Spg11 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spg11
Entrez Gene ID	214585
Full Name	SPG11, spatacsin vesicle trafficking associated
Synonyms	6030465E24Rik,A330015I11,C530005A01Rik
General protein information	Preferred Names SPG11, spatacsin vesicle trafficking associated Names spatacsin spastic paraplegia 11 protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499109.3	XP_006499172.1	spatacsin isoform X3
XM_011239422.3	XP_011237724.1	spatacsin isoform X1
XM_011239423.3	XP_011237725.1	spatacsin isoform X3
XM_011239422.2	XP_011237724.1	spatacsin isoform X1
XM_006499108.3	XP_006499171.1	spatacsin isoform X2
XM_011239423.2	XP_011237725.1	spatacsin isoform X4
NM_145531.2	NP_663506.2	spatacsin
XM_006499109.4	XP_006499172.1	spatacsin isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Spg11	NP_001178500.1
Homo sapiens (human)	SPG11	NP_079413.3
Canis lupus familiaris (dog)	SPG11	XP_003640073.2
Bos taurus (cattle)	SPG11	XP_002691138.2
Mus musculus (house mouse)	Spg11	NP_663506.2
	LOC712221	XP_002808495.1
Gallus gallus (chicken)	SPG11	XP_003641879.2
Danio rerio (zebrafish)	spg11	NP_001181923.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of spatacsin function alters lysosomal lipid clearance leading to upper and lower motor neuron degeneration.
Branchu J, Boutry M, Sourd L, Depp M, Leone C, Corriger A, Vallucci M, Esteves T, Matusiak R, Dumont M, Muriel MP, Santorelli FM, Brice A, El Hachimi KH, Stevanin G, Darios F
Neurobiology of disease10221-37(2017 Jun)

In Vivo Evidence for Lysosome Depletion and Impaired Autophagic Clearance in Hereditary Spastic Paraplegia Type SPG11.
Varga RE, Khundadze M, Damme M, Nietzsche S, Hoffmann B, Stauber T, Koch N, Hennings JC, Franzka P, Huebner AK, Kessels MM, Biskup C, Jentsch TJ, Qualmann B, Braulke T, Kurth I, Beetz C, H?bner CA
PLoS genetics11(8)e1005454(2015 Aug)

Dysfunction of spatacsin leads to axonal pathology in SPG11-linked hereditary spastic paraplegia.
P?rez-Brangul? F, Mishra HK, Prots I, Havlicek S, Kohl Z, Saul D, Rummel C, Dorca-Arevalo J, Regensburger M, Graef D, Sock E, Blasi J, Groemer TW, Schl?tzer-Schrehardt U, Winkler J, Winner B
Human molecular genetics23(18)4859-74(2014 Sep)

Cellular distribution and subcellular localization of spatacsin and spastizin, two proteins involved in hereditary spastic paraplegia.
Murmu RP, Martin E, Rastetter A, Esteves T, Muriel MP, El Hachimi KH, Denora PS, Dauphin A, Fernandez JC, Duyckaerts C, Brice A, Darios F, Stevanin G
Molecular and cellular neurosciences47(3)191-202(2011 Jul)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study provides evidence that SPG11 is implicated in axonal maintenance and cargo trafficking.
Title: Dysfunction of spatacsin leads to axonal pathology in SPG11-linked hereditary spastic paraplegia.

The following Spg11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spg11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu72893	XM_011239423.3 Latest version!	Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu30224	XM_006499109.4 Latest version!	Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu72892	XM_011239422.3 Latest version!	Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu17472	NM_145531.2 Latest version!	Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1791.30 $2559.00
OMu30223	XM_006499108.3 Latest version!	Mus musculus spastic paraplegia 11 (Spg11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu72892	XM_011239422.2	Mus musculus spastic paraplegia 11 (Spg11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu72893	XM_011239423.2	Mus musculus spastic paraplegia 11 (Spg11), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu30224	XM_006499109.3	Mus musculus spastic paraplegia 11 (Spg11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spg11 ( XM_011239423.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237725.1 Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu72893
Clone ID Related Accession (Same CDS sequence)	XM_011239423.3 , XM_011239423.2
Accession Version	XM_011239423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spatacsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239423.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTGTT GTATATGGCA GAAACTCAGC AGAGGAACAG AGTGGAGCAA GCCTATTCCA 
TCTCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237725.1
CDS	1067..6955
Translation	MAAQPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCCLPGPFRQFLWEESQNSTSRDRPKLLVAREDYELLVYEFDLRDGRCDAALLHGCCGRTLQKLTEDQGVSISFKSLRILSFHNNTSLLLINRCLILRVVFPGKEPGVPVLDCLSLPLPAQAADMIIDAQLCERFLFVLSTVGLIYIFNTMDGTQVAQVDLALLREDSEQLEPASVSSFTSLRVSQDLDVLVLVSSASTAVALNLHLYFRQHPGHLLCEGTLEDLPIEGPPGIDEDDLVNSAHNMKLSKVSFQVDRSWRAQLLSLNESVRGSEPEVSCCAPWFQSALRLESLESADHTPTVPTHVFIPGDVPRGRCAFPQKEHVKSSDPGRPWKTMHLSEHEQPTELTCLSVTGFTALFTWAVGATSCTIGLWDLETQSMQCFSLSQKCTPVDIGGDQQLCLALTDDGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSQSPAPDQQHPFSSHLYLRQVEEMSPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQIRELCVHTEELDEHFQKGVAILTSYINELRTFMIKFPWKPGDAIDESDVNEGVVTVKEDRVWEELSFEEVIADAILNNRIPEAQTFFRISGHSAQRLEELVRIGLDLAFDSLKKNNVEEASRLLRNMGFSVEDELLKICFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQLEALYSGHFQENAQTQAFPRYWIKEQDCFKHKSVLDTFLKYDKKDEFHKQDHRIALNWAHRWDLQTQECILLRRLSPEEYKARSPEALWRHLTARHDCSSISLWMEEFQTQETESPQQSKWPPLSADIIEQGTCCHSHMRNEILDKLARSGIFLASELEDFERLLLRLSRIGGVMQDSLPVQSYKSRAGCDFHSRFILYCLEHGLQHLLYTYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQNEDSENCLKKVDPQLLKVALTPYPKLKAALFPQYTAPSILPSDITLYHLIQSLPPFDPSRLFVWQSANTLAIGDTGSQLPHFSSPDLVSKYAVLERLNYAYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFTNPSVGAACVCFLELLGLSSLKLRVDLKMANVILGSKRRDEDARSSFIRESLAEKLSKLADGDRAATEELLVLLEEGVWDSIEQQGFSRLSSESSSQWALVLQFCMLHDRKLSVSYLRECAKANDWLQFLVHSQLHNYHPAEVESLLQYFSPVLQSHLKLASEKLSSGSISRDDSCLQELQKNKGETSNFFEILHRCSDESTSWSWLLAEAVRHRAPILSVLASCVQGASVVSCLCVWIVTSVEDKVAAEAMGHIQISVEDHHWSLKDLSIIWRTVLTRRKSHTLIRGFQLFIKDSPLLLIMEMYELCMFFKNYEKAKVKLLEFQKSLETLDTVAARVLPIIPASWMKDQVCFLLKLMPQQCETQYELGKLLQLFVGTEQLFSDGPDVQKLCLLSQVLKDTPIAISPAVISSYSTENFQRECRSILEKLKADGQFAVARRVAELAALPVDSLLIEQLTQEMQTLTHTPQWSLKQERLGFWKKCHEIFKKNSISKRAASSFFSSQAPEVSEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEGLEKQIWICRVAQHTCGGAEEEAKPSLSQHKLAAAELSFDSLASELSFSKLAALNTSKYLGLNDLPSKTTCENRLKHKEQESLNTLIGQLLDGGCVHEASRVCQYFRFYSQDLVLVLHCRALASAEATMEDLHSEIRALLSSAAQPEDLESPSVPLRKAHSCCIWQKLSRGTEWSKPIPSP

Target ORF information:

RefSeq Version	XM_011239423.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239423.3

ORF Insert Sequence:

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTGTT GTATATGGCA GAAACTCAGC AGAGGAACAG AGTGGAGCAA GCCTATTCCA 
TCTCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg11 ( XM_011239423.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237725.1 Mus musculus spastic paraplegia 11 (Spg11), transcript variant X5, mRNA.

