Vamp7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vamp7
Entrez Gene ID	20955
Full Name	vesicle-associated membrane protein 7
Synonyms	Sybl1,TI-VAMP,VAMP-7
General protein information	Preferred Names vesicle-associated membrane protein 7 Names vesicle-associated membrane protein 7 synaptobrevin like 1 tetanus neurotoxin-insensitive vesicle-associated membrane protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X

mRNA and Protein(s)

mRNA	Protein	Name
XM_011249809.2	XP_011248111.1	vesicle-associated membrane protein 7 isoform X1
NM_001359151.1	NP_001346080.1	vesicle-associated membrane protein 7 isoform 3
NM_001302138.1	NP_001289067.1	vesicle-associated membrane protein 7 isoform 2
XM_011249809.3	XP_011248111.1	vesicle-associated membrane protein 7 isoform X1
XM_006535856.3	XP_006535919.1	vesicle-associated membrane protein 7 isoform X2
NM_011515.5	NP_035645.1	vesicle-associated membrane protein 7 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	VAMP7	NP_005629.1
Pan troglodytes (chimpanzee)	VAMP7	XP_003317850.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000700	XP_003437068.1
Mus musculus (house mouse)	Vamp7	NP_035645.1
Rattus norvegicus (Norway rat)	Vamp7	NP_445983.1
Gallus gallus (chicken)	VAMP7	NP_001026292.1
Drosophila melanogaster (fruit fly)	CG1599	NP_610524.1
Oryza sativa (rice)	Os10g0154000	NP_001064184.1
Canis lupus familiaris (dog)	VAMP7	XP_855489.3
Bos taurus (cattle)	VAMP7	NP_001069770.1
Danio rerio (zebrafish)	sybl1	NP_956560.1
Arabidopsis thaliana (thale cress)	VAMP714	NP_197628.1
Xenopus tropicalis (tropical clawed frog)	vamp7	NP_001135622.1

Gene Ontology
Bibliography

Related articles in PubMed

Spastin regulates VAMP7-containing vesicles trafficking in cortical neurons.
Plaud C, Joshi V, Marinello M, Pastr? D, Galli T, Curmi PA, Burgo A
Biochimica et biophysica acta1863(6)1666-1677(2017 Jun)

Structure and function of longin SNAREs.
Daste F, Galli T, Tareste D
Journal of cell science128(23)4263-72(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

VAMP7 mediates fusion of BLOC-1-dependent transport carriers with melanosomes, illuminate SNARE recycling from melanosomes as a critical BLOC-3-dependent step.
Title: BLOC-1 and BLOC-3 regulate VAMP7 cycling to and from melanosomes via distinct tubular transport carriers.

VAMP7-mediated release of interleukin-12 at the immune synapse is a mechanism to transmit innate signals to T cells.
Title: The SNARE VAMP7 Regulates Exocytic Trafficking of Interleukin-12 in Dendritic Cells.

VAMP7 mediates plasma membrane fusion of vesicles containing TRPM8. VAMP7-deficient mice exhibit reduced functional expression of TRPM8 in sensory neurons and concomitant deficits in cold avoidance and icilin-induced cold hypersensitivity.
Title: VAMP7 regulates constitutive membrane incorporation of the cold-activated channel TRPM8.

VAMP-7(-/-) platelets demonstrated a partial defect in dense granule exocytosis and impaired aggregation. Consistent with a role in cytoskeletal remodeling, spreading on matrices was decreased in VAMP-7(-/-) platelets compared to wild-type controls.
Title: VAMP-7 links granule exocytosis to actin reorganization during platelet activation.

syntaxin 1 and vesicle-associated membrane protein 1 are more suitable targets to abolish functional soluble N-ethylmaleimide-sensitive factor attachment protein receptor complexes
Title: Selective cleavage of SNAREs in sensory neurons unveils protein complexes mediating peptide exocytosis triggered by different stimuli.

