Vgll2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vgll2
Entrez Gene ID	215031
Full Name	vestigial like family member 2
Synonyms	C130057C21Rik,VITO-1,Vito1,vgl-2
General protein information	Preferred Names vestigial like family member 2 Names transcription cofactor vestigial-like protein 2 vestigial like 2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B3
Summary	This gene is a member of the Vestigial-like (Vgl) gene family and is upregulated during muscle differentiation. The product of this gene interacts with and modifies the DNA-binding properties of the transcription factor, TEF-1, and is important for muscle tissue development. Reduced expression of this gene leads to a reduction in the terminal differentiation of muscle cells. Alternate splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_153786.2	NP_722481.1	transcription cofactor vestigial-like protein 2 isoform 1
NM_001300957.1	NP_001287886.1	transcription cofactor vestigial-like protein 2 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	VGLL2	XP_541214.3
Gallus gallus (chicken)	VGLL2	NP_001139462.1
Mus musculus (house mouse)	Vgll2	NP_722481.1
Pan troglodytes (chimpanzee)	VGLL2	XP_001162679.1
Bos taurus (cattle)	VGLL2	NP_001071500.1
Rattus norvegicus (Norway rat)	Vgll2	NP_001101100.1
Homo sapiens (human)	VGLL2	NP_872586.1
	VGLL2	XP_001110896.1
Danio rerio (zebrafish)	si:dkeyp-93d12.1	NP_001020657.1
Xenopus tropicalis (tropical clawed frog)	vgll2	NP_989178.1

Gene Ontology
Bibliography

Related articles in PubMed

Vestigial-like 2 contributes to normal muscle fiber type distribution in mice.
Honda M, Hidaka K, Fukada SI, Sugawa R, Shirai M, Ikawa M, Morisaki T
Scientific reports7(1)7168(2017 Aug)

Arid3b is essential for second heart field cell deployment and heart patterning.
Uribe V, Bad?a-Careaga C, Casanova JC, Dom?nguez JN, de la Pompa JL, Sanz-Ezquerro JJ
Development (Cambridge, England)141(21)4168-81(2014 Nov)

Six1 and Six4 gene expression is necessary to activate the fast-type muscle gene program in the mouse primary myotome.
Niro C, Demignon J, Vincent S, Liu Y, Giordani J, Sgarioto N, Favier M, Guillet-Deniau I, Blais A, Maire P
Developmental biology338(2)168-82(2010 Feb)

VITO-2, a new SID domain protein, is expressed in the myogenic lineage during early mouse embryonic development.
Mielcarek M, Piotrowska I, Schneider A, G?nther S, Braun T
Gene expression patterns : GEP9(3)129-37(2009 Mar)

The neonatal ventromedial hypothalamus transcriptome reveals novel markers with spatially distinct patterning.
Kurrasch DM, Cheung CC, Lee FY, Tran PV, Hata K, Ingraham HA
The Journal of neuroscience : the official journal of the Society for Neuroscience27(50)13624-34(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A transcription cofactor required for skeletal muscle differentiation that modifies DNA target selectivity of the Transcriptional Enhancer Factor-1 (TEF-1) transcription factors.
Title: Transcription cofactor Vgl-2 is required for skeletal muscle differentiation.

VITO-1 is a crucial new cofactor of the muscle regulatory programme; VITO-1, a new scalloped interaction domain-containing protein, binds to TEF1 in vitro and strongly stimulates transcription of a reporter plasmid together with TEF-1
Title: VITO-1 is an essential cofactor of TEF1-dependent muscle-specific gene regulation.

