Syne3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Syne3
Entrez Gene ID	212073
Full Name	spectrin repeat containing, nuclear envelope family member 3
Synonyms	KASH3,nesprin-3
General protein information	Preferred Names spectrin repeat containing, nuclear envelope family member 3 Names nesprin-3 KASH domain-containing protein 3 nuclear envelope spectrin repeat protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 E

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515653.2	XP_006515716.1	nesprin-3 isoform X1
XM_006515652.4	XP_006515715.3	nesprin-3 isoform X1
XM_006515654.4	XP_006515717.1	nesprin-3 isoform X2
NM_001042699.2	NP_001036164.1	nesprin-3 isoform alpha
NM_172500.3	NP_766088.2	nesprin-3 isoform beta
XM_006515652.3	XP_006515715.2	nesprin-3 isoform X1
XM_006515654.3	XP_006515717.1	nesprin-3 isoform X1
XM_006515653.3	XP_006515716.1	nesprin-3 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SYNE3	NP_689805.3
Bos taurus (cattle)	C21H14orf49	NP_001192798.1
Canis lupus familiaris (dog)	SYNE3	XP_005623830.1
Gallus gallus (chicken)	C5H14ORF49	XP_004941962.1
	LOC710721	XP_001099387.1
Mus musculus (house mouse)	Syne3	NP_001036164.1
Rattus norvegicus (Norway rat)	Syne3	NP_001100232.1
Danio rerio (zebrafish)	syne3	NP_001076483.2
Xenopus tropicalis (tropical clawed frog)	syne3	XP_004917142.1

Gene Ontology
Bibliography

Related articles in PubMed

p63 Transcription Factor Regulates Nuclear?Shape and Expression of Nuclear?Envelope-Associated Genes in Epidermal Keratinocytes.
Rapisarda V, Malashchuk I, Asamaowei IE, Poterlowicz K, Fessing MY, Sharov AA, Karakesisoglou I, Botchkarev VA, Mardaryev A
The Journal of investigative dermatology137(10)2157-2167(2017 Oct)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Nesprin-3 connects plectin and vimentin to the nuclear envelope of Sertoli cells but is not required for Sertoli cell function in spermatogenesis.
Ketema M, Kreft M, Secades P, Janssen H, Sonnenberg A
Molecular biology of the cell24(15)2454-66(2013 Aug)

The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction.
Chambliss AB, Khatau SB, Erdenberger N, Robinson DK, Hodzic D, Longmore GD, Wirtz D
Scientific reports31087(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nesprin-3 is critical for the localization of plectin to the nuclear perimeter of Sertoli cells.
Title: Nesprin-3 connects plectin and vimentin to the nuclear envelope of Sertoli cells but is not required for Sertoli cell function in spermatogenesis.

Data indicate roles for linkers of nucleus to cytoskeleton (LINC) molecules nesprin2giant and nesprin3, which anchor actin cap fibers to the nucleus.
Title: The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction.

Mouse nesprin-3alpha co-immunoprecipitated with torsinA and this involved the C-terminal region of torsinA and the KASH domain of nesprin-3alpha.
Title: TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton.

Data support a model in which the actin cytoskeleton influences the binding of plectin dimers to dimers of nesprin-3alpha, which in turn are retained at the nuclear envelope through an interaction with Sun proteins.
Title: Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin.

plectin binds to the integrin alpha6beta4 at the cell surface and to nesprin-3 at the ONM in keratinocytes.
Title: Nesprin-3, a novel outer nuclear membrane protein, associates with the cytoskeletal linker protein plectin.