The following Vamp7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vamp7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu57232	NM_001359151.1 Latest version!	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu57232	XM_006535856.3 Latest version!	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu68826	NM_001302138.1 Latest version!	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu19192	NM_011515.5 Latest version!	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu68826	XM_011249809.3 Latest version!	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu68826	XM_011249809.2	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vamp7 ( NM_001359151.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346080.1 Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu57232
Clone ID Related Accession (Same CDS sequence)	NM_001359151.1 , XM_006535856.3
Accession Version	NM_001359151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	vesicle-associated membrane protein 7 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CAAA01165726.1 and BP768744.1. On Dec 22, 2017 this sequence version replaced XM_006535856.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.119182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATAGCG AGTTTTCAAG TGTTTTGGCT GCACAACTGA AGCATCACTC TGAGAATAAG 
AGCCTAGACA AAGTGATGGA GACTCAAGCA CAAGTGGATG AACTGAAAGG AATAATGGTC 
AGAAACATAG ATTTAGTTGC TCAACGTGGA GAAAGGTTGG AATTGCTGAT AGATAAAACA 
GAAAACCTTG TGGATTCATC TGTCACTTTC AAAACTACCA GCAGAAATCT TGCTCGAGCC 
ATGTGTATGA AGAATATCAA GCTCACTATC ATCATCATCA TTGTATCAAT TGTGTTCATC 
TATATCATTG TGTCACTTCT CTGTGGTGGG TTTACATGGC CAAACTGTGT GAAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346080.1
CDS	442..801
Translation	MNSEFSSVLAAQLKHHSENKSLDKVMETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLARAMCMKNIKLTIIIIIVSIVFIYIIVSLLCGGFTWPNCVKK

Target ORF information:

RefSeq Version	NM_001359151.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359151.1

ORF Insert Sequence:

ATGAATAGCG AGTTTTCAAG TGTTTTGGCT GCACAACTGA AGCATCACTC TGAGAATAAG 
AGCCTAGACA AAGTGATGGA GACTCAAGCA CAAGTGGATG AACTGAAAGG AATAATGGTC 
AGAAACATAG ATTTAGTTGC TCAACGTGGA GAAAGGTTGG AATTGCTGAT AGATAAAACA 
GAAAACCTTG TGGATTCATC TGTCACTTTC AAAACTACCA GCAGAAATCT TGCTCGAGCC 
ATGTGTATGA AGAATATCAA GCTCACTATC ATCATCATCA TTGTATCAAT TGTGTTCATC 
TATATCATTG TGTCACTTCT CTGTGGTGGG TTTACATGGC CAAACTGTGT GAAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vamp7 ( XM_006535856.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535919.1 Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu57232
Clone ID Related Accession (Same CDS sequence)	NM_001359151.1 , XM_006535856.3
Accession Version	XM_006535856.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	vesicle-associated membrane protein 7 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_165789.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAGCG AGTTTTCAAG TGTTTTGGCT GCACAACTGA AGCATCACTC TGAGAATAAG 
AGCCTAGACA AAGTGATGGA GACTCAAGCA CAAGTGGATG AACTGAAAGG AATAATGGTC 
AGAAACATAG ATTTAGTTGC TCAACGTGGA GAAAGGTTGG AATTGCTGAT AGATAAAACA 
GAAAACCTTG TGGATTCATC TGTCACTTTC AAAACTACCA GCAGAAATCT TGCTCGAGCC 
ATGTGTATGA AGAATATCAA GCTCACTATC ATCATCATCA TTGTATCAAT TGTGTTCATC 
TATATCATTG TGTCACTTCT CTGTGGTGGG TTTACATGGC CAAACTGTGT GAAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535919.1
CDS	431..790
Translation	MNSEFSSVLAAQLKHHSENKSLDKVMETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLARAMCMKNIKLTIIIIIVSIVFIYIIVSLLCGGFTWPNCVKK

Target ORF information:

RefSeq Version	XM_006535856.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535856.3

ORF Insert Sequence:

