Hsh2d cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsh2d
Entrez Gene ID	209488
Full Name	hematopoietic SH2 domain containing
Synonyms	ALX,Hsh2
General protein information	Preferred Names hematopoietic SH2 domain containing Names hematopoietic SH2 domain-containing protein adaptor in lymphocytes of unknown function X adaptor protein ALx2 hematopoietic SH2 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_197944.1	NP_922935.1	hematopoietic SH2 domain-containing protein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hsh2d	NP_922935.1
Homo sapiens (human)	HSH2D	NP_116244.1
Pan troglodytes (chimpanzee)	HSH2D	NP_001229302.1
Canis lupus familiaris (dog)	HSH2D	XP_005632809.1
Bos taurus (cattle)	HSH2D	XP_003586295.2
Rattus norvegicus (Norway rat)	Hsh2d	NP_001258257.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential expression of the adaptor protein HSH2 controls the quantitative and qualitative nature of the humoral response.
King RG, Herrin BR, Justement LB
Journal of immunology (Baltimore, Md. : 1950)187(7)3565-77(2011 Oct)

The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes.
Perchonock CE, Pajerowski AG, Nguyen C, Shapiro MJ, Shapiro VS
Journal of immunology (Baltimore, Md. : 1950)179(3)1768-75(2007 Aug)

Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX.
Perchonock CE, Fernando MC, Quinn WJ 3rd, Nguyen CT, Sun J, Shapiro MJ, Shapiro VS
Molecular and cellular biology26(16)6005-15(2006 Aug)

Expression of the adaptor protein hematopoietic Src homology 2 is up-regulated in response to stimuli that promote survival and differentiation of B cells.
Herrin BR, Justement LB
Journal of immunology (Baltimore, Md. : 1950)176(7)4163-72(2006 Apr)

The adaptor protein HSH2 attenuates apoptosis in response to ligation of the B cell antigen receptor complex on the B lymphoma cell line, WEHI-231.
Herrin BR, Groeger AL, Justement LB
The Journal of biological chemistry280(5)3507-15(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data demonstrate that changes in HSH2 expression have profound effects on the humoral immune response.
Title: Differential expression of the adaptor protein HSH2 controls the quantitative and qualitative nature of the humoral response.

Although adaptor in lymphocytes of unknown function X (ALX) is coexpressed with related adaptor Rlk/Itk-binding protein (RIBP) on both T- and B-lymphocytes throughout cell development, no redundancy or synergy is found.
Title: The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes.

These results suggest that ALX, in association with LAX, negatively regulates T-cell activation through inhibition of p38.
Title: Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX.

HSH2 expression is coordinately up-regulated with known antiapoptotic molecules and directly correlates with B cell survival
Title: Expression of the adaptor protein hematopoietic Src homology 2 is up-regulated in response to stimuli that promote survival and differentiation of B cells.

endogenous HSH2 expression correlates directly with survival of WEHI-231 cells, which supports the hypothesis that HSH2 modulates the apoptotic response through its ability to directly or indirectly promote mitochondrial stability
Title: The adaptor protein HSH2 attenuates apoptosis in response to ligation of the B cell antigen receptor complex on the B lymphoma cell line, WEHI-231.

The following Hsh2d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsh2d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08734	NM_197944.1 Latest version!	Mus musculus hematopoietic SH2 domain containing (Hsh2d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsh2d ( NM_197944.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_922935.1 Mus musculus hematopoietic SH2 domain containing (Hsh2d), mRNA.

Price & Availability↑

CloneID	OMu08734
Clone ID Related Accession (Same CDS sequence)	NM_197944.1
Accession Version	NM_197944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	hematopoietic SH2 domain-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY319653.1. ##Evidence-Data-START## Transcript exon combination :: AY319653.1, AK157000.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAAG CCAGGAGGCT GCCCCCACCA CTACCTCCTC GGCTGGACTG GTTTGTGCAC 
ACCCAGGCGG ACCTGCTAGC CCAATCCGGG ATCCCCGAGT GGTTCCATGG CACCATCTCC 
CGAGAGGCTG CTGAGAACAT GTTGGAGTCA CAACCCCTGG GGACTTTTCT TATCCGAGTC 
AGCCACAGCC ATGTGGGCTA CACCCTTTCC TACAAAGCCC AGACTTGCTG CCGTCACTTC 
ATGGTGAAAC TTTCAGAAGA TGGGACTTGT GCCTTCGCTG GGGACCACAT GACCCACGCC 
TCTCTGCATG CCCTGGTCAC CTTCCACCAA CAAAAGCCTA TTCGGCCATT CGGGGAGCTG 
TTGACCCAGG CCTGCGGGCA GGAGGATCCA GCAAACGTGG ACTATGAGGA TCTCTTCCTA 
TACTCAAATG CCCTGGTTCA GGATGCTGAA AGCCAAATTC TGAGAACGGA AGTTCAAAGG 
TCTTCCTGCC CACCTGAGGA GGCTTCTGAA AGGAAGCCAT CCACAACAAC TAAAGGAGAA 
TTCGCATCAG CTTCCTGCTC CCCAAAAGCC CTTTTCGAGG ATTCTGGACA GAAACTGTGG 
AAGAACCTGA GGTCCCTACC TCAGACTAGC CAGAGGGTGA AACAGCGACT CACTTCACAC 
CTGTTGGCCA TGAACCTGCT GGGGGATGCC AGGCAAGTGG CACAGCAGCA CCACAGCCCT 
GTGACTCGAG CATTCAGTTG GGACAGTACT AGCCACTCCG AGGACTCCTG TGCAGCCACC 
ACGTCTCTTC AAAACCCTGC AGAACCCCAG GCCCTGAGAG GCAGAGAAGC CACCTTCAGG 
GACTCCAGGC CTGCCAGCTG GAGGAAGGCA TTCTCAGGGG TCAAAGCCTG GCGTGGAAAG 
GTGGTAAGGG CCCTGTCAGC CCAGGAGCCT GTGGACTTTC CAGAAGCCCA GGGCTGGTTG 
CCTGAGGAAT ACCTTCCACC ACCACCTTTC GCCCCTGGGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_922935.1
CDS	43..1047
Translation	MAEARRLPPPLPPRLDWFVHTQADLLAQSGIPEWFHGTISREAAENMLESQPLGTFLIRVSHSHVGYTLSYKAQTCCRHFMVKLSEDGTCAFAGDHMTHASLHALVTFHQQKPIRPFGELLTQACGQEDPANVDYEDLFLYSNALVQDAESQILRTEVQRSSCPPEEASERKPSTTTKGEFASASCSPKALFEDSGQKLWKNLRSLPQTSQRVKQRLTSHLLAMNLLGDARQVAQQHHSPVTRAFSWDSTSHSEDSCAATTSLQNPAEPQALRGREATFRDSRPASWRKAFSGVKAWRGKVVRALSAQEPVDFPEAQGWLPEEYLPPPPFAPGY

