Chrm5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrm5
Entrez Gene ID	213788
Full Name	cholinergic receptor, muscarinic 5
Synonyms	AChR-M5,M5R,m5
General protein information	Preferred Names cholinergic receptor, muscarinic 5 Names muscarinic acetylcholine receptor M5 muscarinic acetylcholine receptor 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E3\|2 57.02 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499099.3	XP_006499162.1	muscarinic acetylcholine receptor M5 isoform X1
NM_205783.2	NP_991352.2	muscarinic acetylcholine receptor M5
XM_006499099.4	XP_006499162.1	muscarinic acetylcholine receptor M5 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHRM5	XP_003952673.1
Canis lupus familiaris (dog)	CHRM5	XP_005638247.1
Bos taurus (cattle)	CHRM5	NP_001231540.1
	CHRM5	NP_001028103.1
Rattus norvegicus (Norway rat)	Chrm5	NP_059058.1
Gallus gallus (chicken)	CHRM5	NP_001026721.1
Homo sapiens (human)	CHRM5	NP_036257.1
Mus musculus (house mouse)	Chrm5	NP_991352.2
Danio rerio (zebrafish)	chrm5a	NP_001018639.1
Xenopus tropicalis (tropical clawed frog)	chrm5	XP_002935387.2

Gene Ontology
Bibliography

Related articles in PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Differential regulation of primary afferent input to spinal cord by muscarinic receptor subtypes delineated using knockout mice.
Chen SR, Chen H, Yuan WX, Wess J, Pan HL
The Journal of biological chemistry289(20)14321-30(2014 May)

M5 receptor activation produces opposing physiological outcomes in dopamine neurons depending on the receptor's location.
Foster DJ, Gentry PR, Lizardi-Ortiz JE, Bridges TM, Wood MR, Niswender CM, Sulzer D, Lindsley CW, Xiang Z, Conn PJ
The Journal of neuroscience : the official journal of the Society for Neuroscience34(9)3253-62(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonistrated that activation of M5 receptors in the striatum induces an inhibition in dopamine release.
Title: M5 receptor activation produces opposing physiological outcomes in dopamine neurons depending on the receptor's location.

In the absence of VTA M(5) receptors, ventral tegmental area cholinergic agent inputs inhibit locomotion.
Title: M5 muscarinic receptor knockout mice show reduced morphine-induced locomotion but increased locomotion after cholinergic antagonism in the ventral tegmental area.

M2 and M5 muscarinic receptors but not M4 muscarinic receptor or D2 dopamine receptors are needed for male USV production during male-female interactions
Title: Ultrasonic vocalizations induced by sex and amphetamine in M2, M4, M5 muscarinic and D2 dopamine receptor knockout mice.

Results suggest that M1 and M5 muscarinic cholinergic receptors are not involved in anti-viral immunity.
Title: Analysis of CD8+ T cell-mediated anti-viral responses in mice with targeted deletions of the M1 or M5 muscarinic cholinergic receptors.

Male M5R-/- mice exhibited a significantly reduced cerebral blood flow (CBF) in the cerebral cortex, hippocampus, basal ganglia, and thalamus. Cortical and hippocampal pyramidal neurons from M5R-/- mice showed neuronal atrophy.
Title: Loss of M5 muscarinic acetylcholine receptors leads to cerebrovascular and neuronal abnormalities and cognitive deficits in mice.

The following Chrm5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrm5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19635	NM_205783.2 Latest version!	Mus musculus cholinergic receptor, muscarinic 5 (Chrm5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19635	XM_006499099.4 Latest version!	Mus musculus cholinergic receptor, muscarinic 5 (Chrm5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu19635	XM_006499099.3	Mus musculus cholinergic receptor, muscarinic 5 (Chrm5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrm5 ( NM_205783.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_991352.2 Mus musculus cholinergic receptor, muscarinic 5 (Chrm5), mRNA.

