Prepl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prepl
Entrez Gene ID	213760
Full Name	prolyl endopeptidase-like
Synonyms	2810457N15Rik,9530014L06Rik,D030028O16Rik,mKIAA0436
General protein information	Preferred Names prolyl endopeptidase-like Names prolyl endopeptidase-like
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163622.1	NP_001157094.1	prolyl endopeptidase-like isoform a
NM_145984.3	NP_666096.3	prolyl endopeptidase-like isoform b
NM_001163623.1	NP_001157095.1	prolyl endopeptidase-like isoform b
XM_006524001.3	XP_006524064.1	prolyl endopeptidase-like isoform X1
XM_006524001.4	XP_006524064.1	prolyl endopeptidase-like isoform X1
NM_001163624.1	NP_001157096.1	prolyl endopeptidase-like isoform b

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PREPL	XP_003586670.1
Gallus gallus (chicken)	PREPL	NP_001026224.1
Danio rerio (zebrafish)	prepl	NP_001139088.1
Pan troglodytes (chimpanzee)	PREPL	XP_003339066.1
	PREPL	XP_001112107.1
Rattus norvegicus (Norway rat)	Prepl	NP_001010951.1
Arabidopsis thaliana (thale cress)	AT1G69020	NP_177065.2
Oryza sativa (rice)	Os09g0475700	NP_001063461.1
Mus musculus (house mouse)	Prepl	NP_001157094.1
Xenopus tropicalis (tropical clawed frog)	prepl	XP_002932436.2
Homo sapiens (human)	PREPL	NP_006027.2
Canis lupus familiaris (dog)	PREPL	XP_005626131.1

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of PREPl causes growth impairment and hypotonia in mice.
Lone AM, Leidl M, McFedries AK, Horner JW, Creemers J, Saghatelian A
PloS one9(2)e89160(2014)

Cellular and ultra structural evidence for cytoskeletal localization of prolyl endopeptidase-like protein in neurons.
Morawski M, Nuytens K, Juhasz T, Zeitschel U, Seeger G, Waelkens E, Regal L, Schulz I, Arendt T, Szeltner Z, Creemers J, Rossner S
Neuroscience242128-39(2013 Jul)

Trans-Golgi network morphology and sorting is regulated by prolyl-oligopeptidase-like protein PREPL and the AP-1 complex subunit ?1A.
Radhakrishnan K, Baltes J, Creemers JW, Schu P
Journal of cell science126(Pt 5)1155-63(2013 Mar)

A conserved Pbx-Wnt-p63-Irf6 regulatory module controls face morphogenesis by promoting epithelial apoptosis.
Ferretti E, Li B, Zewdu R, Wells V, Hebert JM, Karner C, Anderson MJ, Williams T, Dixon J, Dixon MJ, Depew MJ, Selleri L
Developmental cell21(4)627-41(2011 Oct)

A substrate-free activity-based protein profiling screen for the discovery of selective PREPL inhibitors.
Lone AM, Bachovchin DA, Westwood DB, Speers AE, Spicer TP, Fernandez-Vega V, Chase P, Hodder PS, Rosen H, Cravatt BF, Saghatelian A
Journal of the American Chemical Society133(30)11665-74(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data demonstrate PREPL as an AP-1 effector that takes part in the regulation of AP-1 membrane binding. PREPL is highly expressed in brain and at lower levels in muscle and kidney.
Title: Trans-Golgi network morphology and sorting is regulated by prolyl-oligopeptidase-like protein PREPL and the AP-1 complex subunit ?1A.

