Thsd7b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Thsd7b
Entrez Gene ID	210417
Full Name	thrombospondin, type I, domain containing 7B
Synonyms	1700074E13Rik,B930082A18,D130067I03Rik
General protein information	Preferred Names thrombospondin, type I, domain containing 7B Names thrombospondin type-1 domain-containing protein 7B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_172485.3	NP_766073.2	thrombospondin type-1 domain-containing protein 7B precursor
XM_011247964.1	XP_011246266.1	thrombospondin type-1 domain-containing protein 7B isoform X1
XM_011247965.1	XP_011246267.1	thrombospondin type-1 domain-containing protein 7B isoform X1
XM_011247966.2	XP_011246268.1	thrombospondin type-1 domain-containing protein 7B isoform X2
XM_011247966.3	XP_011246268.1	thrombospondin type-1 domain-containing protein 7B isoform X2
XM_011247964.2	XP_011246266.1	thrombospondin type-1 domain-containing protein 7B isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THSD7B	XP_515814.4
Gallus gallus (chicken)	THSD7B	XP_422142.4
Danio rerio (zebrafish)	LOC566298	XP_694660.6
Xenopus tropicalis (tropical clawed frog)	thsd7b	XP_002933997.2
Homo sapiens (human)	THSD7B	NP_001073896.1
Canis lupus familiaris (dog)	THSD7B	XP_005632014.1
Bos taurus (cattle)	THSD7B	XP_003585799.2
Mus musculus (house mouse)	Thsd7b	NP_766073.2
Rattus norvegicus (Norway rat)	Thsd7b	NP_001178598.1

Gene Ontology
Bibliography

Related articles in PubMed

O-fucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) and maintains epiblast pluripotency during mouse gastrulation.
Du J, Takeuchi H, Leonhard-Melief C, Shroyer KR, Dlugosz M, Haltiwanger RS, Holdener BC
Developmental biology346(1)25-38(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Thsd7b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thsd7b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu72736	XM_011247966.3 Latest version!	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu03543	NM_172485.3 Latest version!	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$944.30 $1349.00
OMu72735	XM_011247964.2 Latest version!	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu72735	XM_011247965.1 Latest version!	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu72736	XM_011247966.2	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu72735	XM_011247964.1	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Thsd7b ( XM_011247966.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246268.1 Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X3, mRNA.

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CloneID	OMu72736
Clone ID Related Accession (Same CDS sequence)	XM_011247966.2 , XM_011247966.3
Accession Version	XM_011247966.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 7B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG AGAATGCTGA CAGGGAAGAC 
ACAGCAACGG GAATTTCTCC ATCTTTATCT CTGGCCAGGA GGCAGACACA GAGGAGTATC 
GATATTCATC TGCGTTTCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246268.1
CDS	173..2776
Translation	MFLRSDLAVTHWVSRSMRKLFLVLSLLLSQAAHLEGRKDNQFLWKTGPWGRCAGDCGPGGAQSRAVWCFHIEGWTSPMSNCDESSQPPKERSCFRVCDWHSDLFQWEVSDWHRCLLVPGAQGEPRPRAVECVTAQHGLQHRTVRCLQKLNRTMVSNEICEHFAPQPPTEQACLIPCPRDCVVSEFSPWSTCPEGCGKKLQHRTRVAIAPPLYGGLQCPNLTESRACEAPVSCPLGKEEYSFSLKVGPWSKCRLPHLKEVDLSGRNIQDFSSDSNEQVTLTHQSYKAHHHSQPGDVVIGFQTRQVWCTRSDGRNALLSLCVRDSFPLTVQPCVMPKDCETSEWSPWSPCSKTCRSGTLSPGVRSRSRNVKHIAIGGGQECPELLEKETCIAEGEFLQPCPRYSWRTSEWKECQVSLLLEQHDPLWHETGPICGGGIQTREVYCAQSLPATIASRTKEVSRPVERTLCLGPAPSASQLCNVPCSMDCIVSSWSTWGPCVFENCHDPQGKKGFRMRQRHVLMESTGPMGRCPHLAESVPCEDPMCHRWLASEGICIADHGKCGLGHRILKAVCQNERGEEVSGGLCPVPPPPERMACEIPCRMDCVVSEWTVWSSCSQSCSNKNSDGKQTRSRSILALAGEGGKTCPSSQELQEYRLCNDHSCTQLYWETSAWGSCSENTLVTALNVTIGWNGEATCGVGIQTRKVFCIKSHVGQVMTKRCPESTRPETVRPCFLPCKKDCLVTAFSEWTPCPRPCQPGNTTIKQSRYRIIIQEAANGGQECPDTLFEERECEDISLCPSYRWKPQKWSSCILVPESIRQGRTGTSEACGKGLQTRENADREDTATGISPSLSLARRQTQRSIDIHLRFS

Target ORF information:

RefSeq Version	XM_011247966.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247966.2

ORF Insert Sequence:

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG AGAATGCTGA CAGGGAAGAC 
ACAGCAACGG GAATTTCTCC ATCTTTATCT CTGGCCAGGA GGCAGACACA GAGGAGTATC 
GATATTCATC TGCGTTTCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd7b ( XM_011247966.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246268.1 Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu72736
Clone ID Related Accession (Same CDS sequence)	XM_011247966.2 , XM_011247966.3
Accession Version	XM_011247966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 7B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG AGAATGCTGA CAGGGAAGAC 
ACAGCAACGG GAATTTCTCC ATCTTTATCT CTGGCCAGGA GGCAGACACA GAGGAGTATC 
GATATTCATC TGCGTTTCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246268.1
CDS	446..3049
Translation	MFLRSDLAVTHWVSRSMRKLFLVLSLLLSQAAHLEGRKDNQFLWKTGPWGRCAGDCGPGGAQSRAVWCFHIEGWTSPMSNCDESSQPPKERSCFRVCDWHSDLFQWEVSDWHRCLLVPGAQGEPRPRAVECVTAQHGLQHRTVRCLQKLNRTMVSNEICEHFAPQPPTEQACLIPCPRDCVVSEFSPWSTCPEGCGKKLQHRTRVAIAPPLYGGLQCPNLTESRACEAPVSCPLGKEEYSFSLKVGPWSKCRLPHLKEVDLSGRNIQDFSSDSNEQVTLTHQSYKAHHHSQPGDVVIGFQTRQVWCTRSDGRNALLSLCVRDSFPLTVQPCVMPKDCETSEWSPWSPCSKTCRSGTLSPGVRSRSRNVKHIAIGGGQECPELLEKETCIAEGEFLQPCPRYSWRTSEWKECQVSLLLEQHDPLWHETGPICGGGIQTREVYCAQSLPATIASRTKEVSRPVERTLCLGPAPSASQLCNVPCSMDCIVSSWSTWGPCVFENCHDPQGKKGFRMRQRHVLMESTGPMGRCPHLAESVPCEDPMCHRWLASEGICIADHGKCGLGHRILKAVCQNERGEEVSGGLCPVPPPPERMACEIPCRMDCVVSEWTVWSSCSQSCSNKNSDGKQTRSRSILALAGEGGKTCPSSQELQEYRLCNDHSCTQLYWETSAWGSCSENTLVTALNVTIGWNGEATCGVGIQTRKVFCIKSHVGQVMTKRCPESTRPETVRPCFLPCKKDCLVTAFSEWTPCPRPCQPGNTTIKQSRYRIIIQEAANGGQECPDTLFEERECEDISLCPSYRWKPQKWSSCILVPESIRQGRTGTSEACGKGLQTRENADREDTATGISPSLSLARRQTQRSIDIHLRFS

Target ORF information:

RefSeq Version	XM_011247966.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247966.3

ORF Insert Sequence:

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG AGAATGCTGA CAGGGAAGAC 
ACAGCAACGG GAATTTCTCC ATCTTTATCT CTGGCCAGGA GGCAGACACA GAGGAGTATC 
GATATTCATC TGCGTTTCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd7b ( NM_172485.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766073.2 Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), mRNA.

