Eif4g1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif4g1
Entrez Gene ID	208643
Full Name	eukaryotic translation initiation factor 4, gamma 1
Synonyms	E030015G23Rik,eIF-4-gamma 1,eIF-4G 1,eIF-4G1,eIF4GI
General protein information	Preferred Names eukaryotic translation initiation factor 4, gamma 1 Names eukaryotic translation initiation factor 4 gamma 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B1
Summary	This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017316920.1	XP_017172409.1	eukaryotic translation initiation factor 4 gamma 1 isoform X11
XM_006521937.1	XP_006522000.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2
XM_011245861.1	XP_011244163.1	eukaryotic translation initiation factor 4 gamma 1 isoform X5
XM_006521945.1	XP_006522008.1	eukaryotic translation initiation factor 4 gamma 1 isoform X11
NM_145941.3	NP_666053.2	eukaryotic translation initiation factor 4 gamma 1 isoform a
NM_001304432.1	NP_001291361.1	eukaryotic translation initiation factor 4 gamma 1 isoform c
XM_006521938.1	XP_006522001.1	eukaryotic translation initiation factor 4 gamma 1 isoform X3
XM_006521935.1	XP_006521998.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_006521942.1	XP_006522005.1	eukaryotic translation initiation factor 4 gamma 1 isoform X7
XM_030249025.1	XP_030104885.1	eukaryotic translation initiation factor 4 gamma 1 isoform X9
XM_017316919.1	XP_017172408.1	eukaryotic translation initiation factor 4 gamma 1 isoform X7
XM_006521939.1	XP_006522002.1	eukaryotic translation initiation factor 4 gamma 1 isoform X3
XM_006521936.2	XP_006521999.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_006521940.4	XP_006522003.1	eukaryotic translation initiation factor 4 gamma 1 isoform X4
XM_006521944.3	XP_006522007.1	eukaryotic translation initiation factor 4 gamma 1 isoform X10
XM_006521946.1	XP_006522009.1	eukaryotic translation initiation factor 4 gamma 1 isoform X12
XM_030249027.1	XP_030104887.1	eukaryotic translation initiation factor 4 gamma 1 isoform X13
XM_006521940.3	XP_006522003.1	eukaryotic translation initiation factor 4 gamma 1 isoform X4
XM_006521938.2	XP_006522001.1	eukaryotic translation initiation factor 4 gamma 1 isoform X3
XM_030249023.1	XP_030104883.1	eukaryotic translation initiation factor 4 gamma 1 isoform X6
XM_030249024.1	XP_030104884.1	eukaryotic translation initiation factor 4 gamma 1 isoform X8
XM_006521943.1	XP_006522006.1	eukaryotic translation initiation factor 4 gamma 1 isoform X9
XM_030249026.1	XP_030104886.1	eukaryotic translation initiation factor 4 gamma 1 isoform X10

Pathways from BioSystems

Homologies

	EIF4G1	XP_002802932.1
Canis lupus familiaris (dog)	EIF4G1	XP_005640005.1
Bos taurus (cattle)	EIF4G1	XP_003585711.2
Mus musculus (house mouse)	Eif4g1	NP_666053.2
Homo sapiens (human)	EIF4G1	NP_886553.3
Rattus norvegicus (Norway rat)	Eif4g1	XP_006248643.1
Gallus gallus (chicken)	EIF4G1	XP_004943468.1
Xenopus tropicalis (tropical clawed frog)	eif4g3	XP_004914456.1
Pan troglodytes (chimpanzee)	EIF4G1	XP_003310194.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential Requirements for eIF4E Dose in Normal Development and Cancer.
Truitt ML, Conn CS, Shi Z, Pang X, Tokuyasu T, Coady AM, Seo Y, Barna M, Ruggero D
Cell162(1)59-71(2015 Jul)

Parkinson's disease genes VPS35 and EIF4G1 interact genetically and converge on ?-synuclein.
Dhungel N, Eleuteri S, Li LB, Kramer NJ, Chartron JW, Spencer B, Kosberg K, Fields JA, Stafa K, Adame A, Lashuel H, Frydman J, Shen K, Masliah E, Gitler AD
Neuron85(1)76-87(2015 Jan)

An eIF4E1/4E-T complex determines the genesis of neurons from precursors by translationally repressing a proneurogenic transcription program.
Yang G, Smibert CA, Kaplan DR, Miller FD
Neuron84(4)723-39(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our studies reveal unexpected genetic and functional interactions between VPS35 and EIF4G1, two seemingly unrelated PD genes, and functionally connect them to alpha-synuclein pathobiology in yeast, worms, and mouse.
Title: Parkinson's disease genes VPS35 and EIF4G1 interact genetically and converge on ?-synuclein.

Disruption of insulin receptor (IR) expression in beta cells has a direct impact on the expression of the convertase enzyme carboxypeptidase E (CPE) by inhibition of the eukaryotic translation initiation factor Eif4g1.
Title: Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic ? cells.

Data indicate that MNK2 plays a unique role, not shared by its closest paralog MNK1, in limiting protein translation through its negative effect on eIF4G Ser1108 phosphorylation and p70S6K activation.
Title: MNK2 inhibits eIF4G activation through a pathway involving serine-arginine-rich protein kinase in skeletal muscle.

expression of 3e5 diminishes eIF4G interaction with eIF3 and causes abnormal gene expression at the translational level.
Title: Expression of truncated eukaryotic initiation factor 3e (eIF3e) resulting from integration of mouse mammary tumor virus (MMTV) causes a shift from cap-dependent to cap-independent translation.

findings assign NAD(P)H quinone-oxydoreductase 1 an original role in the regulation of mRNA translation via the control of eIF4GI stability by the proteasome.
Title: NAD(P)H quinone-oxydoreductase 1 protects eukaryotic translation initiation factor 4GI from degradation by the proteasome.

The following Eif4g1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif4g1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu79292	XM_017316920.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OMu86907	XM_030249027.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu72647	NM_001304432.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$811.30 $1159.00
OMu14490	NM_145941.3 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$811.30 $1159.00
OMu14490	XM_006521937.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$811.30 $1159.00
OMu14547	XM_006521940.4 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu51987	XM_006521938.2 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu51990	XM_030249025.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu51991	XM_030249026.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu86905	XM_030249023.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu86906	XM_030249024.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu51986	XM_006521935.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu51986	XM_006521936.2 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu51987	XM_006521939.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu51989	XM_006521942.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu51990	XM_006521943.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu51990	XM_017316919.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu51991	XM_006521944.3 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu51992	XM_006521945.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu51993	XM_006521946.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu72646	XM_011245861.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu14547	XM_006521940.3	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu51987	XM_006521938.1	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif4g1 ( XM_017316920.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172409.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA.

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CloneID	OMu79292
Clone ID Related Accession (Same CDS sequence)	XM_017316920.1
Accession Version	XM_017316920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT 
GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA 
GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC 
ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG 
GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT 
GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA 
GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG 
AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA 
GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA 
GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC 
CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC 
CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC 
TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG 
TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC 
AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG 
CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT 
CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT 
GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC TCTCAGCAGG GCCCACGAAA GGAAACTCGC 
AAGATCATTT CCTCAGTGAT AATGACTGAA GACATAAAAC TAAATAAAGC AGAGAAGGCT 
TGGAAACCCA GTAGCAAACG GACAGCAGCT GATAAGGATC GAGGGGAAGA GGATGCTGAT 
GGAAGCAAGA CCCAGGACCT GTTCCGCAGG GTACGCTCCA TCCTGAATAA GCTGACACCC 
CAGATGTTTC AGCAGCTGAT GAAGCAGGTG ACACAGCTGG CCATTGACAC GGAGGAACGC 
CTCAAAGGAG TTATTGACCT CATCTTTGAG AAAGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCTTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTAACTG TGAATTTTCG AAAACTGTTG TTAAATCGGT GCCAGAAGGA ATTTGAGAAA 
GACAAAGATG ATGATGAAGT TTTTGAAAAA AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAAGAAC GGGGACGCCT GAAAGAAGAG CTAGAAGAAG CCCGGGACAT AGCTAGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTATTCA AGTTGAAGAT GTTAACAGAA 
GCAATAATGC ATGACTGTGT GGTCAAGCTA CTTAAGAACC ATGATGAGGA GTCCCTGGAA 
TGCCTCTGCC GCCTCCTCAC CACTATAGGC AAAGACCTGG ACTTTGCAAA AGCCAAGCCT 
CGAATGGATC AGTATTTCAA CCAAATGGAA AAAATCATTA AAGAAAAGAA GACCTCATCT 
CGCATCCGTT TTATGCTGCA GGATGTACTC GATCTGCGGC AGAGTAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCTAA GACTATTGAT CAAATTCACA AGGAGGCTGA GATGGAAGAG 
CACCGAGAAC ATATTAAAGT ACAGCAGCTC ATGGCCAAGG GCAGCGACAA ACGTCGGGGT 
GGCCCTCCAG GCCCGCCTAT CAACCGTGGC CTTCCACTTG TAGATGATGG TGGCTGGAAT 
ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC ATTGACACCT CACGGCTCAC CAAGATCACA 
AAGCCTGGTT CCATTGATTC TAACAACCAG CTTTTTGCAC CTGGAGGACG ACTGAGTTGG 
GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC AAGCCCTCAG ACACAGCATC GGAAGCTACT 
CGTCCAGCTA CCTTGAATCG CTTTTCTGCT CTTCAACAAA CATTACCCGC AGAGAACACA 
GATAACAGAC GTGTTGTACA GAGGAGTAGC TTGAGCCGGG AACGAGGTGA GAAAGCTGGG 
GACCGGGGAG ACCGACTAGA GCGGAGTGAG CGGGGAGGTG ACCGTGGGGA CCGACTTGAT 
CGTGCCAGAA CACCTGCCAC CAAGCGAAGT TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA 
GAGCGGCCAT CCCAGCCTGA GGGACTCCGC AAGGCAGCTA GCCTCACAGA GGATCGTGGT 
CGGGATCCTG TGAAGCGGGA AGCCACTCTA CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG 
TCTGTGGATG AGGTGGAGAA GAAATCTAAG GCCATCATTG AGGAATATCT CCATCTCAAT 
GACATGAAGG AGGCAGTACA GTGTGTCCAG GAGCTGGCTT CACCCTCCTT GCTCTTCATC 
TTCGTACGGC TTGGTATCGA GTCCACGCTG GAGCGTAGTA CCATTGCTCG TGAGCATATG 
GGGCGACTAC TGCACCAACT GCTCTGTGCG GGGCACCTCT CTACTGCCCA GTACTATCAA 
GGGCTGTATG AAACACTAGA ATTGGCTGAG GACATGGAAA TTGATATCCC TCATGTATGG 
CTTTACCTGG CAGAACTGAT TACACCTATT CTCCAGGAAG ATGGGGTGCC CATGGGAGAG 
CTCTTCAGGG AAATTACGAA GCCTCTGAGA CCCATGGGCA AAGCTACTTC TTTATTGCTG 
GAGATCCTGG GTCTCTTATG CAAGAGCATG GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA 
GAGGCTGGGC TGAGCTGGAG GGAATTTCTA GCAGAGGGCC AAGACGTTGG CTCATTTGTG 
GCTGAAAAGA AGGTGGAATA TACCTTGGGA GAAGAATCTG AAGCTCCTGG CCAGAGGACA 
CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA GAGAAGCTGC TGAAGGACGG CGGCAGTAAT 
CAGCGTGTGT TCGACTGGAT AGACGCCAAC CTAAATGAGC AGCAGATAGC ATCCAATACA 
TTAGTTCGAG CCCTCATGAC AACTGTCTGT TATTCTGCAA TTATCTTTGA GACTCCTCTC 
CGAGTGGATG TTCAAGTGTT GAAAGTGCGA GCAAGACTGC TGCAGAAATA CCTGTGTGAT 
GAGCAGAAGG AGCTACAAGC ACTCTATGCT CTCCAGGCCC TTGTAGTGAC CTTAGAACAG 
CCTGCCAACC TGCTTCGGAT GTTCTTTGAT GCTCTATATG ATGAGGACGT GGTGAAGGAA 
GACGCCTTCT ACAGCTGGGA GAGCAGCAAG GATCCTGCTG AACAGCAGGG CAAGGGTGTG 
GCCCTTAAAT CTGTCACAGC ATTCTTCAAT TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT 
GATCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172409.1
CDS	1143..5054
Translation	MTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_017316920.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316920.1

ORF Insert Sequence:

ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT 
GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA 
GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC 
ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG 
GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT 
GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA 
GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG 
AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA 
GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA 
GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC 
CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC 
CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC 
TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG 
TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC 
AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG 
CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT 
CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT 
GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC TCTCAGCAGG GCCCACGAAA GGAAACTCGC 
AAGATCATTT CCTCAGTGAT AATGACTGAA GACATAAAAC TAAATAAAGC AGAGAAGGCT 
TGGAAACCCA GTAGCAAACG GACAGCAGCT GATAAGGATC GAGGGGAAGA GGATGCTGAT 
GGAAGCAAGA CCCAGGACCT GTTCCGCAGG GTACGCTCCA TCCTGAATAA GCTGACACCC 
CAGATGTTTC AGCAGCTGAT GAAGCAGGTG ACACAGCTGG CCATTGACAC GGAGGAACGC 
CTCAAAGGAG TTATTGACCT CATCTTTGAG AAAGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCTTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTAACTG TGAATTTTCG AAAACTGTTG TTAAATCGGT GCCAGAAGGA ATTTGAGAAA 
GACAAAGATG ATGATGAAGT TTTTGAAAAA AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAAGAAC GGGGACGCCT GAAAGAAGAG CTAGAAGAAG CCCGGGACAT AGCTAGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTATTCA AGTTGAAGAT GTTAACAGAA 
GCAATAATGC ATGACTGTGT GGTCAAGCTA CTTAAGAACC ATGATGAGGA GTCCCTGGAA 
TGCCTCTGCC GCCTCCTCAC CACTATAGGC AAAGACCTGG ACTTTGCAAA AGCCAAGCCT 
CGAATGGATC AGTATTTCAA CCAAATGGAA AAAATCATTA AAGAAAAGAA GACCTCATCT 
CGCATCCGTT TTATGCTGCA GGATGTACTC GATCTGCGGC AGAGTAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCTAA GACTATTGAT CAAATTCACA AGGAGGCTGA GATGGAAGAG 
CACCGAGAAC ATATTAAAGT ACAGCAGCTC ATGGCCAAGG GCAGCGACAA ACGTCGGGGT 
GGCCCTCCAG GCCCGCCTAT CAACCGTGGC CTTCCACTTG TAGATGATGG TGGCTGGAAT 
ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC ATTGACACCT CACGGCTCAC CAAGATCACA 
AAGCCTGGTT CCATTGATTC TAACAACCAG CTTTTTGCAC CTGGAGGACG ACTGAGTTGG 
GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC AAGCCCTCAG ACACAGCATC GGAAGCTACT 
CGTCCAGCTA CCTTGAATCG CTTTTCTGCT CTTCAACAAA CATTACCCGC AGAGAACACA 
GATAACAGAC GTGTTGTACA GAGGAGTAGC TTGAGCCGGG AACGAGGTGA GAAAGCTGGG 
GACCGGGGAG ACCGACTAGA GCGGAGTGAG CGGGGAGGTG ACCGTGGGGA CCGACTTGAT 
CGTGCCAGAA CACCTGCCAC CAAGCGAAGT TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA 
GAGCGGCCAT CCCAGCCTGA GGGACTCCGC AAGGCAGCTA GCCTCACAGA GGATCGTGGT 
CGGGATCCTG TGAAGCGGGA AGCCACTCTA CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG 
TCTGTGGATG AGGTGGAGAA GAAATCTAAG GCCATCATTG AGGAATATCT CCATCTCAAT 
GACATGAAGG AGGCAGTACA GTGTGTCCAG GAGCTGGCTT CACCCTCCTT GCTCTTCATC 
TTCGTACGGC TTGGTATCGA GTCCACGCTG GAGCGTAGTA CCATTGCTCG TGAGCATATG 
GGGCGACTAC TGCACCAACT GCTCTGTGCG GGGCACCTCT CTACTGCCCA GTACTATCAA 
GGGCTGTATG AAACACTAGA ATTGGCTGAG GACATGGAAA TTGATATCCC TCATGTATGG 
CTTTACCTGG CAGAACTGAT TACACCTATT CTCCAGGAAG ATGGGGTGCC CATGGGAGAG 
CTCTTCAGGG AAATTACGAA GCCTCTGAGA CCCATGGGCA AAGCTACTTC TTTATTGCTG 
GAGATCCTGG GTCTCTTATG CAAGAGCATG GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA 
GAGGCTGGGC TGAGCTGGAG GGAATTTCTA GCAGAGGGCC AAGACGTTGG CTCATTTGTG 
GCTGAAAAGA AGGTGGAATA TACCTTGGGA GAAGAATCTG AAGCTCCTGG CCAGAGGACA 
CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA GAGAAGCTGC TGAAGGACGG CGGCAGTAAT 
CAGCGTGTGT TCGACTGGAT AGACGCCAAC CTAAATGAGC AGCAGATAGC ATCCAATACA 
TTAGTTCGAG CCCTCATGAC AACTGTCTGT TATTCTGCAA TTATCTTTGA GACTCCTCTC 
CGAGTGGATG TTCAAGTGTT GAAAGTGCGA GCAAGACTGC TGCAGAAATA CCTGTGTGAT 
GAGCAGAAGG AGCTACAAGC ACTCTATGCT CTCCAGGCCC TTGTAGTGAC CTTAGAACAG 
CCTGCCAACC TGCTTCGGAT GTTCTTTGAT GCTCTATATG ATGAGGACGT GGTGAAGGAA 
GACGCCTTCT ACAGCTGGGA GAGCAGCAAG GATCCTGCTG AACAGCAGGG CAAGGGTGTG 
GCCCTTAAAT CTGTCACAGC ATTCTTCAAT TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT 
GATCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_030249027.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104887.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X17, mRNA.