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CloneID	OMu72893
Clone ID Related Accession (Same CDS sequence)	XM_011239423.3 , XM_011239423.2
Accession Version	XM_011239423.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spatacsin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTGTT GTATATGGCA GAAACTCAGC AGAGGAACAG AGTGGAGCAA GCCTATTCCA 
TCTCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237725.1
CDS	406..6294
Translation	MAAQPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCCLPGPFRQFLWEESQNSTSRDRPKLLVAREDYELLVYEFDLRDGRCDAALLHGCCGRTLQKLTEDQGVSISFKSLRILSFHNNTSLLLINRCLILRVVFPGKEPGVPVLDCLSLPLPAQAADMIIDAQLCERFLFVLSTVGLIYIFNTMDGTQVAQVDLALLREDSEQLEPASVSSFTSLRVSQDLDVLVLVSSASTAVALNLHLYFRQHPGHLLCEGTLEDLPIEGPPGIDEDDLVNSAHNMKLSKVSFQVDRSWRAQLLSLNESVRGSEPEVSCCAPWFQSALRLESLESADHTPTVPTHVFIPGDVPRGRCAFPQKEHVKSSDPGRPWKTMHLSEHEQPTELTCLSVTGFTALFTWAVGATSCTIGLWDLETQSMQCFSLSQKCTPVDIGGDQQLCLALTDDGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSQSPAPDQQHPFSSHLYLRQVEEMSPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQIRELCVHTEELDEHFQKGVAILTSYINELRTFMIKFPWKPGDAIDESDVNEGVVTVKEDRVWEELSFEEVIADAILNNRIPEAQTFFRISGHSAQRLEELVRIGLDLAFDSLKKNNVEEASRLLRNMGFSVEDELLKICFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQLEALYSGHFQENAQTQAFPRYWIKEQDCFKHKSVLDTFLKYDKKDEFHKQDHRIALNWAHRWDLQTQECILLRRLSPEEYKARSPEALWRHLTARHDCSSISLWMEEFQTQETESPQQSKWPPLSADIIEQGTCCHSHMRNEILDKLARSGIFLASELEDFERLLLRLSRIGGVMQDSLPVQSYKSRAGCDFHSRFILYCLEHGLQHLLYTYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQNEDSENCLKKVDPQLLKVALTPYPKLKAALFPQYTAPSILPSDITLYHLIQSLPPFDPSRLFVWQSANTLAIGDTGSQLPHFSSPDLVSKYAVLERLNYAYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFTNPSVGAACVCFLELLGLSSLKLRVDLKMANVILGSKRRDEDARSSFIRESLAEKLSKLADGDRAATEELLVLLEEGVWDSIEQQGFSRLSSESSSQWALVLQFCMLHDRKLSVSYLRECAKANDWLQFLVHSQLHNYHPAEVESLLQYFSPVLQSHLKLASEKLSSGSISRDDSCLQELQKNKGETSNFFEILHRCSDESTSWSWLLAEAVRHRAPILSVLASCVQGASVVSCLCVWIVTSVEDKVAAEAMGHIQISVEDHHWSLKDLSIIWRTVLTRRKSHTLIRGFQLFIKDSPLLLIMEMYELCMFFKNYEKAKVKLLEFQKSLETLDTVAARVLPIIPASWMKDQVCFLLKLMPQQCETQYELGKLLQLFVGTEQLFSDGPDVQKLCLLSQVLKDTPIAISPAVISSYSTENFQRECRSILEKLKADGQFAVARRVAELAALPVDSLLIEQLTQEMQTLTHTPQWSLKQERLGFWKKCHEIFKKNSISKRAASSFFSSQAPEVSEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEGLEKQIWICRVAQHTCGGAEEEAKPSLSQHKLAAAELSFDSLASELSFSKLAALNTSKYLGLNDLPSKTTCENRLKHKEQESLNTLIGQLLDGGCVHEASRVCQYFRFYSQDLVLVLHCRALASAEATMEDLHSEIRALLSSAAQPEDLESPSVPLRKAHSCCIWQKLSRGTEWSKPIPSP

Target ORF information:

RefSeq Version	XM_011239423.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 11 (Spg11), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239423.2

ORF Insert Sequence:

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTGTT GTATATGGCA GAAACTCAGC AGAGGAACAG AGTGGAGCAA GCCTATTCCA 
TCTCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg11 ( XM_006499109.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499172.1 Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), transcript variant X3, mRNA.

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CloneID	OMu30224
Clone ID Related Accession (Same CDS sequence)	XM_006499109.3 , XM_006499109.4
Accession Version	XM_006499109.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499109.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499172.1
CDS	1067..7318
Translation	MAAQPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCCLPGPFRQFLWEESQNSTSRDRPKLLVAREDYELLVYEFDLRDGRCDAALLHGCCGRTLQKLTEDQGVSISFKSLRILSFHNNTSLLLINRCLILRVVFPGKEPGVPVLDCLSLPLPAQAADMIIDAQLCERFLFVLSTVGLIYIFNTMDGTQVAQVDLALLREDSEQLEPASVSSFTSLRVSQDLDVLVLVSSASTAVALNLHLYFRQHPGHLLCEGTLEDLPIEGPPGIDEDDLVNSAHNMKLSKVSFQVDRSWRAQLLSLNESVRGSEPEVSCCAPWFQSALRLESLESADHTPTVPTHVFIPGDVPRGRCAFPQKEHVKSSDPGRPWKTMHLSEHEQPTELTCLSVTGFTALFTWAVGATSCTIGLWDLETQSMQCFSLSQKCTPVDIGGDQQLCLALTDDGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSQSPAPDQQHPFSSHLYLRQVEEMSPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQIRELCVHTEELDEHFQKGVAILTSYINELRTFMIKFPWKPGDAIDESDVNEGVVTVKEDRVWEELSFEEVIADAILNNRIPEAQTFFRISGHSAQRLEELVRIGLDLAFDSLKKNNVEEASRLLRNMGFSVEDELLKICFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQLEALYSGHFQENAQTQAFPRYWIKEQDCFKHKSVLDTFLKYDKKDEFHKQDHRIALNWAHRWDLQTQECILLRRLSPEEYKARSPEALWRHLTARHDCSSISLWMEEFQTQETESPQQSKWPPLSADIIEQGTCCHSHMRNEILDKLARSGIFLASELEDFERLLLRLSRIGGVMQDSLPVQSYKSRAGCDFHSRFILYCLEHGLQHLLYTYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQNEDSENCLKKVDPQLLKVALTPYPKLKAALFPQYTAPSILPSDITLYHLIQSLPPFDPSRLFVWQSANTLAIGDTGSQLPHFSSPDLVSKYAVLERLNYAYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFTNPSVGAACVCFLELLGLSSLKLRVDLKMANVILGSKRRDEDARSSFIRESLAEKLSKLADGDRAATEELLVLLEEGVWDSIEQQGFSRLSSESSSQWALVLQFCMLHDRKLSVSYLRECAKANDWLQFLVHSQLHNYHPAEVESLLQYFSPVLQSHLKLASEKLSSGSISRDDSCLQELQKNKGETSNFFEILHRCSDESTSWSWLLAEAVRHRAPILSVLASCVQGASVVSCLCVWIVTSVEDKVAAEAMGHIQISVEDHHWSLKDLSIIWRTVLTRRKSHTLIRGFQLFIKDSPLLLIMEMYELCMFFKNYEKAKVKLLEFQKSLETLDTVAARVLPIIPASWMKDQVCFLLKLMPQQCETQYELGKLLQLFVGTEQLFSDGPDVQKLCLLSQVLKDTPIAISPAVISSYSTENFQRECRSILEKLKADGQFAVARRVAELAALPVDSLLIEQLTQEMQTLTHTPQWSLKQERLGFWKKCHEIFKKNSISKRAASSFFSSQAPEVSEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEGLEKQIWICRVAQHTCGGAEEEAKPSLSQHKLAAAELSFDSLASELSFSKLAALNTSKYLGLNDLPSKTTCENRLKHKEQESLNTLIGQLLDGGCVHEASRVCQYFRFYSQDLVLVLHCRALASAEATMEDLHSEIRALLSSAAQPEDLESPSVPLRKAHSSSSLDSQSFVMVPPTDEVAQNLHTLISKCLHGKNYCRQVLCLYELAKDLGCSYGDVAARDSEAMLRAILASQRPDRCRQAQVFINTQGLEADTVAELVAEEVTRELLTPSEGTGEKQPFNPAEESQTFLQLTALCQDRTLF