The following Prepl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prepl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22451	NM_001163623.1 Latest version!	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu22451	NM_001163624.1 Latest version!	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu22451	NM_145984.3 Latest version!	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu22531	NM_001163622.1 Latest version!	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OMu22451	XM_006524001.4 Latest version!	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu22451	XM_006524001.3	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prepl ( NM_145984.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666096.3 Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22451
Clone ID Related Accession (Same CDS sequence)	NM_145984.3 , NM_001163623.1 , XM_006524001.3 , XM_006524001.4 , NM_001163624.1
Accession Version	NM_145984.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase-like isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY146774.1, AK083070.1, AK050878.1 and BU058006.1. On Jul 31, 2009 this sequence version replaced NM_145984.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Variants 1, 2 and 3 encode the same isoform (b). ##Evidence-Data-START## Transcript exon combination :: AK083070.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666096.3
CDS	311..2227
Translation	MDAFEKVRTRLETQPQEEYEVVNAEIKHGGFVYYQEGCCLVRSKDEEADSDNYEVLFNLEELKLDQPFIDCIRVAPDEKYVAAKIRTEDSETSTLVVVKLSDQPVMEASFPNVSSFEWVKDEEDEDVLFYTFQRNLRCHDVYRATFGDNKRNERFYTEKDPSYFVFLYLTKDSRFLTLNIMNKTTSEVWLIDGLSPWDPPVLIQKRIHGMLYYVEHRDDELYILTNVGEPTEFKLMRTAADAPAIMNWDLFFTMKRNTKVVDLDMFKDHCVLFLKHSNLLYVNVIGLADDSVRSLKLPPWACGFIMDTNSDPKNCPFQLCSPIRPPKYYTYKFAEGKLFEETGHEDPITKTSRVLRIEAKSKDGKLVPMTVFHKTDSEDLQRKPLLVHVYGAYGMDLKMNFRPEKRVLVDDGWILAYCHVRGGGELGLQWHADGRLTKKLNGLADLVACIKTLHSQGFSQPSLTTLSAFSAGGVLVGALCNSKPELLRAVTLEAPFLDVLNTMLDTTLPLTLEELEEWGNPSSDEKHKNYIKRYCPCQNIKPQHYPSVHITAYENDERVPLKGIVNYTEKLKEAVAEHTKGAGEGYQPPNIILDIQPGGNHVIEDSHKKITTQMKFLYEELGLDSTDAFEALKKYLKF

Target ORF information:

RefSeq Version	NM_145984.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145984.3

ORF Insert Sequence:

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prepl ( NM_001163623.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157095.1 Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu22451
Clone ID Related Accession (Same CDS sequence)	NM_145984.3 , NM_001163623.1 , XM_006524001.3 , XM_006524001.4 , NM_001163624.1
Accession Version	NM_001163623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase-like isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY146774.1, AK083070.1, AK050878.1 and BU058006.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Variants 1, 2 and 3 encode the same isoform (b). ##Evidence-Data-START## Transcript exon combination :: BC004612.1, SRR1660815.150125.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157095.1
CDS	211..2127
Translation	MDAFEKVRTRLETQPQEEYEVVNAEIKHGGFVYYQEGCCLVRSKDEEADSDNYEVLFNLEELKLDQPFIDCIRVAPDEKYVAAKIRTEDSETSTLVVVKLSDQPVMEASFPNVSSFEWVKDEEDEDVLFYTFQRNLRCHDVYRATFGDNKRNERFYTEKDPSYFVFLYLTKDSRFLTLNIMNKTTSEVWLIDGLSPWDPPVLIQKRIHGMLYYVEHRDDELYILTNVGEPTEFKLMRTAADAPAIMNWDLFFTMKRNTKVVDLDMFKDHCVLFLKHSNLLYVNVIGLADDSVRSLKLPPWACGFIMDTNSDPKNCPFQLCSPIRPPKYYTYKFAEGKLFEETGHEDPITKTSRVLRIEAKSKDGKLVPMTVFHKTDSEDLQRKPLLVHVYGAYGMDLKMNFRPEKRVLVDDGWILAYCHVRGGGELGLQWHADGRLTKKLNGLADLVACIKTLHSQGFSQPSLTTLSAFSAGGVLVGALCNSKPELLRAVTLEAPFLDVLNTMLDTTLPLTLEELEEWGNPSSDEKHKNYIKRYCPCQNIKPQHYPSVHITAYENDERVPLKGIVNYTEKLKEAVAEHTKGAGEGYQPPNIILDIQPGGNHVIEDSHKKITTQMKFLYEELGLDSTDAFEALKKYLKF

Target ORF information:

RefSeq Version	NM_001163623.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163623.1

ORF Insert Sequence:

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prepl ( NM_001163624.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157096.1 Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu22451
Clone ID Related Accession (Same CDS sequence)	NM_145984.3 , NM_001163623.1 , XM_006524001.3 , XM_006524001.4 , NM_001163624.1
Accession Version	NM_001163624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase-like isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY257815.1, AK050878.1, AK083070.1 and BU058006.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Variants 1, 2 and 3 encode the same isoform (b). ##Evidence-Data-START## Transcript exon combination :: SRR1660811.116858.1, SRR1660817.288573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157096.1
CDS	79..1995
Translation	MDAFEKVRTRLETQPQEEYEVVNAEIKHGGFVYYQEGCCLVRSKDEEADSDNYEVLFNLEELKLDQPFIDCIRVAPDEKYVAAKIRTEDSETSTLVVVKLSDQPVMEASFPNVSSFEWVKDEEDEDVLFYTFQRNLRCHDVYRATFGDNKRNERFYTEKDPSYFVFLYLTKDSRFLTLNIMNKTTSEVWLIDGLSPWDPPVLIQKRIHGMLYYVEHRDDELYILTNVGEPTEFKLMRTAADAPAIMNWDLFFTMKRNTKVVDLDMFKDHCVLFLKHSNLLYVNVIGLADDSVRSLKLPPWACGFIMDTNSDPKNCPFQLCSPIRPPKYYTYKFAEGKLFEETGHEDPITKTSRVLRIEAKSKDGKLVPMTVFHKTDSEDLQRKPLLVHVYGAYGMDLKMNFRPEKRVLVDDGWILAYCHVRGGGELGLQWHADGRLTKKLNGLADLVACIKTLHSQGFSQPSLTTLSAFSAGGVLVGALCNSKPELLRAVTLEAPFLDVLNTMLDTTLPLTLEELEEWGNPSSDEKHKNYIKRYCPCQNIKPQHYPSVHITAYENDERVPLKGIVNYTEKLKEAVAEHTKGAGEGYQPPNIILDIQPGGNHVIEDSHKKITTQMKFLYEELGLDSTDAFEALKKYLKF

Target ORF information:

RefSeq Version	NM_001163624.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163624.1

ORF Insert Sequence:

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prepl ( XM_006524001.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524064.1 Mus musculus prolyl endopeptidase-like (Prepl), transcript variant X1, mRNA.

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CloneID	OMu22451
Clone ID Related Accession (Same CDS sequence)	NM_145984.3 , NM_001163623.1 , XM_006524001.3 , XM_006524001.4 , NM_001163624.1
Accession Version	XM_006524001.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524064.1
CDS	306..2222
Translation	MDAFEKVRTRLETQPQEEYEVVNAEIKHGGFVYYQEGCCLVRSKDEEADSDNYEVLFNLEELKLDQPFIDCIRVAPDEKYVAAKIRTEDSETSTLVVVKLSDQPVMEASFPNVSSFEWVKDEEDEDVLFYTFQRNLRCHDVYRATFGDNKRNERFYTEKDPSYFVFLYLTKDSRFLTLNIMNKTTSEVWLIDGLSPWDPPVLIQKRIHGMLYYVEHRDDELYILTNVGEPTEFKLMRTAADAPAIMNWDLFFTMKRNTKVVDLDMFKDHCVLFLKHSNLLYVNVIGLADDSVRSLKLPPWACGFIMDTNSDPKNCPFQLCSPIRPPKYYTYKFAEGKLFEETGHEDPITKTSRVLRIEAKSKDGKLVPMTVFHKTDSEDLQRKPLLVHVYGAYGMDLKMNFRPEKRVLVDDGWILAYCHVRGGGELGLQWHADGRLTKKLNGLADLVACIKTLHSQGFSQPSLTTLSAFSAGGVLVGALCNSKPELLRAVTLEAPFLDVLNTMLDTTLPLTLEELEEWGNPSSDEKHKNYIKRYCPCQNIKPQHYPSVHITAYENDERVPLKGIVNYTEKLKEAVAEHTKGAGEGYQPPNIILDIQPGGNHVIEDSHKKITTQMKFLYEELGLDSTDAFEALKKYLKF

Target ORF information:

RefSeq Version	XM_006524001.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524001.3

ORF Insert Sequence:

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prepl ( NM_001163622.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157094.1 Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 1, mRNA.

