCAACT CACTGCCCAT GGTATTGCCC CCACCCAGTG GGCCTGGGCA CTTCACACTG 
GGACAGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim50 ( NM_001346745.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333674.1 Mus musculus tripartite motif-containing 50 (Trim50), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu36138
Clone ID Related Accession (Same CDS sequence)	NM_001346745.1 , XM_006504411.3 , XM_006504411.4
Accession Version	NM_001346745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM50 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074359.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC130022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGAAGT TCCACTCTGT GGCCTCCAGG GCAGAGGTCC AGCAGGCTCG GCCTTTGGAA 
GGCGTCTTCA GTCCCATCTC CTTCAAGCCA GCTCTCCACC AGGCTGACAT CAAACTGACC 
GTGTGGAAGA GACTCTTCCG GAAGGTTCTG CCAGCTCCAG CATCTCTCAA GCTGGACCCG 
GCTACTGCCC ACCCTCTGCT GGAGCTCTCT AAGGGCAACA CAGTGGTGCA TTGTGGCCTC 
CTTGCCCAGA GACGAGCCAG CCAACCCGAG CGCTTCGACT ATAGCACCTG TGTCCTGGCT 
AGCAAGGGCT TCTCTTGGGG TCGCCACTAC TGGGAAGTGG TGGTGGGGAG CAAGAGTGAC 
TGGCGCTTGG GGGTCATCAA GGGCACAGCC AGCCGCAAAG GCAAGCTGAA CAAGTCCCCA 
GAGCACGGTG TGTGGCTAAT TGGCCTGAAA GAGGGTCGGG TGTATGAGGC TTTTGGCTGC 
CCACGGCTGC CACTGCCTGT GGCTGGCCAC CCTCATCGCA TCGGCGTCTA CTTACACTAT 
GAGCAGGGTG AGCTCACGTT CTTTGACGCT GACCGTCCTG ATGATCTGCG GACACTCTAT 
ACTTTCCAAG CTGACTTCCA GGGCAAGCTG TACCCTATCC TGGACACATG TTGGCATGAG 
AGGGGCAGCA ACTCACTGCC CATGGTATTG CCCCCACCCA GTGGGCCTGG GCACTTCACA 
CTGGGACAGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333674.1
CDS	2..1456
Translation	MAWRLTVPELQDQLQCPICLEVFKEPLMLQCGHSYCKDCLDNLSQHLDSELCCPVCRQSVDCSSSPPNVSLARVIDALRLPGDIEPTVCVHHRNPLSLFCEKDQEFICGLCGLLGSHQHHRVTPVSTVYSRMKEELAGRISELKEEHRNVEEHIGKLVNNRTRIINESDVFSWVIRREFQELHHLVDEEKARCLEGLEGHTRGLVASLDMQLEQAQGTQERLAQAEQVLEQFGNESHHEFIRKFHSVASRAEVQQARPLEGVFSPISFKPALHQADIKLTVWKRLFRKVLPAPASLKLDPATAHPLLELSKGNTVVHCGLLAQRRASQPERFDYSTCVLASKGFSWGRHYWEVVVGSKSDWRLGVIKGTASRKGKLNKSPEHGVWLIGLKEGRVYEAFGCPRLPLPVAGHPHRIGVYLHYEQGELTFFDADRPDDLRTLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGHFTLGQV

Target ORF information:

RefSeq Version	NM_001346745.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346745.1

ORF Insert Sequence:

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGAAGT TCCACTCTGT GGCCTCCAGG GCAGAGGTCC AGCAGGCTCG GCCTTTGGAA 
GGCGTCTTCA GTCCCATCTC CTTCAAGCCA GCTCTCCACC AGGCTGACAT CAAACTGACC 
GTGTGGAAGA GACTCTTCCG GAAGGTTCTG CCAGCTCCAG CATCTCTCAA GCTGGACCCG 
GCTACTGCCC ACCCTCTGCT GGAGCTCTCT AAGGGCAACA CAGTGGTGCA TTGTGGCCTC 
CTTGCCCAGA GACGAGCCAG CCAACCCGAG CGCTTCGACT ATAGCACCTG TGTCCTGGCT 
AGCAAGGGCT TCTCTTGGGG TCGCCACTAC TGGGAAGTGG TGGTGGGGAG CAAGAGTGAC 
TGGCGCTTGG GGGTCATCAA GGGCACAGCC AGCCGCAAAG GCAAGCTGAA CAAGTCCCCA 
GAGCACGGTG TGTGGCTAAT TGGCCTGAAA GAGGGTCGGG TGTATGAGGC TTTTGGCTGC 
CCACGGCTGC CACTGCCTGT GGCTGGCCAC CCTCATCGCA TCGGCGTCTA CTTACACTAT 
GAGCAGGGTG AGCTCACGTT CTTTGACGCT GACCGTCCTG ATGATCTGCG GACACTCTAT 
ACTTTCCAAG CTGACTTCCA GGGCAAGCTG TACCCTATCC TGGACACATG TTGGCATGAG 
AGGGGCAGCA ACTCACTGCC CATGGTATTG CCCCCACCCA GTGGGCCTGG GCACTTCACA 
CTGGGACAGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim50 ( XM_006504412.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504475.1 Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X2, mRNA.

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CloneID	OMu07117
Clone ID Related Accession (Same CDS sequence)	XM_006504412.3 , NM_178240.2 , NM_178240.3 , XM_006504412.4
Accession Version	XM_006504412.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504412.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGTTCC ACTCTGTGGC CTCCAGGGCA GAGGTCCAGC AGGCTCGGCC TTTGGAAGGC 
GTCTTCAGTC CCATCTCCTT CAAGCCAGCT CTCCACCAGG CTGACATCAA ACTGACCGTG 
TGGAAGAGAC TCTTCCGGAA GGTTCTGCCA GCTCCAGCAT CTCTCAAGCT GGACCCGGCT 
ACTGCCCACC CTCTGCTGGA GCTCTCTAAG GGCAACACAG TGGTGCATTG TGGCCTCCTT 
GCCCAGAGAC GAGCCAGCCA ACCCGAGCGC TTCGACTATA GCACCTGTGT CCTGGCTAGC 
AAGGGCTTCT CTTGGGGTCG CCACTACTGG GAAGTGGTGG TGGGGAGCAA GAGTGACTGG 
CGCTTGGGGG TCATCAAGGG CACAGCCAGC CGCAAAGGCA AGCTGAACAA GTCCCCAGAG 
CACGGTGTGT GGCTAATTGG CCTGAAAGAG GGTCGGGTGT ATGAGGCTTT TGGCTGCCCA 
CGGCTGCCAC TGCCTGTGGC TGGCCACCCT CATCGCATCG GCGTCTACTT ACACTATGAG 
CAGGGTGAGC TCACGTTCTT TGACGCTGAC CGTCCTGATG ATCTGCGGAC ACTCTATACT 
TTCCAAGCTG ACTTCCAGGG CAAGCTGTAC CCTATCCTGG ACACATGTTG GCATGAGAGG 
GGCAGCAACT CACTGCCCAT GGTATTGCCC CCACCCAGTG GGCCTGGGCA CTTCACACTG 
GGACAGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504475.1
CDS	203..1654
Translation	MAWRLTVPELQDQLQCPICLEVFKEPLMLQCGHSYCKDCLDNLSQHLDSELCCPVCRQSVDCSSSPPNVSLARVIDALRLPGDIEPTVCVHHRNPLSLFCEKDQEFICGLCGLLGSHQHHRVTPVSTVYSRMKEELAGRISELKEEHRNVEEHIGKLVNNRTRIINESDVFSWVIRREFQELHHLVDEEKARCLEGLEGHTRGLVASLDMQLEQAQGTQERLAQAEQVLEQFGNESHHEFIRFHSVASRAEVQQARPLEGVFSPISFKPALHQADIKLTVWKRLFRKVLPAPASLKLDPATAHPLLELSKGNTVVHCGLLAQRRASQPERFDYSTCVLASKGFSWGRHYWEVVVGSKSDWRLGVIKGTASRKGKLNKSPEHGVWLIGLKEGRVYEAFGCPRLPLPVAGHPHRIGVYLHYEQGELTFFDADRPDDLRTLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGHFTLGQV

Target ORF information:

RefSeq Version	XM_006504412.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504412.3

ORF Insert Sequence:

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGTTCC ACTCTGTGGC CTCCAGGGCA GAGGTCCAGC AGGCTCGGCC TTTGGAAGGC 
GTCTTCAGTC CCATCTCCTT CAAGCCAGCT CTCCACCAGG CTGACATCAA ACTGACCGTG 
TGGAAGAGAC TCTTCCGGAA GGTTCTGCCA GCTCCAGCAT CTCTCAAGCT GGACCCGGCT 
ACTGCCCACC CTCTGCTGGA GCTCTCTAAG GGCAACACAG TGGTGCATTG TGGCCTCCTT 
GCCCAGAGAC GAGCCAGCCA ACCCGAGCGC TTCGACTATA GCACCTGTGT CCTGGCTAGC 
AAGGGCTTCT CTTGGGGTCG CCACTACTGG GAAGTGGTGG TGGGGAGCAA GAGTGACTGG 
CGCTTGGGGG TCATCAAGGG CACAGCCAGC CGCAAAGGCA AGCTGAACAA GTCCCCAGAG 
CACGGTGTGT GGCTAATTGG CCTGAAAGAG GGTCGGGTGT ATGAGGCTTT TGGCTGCCCA 
CGGCTGCCAC TGCCTGTGGC TGGCCACCCT CATCGCATCG GCGTCTACTT ACACTATGAG 
CAGGGTGAGC TCACGTTCTT TGACGCTGAC CGTCCTGATG ATCTGCGGAC ACTCTATACT 
TTCCAAGCTG ACTTCCAGGG CAAGCTGTAC CCTATCCTGG ACACATGTTG GCATGAGAGG 
GGCAGCAACT CACTGCCCAT GGTATTGCCC CCACCCAGTG GGCCTGGGCA CTTCACACTG 
GGACAGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim50 ( XM_006504411.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504474.1 Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X1, mRNA.

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CloneID	OMu36138
Clone ID Related Accession (Same CDS sequence)	NM_001346745.1 , XM_006504411.3 , XM_006504411.4
Accession Version	XM_006504411.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504411.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGAAGT TCCACTCTGT GGCCTCCAGG GCAGAGGTCC AGCAGGCTCG GCCTTTGGAA 
GGCGTCTTCA GTCCCATCTC CTTCAAGCCA GCTCTCCACC AGGCTGACAT CAAACTGACC 
GTGTGGAAGA GACTCTTCCG GAAGGTTCTG CCAGCTCCAG CATCTCTCAA GCTGGACCCG 
GCTACTGCCC ACCCTCTGCT GGAGCTCTCT AAGGGCAACA CAGTGGTGCA TTGTGGCCTC 
CTTGCCCAGA GACGAGCCAG CCAACCCGAG CGCTTCGACT ATAGCACCTG TGTCCTGGCT 
AGCAAGGGCT TCTCTTGGGG TCGCCACTAC TGGGAAGTGG TGGTGGGGAG CAAGAGTGAC 
TGGCGCTTGG GGGTCATCAA GGGCACAGCC AGCCGCAAAG GCAAGCTGAA CAAGTCCCCA 
GAGCACGGTG TGTGGCTAAT TGGCCTGAAA GAGGGTCGGG TGTATGAGGC TTTTGGCTGC 
CCACGGCTGC CACTGCCTGT GGCTGGCCAC CCTCATCGCA TCGGCGTCTA CTTACACTAT 
GAGCAGGGTG AGCTCACGTT CTTTGACGCT GACCGTCCTG ATGATCTGCG GACACTCTAT 
ACTTTCCAAG CTGACTTCCA GGGCAAGCTG TACCCTATCC TGGACACATG TTGGCATGAG 
AGGGGCAGCA ACTCACTGCC CATGGTATTG CCCCCACCCA GTGGGCCTGG GCACTTCACA 
CTGGGACAGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504474.1
CDS	203..1657
Translation	MAWRLTVPELQDQLQCPICLEVFKEPLMLQCGHSYCKDCLDNLSQHLDSELCCPVCRQSVDCSSSPPNVSLARVIDALRLPGDIEPTVCVHHRNPLSLFCEKDQEFICGLCGLLGSHQHHRVTPVSTVYSRMKEELAGRISELKEEHRNVEEHIGKLVNNRTRIINESDVFSWVIRREFQELHHLVDEEKARCLEGLEGHTRGLVASLDMQLEQAQGTQERLAQAEQVLEQFGNESHHEFIRKFHSVASRAEVQQARPLEGVFSPISFKPALHQADIKLTVWKRLFRKVLPAPASLKLDPATAHPLLELSKGNTVVHCGLLAQRRASQPERFDYSTCVLASKGFSWGRHYWEVVVGSKSDWRLGVIKGTASRKGKLNKSPEHGVWLIGLKEGRVYEAFGCPRLPLPVAGHPHRIGVYLHYEQGELTFFDADRPDDLRTLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGHFTLGQV

Target ORF information:

RefSeq Version	XM_006504411.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504411.3

ORF Insert Sequence:

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGAAGT TCCACTCTGT GGCCTCCAGG GCAGAGGTCC AGCAGGCTCG GCCTTTGGAA 
GGCGTCTTCA GTCCCATCTC CTTCAAGCCA GCTCTCCACC AGGCTGACAT CAAACTGACC 
GTGTGGAAGA GACTCTTCCG GAAGGTTCTG CCAGCTCCAG CATCTCTCAA GCTGGACCCG 
GCTACTGCCC ACCCTCTGCT GGAGCTCTCT AAGGGCAACA CAGTGGTGCA TTGTGGCCTC 
CTTGCCCAGA GACGAGCCAG CCAACCCGAG CGCTTCGACT ATAGCACCTG TGTCCTGGCT 
AGCAAGGGCT TCTCTTGGGG TCGCCACTAC TGGGAAGTGG TGGTGGGGAG CAAGAGTGAC 
TGGCGCTTGG GGGTCATCAA GGGCACAGCC AGCCGCAAAG GCAAGCTGAA CAAGTCCCCA 
GAGCACGGTG TGTGGCTAAT TGGCCTGAAA GAGGGTCGGG TGTATGAGGC TTTTGGCTGC 
CCACGGCTGC CACTGCCTGT GGCTGGCCAC CCTCATCGCA TCGGCGTCTA CTTACACTAT 
GAGCAGGGTG AGCTCACGTT CTTTGACGCT GACCGTCCTG ATGATCTGCG GACACTCTAT 
ACTTTCCAAG CTGACTTCCA GGGCAAGCTG TACCCTATCC TGGACACATG TTGGCATGAG 
AGGGGCAGCA ACTCACTGCC CATGGTATTG CCCCCACCCA GTGGGCCTGG GCACTTCACA 
CTGGGACAGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim50 ( XM_006504412.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504475.1 Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X2, mRNA.