Target ORF information:

RefSeq Version	XM_006499109.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499109.4

ORF Insert Sequence:

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg11 ( XM_011239422.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237724.1 Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), transcript variant X1, mRNA.

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CloneID	OMu72892
Clone ID Related Accession (Same CDS sequence)	XM_011239422.3 , XM_011239422.2
Accession Version	XM_011239422.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGGTAG GCATGAAGTT GTTGGACAAG ATTCCCTCTG TCCCCCACGG GGAGCTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAC CACTGCTTTA CCTTCACGTG CCACATGGAA 
GGCATCACCA GAGTCCTGCA AGCCGCCCGC ATGCTCACAG ATAACCACTT GGCTCCCAAT 
GAGGAGTACG GATTAGTGGT CCGCCTCCTC ACTGGCATCG GGCGCTACAA TGAGATGACG 
TACATATTTG ACTTGCTGCA TCAAAAGCAT TATTTCGAAG TTCTGATGAG AAAGAAGCTA 
GACCCAGTAG GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237724.1
CDS	1067..7624
Translation	MAAQPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCCLPGPFRQFLWEESQNSTSRDRPKLLVAREDYELLVYEFDLRDGRCDAALLHGCCGRTLQKLTEDQGVSISFKSLRILSFHNNTSLLLINRCLILRVVFPGKEPGVPVLDCLSLPLPAQAADMIIDAQLCERFLFVLSTVGLIYIFNTMDGTQVAQVDLALLREDSEQLEPASVSSFTSLRVSQDLDVLVLVSSASTAVALNLHLYFRQHPGHLLCEGTLEDLPIEGPPGIDEDDLVNSAHNMKLSKVSFQVDRSWRAQLLSLNESVRGSEPEVSCCAPWFQSALRLESLESADHTPTVPTHVFIPGDVPRGRCAFPQKEHVKSSDPGRPWKTMHLSEHEQPTELTCLSVTGFTALFTWAVGATSCTIGLWDLETQSMQCFSLSQKCTPVDIGGDQQLCLALTDDGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSQSPAPDQQHPFSSHLYLRQVEEMSPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQIRELCVHTEELDEHFQKGVAILTSYINELRTFMIKFPWKPGDAIDESDVNEGVVTVKEDRVWEELSFEEVIADAILNNRIPEAQTFFRISGHSAQRLEELVRIGLDLAFDSLKKNNVEEASRLLRNMGFSVEDELLKICFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQLEALYSGHFQENAQTQAFPRYWIKEQDCFKHKSVLDTFLKYDKKDEFHKQDHRIALNWAHRWDLQTQECILLRRLSPEEYKARSPEALWRHLTARHDCSSISLWMEEFQTQETESPQQSKWPPLSADIIEQGTCCHSHMRNEILDKLARSGIFLASELEDFERLLLRLSRIGGVMQDSLPVQSYKSRAGCDFHSRFILYCLEHGLQHLLYTYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQNEDSENCLKKVDPQLLKVALTPYPKLKAALFPQYTAPSILPSDITLYHLIQSLPPFDPSRLFVWQSANTLAIGDTGSQLPHFSSPDLVSKYAVLERLNYAYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFTNPSVGAACVCFLELLGLSSLKLRVDLKMANVILGSKRRDEDARSSFIRESLAEKLSKLADGDRAATEELLVLLEEGVWDSIEQQGFSRLSSESSSQWALVLQFCMLHDRKLSVSYLRECAKANDWLQFLVHSQLHNYHPAEVESLLQYFSPVLQSHLKLASEKLSSGSISRDDSCLQELQKNKGETSNFFEILHRCSDESTSWSWLLAEAVRHRAPILSVLASCVQGASVVSCLCVWIVTSVEDKVAAEAMGHIQISVEDHHWSLKDLSIIWRTVLTRRKSHTLIRGFQLFIKDSPLLLIMEMYELCMFFKNYEKAKVKLLEFQKSLETLDTVAARVLPIIPASWMKDQVCFLLKLMPQQCETQYELGKLLQLFVGTEQLFSDGPDVQKLCLLSQVLKDTPIAISPAVISSYSTENFQRECRSILEKLKADGQFAVARRVAELAALPVDSLLIEQLTQEMQTLTHTPQWSLKQERLGFWKKCHEIFKKNSISKRAASSFFSSQAPEVSEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEGLEKQIWICRVAQHTCGGAEEEAKPSLSQHKLAAAELSFDSLASELSFSKLAALNTSKYLGLNDLPSKTTCENRLKHKEQESLNTLIGQLLDGGCVHEASRVCQYFRFYSQDLVLVLHCRALASAEATMEDLHSEIRALLSSAAQPEDLESPSVPLRKAHSSSSLDSQSFVMVPPTDEVAQNLHTLISKCLHGKNYCRQVLCLYELAKDLGCSYGDVAARDSEAMLRAILASQRPDRCRQAQVFINTQGLEADTVAELVAEEVTRELLTPSEGTGEKQPFNPAEESQTFLQLTALCQDRTLVGMKLLDKIPSVPHGELSCTTELLILAHHCFTFTCHMEGITRVLQAARMLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHQKHYFEVLMRKKLDPVGV

Target ORF information:

RefSeq Version	XM_011239422.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239422.3

ORF Insert Sequence:

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGGTAG GCATGAAGTT GTTGGACAAG ATTCCCTCTG TCCCCCACGG GGAGCTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAC CACTGCTTTA CCTTCACGTG CCACATGGAA 
GGCATCACCA GAGTCCTGCA AGCCGCCCGC ATGCTCACAG ATAACCACTT GGCTCCCAAT 
GAGGAGTACG GATTAGTGGT CCGCCTCCTC ACTGGCATCG GGCGCTACAA TGAGATGACG 
TACATATTTG ACTTGCTGCA TCAAAAGCAT TATTTCGAAG TTCTGATGAG AAAGAAGCTA 
GACCCAGTAG GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg11 ( NM_145531.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663506.2 Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), mRNA.

