Taf3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Taf3
Entrez Gene ID	209361
Full Name	TATA-box binding protein associated factor 3
Synonyms	140kDa,4933439M23Rik,AW539625,TAF140,TAFII-140,TAFII140,mTAFII140
General protein information	Preferred Names TATA-box binding protein associated factor 3 Names transcription initiation factor TFIID subunit 3 140 kDa TATA box-binding protein-associated factor RNA polymerase II transcription factor, TFIID subunit TAF(II)140 TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa TBP-associated factor 3 transcription initiation factor TFIID 140 kDa subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_027748.3	NP_082024.2	transcription initiation factor TFIID subunit 3

Pathways from BioSystems

Homologies

	LOC722623	XP_002808540.1
Bos taurus (cattle)	LOC506674	XP_003586802.1
Xenopus tropicalis (tropical clawed frog)	taf3	XP_004913100.1
Rattus norvegicus (Norway rat)	Taf3	NP_001258271.1
Mus musculus (house mouse)	Taf3	NP_082024.2
Canis lupus familiaris (dog)	TAF3	XP_544263.2
Homo sapiens (human)	TAF3	NP_114129.1
Pan troglodytes (chimpanzee)	TAF3	XP_001157768.2
Gallus gallus (chicken)	TAF3	NP_001026012.1

Gene Ontology
Bibliography

Related articles in PubMed

The TAF10-containing TFIID and SAGA transcriptional complexes are dispensable for early somitogenesis in the mouse embryo.
Bardot P, Vincent SD, Fournier M, Hubaud A, Joint M, Tora L, Pourqui? O
Development (Cambridge, England)144(20)3808-3818(2017 10)

Essential role of the TFIID subunit TAF4 in murine embryogenesis and embryonic stem cell differentiation.
Langer D, Martianov I, Alpern D, Rhinn M, Keime C, Doll? P, Mengus G, Davidson I
Nature communications711063(2016 Mar)

The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output.
Stijf-Bultsma Y, Sommer L, Tauber M, Baalbaki M, Giardoglou P, Jones DR, Gelato KA, van Pelt J, Shah Z, Rahnamoun H, Toma C, Anderson KE, Hawkins P, Lauberth SM, Haramis AP, Hart D, Fischle W, Divecha N
Molecular cell58(3)453-67(2015 May)

Defective transcription initiation causes postnatal growth failure in a mouse model of nucleotide excision repair (NER) progeria.
Kamileri I, Karakasilioti I, Sideri A, Kosteas T, Tatarakis A, Talianidis I, Garinis GA
Proceedings of the National Academy of Sciences of the United States of America109(8)2995-3000(2012 Feb)

Control of embryonic stem cell lineage commitment by core promoter factor, TAF3.
Liu Z, Scannell DR, Eisen MB, Tjian R
Cell146(5)720-31(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that TATA box binding protein-associated factor TAF3 as a direct target of nuclear phosphoinositides (PI).
Title: The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output.

Study shows that this TAF3 activity is critical for early lineage segregation during stem cell differentiation.
Title: Control of embryonic stem cell lineage commitment by core promoter factor, TAF3.

In vitro and cell-based assays identify a domain of TAF3 that mediates coactivator functions targeted by MyoD.
Title: MyoD targets TAF3/TRF3 to activate myogenin transcription.

Results report the solution structure of the TAF3-PHD finger and its complex with an H3K4me3 peptide.
Title: Structural insight into the recognition of the H3K4me3 mark by the TFIID subunit TAF3.

The following Taf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Taf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15670	NM_027748.3 Latest version!	Mus musculus TATA-box binding protein associated factor 3 (Taf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$545.30 $779.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Taf3 ( NM_027748.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082024.2 Mus musculus TATA-box binding protein associated factor 3 (Taf3), mRNA.