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CloneID	OMu07117
Clone ID Related Accession (Same CDS sequence)	XM_006504412.3 , NM_178240.2 , NM_178240.3 , XM_006504412.4
Accession Version	XM_006504412.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504412.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGTTCC ACTCTGTGGC CTCCAGGGCA GAGGTCCAGC AGGCTCGGCC TTTGGAAGGC 
GTCTTCAGTC CCATCTCCTT CAAGCCAGCT CTCCACCAGG CTGACATCAA ACTGACCGTG 
TGGAAGAGAC TCTTCCGGAA GGTTCTGCCA GCTCCAGCAT CTCTCAAGCT GGACCCGGCT 
ACTGCCCACC CTCTGCTGGA GCTCTCTAAG GGCAACACAG TGGTGCATTG TGGCCTCCTT 
GCCCAGAGAC GAGCCAGCCA ACCCGAGCGC TTCGACTATA GCACCTGTGT CCTGGCTAGC 
AAGGGCTTCT CTTGGGGTCG CCACTACTGG GAAGTGGTGG TGGGGAGCAA GAGTGACTGG 
CGCTTGGGGG TCATCAAGGG CACAGCCAGC CGCAAAGGCA AGCTGAACAA GTCCCCAGAG 
CACGGTGTGT GGCTAATTGG CCTGAAAGAG GGTCGGGTGT ATGAGGCTTT TGGCTGCCCA 
CGGCTGCCAC TGCCTGTGGC TGGCCACCCT CATCGCATCG GCGTCTACTT ACACTATGAG 
CAGGGTGAGC TCACGTTCTT TGACGCTGAC CGTCCTGATG ATCTGCGGAC ACTCTATACT 
TTCCAAGCTG ACTTCCAGGG CAAGCTGTAC CCTATCCTGG ACACATGTTG GCATGAGAGG 
GGCAGCAACT CACTGCCCAT GGTATTGCCC CCACCCAGTG GGCCTGGGCA CTTCACACTG 
GGACAGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504475.1
CDS	192..1643
Translation	MAWRLTVPELQDQLQCPICLEVFKEPLMLQCGHSYCKDCLDNLSQHLDSELCCPVCRQSVDCSSSPPNVSLARVIDALRLPGDIEPTVCVHHRNPLSLFCEKDQEFICGLCGLLGSHQHHRVTPVSTVYSRMKEELAGRISELKEEHRNVEEHIGKLVNNRTRIINESDVFSWVIRREFQELHHLVDEEKARCLEGLEGHTRGLVASLDMQLEQAQGTQERLAQAEQVLEQFGNESHHEFIRFHSVASRAEVQQARPLEGVFSPISFKPALHQADIKLTVWKRLFRKVLPAPASLKLDPATAHPLLELSKGNTVVHCGLLAQRRASQPERFDYSTCVLASKGFSWGRHYWEVVVGSKSDWRLGVIKGTASRKGKLNKSPEHGVWLIGLKEGRVYEAFGCPRLPLPVAGHPHRIGVYLHYEQGELTFFDADRPDDLRTLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGHFTLGQV

Target ORF information:

RefSeq Version	XM_006504412.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504412.4

ORF Insert Sequence:

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGTTCC ACTCTGTGGC CTCCAGGGCA GAGGTCCAGC AGGCTCGGCC TTTGGAAGGC 
GTCTTCAGTC CCATCTCCTT CAAGCCAGCT CTCCACCAGG CTGACATCAA ACTGACCGTG 
TGGAAGAGAC TCTTCCGGAA GGTTCTGCCA GCTCCAGCAT CTCTCAAGCT GGACCCGGCT 
ACTGCCCACC CTCTGCTGGA GCTCTCTAAG GGCAACACAG TGGTGCATTG TGGCCTCCTT 
GCCCAGAGAC GAGCCAGCCA ACCCGAGCGC TTCGACTATA GCACCTGTGT CCTGGCTAGC 
AAGGGCTTCT CTTGGGGTCG CCACTACTGG GAAGTGGTGG TGGGGAGCAA GAGTGACTGG 
CGCTTGGGGG TCATCAAGGG CACAGCCAGC CGCAAAGGCA AGCTGAACAA GTCCCCAGAG 
CACGGTGTGT GGCTAATTGG CCTGAAAGAG GGTCGGGTGT ATGAGGCTTT TGGCTGCCCA 
CGGCTGCCAC TGCCTGTGGC TGGCCACCCT CATCGCATCG GCGTCTACTT ACACTATGAG 
CAGGGTGAGC TCACGTTCTT TGACGCTGAC CGTCCTGATG ATCTGCGGAC ACTCTATACT 
TTCCAAGCTG ACTTCCAGGG CAAGCTGTAC CCTATCCTGG ACACATGTTG GCATGAGAGG 
GGCAGCAACT CACTGCCCAT GGTATTGCCC CCACCCAGTG GGCCTGGGCA CTTCACACTG 
GGACAGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim50 ( XM_006504411.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504474.1 Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X1, mRNA.