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CloneID	OMu86907
Clone ID Related Accession (Same CDS sequence)	XM_030249027.1
Accession Version	XM_030249027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATCC CTTCCCAGAT CTCCTACTCA GCCTCCCAAG GAGCCTACTA TATCCCTGGA 
CAGCAGTACC CTGTGCAGCC AGGAGCCCCA GGCTTCTATC CAGGTGCAAG CCCTACCGAG 
TTTGGGACCT ATGCTGGAGC CTATTACCCA GCCCAAGGTG TGCAGCAGTT TCCTGCTAGT 
GTGGCTCCTG CCCCAGTTTT GATGAACCAG CCACCCCAGA TTGCTCCTAA GAGGGAACGG 
AAAACTATCC GAATTCGAGA CCCAAACCAA GGAGGGAAGG ATATCACAGA AGAGATCATG 
TCTGGGGCCC GCACTGCCTC CACACCCACT CCTCCCCAGA CGGGAGGCAG TCTGGAGCCT 
CAACCCAATG GGGAGTCGCC TCAGGTTGCT GTCATTATCC GGCCAGATGA CCGGTCGCAG 
GGAGCAGCCA TTGGGGGGCG GCCAGGACTG CCTGGCCCAG AGCATAGCCC TGGCACAGAA 
TCTCAGCCTT CGTCGCCTTC TCCAACCCCA TCACCACCCC CAATTTTGGA GCCGGGGTCT 
GAGTCTAATC TTGGAGTCCT CTCTATTCCT GGGGACACTA TGACAACAGG GATGATACCA 
ATGTCTGTAG AAGAATCGAC CCCTATCTCT TGTGAAACTG GGGAGCCGTA TTGCCTCTCT 
CCAGAACCCA CTCTTGCCGA ACCCATACTG GAAGTAGAAG TGACACTCAG CAAACCCATT 
CCAGAATCTG AGTTCTCTTC CAGTCCTCTC CAGGTTTCCA CGGCCCTGGT GCCTCACAAG 
GTTGAAACTC ATGAGCCCAA TGGCGTGATC CCATCTGAGG ATCTGGAACC AGAGGTGGAG 
TCAAGCACAG AGCCAGCTCC TCCCCCTCTA TCACCTTGTG CTTCTGAATC GCTCGTGCCC 
ATTGCTCCAA CTGCCCAGCC TGAGGAACTG CTCAACGGAG CCCCCTCACC ACCAGCTGTG 
GATTTAAGCC CAGTCAGTGA GCCAGAGGAA CAGGCCAAGA AGGTTTCATC AGCAGCACTG 
GCCAGCATTC TCTCTCCTGC TCCACCTGTG GCTCCTTCAG ATACTTCTCC TGCTCAGGAG 
GAAGAAATGG AAGAAGATGA CGATGACGAA GAAGGTGGAG AAGCTGAGAG TGAGAAGGGA 
GGAGAGGACG TCCCCCTTGA CAGTACTCCT GTCCCAGCCC AGCTGTCTCA GAATTTAGAG 
GTGGCAGCAG CTACCCAGGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAAGAG 
CTAAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGT GGACCCAGCA 
GTACCAGAGG TAGAGAATCA GCCTCCCACT GGCAGCAACC CAAGCCCAGA GTCTGAGGGC 
AGCATGGTGC CCACACAGCC TGAGGAAACA GAAGAAACCT GGGACTCTAA GGAAGACAAA 
ATTCATAATG CTGAGAACAT CCAGCCTGGG GAGCAGAAGT ATGAGTACAA GTCAGATCAG 
TGGAAGCCTC TAAACCTTGA AGAAAAGAAG CGTTATGACA GAGAATTCCT GCTGGGCTTT 
CAGTTCATCT TTGCCAGCAT GCAGAAGCCA GAAGGATTGC CCCATATCAC TGATGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTTCGG CAACTGGATC CCTCCAGACT ACCTGGCATA 
AACTGTGGCC CAGATTTCAC TCCATCTTTT GCCAATCTTG GCCGACCAGC CCTCAGCAAC 
CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG 
GGACCCAGGC GCTCTCAGCA GGGCCCACGA AAGGAAACTC GCAAGATCAT TTCCTCAGTG 
ATAATGACTG AAGACATAAA ACTAAATAAA GCAGAGAAGG CTTGGAAACC CAGTAGCAAA 
CGGACAGCAG CTGATAAGGA TCGAGGGGAA GAGGATGCTG ATGGAAGCAA GACCCAGGAC 
CTGTTCCGCA GGGTACGCTC CATCCTGAAT AAGCTGACAC CCCAGATGTT TCAGCAGCTG 
ATGAAGCAGG TGACACAGCT GGCCATTGAC ACGGAGGAAC GCCTCAAAGG AGTTATTGAC 
CTCATCTTTG AGAAAGCCAT TTCAGAGCCC AACTTCTCTG TGGCTTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTAAC TGTGAATTTT 
CGAAAACTGT TGTTAAATCG GTGCCAGAAG GAATTTGAGA AAGACAAAGA TGATGATGAA 
GTTTTTGAAA AAAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAAGA ACGGGGACGC 
CTGAAAGAAG AGCTAGAAGA AGCCCGGGAC ATAGCTAGGC GGCGCTCTTT AGGGAATATC 
AAGTTTATTG GAGAGTTATT CAAGTTGAAG ATGTTAACAG AAGCAATAAT GCATGACTGT 
GTGGTCAAGC TACTTAAGAA CCATGATGAG GAGTCCCTGG AATGCCTCTG CCGCCTCCTC 
ACCACTATAG GCAAAGACCT GGACTTTGCA AAAGCCAAGC CTCGAATGGA TCAGTATTTC 
AACCAAATGG AAAAAATCAT TAAAGAAAAG AAGACCTCAT CTCGCATCCG TTTTATGCTG 
CAGGATGTAC TCGATCTGCG GCAGAGTAAT TGGGTGCCAC GCCGAGGGGA TCAGGGTCCT 
AAGACTATTG ATCAAATTCA CAAGGAGGCT GAGATGGAAG AGCACCGAGA ACATATTAAA 
GTACAGCAGC TCATGGCCAA GGGCAGCGAC AAACGTCGGG GTGGCCCTCC AGGCCCGCCT 
ATCAACCGTG GCCTTCCACT TGTAGATGAT GGTGGCTGGA ATACAGTCCC CATTAGCAAA 
GGCAGTCGCC CCATTGACAC CTCACGGCTC ACCAAGATCA CAAAGCCTGG TTCCATTGAT 
TCTAACAACC AGCTTTTTGC ACCTGGAGGA CGACTGAGTT GGGGCAAGGG CAGCAGTGGG 
GGCTCAGGAG CCAAGCCCTC AGACACAGCA TCGGAAGCTA CTCGTCCAGC TACCTTGAAT 
CGCTTTTCTG CTCTTCAACA AACATTACCC GCAGAGAACA CAGATAACAG ACGTGTTGTA 
CAGAGGAGTA GCTTGAGCCG GGAACGAGGT GAGAAAGCTG GGGACCGGGG AGACCGACTA 
GAGCGGAGTG AGCGGGGAGG TGACCGTGGG GACCGACTTG ATCGTGCCAG AACACCTGCC 
ACCAAGCGAA GTTTTAGCAA AGAAGTGGAG GAGCGAAGTA GAGAGCGGCC ATCCCAGCCT 
GAGGGACTCC GCAAGGCAGC TAGCCTCACA GAGGATCGTG GTCGGGATCC TGTGAAGCGG 
GAAGCCACTC TACCTCCAGT GAGCCCTCCA AAGGCTGCGC TGTCTGTGGA TGAGGTGGAG 
AAGAAATCTA AGGCCATCAT TGAGGAATAT CTCCATCTCA ATGACATGAA GGAGGCAGTA 
CAGTGTGTCC AGGAGCTGGC TTCACCCTCC TTGCTCTTCA TCTTCGTACG GCTTGGTATC 
GAGTCCACGC TGGAGCGTAG TACCATTGCT CGTGAGCATA TGGGGCGACT ACTGCACCAA 
CTGCTCTGTG CGGGGCACCT CTCTACTGCC CAGTACTATC AAGGGCTGTA TGAAACACTA 
GAATTGGCTG AGGACATGGA AATTGATATC CCTCATGTAT GGCTTTACCT GGCAGAACTG 
ATTACACCTA TTCTCCAGGA AGATGGGGTG CCCATGGGAG AGCTCTTCAG GGAAATTACG 
AAGCCTCTGA GACCCATGGG CAAAGCTACT TCTTTATTGC TGGAGATCCT GGGTCTCTTA 
TGCAAGAGCA TGGGTCCCAA AAAGGTGGGG ATGCTGTGGC GAGAGGCTGG GCTGAGCTGG 
AGGGAATTTC TAGCAGAGGG CCAAGACGTT GGCTCATTTG TGGCTGAAAA GAAGGTGGAA 
TATACCTTGG GAGAAGAATC TGAAGCTCCT GGCCAGAGGA CACTTGCCTT TGAGGAGCTT 
CGTAGGCAGC TAGAGAAGCT GCTGAAGGAC GGCGGCAGTA ATCAGCGTGT GTTCGACTGG 
ATAGACGCCA ACCTAAATGA GCAGCAGATA GCATCCAATA CATTAGTTCG AGCCCTCATG 
ACAACTGTCT GTTATTCTGC AATTATCTTT GAGACTCCTC TCCGAGTGGA TGTTCAAGTG 
TTGAAAGTGC GAGCAAGACT GCTGCAGAAA TACCTGTGTG ATGAGCAGAA GGAGCTACAA 
GCACTCTATG CTCTCCAGGC CCTTGTAGTG ACCTTAGAAC AGCCTGCCAA CCTGCTTCGG 
ATGTTCTTTG ATGCTCTATA TGATGAGGAC GTGGTGAAGG AAGACGCCTT CTACAGCTGG 
GAGAGCAGCA AGGATCCTGC TGAACAGCAG GGCAAGGGTG TGGCCCTTAA ATCTGTCACA 
GCATTCTTCA ATTGGCTTCG TGAGGCTGAG GACGAGGAGT CTGATCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104887.1
CDS	272..4765
Translation	MMIPSQISYSASQGAYYIPGQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_030249027.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249027.1

ORF Insert Sequence:

ATGATGATCC CTTCCCAGAT CTCCTACTCA GCCTCCCAAG GAGCCTACTA TATCCCTGGA 
CAGCAGTACC CTGTGCAGCC AGGAGCCCCA GGCTTCTATC CAGGTGCAAG CCCTACCGAG 
TTTGGGACCT ATGCTGGAGC CTATTACCCA GCCCAAGGTG TGCAGCAGTT TCCTGCTAGT 
GTGGCTCCTG CCCCAGTTTT GATGAACCAG CCACCCCAGA TTGCTCCTAA GAGGGAACGG 
AAAACTATCC GAATTCGAGA CCCAAACCAA GGAGGGAAGG ATATCACAGA AGAGATCATG 
TCTGGGGCCC GCACTGCCTC CACACCCACT CCTCCCCAGA CGGGAGGCAG TCTGGAGCCT 
CAACCCAATG GGGAGTCGCC TCAGGTTGCT GTCATTATCC GGCCAGATGA CCGGTCGCAG 
GGAGCAGCCA TTGGGGGGCG GCCAGGACTG CCTGGCCCAG AGCATAGCCC TGGCACAGAA 
TCTCAGCCTT CGTCGCCTTC TCCAACCCCA TCACCACCCC CAATTTTGGA GCCGGGGTCT 
GAGTCTAATC TTGGAGTCCT CTCTATTCCT GGGGACACTA TGACAACAGG GATGATACCA 
ATGTCTGTAG AAGAATCGAC CCCTATCTCT TGTGAAACTG GGGAGCCGTA TTGCCTCTCT 
CCAGAACCCA CTCTTGCCGA ACCCATACTG GAAGTAGAAG TGACACTCAG CAAACCCATT 
CCAGAATCTG AGTTCTCTTC CAGTCCTCTC CAGGTTTCCA CGGCCCTGGT GCCTCACAAG 
GTTGAAACTC ATGAGCCCAA TGGCGTGATC CCATCTGAGG ATCTGGAACC AGAGGTGGAG 
TCAAGCACAG AGCCAGCTCC TCCCCCTCTA TCACCTTGTG CTTCTGAATC GCTCGTGCCC 
ATTGCTCCAA CTGCCCAGCC TGAGGAACTG CTCAACGGAG CCCCCTCACC ACCAGCTGTG 
GATTTAAGCC CAGTCAGTGA GCCAGAGGAA CAGGCCAAGA AGGTTTCATC AGCAGCACTG 
GCCAGCATTC TCTCTCCTGC TCCACCTGTG GCTCCTTCAG ATACTTCTCC TGCTCAGGAG 
GAAGAAATGG AAGAAGATGA CGATGACGAA GAAGGTGGAG AAGCTGAGAG TGAGAAGGGA 
GGAGAGGACG TCCCCCTTGA CAGTACTCCT GTCCCAGCCC AGCTGTCTCA GAATTTAGAG 
GTGGCAGCAG CTACCCAGGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAAGAG 
CTAAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGT GGACCCAGCA 
GTACCAGAGG TAGAGAATCA GCCTCCCACT GGCAGCAACC CAAGCCCAGA GTCTGAGGGC 
AGCATGGTGC CCACACAGCC TGAGGAAACA GAAGAAACCT GGGACTCTAA GGAAGACAAA 
ATTCATAATG CTGAGAACAT CCAGCCTGGG GAGCAGAAGT ATGAGTACAA GTCAGATCAG 
TGGAAGCCTC TAAACCTTGA AGAAAAGAAG CGTTATGACA GAGAATTCCT GCTGGGCTTT 
CAGTTCATCT TTGCCAGCAT GCAGAAGCCA GAAGGATTGC CCCATATCAC TGATGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTTCGG CAACTGGATC CCTCCAGACT ACCTGGCATA 
AACTGTGGCC CAGATTTCAC TCCATCTTTT GCCAATCTTG GCCGACCAGC CCTCAGCAAC 
CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG 
GGACCCAGGC GCTCTCAGCA GGGCCCACGA AAGGAAACTC GCAAGATCAT TTCCTCAGTG 
ATAATGACTG AAGACATAAA ACTAAATAAA GCAGAGAAGG CTTGGAAACC CAGTAGCAAA 
CGGACAGCAG CTGATAAGGA TCGAGGGGAA GAGGATGCTG ATGGAAGCAA GACCCAGGAC 
CTGTTCCGCA GGGTACGCTC CATCCTGAAT AAGCTGACAC CCCAGATGTT TCAGCAGCTG 
ATGAAGCAGG TGACACAGCT GGCCATTGAC ACGGAGGAAC GCCTCAAAGG AGTTATTGAC 
CTCATCTTTG AGAAAGCCAT TTCAGAGCCC AACTTCTCTG TGGCTTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTAAC TGTGAATTTT 
CGAAAACTGT TGTTAAATCG GTGCCAGAAG GAATTTGAGA AAGACAAAGA TGATGATGAA 
GTTTTTGAAA AAAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAAGA ACGGGGACGC 
CTGAAAGAAG AGCTAGAAGA AGCCCGGGAC ATAGCTAGGC GGCGCTCTTT AGGGAATATC 
AAGTTTATTG GAGAGTTATT CAAGTTGAAG ATGTTAACAG AAGCAATAAT GCATGACTGT 
GTGGTCAAGC TACTTAAGAA CCATGATGAG GAGTCCCTGG AATGCCTCTG CCGCCTCCTC 
ACCACTATAG GCAAAGACCT GGACTTTGCA AAAGCCAAGC CTCGAATGGA TCAGTATTTC 
AACCAAATGG AAAAAATCAT TAAAGAAAAG AAGACCTCAT CTCGCATCCG TTTTATGCTG 
CAGGATGTAC TCGATCTGCG GCAGAGTAAT TGGGTGCCAC GCCGAGGGGA TCAGGGTCCT 
AAGACTATTG ATCAAATTCA CAAGGAGGCT GAGATGGAAG AGCACCGAGA ACATATTAAA 
GTACAGCAGC TCATGGCCAA GGGCAGCGAC AAACGTCGGG GTGGCCCTCC AGGCCCGCCT 
ATCAACCGTG GCCTTCCACT TGTAGATGAT GGTGGCTGGA ATACAGTCCC CATTAGCAAA 
GGCAGTCGCC CCATTGACAC CTCACGGCTC ACCAAGATCA CAAAGCCTGG TTCCATTGAT 
TCTAACAACC AGCTTTTTGC ACCTGGAGGA CGACTGAGTT GGGGCAAGGG CAGCAGTGGG 
GGCTCAGGAG CCAAGCCCTC AGACACAGCA TCGGAAGCTA CTCGTCCAGC TACCTTGAAT 
CGCTTTTCTG CTCTTCAACA AACATTACCC GCAGAGAACA CAGATAACAG ACGTGTTGTA 
CAGAGGAGTA GCTTGAGCCG GGAACGAGGT GAGAAAGCTG GGGACCGGGG AGACCGACTA 
GAGCGGAGTG AGCGGGGAGG TGACCGTGGG GACCGACTTG ATCGTGCCAG AACACCTGCC 
ACCAAGCGAA GTTTTAGCAA AGAAGTGGAG GAGCGAAGTA GAGAGCGGCC ATCCCAGCCT 
GAGGGACTCC GCAAGGCAGC TAGCCTCACA GAGGATCGTG GTCGGGATCC TGTGAAGCGG 
GAAGCCACTC TACCTCCAGT GAGCCCTCCA AAGGCTGCGC TGTCTGTGGA TGAGGTGGAG 
AAGAAATCTA AGGCCATCAT TGAGGAATAT CTCCATCTCA ATGACATGAA GGAGGCAGTA 
CAGTGTGTCC AGGAGCTGGC TTCACCCTCC TTGCTCTTCA TCTTCGTACG GCTTGGTATC 
GAGTCCACGC TGGAGCGTAG TACCATTGCT CGTGAGCATA TGGGGCGACT ACTGCACCAA 
CTGCTCTGTG CGGGGCACCT CTCTACTGCC CAGTACTATC AAGGGCTGTA TGAAACACTA 
GAATTGGCTG AGGACATGGA AATTGATATC CCTCATGTAT GGCTTTACCT GGCAGAACTG 
ATTACACCTA TTCTCCAGGA AGATGGGGTG CCCATGGGAG AGCTCTTCAG GGAAATTACG 
AAGCCTCTGA GACCCATGGG CAAAGCTACT TCTTTATTGC TGGAGATCCT GGGTCTCTTA 
TGCAAGAGCA TGGGTCCCAA AAAGGTGGGG ATGCTGTGGC GAGAGGCTGG GCTGAGCTGG 
AGGGAATTTC TAGCAGAGGG CCAAGACGTT GGCTCATTTG TGGCTGAAAA GAAGGTGGAA 
TATACCTTGG GAGAAGAATC TGAAGCTCCT GGCCAGAGGA CACTTGCCTT TGAGGAGCTT 
CGTAGGCAGC TAGAGAAGCT GCTGAAGGAC GGCGGCAGTA ATCAGCGTGT GTTCGACTGG 
ATAGACGCCA ACCTAAATGA GCAGCAGATA GCATCCAATA CATTAGTTCG AGCCCTCATG 
ACAACTGTCT GTTATTCTGC AATTATCTTT GAGACTCCTC TCCGAGTGGA TGTTCAAGTG 
TTGAAAGTGC GAGCAAGACT GCTGCAGAAA TACCTGTGTG ATGAGCAGAA GGAGCTACAA 
GCACTCTATG CTCTCCAGGC CCTTGTAGTG ACCTTAGAAC AGCCTGCCAA CCTGCTTCGG 
ATGTTCTTTG ATGCTCTATA TGATGAGGAC GTGGTGAAGG AAGACGCCTT CTACAGCTGG 
GAGAGCAGCA AGGATCCTGC TGAACAGCAG GGCAAGGGTG TGGCCCTTAA ATCTGTCACA 
GCATTCTTCA ATTGGCTTCG TGAGGCTGAG GACGAGGAGT CTGATCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( NM_001304432.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001291361.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu72647
Clone ID Related Accession (Same CDS sequence)	NM_001304432.1
Accession Version	NM_001304432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY789918.1, BC066038.1 and BC066103.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform c), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC 
TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA 
GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT 
GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG 
GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG 
ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG 
AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG 
GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG 
TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA 
AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG 
CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA 
GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA 
CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC 
AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA 
AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC 
GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT 
CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC 
ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAATGATGGT 
GGCTGGAATA CAGTCCCCAT TAGCAAAGGC AGTCGCCCCA TTGACACCTC ACGGCTCACC 
AAGATCACAA AGCCTGGTTC CATTGATTCT AACAACCAGC TTTTTGCACC TGGAGGACGA 
CTGAGTTGGG GCAAGGGCAG CAGTGGGGGC TCAGGAGCCA AGCCCTCAGA CACAGCATCG 
GAAGCTACTC GTCCAGCTAC CTTGAATCGC TTTTCTGCTC TTCAACAAAC ATTACCCGCA 
GAGAACACAG ATAACAGACG TGTTGTACAG AGGAGTAGCT TGAGCCGGGA ACGAGGTGAG 
AAAGCTGGGG ACCGGGGAGA CCGACTAGAG CGGAGTGAGC GGGGAGGTGA CCGTGGGGAC 
CGACTTGATC GTGCCAGAAC ACCTGCCACC AAGCGAAGTT TTAGCAAAGA AGTGGAGGAG 
CGAAGTAGAG AGCGGCCATC CCAGCCTGAG GGACTCCGCA AGGCAGCTAG CCTCACAGAG 
GATCGTGGTC GGGATCCTGT GAAGCGGGAA GCCACTCTAC CTCCAGTGAG CCCTCCAAAG 
GCTGCGCTGT CTGTGGATGA GGTGGAGAAG AAATCTAAGG CCATCATTGA GGAATATCTC 
CATCTCAATG ACATGAAGGA GGCAGTACAG TGTGTCCAGG AGCTGGCTTC ACCCTCCTTG 
CTCTTCATCT TCGTACGGCT TGGTATCGAG TCCACGCTGG AGCGTAGTAC CATTGCTCGT 
GAGCATATGG GGCGACTACT GCACCAACTG CTCTGTGCGG GGCACCTCTC TACTGCCCAG 
TACTATCAAG GGCTGTATGA AACACTAGAA TTGGCTGAGG ACATGGAAAT TGATATCCCT 
CATGTATGGC TTTACCTGGC AGAACTGATT ACACCTATTC TCCAGGAAGA TGGGGTGCCC 
ATGGGAGAGC TCTTCAGGGA AATTACGAAG CCTCTGAGAC CCATGGGCAA AGCTACTTCT 
TTATTGCTGG AGATCCTGGG TCTCTTATGC AAGAGCATGG GTCCCAAAAA GGTGGGGATG 
CTGTGGCGAG AGGCTGGGCT GAGCTGGAGG GAATTTCTAG CAGAGGGCCA AGACGTTGGC 
TCATTTGTGG CTGAAAAGAA GGTGGAATAT ACCTTGGGAG AAGAATCTGA AGCTCCTGGC 
CAGAGGACAC TTGCCTTTGA GGAGCTTCGT AGGCAGCTAG AGAAGCTGCT GAAGGACGGC 
GGCAGTAATC AGCGTGTGTT CGACTGGATA GACGCCAACC TAAATGAGCA GCAGATAGCA 
TCCAATACAT TAGTTCGAGC CCTCATGACA ACTGTCTGTT ATTCTGCAAT TATCTTTGAG 
ACTCCTCTCC GAGTGGATGT TCAAGTGTTG AAAGTGCGAG CAAGACTGCT GCAGAAATAC 
CTGTGTGATG AGCAGAAGGA GCTACAAGCA CTCTATGCTC TCCAGGCCCT TGTAGTGACC 
TTAGAACAGC CTGCCAACCT GCTTCGGATG TTCTTTGATG CTCTATATGA TGAGGACGTG 
GTGAAGGAAG ACGCCTTCTA CAGCTGGGAG AGCAGCAAGG ATCCTGCTGA ACAGCAGGGC 
AAGGGTGTGG CCCTTAAATC TGTCACAGCA TTCTTCAATT GGCTTCGTGA GGCTGAGGAC 
GAGGAGTCTG ATCACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291361.1
CDS	185..4945
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	NM_001304432.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304432.1