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CloneID	OMu17472
Clone ID Related Accession (Same CDS sequence)	NM_145531.2
Accession Version	NM_145531.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	spatacsin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845457.16. On or before Apr 19, 2009 this sequence version replaced XM_904251.3, NM_145531.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK147500.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGGTAG GCATGAAGTT GTTGGACAAG ATTCCCTCTG TCCCCCACGG GGAGCTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAC CACTGCTTTA CCTTCACGTG CCACATGGAA 
GGCATCACCA GAGTCCTGCA AGCCGCCCGC ATGCTCACAG ATAACCACTT GGCTCCCAAT 
GAGGAGTACG GATTAGTGGT CCGCCTCCTC ACTGGCATCG GGCGCTACAA TGAGATGACG 
TACATATTTG ACTTGCTGCA TCAAAAGCAT TATTTCGAAG TTCTGATGAG AAAGAAGCTA 
GACCCAACCG GCACCCTGAA GACAGCCCTA CTAGACTACA TCAAACGTTG CCGCCCTGGA 
GACAGCGAAA AGCACAATAT GATTGCCCTG TGCTTCAGTA TGTGCCGGGA GATAGGAGAG 
AATCACGAGG CAGCAGCCTG CATCCAACTG AAGCTGATTG AGTCTCAGCC CTGGGAGGAA 
AGCCTCAAGG ATGGAGCCCA ACTAAAACAG CTCCTGCTTA AAGCCCTGAC TCTGATGTTG 
GATGCAGCAG AGAGTTACGC CAAGGACTCC TGTGTTCGAC AGGCCCTGCA CTGTAACCGG 
CTCACCAAGT TGATAACACT ACAGATTCAC TTTCTCAACA GTGGCCAGAA TACAATGCTT 
ATCAACTTGG GCCATCAGAA GCTAATGGAC TGTATCATGA CCCTACCTCG GTTCTACCAG 
GCTTCTATTG TAGCTGAGGC CTATGACTTT GTTCCTGACT GGGCTGAAGT ACTCTACCAA 
CAAGTGATTC TTAAAGGAGA CTTTAGTTAT TTGGAAGAAT TTAAGCAGCA GAAGTTACTA 
AGACCCAATA TATTTGAAGA CATTTCCAAA AAATATAAAC AACACCAGCC TACCGACAGG 
GTCACAGAAA ACCTGAAGAA GTTACTTTCA TATTGTGAAG ATATCTACCT GTATTACAAG 
TTGGCATATG AACACAAATT TTTTGAAATT GTAAATATGC TTCTGAAGGA CCCTCAGACG 
GGATGCTGCC TTAAAGATAT GCTCGCAGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663506.2
CDS	19..7311
Translation	MAAQPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCCLPGPFRQFLWEESQNSTSRDRPKLLVAREDYELLVYEFDLRDGRCDAALLHGCCGRTLQKLTEDQGVSISFKSLRILSFHNNTSLLLINRCLILRVVFPGKEPGVPVLDCLSLPLPAQAADMIIDAQLCERFLFVLSTVGLIYIFNTMDGTQVAQVDLALLREDSEQLEPASVSSFTSLRVSQDLDVLVLVSSASTAVALNLHLYFRQHPGHLLCEGTLEDLPIEGPPGIDEDDLVNSAHNMKLSKVSFQVDRSWRAQLLSLNESVRGSEPEVSCCAPWFQSALRLESLESADHTPTVPTHVFIPGDVPRGRCAFPQKEHVKSSDPGRPWKTMHLSEHEQPTELTCLSVTGFTALFTWAVGATSCTIGLWDLETQSMQCFSLSQKCTPVDIGGDQQLCLALTDDGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSQSPAPDQQHPFSSHLYLRQVEEMSPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQIRELCVHTEELDEHFQKGVAILTSYINELRTFMIKFPWKPGDAIDESDVNEGVVTVKEDRVWEELSFEEVIADAILNNRIPEAQTFFRISGHSAQRLEELVRIGLDLAFDSLKKNNVEEASRLLRNMGFSVEDELLKICFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQLEALYSGHFQENAQTQAFPRYWIKEQDCFKHKSVLDTFLKYDKKDEFHKQDHRIALNWAHRWDLQTQECILLRRLSPEEYKARSPEALWRHLTARHDCSSISLWMEEFQTQETESPQQSKWPPLSADIIEQGTCCHSHMRNEILDKLARSGIFLASELEDFERLLLRLSRIGGVMQDSLPVQSYKSRAGCDFHSRFILYCLEHGLQHLLYTYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQNEDSENCLKKVDPQLLKVALTPYPKLKAALFPQYTAPSILPSDITLYHLIQSLPPFDPSRLFVWQSANTLAIGDTGSQLPHFSSPDLVSKYAVLERLNYAYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFTNPSVGAACVCFLELLGLSSLKLRVDLKMANVILGSKRRDEDARSSFIRESLAEKLSKLADGDRAATEELLVLLEEGVWDSIEQQGFSRLSSESSSQWALVLQFCMLHDRKLSVSYLRECAKANDWLQFLVHSQLHNYHPAEVESLLQYFSPVLQSHLKLASEKLSSGSISRDDSCLQELQKNKGETSNFFEILHRCSDESTSWSWLLAEAVRHRAPILSVLASCVQGASVVSCLCVWIVTSVEDKVAAEAMGHIQISVEDHHWSLKDLSIIWRTVLTRRKSHTLIRGFQLFIKDSPLLLIMEMYELCMFFKNYEKAKVKLLEFQKSLETLDTVAARVLPIIPASWMKDQVCFLLKLMPQQCETQYELGKLLQLFVGTEQLFSDGPDVQKLCLLSQVLKDTPIAISPAVISSYSTENFQRECRSILEKLKADGQFAVARRVAELAALPVDSLLIEQLTQEMQTLTHTPQWSLKQERLGFWKKCHEIFKKNSISKRAASSFFSSQAPEVSEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEGLEKQIWICRVAQHTCGGAEEEAKPSLSQHKLAAAELSFDSLASELSFSKLAALNTSKYLGLNDLPSKTTCENRLKHKEQESLNTLIGQLLDGGCVHEASRVCQYFRFYSQDLVLVLHCRALASAEATMEDLHSEIRALLSSAAQPEDLESPSVPLRKAHSSSSLDSQSFVMVPPTDEVAQNLHTLISKCLHGKNYCRQVLCLYELAKDLGCSYGDVAARDSEAMLRAILASQRPDRCRQAQVFINTQGLEADTVAELVAEEVTRELLTPSEGTGEKQPFNPAEESQTFLQLTALCQDRTLVGMKLLDKIPSVPHGELSCTTELLILAHHCFTFTCHMEGITRVLQAARMLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHQKHYFEVLMRKKLDPTGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLKDGAQLKQLLLKALTLMLDAAESYAKDSCVRQALHCNRLTKLITLQIHFLNSGQNTMLINLGHQKLMDCIMTLPRFYQASIVAEAYDFVPDWAEVLYQQVILKGDFSYLEEFKQQKLLRPNIFEDISKKYKQHQPTDRVTENLKKLLSYCEDIYLYYKLAYEHKFFEIVNMLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	NM_145531.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPG11, spatacsin vesicle trafficking associated (Spg11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145531.2

ORF Insert Sequence:

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGGTAG GCATGAAGTT GTTGGACAAG ATTCCCTCTG TCCCCCACGG GGAGCTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAC CACTGCTTTA CCTTCACGTG CCACATGGAA 
GGCATCACCA GAGTCCTGCA AGCCGCCCGC ATGCTCACAG ATAACCACTT GGCTCCCAAT 
GAGGAGTACG GATTAGTGGT CCGCCTCCTC ACTGGCATCG GGCGCTACAA TGAGATGACG 
TACATATTTG ACTTGCTGCA TCAAAAGCAT TATTTCGAAG TTCTGATGAG AAAGAAGCTA 
GACCCAACCG GCACCCTGAA GACAGCCCTA CTAGACTACA TCAAACGTTG CCGCCCTGGA 
GACAGCGAAA AGCACAATAT GATTGCCCTG TGCTTCAGTA TGTGCCGGGA GATAGGAGAG 
AATCACGAGG CAGCAGCCTG CATCCAACTG AAGCTGATTG AGTCTCAGCC CTGGGAGGAA 
AGCCTCAAGG ATGGAGCCCA ACTAAAACAG CTCCTGCTTA AAGCCCTGAC TCTGATGTTG 
GATGCAGCAG AGAGTTACGC CAAGGACTCC TGTGTTCGAC AGGCCCTGCA CTGTAACCGG 
CTCACCAAGT TGATAACACT ACAGATTCAC TTTCTCAACA GTGGCCAGAA TACAATGCTT 
ATCAACTTGG GCCATCAGAA GCTAATGGAC TGTATCATGA CCCTACCTCG GTTCTACCAG 
GCTTCTATTG TAGCTGAGGC CTATGACTTT GTTCCTGACT GGGCTGAAGT ACTCTACCAA 
CAAGTGATTC TTAAAGGAGA CTTTAGTTAT TTGGAAGAAT TTAAGCAGCA GAAGTTACTA 
AGACCCAATA TATTTGAAGA CATTTCCAAA AAATATAAAC AACACCAGCC TACCGACAGG 
GTCACAGAAA ACCTGAAGAA GTTACTTTCA TATTGTGAAG ATATCTACCT GTATTACAAG 
TTGGCATATG AACACAAATT TTTTGAAATT GTAAATATGC TTCTGAAGGA CCCTCAGACG 
GGATGCTGCC TTAAAGATAT GCTCGCAGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg11 ( XM_006499108.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499171.1 Mus musculus spastic paraplegia 11 (Spg11), transcript variant X2, mRNA.