Price & Availability↑

CloneID	OMu19635
Clone ID Related Accession (Same CDS sequence)	XM_006499099.3 , NM_205783.2 , XM_006499099.4
Accession Version	NM_205783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	muscarinic acetylcholine receptor M5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731840.17. On Jul 24, 2009 this sequence version replaced NM_205783.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC120615.1 [ECO:0000345] ##Evidence-Data-END##

ATGGAAGGGG AGTCTTATCA CAATGAAACC ACTGTCAACG GCACCCCAGT AAATCACCAG 
GCTTTGGAAC GCCATGGACT GTGGGAAGTC ATTACTATTG CAGCTGTGAC CGCTGTGGTC 
AGTCTGATGA CCATTGTCGG CAATGTCTTG GTCATGATCT CCTTCAAAGT CAACAGTCAG 
CTCAAGACAG TTAACAACTA TTACCTGCTC AGCTTGGCCT GTGCAGACCT CATCATTGGC 
ATCTTCTCCA TGAACCTCTA CACGACCTAC ATCCTCATGG GACGCTGGGT TCTCGGGAGT 
CTGGCCTGTG ACCTTTGGCT TGCACTCGAC TATGTAGCCA GCAACGCTTC TGTCATGAAC 
CTTCTGGTGA TTAGCTTTGA TCGTTACTTT TCCATCACAA GACCACTGAC ATACCGAGCC 
AAGCGTACCC CAAAGAGGGC TGGCATCATG ATCGGCTTGG CATGGCTGGT CTCCTTCATC 
CTCTGGGCGC CAGCCATCCT CTGCTGGCAG TACTTGGTCG GGAAGCGGAC AGTACCACCT 
GATGAGTGCC AGATCCAGTT CCTCTCTGAG CCCACCATCA CTTTTGGGAC CGCCATTGCC 
GCTTTCTATA TCCCTGTCTC CGTCATGACC ATACTCTACT GCCGGATCTA CCGGGAAACA 
GAGAAACGAA CCAAGGACCT GGCTGACCTC CAAGGTTCCG ATTCTGTGGC AGAAGTCAAG 
AAGAGAAAAC CGGCTCACAG GACCCTGCTC AGATCTTTCT TTAGCTGCCC TAGACCCAGC 
CTGGCCCAGA GAGTACGGAA CCAGGCCTCC TGGTCATCCT CCCGTAGAAG CACCTCAACA 