The following Vgll2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vgll2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03040	NM_153786.2 Latest version!	Mus musculus vestigial like family member 2 (Vgll2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu72919	NM_001300957.1 Latest version!	Mus musculus vestigial like family member 2 (Vgll2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vgll2 ( NM_153786.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_722481.1 Mus musculus vestigial like family member 2 (Vgll2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03040
Clone ID Related Accession (Same CDS sequence)	NM_153786.2
Accession Version	NM_153786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	transcription cofactor vestigial-like protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC153950.8, AK048390.1 and AF542181.1. On Jul 17, 2014 this sequence version replaced NM_153786.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AJ578054.1, AK048390.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGTC TGGATGTTAT GTACCAGGTC TACGGTCCCC CGCAGCCTTA TTTCGCAGCC 
GCCTACACTC CCTACCACCA GAAACTAGCC TACTACTCAA AAATGCAGGA AGCTCAAGAG 
TGCGCCAGCC CTGGCAGCAG TGCCAGCGGG AGCTCCTCAT TTTCCAACCC AACCCCAGCC 
AGTGTCAAGG AAGAGGAGGG CAGCCCAGAG AAAGAGCGCC CGCCCGAAGC TGAGTACATC 
AACTCCAGAT GTGTCCTCTT CACCTACTTC CAGGGGGACA TCAGCTCTGT GGTGGACGAA 
CATTTCAGTA GGGCCCTTAG CCACCCAAGC AGCTACACCC CAAGCTGTAC CAGCAGCAAA 
GCACACAGAA GCTCTGGACC CTGGAGAGCA GAAGGCACCT TCCCGATGAG CCAGCGCAGC 
TTCCCCGCCT CCTTCTGGAA CAGCGCGTAC CAGGCGCCTG TGCCCGCGCC ACTAGGCAGT 
CCTCTGGCCG CCGCACACTC GGAGCTGCCC TTTGCCACCG ACCCCTACTC TCCCGCCACT 
CTGCACGGCC ACCTGCACCA GGGCGCGGCC GACTGGCACC ACGCGCACCC GCACCACGCG 
CACCCGCACC ATCCCTATGC GCTGGGCGGC GCCCTGGGAG CACAAGCCTC TGCCTACCCG 
CGGCCAGCAG TGCACGAGGT CTACGCGCCC CACTTCGACC CGCGCTATGG GCCGCTGCTC 
ATGCCCGCGG CCACTGGCCG CCCCGGCCGC CTGGCCCCTG CCTCGGCGCC GGCTCCCGGC 
AGCCCTCCCT GCGAGCTTGC GGCCAAGGGC GAGCCGGCGG GCAGCGCATG GGCTGCGCCC 
GGGGGACCCT TCGTGAGCCC CACGGGGGAT GTGGCCCAGA GCCTGGGTCT CAGCGTGGAC 
TCAGGTAAGC GGAGGAGGGA ATGCAGTCTC CCCTCTGCCC CTCCGGCACT GTACCCGACT 
CTGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722481.1
CDS	222..1190
Translation	MSCLDVMYQVYGPPQPYFAAAYTPYHQKLAYYSKMQEAQECASPGSSASGSSSFSNPTPASVKEEEGSPEKERPPEAEYINSRCVLFTYFQGDISSVVDEHFSRALSHPSSYTPSCTSSKAHRSSGPWRAEGTFPMSQRSFPASFWNSAYQAPVPAPLGSPLAAAHSELPFATDPYSPATLHGHLHQGAADWHHAHPHHAHPHHPYALGGALGAQASAYPRPAVHEVYAPHFDPRYGPLLMPAATGRPGRLAPASAPAPGSPPCELAAKGEPAGSAWAAPGGPFVSPTGDVAQSLGLSVDSGKRRRECSLPSAPPALYPTLG

Target ORF information:

RefSeq Version	NM_153786.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus vestigial like family member 2 (Vgll2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153786.2

ORF Insert Sequence:

ATGAGCTGTC TGGATGTTAT GTACCAGGTC TACGGTCCCC CGCAGCCTTA TTTCGCAGCC 
GCCTACACTC CCTACCACCA GAAACTAGCC TACTACTCAA AAATGCAGGA AGCTCAAGAG 
TGCGCCAGCC CTGGCAGCAG TGCCAGCGGG AGCTCCTCAT TTTCCAACCC AACCCCAGCC 
AGTGTCAAGG AAGAGGAGGG CAGCCCAGAG AAAGAGCGCC CGCCCGAAGC TGAGTACATC 
AACTCCAGAT GTGTCCTCTT CACCTACTTC CAGGGGGACA TCAGCTCTGT GGTGGACGAA 
CATTTCAGTA GGGCCCTTAG CCACCCAAGC AGCTACACCC CAAGCTGTAC CAGCAGCAAA 
GCACACAGAA GCTCTGGACC CTGGAGAGCA GAAGGCACCT TCCCGATGAG CCAGCGCAGC 
TTCCCCGCCT CCTTCTGGAA CAGCGCGTAC CAGGCGCCTG TGCCCGCGCC ACTAGGCAGT 
CCTCTGGCCG CCGCACACTC GGAGCTGCCC TTTGCCACCG ACCCCTACTC TCCCGCCACT 
CTGCACGGCC ACCTGCACCA GGGCGCGGCC GACTGGCACC ACGCGCACCC GCACCACGCG 
CACCCGCACC ATCCCTATGC GCTGGGCGGC GCCCTGGGAG CACAAGCCTC TGCCTACCCG 
CGGCCAGCAG TGCACGAGGT CTACGCGCCC CACTTCGACC CGCGCTATGG GCCGCTGCTC 
ATGCCCGCGG CCACTGGCCG CCCCGGCCGC CTGGCCCCTG CCTCGGCGCC GGCTCCCGGC 
AGCCCTCCCT GCGAGCTTGC GGCCAAGGGC GAGCCGGCGG GCAGCGCATG GGCTGCGCCC 
GGGGGACCCT TCGTGAGCCC CACGGGGGAT GTGGCCCAGA GCCTGGGTCT CAGCGTGGAC 
TCAGGTAAGC GGAGGAGGGA ATGCAGTCTC CCCTCTGCCC CTCCGGCACT GTACCCGACT 
CTGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vgll2 ( NM_001300957.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001287886.1 Mus musculus vestigial like family member 2 (Vgll2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu72919
Clone ID Related Accession (Same CDS sequence)	NM_001300957.1
Accession Version	NM_001300957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	transcription cofactor vestigial-like protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC153950.8, AK052987.1 and AF542181.1. On Jul 17, 2014 this sequence version replaced XM_006512655.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK052987.1, BB662575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGTC TGGATGTTAT GTACCAGGTC TACGGTCCCC CGCAGCCTTA TTTCGCAGCC 
GCCTACACTC CCTACCACCA GAAACTAGCC TACTACTCAA AAATGCAGGA AGCTCAAGAG 
TGCGCCAGCC CTGGCAGCAG TGCCAGCGGG AGCTCCTCAT TTTCCAACCC AACCCCAGCC 
AGTGTCAAGG AAGAGGAGGG CAGCCCAGAG AAAGAGCGCC CGCCCGAAGC TGAGTACATC 
AACTCCAGAT GTGTCCTCTT CACCTACTTC CAGGGGGACA TCAGCTCTGT GGTGGACGAA 
CATTTCAGTA GGGCCCTTAG CCACCCAAGC AGCTACACCC CAAGCTGTAC CAGCAGCAAA 
GCACACAGAA GCTCTGGACC CTGGAGAGAA GGCACCTTCC CGATGAGCCA GCGCAGCTTC 
CCCGCCTCCT TCTGGAACAG CGCGTACCAG GCGCCTGTGC CCGCGCCACT AGGCAGTCCT 
CTGGCCGCCG CACACTCGGA GCTGCCCTTT GCCACCGACC CCTACTCTCC CGCCACTCTG 
CACGGCCACC TGCACCAGGG CGCGGCCGAC TGGCACCACG CGCACCCGCA CCACGCGCAC 
CCGCACCATC CCTATGCGCT GGGCGGCGCC CTGGGAGCAC AAGCCTCTGC CTACCCGCGG 
CCAGCAGTGC ACGAGGTCTA CGCGCCCCAC TTCGACCCGC GCTATGGGCC GCTGCTCATG 
CCCGCGGCCA CTGGCCGCCC CGGCCGCCTG GCCCCTGCCT CGGCGCCGGC TCCCGGCAGC 
CCTCCCTGCG AGCTTGCGGC CAAGGGCGAG CCGGCGGGCA GCGCATGGGC TGCGCCCGGG 
GGACCCTTCG TGAGCCCCAC GGGGGATGTG GCCCAGAGCC TGGGTCTCAG CGTGGACTCA 
GGTAAGCGGA GGAGGGAATG CAGTCTCCCC TCTGCCCCTC CGGCACTGTA CCCGACTCTG 
GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287886.1
CDS	222..1187
Translation	MSCLDVMYQVYGPPQPYFAAAYTPYHQKLAYYSKMQEAQECASPGSSASGSSSFSNPTPASVKEEEGSPEKERPPEAEYINSRCVLFTYFQGDISSVVDEHFSRALSHPSSYTPSCTSSKAHRSSGPWREGTFPMSQRSFPASFWNSAYQAPVPAPLGSPLAAAHSELPFATDPYSPATLHGHLHQGAADWHHAHPHHAHPHHPYALGGALGAQASAYPRPAVHEVYAPHFDPRYGPLLMPAATGRPGRLAPASAPAPGSPPCELAAKGEPAGSAWAAPGGPFVSPTGDVAQSLGLSVDSGKRRRECSLPSAPPALYPTLG