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CloneID	OMu30223
Clone ID Related Accession (Same CDS sequence)	XM_006499108.3
Accession Version	XM_006499108.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499108.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGCCAC AGAGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499171.1
CDS	406..6666
Translation	MAAQPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCCLPGPFRQFLWEESQNSTSRDRPKLLVAREDYELLVYEFDLRDGRCDAALLHGCCGRTLQKLTEDQGVSISFKSLRILSFHNNTSLLLINRCLILRVVFPGKEPGVPVLDCLSLPLPAQAADMIIDAQLCERFLFVLSTVGLIYIFNTMDGTQVAQVDLALLREDSEQLEPASVSSFTSLRVSQDLDVLVLVSSASTAVALNLHLYFRQHPGHLLCEGTLEDLPIEGPPGIDEDDLVNSAHNMKLSKVSFQVDRSWRAQLLSLNESVRGSEPEVSCCAPWFQSALRLESLESADHTPTVPTHVFIPGDVPRGRCAFPQKEHVKSSDPGRPWKTMHLSEHEQPTELTCLSVTGFTALFTWAVGATSCTIGLWDLETQSMQCFSLSQKCTPVDIGGDQQLCLALTDDGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSQSPAPDQQHPFSSHLYLRQVEEMSPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQIRELCVHTEELDEHFQKGVAILTSYINELRTFMIKFPWKPGDAIDESDVNEGVVTVKEDRVWEELSFEEVIADAILNNRIPEAQTFFRISGHSAQRLEELVRIGLDLAFDSLKKNNVEEASRLLRNMGFSVEDELLKICFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQLEALYSGHFQENAQTQAFPRYWIKEQDCFKHKSVLDTFLKYDKKDEFHKQDHRIALNWAHRWDLQTQECILLRRLSPEEYKARSPEALWRHLTARHDCSSISLWMEEFQTQETESPQQSKWPPLSADIIEQGTCCHSHMRNEILDKLARSGIFLASELEDFERLLLRLSRIGGVMQDSLPVQSYKSRAGCDFHSRFILYCLEHGLQHLLYTYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQNEDSENCLKKVDPQLLKVALTPYPKLKAALFPQYTAPSILPSDITLYHLIQSLPPFDPSRLFVWQSANTLAIGDTGSQLPHFSSPDLVSKYAVLERLNYAYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFTNPSVGAACVCFLELLGLSSLKLRVDLKMANVILGSKRRDEDARSSFIRESLAEKLSKLADGDRAATEELLVLLEEGVWDSIEQQGFSRLSSESSSQWALVLQFCMLHDRKLSVSYLRECAKANDWLQFLVHSQLHNYHPAEVESLLQYFSPVLQSHLKLASEKLSSGSISRDDSCLQELQKNKGETSNFFEILHRCSDESTSWSWLLAEAVRHRAPILSVLASCVQGASVVSCLCVWIVTSVEDKVAAEAMGHIQISVEDHHWSLKDLSIIWRTVLTRRKSHTLIRGFQLFIKDSPLLLIMEMYELCMFFKNYEKAKVKLLEFQKSLETLDTVAARVLPIIPASWMKDQVCFLLKLMPQQCETQYELGKLLQLFVGTEQLFSDGPDVQKLCLLSQVLKDTPIAISPAVISSYSTENFQRECRSILEKLKADGQFAVARRVAELAALPVDSLLIEQLTQEMQTLTHTPQWSLKQERLGFWKKCHEIFKKNSISKRAASSFFSSQAPEVSEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEGLEKQIWICRVAQHTCGGAEEEAKPSLSQHKLAAAELSFDSLASELSFSKLAALNTSKYLGLNDLPSKTTCENRLKHKEQESLNTLIGQLLDGGCVHEASRVCQYFRFYSQDLVLVLHCRALASAEATMEDLHSEIRALLSSAAQPEDLESPSVPLRKAHSSSSLDSQSFVMVPPTDEVAQNLHTLISKCLHGKNYCRQVLCLYELAKDLGCSYGDVAARDSEAMLRAILASQRPDRCRQAQVFINTQGLEADTVAELVAEEVTRELLTPSEGTGEKQPFNPAEESQTFLQLTALCQDRTLPQSS

Target ORF information:

RefSeq Version	XM_006499108.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 11 (Spg11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499108.3

ORF Insert Sequence:

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGCCAC AGAGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg11 ( XM_006499109.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499172.1 Mus musculus spastic paraplegia 11 (Spg11), transcript variant X3, mRNA.

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CloneID	OMu30224
Clone ID Related Accession (Same CDS sequence)	XM_006499109.3 , XM_006499109.4
Accession Version	XM_006499109.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spatacsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499109.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499172.1
CDS	406..6657
Translation	MAAQPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCCLPGPFRQFLWEESQNSTSRDRPKLLVAREDYELLVYEFDLRDGRCDAALLHGCCGRTLQKLTEDQGVSISFKSLRILSFHNNTSLLLINRCLILRVVFPGKEPGVPVLDCLSLPLPAQAADMIIDAQLCERFLFVLSTVGLIYIFNTMDGTQVAQVDLALLREDSEQLEPASVSSFTSLRVSQDLDVLVLVSSASTAVALNLHLYFRQHPGHLLCEGTLEDLPIEGPPGIDEDDLVNSAHNMKLSKVSFQVDRSWRAQLLSLNESVRGSEPEVSCCAPWFQSALRLESLESADHTPTVPTHVFIPGDVPRGRCAFPQKEHVKSSDPGRPWKTMHLSEHEQPTELTCLSVTGFTALFTWAVGATSCTIGLWDLETQSMQCFSLSQKCTPVDIGGDQQLCLALTDDGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSQSPAPDQQHPFSSHLYLRQVEEMSPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQIRELCVHTEELDEHFQKGVAILTSYINELRTFMIKFPWKPGDAIDESDVNEGVVTVKEDRVWEELSFEEVIADAILNNRIPEAQTFFRISGHSAQRLEELVRIGLDLAFDSLKKNNVEEASRLLRNMGFSVEDELLKICFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQLEALYSGHFQENAQTQAFPRYWIKEQDCFKHKSVLDTFLKYDKKDEFHKQDHRIALNWAHRWDLQTQECILLRRLSPEEYKARSPEALWRHLTARHDCSSISLWMEEFQTQETESPQQSKWPPLSADIIEQGTCCHSHMRNEILDKLARSGIFLASELEDFERLLLRLSRIGGVMQDSLPVQSYKSRAGCDFHSRFILYCLEHGLQHLLYTYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQNEDSENCLKKVDPQLLKVALTPYPKLKAALFPQYTAPSILPSDITLYHLIQSLPPFDPSRLFVWQSANTLAIGDTGSQLPHFSSPDLVSKYAVLERLNYAYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFTNPSVGAACVCFLELLGLSSLKLRVDLKMANVILGSKRRDEDARSSFIRESLAEKLSKLADGDRAATEELLVLLEEGVWDSIEQQGFSRLSSESSSQWALVLQFCMLHDRKLSVSYLRECAKANDWLQFLVHSQLHNYHPAEVESLLQYFSPVLQSHLKLASEKLSSGSISRDDSCLQELQKNKGETSNFFEILHRCSDESTSWSWLLAEAVRHRAPILSVLASCVQGASVVSCLCVWIVTSVEDKVAAEAMGHIQISVEDHHWSLKDLSIIWRTVLTRRKSHTLIRGFQLFIKDSPLLLIMEMYELCMFFKNYEKAKVKLLEFQKSLETLDTVAARVLPIIPASWMKDQVCFLLKLMPQQCETQYELGKLLQLFVGTEQLFSDGPDVQKLCLLSQVLKDTPIAISPAVISSYSTENFQRECRSILEKLKADGQFAVARRVAELAALPVDSLLIEQLTQEMQTLTHTPQWSLKQERLGFWKKCHEIFKKNSISKRAASSFFSSQAPEVSEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEGLEKQIWICRVAQHTCGGAEEEAKPSLSQHKLAAAELSFDSLASELSFSKLAALNTSKYLGLNDLPSKTTCENRLKHKEQESLNTLIGQLLDGGCVHEASRVCQYFRFYSQDLVLVLHCRALASAEATMEDLHSEIRALLSSAAQPEDLESPSVPLRKAHSSSSLDSQSFVMVPPTDEVAQNLHTLISKCLHGKNYCRQVLCLYELAKDLGCSYGDVAARDSEAMLRAILASQRPDRCRQAQVFINTQGLEADTVAELVAEEVTRELLTPSEGTGEKQPFNPAEESQTFLQLTALCQDRTLF