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC 
TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA 
GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT 
GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG 
GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG 
ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG 
AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG 
GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG 
TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA 
AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG 
CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA 
GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA 
CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC 
AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA 
AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC 
GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT 
CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC 
ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAATGATGGT 
GGCTGGAATA CAGTCCCCAT TAGCAAAGGC AGTCGCCCCA TTGACACCTC ACGGCTCACC 
AAGATCACAA AGCCTGGTTC CATTGATTCT AACAACCAGC TTTTTGCACC TGGAGGACGA 
CTGAGTTGGG GCAAGGGCAG CAGTGGGGGC TCAGGAGCCA AGCCCTCAGA CACAGCATCG 
GAAGCTACTC GTCCAGCTAC CTTGAATCGC TTTTCTGCTC TTCAACAAAC ATTACCCGCA 
GAGAACACAG ATAACAGACG TGTTGTACAG AGGAGTAGCT TGAGCCGGGA ACGAGGTGAG 
AAAGCTGGGG ACCGGGGAGA CCGACTAGAG CGGAGTGAGC GGGGAGGTGA CCGTGGGGAC 
CGACTTGATC GTGCCAGAAC ACCTGCCACC AAGCGAAGTT TTAGCAAAGA AGTGGAGGAG 
CGAAGTAGAG AGCGGCCATC CCAGCCTGAG GGACTCCGCA AGGCAGCTAG CCTCACAGAG 
GATCGTGGTC GGGATCCTGT GAAGCGGGAA GCCACTCTAC CTCCAGTGAG CCCTCCAAAG 
GCTGCGCTGT CTGTGGATGA GGTGGAGAAG AAATCTAAGG CCATCATTGA GGAATATCTC 
CATCTCAATG ACATGAAGGA GGCAGTACAG TGTGTCCAGG AGCTGGCTTC ACCCTCCTTG 
CTCTTCATCT TCGTACGGCT TGGTATCGAG TCCACGCTGG AGCGTAGTAC CATTGCTCGT 
GAGCATATGG GGCGACTACT GCACCAACTG CTCTGTGCGG GGCACCTCTC TACTGCCCAG 
TACTATCAAG GGCTGTATGA AACACTAGAA TTGGCTGAGG ACATGGAAAT TGATATCCCT 
CATGTATGGC TTTACCTGGC AGAACTGATT ACACCTATTC TCCAGGAAGA TGGGGTGCCC 
ATGGGAGAGC TCTTCAGGGA AATTACGAAG CCTCTGAGAC CCATGGGCAA AGCTACTTCT 
TTATTGCTGG AGATCCTGGG TCTCTTATGC AAGAGCATGG GTCCCAAAAA GGTGGGGATG 
CTGTGGCGAG AGGCTGGGCT GAGCTGGAGG GAATTTCTAG CAGAGGGCCA AGACGTTGGC 
TCATTTGTGG CTGAAAAGAA GGTGGAATAT ACCTTGGGAG AAGAATCTGA AGCTCCTGGC 
CAGAGGACAC TTGCCTTTGA GGAGCTTCGT AGGCAGCTAG AGAAGCTGCT GAAGGACGGC 
GGCAGTAATC AGCGTGTGTT CGACTGGATA GACGCCAACC TAAATGAGCA GCAGATAGCA 
TCCAATACAT TAGTTCGAGC CCTCATGACA ACTGTCTGTT ATTCTGCAAT TATCTTTGAG 
ACTCCTCTCC GAGTGGATGT TCAAGTGTTG AAAGTGCGAG CAAGACTGCT GCAGAAATAC 
CTGTGTGATG AGCAGAAGGA GCTACAAGCA CTCTATGCTC TCCAGGCCCT TGTAGTGACC 
TTAGAACAGC CTGCCAACCT GCTTCGGATG TTCTTTGATG CTCTATATGA TGAGGACGTG 
GTGAAGGAAG ACGCCTTCTA CAGCTGGGAG AGCAGCAAGG ATCCTGCTGA ACAGCAGGGC 
AAGGGTGTGG CCCTTAAATC TGTCACAGCA TTCTTCAATT GGCTTCGTGA GGCTGAGGAC 
GAGGAGTCTG ATCACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( NM_145941.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666053.2 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1, mRNA.

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CloneID	OMu14490
Clone ID Related Accession (Same CDS sequence)	XM_006521937.1 , NM_145941.3
Accession Version	NM_145941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY789918.1 and BC066103.1. On Jan 27, 2015 this sequence version replaced NM_145941.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849379, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
GCTGGCCTGG GACCCAGGCG CTCTCAGCAG GGCCCACGAA AGGAAACTCG CAAGATCATT 
TCCTCAGTGA TAATGACTGA AGACATAAAA CTAAATAAAG CAGAGAAGGC TTGGAAACCC 
AGTAGCAAAC GGACAGCAGC TGATAAGGAT CGAGGGGAAG AGGATGCTGA TGGAAGCAAG 
ACCCAGGACC TGTTCCGCAG GGTACGCTCC ATCCTGAATA AGCTGACACC CCAGATGTTT 
CAGCAGCTGA TGAAGCAGGT GACACAGCTG GCCATTGACA CGGAGGAACG CCTCAAAGGA 
GTTATTGACC TCATCTTTGA GAAAGCCATT TCAGAGCCCA ACTTCTCTGT GGCTTATGCC 
AACATGTGCC GCTGCCTCAT GGCGCTGAAA GTGCCCACTA CAGAAAAGCC AACAGTAACT 
GTGAATTTTC GAAAACTGTT GTTAAATCGG TGCCAGAAGG AATTTGAGAA AGACAAAGAT 
GATGATGAAG TTTTTGAAAA AAAGCAAAAA GAGATGGATG AAGCTGCTAC GGCAGAAGAA 
CGGGGACGCC TGAAAGAAGA GCTAGAAGAA GCCCGGGACA TAGCTAGGCG GCGCTCTTTA 
GGGAATATCA AGTTTATTGG AGAGTTATTC AAGTTGAAGA TGTTAACAGA AGCAATAATG 
CATGACTGTG TGGTCAAGCT ACTTAAGAAC CATGATGAGG AGTCCCTGGA ATGCCTCTGC 
CGCCTCCTCA CCACTATAGG CAAAGACCTG GACTTTGCAA AAGCCAAGCC TCGAATGGAT 
CAGTATTTCA ACCAAATGGA AAAAATCATT AAAGAAAAGA AGACCTCATC TCGCATCCGT 
TTTATGCTGC AGGATGTACT CGATCTGCGG CAGAGTAATT GGGTGCCACG CCGAGGGGAT 
CAGGGTCCTA AGACTATTGA TCAAATTCAC AAGGAGGCTG AGATGGAAGA GCACCGAGAA 
CATATTAAAG TACAGCAGCT CATGGCCAAG GGCAGCGACA AACGTCGGGG TGGCCCTCCA 
GGCCCGCCTA TCAACCGTGG CCTTCCACTT GTAGATGATG GTGGCTGGAA TACAGTCCCC 
ATTAGCAAAG GCAGTCGCCC CATTGACACC TCACGGCTCA CCAAGATCAC AAAGCCTGGT 
TCCATTGATT CTAACAACCA GCTTTTTGCA CCTGGAGGAC GACTGAGTTG GGGCAAGGGC 
AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA GACACAGCAT CGGAAGCTAC TCGTCCAGCT 
ACCTTGAATC GCTTTTCTGC TCTTCAACAA ACATTACCCG CAGAGAACAC AGATAACAGA 
CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGACTAG AGCGGAGTGA GCGGGGAGGT GACCGTGGGG ACCGACTTGA TCGTGCCAGA 
ACACCTGCCA CCAAGCGAAG TTTTAGCAAA GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA 
TCCCAGCCTG AGGGACTCCG CAAGGCAGCT AGCCTCACAG AGGATCGTGG TCGGGATCCT 
GTGAAGCGGG AAGCCACTCT ACCTCCAGTG AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT 
GAGGTGGAGA AGAAATCTAA GGCCATCATT GAGGAATATC TCCATCTCAA TGACATGAAG 
GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT TCACCCTCCT TGCTCTTCAT CTTCGTACGG 
CTTGGTATCG AGTCCACGCT GGAGCGTAGT ACCATTGCTC GTGAGCATAT GGGGCGACTA 
CTGCACCAAC TGCTCTGTGC GGGGCACCTC TCTACTGCCC AGTACTATCA AGGGCTGTAT 
GAAACACTAG AATTGGCTGA GGACATGGAA ATTGATATCC CTCATGTATG GCTTTACCTG 
GCAGAACTGA TTACACCTAT TCTCCAGGAA GATGGGGTGC CCATGGGAGA GCTCTTCAGG 
GAAATTACGA AGCCTCTGAG ACCCATGGGC AAAGCTACTT CTTTATTGCT GGAGATCCTG 
GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG 
CTGAGCTGGA GGGAATTTCT AGCAGAGGGC CAAGACGTTG GCTCATTTGT GGCTGAAAAG 
AAGGTGGAAT ATACCTTGGG AGAAGAATCT GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT 
GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG CTGAAGGACG GCGGCAGTAA TCAGCGTGTG 
TTCGACTGGA TAGACGCCAA CCTAAATGAG CAGCAGATAG CATCCAATAC ATTAGTTCGA 
GCCCTCATGA CAACTGTCTG TTATTCTGCA ATTATCTTTG AGACTCCTCT CCGAGTGGAT 
GTTCAAGTGT TGAAAGTGCG AGCAAGACTG CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG 
GAGCTACAAG CACTCTATGC TCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTGCCAAC 
CTGCTTCGGA TGTTCTTTGA TGCTCTATAT GATGAGGACG TGGTGAAGGA AGACGCCTTC 
TACAGCTGGG AGAGCAGCAA GGATCCTGCT GAACAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CATTCTTCAA TTGGCTTCGT GAGGCTGAGG ACGAGGAGTC TGATCACAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666053.2
CDS	185..4987
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	NM_145941.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145941.3

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
GCTGGCCTGG GACCCAGGCG CTCTCAGCAG GGCCCACGAA AGGAAACTCG CAAGATCATT 
TCCTCAGTGA TAATGACTGA AGACATAAAA CTAAATAAAG CAGAGAAGGC TTGGAAACCC 
AGTAGCAAAC GGACAGCAGC TGATAAGGAT CGAGGGGAAG AGGATGCTGA TGGAAGCAAG 
ACCCAGGACC TGTTCCGCAG GGTACGCTCC ATCCTGAATA AGCTGACACC CCAGATGTTT 
CAGCAGCTGA TGAAGCAGGT GACACAGCTG GCCATTGACA CGGAGGAACG CCTCAAAGGA 
GTTATTGACC TCATCTTTGA GAAAGCCATT TCAGAGCCCA ACTTCTCTGT GGCTTATGCC 
AACATGTGCC GCTGCCTCAT GGCGCTGAAA GTGCCCACTA CAGAAAAGCC AACAGTAACT 
GTGAATTTTC GAAAACTGTT GTTAAATCGG TGCCAGAAGG AATTTGAGAA AGACAAAGAT 
GATGATGAAG TTTTTGAAAA AAAGCAAAAA GAGATGGATG AAGCTGCTAC GGCAGAAGAA 
CGGGGACGCC TGAAAGAAGA GCTAGAAGAA GCCCGGGACA TAGCTAGGCG GCGCTCTTTA 
GGGAATATCA AGTTTATTGG AGAGTTATTC AAGTTGAAGA TGTTAACAGA AGCAATAATG 
CATGACTGTG TGGTCAAGCT ACTTAAGAAC CATGATGAGG AGTCCCTGGA ATGCCTCTGC 
CGCCTCCTCA CCACTATAGG CAAAGACCTG GACTTTGCAA AAGCCAAGCC TCGAATGGAT 
CAGTATTTCA ACCAAATGGA AAAAATCATT AAAGAAAAGA AGACCTCATC TCGCATCCGT 
TTTATGCTGC AGGATGTACT CGATCTGCGG CAGAGTAATT GGGTGCCACG CCGAGGGGAT 
CAGGGTCCTA AGACTATTGA TCAAATTCAC AAGGAGGCTG AGATGGAAGA GCACCGAGAA 
CATATTAAAG TACAGCAGCT CATGGCCAAG GGCAGCGACA AACGTCGGGG TGGCCCTCCA 
GGCCCGCCTA TCAACCGTGG CCTTCCACTT GTAGATGATG GTGGCTGGAA TACAGTCCCC 
ATTAGCAAAG GCAGTCGCCC CATTGACACC TCACGGCTCA CCAAGATCAC AAAGCCTGGT 
TCCATTGATT CTAACAACCA GCTTTTTGCA CCTGGAGGAC GACTGAGTTG GGGCAAGGGC 
AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA GACACAGCAT CGGAAGCTAC TCGTCCAGCT 
ACCTTGAATC GCTTTTCTGC TCTTCAACAA ACATTACCCG CAGAGAACAC AGATAACAGA 
CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGACTAG AGCGGAGTGA GCGGGGAGGT GACCGTGGGG ACCGACTTGA TCGTGCCAGA 
ACACCTGCCA CCAAGCGAAG TTTTAGCAAA GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA 
TCCCAGCCTG AGGGACTCCG CAAGGCAGCT AGCCTCACAG AGGATCGTGG TCGGGATCCT 
GTGAAGCGGG AAGCCACTCT ACCTCCAGTG AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT 
GAGGTGGAGA AGAAATCTAA GGCCATCATT GAGGAATATC TCCATCTCAA TGACATGAAG 
GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT TCACCCTCCT TGCTCTTCAT CTTCGTACGG 
CTTGGTATCG AGTCCACGCT GGAGCGTAGT ACCATTGCTC GTGAGCATAT GGGGCGACTA 
CTGCACCAAC TGCTCTGTGC GGGGCACCTC TCTACTGCCC AGTACTATCA AGGGCTGTAT 
GAAACACTAG AATTGGCTGA GGACATGGAA ATTGATATCC CTCATGTATG GCTTTACCTG 
GCAGAACTGA TTACACCTAT TCTCCAGGAA GATGGGGTGC CCATGGGAGA GCTCTTCAGG 
GAAATTACGA AGCCTCTGAG ACCCATGGGC AAAGCTACTT CTTTATTGCT GGAGATCCTG 
GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG 
CTGAGCTGGA GGGAATTTCT AGCAGAGGGC CAAGACGTTG GCTCATTTGT GGCTGAAAAG 
AAGGTGGAAT ATACCTTGGG AGAAGAATCT GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT 
GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG CTGAAGGACG GCGGCAGTAA TCAGCGTGTG 
TTCGACTGGA TAGACGCCAA CCTAAATGAG CAGCAGATAG CATCCAATAC ATTAGTTCGA 
GCCCTCATGA CAACTGTCTG TTATTCTGCA ATTATCTTTG AGACTCCTCT CCGAGTGGAT 
GTTCAAGTGT TGAAAGTGCG AGCAAGACTG CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG 
GAGCTACAAG CACTCTATGC TCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTGCCAAC 
CTGCTTCGGA TGTTCTTTGA TGCTCTATAT GATGAGGACG TGGTGAAGGA AGACGCCTTC 
TACAGCTGGG AGAGCAGCAA GGATCCTGCT GAACAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CATTCTTCAA TTGGCTTCGT GAGGCTGAGG ACGAGGAGTC TGATCACAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521937.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522000.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X3, mRNA.

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CloneID	OMu14490
Clone ID Related Accession (Same CDS sequence)	XM_006521937.1 , NM_145941.3
Accession Version	XM_006521937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
GCTGGCCTGG GACCCAGGCG CTCTCAGCAG GGCCCACGAA AGGAAACTCG CAAGATCATT 
TCCTCAGTGA TAATGACTGA AGACATAAAA CTAAATAAAG CAGAGAAGGC TTGGAAACCC 
AGTAGCAAAC GGACAGCAGC TGATAAGGAT CGAGGGGAAG AGGATGCTGA TGGAAGCAAG 
ACCCAGGACC TGTTCCGCAG GGTACGCTCC ATCCTGAATA AGCTGACACC CCAGATGTTT 
CAGCAGCTGA TGAAGCAGGT GACACAGCTG GCCATTGACA CGGAGGAACG CCTCAAAGGA 
GTTATTGACC TCATCTTTGA GAAAGCCATT TCAGAGCCCA ACTTCTCTGT GGCTTATGCC 
AACATGTGCC GCTGCCTCAT GGCGCTGAAA GTGCCCACTA CAGAAAAGCC AACAGTAACT 
GTGAATTTTC GAAAACTGTT GTTAAATCGG TGCCAGAAGG AATTTGAGAA AGACAAAGAT 
GATGATGAAG TTTTTGAAAA AAAGCAAAAA GAGATGGATG AAGCTGCTAC GGCAGAAGAA 
CGGGGACGCC TGAAAGAAGA GCTAGAAGAA GCCCGGGACA TAGCTAGGCG GCGCTCTTTA 
GGGAATATCA AGTTTATTGG AGAGTTATTC AAGTTGAAGA TGTTAACAGA AGCAATAATG 
CATGACTGTG TGGTCAAGCT ACTTAAGAAC CATGATGAGG AGTCCCTGGA ATGCCTCTGC 
CGCCTCCTCA CCACTATAGG CAAAGACCTG GACTTTGCAA AAGCCAAGCC TCGAATGGAT 
CAGTATTTCA ACCAAATGGA AAAAATCATT AAAGAAAAGA AGACCTCATC TCGCATCCGT 
TTTATGCTGC AGGATGTACT CGATCTGCGG CAGAGTAATT GGGTGCCACG CCGAGGGGAT 
CAGGGTCCTA AGACTATTGA TCAAATTCAC AAGGAGGCTG AGATGGAAGA GCACCGAGAA 
CATATTAAAG TACAGCAGCT CATGGCCAAG GGCAGCGACA AACGTCGGGG TGGCCCTCCA 
GGCCCGCCTA TCAACCGTGG CCTTCCACTT GTAGATGATG GTGGCTGGAA TACAGTCCCC 
ATTAGCAAAG GCAGTCGCCC CATTGACACC TCACGGCTCA CCAAGATCAC AAAGCCTGGT 
TCCATTGATT CTAACAACCA GCTTTTTGCA CCTGGAGGAC GACTGAGTTG GGGCAAGGGC 
AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA GACACAGCAT CGGAAGCTAC TCGTCCAGCT 
ACCTTGAATC GCTTTTCTGC TCTTCAACAA ACATTACCCG CAGAGAACAC AGATAACAGA 
CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGACTAG AGCGGAGTGA GCGGGGAGGT GACCGTGGGG ACCGACTTGA TCGTGCCAGA 
ACACCTGCCA CCAAGCGAAG TTTTAGCAAA GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA 
TCCCAGCCTG AGGGACTCCG CAAGGCAGCT AGCCTCACAG AGGATCGTGG TCGGGATCCT 
GTGAAGCGGG AAGCCACTCT ACCTCCAGTG AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT 
GAGGTGGAGA AGAAATCTAA GGCCATCATT GAGGAATATC TCCATCTCAA TGACATGAAG 
GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT TCACCCTCCT TGCTCTTCAT CTTCGTACGG 
CTTGGTATCG AGTCCACGCT GGAGCGTAGT ACCATTGCTC GTGAGCATAT GGGGCGACTA 
CTGCACCAAC TGCTCTGTGC GGGGCACCTC TCTACTGCCC AGTACTATCA AGGGCTGTAT 
GAAACACTAG AATTGGCTGA GGACATGGAA ATTGATATCC CTCATGTATG GCTTTACCTG 
GCAGAACTGA TTACACCTAT TCTCCAGGAA GATGGGGTGC CCATGGGAGA GCTCTTCAGG 
GAAATTACGA AGCCTCTGAG ACCCATGGGC AAAGCTACTT CTTTATTGCT GGAGATCCTG 
GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG 
CTGAGCTGGA GGGAATTTCT AGCAGAGGGC CAAGACGTTG GCTCATTTGT GGCTGAAAAG 
AAGGTGGAAT ATACCTTGGG AGAAGAATCT GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT 
GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG CTGAAGGACG GCGGCAGTAA TCAGCGTGTG 
TTCGACTGGA TAGACGCCAA CCTAAATGAG CAGCAGATAG CATCCAATAC ATTAGTTCGA 
GCCCTCATGA CAACTGTCTG TTATTCTGCA ATTATCTTTG AGACTCCTCT CCGAGTGGAT 
GTTCAAGTGT TGAAAGTGCG AGCAAGACTG CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG 
GAGCTACAAG CACTCTATGC TCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTGCCAAC 
CTGCTTCGGA TGTTCTTTGA TGCTCTATAT GATGAGGACG TGGTGAAGGA AGACGCCTTC 
TACAGCTGGG AGAGCAGCAA GGATCCTGCT GAACAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CATTCTTCAA TTGGCTTCGT GAGGCTGAGG ACGAGGAGTC TGATCACAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522000.1
CDS	249..5051
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521937.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521937.1