ACGGGAAAGC CAACCCAGGC CACTGATCTA AGTGCTGACT GGGAAAAGGC TGAGCAGGTT 
ACCAACTGTA GCAGCTGCCC CTCTTCAGAG GACGAAGCCA AGGCCACCAC TGACCCTGTC 
TTTCAAGTGG TCTGCAAGAA TGAGGCCAAG GAAAGCCCGG GGAAGGAATT CAATACCCAA 
GAGACCAAGG AAACGTTTGT GAGCCCTCGG ACTGAAAACA ATGACTATGA CACTCCCAAG 
TACTTCCTGT CTCCAGGTGC TGCTCACAGA CTCAAGAGTC AGAAGTGTGT TGCCTATAAG 
TTCCGATTGG TGGTAAAAGC TGACGGGACC CAGGAGACAA ACAATGGCTG TCGTAAGGTG 
AAAATCATGC CCTGTTCCTT CCCAGTGTCC AAAGACCCTT CAACAAAAGG CCTGGATCCC 
CACCTCAGCC ATCAAATGAC CAAACGAAAG AGAATGGTCC TAGTCAAAGA GAGGAAAGCG 
GCGCAGACCT TGAGCGCCAT TCTCCTGGCC TTCATCATCA CATGGACTCC TTATAACATC 
ATGGTCCTGG TTTCCACCTT CTGTGACAAG TGTGTCCCTG TCACCCTGTG GCACTTGGGT 
TACTGGCTGT GCTATGTCAA CAGCACCATC AACCCCATCT GTTACGCTCT CTGCAACAGA 
ACCTTCAGGA AGACCTTTAA GCTGCTGCTT CTCTGCCGGT GGAAAAAGAA AAAAGTAGAA 
GAGAAATTGT ATTGGCAAGG CAACAGCAAG CTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991352.2
CDS	49..1647
Translation	MEGESYHNETTVNGTPVNHQALERHGLWEVITIAAVTAVVSLMTIVGNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTTYILMGRWVLGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWLVSFILWAPAILCWQYLVGKRTVPPDECQIQFLSEPTITFGTAIAAFYIPVSVMTILYCRIYRETEKRTKDLADLQGSDSVAEVKKRKPAHRTLLRSFFSCPRPSLAQRVRNQASWSSSRRSTSTTGKPTQATDLSADWEKAEQVTNCSSCPSSEDEAKATTDPVFQVVCKNEAKESPGKEFNTQETKETFVSPRTENNDYDTPKYFLSPGAAHRLKSQKCVAYKFRLVVKADGTQETNNGCRKVKIMPCSFPVSKDPSTKGLDPHLSHQMTKRKRMVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTINPICYALCNRTFRKTFKLLLLCRWKKKKVEEKLYWQGNSKLP