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CloneID	OMu22531
Clone ID Related Accession (Same CDS sequence)	NM_001163622.1
Accession Version	NM_001163622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase-like isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY146774.1, AK028877.1, AK083070.1 and BU058006.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660817.284654.1, SRR1660815.134040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGA CGGCCAAATT CTCTCTCCGA GCTTTGAAGC ATAGTATTCC TCATCTTGGG 
AACTGCATGC AGAAACAGAG TTACCGTAAC GTCGCTGGTC CTTATTACAG TAGAGTAAGA 
TTGAAAAAGT ACCACCTAAC CAAGTGTCTG CAGAATAAAC CCAGGATAGC AGGATTAGCA 
AGAAACATCC CAAGTCGGAG CTTCTCGTGT AAGGATCTTC TGCCTATTAA GCCAGAAAGT 
GAAAAGCCCA TTTCTGAAAA CATGGATGCA TTTGAAAAAG TGAGAACAAG ACTGGAAACA 
CAGCCACAGG AAGAGTACGA AGTCGTCAAT GCAGAGATTA AACATGGCGG CTTTGTTTAT 
TATCAAGAAG GTTGCTGCTT GGTTCGTTCC AAAGACGAAG AAGCTGACAG TGATAATTAT 
GAAGTTTTAT TCAACCTGGA AGAACTTAAA TTGGATCAGC CTTTCATTGA TTGCATCAGA 
GTTGCTCCTG ATGAGAAATA CGTGGCTGCC AAGATAAGGA CGGAGGATTC TGAAACCTCC 
ACCTTAGTAG TTGTGAAGCT CAGTGACCAG CCTGTAATGG AAGCGTCGTT CCCAAACGTG 
TCCAGTTTCG AATGGGTGAA GGATGAAGAA GATGAAGATG TCTTATTCTA CACCTTCCAA 
AGGAACCTTC GCTGCCACGA TGTGTATCGA GCCACATTTG GTGACAACAA GCGCAATGAA 
CGGTTCTACA CAGAGAAAGA CCCAAGCTAT TTTGTCTTCC TCTACCTTAC AAAAGACAGT 
CGTTTCCTCA CACTGAATAT TATGAACAAG ACGACTTCCG AGGTGTGGTT GATAGATGGC 
CTGAGCCCGT GGGACCCACC AGTCCTTATT CAGAAGAGGA TACACGGAAT GCTTTACTAT 
GTTGAGCATC GAGATGATGA GTTATACATC CTGACGAATG TTGGAGAACC TACAGAATTC 
AAGCTGATGC GGACGGCAGC TGATGCCCCT GCTATTATGA ATTGGGATTT ATTTTTCACA 
ATGAAGAGAA ATACCAAGGT TGTAGACTTG GACATGTTTA AGGACCACTG TGTTCTGTTC 
CTGAAACACA GCAATCTCCT CTATGTTAAT GTGATTGGTC TGGCCGACGA CTCAGTGCGC 
TCCCTAAAGC TCCCGCCTTG GGCCTGTGGA TTCATAATGG ATACAAATTC TGACCCAAAG 
AACTGCCCCT TTCAGCTTTG CTCTCCAATA AGACCCCCAA AATATTACAC ATACAAATTT 