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CloneID	OMu03543
Clone ID Related Accession (Same CDS sequence)	NM_172485.3
Accession Version	NM_172485.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 7B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB658510.1, BC063250.1 and AC110536.10. On May 15, 2009 this sequence version replaced NM_172485.2. ##Evidence-Data-START## Transcript exon combination :: BC063250.1, SRR1660813.270776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG CTGTCTCATG CATCTCTGAT 
GATAACCAGT CTGCAGAAAT GACAGAGTGC CTCAAGCAGA TGAATGGCAT GCCTCCCCTT 
GTGCAGGAAT GCACCATTCC CTGTCGGGAC GACTGCACCT TCACCCCTTG GTCTAAGTTC 
ACTCCCTGCT CCAAGAACTG TGAAGCAACT CAGATCAGGA GGCGGCAGCT AACAGGGAAA 
AGCAGGAAGA AAGAGAAGTG CCAGGATGCG TCCCTGTACC CTCTGGTGGA AATGGAACCA 
TGTCCCTGTG ATACGTTTAT GTCTCATCCT TATGGAAACT GGTCAGCTTG CATTCTACCA 
GAAGGCAAAA GGGATGCTCA GCAAGGAGGA TTGTGGGTGC AAGGAGATGA CAAAGAATGT 
GGAGAAGGCG TGCGCTTTCG AGCAATAGCA TGCTCCAATA TAAATGGAAG ACCGGTTGAC 
CCCTCATTCT GCAACAGCTC TGGTTACATT CAGGAAGCAT GTGTCATCCC CTGTCCGTTT 
GATTGCAAGC TAAGTGACTG GTCCAGCTGG GGTTCTTGCA GCTCATCTTG TGGGATTGGA 
GTGAGAATTC GATCAAAATG GCTAAAAGAA AAACCTTACA GTGGAGGTCG GCCATGTCCC 
AAACTGGATC TCAAGAATCA GGTTCATGAG GCAGTCCCAT GTTACAGTGA GTGTGATCAA 
TATTCCTGGG TCGTAGAACA CTGGTCTCCA TGCAAGATCA ATAATGAGCT GAGATCCCCA 
CGCTGTGGAA GAGGAACACA GTCTAGAAGA ATCAGGTGTG TGAGTACTGC TGACAGAGAA 
GGAGGGGCAG TGAACAGGAG CCTGTGCAAC CAGGATGATG CTCCCCAAGA AACCCAGGCC 
TGTTCTCTTC TGTGTCCCAG TGAGTGTGTC ATGTCCGAGT GGGGAACATG GAGCAGATGC 
CCACAGTCAT GTGACCCCCA TGCAATGCAG AGAAGAACTC GGCATCTGCT GAGGCCCTCA 
CTGAACTCAA GAACTTGTGG GGAAGACTCG CAGGTGCGGC CCTGCCTCCT CAATGAAAAC 
TGTTTCCAGT TCCAGTACAA TCTGACAGAG TGGAGCACAT GCCAGCTGAG TGAAAATGTT 
TCCTGTGGCC AGGGTGTGAG GACCCGCCTG CTGAGCTGTG TGCGCAGTGA TGGCAAGTCT 
GTCAGCATGG ACCACTGCGA GCAGCGTAAT CTGGAAAAAC CCCAGAGGAT GAGTATTCCC 
TGTCTGGTGG AATGTGTGGT CAACTGTCAG CTCTCAGGAT GGACAACATG GACAGAGTGT 
TCACAAACCT GTGGCCAAGG AGGTCGCATG AGCCGGACTC GATTTATCAT TATGCCAACC 
CAAGGAGAAG GTCGACAATG CCCCACAGAA CTCACCCAGC AGAAACCCTG CCCAGTGACA 
CCCTGCTATA GTTGGGTCCT TGGCAACTGG TCTGCATGTA AATTGGAGGG CGGAGATTGT 
GGAGAAGGAG TCCAGGTGCG CAGCTTCTCC TGTGTAGTCC ACAATGGCTC AATATCCCAC 
ACGGCTGTCC CTGTGGAGGA GGCCCTGTGT GGAGAAGTAC CCTTCCAAGA AGGAATCCTG 
AAGCAGCTGT GCTCTGTGCC TTGCCCAGGA GACTGCCACA TTACCCCATG GTCTGAGTGG 
AGCAAGTGTG AGCTAACCTG CATTGACGGA AGAAGCTTCG AGACCACAGG CCGCCAATCT 
AGGTCAAGGA CATTCATAAT CCAGTCTTTT GAGAATCAAG ATAGCTGCCC CCAACAGGTT 
CTAGAAACAC GACCATGTAC AGGTGGCAAA TGTTATCACT ACATATGGAA AGCAAGTCTT 
TGGAACAATA ATGAGCGGAC TGTGTGGTGC CAGCGCTCAG ATGGCCTTAA TGTCACAGGT 
GGCTGCTCCC CTCAGGCACG CCCTGCTGCT ATTCGGCAAT GCATTCCAGC ATGCAAAAAA 
CCTTTCTCCT ACTGCACACA GGGAGGAGTC TGTGGCTGTG AGAAGGGATA CACAGAGATA 
ATGAGATCAA GTGGCTTCCT GGATTACTGC ATGAAGGTGC CTGGCTCGGA GGATAAAAAA 
GCTGACGTGA AAAACCTCTC TGGGAAAAAT AGACCTGTGA ATTCCAAAAT ACATGATATT 
TTTAAAGGAT GGTCTCTCCA ACCTCTGGAC CCAGATGGCC GAGTAAAAAT GTGGGTTTAT 
GGCGTTTCCG GTGGCAGTTT TCTTATCATG ATCTTCCTAG TATTTACTTC CTATCTTGTT 
TGCAAGAAGC CAAAACCACA TCAAAGCACA CCTCGCCATC AGAAGCCACT GACCTTGGCC 
TACGATGGAG ACTTGGACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766073.2
CDS	332..5155
Translation	MFLRSDLAVTHWVSRSMRKLFLVLSLLLSQAAHLEGRKDNQFLWKTGPWGRCAGDCGPGGAQSRAVWCFHIEGWTSPMSNCDESSQPPKERSCFRVCDWHSDLFQWEVSDWHRCLLVPGAQGEPRPRAVECVTAQHGLQHRTVRCLQKLNRTMVSNEICEHFAPQPPTEQACLIPCPRDCVVSEFSPWSTCPEGCGKKLQHRTRVAIAPPLYGGLQCPNLTESRACEAPVSCPLGKEEYSFSLKVGPWSKCRLPHLKEVDLSGRNIQDFSSDSNEQVTLTHQSYKAHHHSQPGDVVIGFQTRQVWCTRSDGRNALLSLCVRDSFPLTVQPCVMPKDCETSEWSPWSPCSKTCRSGTLSPGVRSRSRNVKHIAIGGGQECPELLEKETCIAEGEFLQPCPRYSWRTSEWKECQVSLLLEQHDPLWHETGPICGGGIQTREVYCAQSLPATIASRTKEVSRPVERTLCLGPAPSASQLCNVPCSMDCIVSSWSTWGPCVFENCHDPQGKKGFRMRQRHVLMESTGPMGRCPHLAESVPCEDPMCHRWLASEGICIADHGKCGLGHRILKAVCQNERGEEVSGGLCPVPPPPERMACEIPCRMDCVVSEWTVWSSCSQSCSNKNSDGKQTRSRSILALAGEGGKTCPSSQELQEYRLCNDHSCTQLYWETSAWGSCSENTLVTALNVTIGWNGEATCGVGIQTRKVFCIKSHVGQVMTKRCPESTRPETVRPCFLPCKKDCLVTAFSEWTPCPRPCQPGNTTIKQSRYRIIIQEAANGGQECPDTLFEERECEDISLCPSYRWKPQKWSSCILVPESIRQGRTGTSEACGKGLQTRAVSCISDDNQSAEMTECLKQMNGMPPLVQECTIPCRDDCTFTPWSKFTPCSKNCEATQIRRRQLTGKSRKKEKCQDASLYPLVEMEPCPCDTFMSHPYGNWSACILPEGKRDAQQGGLWVQGDDKECGEGVRFRAIACSNINGRPVDPSFCNSSGYIQEACVIPCPFDCKLSDWSSWGSCSSSCGIGVRIRSKWLKEKPYSGGRPCPKLDLKNQVHEAVPCYSECDQYSWVVEHWSPCKINNELRSPRCGRGTQSRRIRCVSTADREGGAVNRSLCNQDDAPQETQACSLLCPSECVMSEWGTWSRCPQSCDPHAMQRRTRHLLRPSLNSRTCGEDSQVRPCLLNENCFQFQYNLTEWSTCQLSENVSCGQGVRTRLLSCVRSDGKSVSMDHCEQRNLEKPQRMSIPCLVECVVNCQLSGWTTWTECSQTCGQGGRMSRTRFIIMPTQGEGRQCPTELTQQKPCPVTPCYSWVLGNWSACKLEGGDCGEGVQVRSFSCVVHNGSISHTAVPVEEALCGEVPFQEGILKQLCSVPCPGDCHITPWSEWSKCELTCIDGRSFETTGRQSRSRTFIIQSFENQDSCPQQVLETRPCTGGKCYHYIWKASLWNNNERTVWCQRSDGLNVTGGCSPQARPAAIRQCIPACKKPFSYCTQGGVCGCEKGYTEIMRSSGFLDYCMKVPGSEDKKADVKNLSGKNRPVNSKIHDIFKGWSLQPLDPDGRVKMWVYGVSGGSFLIMIFLVFTSYLVCKKPKPHQSTPRHQKPLTLAYDGDLDM

Target ORF information:

RefSeq Version	NM_172485.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172485.3

ORF Insert Sequence:

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG CTGTCTCATG CATCTCTGAT 
GATAACCAGT CTGCAGAAAT GACAGAGTGC CTCAAGCAGA TGAATGGCAT GCCTCCCCTT 
GTGCAGGAAT GCACCATTCC CTGTCGGGAC GACTGCACCT TCACCCCTTG GTCTAAGTTC 
ACTCCCTGCT CCAAGAACTG TGAAGCAACT CAGATCAGGA GGCGGCAGCT AACAGGGAAA 
AGCAGGAAGA AAGAGAAGTG CCAGGATGCG TCCCTGTACC CTCTGGTGGA AATGGAACCA 
TGTCCCTGTG ATACGTTTAT GTCTCATCCT TATGGAAACT GGTCAGCTTG CATTCTACCA 
GAAGGCAAAA GGGATGCTCA GCAAGGAGGA TTGTGGGTGC AAGGAGATGA CAAAGAATGT 
GGAGAAGGCG TGCGCTTTCG AGCAATAGCA TGCTCCAATA TAAATGGAAG ACCGGTTGAC 
CCCTCATTCT GCAACAGCTC TGGTTACATT CAGGAAGCAT GTGTCATCCC CTGTCCGTTT 
GATTGCAAGC TAAGTGACTG GTCCAGCTGG GGTTCTTGCA GCTCATCTTG TGGGATTGGA 
GTGAGAATTC GATCAAAATG GCTAAAAGAA AAACCTTACA GTGGAGGTCG GCCATGTCCC 
AAACTGGATC TCAAGAATCA GGTTCATGAG GCAGTCCCAT GTTACAGTGA GTGTGATCAA 
TATTCCTGGG TCGTAGAACA CTGGTCTCCA TGCAAGATCA ATAATGAGCT GAGATCCCCA 
CGCTGTGGAA GAGGAACACA GTCTAGAAGA ATCAGGTGTG TGAGTACTGC TGACAGAGAA 
GGAGGGGCAG TGAACAGGAG CCTGTGCAAC CAGGATGATG CTCCCCAAGA AACCCAGGCC 
TGTTCTCTTC TGTGTCCCAG TGAGTGTGTC ATGTCCGAGT GGGGAACATG GAGCAGATGC 
CCACAGTCAT GTGACCCCCA TGCAATGCAG AGAAGAACTC GGCATCTGCT GAGGCCCTCA 
CTGAACTCAA GAACTTGTGG GGAAGACTCG CAGGTGCGGC CCTGCCTCCT CAATGAAAAC 
TGTTTCCAGT TCCAGTACAA TCTGACAGAG TGGAGCACAT GCCAGCTGAG TGAAAATGTT 
TCCTGTGGCC AGGGTGTGAG GACCCGCCTG CTGAGCTGTG TGCGCAGTGA TGGCAAGTCT 
GTCAGCATGG ACCACTGCGA GCAGCGTAAT CTGGAAAAAC CCCAGAGGAT GAGTATTCCC 
TGTCTGGTGG AATGTGTGGT CAACTGTCAG CTCTCAGGAT GGACAACATG GACAGAGTGT 
TCACAAACCT GTGGCCAAGG AGGTCGCATG AGCCGGACTC GATTTATCAT TATGCCAACC 
CAAGGAGAAG GTCGACAATG CCCCACAGAA CTCACCCAGC AGAAACCCTG CCCAGTGACA 
CCCTGCTATA GTTGGGTCCT TGGCAACTGG TCTGCATGTA AATTGGAGGG CGGAGATTGT 
GGAGAAGGAG TCCAGGTGCG CAGCTTCTCC TGTGTAGTCC ACAATGGCTC AATATCCCAC 
ACGGCTGTCC CTGTGGAGGA GGCCCTGTGT GGAGAAGTAC CCTTCCAAGA AGGAATCCTG 
AAGCAGCTGT GCTCTGTGCC TTGCCCAGGA GACTGCCACA TTACCCCATG GTCTGAGTGG 
AGCAAGTGTG AGCTAACCTG CATTGACGGA AGAAGCTTCG AGACCACAGG CCGCCAATCT 
AGGTCAAGGA CATTCATAAT CCAGTCTTTT GAGAATCAAG ATAGCTGCCC CCAACAGGTT 
CTAGAAACAC GACCATGTAC AGGTGGCAAA TGTTATCACT ACATATGGAA AGCAAGTCTT 
TGGAACAATA ATGAGCGGAC TGTGTGGTGC CAGCGCTCAG ATGGCCTTAA TGTCACAGGT 
GGCTGCTCCC CTCAGGCACG CCCTGCTGCT ATTCGGCAAT GCATTCCAGC ATGCAAAAAA 
CCTTTCTCCT ACTGCACACA GGGAGGAGTC TGTGGCTGTG AGAAGGGATA CACAGAGATA 
ATGAGATCAA GTGGCTTCCT GGATTACTGC ATGAAGGTGC CTGGCTCGGA GGATAAAAAA 
GCTGACGTGA AAAACCTCTC TGGGAAAAAT AGACCTGTGA ATTCCAAAAT ACATGATATT 
TTTAAAGGAT GGTCTCTCCA ACCTCTGGAC CCAGATGGCC GAGTAAAAAT GTGGGTTTAT 
GGCGTTTCCG GTGGCAGTTT TCTTATCATG ATCTTCCTAG TATTTACTTC CTATCTTGTT 
TGCAAGAAGC CAAAACCACA TCAAAGCACA CCTCGCCATC AGAAGCCACT GACCTTGGCC 
TACGATGGAG ACTTGGACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd7b ( XM_011247964.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246266.1 Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu72735
Clone ID Related Accession (Same CDS sequence)	XM_011247964.1 , XM_011247965.1 , XM_011247964.2
Accession Version	XM_011247964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 7B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG CTGTCTCATG CATCTCTGAT 
GATAACCAGT CTGCAGAAAT GACAGAGTGC CTCAAGCAGA TGAATGGCAT GCCTCCCCTT 
GTGCAGGAAT GCACCATTCC CTGTCGGGAC GACTGCACCT TCACCCCTTG GTCTAAGTTC 
ACTCCCTGCT CCAAGAACTG TGAAGCAACT CAGATCAGGA GGCGGCAGCT AACAGGGAAA 
AGCAGGAAGA AAGAGAAGTG CCAGGATGCG TCCCTGTACC CTCTGGTGGA AATGGAACCA 
TGTCCCTGTG ATACGTTTAT GTCTCATCCT TATGGAAACT GGTCAGCTTG CATTCTACCA 
GAAGGCAAAA GGGATGCTCA GCAAGGAGGA TTGTGGGTGC AAGGAGATGA CAAAGAATGT 
GGAGAAGGCG TGCGCTTTCG AGCAATAGCA TGCTCCAATA TAAATGGAAG ACCGGTTGAC 
CCCTCATTCT GCAACAGCTC TGGTTACATT CAGGAAGCAT GTGTCATCCC CTGTCCGTTT 
GATTGCAAGC TAAGTGACTG GTCCAGCTGG GGTTCTTGCA GCTCATCTTG TGGGATTGGA 
GTGAGAATTC GATCAAAATG GCTAAAAGAA AAACCTTACA GTGGAGGTCG GCCATGTCCC 
AAACTGGATC TCAAGAATCA GGCTCAGGTT CATGAGGCAG TCCCATGTTA CAGTGAGTGT 
GATCAATATT CCTGGGTCGT AGAACACTGG TCTCCATGCA AGATCAATAA TGAGCTGAGA 
TCCCCACGCT GTGGAAGAGG AACACAGTCT AGAAGAATCA GGTGTGTGAG TACTGCTGAC 
AGAGAAGGAG GGGCAGTGAA CAGGAGCCTG TGCAACCAGG ATGATGCTCC CCAAGAAACC 
CAGGCCTGTT CTCTTCTGTG TCCCAGTGAG TGTGTCATGT CCGAGTGGGG AACATGGAGC 
AGATGCCCAC AGTCATGTGA CCCCCATGCA ATGCAGAGAA GAACTCGGCA TCTGCTGAGG 
CCCTCACTGA ACTCAAGAAC TTGTGGGGAA GACTCGCAGG TGCGGCCCTG CCTCCTCAAT 
GAAAACTGTT TCCAGTTCCA GTACAATCTG ACAGAGTGGA GCACATGCCA GCTGAGTGAA 
AATGTTTCCT GTGGCCAGGG TGTGAGGACC CGCCTGCTGA GCTGTGTGCG CAGTGATGGC 
AAGTCTGTCA GCATGGACCA CTGCGAGCAG CGTAATCTGG AAAAACCCCA GAGGATGAGT 
ATTCCCTGTC TGGTGGAATG TGTGGTCAAC TGTCAGCTCT CAGGATGGAC AACATGGACA 
GAGTGTTCAC AAACCTGTGG CCAAGGAGGT CGCATGAGCC GGACTCGATT TATCATTATG 
CCAACCCAAG GAGAAGGTCG ACAATGCCCC ACAGAACTCA CCCAGCAGAA ACCCTGCCCA 
GTGACACCCT GCTATAGTTG GGTCCTTGGC AACTGGTCTG CATGTAAATT GGAGGGCGGA 
GATTGTGGAG AAGGAGTCCA GGTGCGCAGC TTCTCCTGTG TAGTCCACAA TGGCTCAATA 
TCCCACACGG CTGTCCCTGT GGAGGAGGCC CTGTGTGGAG AAGTACCCTT CCAAGAAGGA 
ATCCTGAAGC AGCTGTGCTC TGTGCCTTGC CCAGGAGACT GCCACATTAC CCCATGGTCT 
GAGTGGAGCA AGTGTGAGCT AACCTGCATT GACGGAAGAA GCTTCGAGAC CACAGGCCGC 
CAATCTAGGT CAAGGACATT CATAATCCAG TCTTTTGAGA ATCAAGATAG CTGCCCCCAA 
CAGGTTCTAG AAACACGACC ATGTACAGGT GGCAAATGTT ATCACTACAT ATGGAAAGCA 
AGTCTTTGGA ACAATAATGA GCGGACTGTG TGGTGCCAGC GCTCAGATGG CCTTAATGTC 
ACAGGTGGCT GCTCCCCTCA GGCACGCCCT GCTGCTATTC GGCAATGCAT TCCAGCATGC 
AAAAAACCTT TCTCCTACTG CACACAGGGA GGAGTCTGTG GCTGTGAGAA GGGATACACA 
GAGATAATGA GATCAAGTGG CTTCCTGGAT TACTGCATGA AGGTGCCTGG CTCGGAGGAT 
AAAAAAGCTG ACGTGAAAAA CCTCTCTGGG AAAAATAGAC CTGTGAATTC CAAAATACAT 
GATATTTTTA AAGGATGGTC TCTCCAACCT CTGGACCCAG ATGGCCGAGT AAAAATGTGG 
GTTTATGGCG TTTCCGGTGG CAGTTTTCTT ATCATGATCT TCCTAGTATT TACTTCCTAT 
CTTGTTTGCA AGAAGCCAAA ACCACATCAA AGCACACCTC GCCATCAGAA GCCACTGACC 
TTGGCCTACG ATGGAGACTT GGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246266.1
CDS	446..5275
Translation	MFLRSDLAVTHWVSRSMRKLFLVLSLLLSQAAHLEGRKDNQFLWKTGPWGRCAGDCGPGGAQSRAVWCFHIEGWTSPMSNCDESSQPPKERSCFRVCDWHSDLFQWEVSDWHRCLLVPGAQGEPRPRAVECVTAQHGLQHRTVRCLQKLNRTMVSNEICEHFAPQPPTEQACLIPCPRDCVVSEFSPWSTCPEGCGKKLQHRTRVAIAPPLYGGLQCPNLTESRACEAPVSCPLGKEEYSFSLKVGPWSKCRLPHLKEVDLSGRNIQDFSSDSNEQVTLTHQSYKAHHHSQPGDVVIGFQTRQVWCTRSDGRNALLSLCVRDSFPLTVQPCVMPKDCETSEWSPWSPCSKTCRSGTLSPGVRSRSRNVKHIAIGGGQECPELLEKETCIAEGEFLQPCPRYSWRTSEWKECQVSLLLEQHDPLWHETGPICGGGIQTREVYCAQSLPATIASRTKEVSRPVERTLCLGPAPSASQLCNVPCSMDCIVSSWSTWGPCVFENCHDPQGKKGFRMRQRHVLMESTGPMGRCPHLAESVPCEDPMCHRWLASEGICIADHGKCGLGHRILKAVCQNERGEEVSGGLCPVPPPPERMACEIPCRMDCVVSEWTVWSSCSQSCSNKNSDGKQTRSRSILALAGEGGKTCPSSQELQEYRLCNDHSCTQLYWETSAWGSCSENTLVTALNVTIGWNGEATCGVGIQTRKVFCIKSHVGQVMTKRCPESTRPETVRPCFLPCKKDCLVTAFSEWTPCPRPCQPGNTTIKQSRYRIIIQEAANGGQECPDTLFEERECEDISLCPSYRWKPQKWSSCILVPESIRQGRTGTSEACGKGLQTRAVSCISDDNQSAEMTECLKQMNGMPPLVQECTIPCRDDCTFTPWSKFTPCSKNCEATQIRRRQLTGKSRKKEKCQDASLYPLVEMEPCPCDTFMSHPYGNWSACILPEGKRDAQQGGLWVQGDDKECGEGVRFRAIACSNINGRPVDPSFCNSSGYIQEACVIPCPFDCKLSDWSSWGSCSSSCGIGVRIRSKWLKEKPYSGGRPCPKLDLKNQAQVHEAVPCYSECDQYSWVVEHWSPCKINNELRSPRCGRGTQSRRIRCVSTADREGGAVNRSLCNQDDAPQETQACSLLCPSECVMSEWGTWSRCPQSCDPHAMQRRTRHLLRPSLNSRTCGEDSQVRPCLLNENCFQFQYNLTEWSTCQLSENVSCGQGVRTRLLSCVRSDGKSVSMDHCEQRNLEKPQRMSIPCLVECVVNCQLSGWTTWTECSQTCGQGGRMSRTRFIIMPTQGEGRQCPTELTQQKPCPVTPCYSWVLGNWSACKLEGGDCGEGVQVRSFSCVVHNGSISHTAVPVEEALCGEVPFQEGILKQLCSVPCPGDCHITPWSEWSKCELTCIDGRSFETTGRQSRSRTFIIQSFENQDSCPQQVLETRPCTGGKCYHYIWKASLWNNNERTVWCQRSDGLNVTGGCSPQARPAAIRQCIPACKKPFSYCTQGGVCGCEKGYTEIMRSSGFLDYCMKVPGSEDKKADVKNLSGKNRPVNSKIHDIFKGWSLQPLDPDGRVKMWVYGVSGGSFLIMIFLVFTSYLVCKKPKPHQSTPRHQKPLTLAYDGDLDM