Target ORF information:

RefSeq Version	NM_205783.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, muscarinic 5 (Chrm5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205783.2

ORF Insert Sequence:

ATGGAAGGGG AGTCTTATCA CAATGAAACC ACTGTCAACG GCACCCCAGT AAATCACCAG 
GCTTTGGAAC GCCATGGACT GTGGGAAGTC ATTACTATTG CAGCTGTGAC CGCTGTGGTC 
AGTCTGATGA CCATTGTCGG CAATGTCTTG GTCATGATCT CCTTCAAAGT CAACAGTCAG 
CTCAAGACAG TTAACAACTA TTACCTGCTC AGCTTGGCCT GTGCAGACCT CATCATTGGC 
ATCTTCTCCA TGAACCTCTA CACGACCTAC ATCCTCATGG GACGCTGGGT TCTCGGGAGT 
CTGGCCTGTG ACCTTTGGCT TGCACTCGAC TATGTAGCCA GCAACGCTTC TGTCATGAAC 
CTTCTGGTGA TTAGCTTTGA TCGTTACTTT TCCATCACAA GACCACTGAC ATACCGAGCC 
AAGCGTACCC CAAAGAGGGC TGGCATCATG ATCGGCTTGG CATGGCTGGT CTCCTTCATC 
CTCTGGGCGC CAGCCATCCT CTGCTGGCAG TACTTGGTCG GGAAGCGGAC AGTACCACCT 
GATGAGTGCC AGATCCAGTT CCTCTCTGAG CCCACCATCA CTTTTGGGAC CGCCATTGCC 
GCTTTCTATA TCCCTGTCTC CGTCATGACC ATACTCTACT GCCGGATCTA CCGGGAAACA 
GAGAAACGAA CCAAGGACCT GGCTGACCTC CAAGGTTCCG ATTCTGTGGC AGAAGTCAAG 
AAGAGAAAAC CGGCTCACAG GACCCTGCTC AGATCTTTCT TTAGCTGCCC TAGACCCAGC 
CTGGCCCAGA GAGTACGGAA CCAGGCCTCC TGGTCATCCT CCCGTAGAAG CACCTCAACA 
ACGGGAAAGC CAACCCAGGC CACTGATCTA AGTGCTGACT GGGAAAAGGC TGAGCAGGTT 
ACCAACTGTA GCAGCTGCCC CTCTTCAGAG GACGAAGCCA AGGCCACCAC TGACCCTGTC 
TTTCAAGTGG TCTGCAAGAA TGAGGCCAAG GAAAGCCCGG GGAAGGAATT CAATACCCAA 
GAGACCAAGG AAACGTTTGT GAGCCCTCGG ACTGAAAACA ATGACTATGA CACTCCCAAG 
TACTTCCTGT CTCCAGGTGC TGCTCACAGA CTCAAGAGTC AGAAGTGTGT TGCCTATAAG 
TTCCGATTGG TGGTAAAAGC TGACGGGACC CAGGAGACAA ACAATGGCTG TCGTAAGGTG 
AAAATCATGC CCTGTTCCTT CCCAGTGTCC AAAGACCCTT CAACAAAAGG CCTGGATCCC 
CACCTCAGCC ATCAAATGAC CAAACGAAAG AGAATGGTCC TAGTCAAAGA GAGGAAAGCG 
GCGCAGACCT TGAGCGCCAT TCTCCTGGCC TTCATCATCA CATGGACTCC TTATAACATC 
ATGGTCCTGG TTTCCACCTT CTGTGACAAG TGTGTCCCTG TCACCCTGTG GCACTTGGGT 
TACTGGCTGT GCTATGTCAA CAGCACCATC AACCCCATCT GTTACGCTCT CTGCAACAGA 
ACCTTCAGGA AGACCTTTAA GCTGCTGCTT CTCTGCCGGT GGAAAAAGAA AAAAGTAGAA 
GAGAAATTGT ATTGGCAAGG CAACAGCAAG CTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrm5 ( XM_006499099.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499162.1 Mus musculus cholinergic receptor, muscarinic 5 (Chrm5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19635
Clone ID Related Accession (Same CDS sequence)	XM_006499099.3 , NM_205783.2 , XM_006499099.4
Accession Version	XM_006499099.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	muscarinic acetylcholine receptor M5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGG AGTCTTATCA CAATGAAACC ACTGTCAACG GCACCCCAGT AAATCACCAG 