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
GCTGGCCTGG GACCCAGGCG CTCTCAGCAG GGCCCACGAA AGGAAACTCG CAAGATCATT 
TCCTCAGTGA TAATGACTGA AGACATAAAA CTAAATAAAG CAGAGAAGGC TTGGAAACCC 
AGTAGCAAAC GGACAGCAGC TGATAAGGAT CGAGGGGAAG AGGATGCTGA TGGAAGCAAG 
ACCCAGGACC TGTTCCGCAG GGTACGCTCC ATCCTGAATA AGCTGACACC CCAGATGTTT 
CAGCAGCTGA TGAAGCAGGT GACACAGCTG GCCATTGACA CGGAGGAACG CCTCAAAGGA 
GTTATTGACC TCATCTTTGA GAAAGCCATT TCAGAGCCCA ACTTCTCTGT GGCTTATGCC 
AACATGTGCC GCTGCCTCAT GGCGCTGAAA GTGCCCACTA CAGAAAAGCC AACAGTAACT 
GTGAATTTTC GAAAACTGTT GTTAAATCGG TGCCAGAAGG AATTTGAGAA AGACAAAGAT 
GATGATGAAG TTTTTGAAAA AAAGCAAAAA GAGATGGATG AAGCTGCTAC GGCAGAAGAA 
CGGGGACGCC TGAAAGAAGA GCTAGAAGAA GCCCGGGACA TAGCTAGGCG GCGCTCTTTA 
GGGAATATCA AGTTTATTGG AGAGTTATTC AAGTTGAAGA TGTTAACAGA AGCAATAATG 
CATGACTGTG TGGTCAAGCT ACTTAAGAAC CATGATGAGG AGTCCCTGGA ATGCCTCTGC 
CGCCTCCTCA CCACTATAGG CAAAGACCTG GACTTTGCAA AAGCCAAGCC TCGAATGGAT 
CAGTATTTCA ACCAAATGGA AAAAATCATT AAAGAAAAGA AGACCTCATC TCGCATCCGT 
TTTATGCTGC AGGATGTACT CGATCTGCGG CAGAGTAATT GGGTGCCACG CCGAGGGGAT 
CAGGGTCCTA AGACTATTGA TCAAATTCAC AAGGAGGCTG AGATGGAAGA GCACCGAGAA 
CATATTAAAG TACAGCAGCT CATGGCCAAG GGCAGCGACA AACGTCGGGG TGGCCCTCCA 
GGCCCGCCTA TCAACCGTGG CCTTCCACTT GTAGATGATG GTGGCTGGAA TACAGTCCCC 
ATTAGCAAAG GCAGTCGCCC CATTGACACC TCACGGCTCA CCAAGATCAC AAAGCCTGGT 
TCCATTGATT CTAACAACCA GCTTTTTGCA CCTGGAGGAC GACTGAGTTG GGGCAAGGGC 
AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA GACACAGCAT CGGAAGCTAC TCGTCCAGCT 
ACCTTGAATC GCTTTTCTGC TCTTCAACAA ACATTACCCG CAGAGAACAC AGATAACAGA 
CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGACTAG AGCGGAGTGA GCGGGGAGGT GACCGTGGGG ACCGACTTGA TCGTGCCAGA 
ACACCTGCCA CCAAGCGAAG TTTTAGCAAA GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA 
TCCCAGCCTG AGGGACTCCG CAAGGCAGCT AGCCTCACAG AGGATCGTGG TCGGGATCCT 
GTGAAGCGGG AAGCCACTCT ACCTCCAGTG AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT 
GAGGTGGAGA AGAAATCTAA GGCCATCATT GAGGAATATC TCCATCTCAA TGACATGAAG 
GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT TCACCCTCCT TGCTCTTCAT CTTCGTACGG 
CTTGGTATCG AGTCCACGCT GGAGCGTAGT ACCATTGCTC GTGAGCATAT GGGGCGACTA 
CTGCACCAAC TGCTCTGTGC GGGGCACCTC TCTACTGCCC AGTACTATCA AGGGCTGTAT 
GAAACACTAG AATTGGCTGA GGACATGGAA ATTGATATCC CTCATGTATG GCTTTACCTG 
GCAGAACTGA TTACACCTAT TCTCCAGGAA GATGGGGTGC CCATGGGAGA GCTCTTCAGG 
GAAATTACGA AGCCTCTGAG ACCCATGGGC AAAGCTACTT CTTTATTGCT GGAGATCCTG 
GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG 
CTGAGCTGGA GGGAATTTCT AGCAGAGGGC CAAGACGTTG GCTCATTTGT GGCTGAAAAG 
AAGGTGGAAT ATACCTTGGG AGAAGAATCT GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT 
GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG CTGAAGGACG GCGGCAGTAA TCAGCGTGTG 
TTCGACTGGA TAGACGCCAA CCTAAATGAG CAGCAGATAG CATCCAATAC ATTAGTTCGA 
GCCCTCATGA CAACTGTCTG TTATTCTGCA ATTATCTTTG AGACTCCTCT CCGAGTGGAT 
GTTCAAGTGT TGAAAGTGCG AGCAAGACTG CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG 
GAGCTACAAG CACTCTATGC TCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTGCCAAC 
CTGCTTCGGA TGTTCTTTGA TGCTCTATAT GATGAGGACG TGGTGAAGGA AGACGCCTTC 
TACAGCTGGG AGAGCAGCAA GGATCCTGCT GAACAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CATTCTTCAA TTGGCTTCGT GAGGCTGAGG ACGAGGAGTC TGATCACAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521940.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522003.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA.

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CloneID	OMu14547
Clone ID Related Accession (Same CDS sequence)	XM_006521940.4 , XM_006521940.3
Accession Version	XM_006521940.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521940.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC 
TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA 
GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT 
GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG 
GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG 
ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG 
AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG 
GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG 
TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA 
AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG 
CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA 
GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA 
CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC 
AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA 
AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC 
GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT 
CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC 
ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAACCGTGGC 
CTTCCACTTG TAGATGATGG TGGCTGGAAT ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC 
ATTGACACCT CACGGCTCAC CAAGATCACA AAGCCTGGTT CCATTGATTC TAACAACCAG 
CTTTTTGCAC CTGGAGGACG ACTGAGTTGG GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC 
AAGCCCTCAG ACACAGCATC GGAAGCTACT CGTCCAGCTA CCTTGAATCG CTTTTCTGCT 
CTTCAACAAA CATTACCCGC AGAGAACACA GATAACAGAC GTGTTGTACA GAGGAGTAGC 
TTGAGCCGGG AACGAGGTGA GAAAGCTGGG GACCGGGGAG ACCGACTAGA GCGGAGTGAG 
CGGGGAGGTG ACCGTGGGGA CCGACTTGAT CGTGCCAGAA CACCTGCCAC CAAGCGAAGT 
TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA GAGCGGCCAT CCCAGCCTGA GGGACTCCGC 
AAGGCAGCTA GCCTCACAGA GGATCGTGGT CGGGATCCTG TGAAGCGGGA AGCCACTCTA 
CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG TCTGTGGATG AGGTGGAGAA GAAATCTAAG 
GCCATCATTG AGGAATATCT CCATCTCAAT GACATGAAGG AGGCAGTACA GTGTGTCCAG 
GAGCTGGCTT CACCCTCCTT GCTCTTCATC TTCGTACGGC TTGGTATCGA GTCCACGCTG 
GAGCGTAGTA CCATTGCTCG TGAGCATATG GGGCGACTAC TGCACCAACT GCTCTGTGCG 
GGGCACCTCT CTACTGCCCA GTACTATCAA GGGCTGTATG AAACACTAGA ATTGGCTGAG 
GACATGGAAA TTGATATCCC TCATGTATGG CTTTACCTGG CAGAACTGAT TACACCTATT 
CTCCAGGAAG ATGGGGTGCC CATGGGAGAG CTCTTCAGGG AAATTACGAA GCCTCTGAGA 
CCCATGGGCA AAGCTACTTC TTTATTGCTG GAGATCCTGG GTCTCTTATG CAAGAGCATG 
GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA GAGGCTGGGC TGAGCTGGAG GGAATTTCTA 
GCAGAGGGCC AAGACGTTGG CTCATTTGTG GCTGAAAAGA AGGTGGAATA TACCTTGGGA 
GAAGAATCTG AAGCTCCTGG CCAGAGGACA CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA 
GAGAAGCTGC TGAAGGACGG CGGCAGTAAT CAGCGTGTGT TCGACTGGAT AGACGCCAAC 
CTAAATGAGC AGCAGATAGC ATCCAATACA TTAGTTCGAG CCCTCATGAC AACTGTCTGT 
TATTCTGCAA TTATCTTTGA GACTCCTCTC CGAGTGGATG TTCAAGTGTT GAAAGTGCGA 
GCAAGACTGC TGCAGAAATA CCTGTGTGAT GAGCAGAAGG AGCTACAAGC ACTCTATGCT 
CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTGCCAACC TGCTTCGGAT GTTCTTTGAT 
GCTCTATATG ATGAGGACGT GGTGAAGGAA GACGCCTTCT ACAGCTGGGA GAGCAGCAAG 
GATCCTGCTG AACAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC ATTCTTCAAT 
TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT GATCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522003.1
CDS	230..5011
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521940.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521940.4

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC 
TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA 
GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT 
GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG 
GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG 
ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG 
AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG 
GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG 
TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA 
AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG 
CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA 
GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA 
CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC 
AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA 
AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC 
GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT 
CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC 
ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAACCGTGGC 
CTTCCACTTG TAGATGATGG TGGCTGGAAT ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC 
ATTGACACCT CACGGCTCAC CAAGATCACA AAGCCTGGTT CCATTGATTC TAACAACCAG 
CTTTTTGCAC CTGGAGGACG ACTGAGTTGG GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC 
AAGCCCTCAG ACACAGCATC GGAAGCTACT CGTCCAGCTA CCTTGAATCG CTTTTCTGCT 
CTTCAACAAA CATTACCCGC AGAGAACACA GATAACAGAC GTGTTGTACA GAGGAGTAGC 
TTGAGCCGGG AACGAGGTGA GAAAGCTGGG GACCGGGGAG ACCGACTAGA GCGGAGTGAG 
CGGGGAGGTG ACCGTGGGGA CCGACTTGAT CGTGCCAGAA CACCTGCCAC CAAGCGAAGT 
TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA GAGCGGCCAT CCCAGCCTGA GGGACTCCGC 
AAGGCAGCTA GCCTCACAGA GGATCGTGGT CGGGATCCTG TGAAGCGGGA AGCCACTCTA 
CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG TCTGTGGATG AGGTGGAGAA GAAATCTAAG 
GCCATCATTG AGGAATATCT CCATCTCAAT GACATGAAGG AGGCAGTACA GTGTGTCCAG 
GAGCTGGCTT CACCCTCCTT GCTCTTCATC TTCGTACGGC TTGGTATCGA GTCCACGCTG 
GAGCGTAGTA CCATTGCTCG TGAGCATATG GGGCGACTAC TGCACCAACT GCTCTGTGCG 
GGGCACCTCT CTACTGCCCA GTACTATCAA GGGCTGTATG AAACACTAGA ATTGGCTGAG 
GACATGGAAA TTGATATCCC TCATGTATGG CTTTACCTGG CAGAACTGAT TACACCTATT 
CTCCAGGAAG ATGGGGTGCC CATGGGAGAG CTCTTCAGGG AAATTACGAA GCCTCTGAGA 
CCCATGGGCA AAGCTACTTC TTTATTGCTG GAGATCCTGG GTCTCTTATG CAAGAGCATG 
GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA GAGGCTGGGC TGAGCTGGAG GGAATTTCTA 
GCAGAGGGCC AAGACGTTGG CTCATTTGTG GCTGAAAAGA AGGTGGAATA TACCTTGGGA 
GAAGAATCTG AAGCTCCTGG CCAGAGGACA CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA 
GAGAAGCTGC TGAAGGACGG CGGCAGTAAT CAGCGTGTGT TCGACTGGAT AGACGCCAAC 
CTAAATGAGC AGCAGATAGC ATCCAATACA TTAGTTCGAG CCCTCATGAC AACTGTCTGT 
TATTCTGCAA TTATCTTTGA GACTCCTCTC CGAGTGGATG TTCAAGTGTT GAAAGTGCGA 
GCAAGACTGC TGCAGAAATA CCTGTGTGAT GAGCAGAAGG AGCTACAAGC ACTCTATGCT 
CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTGCCAACC TGCTTCGGAT GTTCTTTGAT 
GCTCTATATG ATGAGGACGT GGTGAAGGAA GACGCCTTCT ACAGCTGGGA GAGCAGCAAG 
GATCCTGCTG AACAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC ATTCTTCAAT 
TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT GATCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521938.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522001.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA.

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CloneID	OMu51987
Clone ID Related Accession (Same CDS sequence)	XM_006521938.1 , XM_006521939.1 , XM_006521938.2
Accession Version	XM_006521938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522001.1
CDS	242..5026
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521938.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521938.2

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_030249025.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104885.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu51990
Clone ID Related Accession (Same CDS sequence)	XM_030249025.1 , XM_017316919.1 , XM_006521943.1
Accession Version	XM_030249025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104885.1
CDS	218..4903
Translation	MNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_030249025.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249025.1

ORF Insert Sequence:

ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_030249026.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104886.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu51991
Clone ID Related Accession (Same CDS sequence)	XM_006521944.3 , XM_030249026.1
Accession Version	XM_030249026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104886.1
CDS	218..4882
Translation	MNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_030249026.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249026.1

ORF Insert Sequence:

ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_030249023.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104883.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X8, mRNA.

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CloneID	OMu86905
Clone ID Related Accession (Same CDS sequence)	XM_030249023.1
Accession Version	XM_030249023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG 
CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA 
GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT 
TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA 
GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC 
TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG 
CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG 
CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT 
TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC 
CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG 
ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC 
GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT 
TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC 
AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT 
CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG 
CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT 
GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT 
GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT 
GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT 
GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG 
GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT 
GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT 
CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG 
CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC 
ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT 
GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC 
ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA 
ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC 
ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT 
GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG GCTGGCCTGG GACCCAGGCG CTCTCAGCAG 
GGCCCACGAA AGGAAACTCG CAAGATCATT TCCTCAGTGA TAATGACTGA AGACATAAAA 
CTAAATAAAG CAGAGAAGGC TTGGAAACCC AGTAGCAAAC GGACAGCAGC TGATAAGGAT 
CGAGGGGAAG AGGATGCTGA TGGAAGCAAG ACCCAGGACC TGTTCCGCAG GGTACGCTCC 
ATCCTGAATA AGCTGACACC CCAGATGTTT CAGCAGCTGA TGAAGCAGGT GACACAGCTG 
GCCATTGACA CGGAGGAACG CCTCAAAGGA GTTATTGACC TCATCTTTGA GAAAGCCATT 
TCAGAGCCCA ACTTCTCTGT GGCTTATGCC AACATGTGCC GCTGCCTCAT GGCGCTGAAA 
GTGCCCACTA CAGAAAAGCC AACAGTAACT GTGAATTTTC GAAAACTGTT GTTAAATCGG 
TGCCAGAAGG AATTTGAGAA AGACAAAGAT GATGATGAAG TTTTTGAAAA AAAGCAAAAA 
GAGATGGATG AAGCTGCTAC GGCAGAAGAA CGGGGACGCC TGAAAGAAGA GCTAGAAGAA 
GCCCGGGACA TAGCTAGGCG GCGCTCTTTA GGGAATATCA AGTTTATTGG AGAGTTATTC 
AAGTTGAAGA TGTTAACAGA AGCAATAATG CATGACTGTG TGGTCAAGCT ACTTAAGAAC 
CATGATGAGG AGTCCCTGGA ATGCCTCTGC CGCCTCCTCA CCACTATAGG CAAAGACCTG 
GACTTTGCAA AAGCCAAGCC TCGAATGGAT CAGTATTTCA ACCAAATGGA AAAAATCATT 
AAAGAAAAGA AGACCTCATC TCGCATCCGT TTTATGCTGC AGGATGTACT CGATCTGCGG 
CAGAGTAATT GGGTGCCACG CCGAGGGGAT CAGGGTCCTA AGACTATTGA TCAAATTCAC 
AAGGAGGCTG AGATGGAAGA GCACCGAGAA CATATTAAAG TACAGCAGCT CATGGCCAAG 
GGCAGCGACA AACGTCGGGG TGGCCCTCCA GGCCCGCCTA TCAACCGTGG CCTTCCACTT 
GTAGATGATG GTGGCTGGAA TACAGTCCCC ATTAGCAAAG GCAGTCGCCC CATTGACACC 
TCACGGCTCA CCAAGATCAC AAAGCCTGGT TCCATTGATT CTAACAACCA GCTTTTTGCA 
CCTGGAGGAC GACTGAGTTG GGGCAAGGGC AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA 
GACACAGCAT CGGAAGCTAC TCGTCCAGCT ACCTTGAATC GCTTTTCTGC TCTTCAACAA 
ACATTACCCG CAGAGAACAC AGATAACAGA CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG 
GAACGAGGTG AGAAAGCTGG GGACCGGGGA GACCGACTAG AGCGGAGTGA GCGGGGAGGT 
GACCGTGGGG ACCGACTTGA TCGTGCCAGA ACACCTGCCA CCAAGCGAAG TTTTAGCAAA 
GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA TCCCAGCCTG AGGGACTCCG CAAGGCAGCT 
AGCCTCACAG AGGATCGTGG TCGGGATCCT GTGAAGCGGG AAGCCACTCT ACCTCCAGTG 
AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT GAGGTGGAGA AGAAATCTAA GGCCATCATT 
GAGGAATATC TCCATCTCAA TGACATGAAG GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT 
TCACCCTCCT TGCTCTTCAT CTTCGTACGG CTTGGTATCG AGTCCACGCT GGAGCGTAGT 
ACCATTGCTC GTGAGCATAT GGGGCGACTA CTGCACCAAC TGCTCTGTGC GGGGCACCTC 
TCTACTGCCC AGTACTATCA AGGGCTGTAT GAAACACTAG AATTGGCTGA GGACATGGAA 
ATTGATATCC CTCATGTATG GCTTTACCTG GCAGAACTGA TTACACCTAT TCTCCAGGAA 
GATGGGGTGC CCATGGGAGA GCTCTTCAGG GAAATTACGA AGCCTCTGAG ACCCATGGGC 
AAAGCTACTT CTTTATTGCT GGAGATCCTG GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA 
AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG CTGAGCTGGA GGGAATTTCT AGCAGAGGGC 
CAAGACGTTG GCTCATTTGT GGCTGAAAAG AAGGTGGAAT ATACCTTGGG AGAAGAATCT 
GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG 
CTGAAGGACG GCGGCAGTAA TCAGCGTGTG TTCGACTGGA TAGACGCCAA CCTAAATGAG 
CAGCAGATAG CATCCAATAC ATTAGTTCGA GCCCTCATGA CAACTGTCTG TTATTCTGCA 
ATTATCTTTG AGACTCCTCT CCGAGTGGAT GTTCAAGTGT TGAAAGTGCG AGCAAGACTG 
CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG GAGCTACAAG CACTCTATGC TCTCCAGGCC 
CTTGTAGTGA CCTTAGAACA GCCTGCCAAC CTGCTTCGGA TGTTCTTTGA TGCTCTATAT 
GATGAGGACG TGGTGAAGGA AGACGCCTTC TACAGCTGGG AGAGCAGCAA GGATCCTGCT 
GAACAGCAGG GCAAGGGTGT GGCCCTTAAA TCTGTCACAG CATTCTTCAA TTGGCTTCGT 
GAGGCTGAGG ACGAGGAGTC TGATCACAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104883.1
CDS	249..5021
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_030249023.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249023.1