GCAGAAGGCA AACTGTTTGA GGAAACTGGT CACGAAGATC CGATTACAAA GACCAGCCGT 
GTTTTACGCA TAGAAGCCAA AAGCAAGGAT GGAAAGTTAG TGCCCATGAC AGTTTTTCAC 
AAAACCGATT CTGAGGACCT GCAGAGGAAG CCTCTCTTGG TGCACGTCTA TGGAGCGTAC 
GGAATGGACC TGAAAATGAA TTTCAGGCCT GAAAAGCGGG TCTTGGTGGA TGATGGATGG 
ATCTTAGCTT ATTGCCATGT TAGGGGTGGT GGCGAGCTAG GTCTCCAGTG GCATGCCGAT 
GGCCGACTAA CTAAAAAGCT CAATGGCCTT GCTGACTTAG TGGCTTGCAT TAAGACGCTT 
CACAGCCAGG GCTTTTCTCA GCCGAGCCTA ACAACGCTGA GCGCTTTCAG TGCTGGAGGT 
GTGCTCGTGG GAGCACTGTG TAATTCTAAG CCAGAGCTCC TGAGAGCCGT GACCCTGGAG 
GCACCTTTCT TGGATGTCCT CAATACCATG TTGGATACCA CCCTTCCTCT GACACTGGAA 
GAGTTGGAAG AGTGGGGGAA CCCGTCATCT GATGAGAAAC ACAAGAACTA CATAAAGCGC 
TACTGTCCCT GCCAGAACAT TAAGCCTCAG CATTATCCTT CAGTTCACAT CACAGCCTAT 
GAAAACGATG AGCGAGTGCC GCTGAAAGGA ATCGTGAACT ACACAGAGAA GCTTAAGGAG 
GCCGTGGCTG AGCACACCAA GGGAGCGGGT GAAGGCTATC AGCCCCCCAA CATTATCCTA 
GATATTCAGC CTGGGGGGAA CCACGTGATT GAGGATTCTC ACAAAAAGAT CACAACCCAG 
ATGAAATTCC TATATGAGGA ACTTGGACTT GACAGCACTG ATGCTTTCGA GGCGCTGAAG 
AAATACCTAA AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157094.1
CDS	486..2663
Translation	MLQTAKFSLRALKHSIPHLGNCMQKQSYRNVAGPYYSRVRLKKYHLTKCLQNKPRIAGLARNIPSRSFSCKDLLPIKPESEKPISENMDAFEKVRTRLETQPQEEYEVVNAEIKHGGFVYYQEGCCLVRSKDEEADSDNYEVLFNLEELKLDQPFIDCIRVAPDEKYVAAKIRTEDSETSTLVVVKLSDQPVMEASFPNVSSFEWVKDEEDEDVLFYTFQRNLRCHDVYRATFGDNKRNERFYTEKDPSYFVFLYLTKDSRFLTLNIMNKTTSEVWLIDGLSPWDPPVLIQKRIHGMLYYVEHRDDELYILTNVGEPTEFKLMRTAADAPAIMNWDLFFTMKRNTKVVDLDMFKDHCVLFLKHSNLLYVNVIGLADDSVRSLKLPPWACGFIMDTNSDPKNCPFQLCSPIRPPKYYTYKFAEGKLFEETGHEDPITKTSRVLRIEAKSKDGKLVPMTVFHKTDSEDLQRKPLLVHVYGAYGMDLKMNFRPEKRVLVDDGWILAYCHVRGGGELGLQWHADGRLTKKLNGLADLVACIKTLHSQGFSQPSLTTLSAFSAGGVLVGALCNSKPELLRAVTLEAPFLDVLNTMLDTTLPLTLEELEEWGNPSSDEKHKNYIKRYCPCQNIKPQHYPSVHITAYENDERVPLKGIVNYTEKLKEAVAEHTKGAGEGYQPPNIILDIQPGGNHVIEDSHKKITTQMKFLYEELGLDSTDAFEALKKYLKF