ATGAATAGCG AGTTTTCAAG TGTTTTGGCT GCACAACTGA AGCATCACTC TGAGAATAAG 
AGCCTAGACA AAGTGATGGA GACTCAAGCA CAAGTGGATG AACTGAAAGG AATAATGGTC 
AGAAACATAG ATTTAGTTGC TCAACGTGGA GAAAGGTTGG AATTGCTGAT AGATAAAACA 
GAAAACCTTG TGGATTCATC TGTCACTTTC AAAACTACCA GCAGAAATCT TGCTCGAGCC 
ATGTGTATGA AGAATATCAA GCTCACTATC ATCATCATCA TTGTATCAAT TGTGTTCATC 
TATATCATTG TGTCACTTCT CTGTGGTGGG TTTACATGGC CAAACTGTGT GAAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vamp7 ( NM_001302138.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289067.1 Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu68826
Clone ID Related Accession (Same CDS sequence)	XM_011249809.2 , NM_001302138.1 , XM_011249809.3
Accession Version	NM_001302138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	vesicle-associated membrane protein 7 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK165299.1, AK002825.1 and BP768744.1. On Oct 3, 2014 this sequence version replaced XM_006535855.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB232359.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTTGGTG TGGAGGAAAC TTCCTGGAGT TATTTGTTTC ATTACATCTG CCAAGACAGG 
ATTGTGTATC TTTGTATCAC TGATGATGAT TTTGAGCGTT CTCGAGCGTT CAGTTTTTTG 
AATGAAGTAA AGAAGAGATT CCAGACAACT TACGGTTCAA GAGCACAAAC TGCACTTCCT 
TATGCTATGA ATAGCGAGTT TTCAAGTGTT TTGGCTGCAC AACTGAAGCA TCACTCTGAG 
AATAAGAGCC TAGACAAAGT GATGGAGACT CAAGCACAAG TGGATGAACT GAAAGGAATA 
ATGGTCAGAA ACATAGATTT AGTTGCTCAA CGTGGAGAAA GGTTGGAATT GCTGATAGAT 
AAAACAGAAA ACCTTGTGGA TTCATCTGTC ACTTTCAAAA CTACCAGCAG AAATCTTGCT 
CGAGCCATGT GTATGAAGAA TATCAAGCTC ACTATCATCA TCATCATTGT ATCAATTGTG 
TTCATCTATA TCATTGTGTC ACTTCTCTGT GGTGGGTTTA CATGGCCAAA CTGTGTGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289067.1
CDS	229..774
Translation	MLGVEETSWSYLFHYICQDRIVYLCITDDDFERSRAFSFLNEVKKRFQTTYGSRAQTALPYAMNSEFSSVLAAQLKHHSENKSLDKVMETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLARAMCMKNIKLTIIIIIVSIVFIYIIVSLLCGGFTWPNCVKK

Target ORF information:

RefSeq Version	NM_001302138.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302138.1

ORF Insert Sequence:

ATGCTTGGTG TGGAGGAAAC TTCCTGGAGT TATTTGTTTC ATTACATCTG CCAAGACAGG 
ATTGTGTATC TTTGTATCAC TGATGATGAT TTTGAGCGTT CTCGAGCGTT CAGTTTTTTG 
AATGAAGTAA AGAAGAGATT CCAGACAACT TACGGTTCAA GAGCACAAAC TGCACTTCCT 
TATGCTATGA ATAGCGAGTT TTCAAGTGTT TTGGCTGCAC AACTGAAGCA TCACTCTGAG 
AATAAGAGCC TAGACAAAGT GATGGAGACT CAAGCACAAG TGGATGAACT GAAAGGAATA 
ATGGTCAGAA ACATAGATTT AGTTGCTCAA CGTGGAGAAA GGTTGGAATT GCTGATAGAT 
AAAACAGAAA ACCTTGTGGA TTCATCTGTC ACTTTCAAAA CTACCAGCAG AAATCTTGCT 
CGAGCCATGT GTATGAAGAA TATCAAGCTC ACTATCATCA TCATCATTGT ATCAATTGTG 
TTCATCTATA TCATTGTGTC ACTTCTCTGT GGTGGGTTTA CATGGCCAAA CTGTGTGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vamp7 ( XM_011249809.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248111.1 Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68826
Clone ID Related Accession (Same CDS sequence)	XM_011249809.2 , NM_001302138.1 , XM_011249809.3
Accession Version	XM_011249809.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	vesicle-associated membrane protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_165789.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG TGGAGGAAAC TTCCTGGAGT TATTTGTTTC ATTACATCTG CCAAGACAGG 
ATTGTGTATC TTTGTATCAC TGATGATGAT TTTGAGCGTT CTCGAGCGTT CAGTTTTTTG 
AATGAAGTAA AGAAGAGATT CCAGACAACT TACGGTTCAA GAGCACAAAC TGCACTTCCT 
TATGCTATGA ATAGCGAGTT TTCAAGTGTT TTGGCTGCAC AACTGAAGCA TCACTCTGAG 
AATAAGAGCC TAGACAAAGT GATGGAGACT CAAGCACAAG TGGATGAACT GAAAGGAATA 
ATGGTCAGAA ACATAGATTT AGTTGCTCAA CGTGGAGAAA GGTTGGAATT GCTGATAGAT 
AAAACAGAAA ACCTTGTGGA TTCATCTGTC ACTTTCAAAA CTACCAGCAG AAATCTTGCT 
CGAGCCATGT GTATGAAGAA TATCAAGCTC ACTATCATCA TCATCATTGT ATCAATTGTG 
TTCATCTATA TCATTGTGTC ACTTCTCTGT GGTGGGTTTA CATGGCCAAA CTGTGTGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248111.1
CDS	180..725
Translation	MLGVEETSWSYLFHYICQDRIVYLCITDDDFERSRAFSFLNEVKKRFQTTYGSRAQTALPYAMNSEFSSVLAAQLKHHSENKSLDKVMETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLARAMCMKNIKLTIIIIIVSIVFIYIIVSLLCGGFTWPNCVKK