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG 
CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA 
GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT 
TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA 
GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC 
TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG 
CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG 
CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT 
TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC 
CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG 
ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC 
GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT 
TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC 
AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT 
CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG 
CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT 
GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT 
GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT 
GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT 
GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG 
GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT 
GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT 
CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG 
CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC 
ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT 
GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC 
ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA 
ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC 
ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT 
GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG GCTGGCCTGG GACCCAGGCG CTCTCAGCAG 
GGCCCACGAA AGGAAACTCG CAAGATCATT TCCTCAGTGA TAATGACTGA AGACATAAAA 
CTAAATAAAG CAGAGAAGGC TTGGAAACCC AGTAGCAAAC GGACAGCAGC TGATAAGGAT 
CGAGGGGAAG AGGATGCTGA TGGAAGCAAG ACCCAGGACC TGTTCCGCAG GGTACGCTCC 
ATCCTGAATA AGCTGACACC CCAGATGTTT CAGCAGCTGA TGAAGCAGGT GACACAGCTG 
GCCATTGACA CGGAGGAACG CCTCAAAGGA GTTATTGACC TCATCTTTGA GAAAGCCATT 
TCAGAGCCCA ACTTCTCTGT GGCTTATGCC AACATGTGCC GCTGCCTCAT GGCGCTGAAA 
GTGCCCACTA CAGAAAAGCC AACAGTAACT GTGAATTTTC GAAAACTGTT GTTAAATCGG 
TGCCAGAAGG AATTTGAGAA AGACAAAGAT GATGATGAAG TTTTTGAAAA AAAGCAAAAA 
GAGATGGATG AAGCTGCTAC GGCAGAAGAA CGGGGACGCC TGAAAGAAGA GCTAGAAGAA 
GCCCGGGACA TAGCTAGGCG GCGCTCTTTA GGGAATATCA AGTTTATTGG AGAGTTATTC 
AAGTTGAAGA TGTTAACAGA AGCAATAATG CATGACTGTG TGGTCAAGCT ACTTAAGAAC 
CATGATGAGG AGTCCCTGGA ATGCCTCTGC CGCCTCCTCA CCACTATAGG CAAAGACCTG 
GACTTTGCAA AAGCCAAGCC TCGAATGGAT CAGTATTTCA ACCAAATGGA AAAAATCATT 
AAAGAAAAGA AGACCTCATC TCGCATCCGT TTTATGCTGC AGGATGTACT CGATCTGCGG 
CAGAGTAATT GGGTGCCACG CCGAGGGGAT CAGGGTCCTA AGACTATTGA TCAAATTCAC 
AAGGAGGCTG AGATGGAAGA GCACCGAGAA CATATTAAAG TACAGCAGCT CATGGCCAAG 
GGCAGCGACA AACGTCGGGG TGGCCCTCCA GGCCCGCCTA TCAACCGTGG CCTTCCACTT 
GTAGATGATG GTGGCTGGAA TACAGTCCCC ATTAGCAAAG GCAGTCGCCC CATTGACACC 
TCACGGCTCA CCAAGATCAC AAAGCCTGGT TCCATTGATT CTAACAACCA GCTTTTTGCA 
CCTGGAGGAC GACTGAGTTG GGGCAAGGGC AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA 
GACACAGCAT CGGAAGCTAC TCGTCCAGCT ACCTTGAATC GCTTTTCTGC TCTTCAACAA 
ACATTACCCG CAGAGAACAC AGATAACAGA CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG 
GAACGAGGTG AGAAAGCTGG GGACCGGGGA GACCGACTAG AGCGGAGTGA GCGGGGAGGT 
GACCGTGGGG ACCGACTTGA TCGTGCCAGA ACACCTGCCA CCAAGCGAAG TTTTAGCAAA 
GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA TCCCAGCCTG AGGGACTCCG CAAGGCAGCT 
AGCCTCACAG AGGATCGTGG TCGGGATCCT GTGAAGCGGG AAGCCACTCT ACCTCCAGTG 
AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT GAGGTGGAGA AGAAATCTAA GGCCATCATT 
GAGGAATATC TCCATCTCAA TGACATGAAG GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT 
TCACCCTCCT TGCTCTTCAT CTTCGTACGG CTTGGTATCG AGTCCACGCT GGAGCGTAGT 
ACCATTGCTC GTGAGCATAT GGGGCGACTA CTGCACCAAC TGCTCTGTGC GGGGCACCTC 
TCTACTGCCC AGTACTATCA AGGGCTGTAT GAAACACTAG AATTGGCTGA GGACATGGAA 
ATTGATATCC CTCATGTATG GCTTTACCTG GCAGAACTGA TTACACCTAT TCTCCAGGAA 
GATGGGGTGC CCATGGGAGA GCTCTTCAGG GAAATTACGA AGCCTCTGAG ACCCATGGGC 
AAAGCTACTT CTTTATTGCT GGAGATCCTG GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA 
AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG CTGAGCTGGA GGGAATTTCT AGCAGAGGGC 
CAAGACGTTG GCTCATTTGT GGCTGAAAAG AAGGTGGAAT ATACCTTGGG AGAAGAATCT 
GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG 
CTGAAGGACG GCGGCAGTAA TCAGCGTGTG TTCGACTGGA TAGACGCCAA CCTAAATGAG 
CAGCAGATAG CATCCAATAC ATTAGTTCGA GCCCTCATGA CAACTGTCTG TTATTCTGCA 
ATTATCTTTG AGACTCCTCT CCGAGTGGAT GTTCAAGTGT TGAAAGTGCG AGCAAGACTG 
CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG GAGCTACAAG CACTCTATGC TCTCCAGGCC 
CTTGTAGTGA CCTTAGAACA GCCTGCCAAC CTGCTTCGGA TGTTCTTTGA TGCTCTATAT 
GATGAGGACG TGGTGAAGGA AGACGCCTTC TACAGCTGGG AGAGCAGCAA GGATCCTGCT 
GAACAGCAGG GCAAGGGTGT GGCCCTTAAA TCTGTCACAG CATTCTTCAA TTGGCTTCGT 
GAGGCTGAGG ACGAGGAGTC TGATCACAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_030249024.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104884.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA.

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CloneID	OMu86906
Clone ID Related Accession (Same CDS sequence)	XM_030249024.1
Accession Version	XM_030249024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGCAGTAC CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT 
CCAGGTGCAA GCCCTACCGA GTTTGGGACC TATGCTGGAG CCTATTACCC AGCCCAAGGT 
GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT GCCCCAGTTT TGATGAACCA GCCACCCCAG 
ATTGCTCCTA AGAGGGAACG GAAAACTATC CGAATTCGAG ACCCAAACCA AGGAGGGAAG 
GATATCACAG AAGAGATCAT GTCTGGGGCC CGCACTGCCT CCACACCCAC TCCTCCCCAG 
ACGGGAGGCA GTCTGGAGCC TCAACCCAAT GGGGAGTCGC CTCAGGTTGC TGTCATTATC 
CGGCCAGATG ACCGGTCGCA GGGAGCAGCC ATTGGGGGGC GGCCAGGACT GCCTGGCCCA 
GAGCATAGCC CTGGCACAGA ATCTCAGCCT TCGTCGCCTT CTCCAACCCC ATCACCACCC 
CCAATTTTGG AGCCGGGGTC TGAGTCTAAT CTTGGAGTCC TCTCTATTCC TGGGGACACT 
ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT 
GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA 
GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC 
ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG 
GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT 
GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA 
GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG 
AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA 
GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA 
GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC 
CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC 
CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC 
TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG 
TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC 
AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG 
CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT 
CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT 
GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC TCTCAGCAGG GCCCACGAAA GGAAACTCGC 
AAGATCATTT CCTCAGTGAT AATGACTGAA GACATAAAAC TAAATAAAGC AGAGAAGGCT 
TGGAAACCCA GTAGCAAACG GACAGCAGCT GATAAGGATC GAGGGGAAGA GGATGCTGAT 
GGAAGCAAGA CCCAGGACCT GTTCCGCAGG GTACGCTCCA TCCTGAATAA GCTGACACCC 
CAGATGTTTC AGCAGCTGAT GAAGCAGGTG ACACAGCTGG CCATTGACAC GGAGGAACGC 
CTCAAAGGAG TTATTGACCT CATCTTTGAG AAAGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCTTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTAACTG TGAATTTTCG AAAACTGTTG TTAAATCGGT GCCAGAAGGA ATTTGAGAAA 
GACAAAGATG ATGATGAAGT TTTTGAAAAA AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAAGAAC GGGGACGCCT GAAAGAAGAG CTAGAAGAAG CCCGGGACAT AGCTAGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTATTCA AGTTGAAGAT GTTAACAGAA 
GCAATAATGC ATGACTGTGT GGTCAAGCTA CTTAAGAACC ATGATGAGGA GTCCCTGGAA 
TGCCTCTGCC GCCTCCTCAC CACTATAGGC AAAGACCTGG ACTTTGCAAA AGCCAAGCCT 
CGAATGGATC AGTATTTCAA CCAAATGGAA AAAATCATTA AAGAAAAGAA GACCTCATCT 
CGCATCCGTT TTATGCTGCA GGATGTACTC GATCTGCGGC AGAGTAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCTAA GACTATTGAT CAAATTCACA AGGAGGCTGA GATGGAAGAG 
CACCGAGAAC ATATTAAAGT ACAGCAGCTC ATGGCCAAGG GCAGCGACAA ACGTCGGGGT 
GGCCCTCCAG GCCCGCCTAT CAACCGTGGC CTTCCACTTG TAGATGATGG TGGCTGGAAT 
ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC ATTGACACCT CACGGCTCAC CAAGATCACA 
AAGCCTGGTT CCATTGATTC TAACAACCAG CTTTTTGCAC CTGGAGGACG ACTGAGTTGG 
GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC AAGCCCTCAG ACACAGCATC GGAAGCTACT 
CGTCCAGCTA CCTTGAATCG CTTTTCTGCT CTTCAACAAA CATTACCCGC AGAGAACACA 
GATAACAGAC GTGTTGTACA GAGGAGTAGC TTGAGCCGGG AACGAGGTGA GAAAGCTGGG 
GACCGGGGAG ACCGACTAGA GCGGAGTGAG CGGGGAGGTG ACCGTGGGGA CCGACTTGAT 
CGTGCCAGAA CACCTGCCAC CAAGCGAAGT TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA 
GAGCGGCCAT CCCAGCCTGA GGGACTCCGC AAGGCAGCTA GCCTCACAGA GGATCGTGGT 
CGGGATCCTG TGAAGCGGGA AGCCACTCTA CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG 
TCTGTGGATG AGGTGGAGAA GAAATCTAAG GCCATCATTG AGGAATATCT CCATCTCAAT 
GACATGAAGG AGGCAGTACA GTGTGTCCAG GAGCTGGCTT CACCCTCCTT GCTCTTCATC 
TTCGTACGGC TTGGTATCGA GTCCACGCTG GAGCGTAGTA CCATTGCTCG TGAGCATATG 
GGGCGACTAC TGCACCAACT GCTCTGTGCG GGGCACCTCT CTACTGCCCA GTACTATCAA 
GGGCTGTATG AAACACTAGA ATTGGCTGAG GACATGGAAA TTGATATCCC TCATGTATGG 
CTTTACCTGG CAGAACTGAT TACACCTATT CTCCAGGAAG ATGGGGTGCC CATGGGAGAG 
CTCTTCAGGG AAATTACGAA GCCTCTGAGA CCCATGGGCA AAGCTACTTC TTTATTGCTG 
GAGATCCTGG GTCTCTTATG CAAGAGCATG GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA 
GAGGCTGGGC TGAGCTGGAG GGAATTTCTA GCAGAGGGCC AAGACGTTGG CTCATTTGTG 
GCTGAAAAGA AGGTGGAATA TACCTTGGGA GAAGAATCTG AAGCTCCTGG CCAGAGGACA 
CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA GAGAAGCTGC TGAAGGACGG CGGCAGTAAT 
CAGCGTGTGT TCGACTGGAT AGACGCCAAC CTAAATGAGC AGCAGATAGC ATCCAATACA 
TTAGTTCGAG CCCTCATGAC AACTGTCTGT TATTCTGCAA TTATCTTTGA GACTCCTCTC 
CGAGTGGATG TTCAAGTGTT GAAAGTGCGA GCAAGACTGC TGCAGAAATA CCTGTGTGAT 
GAGCAGAAGG AGCTACAAGC ACTCTATGCT CTCCAGGCCC TTGTAGTGAC CTTAGAACAG 
CCTGCCAACC TGCTTCGGAT GTTCTTTGAT GCTCTATATG ATGAGGACGT GGTGAAGGAA 
GACGCCTTCT ACAGCTGGGA GAGCAGCAAG GATCCTGCTG AACAGCAGGG CAAGGGTGTG 
GCCCTTAAAT CTGTCACAGC ATTCTTCAAT TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT 
GATCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104884.1
CDS	249..5000
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_030249024.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249024.1

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGCAGTAC CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT 
CCAGGTGCAA GCCCTACCGA GTTTGGGACC TATGCTGGAG CCTATTACCC AGCCCAAGGT 
GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT GCCCCAGTTT TGATGAACCA GCCACCCCAG 
ATTGCTCCTA AGAGGGAACG GAAAACTATC CGAATTCGAG ACCCAAACCA AGGAGGGAAG 
GATATCACAG AAGAGATCAT GTCTGGGGCC CGCACTGCCT CCACACCCAC TCCTCCCCAG 
ACGGGAGGCA GTCTGGAGCC TCAACCCAAT GGGGAGTCGC CTCAGGTTGC TGTCATTATC 
CGGCCAGATG ACCGGTCGCA GGGAGCAGCC ATTGGGGGGC GGCCAGGACT GCCTGGCCCA 
GAGCATAGCC CTGGCACAGA ATCTCAGCCT TCGTCGCCTT CTCCAACCCC ATCACCACCC 
CCAATTTTGG AGCCGGGGTC TGAGTCTAAT CTTGGAGTCC TCTCTATTCC TGGGGACACT 
ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT 
GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA 
GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC 
ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG 
GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT 
GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA 
GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG 
AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA 
GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA 
GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC 
CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC 
CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC 
TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG 
TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC 
AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG 
CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT 
CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT 
GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC TCTCAGCAGG GCCCACGAAA GGAAACTCGC 
AAGATCATTT CCTCAGTGAT AATGACTGAA GACATAAAAC TAAATAAAGC AGAGAAGGCT 
TGGAAACCCA GTAGCAAACG GACAGCAGCT GATAAGGATC GAGGGGAAGA GGATGCTGAT 
GGAAGCAAGA CCCAGGACCT GTTCCGCAGG GTACGCTCCA TCCTGAATAA GCTGACACCC 
CAGATGTTTC AGCAGCTGAT GAAGCAGGTG ACACAGCTGG CCATTGACAC GGAGGAACGC 
CTCAAAGGAG TTATTGACCT CATCTTTGAG AAAGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCTTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTAACTG TGAATTTTCG AAAACTGTTG TTAAATCGGT GCCAGAAGGA ATTTGAGAAA 
GACAAAGATG ATGATGAAGT TTTTGAAAAA AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAAGAAC GGGGACGCCT GAAAGAAGAG CTAGAAGAAG CCCGGGACAT AGCTAGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTATTCA AGTTGAAGAT GTTAACAGAA 
GCAATAATGC ATGACTGTGT GGTCAAGCTA CTTAAGAACC ATGATGAGGA GTCCCTGGAA 
TGCCTCTGCC GCCTCCTCAC CACTATAGGC AAAGACCTGG ACTTTGCAAA AGCCAAGCCT 
CGAATGGATC AGTATTTCAA CCAAATGGAA AAAATCATTA AAGAAAAGAA GACCTCATCT 
CGCATCCGTT TTATGCTGCA GGATGTACTC GATCTGCGGC AGAGTAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCTAA GACTATTGAT CAAATTCACA AGGAGGCTGA GATGGAAGAG 
CACCGAGAAC ATATTAAAGT ACAGCAGCTC ATGGCCAAGG GCAGCGACAA ACGTCGGGGT 
GGCCCTCCAG GCCCGCCTAT CAACCGTGGC CTTCCACTTG TAGATGATGG TGGCTGGAAT 
ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC ATTGACACCT CACGGCTCAC CAAGATCACA 
AAGCCTGGTT CCATTGATTC TAACAACCAG CTTTTTGCAC CTGGAGGACG ACTGAGTTGG 
GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC AAGCCCTCAG ACACAGCATC GGAAGCTACT 
CGTCCAGCTA CCTTGAATCG CTTTTCTGCT CTTCAACAAA CATTACCCGC AGAGAACACA 
GATAACAGAC GTGTTGTACA GAGGAGTAGC TTGAGCCGGG AACGAGGTGA GAAAGCTGGG 
GACCGGGGAG ACCGACTAGA GCGGAGTGAG CGGGGAGGTG ACCGTGGGGA CCGACTTGAT 
CGTGCCAGAA CACCTGCCAC CAAGCGAAGT TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA 
GAGCGGCCAT CCCAGCCTGA GGGACTCCGC AAGGCAGCTA GCCTCACAGA GGATCGTGGT 
CGGGATCCTG TGAAGCGGGA AGCCACTCTA CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG 
TCTGTGGATG AGGTGGAGAA GAAATCTAAG GCCATCATTG AGGAATATCT CCATCTCAAT 
GACATGAAGG AGGCAGTACA GTGTGTCCAG GAGCTGGCTT CACCCTCCTT GCTCTTCATC 
TTCGTACGGC TTGGTATCGA GTCCACGCTG GAGCGTAGTA CCATTGCTCG TGAGCATATG 
GGGCGACTAC TGCACCAACT GCTCTGTGCG GGGCACCTCT CTACTGCCCA GTACTATCAA 
GGGCTGTATG AAACACTAGA ATTGGCTGAG GACATGGAAA TTGATATCCC TCATGTATGG 
CTTTACCTGG CAGAACTGAT TACACCTATT CTCCAGGAAG ATGGGGTGCC CATGGGAGAG 
CTCTTCAGGG AAATTACGAA GCCTCTGAGA CCCATGGGCA AAGCTACTTC TTTATTGCTG 
GAGATCCTGG GTCTCTTATG CAAGAGCATG GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA 
GAGGCTGGGC TGAGCTGGAG GGAATTTCTA GCAGAGGGCC AAGACGTTGG CTCATTTGTG 
GCTGAAAAGA AGGTGGAATA TACCTTGGGA GAAGAATCTG AAGCTCCTGG CCAGAGGACA 
CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA GAGAAGCTGC TGAAGGACGG CGGCAGTAAT 
CAGCGTGTGT TCGACTGGAT AGACGCCAAC CTAAATGAGC AGCAGATAGC ATCCAATACA 
TTAGTTCGAG CCCTCATGAC AACTGTCTGT TATTCTGCAA TTATCTTTGA GACTCCTCTC 
CGAGTGGATG TTCAAGTGTT GAAAGTGCGA GCAAGACTGC TGCAGAAATA CCTGTGTGAT 
GAGCAGAAGG AGCTACAAGC ACTCTATGCT CTCCAGGCCC TTGTAGTGAC CTTAGAACAG 
CCTGCCAACC TGCTTCGGAT GTTCTTTGAT GCTCTATATG ATGAGGACGT GGTGAAGGAA 
GACGCCTTCT ACAGCTGGGA GAGCAGCAAG GATCCTGCTG AACAGCAGGG CAAGGGTGTG 
GCCCTTAAAT CTGTCACAGC ATTCTTCAAT TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT 
GATCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521940.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522003.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA.

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CloneID	OMu14547
Clone ID Related Accession (Same CDS sequence)	XM_006521940.4 , XM_006521940.3
Accession Version	XM_006521940.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521940.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC 
TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA 
GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT 
GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG 
GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG 
ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG 
AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG 
GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG 
TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA 
AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG 
CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA 
GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA 
CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC 
AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA 
AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC 
GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT 
CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC 
ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAACCGTGGC 
CTTCCACTTG TAGATGATGG TGGCTGGAAT ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC 
ATTGACACCT CACGGCTCAC CAAGATCACA AAGCCTGGTT CCATTGATTC TAACAACCAG 
CTTTTTGCAC CTGGAGGACG ACTGAGTTGG GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC 
AAGCCCTCAG ACACAGCATC GGAAGCTACT CGTCCAGCTA CCTTGAATCG CTTTTCTGCT 
CTTCAACAAA CATTACCCGC AGAGAACACA GATAACAGAC GTGTTGTACA GAGGAGTAGC 
TTGAGCCGGG AACGAGGTGA GAAAGCTGGG GACCGGGGAG ACCGACTAGA GCGGAGTGAG 
CGGGGAGGTG ACCGTGGGGA CCGACTTGAT CGTGCCAGAA CACCTGCCAC CAAGCGAAGT 
TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA GAGCGGCCAT CCCAGCCTGA GGGACTCCGC 
AAGGCAGCTA GCCTCACAGA GGATCGTGGT CGGGATCCTG TGAAGCGGGA AGCCACTCTA 
CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG TCTGTGGATG AGGTGGAGAA GAAATCTAAG 
GCCATCATTG AGGAATATCT CCATCTCAAT GACATGAAGG AGGCAGTACA GTGTGTCCAG 
GAGCTGGCTT CACCCTCCTT GCTCTTCATC TTCGTACGGC TTGGTATCGA GTCCACGCTG 
GAGCGTAGTA CCATTGCTCG TGAGCATATG GGGCGACTAC TGCACCAACT GCTCTGTGCG 
GGGCACCTCT CTACTGCCCA GTACTATCAA GGGCTGTATG AAACACTAGA ATTGGCTGAG 
GACATGGAAA TTGATATCCC TCATGTATGG CTTTACCTGG CAGAACTGAT TACACCTATT 
CTCCAGGAAG ATGGGGTGCC CATGGGAGAG CTCTTCAGGG AAATTACGAA GCCTCTGAGA 
CCCATGGGCA AAGCTACTTC TTTATTGCTG GAGATCCTGG GTCTCTTATG CAAGAGCATG 
GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA GAGGCTGGGC TGAGCTGGAG GGAATTTCTA 
GCAGAGGGCC AAGACGTTGG CTCATTTGTG GCTGAAAAGA AGGTGGAATA TACCTTGGGA 
GAAGAATCTG AAGCTCCTGG CCAGAGGACA CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA 
GAGAAGCTGC TGAAGGACGG CGGCAGTAAT CAGCGTGTGT TCGACTGGAT AGACGCCAAC 
CTAAATGAGC AGCAGATAGC ATCCAATACA TTAGTTCGAG CCCTCATGAC AACTGTCTGT 
TATTCTGCAA TTATCTTTGA GACTCCTCTC CGAGTGGATG TTCAAGTGTT GAAAGTGCGA 
GCAAGACTGC TGCAGAAATA CCTGTGTGAT GAGCAGAAGG AGCTACAAGC ACTCTATGCT 
CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTGCCAACC TGCTTCGGAT GTTCTTTGAT 
GCTCTATATG ATGAGGACGT GGTGAAGGAA GACGCCTTCT ACAGCTGGGA GAGCAGCAAG 
GATCCTGCTG AACAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC ATTCTTCAAT 
TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT GATCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522003.1
CDS	220..5001
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521940.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521940.3