GCTTTGGAAC GCCATGGACT GTGGGAAGTC ATTACTATTG CAGCTGTGAC CGCTGTGGTC 
AGTCTGATGA CCATTGTCGG CAATGTCTTG GTCATGATCT CCTTCAAAGT CAACAGTCAG 
CTCAAGACAG TTAACAACTA TTACCTGCTC AGCTTGGCCT GTGCAGACCT CATCATTGGC 
ATCTTCTCCA TGAACCTCTA CACGACCTAC ATCCTCATGG GACGCTGGGT TCTCGGGAGT 
CTGGCCTGTG ACCTTTGGCT TGCACTCGAC TATGTAGCCA GCAACGCTTC TGTCATGAAC 
CTTCTGGTGA TTAGCTTTGA TCGTTACTTT TCCATCACAA GACCACTGAC ATACCGAGCC 
AAGCGTACCC CAAAGAGGGC TGGCATCATG ATCGGCTTGG CATGGCTGGT CTCCTTCATC 
CTCTGGGCGC CAGCCATCCT CTGCTGGCAG TACTTGGTCG GGAAGCGGAC AGTACCACCT 
GATGAGTGCC AGATCCAGTT CCTCTCTGAG CCCACCATCA CTTTTGGGAC CGCCATTGCC 
GCTTTCTATA TCCCTGTCTC CGTCATGACC ATACTCTACT GCCGGATCTA CCGGGAAACA 
GAGAAACGAA CCAAGGACCT GGCTGACCTC CAAGGTTCCG ATTCTGTGGC AGAAGTCAAG 
AAGAGAAAAC CGGCTCACAG GACCCTGCTC AGATCTTTCT TTAGCTGCCC TAGACCCAGC 
CTGGCCCAGA GAGTACGGAA CCAGGCCTCC TGGTCATCCT CCCGTAGAAG CACCTCAACA 
ACGGGAAAGC CAACCCAGGC CACTGATCTA AGTGCTGACT GGGAAAAGGC TGAGCAGGTT 
ACCAACTGTA GCAGCTGCCC CTCTTCAGAG GACGAAGCCA AGGCCACCAC TGACCCTGTC 
TTTCAAGTGG TCTGCAAGAA TGAGGCCAAG GAAAGCCCGG GGAAGGAATT CAATACCCAA 
GAGACCAAGG AAACGTTTGT GAGCCCTCGG ACTGAAAACA ATGACTATGA CACTCCCAAG 
TACTTCCTGT CTCCAGGTGC TGCTCACAGA CTCAAGAGTC AGAAGTGTGT TGCCTATAAG 
TTCCGATTGG TGGTAAAAGC TGACGGGACC CAGGAGACAA ACAATGGCTG TCGTAAGGTG 
AAAATCATGC CCTGTTCCTT CCCAGTGTCC AAAGACCCTT CAACAAAAGG CCTGGATCCC 
CACCTCAGCC ATCAAATGAC CAAACGAAAG AGAATGGTCC TAGTCAAAGA GAGGAAAGCG 
GCGCAGACCT TGAGCGCCAT TCTCCTGGCC TTCATCATCA CATGGACTCC TTATAACATC 
ATGGTCCTGG TTTCCACCTT CTGTGACAAG TGTGTCCCTG TCACCCTGTG GCACTTGGGT 
TACTGGCTGT GCTATGTCAA CAGCACCATC AACCCCATCT GTTACGCTCT CTGCAACAGA 
ACCTTCAGGA AGACCTTTAA GCTGCTGCTT CTCTGCCGGT GGAAAAAGAA AAAAGTAGAA 
GAGAAATTGT ATTGGCAAGG CAACAGCAAG CTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499162.1
CDS	3242..4840
Translation	MEGESYHNETTVNGTPVNHQALERHGLWEVITIAAVTAVVSLMTIVGNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTTYILMGRWVLGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWLVSFILWAPAILCWQYLVGKRTVPPDECQIQFLSEPTITFGTAIAAFYIPVSVMTILYCRIYRETEKRTKDLADLQGSDSVAEVKKRKPAHRTLLRSFFSCPRPSLAQRVRNQASWSSSRRSTSTTGKPTQATDLSADWEKAEQVTNCSSCPSSEDEAKATTDPVFQVVCKNEAKESPGKEFNTQETKETFVSPRTENNDYDTPKYFLSPGAAHRLKSQKCVAYKFRLVVKADGTQETNNGCRKVKIMPCSFPVSKDPSTKGLDPHLSHQMTKRKRMVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTINPICYALCNRTFRKTFKLLLLCRWKKKKVEEKLYWQGNSKLP