Price & Availability↑

CloneID	OMu15670
Clone ID Related Accession (Same CDS sequence)	NM_027748.3
Accession Version	NM_027748.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	transcription initiation factor TFIID subunit 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB621106.1, BC137618.1, AK136045.1, CX763644.1 and AW049595.1. On Oct 30, 2009 this sequence version replaced NM_027748.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC137617.1, AK154372.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

ATGTGCGAGA GTTACTCTAG GTCGTTGTTG AGGGTCTCGG TGGCGCAGAT CTGCCAGGCG 
CTGGGCTGGG ACTCGGTGCA GCTCAGCGCC TGCCACCTCC TCACCGACGT CCTGCAGCGC 
TATCTGCAGC AGCTGGGCCG GGGCTGCCAT CGGTACTCTG AACTCTATGG CCGAACAGAC 
CCCATTTTGG ATGATGTTGG TGAAGCCTTC CAACTTATGG GAGTTAATCT GCATGAACTA 
GAAGACTATA TTCATAATAT TGAACCTGTC ACTTTCCCAC ATCAAATTCC TTCATTTCCT 
GTTAGCAAGA ACAATGTCCT TCAGTTTCCT CAGCCCGGAA GTAAAGATGC AGAGGAAAGA 
AAAGACTACA TTCCGGATTA CCTTCCCCCC ATCGTGTCTT CTCAAGAAGA GGAGGAAGAG 
GAACAGGTGC CCACTGATGG GGGCACCTCA GCAGAAGCCA TGCAGGTGCC CTTGGAAGAA 
GATGATGAGA TGGAGGAGGA GGAAGTCATC AATGATGAGA ACTTCTTGGG TAAGAGGCCA 
CTGGACAGTC CTGAAGTGGA AGAAATGCCC TCCATGAAGC GCCCACGACT CCTGAGCACT 
AAAGGGGACT CCCTAGATGT TGTGTTGTTA GAAGCACGAG AACCCCTCAG CTCTATAAAC 
CCACAGAAGA CCCCACCAGT GCTCTCTCCT GTGCGTGTCC AGGACAGGGC TGATCTGGCC 
CCTCCATCAC CACAGCCTCC CATGCTGGCT CCATTTGCAA AGTCACAACT ACCAATTGCA 
AAGCCGTTAG AAACAAAGTC GTTTACACCA AAAACAAAGA CTAAAGCTAG CTCTCCAGGT 
CAGAAAACTA AGTCACCCAA GGCTGCCCTA TCACCAGCAA GGCTTGGAAG TCCTATTCGG 
TCACCAAAAA CCATCCCAAA AGAAAAGAAG TCACCTGGAC GCTCCAAGAG CCCCAAGAGT 
CCTAAGAGCC CCAAGATTGT AGCTCATGTT CCCCAAACCC CTGTCAGACC TGAAACACCA 
AATAGGACCC CTTCAGCCAT GGTGGTTGAA AAAACTGTTA AAGAGACCAT CCCAGTGATG 
AAACCAACAC AGACTCCCCC AGAAGTTGTG AAACTGAACA TTGAAATGCA GCCGAAAAAG 
CCTGTGGTGA CAGACAAAAC CATCGATGAC TCCATCGATG CTGTGATCGC ACGTGCCTGT 
GCAGAGCGGG AGCCAGACCC TTTCGAGTTT TCTTCCGGAT CGGAATCGGA AGGAGACACT 
TTTACAAGTC CTAAGAGGAT TTCAGGCTCA GAGTGCGCCA CTCCAAAAGC CTCCACTTCC 
TCCAATAACT TCACCAAGTC CCTGGCCACT CCTCTGCCTC TCTCCAGCGG AACCTCAAGT 
TCAGATAACT CGTGGACAAT GGATGCCTCT ATTGATGAGG TGGTACGGAA GGCAAAGCTG 
GGAGCCCCTT CCAACATGCC TCCCACCTTC CCATATATCT CTTCTCCCTC AATTTCTCCT 
CCCACTCCTG AGCCTCTACA CAAGGGGTAT GAGGAGAAAG CCAAGCTGCC TTCGTCTGTG 
GATGTAAAGA AAAAGTTGAA AAAAGAACTG AAGACTAAGT TGAAAAAGAA AGAAAAGCAG 