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC 
TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA 
GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT 
GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG 
GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG 
ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG 
AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG 
GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG 
TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA 
AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG 
CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA 
GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA 
CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC 
AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA 
AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC 
GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT 
CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC 
ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAACCGTGGC 
CTTCCACTTG TAGATGATGG TGGCTGGAAT ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC 
ATTGACACCT CACGGCTCAC CAAGATCACA AAGCCTGGTT CCATTGATTC TAACAACCAG 
CTTTTTGCAC CTGGAGGACG ACTGAGTTGG GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC 
AAGCCCTCAG ACACAGCATC GGAAGCTACT CGTCCAGCTA CCTTGAATCG CTTTTCTGCT 
CTTCAACAAA CATTACCCGC AGAGAACACA GATAACAGAC GTGTTGTACA GAGGAGTAGC 
TTGAGCCGGG AACGAGGTGA GAAAGCTGGG GACCGGGGAG ACCGACTAGA GCGGAGTGAG 
CGGGGAGGTG ACCGTGGGGA CCGACTTGAT CGTGCCAGAA CACCTGCCAC CAAGCGAAGT 
TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA GAGCGGCCAT CCCAGCCTGA GGGACTCCGC 
AAGGCAGCTA GCCTCACAGA GGATCGTGGT CGGGATCCTG TGAAGCGGGA AGCCACTCTA 
CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG TCTGTGGATG AGGTGGAGAA GAAATCTAAG 
GCCATCATTG AGGAATATCT CCATCTCAAT GACATGAAGG AGGCAGTACA GTGTGTCCAG 
GAGCTGGCTT CACCCTCCTT GCTCTTCATC TTCGTACGGC TTGGTATCGA GTCCACGCTG 
GAGCGTAGTA CCATTGCTCG TGAGCATATG GGGCGACTAC TGCACCAACT GCTCTGTGCG 
GGGCACCTCT CTACTGCCCA GTACTATCAA GGGCTGTATG AAACACTAGA ATTGGCTGAG 
GACATGGAAA TTGATATCCC TCATGTATGG CTTTACCTGG CAGAACTGAT TACACCTATT 
CTCCAGGAAG ATGGGGTGCC CATGGGAGAG CTCTTCAGGG AAATTACGAA GCCTCTGAGA 
CCCATGGGCA AAGCTACTTC TTTATTGCTG GAGATCCTGG GTCTCTTATG CAAGAGCATG 
GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA GAGGCTGGGC TGAGCTGGAG GGAATTTCTA 
GCAGAGGGCC AAGACGTTGG CTCATTTGTG GCTGAAAAGA AGGTGGAATA TACCTTGGGA 
GAAGAATCTG AAGCTCCTGG CCAGAGGACA CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA 
GAGAAGCTGC TGAAGGACGG CGGCAGTAAT CAGCGTGTGT TCGACTGGAT AGACGCCAAC 
CTAAATGAGC AGCAGATAGC ATCCAATACA TTAGTTCGAG CCCTCATGAC AACTGTCTGT 
TATTCTGCAA TTATCTTTGA GACTCCTCTC CGAGTGGATG TTCAAGTGTT GAAAGTGCGA 
GCAAGACTGC TGCAGAAATA CCTGTGTGAT GAGCAGAAGG AGCTACAAGC ACTCTATGCT 
CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTGCCAACC TGCTTCGGAT GTTCTTTGAT 
GCTCTATATG ATGAGGACGT GGTGAAGGAA GACGCCTTCT ACAGCTGGGA GAGCAGCAAG 
GATCCTGCTG AACAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC ATTCTTCAAT 
TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT GATCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521935.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521998.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X1, mRNA.

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CloneID	OMu51986
Clone ID Related Accession (Same CDS sequence)	XM_006521935.1 , XM_006521936.2
Accession Version	XM_006521935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521998.1
CDS	249..5054
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521935.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521935.1

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521936.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521999.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu51986
Clone ID Related Accession (Same CDS sequence)	XM_006521935.1 , XM_006521936.2
Accession Version	XM_006521936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521999.1
CDS	137..4942
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521936.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521936.2

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521938.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522001.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA.

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CloneID	OMu51987
Clone ID Related Accession (Same CDS sequence)	XM_006521938.1 , XM_006521939.1 , XM_006521938.2
Accession Version	XM_006521938.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522001.1
CDS	249..5033
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521938.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521938.1

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521939.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522002.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X5, mRNA.

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CloneID	OMu51987
Clone ID Related Accession (Same CDS sequence)	XM_006521938.1 , XM_006521939.1 , XM_006521938.2
Accession Version	XM_006521939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522002.1
CDS	192..4976
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521939.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521939.1

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521942.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522005.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X9, mRNA.

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CloneID	OMu51989
Clone ID Related Accession (Same CDS sequence)	XM_006521942.1
Accession Version	XM_006521942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGCAGTAC CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT 
CCAGGTGCAA GCCCTACCGA GTTTGGGACC TATGCTGGAG CCTATTACCC AGCCCAAGGT 
GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT GCCCCAGTTT TGATGAACCA GCCACCCCAG 
ATTGCTCCTA AGAGGGAACG GAAAACTATC CGAATTCGAG ACCCAAACCA AGGAGGGAAG 
GATATCACAG AAGAGATCAT GTCTGGGGCC CGCACTGCCT CCACACCCAC TCCTCCCCAG 
ACGGGAGGCA GTCTGGAGCC TCAACCCAAT GGGGAGTCGC CTCAGGTTGC TGTCATTATC 
CGGCCAGATG ACCGGTCGCA GGGAGCAGCC ATTGGGGGGC GGCCAGGACT GCCTGGCCCA 
GAGCATAGCC CTGGCACAGA ATCTCAGCCT TCGTCGCCTT CTCCAACCCC ATCACCACCC 
CCAATTTTGG AGCCGGGGTC TGAGTCTAAT CTTGGAGTCC TCTCTATTCC TGGGGACACT 
ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT 
GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA 
GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC 
ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG 
GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT 
GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA 
GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG 
AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA 
GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA 
GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC 
CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC 
CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC 
TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG 
TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC 
AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG 
CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT 
CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT 
GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGC AGGCTGGCCT GGGACCCAGG CGCTCTCAGC AGGGCCCACG AAAGGAAACT 
CGCAAGATCA TTTCCTCAGT GATAATGACT GAAGACATAA AACTAAATAA AGCAGAGAAG 
GCTTGGAAAC CCAGTAGCAA ACGGACAGCA GCTGATAAGG ATCGAGGGGA AGAGGATGCT 
GATGGAAGCA AGACCCAGGA CCTGTTCCGC AGGGTACGCT CCATCCTGAA TAAGCTGACA 
CCCCAGATGT TTCAGCAGCT GATGAAGCAG GTGACACAGC TGGCCATTGA CACGGAGGAA 
CGCCTCAAAG GAGTTATTGA CCTCATCTTT GAGAAAGCCA TTTCAGAGCC CAACTTCTCT 
GTGGCTTATG CCAACATGTG CCGCTGCCTC ATGGCGCTGA AAGTGCCCAC TACAGAAAAG 
CCAACAGTAA CTGTGAATTT TCGAAAACTG TTGTTAAATC GGTGCCAGAA GGAATTTGAG 
AAAGACAAAG ATGATGATGA AGTTTTTGAA AAAAAGCAAA AAGAGATGGA TGAAGCTGCT 
ACGGCAGAAG AACGGGGACG CCTGAAAGAA GAGCTAGAAG AAGCCCGGGA CATAGCTAGG 
CGGCGCTCTT TAGGGAATAT CAAGTTTATT GGAGAGTTAT TCAAGTTGAA GATGTTAACA 
GAAGCAATAA TGCATGACTG TGTGGTCAAG CTACTTAAGA ACCATGATGA GGAGTCCCTG 
GAATGCCTCT GCCGCCTCCT CACCACTATA GGCAAAGACC TGGACTTTGC AAAAGCCAAG 
CCTCGAATGG ATCAGTATTT CAACCAAATG GAAAAAATCA TTAAAGAAAA GAAGACCTCA 
TCTCGCATCC GTTTTATGCT GCAGGATGTA CTCGATCTGC GGCAGAGTAA TTGGGTGCCA 
CGCCGAGGGG ATCAGGGTCC TAAGACTATT GATCAAATTC ACAAGGAGGC TGAGATGGAA 
GAGCACCGAG AACATATTAA AGTACAGCAG CTCATGGCCA AGGGCAGCGA CAAACGTCGG 
GGTGGCCCTC CAGGCCCGCC TATCAACCGT GGCCTTCCAC TTGTAGATGA TGGTGGCTGG 
AATACAGTCC CCATTAGCAA AGGCAGTCGC CCCATTGACA CCTCACGGCT CACCAAGATC 
ACAAAGCCTG GTTCCATTGA TTCTAACAAC CAGCTTTTTG CACCTGGAGG ACGACTGAGT 
TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA GCCAAGCCCT CAGACACAGC ATCGGAAGCT 
ACTCGTCCAG CTACCTTGAA TCGCTTTTCT GCTCTTCAAC AAACATTACC CGCAGAGAAC 
ACAGATAACA GACGTGTTGT ACAGAGGAGT AGCTTGAGCC GGGAACGAGG TGAGAAAGCT 
GGGGACCGGG GAGACCGACT AGAGCGGAGT GAGCGGGGAG GTGACCGTGG GGACCGACTT 
GATCGTGCCA GAACACCTGC CACCAAGCGA AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT 
AGAGAGCGGC CATCCCAGCC TGAGGGACTC CGCAAGGCAG CTAGCCTCAC AGAGGATCGT 
GGTCGGGATC CTGTGAAGCG GGAAGCCACT CTACCTCCAG TGAGCCCTCC AAAGGCTGCG 
CTGTCTGTGG ATGAGGTGGA GAAGAAATCT AAGGCCATCA TTGAGGAATA TCTCCATCTC 
AATGACATGA AGGAGGCAGT ACAGTGTGTC CAGGAGCTGG CTTCACCCTC CTTGCTCTTC 
ATCTTCGTAC GGCTTGGTAT CGAGTCCACG CTGGAGCGTA GTACCATTGC TCGTGAGCAT 
ATGGGGCGAC TACTGCACCA ACTGCTCTGT GCGGGGCACC TCTCTACTGC CCAGTACTAT 
CAAGGGCTGT ATGAAACACT AGAATTGGCT GAGGACATGG AAATTGATAT CCCTCATGTA 
TGGCTTTACC TGGCAGAACT GATTACACCT ATTCTCCAGG AAGATGGGGT GCCCATGGGA 
GAGCTCTTCA GGGAAATTAC GAAGCCTCTG AGACCCATGG GCAAAGCTAC TTCTTTATTG 
CTGGAGATCC TGGGTCTCTT ATGCAAGAGC ATGGGTCCCA AAAAGGTGGG GATGCTGTGG 
CGAGAGGCTG GGCTGAGCTG GAGGGAATTT CTAGCAGAGG GCCAAGACGT TGGCTCATTT 
GTGGCTGAAA AGAAGGTGGA ATATACCTTG GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG 
ACACTTGCCT TTGAGGAGCT TCGTAGGCAG CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT 
AATCAGCGTG TGTTCGACTG GATAGACGCC AACCTAAATG AGCAGCAGAT AGCATCCAAT 
ACATTAGTTC GAGCCCTCAT GACAACTGTC TGTTATTCTG CAATTATCTT TGAGACTCCT 
CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG CGAGCAAGAC TGCTGCAGAA ATACCTGTGT 
GATGAGCAGA AGGAGCTACA AGCACTCTAT GCTCTCCAGG CCCTTGTAGT GACCTTAGAA 
CAGCCTGCCA ACCTGCTTCG GATGTTCTTT GATGCTCTAT ATGATGAGGA CGTGGTGAAG 
GAAGACGCCT TCTACAGCTG GGAGAGCAGC AAGGATCCTG CTGAACAGCA GGGCAAGGGT 
GTGGCCCTTA AATCTGTCAC AGCATTCTTC AATTGGCTTC GTGAGGCTGA GGACGAGGAG 
TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522005.1
CDS	249..5003
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521942.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521942.1

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGCAGTAC CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT 
CCAGGTGCAA GCCCTACCGA GTTTGGGACC TATGCTGGAG CCTATTACCC AGCCCAAGGT 
GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT GCCCCAGTTT TGATGAACCA GCCACCCCAG 
ATTGCTCCTA AGAGGGAACG GAAAACTATC CGAATTCGAG ACCCAAACCA AGGAGGGAAG 
GATATCACAG AAGAGATCAT GTCTGGGGCC CGCACTGCCT CCACACCCAC TCCTCCCCAG 
ACGGGAGGCA GTCTGGAGCC TCAACCCAAT GGGGAGTCGC CTCAGGTTGC TGTCATTATC 
CGGCCAGATG ACCGGTCGCA GGGAGCAGCC ATTGGGGGGC GGCCAGGACT GCCTGGCCCA 
GAGCATAGCC CTGGCACAGA ATCTCAGCCT TCGTCGCCTT CTCCAACCCC ATCACCACCC 
CCAATTTTGG AGCCGGGGTC TGAGTCTAAT CTTGGAGTCC TCTCTATTCC TGGGGACACT 
ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT 
GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA 
GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC 
ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG 
GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT 
GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA 
GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG 
AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA 
GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA 
GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC 
CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC 
CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC 
TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG 
TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC 
AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG 
CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT 
CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT 
GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGC AGGCTGGCCT GGGACCCAGG CGCTCTCAGC AGGGCCCACG AAAGGAAACT 
CGCAAGATCA TTTCCTCAGT GATAATGACT GAAGACATAA AACTAAATAA AGCAGAGAAG 
GCTTGGAAAC CCAGTAGCAA ACGGACAGCA GCTGATAAGG ATCGAGGGGA AGAGGATGCT 
GATGGAAGCA AGACCCAGGA CCTGTTCCGC AGGGTACGCT CCATCCTGAA TAAGCTGACA 
CCCCAGATGT TTCAGCAGCT GATGAAGCAG GTGACACAGC TGGCCATTGA CACGGAGGAA 
CGCCTCAAAG GAGTTATTGA CCTCATCTTT GAGAAAGCCA TTTCAGAGCC CAACTTCTCT 
GTGGCTTATG CCAACATGTG CCGCTGCCTC ATGGCGCTGA AAGTGCCCAC TACAGAAAAG 
CCAACAGTAA CTGTGAATTT TCGAAAACTG TTGTTAAATC GGTGCCAGAA GGAATTTGAG 
AAAGACAAAG ATGATGATGA AGTTTTTGAA AAAAAGCAAA AAGAGATGGA TGAAGCTGCT 
ACGGCAGAAG AACGGGGACG CCTGAAAGAA GAGCTAGAAG AAGCCCGGGA CATAGCTAGG 
CGGCGCTCTT TAGGGAATAT CAAGTTTATT GGAGAGTTAT TCAAGTTGAA GATGTTAACA 
GAAGCAATAA TGCATGACTG TGTGGTCAAG CTACTTAAGA ACCATGATGA GGAGTCCCTG 
GAATGCCTCT GCCGCCTCCT CACCACTATA GGCAAAGACC TGGACTTTGC AAAAGCCAAG 
CCTCGAATGG ATCAGTATTT CAACCAAATG GAAAAAATCA TTAAAGAAAA GAAGACCTCA 
TCTCGCATCC GTTTTATGCT GCAGGATGTA CTCGATCTGC GGCAGAGTAA TTGGGTGCCA 
CGCCGAGGGG ATCAGGGTCC TAAGACTATT GATCAAATTC ACAAGGAGGC TGAGATGGAA 
GAGCACCGAG AACATATTAA AGTACAGCAG CTCATGGCCA AGGGCAGCGA CAAACGTCGG 
GGTGGCCCTC CAGGCCCGCC TATCAACCGT GGCCTTCCAC TTGTAGATGA TGGTGGCTGG 
AATACAGTCC CCATTAGCAA AGGCAGTCGC CCCATTGACA CCTCACGGCT CACCAAGATC 
ACAAAGCCTG GTTCCATTGA TTCTAACAAC CAGCTTTTTG CACCTGGAGG ACGACTGAGT 
TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA GCCAAGCCCT CAGACACAGC ATCGGAAGCT 
ACTCGTCCAG CTACCTTGAA TCGCTTTTCT GCTCTTCAAC AAACATTACC CGCAGAGAAC 
ACAGATAACA GACGTGTTGT ACAGAGGAGT AGCTTGAGCC GGGAACGAGG TGAGAAAGCT 
GGGGACCGGG GAGACCGACT AGAGCGGAGT GAGCGGGGAG GTGACCGTGG GGACCGACTT 
GATCGTGCCA GAACACCTGC CACCAAGCGA AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT 
AGAGAGCGGC CATCCCAGCC TGAGGGACTC CGCAAGGCAG CTAGCCTCAC AGAGGATCGT 
GGTCGGGATC CTGTGAAGCG GGAAGCCACT CTACCTCCAG TGAGCCCTCC AAAGGCTGCG 
CTGTCTGTGG ATGAGGTGGA GAAGAAATCT AAGGCCATCA TTGAGGAATA TCTCCATCTC 
AATGACATGA AGGAGGCAGT ACAGTGTGTC CAGGAGCTGG CTTCACCCTC CTTGCTCTTC 
ATCTTCGTAC GGCTTGGTAT CGAGTCCACG CTGGAGCGTA GTACCATTGC TCGTGAGCAT 
ATGGGGCGAC TACTGCACCA ACTGCTCTGT GCGGGGCACC TCTCTACTGC CCAGTACTAT 
CAAGGGCTGT ATGAAACACT AGAATTGGCT GAGGACATGG AAATTGATAT CCCTCATGTA 
TGGCTTTACC TGGCAGAACT GATTACACCT ATTCTCCAGG AAGATGGGGT GCCCATGGGA 
GAGCTCTTCA GGGAAATTAC GAAGCCTCTG AGACCCATGG GCAAAGCTAC TTCTTTATTG 
CTGGAGATCC TGGGTCTCTT ATGCAAGAGC ATGGGTCCCA AAAAGGTGGG GATGCTGTGG 
CGAGAGGCTG GGCTGAGCTG GAGGGAATTT CTAGCAGAGG GCCAAGACGT TGGCTCATTT 
GTGGCTGAAA AGAAGGTGGA ATATACCTTG GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG 
ACACTTGCCT TTGAGGAGCT TCGTAGGCAG CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT 
AATCAGCGTG TGTTCGACTG GATAGACGCC AACCTAAATG AGCAGCAGAT AGCATCCAAT 
ACATTAGTTC GAGCCCTCAT GACAACTGTC TGTTATTCTG CAATTATCTT TGAGACTCCT 
CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG CGAGCAAGAC TGCTGCAGAA ATACCTGTGT 
GATGAGCAGA AGGAGCTACA AGCACTCTAT GCTCTCCAGG CCCTTGTAGT GACCTTAGAA 
CAGCCTGCCA ACCTGCTTCG GATGTTCTTT GATGCTCTAT ATGATGAGGA CGTGGTGAAG 
GAAGACGCCT TCTACAGCTG GGAGAGCAGC AAGGATCCTG CTGAACAGCA GGGCAAGGGT 
GTGGCCCTTA AATCTGTCAC AGCATTCTTC AATTGGCTTC GTGAGGCTGA GGACGAGGAG 
TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_017316919.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172408.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu51990
Clone ID Related Accession (Same CDS sequence)	XM_030249025.1 , XM_017316919.1 , XM_006521943.1
Accession Version	XM_017316919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172408.1
CDS	654..5339
Translation	MNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_017316919.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316919.1

ORF Insert Sequence:

ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521943.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522006.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu51990
Clone ID Related Accession (Same CDS sequence)	XM_030249025.1 , XM_017316919.1 , XM_006521943.1
Accession Version	XM_006521943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522006.1
CDS	275..4960
Translation	MNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521943.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521943.1

ORF Insert Sequence:

ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT 
GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG 
TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT 
AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA 
GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC 
AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC 
CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG 
GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA 
GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG 
TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC 
AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG 
GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA 
CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA 
TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA 
CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA 
TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT 
CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG 
AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT 
CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG 
AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG 
GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA 
GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT 
AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC 
AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC 
CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC 
ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA 
CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA 
GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG 
CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC 
ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA 
CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC 
AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG 
TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA 
GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA 
ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA 
GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA 
GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA 
ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC 
TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG 
GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC 
CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA 
GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT 
CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC 
CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT 
GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG 
GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA 
GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC 
AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG 
GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC 
AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG 
CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT 
CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG 
GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA 
CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA 
CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG 
TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC 
CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC 
AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC 
CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT 
GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA 
AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC 
TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT 
GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT 
CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG 
GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG 
AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC 
AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC 
TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521944.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522007.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X13, mRNA.