Target ORF information:

RefSeq Version	XM_006499099.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, muscarinic 5 (Chrm5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499099.3

ORF Insert Sequence:

ATGGAAGGGG AGTCTTATCA CAATGAAACC ACTGTCAACG GCACCCCAGT AAATCACCAG 
GCTTTGGAAC GCCATGGACT GTGGGAAGTC ATTACTATTG CAGCTGTGAC CGCTGTGGTC 
AGTCTGATGA CCATTGTCGG CAATGTCTTG GTCATGATCT CCTTCAAAGT CAACAGTCAG 
CTCAAGACAG TTAACAACTA TTACCTGCTC AGCTTGGCCT GTGCAGACCT CATCATTGGC 
ATCTTCTCCA TGAACCTCTA CACGACCTAC ATCCTCATGG GACGCTGGGT TCTCGGGAGT 
CTGGCCTGTG ACCTTTGGCT TGCACTCGAC TATGTAGCCA GCAACGCTTC TGTCATGAAC 
CTTCTGGTGA TTAGCTTTGA TCGTTACTTT TCCATCACAA GACCACTGAC ATACCGAGCC 
AAGCGTACCC CAAAGAGGGC TGGCATCATG ATCGGCTTGG CATGGCTGGT CTCCTTCATC 
CTCTGGGCGC CAGCCATCCT CTGCTGGCAG TACTTGGTCG GGAAGCGGAC AGTACCACCT 
GATGAGTGCC AGATCCAGTT CCTCTCTGAG CCCACCATCA CTTTTGGGAC CGCCATTGCC 
GCTTTCTATA TCCCTGTCTC CGTCATGACC ATACTCTACT GCCGGATCTA CCGGGAAACA 
GAGAAACGAA CCAAGGACCT GGCTGACCTC CAAGGTTCCG ATTCTGTGGC AGAAGTCAAG 
AAGAGAAAAC CGGCTCACAG GACCCTGCTC AGATCTTTCT TTAGCTGCCC TAGACCCAGC 
CTGGCCCAGA GAGTACGGAA CCAGGCCTCC TGGTCATCCT CCCGTAGAAG CACCTCAACA 
ACGGGAAAGC CAACCCAGGC CACTGATCTA AGTGCTGACT GGGAAAAGGC TGAGCAGGTT 
ACCAACTGTA GCAGCTGCCC CTCTTCAGAG GACGAAGCCA AGGCCACCAC TGACCCTGTC 
TTTCAAGTGG TCTGCAAGAA TGAGGCCAAG GAAAGCCCGG GGAAGGAATT CAATACCCAA 
GAGACCAAGG AAACGTTTGT GAGCCCTCGG ACTGAAAACA ATGACTATGA CACTCCCAAG 
TACTTCCTGT CTCCAGGTGC TGCTCACAGA CTCAAGAGTC AGAAGTGTGT TGCCTATAAG 
TTCCGATTGG TGGTAAAAGC TGACGGGACC CAGGAGACAA ACAATGGCTG TCGTAAGGTG 
AAAATCATGC CCTGTTCCTT CCCAGTGTCC AAAGACCCTT CAACAAAAGG CCTGGATCCC 
CACCTCAGCC ATCAAATGAC CAAACGAAAG AGAATGGTCC TAGTCAAAGA GAGGAAAGCG 
GCGCAGACCT TGAGCGCCAT TCTCCTGGCC TTCATCATCA CATGGACTCC TTATAACATC 
ATGGTCCTGG TTTCCACCTT CTGTGACAAG TGTGTCCCTG TCACCCTGTG GCACTTGGGT 
TACTGGCTGT GCTATGTCAA CAGCACCATC AACCCCATCT GTTACGCTCT CTGCAACAGA 
ACCTTCAGGA AGACCTTTAA GCTGCTGCTT CTCTGCCGGT GGAAAAAGAA AAAAGTAGAA 
GAGAAATTGT ATTGGCAAGG CAACAGCAAG CTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrm5 ( XM_006499099.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499162.1 Mus musculus cholinergic receptor, muscarinic 5 (Chrm5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19635
Clone ID Related Accession (Same CDS sequence)	XM_006499099.3 , NM_205783.2 , XM_006499099.4
Accession Version	XM_006499099.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	muscarinic acetylcholine receptor M5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499099.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGG AGTCTTATCA CAATGAAACC ACTGTCAACG GCACCCCAGT AAATCACCAG 