The following Syne3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Syne3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13761	NM_172500.3 Latest version!	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$524.30 $749.00
OMu14134	NM_001042699.2 Latest version!	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu14134	XM_006515652.3 Latest version!	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu14134	XM_006515653.3 Latest version!	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu14134	XM_006515654.4 Latest version!	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu86023	XM_006515652.4 Latest version!	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu14134	XM_006515654.3	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu14134	XM_006515653.2	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Syne3 ( NM_172500.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766088.2 Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13761
Clone ID Related Accession (Same CDS sequence)	NM_172500.3
Accession Version	NM_172500.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	nesprin-3 isoform beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029216.1, AK030542.1, BB733613.1 and AC147375.3. On Nov 6, 2013 this sequence version replaced NM_172500.2. Transcript Variant: This variant (2) uses an alternate 5'-terminal exon, lacks several exons in the 5' and central coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (beta) has a distinct and shorter N-terminus, compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029216.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGACAACAC GAAGGGGCCC CGAGCGGCCC TGGAGGCAAG GCTTCGAGAG ACAGAGTCGC 
ATCGAGTGGG TGTGGCTGCA CTGGAGTGAG TACCTGCTGG CCCAGGATGA GTTTTACCGC 
TGGTTCCAGA AGATGGTGGT CGCACTGGAG CCCCCCGTGG AGCTGCAGCT GGGCTTGAAG 
GAGAAGCAAT GGCAGCTGAG CCACGCCCAG GTGCTGCTGC ACAACGTGGA CAATCAGGCT 
GTGCTCCTGG ACAGGCTGTT GGAGGAGGCG GGCTCCCTGT TCAGCAGGAT CGGAGACCCC 
AGCGTGGATG AAGATGCCCA GAAGAGGATG AAGGCTGAGT ACGATGCCGT GAAGGCCAGA 
GCCCAGCGCA GGGTGGACCT CCTGGCCCAG GTGGCCCAGG ACCATGAGCA GTACCGGGAG 
GACGTGAATG AGTTCCAGCT GTGGCTGAAG GCGGTGGTGG AGAAGGTGCA CAGCTGTCTG 
GGGCGGAACT GCAAGCTGGC CACAGAACTT CGTCTCTCTA CGCTGCAGGA CATCGCCAAG 
GATTTCCCTA GGGGTGAGGA GTCTCTGAAA AGATTGGAGG AACAGGCTGT GGGTGTCATT 
CAAAACACCT CTCCCTTGGG TGCAGAGAAG ATCTCAGGGG AGCTGGAGGA GATGCGGGGT 
GTCCTGGAGA AGCTGAGAGT CCTCTGGAAA GAGGAGGAAG GGAGGCTGCG GGGCCTGCTC 
CAGTCCAGGG GGGACTGTGA GCAGCAGATC CAACAGCTGG AGGCAGAACT GGGAGACTTC 
AAGAAAAGCC TTCAGAGGCT GGCCCAGGAG GGCTTGGAGC CCACGGTGAA GACAGCCACA 
GAGGATGAGC TGGTGGCCCA GTGGAGGCTG TTCTCGGGGA CTCGGGCTGC ACTGGCTTCA 
GAGGAACCCC GTGTAGACCG GTTACAAACT CAACTGAAGA AACTTGTCAC CTTCCCGGAC 
CTGCAGTCAC TCTCTGACAG CGTGGTAGCC ACCATTCAGG AATACCAAAG TATGAAGGGG 
AAGAATACCA GGCTCCACAA TGCGACCCGG GCAGAGCTGT GGCAGCGTTT CCAGCGGCCC 