Target ORF information:

RefSeq Version	XM_011247964.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247964.2

ORF Insert Sequence:

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG CTGTCTCATG CATCTCTGAT 
GATAACCAGT CTGCAGAAAT GACAGAGTGC CTCAAGCAGA TGAATGGCAT GCCTCCCCTT 
GTGCAGGAAT GCACCATTCC CTGTCGGGAC GACTGCACCT TCACCCCTTG GTCTAAGTTC 
ACTCCCTGCT CCAAGAACTG TGAAGCAACT CAGATCAGGA GGCGGCAGCT AACAGGGAAA 
AGCAGGAAGA AAGAGAAGTG CCAGGATGCG TCCCTGTACC CTCTGGTGGA AATGGAACCA 
TGTCCCTGTG ATACGTTTAT GTCTCATCCT TATGGAAACT GGTCAGCTTG CATTCTACCA 
GAAGGCAAAA GGGATGCTCA GCAAGGAGGA TTGTGGGTGC AAGGAGATGA CAAAGAATGT 
GGAGAAGGCG TGCGCTTTCG AGCAATAGCA TGCTCCAATA TAAATGGAAG ACCGGTTGAC 
CCCTCATTCT GCAACAGCTC TGGTTACATT CAGGAAGCAT GTGTCATCCC CTGTCCGTTT 
GATTGCAAGC TAAGTGACTG GTCCAGCTGG GGTTCTTGCA GCTCATCTTG TGGGATTGGA 
GTGAGAATTC GATCAAAATG GCTAAAAGAA AAACCTTACA GTGGAGGTCG GCCATGTCCC 
AAACTGGATC TCAAGAATCA GGCTCAGGTT CATGAGGCAG TCCCATGTTA CAGTGAGTGT 
GATCAATATT CCTGGGTCGT AGAACACTGG TCTCCATGCA AGATCAATAA TGAGCTGAGA 
TCCCCACGCT GTGGAAGAGG AACACAGTCT AGAAGAATCA GGTGTGTGAG TACTGCTGAC 
AGAGAAGGAG GGGCAGTGAA CAGGAGCCTG TGCAACCAGG ATGATGCTCC CCAAGAAACC 
CAGGCCTGTT CTCTTCTGTG TCCCAGTGAG TGTGTCATGT CCGAGTGGGG AACATGGAGC 
AGATGCCCAC AGTCATGTGA CCCCCATGCA ATGCAGAGAA GAACTCGGCA TCTGCTGAGG 
CCCTCACTGA ACTCAAGAAC TTGTGGGGAA GACTCGCAGG TGCGGCCCTG CCTCCTCAAT 
GAAAACTGTT TCCAGTTCCA GTACAATCTG ACAGAGTGGA GCACATGCCA GCTGAGTGAA 
AATGTTTCCT GTGGCCAGGG TGTGAGGACC CGCCTGCTGA GCTGTGTGCG CAGTGATGGC 
AAGTCTGTCA GCATGGACCA CTGCGAGCAG CGTAATCTGG AAAAACCCCA GAGGATGAGT 
ATTCCCTGTC TGGTGGAATG TGTGGTCAAC TGTCAGCTCT CAGGATGGAC AACATGGACA 
GAGTGTTCAC AAACCTGTGG CCAAGGAGGT CGCATGAGCC GGACTCGATT TATCATTATG 
CCAACCCAAG GAGAAGGTCG ACAATGCCCC ACAGAACTCA CCCAGCAGAA ACCCTGCCCA 
GTGACACCCT GCTATAGTTG GGTCCTTGGC AACTGGTCTG CATGTAAATT GGAGGGCGGA 
GATTGTGGAG AAGGAGTCCA GGTGCGCAGC TTCTCCTGTG TAGTCCACAA TGGCTCAATA 
TCCCACACGG CTGTCCCTGT GGAGGAGGCC CTGTGTGGAG AAGTACCCTT CCAAGAAGGA 
ATCCTGAAGC AGCTGTGCTC TGTGCCTTGC CCAGGAGACT GCCACATTAC CCCATGGTCT 
GAGTGGAGCA AGTGTGAGCT AACCTGCATT GACGGAAGAA GCTTCGAGAC CACAGGCCGC 
CAATCTAGGT CAAGGACATT CATAATCCAG TCTTTTGAGA ATCAAGATAG CTGCCCCCAA 
CAGGTTCTAG AAACACGACC ATGTACAGGT GGCAAATGTT ATCACTACAT ATGGAAAGCA 
AGTCTTTGGA ACAATAATGA GCGGACTGTG TGGTGCCAGC GCTCAGATGG CCTTAATGTC 
ACAGGTGGCT GCTCCCCTCA GGCACGCCCT GCTGCTATTC GGCAATGCAT TCCAGCATGC 
AAAAAACCTT TCTCCTACTG CACACAGGGA GGAGTCTGTG GCTGTGAGAA GGGATACACA 
GAGATAATGA GATCAAGTGG CTTCCTGGAT TACTGCATGA AGGTGCCTGG CTCGGAGGAT 
AAAAAAGCTG ACGTGAAAAA CCTCTCTGGG AAAAATAGAC CTGTGAATTC CAAAATACAT 
GATATTTTTA AAGGATGGTC TCTCCAACCT CTGGACCCAG ATGGCCGAGT AAAAATGTGG 
GTTTATGGCG TTTCCGGTGG CAGTTTTCTT ATCATGATCT TCCTAGTATT TACTTCCTAT 
CTTGTTTGCA AGAAGCCAAA ACCACATCAA AGCACACCTC GCCATCAGAA GCCACTGACC 
TTGGCCTACG ATGGAGACTT GGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd7b ( XM_011247964.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246266.1 Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X1, mRNA.