GCTTTGGAAC GCCATGGACT GTGGGAAGTC ATTACTATTG CAGCTGTGAC CGCTGTGGTC 
AGTCTGATGA CCATTGTCGG CAATGTCTTG GTCATGATCT CCTTCAAAGT CAACAGTCAG 
CTCAAGACAG TTAACAACTA TTACCTGCTC AGCTTGGCCT GTGCAGACCT CATCATTGGC 
ATCTTCTCCA TGAACCTCTA CACGACCTAC ATCCTCATGG GACGCTGGGT TCTCGGGAGT 
CTGGCCTGTG ACCTTTGGCT TGCACTCGAC TATGTAGCCA GCAACGCTTC TGTCATGAAC 
CTTCTGGTGA TTAGCTTTGA TCGTTACTTT TCCATCACAA GACCACTGAC ATACCGAGCC 
AAGCGTACCC CAAAGAGGGC TGGCATCATG ATCGGCTTGG CATGGCTGGT CTCCTTCATC 
CTCTGGGCGC CAGCCATCCT CTGCTGGCAG TACTTGGTCG GGAAGCGGAC AGTACCACCT 
GATGAGTGCC AGATCCAGTT CCTCTCTGAG CCCACCATCA CTTTTGGGAC CGCCATTGCC 
GCTTTCTATA TCCCTGTCTC CGTCATGACC ATACTCTACT GCCGGATCTA CCGGGAAACA 
GAGAAACGAA CCAAGGACCT GGCTGACCTC CAAGGTTCCG ATTCTGTGGC AGAAGTCAAG 
AAGAGAAAAC CGGCTCACAG GACCCTGCTC AGATCTTTCT TTAGCTGCCC TAGACCCAGC 
CTGGCCCAGA GAGTACGGAA CCAGGCCTCC TGGTCATCCT CCCGTAGAAG CACCTCAACA 
ACGGGAAAGC CAACCCAGGC CACTGATCTA AGTGCTGACT GGGAAAAGGC TGAGCAGGTT 
ACCAACTGTA GCAGCTGCCC CTCTTCAGAG GACGAAGCCA AGGCCACCAC TGACCCTGTC 
TTTCAAGTGG TCTGCAAGAA TGAGGCCAAG GAAAGCCCGG GGAAGGAATT CAATACCCAA 
GAGACCAAGG AAACGTTTGT GAGCCCTCGG ACTGAAAACA ATGACTATGA CACTCCCAAG 
TACTTCCTGT CTCCAGGTGC TGCTCACAGA CTCAAGAGTC AGAAGTGTGT TGCCTATAAG 
TTCCGATTGG TGGTAAAAGC TGACGGGACC CAGGAGACAA ACAATGGCTG TCGTAAGGTG 
AAAATCATGC CCTGTTCCTT CCCAGTGTCC AAAGACCCTT CAACAAAAGG CCTGGATCCC 
CACCTCAGCC ATCAAATGAC CAAACGAAAG AGAATGGTCC TAGTCAAAGA GAGGAAAGCG 
GCGCAGACCT TGAGCGCCAT TCTCCTGGCC TTCATCATCA CATGGACTCC TTATAACATC 
ATGGTCCTGG TTTCCACCTT CTGTGACAAG TGTGTCCCTG TCACCCTGTG GCACTTGGGT 
TACTGGCTGT GCTATGTCAA CAGCACCATC AACCCCATCT GTTACGCTCT CTGCAACAGA 
ACCTTCAGGA AGACCTTTAA GCTGCTGCTT CTCTGCCGGT GGAAAAAGAA AAAAGTAGAA 
GAGAAATTGT ATTGGCAAGG CAACAGCAAG CTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499162.1
CDS	2133..3731
Translation	MEGESYHNETTVNGTPVNHQALERHGLWEVITIAAVTAVVSLMTIVGNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTTYILMGRWVLGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWLVSFILWAPAILCWQYLVGKRTVPPDECQIQFLSEPTITFGTAIAAFYIPVSVMTILYCRIYRETEKRTKDLADLQGSDSVAEVKKRKPAHRTLLRSFFSCPRPSLAQRVRNQASWSSSRRSTSTTGKPTQATDLSADWEKAEQVTNCSSCPSSEDEAKATTDPVFQVVCKNEAKESPGKEFNTQETKETFVSPRTENNDYDTPKYFLSPGAAHRLKSQKCVAYKFRLVVKADGTQETNNGCRKVKIMPCSFPVSKDPSTKGLDPHLSHQMTKRKRMVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTINPICYALCNRTFRKTFKLLLLCRWKKKKVEEKLYWQGNSKLP