CTAAATGACC TGCAGCTGTG GAAGGCCCTG GCCCAGAGGC TCCTGGACAT CACGGCCAGC 
CTGCCTGACC TGGCCTCCAT TCACACCTTT CTACCCCAGA TTGAGGCGGC CCTCACGGAA 
AGCTCTCGCC TGAAAGAGCA GCTGGCGATG CTGCAGCTGA AGACCGACCT GCTGGGCAGC 
ATCTTTGGCC AGGAGAGAGC AGCCACCCTC CTGGAGCAGG TGACAAGTTC TGTGAGGGAC 
AGAGACCTAC TGCATAACAG CCTTCTTCAG CGGAAGAGCA AACTTCAGAG CCTGCTTGTT 
CAGCACAAGG ACTTTGGGGT GGCTTTTGAT CCCCTAAACA GGAAGCTCCT AGACCTCCAG 
GCCAGGATCC AAGCAGAGAA AGGGCTTCCG AGGGACCTTC CTGGAAAGCA GGTCCAGCTC 
CTAAGGTTGC AGGGGCTGCA GGAAGAGGGG CTGGATCTGG GGACACAGAT CGAGGCTGTG 
AGGCCTCTTG CCCATGGGAA CTCTAAGCAC CAGCAGAAAG TAGACCAGAT CTCCTGTGAC 
CAGCAAGCCC TGCAGAGGTC CCTGGAGGAT CTCGTGGACA GGTGTCAGCA GAACGTACGG 
GAACATTGTA CCTTCAGTCA CAGGCTGTCG GAGCTGCAGC TATGGATCAC CATGGCCACA 
CAGACATTAG AGTCACACCA AGGGGATGTG CGTCTGTGGG ATGCTGAGTC CCAAGAGGCT 
GGACTCGAGA CGCTGCTGTC TGAAATCCCA GAGAAAGAGG TCCAGGTGTC CCTGCTCCAA 
GCACTGGGCC AGCTTGTGAT GAAGAAGTCT TCCCCAGAAG GGGCAACCAT GGTCCAGGAG 
GAGCTGAGGA AGCTGATGGA GTCTTGGCAG GCCCTGCGGC TGCTAGAGGA GAACATGCTG 
AGTCTCATGA GAAACCAGCA GCTGCAGAGG ACAGAGGTGG ACACGGGGAA GAAGCAGGTG 
TTCACCAACA ACATCCCAAA GGCCGGCTTT CTCATCAACC CTCAGGACCC CATTCCCAGG 
AGACAGCATG GGGCAAACCC ACTGGAAGGA CACGACCTCC CTGAAGATCA TCCCCAGCTC 
CTGAGGGACT TTGAACAGTG GCTGCAGGCA GAAAACTCCA AGCTACGTAG AATCATCACA 
ATGAGAGTGG CCACAGCCAA GGACTTGAGG ACCAGAGAGG TGAAGCTGCA GGAGCTGGAG 
GCCCGAATCC CAGAAGGCCA GCACCTCTTT GAGAACCTGC TTCGTCTCAG GCCGGCAAGG 
GACCCCTCCA ACGAGCTGGA AGATCTGCGC TACCGGTGGA TGCTGTACAA GTCCAAGCTC 
AAGGACTCTG GCCACCTGCT GACCGAGAGT TCTCCGGGGG AGCTGACTGC ATTCCAGAAG 
AGTCGGAGGC AGAAGCGGTG GAGTCCCTGC TCTCTCCTAC AGAAAGCATG CCGTGTGGCA 
CTGCCATTGC AGCTGTTGCT CCTGCTCTTT CTGCTGCTGC TGTTCCTGCT GCCGGCCGGC 
GAGGAGGAGC GCAGCTGCGC CCTGGCCAAC AACTTCGCCC GCTCCTTTGC GCTCATGCTT 
CGGTACAATG GCCCCCCGCC CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766088.2
CDS	199..2865
Translation	MTTRRGPERPWRQGFERQSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQVAQDHEQYREDVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESLKRLEEQAVGVIQNTSPLGAEKISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCEQQIQQLEAELGDFKKSLQRLAQEGLEPTVKTATEDELVAQWRLFSGTRAALASEEPRVDRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATRAELWQRFQRPLNDLQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGSIFGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDLQARIQAEKGLPRDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQISCDQQALQRSLEDLVDRCQQNVREHCTFSHRLSELQLWITMATQTLESHQGDVRLWDAESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKSSPEGATMVQEELRKLMESWQALRLLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPRRQHGANPLEGHDLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHLFENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRWSPCSLLQKACRVALPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGPPPT

Target ORF information:

RefSeq Version	NM_172500.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172500.3

ORF Insert Sequence:

ATGACAACAC GAAGGGGCCC CGAGCGGCCC TGGAGGCAAG GCTTCGAGAG ACAGAGTCGC 
ATCGAGTGGG TGTGGCTGCA CTGGAGTGAG TACCTGCTGG CCCAGGATGA GTTTTACCGC 
TGGTTCCAGA AGATGGTGGT CGCACTGGAG CCCCCCGTGG AGCTGCAGCT GGGCTTGAAG 
GAGAAGCAAT GGCAGCTGAG CCACGCCCAG GTGCTGCTGC ACAACGTGGA CAATCAGGCT 
GTGCTCCTGG ACAGGCTGTT GGAGGAGGCG GGCTCCCTGT TCAGCAGGAT CGGAGACCCC 
AGCGTGGATG AAGATGCCCA GAAGAGGATG AAGGCTGAGT ACGATGCCGT GAAGGCCAGA 
GCCCAGCGCA GGGTGGACCT CCTGGCCCAG GTGGCCCAGG ACCATGAGCA GTACCGGGAG 
GACGTGAATG AGTTCCAGCT GTGGCTGAAG GCGGTGGTGG AGAAGGTGCA CAGCTGTCTG 
GGGCGGAACT GCAAGCTGGC CACAGAACTT CGTCTCTCTA CGCTGCAGGA CATCGCCAAG 
GATTTCCCTA GGGGTGAGGA GTCTCTGAAA AGATTGGAGG AACAGGCTGT GGGTGTCATT 
CAAAACACCT CTCCCTTGGG TGCAGAGAAG ATCTCAGGGG AGCTGGAGGA GATGCGGGGT 
GTCCTGGAGA AGCTGAGAGT CCTCTGGAAA GAGGAGGAAG GGAGGCTGCG GGGCCTGCTC 
CAGTCCAGGG GGGACTGTGA GCAGCAGATC CAACAGCTGG AGGCAGAACT GGGAGACTTC 
AAGAAAAGCC TTCAGAGGCT GGCCCAGGAG GGCTTGGAGC CCACGGTGAA GACAGCCACA 
GAGGATGAGC TGGTGGCCCA GTGGAGGCTG TTCTCGGGGA CTCGGGCTGC ACTGGCTTCA 
GAGGAACCCC GTGTAGACCG GTTACAAACT CAACTGAAGA AACTTGTCAC CTTCCCGGAC 
CTGCAGTCAC TCTCTGACAG CGTGGTAGCC ACCATTCAGG AATACCAAAG TATGAAGGGG 
AAGAATACCA GGCTCCACAA TGCGACCCGG GCAGAGCTGT GGCAGCGTTT CCAGCGGCCC 
CTAAATGACC TGCAGCTGTG GAAGGCCCTG GCCCAGAGGC TCCTGGACAT CACGGCCAGC 
CTGCCTGACC TGGCCTCCAT TCACACCTTT CTACCCCAGA TTGAGGCGGC CCTCACGGAA 
AGCTCTCGCC TGAAAGAGCA GCTGGCGATG CTGCAGCTGA AGACCGACCT GCTGGGCAGC 
ATCTTTGGCC AGGAGAGAGC AGCCACCCTC CTGGAGCAGG TGACAAGTTC TGTGAGGGAC 
AGAGACCTAC TGCATAACAG CCTTCTTCAG CGGAAGAGCA AACTTCAGAG CCTGCTTGTT 
CAGCACAAGG ACTTTGGGGT GGCTTTTGAT CCCCTAAACA GGAAGCTCCT AGACCTCCAG 
GCCAGGATCC AAGCAGAGAA AGGGCTTCCG AGGGACCTTC CTGGAAAGCA GGTCCAGCTC 
CTAAGGTTGC AGGGGCTGCA GGAAGAGGGG CTGGATCTGG GGACACAGAT CGAGGCTGTG 
AGGCCTCTTG CCCATGGGAA CTCTAAGCAC CAGCAGAAAG TAGACCAGAT CTCCTGTGAC 
CAGCAAGCCC TGCAGAGGTC CCTGGAGGAT CTCGTGGACA GGTGTCAGCA GAACGTACGG 
GAACATTGTA CCTTCAGTCA CAGGCTGTCG GAGCTGCAGC TATGGATCAC CATGGCCACA 
CAGACATTAG AGTCACACCA AGGGGATGTG CGTCTGTGGG ATGCTGAGTC CCAAGAGGCT 
GGACTCGAGA CGCTGCTGTC TGAAATCCCA GAGAAAGAGG TCCAGGTGTC CCTGCTCCAA 
GCACTGGGCC AGCTTGTGAT GAAGAAGTCT TCCCCAGAAG GGGCAACCAT GGTCCAGGAG 
GAGCTGAGGA AGCTGATGGA GTCTTGGCAG GCCCTGCGGC TGCTAGAGGA GAACATGCTG 
AGTCTCATGA GAAACCAGCA GCTGCAGAGG ACAGAGGTGG ACACGGGGAA GAAGCAGGTG 
TTCACCAACA ACATCCCAAA GGCCGGCTTT CTCATCAACC CTCAGGACCC CATTCCCAGG 
AGACAGCATG GGGCAAACCC ACTGGAAGGA CACGACCTCC CTGAAGATCA TCCCCAGCTC 
CTGAGGGACT TTGAACAGTG GCTGCAGGCA GAAAACTCCA AGCTACGTAG AATCATCACA 
ATGAGAGTGG CCACAGCCAA GGACTTGAGG ACCAGAGAGG TGAAGCTGCA GGAGCTGGAG 
GCCCGAATCC CAGAAGGCCA GCACCTCTTT GAGAACCTGC TTCGTCTCAG GCCGGCAAGG 
GACCCCTCCA ACGAGCTGGA AGATCTGCGC TACCGGTGGA TGCTGTACAA GTCCAAGCTC 
AAGGACTCTG GCCACCTGCT GACCGAGAGT TCTCCGGGGG AGCTGACTGC ATTCCAGAAG 
AGTCGGAGGC AGAAGCGGTG GAGTCCCTGC TCTCTCCTAC AGAAAGCATG CCGTGTGGCA 
CTGCCATTGC AGCTGTTGCT CCTGCTCTTT CTGCTGCTGC TGTTCCTGCT GCCGGCCGGC 
GAGGAGGAGC GCAGCTGCGC CCTGGCCAAC AACTTCGCCC GCTCCTTTGC GCTCATGCTT 
CGGTACAATG GCCCCCCGCC CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syne3 ( NM_001042699.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001036164.1 Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14134
Clone ID Related Accession (Same CDS sequence)	XM_006515653.2 , XM_006515654.4 , NM_001042699.2 , XM_006515652.3 , XM_006515654.3 , XM_006515653.3
Accession Version	NM_001042699.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	nesprin-3 isoform alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC099694.1, AK030542.1, BB733613.1 and AC147375.3. On Nov 6, 2013 this sequence version replaced NM_001042699.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC099694.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036164.1
CDS	293..3220
Translation	MTQQPQEDFERSVEDAQAWMKVIQEQLQVNDNTKGPRAALEARLRETEKICQLESEGMVKVELVLRAAEALLATCQEGQKPEILARLRDIKSQWEETVTYMTHCHSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQVAQDHEQYREDVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESLKRLEEQAVGVIQNTSPLGAEKISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCEQQIQQLEAELGDFKKSLQRLAQEGLEPTVKTATEDELVAQWRLFSGTRAALASEEPRVDRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATRAELWQRFQRPLNDLQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGSIFGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDLQARIQAEKGLPRDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQISCDQQALQRSLEDLVDRCQQNVREHCTFSHRLSELQLWITMATQTLESHQGDVRLWDAESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKSSPEGATMVQEELRKLMESWQALRLLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPRRQHGANPLEGHDLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHLFENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRWSPCSLLQKACRVALPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGPPPT

Target ORF information:

RefSeq Version	NM_001042699.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042699.2

ORF Insert Sequence:

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syne3 ( XM_006515653.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515716.1 Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu14134
Clone ID Related Accession (Same CDS sequence)	XM_006515653.2 , XM_006515654.4 , NM_001042699.2 , XM_006515652.3 , XM_006515654.3 , XM_006515653.3
Accession Version	XM_006515653.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nesprin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515716.1
CDS	222..3149
Translation	MTQQPQEDFERSVEDAQAWMKVIQEQLQVNDNTKGPRAALEARLRETEKICQLESEGMVKVELVLRAAEALLATCQEGQKPEILARLRDIKSQWEETVTYMTHCHSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQVAQDHEQYREDVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESLKRLEEQAVGVIQNTSPLGAEKISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCEQQIQQLEAELGDFKKSLQRLAQEGLEPTVKTATEDELVAQWRLFSGTRAALASEEPRVDRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATRAELWQRFQRPLNDLQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGSIFGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDLQARIQAEKGLPRDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQISCDQQALQRSLEDLVDRCQQNVREHCTFSHRLSELQLWITMATQTLESHQGDVRLWDAESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKSSPEGATMVQEELRKLMESWQALRLLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPRRQHGANPLEGHDLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHLFENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRWSPCSLLQKACRVALPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGPPPT

Target ORF information:

RefSeq Version	XM_006515653.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515653.2

ORF Insert Sequence:

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syne3 ( XM_006515652.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515715.2 Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu14134
Clone ID Related Accession (Same CDS sequence)	XM_006515653.2 , XM_006515654.4 , NM_001042699.2 , XM_006515652.3 , XM_006515654.3 , XM_006515653.3
Accession Version	XM_006515652.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nesprin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515715.2
CDS	123..3050
Translation	MTQQPQEDFERSVEDAQAWMKVIQEQLQVNDNTKGPRAALEARLRETEKICQLESEGMVKVELVLRAAEALLATCQEGQKPEILARLRDIKSQWEETVTYMTHCHSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQVAQDHEQYREDVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESLKRLEEQAVGVIQNTSPLGAEKISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCEQQIQQLEAELGDFKKSLQRLAQEGLEPTVKTATEDELVAQWRLFSGTRAALASEEPRVDRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATRAELWQRFQRPLNDLQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGSIFGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDLQARIQAEKGLPRDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQISCDQQALQRSLEDLVDRCQQNVREHCTFSHRLSELQLWITMATQTLESHQGDVRLWDAESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKSSPEGATMVQEELRKLMESWQALRLLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPRRQHGANPLEGHDLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHLFENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRWSPCSLLQKACRVALPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGPPPT

Target ORF information:

RefSeq Version	XM_006515652.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515652.3

ORF Insert Sequence:

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syne3 ( XM_006515654.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515717.1 Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu14134
Clone ID Related Accession (Same CDS sequence)	XM_006515653.2 , XM_006515654.4 , NM_001042699.2 , XM_006515652.3 , XM_006515654.3 , XM_006515653.3
Accession Version	XM_006515654.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nesprin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515654.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515717.1
CDS	343..3270
Translation	MTQQPQEDFERSVEDAQAWMKVIQEQLQVNDNTKGPRAALEARLRETEKICQLESEGMVKVELVLRAAEALLATCQEGQKPEILARLRDIKSQWEETVTYMTHCHSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQVAQDHEQYREDVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESLKRLEEQAVGVIQNTSPLGAEKISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCEQQIQQLEAELGDFKKSLQRLAQEGLEPTVKTATEDELVAQWRLFSGTRAALASEEPRVDRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATRAELWQRFQRPLNDLQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGSIFGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDLQARIQAEKGLPRDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQISCDQQALQRSLEDLVDRCQQNVREHCTFSHRLSELQLWITMATQTLESHQGDVRLWDAESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKSSPEGATMVQEELRKLMESWQALRLLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPRRQHGANPLEGHDLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHLFENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRWSPCSLLQKACRVALPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGPPPT

Target ORF information:

RefSeq Version	XM_006515654.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515654.3

ORF Insert Sequence:

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syne3 ( XM_006515654.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515717.1 Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu14134
Clone ID Related Accession (Same CDS sequence)	XM_006515653.2 , XM_006515654.4 , NM_001042699.2 , XM_006515652.3 , XM_006515654.3 , XM_006515653.3
Accession Version	XM_006515654.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nesprin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515654.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515717.1
CDS	325..3252
Translation	MTQQPQEDFERSVEDAQAWMKVIQEQLQVNDNTKGPRAALEARLRETEKICQLESEGMVKVELVLRAAEALLATCQEGQKPEILARLRDIKSQWEETVTYMTHCHSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQVAQDHEQYREDVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESLKRLEEQAVGVIQNTSPLGAEKISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCEQQIQQLEAELGDFKKSLQRLAQEGLEPTVKTATEDELVAQWRLFSGTRAALASEEPRVDRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATRAELWQRFQRPLNDLQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGSIFGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDLQARIQAEKGLPRDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQISCDQQALQRSLEDLVDRCQQNVREHCTFSHRLSELQLWITMATQTLESHQGDVRLWDAESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKSSPEGATMVQEELRKLMESWQALRLLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPRRQHGANPLEGHDLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHLFENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRWSPCSLLQKACRVALPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGPPPT

Target ORF information:

RefSeq Version	XM_006515654.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515654.4

ORF Insert Sequence:

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syne3 ( XM_006515653.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515716.1 Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X2, mRNA.

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CloneID	OMu14134
Clone ID Related Accession (Same CDS sequence)	XM_006515653.2 , XM_006515654.4 , NM_001042699.2 , XM_006515652.3 , XM_006515654.3 , XM_006515653.3
Accession Version	XM_006515653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nesprin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515716.1
CDS	222..3149
Translation	MTQQPQEDFERSVEDAQAWMKVIQEQLQVNDNTKGPRAALEARLRETEKICQLESEGMVKVELVLRAAEALLATCQEGQKPEILARLRDIKSQWEETVTYMTHCHSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQVAQDHEQYREDVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESLKRLEEQAVGVIQNTSPLGAEKISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCEQQIQQLEAELGDFKKSLQRLAQEGLEPTVKTATEDELVAQWRLFSGTRAALASEEPRVDRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATRAELWQRFQRPLNDLQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGSIFGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDLQARIQAEKGLPRDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQISCDQQALQRSLEDLVDRCQQNVREHCTFSHRLSELQLWITMATQTLESHQGDVRLWDAESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKSSPEGATMVQEELRKLMESWQALRLLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPRRQHGANPLEGHDLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHLFENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRWSPCSLLQKACRVALPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGPPPT

Target ORF information:

RefSeq Version	XM_006515653.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515653.3

ORF Insert Sequence:

ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Syne3 ( XM_006515652.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515715.3 Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X1, mRNA.

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CloneID	OMu86023
Clone ID Related Accession (Same CDS sequence)	XM_006515652.4
Accession Version	XM_006515652.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nesprin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515652.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGC TGGATCGGAT CTGGATTGGC ATGGACAGAC ACAGTATTCC CGCCCTCCTG 
AACTTTGGTT TGAGAATTGT AGCGGGGAGG TTCGTCCTCC GCCCTGGTTC CCCATTTGCA 
GTGTGGCGGG GCTCCGGGCG GGTCCCTAGG GGGGCGGGGC AGTCGCGGTG TTCCCCGCCC 
CGCCCTGACC CGGCCCCGCC CCGCCCCGGA GCCGCCCGGG ACGCAGGGTG CAGGAGGGAC 
TCGGCCCAGC CGCCGGAGGC GCCGCACTCC GTCTGCTCCG GGCAGGCCCT CGCAGGTGCG 
ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515715.3
CDS	312..3539
Translation	MKTLDRIWIGMDRHSIPALLNFGLRIVAGRFVLRPGSPFAVWRGSGRVPRGAGQSRCSPPRPDPAPPRPGAARDAGCRRDSAQPPEAPHSVCSGQALAGAMTQQPQEDFERSVEDAQAWMKVIQEQLQVNDNTKGPRAALEARLRETEKICQLESEGMVKVELVLRAAEALLATCQEGQKPEILARLRDIKSQWEETVTYMTHCHSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQVAQDHEQYREDVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESLKRLEEQAVGVIQNTSPLGAEKISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCEQQIQQLEAELGDFKKSLQRLAQEGLEPTVKTATEDELVAQWRLFSGTRAALASEEPRVDRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATRAELWQRFQRPLNDLQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGSIFGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDLQARIQAEKGLPRDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQISCDQQALQRSLEDLVDRCQQNVREHCTFSHRLSELQLWITMATQTLESHQGDVRLWDAESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKSSPEGATMVQEELRKLMESWQALRLLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPRRQHGANPLEGHDLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHLFENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRWSPCSLLQKACRVALPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGPPPT

Target ORF information:

RefSeq Version	XM_006515652.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin repeat containing, nuclear envelope family member 3 (Syne3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515652.4

ORF Insert Sequence:

ATGAAGACGC TGGATCGGAT CTGGATTGGC ATGGACAGAC ACAGTATTCC CGCCCTCCTG 
AACTTTGGTT TGAGAATTGT AGCGGGGAGG TTCGTCCTCC GCCCTGGTTC CCCATTTGCA 
GTGTGGCGGG GCTCCGGGCG GGTCCCTAGG GGGGCGGGGC AGTCGCGGTG TTCCCCGCCC 
CGCCCTGACC CGGCCCCGCC CCGCCCCGGA GCCGCCCGGG ACGCAGGGTG CAGGAGGGAC 
TCGGCCCAGC CGCCGGAGGC GCCGCACTCC GTCTGCTCCG GGCAGGCCCT CGCAGGTGCG 
ATGACGCAGC AGCCCCAGGA GGACTTTGAG AGGAGCGTGG AGGATGCCCA GGCCTGGATG 
AAGGTGATAC AGGAGCAGCT TCAGGTCAAT GACAACACGA AGGGGCCCCG AGCGGCCCTG 
GAGGCAAGGC TTCGAGAGAC AGAGAAAATC TGCCAGCTGG AGTCTGAAGG AATGGTGAAG 
GTGGAACTGG TCCTGCGGGC GGCGGAGGCC CTCTTGGCAA CTTGCCAGGA GGGCCAGAAA 
CCTGAGATCC TGGCCCGGCT GAGGGATATC AAGTCTCAGT GGGAGGAGAC GGTCACCTAC 
ATGACCCACT GCCACAGTCG CATCGAGTGG GTGTGGCTGC ACTGGAGTGA GTACCTGCTG 
GCCCAGGATG AGTTTTACCG CTGGTTCCAG AAGATGGTGG TCGCACTGGA GCCCCCCGTG 
GAGCTGCAGC TGGGCTTGAA GGAGAAGCAA TGGCAGCTGA GCCACGCCCA GGTGCTGCTG 
CACAACGTGG ACAATCAGGC TGTGCTCCTG GACAGGCTGT TGGAGGAGGC GGGCTCCCTG 
TTCAGCAGGA TCGGAGACCC CAGCGTGGAT GAAGATGCCC AGAAGAGGAT GAAGGCTGAG 
TACGATGCCG TGAAGGCCAG AGCCCAGCGC AGGGTGGACC TCCTGGCCCA GGTGGCCCAG 
GACCATGAGC AGTACCGGGA GGACGTGAAT GAGTTCCAGC TGTGGCTGAA GGCGGTGGTG 
GAGAAGGTGC ACAGCTGTCT GGGGCGGAAC TGCAAGCTGG CCACAGAACT TCGTCTCTCT 
ACGCTGCAGG ACATCGCCAA GGATTTCCCT AGGGGTGAGG AGTCTCTGAA AAGATTGGAG 
GAACAGGCTG TGGGTGTCAT TCAAAACACC TCTCCCTTGG GTGCAGAGAA GATCTCAGGG 
GAGCTGGAGG AGATGCGGGG TGTCCTGGAG AAGCTGAGAG TCCTCTGGAA AGAGGAGGAA 
GGGAGGCTGC GGGGCCTGCT CCAGTCCAGG GGGGACTGTG AGCAGCAGAT CCAACAGCTG 
GAGGCAGAAC TGGGAGACTT CAAGAAAAGC CTTCAGAGGC TGGCCCAGGA GGGCTTGGAG 
CCCACGGTGA AGACAGCCAC AGAGGATGAG CTGGTGGCCC AGTGGAGGCT GTTCTCGGGG 
ACTCGGGCTG CACTGGCTTC AGAGGAACCC CGTGTAGACC GGTTACAAAC TCAACTGAAG 
AAACTTGTCA CCTTCCCGGA CCTGCAGTCA CTCTCTGACA GCGTGGTAGC CACCATTCAG 
GAATACCAAA GTATGAAGGG GAAGAATACC AGGCTCCACA ATGCGACCCG GGCAGAGCTG 
TGGCAGCGTT TCCAGCGGCC CCTAAATGAC CTGCAGCTGT GGAAGGCCCT GGCCCAGAGG 
CTCCTGGACA TCACGGCCAG CCTGCCTGAC CTGGCCTCCA TTCACACCTT TCTACCCCAG 
ATTGAGGCGG CCCTCACGGA AAGCTCTCGC CTGAAAGAGC AGCTGGCGAT GCTGCAGCTG 
AAGACCGACC TGCTGGGCAG CATCTTTGGC CAGGAGAGAG CAGCCACCCT CCTGGAGCAG 
GTGACAAGTT CTGTGAGGGA CAGAGACCTA CTGCATAACA GCCTTCTTCA GCGGAAGAGC 