Target ORF information:

RefSeq Version	NM_001163622.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163622.1

ORF Insert Sequence:

ATGCTGCAGA CGGCCAAATT CTCTCTCCGA GCTTTGAAGC ATAGTATTCC TCATCTTGGG 
AACTGCATGC AGAAACAGAG TTACCGTAAC GTCGCTGGTC CTTATTACAG TAGAGTAAGA 
TTGAAAAAGT ACCACCTAAC CAAGTGTCTG CAGAATAAAC CCAGGATAGC AGGATTAGCA 
AGAAACATCC CAAGTCGGAG CTTCTCGTGT AAGGATCTTC TGCCTATTAA GCCAGAAAGT 
GAAAAGCCCA TTTCTGAAAA CATGGATGCA TTTGAAAAAG TGAGAACAAG ACTGGAAACA 
CAGCCACAGG AAGAGTACGA AGTCGTCAAT GCAGAGATTA AACATGGCGG CTTTGTTTAT 
TATCAAGAAG GTTGCTGCTT GGTTCGTTCC AAAGACGAAG AAGCTGACAG TGATAATTAT 
GAAGTTTTAT TCAACCTGGA AGAACTTAAA TTGGATCAGC CTTTCATTGA TTGCATCAGA 
GTTGCTCCTG ATGAGAAATA CGTGGCTGCC AAGATAAGGA CGGAGGATTC TGAAACCTCC 
ACCTTAGTAG TTGTGAAGCT CAGTGACCAG CCTGTAATGG AAGCGTCGTT CCCAAACGTG 
TCCAGTTTCG AATGGGTGAA GGATGAAGAA GATGAAGATG TCTTATTCTA CACCTTCCAA 
AGGAACCTTC GCTGCCACGA TGTGTATCGA GCCACATTTG GTGACAACAA GCGCAATGAA 
CGGTTCTACA CAGAGAAAGA CCCAAGCTAT TTTGTCTTCC TCTACCTTAC AAAAGACAGT 
CGTTTCCTCA CACTGAATAT TATGAACAAG ACGACTTCCG AGGTGTGGTT GATAGATGGC 
CTGAGCCCGT GGGACCCACC AGTCCTTATT CAGAAGAGGA TACACGGAAT GCTTTACTAT 
GTTGAGCATC GAGATGATGA GTTATACATC CTGACGAATG TTGGAGAACC TACAGAATTC 
AAGCTGATGC GGACGGCAGC TGATGCCCCT GCTATTATGA ATTGGGATTT ATTTTTCACA 
ATGAAGAGAA ATACCAAGGT TGTAGACTTG GACATGTTTA AGGACCACTG TGTTCTGTTC 
CTGAAACACA GCAATCTCCT CTATGTTAAT GTGATTGGTC TGGCCGACGA CTCAGTGCGC 
TCCCTAAAGC TCCCGCCTTG GGCCTGTGGA TTCATAATGG ATACAAATTC TGACCCAAAG 
AACTGCCCCT TTCAGCTTTG CTCTCCAATA AGACCCCCAA AATATTACAC ATACAAATTT 
GCAGAAGGCA AACTGTTTGA GGAAACTGGT CACGAAGATC CGATTACAAA GACCAGCCGT 
GTTTTACGCA TAGAAGCCAA AAGCAAGGAT GGAAAGTTAG TGCCCATGAC AGTTTTTCAC 
AAAACCGATT CTGAGGACCT GCAGAGGAAG CCTCTCTTGG TGCACGTCTA TGGAGCGTAC 
GGAATGGACC TGAAAATGAA TTTCAGGCCT GAAAAGCGGG TCTTGGTGGA TGATGGATGG 
ATCTTAGCTT ATTGCCATGT TAGGGGTGGT GGCGAGCTAG GTCTCCAGTG GCATGCCGAT 
GGCCGACTAA CTAAAAAGCT CAATGGCCTT GCTGACTTAG TGGCTTGCAT TAAGACGCTT 
CACAGCCAGG GCTTTTCTCA GCCGAGCCTA ACAACGCTGA GCGCTTTCAG TGCTGGAGGT 
GTGCTCGTGG GAGCACTGTG TAATTCTAAG CCAGAGCTCC TGAGAGCCGT GACCCTGGAG 
GCACCTTTCT TGGATGTCCT CAATACCATG TTGGATACCA CCCTTCCTCT GACACTGGAA 
GAGTTGGAAG AGTGGGGGAA CCCGTCATCT GATGAGAAAC ACAAGAACTA CATAAAGCGC 
TACTGTCCCT GCCAGAACAT TAAGCCTCAG CATTATCCTT CAGTTCACAT CACAGCCTAT 
GAAAACGATG AGCGAGTGCC GCTGAAAGGA ATCGTGAACT ACACAGAGAA GCTTAAGGAG 
GCCGTGGCTG AGCACACCAA GGGAGCGGGT GAAGGCTATC AGCCCCCCAA CATTATCCTA 
GATATTCAGC CTGGGGGGAA CCACGTGATT GAGGATTCTC ACAAAAAGAT CACAACCCAG 
ATGAAATTCC TATATGAGGA ACTTGGACTT GACAGCACTG ATGCTTTCGA GGCGCTGAAG 
AAATACCTAA AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prepl ( XM_006524001.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524064.1 Mus musculus prolyl endopeptidase-like (Prepl), transcript variant X1, mRNA.