Target ORF information:

RefSeq Version	XM_006499099.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, muscarinic 5 (Chrm5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499099.4

ORF Insert Sequence:

ATGGAAGGGG AGTCTTATCA CAATGAAACC ACTGTCAACG GCACCCCAGT AAATCACCAG 
GCTTTGGAAC GCCATGGACT GTGGGAAGTC ATTACTATTG CAGCTGTGAC CGCTGTGGTC 
AGTCTGATGA CCATTGTCGG CAATGTCTTG GTCATGATCT CCTTCAAAGT CAACAGTCAG 
CTCAAGACAG TTAACAACTA TTACCTGCTC AGCTTGGCCT GTGCAGACCT CATCATTGGC 
ATCTTCTCCA TGAACCTCTA CACGACCTAC ATCCTCATGG GACGCTGGGT TCTCGGGAGT 
CTGGCCTGTG ACCTTTGGCT TGCACTCGAC TATGTAGCCA GCAACGCTTC TGTCATGAAC 
CTTCTGGTGA TTAGCTTTGA TCGTTACTTT TCCATCACAA GACCACTGAC ATACCGAGCC 
AAGCGTACCC CAAAGAGGGC TGGCATCATG ATCGGCTTGG CATGGCTGGT CTCCTTCATC 
CTCTGGGCGC CAGCCATCCT CTGCTGGCAG TACTTGGTCG GGAAGCGGAC AGTACCACCT 
GATGAGTGCC AGATCCAGTT CCTCTCTGAG CCCACCATCA CTTTTGGGAC CGCCATTGCC 
GCTTTCTATA TCCCTGTCTC CGTCATGACC ATACTCTACT GCCGGATCTA CCGGGAAACA 
GAGAAACGAA CCAAGGACCT GGCTGACCTC CAAGGTTCCG ATTCTGTGGC AGAAGTCAAG 
AAGAGAAAAC CGGCTCACAG GACCCTGCTC AGATCTTTCT TTAGCTGCCC TAGACCCAGC 
CTGGCCCAGA GAGTACGGAA CCAGGCCTCC TGGTCATCCT CCCGTAGAAG CACCTCAACA 
ACGGGAAAGC CAACCCAGGC CACTGATCTA AGTGCTGACT GGGAAAAGGC TGAGCAGGTT 
ACCAACTGTA GCAGCTGCCC CTCTTCAGAG GACGAAGCCA AGGCCACCAC TGACCCTGTC 
TTTCAAGTGG TCTGCAAGAA TGAGGCCAAG GAAAGCCCGG GGAAGGAATT CAATACCCAA 
GAGACCAAGG AAACGTTTGT GAGCCCTCGG ACTGAAAACA ATGACTATGA CACTCCCAAG 
TACTTCCTGT CTCCAGGTGC TGCTCACAGA CTCAAGAGTC AGAAGTGTGT TGCCTATAAG 
TTCCGATTGG TGGTAAAAGC TGACGGGACC CAGGAGACAA ACAATGGCTG TCGTAAGGTG 
AAAATCATGC CCTGTTCCTT CCCAGTGTCC AAAGACCCTT CAACAAAAGG CCTGGATCCC 
CACCTCAGCC ATCAAATGAC CAAACGAAAG AGAATGGTCC TAGTCAAAGA GAGGAAAGCG 
GCGCAGACCT TGAGCGCCAT TCTCCTGGCC TTCATCATCA CATGGACTCC TTATAACATC 
ATGGTCCTGG TTTCCACCTT CTGTGACAAG TGTGTCCCTG TCACCCTGTG GCACTTGGGT 
TACTGGCTGT GCTATGTCAA CAGCACCATC AACCCCATCT GTTACGCTCT CTGCAACAGA 
ACCTTCAGGA AGACCTTTAA GCTGCTGCTT CTCTGCCGGT GGAAAAAGAA AAAAGTAGAA 
GAGAAATTGT ATTGGCAAGG CAACAGCAAG CTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Differential regulation of primary afferent input to spinal cord by muscarinic receptor subtypes delineated using knockout mice.
The Journal of biological chemistry289(20)14321-30(2014 May)
Chen SR,Chen H,Yuan WX,Wess J,Pan HL

M5 receptor activation produces opposing physiological outcomes in dopamine neurons depending on the receptor's location.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(9)3253-62(2014 Feb)
Foster DJ,Gentry PR,Lizardi-Ortiz JE,Bridges TM,Wood MR,Niswender CM,Sulzer D,Lindsley CW,Xiang Z,Conn PJ

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Chrm5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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