Target ORF information:

RefSeq Version	NM_197944.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hematopoietic SH2 domain containing (Hsh2d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_197944.1

ORF Insert Sequence:

ATGGCAGAAG CCAGGAGGCT GCCCCCACCA CTACCTCCTC GGCTGGACTG GTTTGTGCAC 
ACCCAGGCGG ACCTGCTAGC CCAATCCGGG ATCCCCGAGT GGTTCCATGG CACCATCTCC 
CGAGAGGCTG CTGAGAACAT GTTGGAGTCA CAACCCCTGG GGACTTTTCT TATCCGAGTC 
AGCCACAGCC ATGTGGGCTA CACCCTTTCC TACAAAGCCC AGACTTGCTG CCGTCACTTC 
ATGGTGAAAC TTTCAGAAGA TGGGACTTGT GCCTTCGCTG GGGACCACAT GACCCACGCC 
TCTCTGCATG CCCTGGTCAC CTTCCACCAA CAAAAGCCTA TTCGGCCATT CGGGGAGCTG 
TTGACCCAGG CCTGCGGGCA GGAGGATCCA GCAAACGTGG ACTATGAGGA TCTCTTCCTA 
TACTCAAATG CCCTGGTTCA GGATGCTGAA AGCCAAATTC TGAGAACGGA AGTTCAAAGG 
TCTTCCTGCC CACCTGAGGA GGCTTCTGAA AGGAAGCCAT CCACAACAAC TAAAGGAGAA 
TTCGCATCAG CTTCCTGCTC CCCAAAAGCC CTTTTCGAGG ATTCTGGACA GAAACTGTGG 
AAGAACCTGA GGTCCCTACC TCAGACTAGC CAGAGGGTGA AACAGCGACT CACTTCACAC 
CTGTTGGCCA TGAACCTGCT GGGGGATGCC AGGCAAGTGG CACAGCAGCA CCACAGCCCT 
GTGACTCGAG CATTCAGTTG GGACAGTACT AGCCACTCCG AGGACTCCTG TGCAGCCACC 
ACGTCTCTTC AAAACCCTGC AGAACCCCAG GCCCTGAGAG GCAGAGAAGC CACCTTCAGG 
GACTCCAGGC CTGCCAGCTG GAGGAAGGCA TTCTCAGGGG TCAAAGCCTG GCGTGGAAAG 
GTGGTAAGGG CCCTGTCAGC CCAGGAGCCT GTGGACTTTC CAGAAGCCCA GGGCTGGTTG 
CCTGAGGAAT ACCTTCCACC ACCACCTTTC GCCCCTGGGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential expression of the adaptor protein HSH2 controls the quantitative and qualitative nature of the humoral response.
Journal of immunology (Baltimore, Md. : 1950)187(7)3565-77(2011 Oct)
King RG,Herrin BR,Justement LB

The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes.
Journal of immunology (Baltimore, Md. : 1950)179(3)1768-75(2007 Aug)
Perchonock CE,Pajerowski AG,Nguyen C,Shapiro MJ,Shapiro VS

Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX.
Molecular and cellular biology26(16)6005-15(2006 Aug)
Perchonock CE,Fernando MC,Quinn WJ 3rd,Nguyen CT,Sun J,Shapiro MJ,Shapiro VS

Expression of the adaptor protein hematopoietic Src homology 2 is up-regulated in response to stimuli that promote survival and differentiation of B cells.
Journal of immunology (Baltimore, Md. : 1950)176(7)4163-72(2006 Apr)
Herrin BR,Justement LB

The adaptor protein HSH2 attenuates apoptosis in response to ligation of the B cell antigen receptor complex on the B lymphoma cell line, WEHI-231.
The Journal of biological chemistry280(5)3507-15(2005 Feb)
Herrin BR,Groeger AL,Justement LB

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Hsh2d cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報