CGAGACAGAG AGAGGGAGAG AGAGAGAAAC AAAGAAAGAA GCAAAGAGAA AGATAAAATG 
AGAGAGAGGG AGAAGGAGAA GGAGGCTGGA AAGGAACTTA AGTACCCGTG GAGGGAGCTG 
ATGAAAGACG AAGATTCTGA TCCCTATAAG TTTAAAATCA AAGAATTTGA AGACATTGAT 
GCTGCGAAAG TGCGATTGAA AGATGGGATC GTGAGGAGAG AGCGAGAGAA ACATAAGGAC 
AAGAAGAAAG ATCGAGAAAG AAGCAAGCGG GAGAAAGATA AGCGAGAAAG GGAAAGGCTT 
AAAGAAAAAA ACAGAGAAGA CAAGATAAAG GCCCCTCCAA CACAGCTGGT GTTGCCCCCA 
AAAGAAATGG CACTGCCTTT GTTCAGCCCT TCAGCAGTGA GGGTCCCGGC CATGCTGCCG 
GCCTTCTCAC CAATGCTCCC AGAAAAACTG TTTGAGGAAA AAGAGAAGCC CAAGGAGAAA 
GAAAGGAAAA AGGACAAAAA GGAGAAGAAG AAGAAGAAAG AGAAGGAGAA AGAAAAGGAG 
AAGAAAGAGA GGGAGAGGGA GAAGGAGAGA AGGGAGAGAG AGAAGAGAGA GAAGGAGAAG 
GAGAAGCACA AACATGAGAA GATAAAAGTG GAGCCTGTCA TCCCTGCCCC AAGTCCAGTT 
ATCCCCAGGT TGACTCTGCG AGTTGGCGCT GGCCAGGACA AGATTGTTAT CAGCAAGGTG 
GTACCAGCCC CGGAGGCTAA GCCCGCTCCT TCTCTGAACA GACCCAAAAC CCCACCTCCA 
GCCCCAGTCC CTATTCCTGT GCGAGTGAGC CCTACTCCCC TGCAGCCACC ACTCCTCACC 
CAGGCTGCAG TGTGCCCAGC CCTGATGCCA TCCCCAGCTC CCGCCCTCTC TGGAATAGGG 
AGTGCCAAAG CCCCCGTGAG GAGCGTCGTG ACTGAGACGG TCAGCACTTA TGTGATCCGT 
GATGAGTGGG GCAATCAGAT CTGGATCTGT CCAGGGTGCA ACAAGCCTGA TGACGGGAGC 
CCGATGATTG GCTGTGACGA CTGTGACGAT TGGTACCACT GGCCCTGTGT GGGAATCATG 
GCTGCTCCTC CAGAAGAGAT GCAGTGGTTC TGCCCGAAGT GTGCCAACAA GATAAAGAAA 
GACAAGAAGC ACAAGAAGAG GAAGCACCGT GCGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082024.2
CDS	210..3008
Translation	MCESYSRSLLRVSVAQICQALGWDSVQLSACHLLTDVLQRYLQQLGRGCHRYSELYGRTDPILDDVGEAFQLMGVNLHELEDYIHNIEPVTFPHQIPSFPVSKNNVLQFPQPGSKDAEERKDYIPDYLPPIVSSQEEEEEEQVPTDGGTSAEAMQVPLEEDDEMEEEEVINDENFLGKRPLDSPEVEEMPSMKRPRLLSTKGDSLDVVLLEAREPLSSINPQKTPPVLSPVRVQDRADLAPPSPQPPMLAPFAKSQLPIAKPLETKSFTPKTKTKASSPGQKTKSPKAALSPARLGSPIRSPKTIPKEKKSPGRSKSPKSPKSPKIVAHVPQTPVRPETPNRTPSAMVVEKTVKETIPVMKPTQTPPEVVKLNIEMQPKKPVVTDKTIDDSIDAVIARACAEREPDPFEFSSGSESEGDTFTSPKRISGSECATPKASTSSNNFTKSLATPLPLSSGTSSSDNSWTMDASIDEVVRKAKLGAPSNMPPTFPYISSPSISPPTPEPLHKGYEEKAKLPSSVDVKKKLKKELKTKLKKKEKQRDRERERERNKERSKEKDKMREREKEKEAGKELKYPWRELMKDEDSDPYKFKIKEFEDIDAAKVRLKDGIVRREREKHKDKKKDRERSKREKDKRERERLKEKNREDKIKAPPTQLVLPPKEMALPLFSPSAVRVPAMLPAFSPMLPEKLFEEKEKPKEKERKKDKKEKKKKKEKEKEKEKKEREREKERREREKREKEKEKHKHEKIKVEPVIPAPSPVIPRLTLRVGAGQDKIVISKVVPAPEAKPAPSLNRPKTPPPAPVPIPVRVSPTPLQPPLLTQAAVCPALMPSPAPALSGIGSAKAPVRSVVTETVSTYVIRDEWGNQIWICPGCNKPDDGSPMIGCDDCDDWYHWPCVGIMAAPPEEMQWFCPKCANKIKKDKKHKKRKHRAH