Target ORF information:

RefSeq Version	NM_001300957.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus vestigial like family member 2 (Vgll2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300957.1

ORF Insert Sequence:

ATGAGCTGTC TGGATGTTAT GTACCAGGTC TACGGTCCCC CGCAGCCTTA TTTCGCAGCC 
GCCTACACTC CCTACCACCA GAAACTAGCC TACTACTCAA AAATGCAGGA AGCTCAAGAG 
TGCGCCAGCC CTGGCAGCAG TGCCAGCGGG AGCTCCTCAT TTTCCAACCC AACCCCAGCC 
AGTGTCAAGG AAGAGGAGGG CAGCCCAGAG AAAGAGCGCC CGCCCGAAGC TGAGTACATC 
AACTCCAGAT GTGTCCTCTT CACCTACTTC CAGGGGGACA TCAGCTCTGT GGTGGACGAA 
CATTTCAGTA GGGCCCTTAG CCACCCAAGC AGCTACACCC CAAGCTGTAC CAGCAGCAAA 
GCACACAGAA GCTCTGGACC CTGGAGAGAA GGCACCTTCC CGATGAGCCA GCGCAGCTTC 
CCCGCCTCCT TCTGGAACAG CGCGTACCAG GCGCCTGTGC CCGCGCCACT AGGCAGTCCT 
CTGGCCGCCG CACACTCGGA GCTGCCCTTT GCCACCGACC CCTACTCTCC CGCCACTCTG 
CACGGCCACC TGCACCAGGG CGCGGCCGAC TGGCACCACG CGCACCCGCA CCACGCGCAC 
CCGCACCATC CCTATGCGCT GGGCGGCGCC CTGGGAGCAC AAGCCTCTGC CTACCCGCGG 
CCAGCAGTGC ACGAGGTCTA CGCGCCCCAC TTCGACCCGC GCTATGGGCC GCTGCTCATG 
CCCGCGGCCA CTGGCCGCCC CGGCCGCCTG GCCCCTGCCT CGGCGCCGGC TCCCGGCAGC 
CCTCCCTGCG AGCTTGCGGC CAAGGGCGAG CCGGCGGGCA GCGCATGGGC TGCGCCCGGG 
GGACCCTTCG TGAGCCCCAC GGGGGATGTG GCCCAGAGCC TGGGTCTCAG CGTGGACTCA 
GGTAAGCGGA GGAGGGAATG CAGTCTCCCC TCTGCCCCTC CGGCACTGTA CCCGACTCTG 
GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vestigial-like 2 contributes to normal muscle fiber type distribution in mice.
Scientific reports7(1)7168(2017 Aug)
Honda M,Hidaka K,Fukada SI,Sugawa R,Shirai M,Ikawa M,Morisaki T

Arid3b is essential for second heart field cell deployment and heart patterning.
Development (Cambridge, England)141(21)4168-81(2014 Nov)
Uribe V,Bad?a-Careaga C,Casanova JC,Dom?nguez JN,de la Pompa JL,Sanz-Ezquerro JJ

Six1 and Six4 gene expression is necessary to activate the fast-type muscle gene program in the mouse primary myotome.
Developmental biology338(2)168-82(2010 Feb)
Niro C,Demignon J,Vincent S,Liu Y,Giordani J,Sgarioto N,Favier M,Guillet-Deniau I,Blais A,Maire P

VITO-2, a new SID domain protein, is expressed in the myogenic lineage during early mouse embryonic development.
Gene expression patterns : GEP9(3)129-37(2009 Mar)
Mielcarek M,Piotrowska I,Schneider A,G?nther S,Braun T

The neonatal ventromedial hypothalamus transcriptome reveals novel markers with spatially distinct patterning.
The Journal of neuroscience : the official journal of the Society for Neuroscience27(50)13624-34(2007 Dec)
Kurrasch DM,Cheung CC,Lee FY,Tran PV,Hata K,Ingraham HA

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Vgll2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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