Target ORF information:

RefSeq Version	XM_006499109.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 11 (Spg11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499109.3

ORF Insert Sequence:

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg11 ( XM_011239422.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237724.1 Mus musculus spastic paraplegia 11 (Spg11), transcript variant X1, mRNA.

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CloneID	OMu72892
Clone ID Related Accession (Same CDS sequence)	XM_011239422.3 , XM_011239422.2
Accession Version	XM_011239422.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGGTAG GCATGAAGTT GTTGGACAAG ATTCCCTCTG TCCCCCACGG GGAGCTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAC CACTGCTTTA CCTTCACGTG CCACATGGAA 
GGCATCACCA GAGTCCTGCA AGCCGCCCGC ATGCTCACAG ATAACCACTT GGCTCCCAAT 
GAGGAGTACG GATTAGTGGT CCGCCTCCTC ACTGGCATCG GGCGCTACAA TGAGATGACG 
TACATATTTG ACTTGCTGCA TCAAAAGCAT TATTTCGAAG TTCTGATGAG AAAGAAGCTA 
GACCCAGTAG GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237724.1
CDS	406..6963
Translation	MAAQPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCCLPGPFRQFLWEESQNSTSRDRPKLLVAREDYELLVYEFDLRDGRCDAALLHGCCGRTLQKLTEDQGVSISFKSLRILSFHNNTSLLLINRCLILRVVFPGKEPGVPVLDCLSLPLPAQAADMIIDAQLCERFLFVLSTVGLIYIFNTMDGTQVAQVDLALLREDSEQLEPASVSSFTSLRVSQDLDVLVLVSSASTAVALNLHLYFRQHPGHLLCEGTLEDLPIEGPPGIDEDDLVNSAHNMKLSKVSFQVDRSWRAQLLSLNESVRGSEPEVSCCAPWFQSALRLESLESADHTPTVPTHVFIPGDVPRGRCAFPQKEHVKSSDPGRPWKTMHLSEHEQPTELTCLSVTGFTALFTWAVGATSCTIGLWDLETQSMQCFSLSQKCTPVDIGGDQQLCLALTDDGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSQSPAPDQQHPFSSHLYLRQVEEMSPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQIRELCVHTEELDEHFQKGVAILTSYINELRTFMIKFPWKPGDAIDESDVNEGVVTVKEDRVWEELSFEEVIADAILNNRIPEAQTFFRISGHSAQRLEELVRIGLDLAFDSLKKNNVEEASRLLRNMGFSVEDELLKICFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQLEALYSGHFQENAQTQAFPRYWIKEQDCFKHKSVLDTFLKYDKKDEFHKQDHRIALNWAHRWDLQTQECILLRRLSPEEYKARSPEALWRHLTARHDCSSISLWMEEFQTQETESPQQSKWPPLSADIIEQGTCCHSHMRNEILDKLARSGIFLASELEDFERLLLRLSRIGGVMQDSLPVQSYKSRAGCDFHSRFILYCLEHGLQHLLYTYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQNEDSENCLKKVDPQLLKVALTPYPKLKAALFPQYTAPSILPSDITLYHLIQSLPPFDPSRLFVWQSANTLAIGDTGSQLPHFSSPDLVSKYAVLERLNYAYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFTNPSVGAACVCFLELLGLSSLKLRVDLKMANVILGSKRRDEDARSSFIRESLAEKLSKLADGDRAATEELLVLLEEGVWDSIEQQGFSRLSSESSSQWALVLQFCMLHDRKLSVSYLRECAKANDWLQFLVHSQLHNYHPAEVESLLQYFSPVLQSHLKLASEKLSSGSISRDDSCLQELQKNKGETSNFFEILHRCSDESTSWSWLLAEAVRHRAPILSVLASCVQGASVVSCLCVWIVTSVEDKVAAEAMGHIQISVEDHHWSLKDLSIIWRTVLTRRKSHTLIRGFQLFIKDSPLLLIMEMYELCMFFKNYEKAKVKLLEFQKSLETLDTVAARVLPIIPASWMKDQVCFLLKLMPQQCETQYELGKLLQLFVGTEQLFSDGPDVQKLCLLSQVLKDTPIAISPAVISSYSTENFQRECRSILEKLKADGQFAVARRVAELAALPVDSLLIEQLTQEMQTLTHTPQWSLKQERLGFWKKCHEIFKKNSISKRAASSFFSSQAPEVSEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEGLEKQIWICRVAQHTCGGAEEEAKPSLSQHKLAAAELSFDSLASELSFSKLAALNTSKYLGLNDLPSKTTCENRLKHKEQESLNTLIGQLLDGGCVHEASRVCQYFRFYSQDLVLVLHCRALASAEATMEDLHSEIRALLSSAAQPEDLESPSVPLRKAHSSSSLDSQSFVMVPPTDEVAQNLHTLISKCLHGKNYCRQVLCLYELAKDLGCSYGDVAARDSEAMLRAILASQRPDRCRQAQVFINTQGLEADTVAELVAEEVTRELLTPSEGTGEKQPFNPAEESQTFLQLTALCQDRTLVGMKLLDKIPSVPHGELSCTTELLILAHHCFTFTCHMEGITRVLQAARMLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHQKHYFEVLMRKKLDPVGV

Target ORF information:

RefSeq Version	XM_011239422.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 11 (Spg11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239422.2

ORF Insert Sequence:

ATGGCTGCCC AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG GGCCATGGAG 
CGGGTCCTGC CCATGCTGCT GGTGCGGATC CCAGCTGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGCAGG GGTGGGAGCA GCTGCTGCCT CCCAGGACCC 
TTTAGGCAGT TCCTATGGGA GGAGTCTCAG AACAGCACAT CAAGAGACAG GCCCAAGCTG 
CTTGTTGCCA GAGAAGATTA TGAGCTGCTC GTATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTATTACA CGGCTGTTGT GGGAGGACAC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GTATTTCCTT CAAGTCTTTG AGAATTCTGT CATTTCACAA CAACACGTCA 
TTACTGCTCA TCAACAGATG TCTCATCCTA CGCGTTGTGT TTCCGGGGAA AGAACCTGGG 
GTCCCAGTGC TGGACTGCCT CTCACTGCCC CTGCCTGCAC AGGCAGCGGA CATGATCATT 
GATGCACAGC TCTGTGAGAG ATTTCTTTTT GTTCTGAGCA CAGTGGGCTT GATCTATATT 
TTTAACACTA TGGATGGCAC GCAGGTAGCG CAAGTGGACC TGGCTCTTCT CAGAGAAGAT 
TCTGAGCAGC TGGAGCCCGC CAGTGTCTCT TCCTTTACAT CGCTGAGAGT GTCTCAGGAC 
TTGGATGTTC TGGTGCTGGT CAGCTCAGCC AGTACTGCAG TTGCTCTTAA CCTACATTTG 
TATTTCAGGC AACATCCGGG ACACCTACTG TGTGAAGGAA CACTGGAAGA CCTTCCTATT 
GAAGGGCCTC CAGGGATAGA TGAAGACGAC CTGGTTAACT CTGCCCACAA CATGAAACTG 
AGCAAGGTGT CCTTTCAAGT TGATAGGTCC TGGAGAGCCC AGCTGTTGTC ACTGAATGAA 
TCAGTGAGGG GCTCGGAACC AGAGGTTTCC TGCTGTGCTC CGTGGTTCCA GAGTGCTTTG 
CGTTTGGAGT CCCTGGAATC TGCTGACCAC ACCCCCACTG TGCCAACCCA CGTTTTCATT 
CCCGGGGATG TCCCACGTGG CCGGTGTGCT TTTCCTCAGA AGGAGCACGT GAAGAGCAGT 
GATCCAGGAA GGCCGTGGAA GACAATGCAC CTGAGTGAAC ATGAGCAGCC CACGGAGCTC 
ACGTGCCTGT CTGTGACGGG ATTTACTGCA CTGTTTACCT GGGCAGTAGG AGCGACCAGC 
TGCACCATCG GCCTCTGGGA CTTGGAGACC CAGAGCATGC AGTGTTTTTC TCTCAGCCAG 
AAGTGTACTC CTGTAGACAT TGGCGGAGAC CAGCAGCTGT GCCTTGCTTT GACAGATGAT 
GGTCTGTCTC TGATTTTGTT TGGTTTAACC CAAGAGGAGT TTTTGAACAG ACTAATGATC 
CACGGCAGCG CCAGCACTGT GGACTCCCTT TGTCATCTCA ACGGTTGGGG ACGGTGCTCA 
ATCCCTATCC ACGCTCTAGA GGCTGGCATA GAAAATCGTC AGCTGGACAC AGTAGATTTC 
TTCTTGAAGA GCAAGGAAAA TCTTCTTACT CCGTCTTCAC AATCTCCTGC TCCTGATCAG 
CAGCATCCCT TTTCATCCCA TCTGTACCTA AGACAGGTAG AAGAGATGAG CCCAGCCCTG 
GATTTACTAT GCTCAGCAAT TAGAGAAAGT GACTCGGAAA CCCAAAGCAA ACACTTTGCT 
GAGCAGCTGC TTCACCTCAC ACTGTCCTTC CTTAACAAAC AGATAAGGGA GCTTTGTGTT 
CACACTGAAG AGCTAGATGA GCATTTTCAG AAAGGAGTGG CCATTTTGAC CAGCTACATC 
AATGAGCTTC GGACTTTTAT GATAAAGTTT CCTTGGAAAC CAGGAGATGC CATAGATGAA 
TCTGATGTAA ATGAAGGTGT CGTCACAGTG AAGGAGGACC GGGTTTGGGA GGAACTCAGC 
TTTGAGGAAG TTATTGCTGA TGCCATTTTA AATAACAGAA TACCAGAGGC ACAAACTTTC 
TTTAGGATTA GTGGTCATTC CGCTCAACGA CTGGAGGAAC TGGTTAGGAT CGGCCTAGAT 
CTGGCATTTG ACAGTTTAAA AAAGAACAAC GTGGAGGAGG CCTCGAGGCT CCTGAGGAAC 
ATGGGCTTCA GTGTGGAAGA TGAGTTGCTT AAGATATGCT TTTATACAAC TGATAAAAAT 
ATACGGGACT TTTTGGTTGA AATTTTAAAA GAAAAGGAAT GTTTTTCTGA AAAAGAAAGA 
AGAACTGTAG ACTTTGTTCA TCAACTTGAA GCACTTTATT CTGGACACTT CCAGGAAAAT 
GCACAGACGC AGGCTTTCCC CAGGTATTGG ATAAAGGAAC AGGACTGTTT CAAACACAAA 
TCTGTCTTGG ACACATTCCT CAAATATGAT AAAAAAGATG AGTTTCACAA ACAGGACCAT 
AGAATTGCAT TGAATTGGGC TCACCGGTGG GATCTCCAAA CACAGGAATG TATCCTTCTC 
CGCAGACTAA GTCCAGAAGA ATACAAAGCG CGCTCCCCGG AAGCCCTGTG GAGACACCTC 
ACGGCTCGCC ATGACTGCTC AAGCATCAGC TTGTGGATGG AAGAATTTCA GACCCAGGAA 
ACCGAGTCAC CTCAGCAGAG CAAGTGGCCC CCTCTGAGTG CTGACATCAT TGAGCAGGGC 
ACTTGCTGCC ACAGTCACAT GAGGAATGAG ATTTTAGACA AGCTGGCCAG AAGTGGCATT 
TTTCTTGCAT CTGAGCTGGA AGACTTTGAA CGTCTCCTCC TGAGGCTGAG CCGGATTGGG 
GGCGTAATGC AGGACAGCCT CCCCGTGCAG AGCTACAAAT CCCGTGCAGG GTGTGACTTT 
CATTCCCGGT TCATCCTCTA CTGTTTAGAG CACGGCCTAC AGCATCTTCT CTACACCTAT 
CTTGACTATT ACAAACTTAG TCCTGGAAAT TGTCCTTTTT TGGAAAAGAA AGAACTACAT 
GAAGCTCACC CTTGGCTTGA ATTTTTGGTT CAATGTCGGC AAGTCTCCAG TAACTTGACA 
GATCCCAAAC TCATCTTTCA GGCTAGCCTT GCAAATGCTC AGATTTTGAT TCCCACCAAT 
CAGGCCAGTG TGAGCAGTAT GCTGTTGGAA GGACACACCC TCCTGGCCCT TGCTACCACC 
ATGTATGCTC CAGGGGGTGT CAGCCAGGTC ATTCAGAATG AAGACAGCGA GAACTGTTTG 
AAGAAAGTGG ACCCCCAGCT ACTGAAGGTG GCTTTAACAC CTTACCCAAA GCTCAAAGCT 
GCGCTCTTCC CACAGTACAC TGCTCCTAGT ATTCTGCCCT CTGATATTAC ACTCTACCAC 
CTCATCCAGT CCTTGCCGCC CTTTGATCCT AGCCGGCTGT TCGTCTGGCA GTCTGCCAAC 
ACACTAGCTA TAGGAGACAC AGGCAGTCAG CTCCCACACT TTTCCAGCCC TGACCTGGTG 
AGTAAATACG CTGTGCTGGA ACGGCTGAAC TACGCGTATT ATCTACATCA CGGACGCCCG 
TCGTTTGCCT TTGGCACCTT TCTGGTCCAG GAGTTGATCA AGAGTAAGAC TCCCAAACAA 
CTGATCCAGC AAGTAGGCAA GGAGGCCTAT ACCTTAGGGC TCTCTTCCTT CACCAACCCT 
TCGGTAGGGG CTGCCTGTGT CTGCTTCTTA GAATTGCTTG GCCTCAGCAG CCTCAAGCTC 
AGAGTTGACC TGAAAATGGC AAATGTAATT TTGGGCTCCA AGCGTAGAGA TGAAGATGCT 
CGTTCCAGCT TCATCAGAGA GTCTCTGGCT GAGAAGCTGT CTAAGCTTGC TGACGGTGAC 
AGAGCAGCCA CAGAAGAGCT GCTTGTTCTC TTGGAGGAAG GCGTGTGGGA CAGCATTGAG 
CAGCAGGGCT TTAGCAGGTT GTCCAGTGAG TCCAGCAGCC AGTGGGCATT AGTGCTTCAG 
TTCTGCATGC TCCATGACAG GAAGCTGAGC GTGTCCTACC TTAGAGAATG TGCCAAAGCC 
AATGACTGGC TGCAGTTCCT GGTCCACAGC CAGCTGCATA ACTACCACCC AGCAGAGGTG 
GAATCTCTTC TTCAGTATTT CAGTCCTGTC CTTCAGAGCC ACTTGAAGCT GGCTTCTGAG 
AAACTGTCAT CAGGGTCCAT CTCTAGGGAT GACAGCTGCC TCCAGGAACT CCAGAAAAAC 
AAAGGAGAAA CGAGTAATTT CTTTGAAATT CTCCACCGGT GCTCAGATGA GTCCACCTCT 
TGGAGCTGGC TCCTGGCTGA GGCAGTGAGA CATCGGGCTC CCATCCTCAG TGTCCTGGCC 
TCGTGTGTGC AGGGTGCCAG TGTTGTCTCC TGTCTCTGTG TTTGGATTGT CACCTCCGTG 
GAGGACAAGG TTGCAGCTGA AGCAATGGGA CACATTCAGA TCTCTGTAGA GGACCATCAC 
TGGAGCCTGA AGGACCTGTC CATCATCTGG AGAACTGTAT TGACAAGACG CAAGAGCCAC 
ACTCTCATCC GAGGTTTCCA GCTCTTTATT AAGGATTCCC CACTTCTATT GATTATGGAG 
ATGTATGAAC TGTGTATGTT CTTCAAGAAT TACGAGAAAG CCAAAGTTAA ACTTTTGGAG 
TTCCAGAAAA GCCTTGAAAC ACTTGACACA GTGGCCGCAA GGGTCCTTCC TATCATACCT 
GCCTCGTGGA TGAAAGACCA GGTTTGTTTC CTTTTGAAAC TTATGCCGCA GCAGTGTGAG 
ACGCAGTATG AGCTGGGGAA ACTCCTCCAA CTGTTTGTTG GCACTGAGCA GCTCTTCTCT 
GACGGGCCAG ACGTGCAGAA GCTGTGTCTC CTCAGCCAAG TGCTGAAGGA CACACCTATC 
GCCATTAGCC CTGCAGTCAT TTCCAGCTAC AGCACTGAGA ACTTTCAGCG GGAATGTAGA 
TCTATTTTGG AGAAGCTAAA AGCAGATGGG CAGTTTGCTG TGGCTAGGAG AGTGGCCGAG 
TTAGCAGCCC TGCCTGTGGA CAGCTTGCTG ATTGAACAGT TAACTCAGGA AATGCAAACA 
CTAACACACA CTCCGCAGTG GTCCCTGAAA CAAGAAAGAC TTGGCTTCTG GAAAAAATGC 
CATGAAATTT TTAAGAAAAA CTCTATTTCA AAGAGAGCAG CCTCTTCCTT CTTCTCTAGC 
CAAGCCCCCG AGGTGAGTGA GCACCCTGCT GAACAGGGCA GCCTTGAGGA GCGCCATCTG 
CTGCTCACTC TGGCTGGCCA CTGGCTTGCC CAAGAAGAGC CGGTTCCCGT GGAGGAACTG 
GAGGGGCTGG AGAAACAGAT TTGGATCTGC CGCGTCGCCC AGCACACATG TGGAGGGGCT 
GAAGAGGAAG CCAAGCCCAG CCTCTCTCAA CACAAGTTGG CTGCTGCTGA ACTTTCTTTT 
GATAGCTTAG CCAGTGAGCT GTCCTTCTCC AAGCTCGCTG CTTTGAACAC ATCCAAATAC 
TTAGGCCTTA ATGATCTTCC ATCCAAGACG ACGTGTGAGA ACAGACTGAA ACACAAAGAG 
CAGGAGTCAC TGAACACTTT GATCGGGCAG CTGCTGGATG GTGGCTGTGT GCATGAAGCA 
AGCAGAGTGT GCCAGTATTT CCGTTTCTAT AGCCAGGATC TAGTGCTGGT GTTGCACTGC 
AGAGCCTTGG CCTCCGCAGA AGCCACCATG GAAGACTTGC ACTCAGAGAT CCGTGCCCTC 
CTCTCAAGTG CCGCACAGCC TGAGGACCTG GAGAGCCCGA GTGTCCCTCT TCGGAAAGCC 
CATAGCTCTT CAAGTTTGGA CAGTCAGTCA TTTGTGATGG TACCACCCAC TGATGAGGTA 
GCCCAGAACC TCCACACTCT CATAAGCAAG TGCCTCCATG GGAAGAACTA CTGCCGGCAG 
GTCCTCTGTC TGTATGAACT TGCCAAGGAT TTGGGCTGTT CCTATGGAGA TGTTGCTGCT 
AGGGACAGTG AAGCCATGCT CCGAGCAATC TTGGCTTCTC AGCGGCCTGA CCGATGTAGA 
CAAGCCCAGG TCTTCATCAA CACCCAGGGC CTTGAAGCAG ACACTGTGGC TGAACTAGTG 
GCTGAAGAGG TGACACGGGA GCTACTGACT CCATCAGAGG GAACAGGAGA AAAGCAACCT 
TTCAACCCAG CAGAGGAAAG CCAGACATTT CTTCAGCTAA CTGCTTTGTG TCAAGACCGT 
ACATTGGTAG GCATGAAGTT GTTGGACAAG ATTCCCTCTG TCCCCCACGG GGAGCTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAC CACTGCTTTA CCTTCACGTG CCACATGGAA 
GGCATCACCA GAGTCCTGCA AGCCGCCCGC ATGCTCACAG ATAACCACTT GGCTCCCAAT 
GAGGAGTACG GATTAGTGGT CCGCCTCCTC ACTGGCATCG GGCGCTACAA TGAGATGACG 
TACATATTTG ACTTGCTGCA TCAAAAGCAT TATTTCGAAG TTCTGATGAG AAAGAAGCTA 
GACCCAGTAG GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of spatacsin function alters lysosomal lipid clearance leading to upper and lower motor neuron degeneration.
Neurobiology of disease10221-37(2017 Jun)
Branchu J,Boutry M,Sourd L,Depp M,Leone C,Corriger A,Vallucci M,Esteves T,Matusiak R,Dumont M,Muriel MP,Santorelli FM,Brice A,El Hachimi KH,Stevanin G,Darios F