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CloneID	OMu51991
Clone ID Related Accession (Same CDS sequence)	XM_006521944.3 , XM_030249026.1
Accession Version	XM_006521944.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521944.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522007.1
CDS	275..4939
Translation	MNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521944.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521944.3

ORF Insert Sequence:

ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA 
GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC 
TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT 
GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC 
ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT 
TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT 
CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA 
GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC 
ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT 
CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA 
GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA 
ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC 
CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT 
CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG 
GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC 
GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA 
GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG 
AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG 
GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG 
CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT 
GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT 
CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC 
TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG 
GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC 
CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC 
CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG 
CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT 
GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA 
GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC 
AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG 
GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT 
GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC 
ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG 
TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA 
AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA 
GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT 
GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG 
CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA 
GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG 
GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA 
CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT 
GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG 
CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT 
GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC 
CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC 
CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA 
GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT 
GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT 
AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT 
GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA 
AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC 
CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT 
CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT 
AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC 
CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG 
CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT 
GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT 
GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT 
ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG 
AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC 
ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT 
CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG 
GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG 
CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC 
AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC 
TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG 
CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT 
GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT 
GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC 
AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC 
AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521945.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522008.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X15, mRNA.

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CloneID	OMu51992
Clone ID Related Accession (Same CDS sequence)	XM_006521945.1
Accession Version	XM_006521945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG 
CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA 
GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT 
TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA 
GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC 
TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG 
CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG 
CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT 
TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC 
CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG 
ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC 
GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT 
TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC 
AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT 
CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG 
CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT 
GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT 
GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT 
GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT 
GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG 
GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT 
GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT 
CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG 
CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC 
ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT 
GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC 
ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA 
ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC 
ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT 
GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG CAGGCTGGCC TGGGACCCAG GCGCTCTCAG 
CAGGGCCCAC GAAAGGAAAC TCGCAAGATC ATTTCCTCAG TGATAATGAC TGAAGACATA 
AAACTAAATA AAGCAGAGAA GGCTTGGAAA CCCAGTAGCA AACGGACAGC AGCTGATAAG 
GATCGAGGGG AAGAGGATGC TGATGGAAGC AAGACCCAGG ACCTGTTCCG CAGGGTACGC 
TCCATCCTGA ATAAGCTGAC ACCCCAGATG TTTCAGCAGC TGATGAAGCA GGTGACACAG 
CTGGCCATTG ACACGGAGGA ACGCCTCAAA GGAGTTATTG ACCTCATCTT TGAGAAAGCC 
ATTTCAGAGC CCAACTTCTC TGTGGCTTAT GCCAACATGT GCCGCTGCCT CATGGCGCTG 
AAAGTGCCCA CTACAGAAAA GCCAACAGTA ACTGTGAATT TTCGAAAACT GTTGTTAAAT 
CGGTGCCAGA AGGAATTTGA GAAAGACAAA GATGATGATG AAGTTTTTGA AAAAAAGCAA 
AAAGAGATGG ATGAAGCTGC TACGGCAGAA GAACGGGGAC GCCTGAAAGA AGAGCTAGAA 
GAAGCCCGGG ACATAGCTAG GCGGCGCTCT TTAGGGAATA TCAAGTTTAT TGGAGAGTTA 
TTCAAGTTGA AGATGTTAAC AGAAGCAATA ATGCATGACT GTGTGGTCAA GCTACTTAAG 
AACCATGATG AGGAGTCCCT GGAATGCCTC TGCCGCCTCC TCACCACTAT AGGCAAAGAC 
CTGGACTTTG CAAAAGCCAA GCCTCGAATG GATCAGTATT TCAACCAAAT GGAAAAAATC 
ATTAAAGAAA AGAAGACCTC ATCTCGCATC CGTTTTATGC TGCAGGATGT ACTCGATCTG 
CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG GATCAGGGTC CTAAGACTAT TGATCAAATT 
CACAAGGAGG CTGAGATGGA AGAGCACCGA GAACATATTA AAGTACAGCA GCTCATGGCC 
AAGGGCAGCG ACAAACGTCG GGGTGGCCCT CCAGGCCCGC CTATCAACCG TGGCCTTCCA 
CTTGTAGATG ATGGTGGCTG GAATACAGTC CCCATTAGCA AAGGCAGTCG CCCCATTGAC 
ACCTCACGGC TCACCAAGAT CACAAAGCCT GGTTCCATTG ATTCTAACAA CCAGCTTTTT 
GCACCTGGAG GACGACTGAG TTGGGGCAAG GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC 
TCAGACACAG CATCGGAAGC TACTCGTCCA GCTACCTTGA ATCGCTTTTC TGCTCTTCAA 
CAAACATTAC CCGCAGAGAA CACAGATAAC AGACGTGTTG TACAGAGGAG TAGCTTGAGC 
CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGAC TAGAGCGGAG TGAGCGGGGA 
GGTGACCGTG GGGACCGACT TGATCGTGCC AGAACACCTG CCACCAAGCG AAGTTTTAGC 
AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG CCATCCCAGC CTGAGGGACT CCGCAAGGCA 
GCTAGCCTCA CAGAGGATCG TGGTCGGGAT CCTGTGAAGC GGGAAGCCAC TCTACCTCCA 
GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG GATGAGGTGG AGAAGAAATC TAAGGCCATC 
ATTGAGGAAT ATCTCCATCT CAATGACATG AAGGAGGCAG TACAGTGTGT CCAGGAGCTG 
GCTTCACCCT CCTTGCTCTT CATCTTCGTA CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT 
AGTACCATTG CTCGTGAGCA TATGGGGCGA CTACTGCACC AACTGCTCTG TGCGGGGCAC 
CTCTCTACTG CCCAGTACTA TCAAGGGCTG TATGAAACAC TAGAATTGGC TGAGGACATG 
GAAATTGATA TCCCTCATGT ATGGCTTTAC CTGGCAGAAC TGATTACACC TATTCTCCAG 
GAAGATGGGG TGCCCATGGG AGAGCTCTTC AGGGAAATTA CGAAGCCTCT GAGACCCATG 
GGCAAAGCTA CTTCTTTATT GCTGGAGATC CTGGGTCTCT TATGCAAGAG CATGGGTCCC 
AAAAAGGTGG GGATGCTGTG GCGAGAGGCT GGGCTGAGCT GGAGGGAATT TCTAGCAGAG 
GGCCAAGACG TTGGCTCATT TGTGGCTGAA AAGAAGGTGG AATATACCTT GGGAGAAGAA 
TCTGAAGCTC CTGGCCAGAG GACACTTGCC TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG 
CTGCTGAAGG ACGGCGGCAG TAATCAGCGT GTGTTCGACT GGATAGACGC CAACCTAAAT 
GAGCAGCAGA TAGCATCCAA TACATTAGTT CGAGCCCTCA TGACAACTGT CTGTTATTCT 
GCAATTATCT TTGAGACTCC TCTCCGAGTG GATGTTCAAG TGTTGAAAGT GCGAGCAAGA 
CTGCTGCAGA AATACCTGTG TGATGAGCAG AAGGAGCTAC AAGCACTCTA TGCTCTCCAG 
GCCCTTGTAG TGACCTTAGA ACAGCCTGCC AACCTGCTTC GGATGTTCTT TGATGCTCTA 
TATGATGAGG ACGTGGTGAA GGAAGACGCC TTCTACAGCT GGGAGAGCAG CAAGGATCCT 
GCTGAACAGC AGGGCAAGGG TGTGGCCCTT AAATCTGTCA CAGCATTCTT CAATTGGCTT 
CGTGAGGCTG AGGACGAGGA GTCTGATCAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522008.1
CDS	218..4873
Translation	MNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521945.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521945.1

ORF Insert Sequence:

ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG 
CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA 
GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT 
TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA 
GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC 
TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG 
CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG 
CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT 
TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC 
CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG 
ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC 
GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT 
TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC 
AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT 
CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG 
CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT 
GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT 
GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT 
GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT 
GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG 
GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT 
GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT 
CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG 
CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC 
ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT 
GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC 
ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA 
ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC 
ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT 
GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG CAGGCTGGCC TGGGACCCAG GCGCTCTCAG 
CAGGGCCCAC GAAAGGAAAC TCGCAAGATC ATTTCCTCAG TGATAATGAC TGAAGACATA 
AAACTAAATA AAGCAGAGAA GGCTTGGAAA CCCAGTAGCA AACGGACAGC AGCTGATAAG 
GATCGAGGGG AAGAGGATGC TGATGGAAGC AAGACCCAGG ACCTGTTCCG CAGGGTACGC 
TCCATCCTGA ATAAGCTGAC ACCCCAGATG TTTCAGCAGC TGATGAAGCA GGTGACACAG 
CTGGCCATTG ACACGGAGGA ACGCCTCAAA GGAGTTATTG ACCTCATCTT TGAGAAAGCC 
ATTTCAGAGC CCAACTTCTC TGTGGCTTAT GCCAACATGT GCCGCTGCCT CATGGCGCTG 
AAAGTGCCCA CTACAGAAAA GCCAACAGTA ACTGTGAATT TTCGAAAACT GTTGTTAAAT 
CGGTGCCAGA AGGAATTTGA GAAAGACAAA GATGATGATG AAGTTTTTGA AAAAAAGCAA 
AAAGAGATGG ATGAAGCTGC TACGGCAGAA GAACGGGGAC GCCTGAAAGA AGAGCTAGAA 
GAAGCCCGGG ACATAGCTAG GCGGCGCTCT TTAGGGAATA TCAAGTTTAT TGGAGAGTTA 
TTCAAGTTGA AGATGTTAAC AGAAGCAATA ATGCATGACT GTGTGGTCAA GCTACTTAAG 
AACCATGATG AGGAGTCCCT GGAATGCCTC TGCCGCCTCC TCACCACTAT AGGCAAAGAC 
CTGGACTTTG CAAAAGCCAA GCCTCGAATG GATCAGTATT TCAACCAAAT GGAAAAAATC 
ATTAAAGAAA AGAAGACCTC ATCTCGCATC CGTTTTATGC TGCAGGATGT ACTCGATCTG 
CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG GATCAGGGTC CTAAGACTAT TGATCAAATT 
CACAAGGAGG CTGAGATGGA AGAGCACCGA GAACATATTA AAGTACAGCA GCTCATGGCC 
AAGGGCAGCG ACAAACGTCG GGGTGGCCCT CCAGGCCCGC CTATCAACCG TGGCCTTCCA 
CTTGTAGATG ATGGTGGCTG GAATACAGTC CCCATTAGCA AAGGCAGTCG CCCCATTGAC 
ACCTCACGGC TCACCAAGAT CACAAAGCCT GGTTCCATTG ATTCTAACAA CCAGCTTTTT 
GCACCTGGAG GACGACTGAG TTGGGGCAAG GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC 
TCAGACACAG CATCGGAAGC TACTCGTCCA GCTACCTTGA ATCGCTTTTC TGCTCTTCAA 
CAAACATTAC CCGCAGAGAA CACAGATAAC AGACGTGTTG TACAGAGGAG TAGCTTGAGC 
CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGAC TAGAGCGGAG TGAGCGGGGA 
GGTGACCGTG GGGACCGACT TGATCGTGCC AGAACACCTG CCACCAAGCG AAGTTTTAGC 
AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG CCATCCCAGC CTGAGGGACT CCGCAAGGCA 
GCTAGCCTCA CAGAGGATCG TGGTCGGGAT CCTGTGAAGC GGGAAGCCAC TCTACCTCCA 
GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG GATGAGGTGG AGAAGAAATC TAAGGCCATC 
ATTGAGGAAT ATCTCCATCT CAATGACATG AAGGAGGCAG TACAGTGTGT CCAGGAGCTG 
GCTTCACCCT CCTTGCTCTT CATCTTCGTA CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT 
AGTACCATTG CTCGTGAGCA TATGGGGCGA CTACTGCACC AACTGCTCTG TGCGGGGCAC 
CTCTCTACTG CCCAGTACTA TCAAGGGCTG TATGAAACAC TAGAATTGGC TGAGGACATG 
GAAATTGATA TCCCTCATGT ATGGCTTTAC CTGGCAGAAC TGATTACACC TATTCTCCAG 
GAAGATGGGG TGCCCATGGG AGAGCTCTTC AGGGAAATTA CGAAGCCTCT GAGACCCATG 
GGCAAAGCTA CTTCTTTATT GCTGGAGATC CTGGGTCTCT TATGCAAGAG CATGGGTCCC 
AAAAAGGTGG GGATGCTGTG GCGAGAGGCT GGGCTGAGCT GGAGGGAATT TCTAGCAGAG 
GGCCAAGACG TTGGCTCATT TGTGGCTGAA AAGAAGGTGG AATATACCTT GGGAGAAGAA 
TCTGAAGCTC CTGGCCAGAG GACACTTGCC TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG 
CTGCTGAAGG ACGGCGGCAG TAATCAGCGT GTGTTCGACT GGATAGACGC CAACCTAAAT 
GAGCAGCAGA TAGCATCCAA TACATTAGTT CGAGCCCTCA TGACAACTGT CTGTTATTCT 
GCAATTATCT TTGAGACTCC TCTCCGAGTG GATGTTCAAG TGTTGAAAGT GCGAGCAAGA 
CTGCTGCAGA AATACCTGTG TGATGAGCAG AAGGAGCTAC AAGCACTCTA TGCTCTCCAG 
GCCCTTGTAG TGACCTTAGA ACAGCCTGCC AACCTGCTTC GGATGTTCTT TGATGCTCTA 
TATGATGAGG ACGTGGTGAA GGAAGACGCC TTCTACAGCT GGGAGAGCAG CAAGGATCCT 
GCTGAACAGC AGGGCAAGGG TGTGGCCCTT AAATCTGTCA CAGCATTCTT CAATTGGCTT 
CGTGAGGCTG AGGACGAGGA GTCTGATCAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_006521946.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522009.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu51993
Clone ID Related Accession (Same CDS sequence)	XM_006521946.1
Accession Version	XM_006521946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATCC CTTCCCAGAT CTCCTACTCA GCCTCCCAAG GAGCCTACTA TATCCCTGGA 
CAGGGGCGTT CCACATATGT TGTCCCAACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA 
GGCTTCTATC CAGGTGCAAG CCCTACCGAG TTTGGGACCT ATGCTGGAGC CTATTACCCA 
GCCCAAGGTG TGCAGCAGTT TCCTGCTAGT GTGGCTCCTG CCCCAGTTTT GATGAACCAG 
CCACCCCAGA TTGCTCCTAA GAGGGAACGG AAAACTATCC GAATTCGAGA CCCAAACCAA 
GGAGGGAAGG ATATCACAGA AGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACT 
CCTCCCCAGA CGGGAGGCAG TCTGGAGCCT CAACCCAATG GGGAGTCGCC TCAGGTTGCT 
GTCATTATCC GGCCAGATGA CCGGTCGCAG GGAGCAGCCA TTGGGGGGCG GCCAGGACTG 
CCTGGCCCAG AGCATAGCCC TGGCACAGAA TCTCAGCCTT CGTCGCCTTC TCCAACCCCA 
TCACCACCCC CAATTTTGGA GCCGGGGTCT GAGTCTAATC TTGGAGTCCT CTCTATTCCT 
GGGGACACTA TGACAACAGG GATGATACCA ATGTCTGTAG AAGAATCGAC CCCTATCTCT 
TGTGAAACTG GGGAGCCGTA TTGCCTCTCT CCAGAACCCA CTCTTGCCGA ACCCATACTG 
GAAGTAGAAG TGACACTCAG CAAACCCATT CCAGAATCTG AGTTCTCTTC CAGTCCTCTC 
CAGGTTTCCA CGGCCCTGGT GCCTCACAAG GTTGAAACTC ATGAGCCCAA TGGCGTGATC 
CCATCTGAGG ATCTGGAACC AGAGGTGGAG TCAAGCACAG AGCCAGCTCC TCCCCCTCTA 
TCACCTTGTG CTTCTGAATC GCTCGTGCCC ATTGCTCCAA CTGCCCAGCC TGAGGAACTG 
CTCAACGGAG CCCCCTCACC ACCAGCTGTG GATTTAAGCC CAGTCAGTGA GCCAGAGGAA 
CAGGCCAAGA AGGTTTCATC AGCAGCACTG GCCAGCATTC TCTCTCCTGC TCCACCTGTG 
GCTCCTTCAG ATACTTCTCC TGCTCAGGAG GAAGAAATGG AAGAAGATGA CGATGACGAA 
GAAGGTGGAG AAGCTGAGAG TGAGAAGGGA GGAGAGGACG TCCCCCTTGA CAGTACTCCT 
GTCCCAGCCC AGCTGTCTCA GAATTTAGAG GTGGCAGCAG CTACCCAGGT GGCAGTGTCT 
GTGCCAAAGA GGAGACGGAA AATTAAAGAG CTAAATAAGA AGGAGGCTGT AGGAGACCTT 
CTAGATGCCT TCAAGGAGGT GGACCCAGCA GTACCAGAGG TAGAGAATCA GCCTCCCACT 
GGCAGCAACC CAAGCCCAGA GTCTGAGGGC AGCATGGTGC CCACACAGCC TGAGGAAACA 
GAAGAAACCT GGGACTCTAA GGAAGACAAA ATTCATAATG CTGAGAACAT CCAGCCTGGG 
GAGCAGAAGT ATGAGTACAA GTCAGATCAG TGGAAGCCTC TAAACCTTGA AGAAAAGAAG 
CGTTATGACA GAGAATTCCT GCTGGGCTTT CAGTTCATCT TTGCCAGCAT GCAGAAGCCA 
GAAGGATTGC CCCATATCAC TGATGTGGTG CTGGACAAGG CCAATAAAAC ACCACTTCGG 
CAACTGGATC CCTCCAGACT ACCTGGCATA AACTGTGGCC CAGATTTCAC TCCATCTTTT 
GCCAATCTTG GCCGACCAGC CCTCAGCAAC CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG 
GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG GGACCCAGGC GCTCTCAGCA GGGCCCACGA 
AAGGAAACTC GCAAGATCAT TTCCTCAGTG ATAATGACTG AAGACATAAA ACTAAATAAA 
GCAGAGAAGG CTTGGAAACC CAGTAGCAAA CGGACAGCAG CTGATAAGGA TCGAGGGGAA 
GAGGATGCTG ATGGAAGCAA GACCCAGGAC CTGTTCCGCA GGGTACGCTC CATCCTGAAT 
AAGCTGACAC CCCAGATGTT TCAGCAGCTG ATGAAGCAGG TGACACAGCT GGCCATTGAC 
ACGGAGGAAC GCCTCAAAGG AGTTATTGAC CTCATCTTTG AGAAAGCCAT TTCAGAGCCC 
AACTTCTCTG TGGCTTATGC CAACATGTGC CGCTGCCTCA TGGCGCTGAA AGTGCCCACT 
ACAGAAAAGC CAACAGTAAC TGTGAATTTT CGAAAACTGT TGTTAAATCG GTGCCAGAAG 
GAATTTGAGA AAGACAAAGA TGATGATGAA GTTTTTGAAA AAAAGCAAAA AGAGATGGAT 
GAAGCTGCTA CGGCAGAAGA ACGGGGACGC CTGAAAGAAG AGCTAGAAGA AGCCCGGGAC 
ATAGCTAGGC GGCGCTCTTT AGGGAATATC AAGTTTATTG GAGAGTTATT CAAGTTGAAG 
ATGTTAACAG AAGCAATAAT GCATGACTGT GTGGTCAAGC TACTTAAGAA CCATGATGAG 
GAGTCCCTGG AATGCCTCTG CCGCCTCCTC ACCACTATAG GCAAAGACCT GGACTTTGCA 
AAAGCCAAGC CTCGAATGGA TCAGTATTTC AACCAAATGG AAAAAATCAT TAAAGAAAAG 
AAGACCTCAT CTCGCATCCG TTTTATGCTG CAGGATGTAC TCGATCTGCG GCAGAGTAAT 
TGGGTGCCAC GCCGAGGGGA TCAGGGTCCT AAGACTATTG ATCAAATTCA CAAGGAGGCT 
GAGATGGAAG AGCACCGAGA ACATATTAAA GTACAGCAGC TCATGGCCAA GGGCAGCGAC 
AAACGTCGGG GTGGCCCTCC AGGCCCGCCT ATCAACCGTG GCCTTCCACT TGTAGATGAT 
GGTGGCTGGA ATACAGTCCC CATTAGCAAA GGCAGTCGCC CCATTGACAC CTCACGGCTC 
ACCAAGATCA CAAAGCCTGG TTCCATTGAT TCTAACAACC AGCTTTTTGC ACCTGGAGGA 
CGACTGAGTT GGGGCAAGGG CAGCAGTGGG GGCTCAGGAG CCAAGCCCTC AGACACAGCA 
TCGGAAGCTA CTCGTCCAGC TACCTTGAAT CGCTTTTCTG CTCTTCAACA AACATTACCC 
GCAGAGAACA CAGATAACAG ACGTGTTGTA CAGAGGAGTA GCTTGAGCCG GGAACGAGGT 
GAGAAAGCTG GGGACCGGGG AGACCGACTA GAGCGGAGTG AGCGGGGAGG TGACCGTGGG 
GACCGACTTG ATCGTGCCAG AACACCTGCC ACCAAGCGAA GTTTTAGCAA AGAAGTGGAG 
GAGCGAAGTA GAGAGCGGCC ATCCCAGCCT GAGGGACTCC GCAAGGCAGC TAGCCTCACA 
GAGGATCGTG GTCGGGATCC TGTGAAGCGG GAAGCCACTC TACCTCCAGT GAGCCCTCCA 
AAGGCTGCGC TGTCTGTGGA TGAGGTGGAG AAGAAATCTA AGGCCATCAT TGAGGAATAT 
CTCCATCTCA ATGACATGAA GGAGGCAGTA CAGTGTGTCC AGGAGCTGGC TTCACCCTCC 
TTGCTCTTCA TCTTCGTACG GCTTGGTATC GAGTCCACGC TGGAGCGTAG TACCATTGCT 
CGTGAGCATA TGGGGCGACT ACTGCACCAA CTGCTCTGTG CGGGGCACCT CTCTACTGCC 
CAGTACTATC AAGGGCTGTA TGAAACACTA GAATTGGCTG AGGACATGGA AATTGATATC 
CCTCATGTAT GGCTTTACCT GGCAGAACTG ATTACACCTA TTCTCCAGGA AGATGGGGTG 
CCCATGGGAG AGCTCTTCAG GGAAATTACG AAGCCTCTGA GACCCATGGG CAAAGCTACT 
TCTTTATTGC TGGAGATCCT GGGTCTCTTA TGCAAGAGCA TGGGTCCCAA AAAGGTGGGG 
ATGCTGTGGC GAGAGGCTGG GCTGAGCTGG AGGGAATTTC TAGCAGAGGG CCAAGACGTT 
GGCTCATTTG TGGCTGAAAA GAAGGTGGAA TATACCTTGG GAGAAGAATC TGAAGCTCCT 
GGCCAGAGGA CACTTGCCTT TGAGGAGCTT CGTAGGCAGC TAGAGAAGCT GCTGAAGGAC 
GGCGGCAGTA ATCAGCGTGT GTTCGACTGG ATAGACGCCA ACCTAAATGA GCAGCAGATA 
GCATCCAATA CATTAGTTCG AGCCCTCATG ACAACTGTCT GTTATTCTGC AATTATCTTT 
GAGACTCCTC TCCGAGTGGA TGTTCAAGTG TTGAAAGTGC GAGCAAGACT GCTGCAGAAA 
TACCTGTGTG ATGAGCAGAA GGAGCTACAA GCACTCTATG CTCTCCAGGC CCTTGTAGTG 
ACCTTAGAAC AGCCTGCCAA CCTGCTTCGG ATGTTCTTTG ATGCTCTATA TGATGAGGAC 
GTGGTGAAGG AAGACGCCTT CTACAGCTGG GAGAGCAGCA AGGATCCTGC TGAACAGCAG 
GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCATTCTTCA ATTGGCTTCG TGAGGCTGAG 
GACGAGGAGT CTGATCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522009.1
CDS	272..4795
Translation	MMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_006521946.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521946.1