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CloneID	OMu36138
Clone ID Related Accession (Same CDS sequence)	NM_001346745.1 , XM_006504411.3 , XM_006504411.4
Accession Version	XM_006504411.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504411.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGAAGT TCCACTCTGT GGCCTCCAGG GCAGAGGTCC AGCAGGCTCG GCCTTTGGAA 
GGCGTCTTCA GTCCCATCTC CTTCAAGCCA GCTCTCCACC AGGCTGACAT CAAACTGACC 
GTGTGGAAGA GACTCTTCCG GAAGGTTCTG CCAGCTCCAG CATCTCTCAA GCTGGACCCG 
GCTACTGCCC ACCCTCTGCT GGAGCTCTCT AAGGGCAACA CAGTGGTGCA TTGTGGCCTC 
CTTGCCCAGA GACGAGCCAG CCAACCCGAG CGCTTCGACT ATAGCACCTG TGTCCTGGCT 
AGCAAGGGCT TCTCTTGGGG TCGCCACTAC TGGGAAGTGG TGGTGGGGAG CAAGAGTGAC 
TGGCGCTTGG GGGTCATCAA GGGCACAGCC AGCCGCAAAG GCAAGCTGAA CAAGTCCCCA 
GAGCACGGTG TGTGGCTAAT TGGCCTGAAA GAGGGTCGGG TGTATGAGGC TTTTGGCTGC 
CCACGGCTGC CACTGCCTGT GGCTGGCCAC CCTCATCGCA TCGGCGTCTA CTTACACTAT 
GAGCAGGGTG AGCTCACGTT CTTTGACGCT GACCGTCCTG ATGATCTGCG GACACTCTAT 
ACTTTCCAAG CTGACTTCCA GGGCAAGCTG TACCCTATCC TGGACACATG TTGGCATGAG 
AGGGGCAGCA ACTCACTGCC CATGGTATTG CCCCCACCCA GTGGGCCTGG GCACTTCACA 
CTGGGACAGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504474.1
CDS	192..1646
Translation	MAWRLTVPELQDQLQCPICLEVFKEPLMLQCGHSYCKDCLDNLSQHLDSELCCPVCRQSVDCSSSPPNVSLARVIDALRLPGDIEPTVCVHHRNPLSLFCEKDQEFICGLCGLLGSHQHHRVTPVSTVYSRMKEELAGRISELKEEHRNVEEHIGKLVNNRTRIINESDVFSWVIRREFQELHHLVDEEKARCLEGLEGHTRGLVASLDMQLEQAQGTQERLAQAEQVLEQFGNESHHEFIRKFHSVASRAEVQQARPLEGVFSPISFKPALHQADIKLTVWKRLFRKVLPAPASLKLDPATAHPLLELSKGNTVVHCGLLAQRRASQPERFDYSTCVLASKGFSWGRHYWEVVVGSKSDWRLGVIKGTASRKGKLNKSPEHGVWLIGLKEGRVYEAFGCPRLPLPVAGHPHRIGVYLHYEQGELTFFDADRPDDLRTLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGHFTLGQV

Target ORF information:

RefSeq Version	XM_006504411.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 50 (Trim50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504411.4

ORF Insert Sequence:

ATGGCGTGGC GGCTGACTGT GCCGGAACTC CAGGACCAGC TGCAGTGTCC CATTTGCCTG 
GAGGTCTTCA AAGAGCCTCT GATGCTACAG TGCGGCCACT CCTACTGCAA GGACTGCCTG 
GATAACTTGT CCCAACACCT GGACTCCGAG CTATGCTGTC CTGTGTGTCG TCAGTCAGTA 
GACTGCAGCA GCTCCCCGCC TAATGTCTCC CTGGCCCGGG TGATAGATGC TCTGAGGCTG 
CCGGGGGACA TAGAGCCCAC CGTCTGCGTG CACCACCGAA ACCCTCTCAG CCTCTTCTGC 
GAGAAAGACC AGGAATTCAT CTGTGGTCTC TGTGGCCTGC TGGGCTCCCA CCAACACCAC 
CGAGTCACAC CAGTCTCCAC CGTCTACAGC CGCATGAAGG AAGAGCTTGC AGGCCGCATA 
TCTGAGCTGA AGGAGGAGCA CAGGAACGTG GAGGAGCACA TCGGCAAACT GGTGAACAAC 
AGGACCCGGA TTATTAATGA GTCCGATGTC TTCAGCTGGG TGATCCGAAG GGAGTTCCAG 
GAATTGCACC ACCTGGTGGA CGAAGAGAAA GCCCGCTGCC TGGAGGGGCT TGAGGGCCAT 
ACGCGTGGCC TGGTGGCCTC CCTGGACATG CAGCTGGAGC AGGCTCAGGG TACACAAGAG 
CGACTGGCTC AGGCGGAGCA GGTGCTGGAG CAGTTTGGGA ACGAGAGTCA CCATGAGTTC 
ATCCGGAAGT TCCACTCTGT GGCCTCCAGG GCAGAGGTCC AGCAGGCTCG GCCTTTGGAA 
GGCGTCTTCA GTCCCATCTC CTTCAAGCCA GCTCTCCACC AGGCTGACAT CAAACTGACC 
GTGTGGAAGA GACTCTTCCG GAAGGTTCTG CCAGCTCCAG CATCTCTCAA GCTGGACCCG 
GCTACTGCCC ACCCTCTGCT GGAGCTCTCT AAGGGCAACA CAGTGGTGCA TTGTGGCCTC 
CTTGCCCAGA GACGAGCCAG CCAACCCGAG CGCTTCGACT ATAGCACCTG TGTCCTGGCT 
AGCAAGGGCT TCTCTTGGGG TCGCCACTAC TGGGAAGTGG TGGTGGGGAG CAAGAGTGAC 
TGGCGCTTGG GGGTCATCAA GGGCACAGCC AGCCGCAAAG GCAAGCTGAA CAAGTCCCCA 
GAGCACGGTG TGTGGCTAAT TGGCCTGAAA GAGGGTCGGG TGTATGAGGC TTTTGGCTGC 
CCACGGCTGC CACTGCCTGT GGCTGGCCAC CCTCATCGCA TCGGCGTCTA CTTACACTAT 
GAGCAGGGTG AGCTCACGTT CTTTGACGCT GACCGTCCTG ATGATCTGCG GACACTCTAT 
ACTTTCCAAG CTGACTTCCA GGGCAAGCTG TACCCTATCC TGGACACATG TTGGCATGAG 
AGGGGCAGCA ACTCACTGCC CATGGTATTG CCCCCACCCA GTGGGCCTGG GCACTTCACA 
CTGGGACAGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRIM50 protein regulates vesicular trafficking for acid secretion in gastric parietal cells.
The Journal of biological chemistry287(40)33523-32(2012 Sep)
Nishi M,Aoyama F,Kisa F,Zhu H,Sun M,Lin P,Ohta H,Van B,Yamamoto S,Kakizawa S,Sakai H,Ma J,Sawaguchi A,Takeshima H

The E3-ubiquitin ligase TRIM50 interacts with HDAC6 and p62, and promotes the sequestration and clearance of ubiquitinated proteins into the aggresome.
PloS one7(7)e40440(2012)
Fusco C,Micale L,Egorov M,Monti M,D'Addetta EV,Augello B,Cozzolino F,Calcagn? A,Fontana A,Polishchuk RS,Didelot G,Reymond A,Pucci P,Merla G

Induced chromosome deletions cause hypersociability and other features of Williams-Beuren syndrome in mice.
EMBO molecular medicine1(1)50-65(2009 Apr)
Li HH,Roy M,Kuscuoglu U,Spencer CM,Halm B,Harrison KC,Bayle JH,Splendore A,Ding F,Meltzer LA,Wright E,Paylor R,Deisseroth K,Francke U

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Trim50 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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