gRNAs
The following Slk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu55353 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006526827.4 , XM_006526827.3 | |
| Accession Version | XM_006526827.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | STE20-like serine/threonine-protein kinase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526827.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGTTGAGA TTGACATATT AGCATCTTGT GATCACCCAA ACATCGTCAA GCTTCTAGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006526890.1 |
| CDS | 251..3724 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_006526827.4 |
1 | ATGGTTGAGA TTGACATATT AGCATCTTGT GATCACCCAA ACATCGTCAA GCTTCTAGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu03883 | |
| Clone ID Related Accession (Same CDS sequence) | NM_009289.3 | |
| Accession Version | NM_009289.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-07-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | STE20-like serine/threonine-protein kinase isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125075.4, AK029491.1, CF748583.1, CA535951.1, CO431234.1, AK037798.1, AK051969.1 and CD740818.1. On Sep 9, 2009 this sequence version replaced NM_009289.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF039574.1, BC131675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTCCTTCT TCAATTTCCG TAAGATCTTC AAGTTGGGGA GCGAGAAGAA GAAGAAACAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_033315.2 |
| CDS | 176..3877 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_009289.3 |
1 | ATGTCCTTCT TCAATTTCCG TAAGATCTTC AAGTTGGGGA GCGAGAAGAA GAAGAAACAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu09970 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001164639.1 | |
| Accession Version | NM_001164639.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-07-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | STE20-like serine/threonine-protein kinase isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125075.4, AK029491.1, AK037798.1, CO431234.1, AK051969.1 and CD740818.1. Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122218.1, AF112855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTCCTTCT TCAATTTCCG TAAGATCTTC AAGTTGGGGA GCGAGAAGAA GAAGAAACAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001158111.1 |
| CDS | 176..3784 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001164639.1 |
1 | ATGTCCTTCT TCAATTTCCG TAAGATCTTC AAGTTGGGGA GCGAGAAGAA GAAGAAACAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu55353 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006526827.4 , XM_006526827.3 | |
| Accession Version | XM_006526827.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | STE20-like serine/threonine-protein kinase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGTTGAGA TTGACATATT AGCATCTTGT GATCACCCAA ACATCGTCAA GCTTCTAGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006526890.1 |
| CDS | 252..3725 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006526827.3 |
1 | ATGGTTGAGA TTGACATATT AGCATCTTGT GATCACCCAA ACATCGTCAA GCTTCTAGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Ste20-like Kinase, SLK, a Novel Mediator of Podocyte Integrity. |
Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness. |
Recruitment and activation of SLK at the leading edge of migrating cells requires Src family kinase activity and the LIM-only protein 4. |
SLK-dependent activation of ERMs controls LGN-NuMA localization and spindle orientation. |
A combination of SILAC and nucleotide acyl phosphate labelling reveals unexpected targets of the Rsk inhibitor BI-D1870. |