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CloneID	OMu22451
Clone ID Related Accession (Same CDS sequence)	NM_145984.3 , NM_001163623.1 , XM_006524001.3 , XM_006524001.4 , NM_001163624.1
Accession Version	XM_006524001.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524001.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524064.1
CDS	551..2467
Translation	MDAFEKVRTRLETQPQEEYEVVNAEIKHGGFVYYQEGCCLVRSKDEEADSDNYEVLFNLEELKLDQPFIDCIRVAPDEKYVAAKIRTEDSETSTLVVVKLSDQPVMEASFPNVSSFEWVKDEEDEDVLFYTFQRNLRCHDVYRATFGDNKRNERFYTEKDPSYFVFLYLTKDSRFLTLNIMNKTTSEVWLIDGLSPWDPPVLIQKRIHGMLYYVEHRDDELYILTNVGEPTEFKLMRTAADAPAIMNWDLFFTMKRNTKVVDLDMFKDHCVLFLKHSNLLYVNVIGLADDSVRSLKLPPWACGFIMDTNSDPKNCPFQLCSPIRPPKYYTYKFAEGKLFEETGHEDPITKTSRVLRIEAKSKDGKLVPMTVFHKTDSEDLQRKPLLVHVYGAYGMDLKMNFRPEKRVLVDDGWILAYCHVRGGGELGLQWHADGRLTKKLNGLADLVACIKTLHSQGFSQPSLTTLSAFSAGGVLVGALCNSKPELLRAVTLEAPFLDVLNTMLDTTLPLTLEELEEWGNPSSDEKHKNYIKRYCPCQNIKPQHYPSVHITAYENDERVPLKGIVNYTEKLKEAVAEHTKGAGEGYQPPNIILDIQPGGNHVIEDSHKKITTQMKFLYEELGLDSTDAFEALKKYLKF

Target ORF information:

RefSeq Version	XM_006524001.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl endopeptidase-like (Prepl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524001.4

ORF Insert Sequence:

ATGGATGCAT TTGAAAAAGT GAGAACAAGA CTGGAAACAC AGCCACAGGA AGAGTACGAA 
GTCGTCAATG CAGAGATTAA ACATGGCGGC TTTGTTTATT ATCAAGAAGG TTGCTGCTTG 
GTTCGTTCCA AAGACGAAGA AGCTGACAGT GATAATTATG AAGTTTTATT CAACCTGGAA 
GAACTTAAAT TGGATCAGCC TTTCATTGAT TGCATCAGAG TTGCTCCTGA TGAGAAATAC 
GTGGCTGCCA AGATAAGGAC GGAGGATTCT GAAACCTCCA CCTTAGTAGT TGTGAAGCTC 
AGTGACCAGC CTGTAATGGA AGCGTCGTTC CCAAACGTGT CCAGTTTCGA ATGGGTGAAG 
GATGAAGAAG ATGAAGATGT CTTATTCTAC ACCTTCCAAA GGAACCTTCG CTGCCACGAT 
GTGTATCGAG CCACATTTGG TGACAACAAG CGCAATGAAC GGTTCTACAC AGAGAAAGAC 
CCAAGCTATT TTGTCTTCCT CTACCTTACA AAAGACAGTC GTTTCCTCAC ACTGAATATT 
ATGAACAAGA CGACTTCCGA GGTGTGGTTG ATAGATGGCC TGAGCCCGTG GGACCCACCA 
GTCCTTATTC AGAAGAGGAT ACACGGAATG CTTTACTATG TTGAGCATCG AGATGATGAG 
TTATACATCC TGACGAATGT TGGAGAACCT ACAGAATTCA AGCTGATGCG GACGGCAGCT 
GATGCCCCTG CTATTATGAA TTGGGATTTA TTTTTCACAA TGAAGAGAAA TACCAAGGTT 
GTAGACTTGG ACATGTTTAA GGACCACTGT GTTCTGTTCC TGAAACACAG CAATCTCCTC 
TATGTTAATG TGATTGGTCT GGCCGACGAC TCAGTGCGCT CCCTAAAGCT CCCGCCTTGG 
GCCTGTGGAT TCATAATGGA TACAAATTCT GACCCAAAGA ACTGCCCCTT TCAGCTTTGC 
TCTCCAATAA GACCCCCAAA ATATTACACA TACAAATTTG CAGAAGGCAA ACTGTTTGAG 
GAAACTGGTC ACGAAGATCC GATTACAAAG ACCAGCCGTG TTTTACGCAT AGAAGCCAAA 
AGCAAGGATG GAAAGTTAGT GCCCATGACA GTTTTTCACA AAACCGATTC TGAGGACCTG 
CAGAGGAAGC CTCTCTTGGT GCACGTCTAT GGAGCGTACG GAATGGACCT GAAAATGAAT 
TTCAGGCCTG AAAAGCGGGT CTTGGTGGAT GATGGATGGA TCTTAGCTTA TTGCCATGTT 
AGGGGTGGTG GCGAGCTAGG TCTCCAGTGG CATGCCGATG GCCGACTAAC TAAAAAGCTC 
AATGGCCTTG CTGACTTAGT GGCTTGCATT AAGACGCTTC ACAGCCAGGG CTTTTCTCAG 
CCGAGCCTAA CAACGCTGAG CGCTTTCAGT GCTGGAGGTG TGCTCGTGGG AGCACTGTGT 
AATTCTAAGC CAGAGCTCCT GAGAGCCGTG ACCCTGGAGG CACCTTTCTT GGATGTCCTC 
AATACCATGT TGGATACCAC CCTTCCTCTG ACACTGGAAG AGTTGGAAGA GTGGGGGAAC 
CCGTCATCTG ATGAGAAACA CAAGAACTAC ATAAAGCGCT ACTGTCCCTG CCAGAACATT 
AAGCCTCAGC ATTATCCTTC AGTTCACATC ACAGCCTATG AAAACGATGA GCGAGTGCCG 
CTGAAAGGAA TCGTGAACTA CACAGAGAAG CTTAAGGAGG CCGTGGCTGA GCACACCAAG 
GGAGCGGGTG AAGGCTATCA GCCCCCCAAC ATTATCCTAG ATATTCAGCC TGGGGGGAAC 
CACGTGATTG AGGATTCTCA CAAAAAGATC ACAACCCAGA TGAAATTCCT ATATGAGGAA 
CTTGGACTTG ACAGCACTGA TGCTTTCGAG GCGCTGAAGA AATACCTAAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of PREPl causes growth impairment and hypotonia in mice.
PloS one9(2)e89160(2014)
Lone AM,Leidl M,McFedries AK,Horner JW,Creemers J,Saghatelian A

Cellular and ultra structural evidence for cytoskeletal localization of prolyl endopeptidase-like protein in neurons.
Neuroscience242128-39(2013 Jul)
Morawski M,Nuytens K,Juhasz T,Zeitschel U,Seeger G,Waelkens E,Regal L,Schulz I,Arendt T,Szeltner Z,Creemers J,Rossner S

Trans-Golgi network morphology and sorting is regulated by prolyl-oligopeptidase-like protein PREPL and the AP-1 complex subunit ?1A.
Journal of cell science126(Pt 5)1155-63(2013 Mar)
Radhakrishnan K,Baltes J,Creemers JW,Schu P

A conserved Pbx-Wnt-p63-Irf6 regulatory module controls face morphogenesis by promoting epithelial apoptosis.
Developmental cell21(4)627-41(2011 Oct)
Ferretti E,Li B,Zewdu R,Wells V,Hebert JM,Karner C,Anderson MJ,Williams T,Dixon J,Dixon MJ,Depew MJ,Selleri L

A substrate-free activity-based protein profiling screen for the discovery of selective PREPL inhibitors.
Journal of the American Chemical Society133(30)11665-74(2011 Aug)
Lone AM,Bachovchin DA,Westwood DB,Speers AE,Spicer TP,Fernandez-Vega V,Chase P,Hodder PS,Rosen H,Cravatt BF,Saghatelian A

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Prepl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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