Target ORF information:

RefSeq Version	XM_011249809.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249809.2

ORF Insert Sequence:

ATGCTTGGTG TGGAGGAAAC TTCCTGGAGT TATTTGTTTC ATTACATCTG CCAAGACAGG 
ATTGTGTATC TTTGTATCAC TGATGATGAT TTTGAGCGTT CTCGAGCGTT CAGTTTTTTG 
AATGAAGTAA AGAAGAGATT CCAGACAACT TACGGTTCAA GAGCACAAAC TGCACTTCCT 
TATGCTATGA ATAGCGAGTT TTCAAGTGTT TTGGCTGCAC AACTGAAGCA TCACTCTGAG 
AATAAGAGCC TAGACAAAGT GATGGAGACT CAAGCACAAG TGGATGAACT GAAAGGAATA 
ATGGTCAGAA ACATAGATTT AGTTGCTCAA CGTGGAGAAA GGTTGGAATT GCTGATAGAT 
AAAACAGAAA ACCTTGTGGA TTCATCTGTC ACTTTCAAAA CTACCAGCAG AAATCTTGCT 
CGAGCCATGT GTATGAAGAA TATCAAGCTC ACTATCATCA TCATCATTGT ATCAATTGTG 
TTCATCTATA TCATTGTGTC ACTTCTCTGT GGTGGGTTTA CATGGCCAAA CTGTGTGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vamp7 ( NM_011515.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035645.1 Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19192
Clone ID Related Accession (Same CDS sequence)	NM_011515.5
Accession Version	NM_011515.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	vesicle-associated membrane protein 7 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK165299.1, AK002825.1 and BP768744.1. On Oct 3, 2014 this sequence version replaced NM_011515.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.79723.1, AK002825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCCATTC TTTTTGCTGT TGTTGCCAGG GGAACCACTA TCCTTGCCAA ACATGCTTGG 
TGTGGAGGAA ACTTCCTGGA GGTGACAGAG CAGATTCTGG CTAAGATACC TTCTGAAAAT 
AATAAACTAA CTTACTCACA TGGCAATTAT TTGTTTCATT ACATCTGCCA AGACAGGATT 
GTGTATCTTT GTATCACTGA TGATGATTTT GAGCGTTCTC GAGCGTTCAG TTTTTTGAAT 
GAAGTAAAGA AGAGATTCCA GACAACTTAC GGTTCAAGAG CACAAACTGC ACTTCCTTAT 
GCTATGAATA GCGAGTTTTC AAGTGTTTTG GCTGCACAAC TGAAGCATCA CTCTGAGAAT 
AAGAGCCTAG ACAAAGTGAT GGAGACTCAA GCACAAGTGG ATGAACTGAA AGGAATAATG 
GTCAGAAACA TAGATTTAGT TGCTCAACGT GGAGAAAGGT TGGAATTGCT GATAGATAAA 
ACAGAAAACC TTGTGGATTC ATCTGTCACT TTCAAAACTA CCAGCAGAAA TCTTGCTCGA 
GCCATGTGTA TGAAGAATAT CAAGCTCACT ATCATCATCA TCATTGTATC AATTGTGTTC 
ATCTATATCA TTGTGTCACT TCTCTGTGGT GGGTTTACAT GGCCAAACTG TGTGAAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035645.1
CDS	177..839
Translation	MAILFAVVARGTTILAKHAWCGGNFLEVTEQILAKIPSENNKLTYSHGNYLFHYICQDRIVYLCITDDDFERSRAFSFLNEVKKRFQTTYGSRAQTALPYAMNSEFSSVLAAQLKHHSENKSLDKVMETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLARAMCMKNIKLTIIIIIVSIVFIYIIVSLLCGGFTWPNCVKK

Target ORF information:

RefSeq Version	NM_011515.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011515.5

ORF Insert Sequence:

ATGGCCATTC TTTTTGCTGT TGTTGCCAGG GGAACCACTA TCCTTGCCAA ACATGCTTGG 
TGTGGAGGAA ACTTCCTGGA GGTGACAGAG CAGATTCTGG CTAAGATACC TTCTGAAAAT 
AATAAACTAA CTTACTCACA TGGCAATTAT TTGTTTCATT ACATCTGCCA AGACAGGATT 
GTGTATCTTT GTATCACTGA TGATGATTTT GAGCGTTCTC GAGCGTTCAG TTTTTTGAAT 
GAAGTAAAGA AGAGATTCCA GACAACTTAC GGTTCAAGAG CACAAACTGC ACTTCCTTAT 
GCTATGAATA GCGAGTTTTC AAGTGTTTTG GCTGCACAAC TGAAGCATCA CTCTGAGAAT 
AAGAGCCTAG ACAAAGTGAT GGAGACTCAA GCACAAGTGG ATGAACTGAA AGGAATAATG 
GTCAGAAACA TAGATTTAGT TGCTCAACGT GGAGAAAGGT TGGAATTGCT GATAGATAAA 
ACAGAAAACC TTGTGGATTC ATCTGTCACT TTCAAAACTA CCAGCAGAAA TCTTGCTCGA 
GCCATGTGTA TGAAGAATAT CAAGCTCACT ATCATCATCA TCATTGTATC AATTGTGTTC 
ATCTATATCA TTGTGTCACT TCTCTGTGGT GGGTTTACAT GGCCAAACTG TGTGAAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vamp7 ( XM_011249809.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248111.1 Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant X1, mRNA.

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CloneID	OMu68826
Clone ID Related Accession (Same CDS sequence)	XM_011249809.2 , NM_001302138.1 , XM_011249809.3
Accession Version	XM_011249809.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	vesicle-associated membrane protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_165789.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG TGGAGGAAAC TTCCTGGAGT TATTTGTTTC ATTACATCTG CCAAGACAGG 
ATTGTGTATC TTTGTATCAC TGATGATGAT TTTGAGCGTT CTCGAGCGTT CAGTTTTTTG 
AATGAAGTAA AGAAGAGATT CCAGACAACT TACGGTTCAA GAGCACAAAC TGCACTTCCT 
TATGCTATGA ATAGCGAGTT TTCAAGTGTT TTGGCTGCAC AACTGAAGCA TCACTCTGAG 
AATAAGAGCC TAGACAAAGT GATGGAGACT CAAGCACAAG TGGATGAACT GAAAGGAATA 
ATGGTCAGAA ACATAGATTT AGTTGCTCAA CGTGGAGAAA GGTTGGAATT GCTGATAGAT 
AAAACAGAAA ACCTTGTGGA TTCATCTGTC ACTTTCAAAA CTACCAGCAG AAATCTTGCT 
CGAGCCATGT GTATGAAGAA TATCAAGCTC ACTATCATCA TCATCATTGT ATCAATTGTG 
TTCATCTATA TCATTGTGTC ACTTCTCTGT GGTGGGTTTA CATGGCCAAA CTGTGTGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248111.1
CDS	178..723
Translation	MLGVEETSWSYLFHYICQDRIVYLCITDDDFERSRAFSFLNEVKKRFQTTYGSRAQTALPYAMNSEFSSVLAAQLKHHSENKSLDKVMETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLARAMCMKNIKLTIIIIIVSIVFIYIIVSLLCGGFTWPNCVKK

Target ORF information:

RefSeq Version	XM_011249809.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus vesicle-associated membrane protein 7 (Vamp7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249809.3

ORF Insert Sequence:

ATGCTTGGTG TGGAGGAAAC TTCCTGGAGT TATTTGTTTC ATTACATCTG CCAAGACAGG 
ATTGTGTATC TTTGTATCAC TGATGATGAT TTTGAGCGTT CTCGAGCGTT CAGTTTTTTG 
AATGAAGTAA AGAAGAGATT CCAGACAACT TACGGTTCAA GAGCACAAAC TGCACTTCCT 
TATGCTATGA ATAGCGAGTT TTCAAGTGTT TTGGCTGCAC AACTGAAGCA TCACTCTGAG 
AATAAGAGCC TAGACAAAGT GATGGAGACT CAAGCACAAG TGGATGAACT GAAAGGAATA 
ATGGTCAGAA ACATAGATTT AGTTGCTCAA CGTGGAGAAA GGTTGGAATT GCTGATAGAT 
AAAACAGAAA ACCTTGTGGA TTCATCTGTC ACTTTCAAAA CTACCAGCAG AAATCTTGCT 
CGAGCCATGT GTATGAAGAA TATCAAGCTC ACTATCATCA TCATCATTGT ATCAATTGTG 
TTCATCTATA TCATTGTGTC ACTTCTCTGT GGTGGGTTTA CATGGCCAAA CTGTGTGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spastin regulates VAMP7-containing vesicles trafficking in cortical neurons.
Biochimica et biophysica acta1863(6)1666-1677(2017 Jun)
Plaud C,Joshi V,Marinello M,Pastr? D,Galli T,Curmi PA,Burgo A

Structure and function of longin SNAREs.
Journal of cell science128(23)4263-72(2015 Dec)
Daste F,Galli T,Tareste D

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Vamp7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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