Target ORF information:

RefSeq Version	NM_027748.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus TATA-box binding protein associated factor 3 (Taf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027748.3

ORF Insert Sequence:

ATGTGCGAGA GTTACTCTAG GTCGTTGTTG AGGGTCTCGG TGGCGCAGAT CTGCCAGGCG 
CTGGGCTGGG ACTCGGTGCA GCTCAGCGCC TGCCACCTCC TCACCGACGT CCTGCAGCGC 
TATCTGCAGC AGCTGGGCCG GGGCTGCCAT CGGTACTCTG AACTCTATGG CCGAACAGAC 
CCCATTTTGG ATGATGTTGG TGAAGCCTTC CAACTTATGG GAGTTAATCT GCATGAACTA 
GAAGACTATA TTCATAATAT TGAACCTGTC ACTTTCCCAC ATCAAATTCC TTCATTTCCT 
GTTAGCAAGA ACAATGTCCT TCAGTTTCCT CAGCCCGGAA GTAAAGATGC AGAGGAAAGA 
AAAGACTACA TTCCGGATTA CCTTCCCCCC ATCGTGTCTT CTCAAGAAGA GGAGGAAGAG 
GAACAGGTGC CCACTGATGG GGGCACCTCA GCAGAAGCCA TGCAGGTGCC CTTGGAAGAA 
GATGATGAGA TGGAGGAGGA GGAAGTCATC AATGATGAGA ACTTCTTGGG TAAGAGGCCA 
CTGGACAGTC CTGAAGTGGA AGAAATGCCC TCCATGAAGC GCCCACGACT CCTGAGCACT 
AAAGGGGACT CCCTAGATGT TGTGTTGTTA GAAGCACGAG AACCCCTCAG CTCTATAAAC 
CCACAGAAGA CCCCACCAGT GCTCTCTCCT GTGCGTGTCC AGGACAGGGC TGATCTGGCC 
CCTCCATCAC CACAGCCTCC CATGCTGGCT CCATTTGCAA AGTCACAACT ACCAATTGCA 
AAGCCGTTAG AAACAAAGTC GTTTACACCA AAAACAAAGA CTAAAGCTAG CTCTCCAGGT 
CAGAAAACTA AGTCACCCAA GGCTGCCCTA TCACCAGCAA GGCTTGGAAG TCCTATTCGG 
TCACCAAAAA CCATCCCAAA AGAAAAGAAG TCACCTGGAC GCTCCAAGAG CCCCAAGAGT 
CCTAAGAGCC CCAAGATTGT AGCTCATGTT CCCCAAACCC CTGTCAGACC TGAAACACCA 
AATAGGACCC CTTCAGCCAT GGTGGTTGAA AAAACTGTTA AAGAGACCAT CCCAGTGATG 
AAACCAACAC AGACTCCCCC AGAAGTTGTG AAACTGAACA TTGAAATGCA GCCGAAAAAG 
CCTGTGGTGA CAGACAAAAC CATCGATGAC TCCATCGATG CTGTGATCGC ACGTGCCTGT 
GCAGAGCGGG AGCCAGACCC TTTCGAGTTT TCTTCCGGAT CGGAATCGGA AGGAGACACT 
TTTACAAGTC CTAAGAGGAT TTCAGGCTCA GAGTGCGCCA CTCCAAAAGC CTCCACTTCC 
TCCAATAACT TCACCAAGTC CCTGGCCACT CCTCTGCCTC TCTCCAGCGG AACCTCAAGT 
TCAGATAACT CGTGGACAAT GGATGCCTCT ATTGATGAGG TGGTACGGAA GGCAAAGCTG 
GGAGCCCCTT CCAACATGCC TCCCACCTTC CCATATATCT CTTCTCCCTC AATTTCTCCT 
CCCACTCCTG AGCCTCTACA CAAGGGGTAT GAGGAGAAAG CCAAGCTGCC TTCGTCTGTG 
GATGTAAAGA AAAAGTTGAA AAAAGAACTG AAGACTAAGT TGAAAAAGAA AGAAAAGCAG 
CGAGACAGAG AGAGGGAGAG AGAGAGAAAC AAAGAAAGAA GCAAAGAGAA AGATAAAATG 
AGAGAGAGGG AGAAGGAGAA GGAGGCTGGA AAGGAACTTA AGTACCCGTG GAGGGAGCTG 
ATGAAAGACG AAGATTCTGA TCCCTATAAG TTTAAAATCA AAGAATTTGA AGACATTGAT 
GCTGCGAAAG TGCGATTGAA AGATGGGATC GTGAGGAGAG AGCGAGAGAA ACATAAGGAC 