In Vivo Evidence for Lysosome Depletion and Impaired Autophagic Clearance in Hereditary Spastic Paraplegia Type SPG11.
PLoS genetics11(8)e1005454(2015 Aug)
Varga RE,Khundadze M,Damme M,Nietzsche S,Hoffmann B,Stauber T,Koch N,Hennings JC,Franzka P,Huebner AK,Kessels MM,Biskup C,Jentsch TJ,Qualmann B,Braulke T,Kurth I,Beetz C,H?bner CA

Dysfunction of spatacsin leads to axonal pathology in SPG11-linked hereditary spastic paraplegia.
Human molecular genetics23(18)4859-74(2014 Sep)
P?rez-Brangul? F,Mishra HK,Prots I,Havlicek S,Kohl Z,Saul D,Rummel C,Dorca-Arevalo J,Regensburger M,Graef D,Sock E,Blasi J,Groemer TW,Schl?tzer-Schrehardt U,Winkler J,Winner B

Cellular distribution and subcellular localization of spatacsin and spastizin, two proteins involved in hereditary spastic paraplegia.
Molecular and cellular neurosciences47(3)191-202(2011 Jul)
Murmu RP,Martin E,Rastetter A,Esteves T,Muriel MP,El Hachimi KH,Denora PS,Dauphin A,Fernandez JC,Duyckaerts C,Brice A,Darios F,Stevanin G

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

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Spg11 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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