gRNAs
The following Tnrc6b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnrc6b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu11110 | |
| Clone ID Related Accession (Same CDS sequence) | NM_144812.2 , NM_144812.3 | |
| Accession Version | NM_144812.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-26 | |
| Organism | Mus musculus(house mouse) | |
| Product | trinucleotide repeat-containing gene 6B protein isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK048628.1, BC068158.1, AK052247.1, BC017531.1 and AC125540.4. On Jun 15, 2005 this sequence version replaced NM_144812.1. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCAAACCA ATGAGGGAGA AGTAGAGGAA GAAAGCAGCT CCCAGGTGGA ACAGGAGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_659061.2 |
| CDS | 292..5724 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_144812.2 |
1 | ATGCAAACCA ATGAGGGAGA AGTAGAGGAA GAAAGCAGCT CCCAGGTGGA ACAGGAGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu11110 | |
| Clone ID Related Accession (Same CDS sequence) | NM_144812.2 , NM_144812.3 | |
| Accession Version | NM_144812.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | trinucleotide repeat-containing gene 6B protein isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126682.3 and AC125540.4. On Aug 17, 2018 this sequence version replaced NM_144812.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR1660811.163195.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCAAACCA ATGAGGGAGA AGTAGAGGAA GAAAGCAGCT CCCAGGTGGA ACAGGAGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_659061.2 |
| CDS | 291..5723 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_144812.3 |
1 | ATGCAAACCA ATGAGGGAGA AGTAGAGGAA GAAAGCAGCT CCCAGGTGGA ACAGGAGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu58422 | |
| Clone ID Related Accession (Same CDS sequence) | NM_177124.4 | |
| Accession Version | NM_177124.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-09 | |
| Organism | Mus musculus(house mouse) | |
| Product | trinucleotide repeat-containing gene 6B protein isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK639087.1, CN699186.1, AC126682.3, CN704499.1, AK162702.1 and AC125540.4. On Nov 2, 2007 this sequence version replaced NM_177124.3. Transcript Variant: This variant (2) has multiple differences in the 5' UTR and 5' coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849390, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_796098.3 |
| CDS | 191..5515 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_177124.4 |
1 | ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung. |
Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice. |
Trinucleotide repeat containing 6a (Tnrc6a)-mediated microRNA function is required for development of yolk sac endoderm. |
Ago-TNRC6 triggers microRNA-mediated decay by promoting two deadenylation steps. |