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CloneID	OMu72735
Clone ID Related Accession (Same CDS sequence)	XM_011247964.1 , XM_011247965.1 , XM_011247964.2
Accession Version	XM_011247964.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 7B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG CTGTCTCATG CATCTCTGAT 
GATAACCAGT CTGCAGAAAT GACAGAGTGC CTCAAGCAGA TGAATGGCAT GCCTCCCCTT 
GTGCAGGAAT GCACCATTCC CTGTCGGGAC GACTGCACCT TCACCCCTTG GTCTAAGTTC 
ACTCCCTGCT CCAAGAACTG TGAAGCAACT CAGATCAGGA GGCGGCAGCT AACAGGGAAA 
AGCAGGAAGA AAGAGAAGTG CCAGGATGCG TCCCTGTACC CTCTGGTGGA AATGGAACCA 
TGTCCCTGTG ATACGTTTAT GTCTCATCCT TATGGAAACT GGTCAGCTTG CATTCTACCA 
GAAGGCAAAA GGGATGCTCA GCAAGGAGGA TTGTGGGTGC AAGGAGATGA CAAAGAATGT 
GGAGAAGGCG TGCGCTTTCG AGCAATAGCA TGCTCCAATA TAAATGGAAG ACCGGTTGAC 
CCCTCATTCT GCAACAGCTC TGGTTACATT CAGGAAGCAT GTGTCATCCC CTGTCCGTTT 
GATTGCAAGC TAAGTGACTG GTCCAGCTGG GGTTCTTGCA GCTCATCTTG TGGGATTGGA 
GTGAGAATTC GATCAAAATG GCTAAAAGAA AAACCTTACA GTGGAGGTCG GCCATGTCCC 
AAACTGGATC TCAAGAATCA GGCTCAGGTT CATGAGGCAG TCCCATGTTA CAGTGAGTGT 
GATCAATATT CCTGGGTCGT AGAACACTGG TCTCCATGCA AGATCAATAA TGAGCTGAGA 
TCCCCACGCT GTGGAAGAGG AACACAGTCT AGAAGAATCA GGTGTGTGAG TACTGCTGAC 
AGAGAAGGAG GGGCAGTGAA CAGGAGCCTG TGCAACCAGG ATGATGCTCC CCAAGAAACC 
CAGGCCTGTT CTCTTCTGTG TCCCAGTGAG TGTGTCATGT CCGAGTGGGG AACATGGAGC 
AGATGCCCAC AGTCATGTGA CCCCCATGCA ATGCAGAGAA GAACTCGGCA TCTGCTGAGG 
CCCTCACTGA ACTCAAGAAC TTGTGGGGAA GACTCGCAGG TGCGGCCCTG CCTCCTCAAT 
GAAAACTGTT TCCAGTTCCA GTACAATCTG ACAGAGTGGA GCACATGCCA GCTGAGTGAA 
AATGTTTCCT GTGGCCAGGG TGTGAGGACC CGCCTGCTGA GCTGTGTGCG CAGTGATGGC 
AAGTCTGTCA GCATGGACCA CTGCGAGCAG CGTAATCTGG AAAAACCCCA GAGGATGAGT 
ATTCCCTGTC TGGTGGAATG TGTGGTCAAC TGTCAGCTCT CAGGATGGAC AACATGGACA 
GAGTGTTCAC AAACCTGTGG CCAAGGAGGT CGCATGAGCC GGACTCGATT TATCATTATG 
CCAACCCAAG GAGAAGGTCG ACAATGCCCC ACAGAACTCA CCCAGCAGAA ACCCTGCCCA 
GTGACACCCT GCTATAGTTG GGTCCTTGGC AACTGGTCTG CATGTAAATT GGAGGGCGGA 
GATTGTGGAG AAGGAGTCCA GGTGCGCAGC TTCTCCTGTG TAGTCCACAA TGGCTCAATA 
TCCCACACGG CTGTCCCTGT GGAGGAGGCC CTGTGTGGAG AAGTACCCTT CCAAGAAGGA 
ATCCTGAAGC AGCTGTGCTC TGTGCCTTGC CCAGGAGACT GCCACATTAC CCCATGGTCT 
GAGTGGAGCA AGTGTGAGCT AACCTGCATT GACGGAAGAA GCTTCGAGAC CACAGGCCGC 
CAATCTAGGT CAAGGACATT CATAATCCAG TCTTTTGAGA ATCAAGATAG CTGCCCCCAA 
CAGGTTCTAG AAACACGACC ATGTACAGGT GGCAAATGTT ATCACTACAT ATGGAAAGCA 
AGTCTTTGGA ACAATAATGA GCGGACTGTG TGGTGCCAGC GCTCAGATGG CCTTAATGTC 
ACAGGTGGCT GCTCCCCTCA GGCACGCCCT GCTGCTATTC GGCAATGCAT TCCAGCATGC 
AAAAAACCTT TCTCCTACTG CACACAGGGA GGAGTCTGTG GCTGTGAGAA GGGATACACA 
GAGATAATGA GATCAAGTGG CTTCCTGGAT TACTGCATGA AGGTGCCTGG CTCGGAGGAT 
AAAAAAGCTG ACGTGAAAAA CCTCTCTGGG AAAAATAGAC CTGTGAATTC CAAAATACAT 
GATATTTTTA AAGGATGGTC TCTCCAACCT CTGGACCCAG ATGGCCGAGT AAAAATGTGG 
GTTTATGGCG TTTCCGGTGG CAGTTTTCTT ATCATGATCT TCCTAGTATT TACTTCCTAT 
CTTGTTTGCA AGAAGCCAAA ACCACATCAA AGCACACCTC GCCATCAGAA GCCACTGACC 
TTGGCCTACG ATGGAGACTT GGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246266.1
CDS	173..5002
Translation	MFLRSDLAVTHWVSRSMRKLFLVLSLLLSQAAHLEGRKDNQFLWKTGPWGRCAGDCGPGGAQSRAVWCFHIEGWTSPMSNCDESSQPPKERSCFRVCDWHSDLFQWEVSDWHRCLLVPGAQGEPRPRAVECVTAQHGLQHRTVRCLQKLNRTMVSNEICEHFAPQPPTEQACLIPCPRDCVVSEFSPWSTCPEGCGKKLQHRTRVAIAPPLYGGLQCPNLTESRACEAPVSCPLGKEEYSFSLKVGPWSKCRLPHLKEVDLSGRNIQDFSSDSNEQVTLTHQSYKAHHHSQPGDVVIGFQTRQVWCTRSDGRNALLSLCVRDSFPLTVQPCVMPKDCETSEWSPWSPCSKTCRSGTLSPGVRSRSRNVKHIAIGGGQECPELLEKETCIAEGEFLQPCPRYSWRTSEWKECQVSLLLEQHDPLWHETGPICGGGIQTREVYCAQSLPATIASRTKEVSRPVERTLCLGPAPSASQLCNVPCSMDCIVSSWSTWGPCVFENCHDPQGKKGFRMRQRHVLMESTGPMGRCPHLAESVPCEDPMCHRWLASEGICIADHGKCGLGHRILKAVCQNERGEEVSGGLCPVPPPPERMACEIPCRMDCVVSEWTVWSSCSQSCSNKNSDGKQTRSRSILALAGEGGKTCPSSQELQEYRLCNDHSCTQLYWETSAWGSCSENTLVTALNVTIGWNGEATCGVGIQTRKVFCIKSHVGQVMTKRCPESTRPETVRPCFLPCKKDCLVTAFSEWTPCPRPCQPGNTTIKQSRYRIIIQEAANGGQECPDTLFEERECEDISLCPSYRWKPQKWSSCILVPESIRQGRTGTSEACGKGLQTRAVSCISDDNQSAEMTECLKQMNGMPPLVQECTIPCRDDCTFTPWSKFTPCSKNCEATQIRRRQLTGKSRKKEKCQDASLYPLVEMEPCPCDTFMSHPYGNWSACILPEGKRDAQQGGLWVQGDDKECGEGVRFRAIACSNINGRPVDPSFCNSSGYIQEACVIPCPFDCKLSDWSSWGSCSSSCGIGVRIRSKWLKEKPYSGGRPCPKLDLKNQAQVHEAVPCYSECDQYSWVVEHWSPCKINNELRSPRCGRGTQSRRIRCVSTADREGGAVNRSLCNQDDAPQETQACSLLCPSECVMSEWGTWSRCPQSCDPHAMQRRTRHLLRPSLNSRTCGEDSQVRPCLLNENCFQFQYNLTEWSTCQLSENVSCGQGVRTRLLSCVRSDGKSVSMDHCEQRNLEKPQRMSIPCLVECVVNCQLSGWTTWTECSQTCGQGGRMSRTRFIIMPTQGEGRQCPTELTQQKPCPVTPCYSWVLGNWSACKLEGGDCGEGVQVRSFSCVVHNGSISHTAVPVEEALCGEVPFQEGILKQLCSVPCPGDCHITPWSEWSKCELTCIDGRSFETTGRQSRSRTFIIQSFENQDSCPQQVLETRPCTGGKCYHYIWKASLWNNNERTVWCQRSDGLNVTGGCSPQARPAAIRQCIPACKKPFSYCTQGGVCGCEKGYTEIMRSSGFLDYCMKVPGSEDKKADVKNLSGKNRPVNSKIHDIFKGWSLQPLDPDGRVKMWVYGVSGGSFLIMIFLVFTSYLVCKKPKPHQSTPRHQKPLTLAYDGDLDM