AAACTTCAGA GCCTGCTTGT TCAGCACAAG GACTTTGGGG TGGCTTTTGA TCCCCTAAAC 
AGGAAGCTCC TAGACCTCCA GGCCAGGATC CAAGCAGAGA AAGGGCTTCC GAGGGACCTT 
CCTGGAAAGC AGGTCCAGCT CCTAAGGTTG CAGGGGCTGC AGGAAGAGGG GCTGGATCTG 
GGGACACAGA TCGAGGCTGT GAGGCCTCTT GCCCATGGGA ACTCTAAGCA CCAGCAGAAA 
GTAGACCAGA TCTCCTGTGA CCAGCAAGCC CTGCAGAGGT CCCTGGAGGA TCTCGTGGAC 
AGGTGTCAGC AGAACGTACG GGAACATTGT ACCTTCAGTC ACAGGCTGTC GGAGCTGCAG 
CTATGGATCA CCATGGCCAC ACAGACATTA GAGTCACACC AAGGGGATGT GCGTCTGTGG 
GATGCTGAGT CCCAAGAGGC TGGACTCGAG ACGCTGCTGT CTGAAATCCC AGAGAAAGAG 
GTCCAGGTGT CCCTGCTCCA AGCACTGGGC CAGCTTGTGA TGAAGAAGTC TTCCCCAGAA 
GGGGCAACCA TGGTCCAGGA GGAGCTGAGG AAGCTGATGG AGTCTTGGCA GGCCCTGCGG 
CTGCTAGAGG AGAACATGCT GAGTCTCATG AGAAACCAGC AGCTGCAGAG GACAGAGGTG 
GACACGGGGA AGAAGCAGGT GTTCACCAAC AACATCCCAA AGGCCGGCTT TCTCATCAAC 
CCTCAGGACC CCATTCCCAG GAGACAGCAT GGGGCAAACC CACTGGAAGG ACACGACCTC 
CCTGAAGATC ATCCCCAGCT CCTGAGGGAC TTTGAACAGT GGCTGCAGGC AGAAAACTCC 
AAGCTACGTA GAATCATCAC AATGAGAGTG GCCACAGCCA AGGACTTGAG GACCAGAGAG 
GTGAAGCTGC AGGAGCTGGA GGCCCGAATC CCAGAAGGCC AGCACCTCTT TGAGAACCTG 
CTTCGTCTCA GGCCGGCAAG GGACCCCTCC AACGAGCTGG AAGATCTGCG CTACCGGTGG 
ATGCTGTACA AGTCCAAGCT CAAGGACTCT GGCCACCTGC TGACCGAGAG TTCTCCGGGG 
GAGCTGACTG CATTCCAGAA GAGTCGGAGG CAGAAGCGGT GGAGTCCCTG CTCTCTCCTA 
CAGAAAGCAT GCCGTGTGGC ACTGCCATTG CAGCTGTTGC TCCTGCTCTT TCTGCTGCTG 
CTGTTCCTGC TGCCGGCCGG CGAGGAGGAG CGCAGCTGCG CCCTGGCCAA CAACTTCGCC 
CGCTCCTTTG CGCTCATGCT TCGGTACAAT GGCCCCCCGC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

p63 Transcription Factor Regulates Nuclear?Shape and Expression of Nuclear?Envelope-Associated Genes in Epidermal Keratinocytes.
The Journal of investigative dermatology137(10)2157-2167(2017 Oct)
Rapisarda V,Malashchuk I,Asamaowei IE,Poterlowicz K,Fessing MY,Sharov AA,Karakesisoglou I,Botchkarev VA,Mardaryev A

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Nesprin-3 connects plectin and vimentin to the nuclear envelope of Sertoli cells but is not required for Sertoli cell function in spermatogenesis.
Molecular biology of the cell24(15)2454-66(2013 Aug)
Ketema M,Kreft M,Secades P,Janssen H,Sonnenberg A

The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction.
Scientific reports31087(2013)
Chambliss AB,Khatau SB,Erdenberger N,Robinson DK,Hodzic D,Longmore GD,Wirtz D

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Syne3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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