AAGAAGAAAG ATCGAGAAAG AAGCAAGCGG GAGAAAGATA AGCGAGAAAG GGAAAGGCTT 
AAAGAAAAAA ACAGAGAAGA CAAGATAAAG GCCCCTCCAA CACAGCTGGT GTTGCCCCCA 
AAAGAAATGG CACTGCCTTT GTTCAGCCCT TCAGCAGTGA GGGTCCCGGC CATGCTGCCG 
GCCTTCTCAC CAATGCTCCC AGAAAAACTG TTTGAGGAAA AAGAGAAGCC CAAGGAGAAA 
GAAAGGAAAA AGGACAAAAA GGAGAAGAAG AAGAAGAAAG AGAAGGAGAA AGAAAAGGAG 
AAGAAAGAGA GGGAGAGGGA GAAGGAGAGA AGGGAGAGAG AGAAGAGAGA GAAGGAGAAG 
GAGAAGCACA AACATGAGAA GATAAAAGTG GAGCCTGTCA TCCCTGCCCC AAGTCCAGTT 
ATCCCCAGGT TGACTCTGCG AGTTGGCGCT GGCCAGGACA AGATTGTTAT CAGCAAGGTG 
GTACCAGCCC CGGAGGCTAA GCCCGCTCCT TCTCTGAACA GACCCAAAAC CCCACCTCCA 
GCCCCAGTCC CTATTCCTGT GCGAGTGAGC CCTACTCCCC TGCAGCCACC ACTCCTCACC 
CAGGCTGCAG TGTGCCCAGC CCTGATGCCA TCCCCAGCTC CCGCCCTCTC TGGAATAGGG 
AGTGCCAAAG CCCCCGTGAG GAGCGTCGTG ACTGAGACGG TCAGCACTTA TGTGATCCGT 
GATGAGTGGG GCAATCAGAT CTGGATCTGT CCAGGGTGCA ACAAGCCTGA TGACGGGAGC 
CCGATGATTG GCTGTGACGA CTGTGACGAT TGGTACCACT GGCCCTGTGT GGGAATCATG 
GCTGCTCCTC CAGAAGAGAT GCAGTGGTTC TGCCCGAAGT GTGCCAACAA GATAAAGAAA 
GACAAGAAGC ACAAGAAGAG GAAGCACCGT GCGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The TAF10-containing TFIID and SAGA transcriptional complexes are dispensable for early somitogenesis in the mouse embryo.
Development (Cambridge, England)144(20)3808-3818(2017 10)
Bardot P,Vincent SD,Fournier M,Hubaud A,Joint M,Tora L,Pourqui? O

Essential role of the TFIID subunit TAF4 in murine embryogenesis and embryonic stem cell differentiation.
Nature communications711063(2016 Mar)
Langer D,Martianov I,Alpern D,Rhinn M,Keime C,Doll? P,Mengus G,Davidson I

The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output.
Molecular cell58(3)453-67(2015 May)
Stijf-Bultsma Y,Sommer L,Tauber M,Baalbaki M,Giardoglou P,Jones DR,Gelato KA,van Pelt J,Shah Z,Rahnamoun H,Toma C,Anderson KE,Hawkins P,Lauberth SM,Haramis AP,Hart D,Fischle W,Divecha N

Defective transcription initiation causes postnatal growth failure in a mouse model of nucleotide excision repair (NER) progeria.
Proceedings of the National Academy of Sciences of the United States of America109(8)2995-3000(2012 Feb)
Kamileri I,Karakasilioti I,Sideri A,Kosteas T,Tatarakis A,Talianidis I,Garinis GA

Control of embryonic stem cell lineage commitment by core promoter factor, TAF3.
Cell146(5)720-31(2011 Sep)
Liu Z,Scannell DR,Eisen MB,Tjian R