Target ORF information:

RefSeq Version	XM_011247964.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247964.1

ORF Insert Sequence:

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG CTGTCTCATG CATCTCTGAT 
GATAACCAGT CTGCAGAAAT GACAGAGTGC CTCAAGCAGA TGAATGGCAT GCCTCCCCTT 
GTGCAGGAAT GCACCATTCC CTGTCGGGAC GACTGCACCT TCACCCCTTG GTCTAAGTTC 
ACTCCCTGCT CCAAGAACTG TGAAGCAACT CAGATCAGGA GGCGGCAGCT AACAGGGAAA 
AGCAGGAAGA AAGAGAAGTG CCAGGATGCG TCCCTGTACC CTCTGGTGGA AATGGAACCA 
TGTCCCTGTG ATACGTTTAT GTCTCATCCT TATGGAAACT GGTCAGCTTG CATTCTACCA 
GAAGGCAAAA GGGATGCTCA GCAAGGAGGA TTGTGGGTGC AAGGAGATGA CAAAGAATGT 
GGAGAAGGCG TGCGCTTTCG AGCAATAGCA TGCTCCAATA TAAATGGAAG ACCGGTTGAC 
CCCTCATTCT GCAACAGCTC TGGTTACATT CAGGAAGCAT GTGTCATCCC CTGTCCGTTT 
GATTGCAAGC TAAGTGACTG GTCCAGCTGG GGTTCTTGCA GCTCATCTTG TGGGATTGGA 
GTGAGAATTC GATCAAAATG GCTAAAAGAA AAACCTTACA GTGGAGGTCG GCCATGTCCC 
AAACTGGATC TCAAGAATCA GGCTCAGGTT CATGAGGCAG TCCCATGTTA CAGTGAGTGT 
GATCAATATT CCTGGGTCGT AGAACACTGG TCTCCATGCA AGATCAATAA TGAGCTGAGA 
TCCCCACGCT GTGGAAGAGG AACACAGTCT AGAAGAATCA GGTGTGTGAG TACTGCTGAC 
AGAGAAGGAG GGGCAGTGAA CAGGAGCCTG TGCAACCAGG ATGATGCTCC CCAAGAAACC 
CAGGCCTGTT CTCTTCTGTG TCCCAGTGAG TGTGTCATGT CCGAGTGGGG AACATGGAGC 
AGATGCCCAC AGTCATGTGA CCCCCATGCA ATGCAGAGAA GAACTCGGCA TCTGCTGAGG 
CCCTCACTGA ACTCAAGAAC TTGTGGGGAA GACTCGCAGG TGCGGCCCTG CCTCCTCAAT 
GAAAACTGTT TCCAGTTCCA GTACAATCTG ACAGAGTGGA GCACATGCCA GCTGAGTGAA 
AATGTTTCCT GTGGCCAGGG TGTGAGGACC CGCCTGCTGA GCTGTGTGCG CAGTGATGGC 
AAGTCTGTCA GCATGGACCA CTGCGAGCAG CGTAATCTGG AAAAACCCCA GAGGATGAGT 
ATTCCCTGTC TGGTGGAATG TGTGGTCAAC TGTCAGCTCT CAGGATGGAC AACATGGACA 
GAGTGTTCAC AAACCTGTGG CCAAGGAGGT CGCATGAGCC GGACTCGATT TATCATTATG 
CCAACCCAAG GAGAAGGTCG ACAATGCCCC ACAGAACTCA CCCAGCAGAA ACCCTGCCCA 
GTGACACCCT GCTATAGTTG GGTCCTTGGC AACTGGTCTG CATGTAAATT GGAGGGCGGA 
GATTGTGGAG AAGGAGTCCA GGTGCGCAGC TTCTCCTGTG TAGTCCACAA TGGCTCAATA 
TCCCACACGG CTGTCCCTGT GGAGGAGGCC CTGTGTGGAG AAGTACCCTT CCAAGAAGGA 
ATCCTGAAGC AGCTGTGCTC TGTGCCTTGC CCAGGAGACT GCCACATTAC CCCATGGTCT 
GAGTGGAGCA AGTGTGAGCT AACCTGCATT GACGGAAGAA GCTTCGAGAC CACAGGCCGC 
CAATCTAGGT CAAGGACATT CATAATCCAG TCTTTTGAGA ATCAAGATAG CTGCCCCCAA 
CAGGTTCTAG AAACACGACC ATGTACAGGT GGCAAATGTT ATCACTACAT ATGGAAAGCA 
AGTCTTTGGA ACAATAATGA GCGGACTGTG TGGTGCCAGC GCTCAGATGG CCTTAATGTC 
ACAGGTGGCT GCTCCCCTCA GGCACGCCCT GCTGCTATTC GGCAATGCAT TCCAGCATGC 
AAAAAACCTT TCTCCTACTG CACACAGGGA GGAGTCTGTG GCTGTGAGAA GGGATACACA 
GAGATAATGA GATCAAGTGG CTTCCTGGAT TACTGCATGA AGGTGCCTGG CTCGGAGGAT 
AAAAAAGCTG ACGTGAAAAA CCTCTCTGGG AAAAATAGAC CTGTGAATTC CAAAATACAT 
GATATTTTTA AAGGATGGTC TCTCCAACCT CTGGACCCAG ATGGCCGAGT AAAAATGTGG 
GTTTATGGCG TTTCCGGTGG CAGTTTTCTT ATCATGATCT TCCTAGTATT TACTTCCTAT 
CTTGTTTGCA AGAAGCCAAA ACCACATCAA AGCACACCTC GCCATCAGAA GCCACTGACC 
TTGGCCTACG ATGGAGACTT GGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd7b ( XM_011247965.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246267.1 Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X2, mRNA.

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CloneID	OMu72735
Clone ID Related Accession (Same CDS sequence)	XM_011247964.1 , XM_011247965.1 , XM_011247964.2
Accession Version	XM_011247965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 7B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG CTGTCTCATG CATCTCTGAT 
GATAACCAGT CTGCAGAAAT GACAGAGTGC CTCAAGCAGA TGAATGGCAT GCCTCCCCTT 
GTGCAGGAAT GCACCATTCC CTGTCGGGAC GACTGCACCT TCACCCCTTG GTCTAAGTTC 
ACTCCCTGCT CCAAGAACTG TGAAGCAACT CAGATCAGGA GGCGGCAGCT AACAGGGAAA 
AGCAGGAAGA AAGAGAAGTG CCAGGATGCG TCCCTGTACC CTCTGGTGGA AATGGAACCA 
TGTCCCTGTG ATACGTTTAT GTCTCATCCT TATGGAAACT GGTCAGCTTG CATTCTACCA 
GAAGGCAAAA GGGATGCTCA GCAAGGAGGA TTGTGGGTGC AAGGAGATGA CAAAGAATGT 
GGAGAAGGCG TGCGCTTTCG AGCAATAGCA TGCTCCAATA TAAATGGAAG ACCGGTTGAC 
CCCTCATTCT GCAACAGCTC TGGTTACATT CAGGAAGCAT GTGTCATCCC CTGTCCGTTT 
GATTGCAAGC TAAGTGACTG GTCCAGCTGG GGTTCTTGCA GCTCATCTTG TGGGATTGGA 
GTGAGAATTC GATCAAAATG GCTAAAAGAA AAACCTTACA GTGGAGGTCG GCCATGTCCC 
AAACTGGATC TCAAGAATCA GGCTCAGGTT CATGAGGCAG TCCCATGTTA CAGTGAGTGT 
GATCAATATT CCTGGGTCGT AGAACACTGG TCTCCATGCA AGATCAATAA TGAGCTGAGA 
TCCCCACGCT GTGGAAGAGG AACACAGTCT AGAAGAATCA GGTGTGTGAG TACTGCTGAC 
AGAGAAGGAG GGGCAGTGAA CAGGAGCCTG TGCAACCAGG ATGATGCTCC CCAAGAAACC 
CAGGCCTGTT CTCTTCTGTG TCCCAGTGAG TGTGTCATGT CCGAGTGGGG AACATGGAGC 
AGATGCCCAC AGTCATGTGA CCCCCATGCA ATGCAGAGAA GAACTCGGCA TCTGCTGAGG 
CCCTCACTGA ACTCAAGAAC TTGTGGGGAA GACTCGCAGG TGCGGCCCTG CCTCCTCAAT 
GAAAACTGTT TCCAGTTCCA GTACAATCTG ACAGAGTGGA GCACATGCCA GCTGAGTGAA 
AATGTTTCCT GTGGCCAGGG TGTGAGGACC CGCCTGCTGA GCTGTGTGCG CAGTGATGGC 
AAGTCTGTCA GCATGGACCA CTGCGAGCAG CGTAATCTGG AAAAACCCCA GAGGATGAGT 
ATTCCCTGTC TGGTGGAATG TGTGGTCAAC TGTCAGCTCT CAGGATGGAC AACATGGACA 
GAGTGTTCAC AAACCTGTGG CCAAGGAGGT CGCATGAGCC GGACTCGATT TATCATTATG 
CCAACCCAAG GAGAAGGTCG ACAATGCCCC ACAGAACTCA CCCAGCAGAA ACCCTGCCCA 
GTGACACCCT GCTATAGTTG GGTCCTTGGC AACTGGTCTG CATGTAAATT GGAGGGCGGA 
GATTGTGGAG AAGGAGTCCA GGTGCGCAGC TTCTCCTGTG TAGTCCACAA TGGCTCAATA 
TCCCACACGG CTGTCCCTGT GGAGGAGGCC CTGTGTGGAG AAGTACCCTT CCAAGAAGGA 
ATCCTGAAGC AGCTGTGCTC TGTGCCTTGC CCAGGAGACT GCCACATTAC CCCATGGTCT 
GAGTGGAGCA AGTGTGAGCT AACCTGCATT GACGGAAGAA GCTTCGAGAC CACAGGCCGC 
CAATCTAGGT CAAGGACATT CATAATCCAG TCTTTTGAGA ATCAAGATAG CTGCCCCCAA 
CAGGTTCTAG AAACACGACC ATGTACAGGT GGCAAATGTT ATCACTACAT ATGGAAAGCA 
AGTCTTTGGA ACAATAATGA GCGGACTGTG TGGTGCCAGC GCTCAGATGG CCTTAATGTC 
ACAGGTGGCT GCTCCCCTCA GGCACGCCCT GCTGCTATTC GGCAATGCAT TCCAGCATGC 
AAAAAACCTT TCTCCTACTG CACACAGGGA GGAGTCTGTG GCTGTGAGAA GGGATACACA 
GAGATAATGA GATCAAGTGG CTTCCTGGAT TACTGCATGA AGGTGCCTGG CTCGGAGGAT 
AAAAAAGCTG ACGTGAAAAA CCTCTCTGGG AAAAATAGAC CTGTGAATTC CAAAATACAT 
GATATTTTTA AAGGATGGTC TCTCCAACCT CTGGACCCAG ATGGCCGAGT AAAAATGTGG 
GTTTATGGCG TTTCCGGTGG CAGTTTTCTT ATCATGATCT TCCTAGTATT TACTTCCTAT 
CTTGTTTGCA AGAAGCCAAA ACCACATCAA AGCACACCTC GCCATCAGAA GCCACTGACC 
TTGGCCTACG ATGGAGACTT GGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246267.1
CDS	535..5364
Translation	MFLRSDLAVTHWVSRSMRKLFLVLSLLLSQAAHLEGRKDNQFLWKTGPWGRCAGDCGPGGAQSRAVWCFHIEGWTSPMSNCDESSQPPKERSCFRVCDWHSDLFQWEVSDWHRCLLVPGAQGEPRPRAVECVTAQHGLQHRTVRCLQKLNRTMVSNEICEHFAPQPPTEQACLIPCPRDCVVSEFSPWSTCPEGCGKKLQHRTRVAIAPPLYGGLQCPNLTESRACEAPVSCPLGKEEYSFSLKVGPWSKCRLPHLKEVDLSGRNIQDFSSDSNEQVTLTHQSYKAHHHSQPGDVVIGFQTRQVWCTRSDGRNALLSLCVRDSFPLTVQPCVMPKDCETSEWSPWSPCSKTCRSGTLSPGVRSRSRNVKHIAIGGGQECPELLEKETCIAEGEFLQPCPRYSWRTSEWKECQVSLLLEQHDPLWHETGPICGGGIQTREVYCAQSLPATIASRTKEVSRPVERTLCLGPAPSASQLCNVPCSMDCIVSSWSTWGPCVFENCHDPQGKKGFRMRQRHVLMESTGPMGRCPHLAESVPCEDPMCHRWLASEGICIADHGKCGLGHRILKAVCQNERGEEVSGGLCPVPPPPERMACEIPCRMDCVVSEWTVWSSCSQSCSNKNSDGKQTRSRSILALAGEGGKTCPSSQELQEYRLCNDHSCTQLYWETSAWGSCSENTLVTALNVTIGWNGEATCGVGIQTRKVFCIKSHVGQVMTKRCPESTRPETVRPCFLPCKKDCLVTAFSEWTPCPRPCQPGNTTIKQSRYRIIIQEAANGGQECPDTLFEERECEDISLCPSYRWKPQKWSSCILVPESIRQGRTGTSEACGKGLQTRAVSCISDDNQSAEMTECLKQMNGMPPLVQECTIPCRDDCTFTPWSKFTPCSKNCEATQIRRRQLTGKSRKKEKCQDASLYPLVEMEPCPCDTFMSHPYGNWSACILPEGKRDAQQGGLWVQGDDKECGEGVRFRAIACSNINGRPVDPSFCNSSGYIQEACVIPCPFDCKLSDWSSWGSCSSSCGIGVRIRSKWLKEKPYSGGRPCPKLDLKNQAQVHEAVPCYSECDQYSWVVEHWSPCKINNELRSPRCGRGTQSRRIRCVSTADREGGAVNRSLCNQDDAPQETQACSLLCPSECVMSEWGTWSRCPQSCDPHAMQRRTRHLLRPSLNSRTCGEDSQVRPCLLNENCFQFQYNLTEWSTCQLSENVSCGQGVRTRLLSCVRSDGKSVSMDHCEQRNLEKPQRMSIPCLVECVVNCQLSGWTTWTECSQTCGQGGRMSRTRFIIMPTQGEGRQCPTELTQQKPCPVTPCYSWVLGNWSACKLEGGDCGEGVQVRSFSCVVHNGSISHTAVPVEEALCGEVPFQEGILKQLCSVPCPGDCHITPWSEWSKCELTCIDGRSFETTGRQSRSRTFIIQSFENQDSCPQQVLETRPCTGGKCYHYIWKASLWNNNERTVWCQRSDGLNVTGGCSPQARPAAIRQCIPACKKPFSYCTQGGVCGCEKGYTEIMRSSGFLDYCMKVPGSEDKKADVKNLSGKNRPVNSKIHDIFKGWSLQPLDPDGRVKMWVYGVSGGSFLIMIFLVFTSYLVCKKPKPHQSTPRHQKPLTLAYDGDLDM

Target ORF information:

RefSeq Version	XM_011247965.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 7B (Thsd7b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247965.1

ORF Insert Sequence:

ATGTTTCTGA GGAGTGATCT GGCTGTCACT CACTGGGTTT CCAGGAGCAT GAGGAAGCTC 
TTTCTAGTGC TTTCTCTCCT GCTGTCCCAA GCAGCTCATT TGGAAGGCAG GAAGGACAAT 
CAGTTCCTCT GGAAAACCGG TCCCTGGGGA AGGTGTGCAG GTGACTGCGG ACCAGGCGGA 
GCCCAAAGTC GTGCTGTATG GTGCTTTCAC ATAGAAGGGT GGACGAGTCC CATGTCGAAC 
TGTGATGAGA GCAGCCAACC TCCAAAGGAA AGGAGCTGTT TCCGCGTGTG TGACTGGCAC 
AGTGACCTGT TCCAGTGGGA GGTTTCCGAC TGGCATCGCT GCCTTCTGGT TCCTGGGGCC 
CAAGGTGAGC CCAGGCCTCG GGCTGTGGAA TGTGTGACTG CACAGCACGG TCTACAGCAC 
CGGACAGTGC GCTGTCTTCA GAAGCTCAAT AGAACCATGG TGTCCAATGA GATCTGTGAA 
CACTTTGCTC CTCAGCCCCC CACAGAGCAA GCCTGCCTCA TTCCTTGCCC CAGGGACTGC 
GTGGTATCTG AGTTCTCCCC ATGGTCCACC TGCCCCGAAG GATGTGGGAA GAAACTGCAG 
CATAGAACTC GTGTGGCCAT CGCGCCCCCT CTGTATGGAG GTCTGCAGTG TCCAAATCTC 
ACGGAGTCCA GAGCCTGCGA GGCTCCAGTT TCCTGTCCTC TTGGGAAAGA AGAATATTCC 
TTCAGCCTAA AGGTGGGACC ATGGAGCAAA TGTAGACTCC CTCACCTTAA GGAAGTCGAC 
CTCAGCGGAA GAAATATCCA GGATTTTAGC TCTGATTCAA ACGAGCAAGT CACCCTAACA 
CATCAGAGTT ACAAAGCACA CCACCACTCC CAGCCTGGGG ATGTAGTGAT AGGGTTTCAA 
ACCAGGCAGG TGTGGTGTAC CAGAAGTGAT GGAAGAAACG CCCTGTTAAG CCTTTGTGTT 
CGTGATTCCT TCCCTCTGAC TGTCCAGCCT TGTGTGATGC CAAAAGACTG TGAGACTTCT 
GAATGGTCTC CATGGAGCCC CTGTTCCAAG ACCTGCCGTT CTGGGACTTT ATCACCTGGA 
GTTAGAAGCA GGAGCCGGAA CGTGAAGCAT ATTGCTATTG GAGGTGGCCA GGAATGCCCA 
GAACTTCTTG AGAAAGAGAC CTGCATTGCT GAAGGGGAAT TTCTGCAACC ATGTCCCAGG 
TATTCCTGGA GAACCTCTGA ATGGAAGGAA TGCCAAGTCT CTCTTCTTCT GGAGCAACAC 
GATCCACTTT GGCATGAGAC AGGACCAATC TGTGGTGGTG GGATCCAGAC CCGGGAGGTG 
TACTGTGCCC AGAGCCTACC AGCAACCATT GCATCAAGGA CCAAGGAAGT CTCCAGGCCC 
GTGGAAAGGA CACTATGCTT GGGACCTGCC CCCTCAGCCT CTCAGCTTTG CAATGTCCCT 
TGCTCGATGG ACTGCATAGT ATCTTCCTGG TCAACCTGGG GCCCATGTGT TTTTGAGAAC 
TGCCATGATC CTCAGGGGAA GAAAGGCTTT AGAATGAGAC AGCGCCATGT ACTCATGGAG 
TCTACAGGGC CCATGGGGCG CTGCCCTCAT CTGGCAGAAT CTGTACCTTG TGAGGATCCA 
ATGTGTCATC GCTGGCTTGC CTCGGAAGGG ATCTGTATTG CTGATCATGG GAAATGTGGC 
CTGGGACATC GCATTCTGAA GGCTGTCTGT CAGAATGAGC GAGGAGAAGA AGTATCAGGG 
GGTCTCTGTC CAGTGCCTCC TCCTCCTGAA CGAATGGCTT GTGAAATTCC TTGCCGAATG 
GACTGTGTAG TGAGTGAGTG GACAGTGTGG TCATCCTGCT CTCAGTCCTG TTCAAATAAA 
AACTCAGATG GGAAACAAAC CAGGTCACGT TCTATCCTCG CTTTGGCTGG AGAAGGTGGA 
AAGACCTGCC CTAGTAGTCA GGAACTCCAA GAGTACCGCC TATGCAATGA TCATTCCTGT 
ACACAGCTCT ACTGGGAGAC ATCTGCTTGG GGTTCCTGCT CTGAAAATAC TCTTGTGACT 
GCTCTCAATG TGACCATTGG CTGGAATGGA GAAGCAACAT GTGGGGTGGG CATTCAGACA 
CGAAAGGTCT TCTGTATCAA GAGTCATGTG GGACAAGTAA TGACCAAAAG ATGTCCAGAG 
TCTACACGGC CAGAAACTGT AAGACCTTGT TTCCTCCCAT GCAAGAAAGA CTGCCTTGTG 
ACTGCTTTCA GTGAGTGGAC ACCATGCCCA CGGCCCTGCC AGCCAGGAAA TACTACAATA 
AAACAATCTC GATACAGGAT TATCATCCAG GAAGCAGCCA ATGGGGGTCA AGAGTGCCCA 
GACACCTTGT TTGAAGAAAG AGAGTGTGAA GATATCTCAC TGTGTCCTTC ATACCGGTGG 
AAGCCGCAGA AATGGAGCTC TTGTATCCTG GTTCCAGAGT CTATCAGGCA GGGGAGAACA 
GGCACCAGTG AGGCATGTGG GAAGGGGCTG CAGACAAGAG CTGTCTCATG CATCTCTGAT 
GATAACCAGT CTGCAGAAAT GACAGAGTGC CTCAAGCAGA TGAATGGCAT GCCTCCCCTT 
GTGCAGGAAT GCACCATTCC CTGTCGGGAC GACTGCACCT TCACCCCTTG GTCTAAGTTC 
ACTCCCTGCT CCAAGAACTG TGAAGCAACT CAGATCAGGA GGCGGCAGCT AACAGGGAAA 
AGCAGGAAGA AAGAGAAGTG CCAGGATGCG TCCCTGTACC CTCTGGTGGA AATGGAACCA 
TGTCCCTGTG ATACGTTTAT GTCTCATCCT TATGGAAACT GGTCAGCTTG CATTCTACCA 
GAAGGCAAAA GGGATGCTCA GCAAGGAGGA TTGTGGGTGC AAGGAGATGA CAAAGAATGT 
GGAGAAGGCG TGCGCTTTCG AGCAATAGCA TGCTCCAATA TAAATGGAAG ACCGGTTGAC 
CCCTCATTCT GCAACAGCTC TGGTTACATT CAGGAAGCAT GTGTCATCCC CTGTCCGTTT 
GATTGCAAGC TAAGTGACTG GTCCAGCTGG GGTTCTTGCA GCTCATCTTG TGGGATTGGA 
GTGAGAATTC GATCAAAATG GCTAAAAGAA AAACCTTACA GTGGAGGTCG GCCATGTCCC 
AAACTGGATC TCAAGAATCA GGCTCAGGTT CATGAGGCAG TCCCATGTTA CAGTGAGTGT 
GATCAATATT CCTGGGTCGT AGAACACTGG TCTCCATGCA AGATCAATAA TGAGCTGAGA 
TCCCCACGCT GTGGAAGAGG AACACAGTCT AGAAGAATCA GGTGTGTGAG TACTGCTGAC 
AGAGAAGGAG GGGCAGTGAA CAGGAGCCTG TGCAACCAGG ATGATGCTCC CCAAGAAACC 
CAGGCCTGTT CTCTTCTGTG TCCCAGTGAG TGTGTCATGT CCGAGTGGGG AACATGGAGC 
AGATGCCCAC AGTCATGTGA CCCCCATGCA ATGCAGAGAA GAACTCGGCA TCTGCTGAGG 
CCCTCACTGA ACTCAAGAAC TTGTGGGGAA GACTCGCAGG TGCGGCCCTG CCTCCTCAAT 
GAAAACTGTT TCCAGTTCCA GTACAATCTG ACAGAGTGGA GCACATGCCA GCTGAGTGAA 
AATGTTTCCT GTGGCCAGGG TGTGAGGACC CGCCTGCTGA GCTGTGTGCG CAGTGATGGC 
AAGTCTGTCA GCATGGACCA CTGCGAGCAG CGTAATCTGG AAAAACCCCA GAGGATGAGT 
ATTCCCTGTC TGGTGGAATG TGTGGTCAAC TGTCAGCTCT CAGGATGGAC AACATGGACA 
GAGTGTTCAC AAACCTGTGG CCAAGGAGGT CGCATGAGCC GGACTCGATT TATCATTATG 
CCAACCCAAG GAGAAGGTCG ACAATGCCCC ACAGAACTCA CCCAGCAGAA ACCCTGCCCA 
GTGACACCCT GCTATAGTTG GGTCCTTGGC AACTGGTCTG CATGTAAATT GGAGGGCGGA 
GATTGTGGAG AAGGAGTCCA GGTGCGCAGC TTCTCCTGTG TAGTCCACAA TGGCTCAATA 
TCCCACACGG CTGTCCCTGT GGAGGAGGCC CTGTGTGGAG AAGTACCCTT CCAAGAAGGA 
ATCCTGAAGC AGCTGTGCTC TGTGCCTTGC CCAGGAGACT GCCACATTAC CCCATGGTCT 
GAGTGGAGCA AGTGTGAGCT AACCTGCATT GACGGAAGAA GCTTCGAGAC CACAGGCCGC 
CAATCTAGGT CAAGGACATT CATAATCCAG TCTTTTGAGA ATCAAGATAG CTGCCCCCAA 
CAGGTTCTAG AAACACGACC ATGTACAGGT GGCAAATGTT ATCACTACAT ATGGAAAGCA 
AGTCTTTGGA ACAATAATGA GCGGACTGTG TGGTGCCAGC GCTCAGATGG CCTTAATGTC 
ACAGGTGGCT GCTCCCCTCA GGCACGCCCT GCTGCTATTC GGCAATGCAT TCCAGCATGC 
AAAAAACCTT TCTCCTACTG CACACAGGGA GGAGTCTGTG GCTGTGAGAA GGGATACACA 
GAGATAATGA GATCAAGTGG CTTCCTGGAT TACTGCATGA AGGTGCCTGG CTCGGAGGAT 
AAAAAAGCTG ACGTGAAAAA CCTCTCTGGG AAAAATAGAC CTGTGAATTC CAAAATACAT 
GATATTTTTA AAGGATGGTC TCTCCAACCT CTGGACCCAG ATGGCCGAGT AAAAATGTGG 
GTTTATGGCG TTTCCGGTGG CAGTTTTCTT ATCATGATCT TCCTAGTATT TACTTCCTAT 
CTTGTTTGCA AGAAGCCAAA ACCACATCAA AGCACACCTC GCCATCAGAA GCCACTGACC 
TTGGCCTACG ATGGAGACTT GGACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

O-fucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) and maintains epiblast pluripotency during mouse gastrulation.
Developmental biology346(1)25-38(2010 Oct)
Du J,Takeuchi H,Leonhard-Melief C,Shroyer KR,Dlugosz M,Haltiwanger RS,Holdener BC

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Thsd7b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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