ORF Insert Sequence:

ATGATGATCC CTTCCCAGAT CTCCTACTCA GCCTCCCAAG GAGCCTACTA TATCCCTGGA 
CAGGGGCGTT CCACATATGT TGTCCCAACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA 
GGCTTCTATC CAGGTGCAAG CCCTACCGAG TTTGGGACCT ATGCTGGAGC CTATTACCCA 
GCCCAAGGTG TGCAGCAGTT TCCTGCTAGT GTGGCTCCTG CCCCAGTTTT GATGAACCAG 
CCACCCCAGA TTGCTCCTAA GAGGGAACGG AAAACTATCC GAATTCGAGA CCCAAACCAA 
GGAGGGAAGG ATATCACAGA AGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACT 
CCTCCCCAGA CGGGAGGCAG TCTGGAGCCT CAACCCAATG GGGAGTCGCC TCAGGTTGCT 
GTCATTATCC GGCCAGATGA CCGGTCGCAG GGAGCAGCCA TTGGGGGGCG GCCAGGACTG 
CCTGGCCCAG AGCATAGCCC TGGCACAGAA TCTCAGCCTT CGTCGCCTTC TCCAACCCCA 
TCACCACCCC CAATTTTGGA GCCGGGGTCT GAGTCTAATC TTGGAGTCCT CTCTATTCCT 
GGGGACACTA TGACAACAGG GATGATACCA ATGTCTGTAG AAGAATCGAC CCCTATCTCT 
TGTGAAACTG GGGAGCCGTA TTGCCTCTCT CCAGAACCCA CTCTTGCCGA ACCCATACTG 
GAAGTAGAAG TGACACTCAG CAAACCCATT CCAGAATCTG AGTTCTCTTC CAGTCCTCTC 
CAGGTTTCCA CGGCCCTGGT GCCTCACAAG GTTGAAACTC ATGAGCCCAA TGGCGTGATC 
CCATCTGAGG ATCTGGAACC AGAGGTGGAG TCAAGCACAG AGCCAGCTCC TCCCCCTCTA 
TCACCTTGTG CTTCTGAATC GCTCGTGCCC ATTGCTCCAA CTGCCCAGCC TGAGGAACTG 
CTCAACGGAG CCCCCTCACC ACCAGCTGTG GATTTAAGCC CAGTCAGTGA GCCAGAGGAA 
CAGGCCAAGA AGGTTTCATC AGCAGCACTG GCCAGCATTC TCTCTCCTGC TCCACCTGTG 
GCTCCTTCAG ATACTTCTCC TGCTCAGGAG GAAGAAATGG AAGAAGATGA CGATGACGAA 
GAAGGTGGAG AAGCTGAGAG TGAGAAGGGA GGAGAGGACG TCCCCCTTGA CAGTACTCCT 
GTCCCAGCCC AGCTGTCTCA GAATTTAGAG GTGGCAGCAG CTACCCAGGT GGCAGTGTCT 
GTGCCAAAGA GGAGACGGAA AATTAAAGAG CTAAATAAGA AGGAGGCTGT AGGAGACCTT 
CTAGATGCCT TCAAGGAGGT GGACCCAGCA GTACCAGAGG TAGAGAATCA GCCTCCCACT 
GGCAGCAACC CAAGCCCAGA GTCTGAGGGC AGCATGGTGC CCACACAGCC TGAGGAAACA 
GAAGAAACCT GGGACTCTAA GGAAGACAAA ATTCATAATG CTGAGAACAT CCAGCCTGGG 
GAGCAGAAGT ATGAGTACAA GTCAGATCAG TGGAAGCCTC TAAACCTTGA AGAAAAGAAG 
CGTTATGACA GAGAATTCCT GCTGGGCTTT CAGTTCATCT TTGCCAGCAT GCAGAAGCCA 
GAAGGATTGC CCCATATCAC TGATGTGGTG CTGGACAAGG CCAATAAAAC ACCACTTCGG 
CAACTGGATC CCTCCAGACT ACCTGGCATA AACTGTGGCC CAGATTTCAC TCCATCTTTT 
GCCAATCTTG GCCGACCAGC CCTCAGCAAC CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG 
GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG GGACCCAGGC GCTCTCAGCA GGGCCCACGA 
AAGGAAACTC GCAAGATCAT TTCCTCAGTG ATAATGACTG AAGACATAAA ACTAAATAAA 
GCAGAGAAGG CTTGGAAACC CAGTAGCAAA CGGACAGCAG CTGATAAGGA TCGAGGGGAA 
GAGGATGCTG ATGGAAGCAA GACCCAGGAC CTGTTCCGCA GGGTACGCTC CATCCTGAAT 
AAGCTGACAC CCCAGATGTT TCAGCAGCTG ATGAAGCAGG TGACACAGCT GGCCATTGAC 
ACGGAGGAAC GCCTCAAAGG AGTTATTGAC CTCATCTTTG AGAAAGCCAT TTCAGAGCCC 
AACTTCTCTG TGGCTTATGC CAACATGTGC CGCTGCCTCA TGGCGCTGAA AGTGCCCACT 
ACAGAAAAGC CAACAGTAAC TGTGAATTTT CGAAAACTGT TGTTAAATCG GTGCCAGAAG 
GAATTTGAGA AAGACAAAGA TGATGATGAA GTTTTTGAAA AAAAGCAAAA AGAGATGGAT 
GAAGCTGCTA CGGCAGAAGA ACGGGGACGC CTGAAAGAAG AGCTAGAAGA AGCCCGGGAC 
ATAGCTAGGC GGCGCTCTTT AGGGAATATC AAGTTTATTG GAGAGTTATT CAAGTTGAAG 
ATGTTAACAG AAGCAATAAT GCATGACTGT GTGGTCAAGC TACTTAAGAA CCATGATGAG 
GAGTCCCTGG AATGCCTCTG CCGCCTCCTC ACCACTATAG GCAAAGACCT GGACTTTGCA 
AAAGCCAAGC CTCGAATGGA TCAGTATTTC AACCAAATGG AAAAAATCAT TAAAGAAAAG 
AAGACCTCAT CTCGCATCCG TTTTATGCTG CAGGATGTAC TCGATCTGCG GCAGAGTAAT 
TGGGTGCCAC GCCGAGGGGA TCAGGGTCCT AAGACTATTG ATCAAATTCA CAAGGAGGCT 
GAGATGGAAG AGCACCGAGA ACATATTAAA GTACAGCAGC TCATGGCCAA GGGCAGCGAC 
AAACGTCGGG GTGGCCCTCC AGGCCCGCCT ATCAACCGTG GCCTTCCACT TGTAGATGAT 
GGTGGCTGGA ATACAGTCCC CATTAGCAAA GGCAGTCGCC CCATTGACAC CTCACGGCTC 
ACCAAGATCA CAAAGCCTGG TTCCATTGAT TCTAACAACC AGCTTTTTGC ACCTGGAGGA 
CGACTGAGTT GGGGCAAGGG CAGCAGTGGG GGCTCAGGAG CCAAGCCCTC AGACACAGCA 
TCGGAAGCTA CTCGTCCAGC TACCTTGAAT CGCTTTTCTG CTCTTCAACA AACATTACCC 
GCAGAGAACA CAGATAACAG ACGTGTTGTA CAGAGGAGTA GCTTGAGCCG GGAACGAGGT 
GAGAAAGCTG GGGACCGGGG AGACCGACTA GAGCGGAGTG AGCGGGGAGG TGACCGTGGG 
GACCGACTTG ATCGTGCCAG AACACCTGCC ACCAAGCGAA GTTTTAGCAA AGAAGTGGAG 
GAGCGAAGTA GAGAGCGGCC ATCCCAGCCT GAGGGACTCC GCAAGGCAGC TAGCCTCACA 
GAGGATCGTG GTCGGGATCC TGTGAAGCGG GAAGCCACTC TACCTCCAGT GAGCCCTCCA 
AAGGCTGCGC TGTCTGTGGA TGAGGTGGAG AAGAAATCTA AGGCCATCAT TGAGGAATAT 
CTCCATCTCA ATGACATGAA GGAGGCAGTA CAGTGTGTCC AGGAGCTGGC TTCACCCTCC 
TTGCTCTTCA TCTTCGTACG GCTTGGTATC GAGTCCACGC TGGAGCGTAG TACCATTGCT 
CGTGAGCATA TGGGGCGACT ACTGCACCAA CTGCTCTGTG CGGGGCACCT CTCTACTGCC 
CAGTACTATC AAGGGCTGTA TGAAACACTA GAATTGGCTG AGGACATGGA AATTGATATC 
CCTCATGTAT GGCTTTACCT GGCAGAACTG ATTACACCTA TTCTCCAGGA AGATGGGGTG 
CCCATGGGAG AGCTCTTCAG GGAAATTACG AAGCCTCTGA GACCCATGGG CAAAGCTACT 
TCTTTATTGC TGGAGATCCT GGGTCTCTTA TGCAAGAGCA TGGGTCCCAA AAAGGTGGGG 
ATGCTGTGGC GAGAGGCTGG GCTGAGCTGG AGGGAATTTC TAGCAGAGGG CCAAGACGTT 
GGCTCATTTG TGGCTGAAAA GAAGGTGGAA TATACCTTGG GAGAAGAATC TGAAGCTCCT 
GGCCAGAGGA CACTTGCCTT TGAGGAGCTT CGTAGGCAGC TAGAGAAGCT GCTGAAGGAC 
GGCGGCAGTA ATCAGCGTGT GTTCGACTGG ATAGACGCCA ACCTAAATGA GCAGCAGATA 
GCATCCAATA CATTAGTTCG AGCCCTCATG ACAACTGTCT GTTATTCTGC AATTATCTTT 
GAGACTCCTC TCCGAGTGGA TGTTCAAGTG TTGAAAGTGC GAGCAAGACT GCTGCAGAAA 
TACCTGTGTG ATGAGCAGAA GGAGCTACAA GCACTCTATG CTCTCCAGGC CCTTGTAGTG 
ACCTTAGAAC AGCCTGCCAA CCTGCTTCGG ATGTTCTTTG ATGCTCTATA TGATGAGGAC 
GTGGTGAAGG AAGACGCCTT CTACAGCTGG GAGAGCAGCA AGGATCCTGC TGAACAGCAG 
GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCATTCTTCA ATTGGCTTCG TGAGGCTGAG 
GACGAGGAGT CTGATCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4g1 ( XM_011245861.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244163.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu72646
Clone ID Related Accession (Same CDS sequence)	XM_011245861.1
Accession Version	XM_011245861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG 
CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA 
GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT 
TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA 
GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC 
TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG 
CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG 
CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT 
TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC 
CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG 
ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC 
GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT 
TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC 
AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT 
CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG 
CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT 
GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT 
GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT 
GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT 
GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG 
GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT 
GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT 
CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG 
CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC 
ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT 
GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC 
ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA 
ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC 
ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT 
GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG CAGGCTGGCC TGGGACCCAG GCGCTCTCAG 
CAGGGCCCAC GAAAGGAAAC TCGCAAGATC ATTTCCTCAG TGATAATGAC TGAAGACATA 
AAACTAAATA AAGCAGAGAA GGCTTGGAAA CCCAGTAGCA AACGGACAGC AGCTGATAAG 
GATCGAGGGG AAGAGGATGC TGATGGAAGC AAGACCCAGG ACCTGTTCCG CAGGGTACGC 
TCCATCCTGA ATAAGCTGAC ACCCCAGATG TTTCAGCAGC TGATGAAGCA GGTGACACAG 
CTGGCCATTG ACACGGAGGA ACGCCTCAAA GGAGTTATTG ACCTCATCTT TGAGAAAGCC 
ATTTCAGAGC CCAACTTCTC TGTGGCTTAT GCCAACATGT GCCGCTGCCT CATGGCGCTG 
AAAGTGCCCA CTACAGAAAA GCCAACAGTA ACTGTGAATT TTCGAAAACT GTTGTTAAAT 
CGGTGCCAGA AGGAATTTGA GAAAGACAAA GATGATGATG AAGTTTTTGA AAAAAAGCAA 
AAAGAGATGG ATGAAGCTGC TACGGCAGAA GAACGGGGAC GCCTGAAAGA AGAGCTAGAA 
GAAGCCCGGG ACATAGCTAG GCGGCGCTCT TTAGGGAATA TCAAGTTTAT TGGAGAGTTA 
TTCAAGTTGA AGATGTTAAC AGAAGCAATA ATGCATGACT GTGTGGTCAA GCTACTTAAG 
AACCATGATG AGGAGTCCCT GGAATGCCTC TGCCGCCTCC TCACCACTAT AGGCAAAGAC 
CTGGACTTTG CAAAAGCCAA GCCTCGAATG GATCAGTATT TCAACCAAAT GGAAAAAATC 
ATTAAAGAAA AGAAGACCTC ATCTCGCATC CGTTTTATGC TGCAGGATGT ACTCGATCTG 
CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG GATCAGGGTC CTAAGACTAT TGATCAAATT 
CACAAGGAGG CTGAGATGGA AGAGCACCGA GAACATATTA AAGTACAGCA GCTCATGGCC 
AAGGGCAGCG ACAAACGTCG GGGTGGCCCT CCAGGCCCGC CTATCAACCG TGGCCTTCCA 
CTTGTAGATG ATGGTGGCTG GAATACAGTC CCCATTAGCA AAGGCAGTCG CCCCATTGAC 
ACCTCACGGC TCACCAAGAT CACAAAGCCT GGTTCCATTG ATTCTAACAA CCAGCTTTTT 
GCACCTGGAG GACGACTGAG TTGGGGCAAG GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC 
TCAGACACAG CATCGGAAGC TACTCGTCCA GCTACCTTGA ATCGCTTTTC TGCTCTTCAA 
CAAACATTAC CCGCAGAGAA CACAGATAAC AGACGTGTTG TACAGAGGAG TAGCTTGAGC 
CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGAC TAGAGCGGAG TGAGCGGGGA 
GGTGACCGTG GGGACCGACT TGATCGTGCC AGAACACCTG CCACCAAGCG AAGTTTTAGC 
AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG CCATCCCAGC CTGAGGGACT CCGCAAGGCA 
GCTAGCCTCA CAGAGGATCG TGGTCGGGAT CCTGTGAAGC GGGAAGCCAC TCTACCTCCA 
GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG GATGAGGTGG AGAAGAAATC TAAGGCCATC 
ATTGAGGAAT ATCTCCATCT CAATGACATG AAGGAGGCAG TACAGTGTGT CCAGGAGCTG 
GCTTCACCCT CCTTGCTCTT CATCTTCGTA CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT 
AGTACCATTG CTCGTGAGCA TATGGGGCGA CTACTGCACC AACTGCTCTG TGCGGGGCAC 
CTCTCTACTG CCCAGTACTA TCAAGGGCTG TATGAAACAC TAGAATTGGC TGAGGACATG 
GAAATTGATA TCCCTCATGT ATGGCTTTAC CTGGCAGAAC TGATTACACC TATTCTCCAG 
GAAGATGGGG TGCCCATGGG AGAGCTCTTC AGGGAAATTA CGAAGCCTCT GAGACCCATG 
GGCAAAGCTA CTTCTTTATT GCTGGAGATC CTGGGTCTCT TATGCAAGAG CATGGGTCCC 
AAAAAGGTGG GGATGCTGTG GCGAGAGGCT GGGCTGAGCT GGAGGGAATT TCTAGCAGAG 
GGCCAAGACG TTGGCTCATT TGTGGCTGAA AAGAAGGTGG AATATACCTT GGGAGAAGAA 
TCTGAAGCTC CTGGCCAGAG GACACTTGCC TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG 
CTGCTGAAGG ACGGCGGCAG TAATCAGCGT GTGTTCGACT GGATAGACGC CAACCTAAAT 
GAGCAGCAGA TAGCATCCAA TACATTAGTT CGAGCCCTCA TGACAACTGT CTGTTATTCT 
GCAATTATCT TTGAGACTCC TCTCCGAGTG GATGTTCAAG TGTTGAAAGT GCGAGCAAGA 
CTGCTGCAGA AATACCTGTG TGATGAGCAG AAGGAGCTAC AAGCACTCTA TGCTCTCCAG 
GCCCTTGTAG TGACCTTAGA ACAGCCTGCC AACCTGCTTC GGATGTTCTT TGATGCTCTA 
TATGATGAGG ACGTGGTGAA GGAAGACGCC TTCTACAGCT GGGAGAGCAG CAAGGATCCT 
GCTGAACAGC AGGGCAAGGG TGTGGCCCTT AAATCTGTCA CAGCATTCTT CAATTGGCTT 
CGTGAGGCTG AGGACGAGGA GTCTGATCAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244163.1
CDS	249..5024
Translation	MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN

Target ORF information:

RefSeq Version	XM_011245861.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245861.1

ORF Insert Sequence:

ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT 
CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG 
CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA 
GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT 
TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA 
GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC 
TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG 
CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG 
CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT 
TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC 
CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG 
ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC 
GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT 
TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC 
AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT 
CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG 
CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT 
GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT 
GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT 
GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT 
GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG 
GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT 
GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT 
CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG 
CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC 
ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT 
GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC 
ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA 
ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC 
ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT 
GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG CAGGCTGGCC TGGGACCCAG GCGCTCTCAG 
CAGGGCCCAC GAAAGGAAAC TCGCAAGATC ATTTCCTCAG TGATAATGAC TGAAGACATA 
AAACTAAATA AAGCAGAGAA GGCTTGGAAA CCCAGTAGCA AACGGACAGC AGCTGATAAG 
GATCGAGGGG AAGAGGATGC TGATGGAAGC AAGACCCAGG ACCTGTTCCG CAGGGTACGC 
TCCATCCTGA ATAAGCTGAC ACCCCAGATG TTTCAGCAGC TGATGAAGCA GGTGACACAG 
CTGGCCATTG ACACGGAGGA ACGCCTCAAA GGAGTTATTG ACCTCATCTT TGAGAAAGCC 
ATTTCAGAGC CCAACTTCTC TGTGGCTTAT GCCAACATGT GCCGCTGCCT CATGGCGCTG 
AAAGTGCCCA CTACAGAAAA GCCAACAGTA ACTGTGAATT TTCGAAAACT GTTGTTAAAT 
CGGTGCCAGA AGGAATTTGA GAAAGACAAA GATGATGATG AAGTTTTTGA AAAAAAGCAA 
AAAGAGATGG ATGAAGCTGC TACGGCAGAA GAACGGGGAC GCCTGAAAGA AGAGCTAGAA 
GAAGCCCGGG ACATAGCTAG GCGGCGCTCT TTAGGGAATA TCAAGTTTAT TGGAGAGTTA 
TTCAAGTTGA AGATGTTAAC AGAAGCAATA ATGCATGACT GTGTGGTCAA GCTACTTAAG 
AACCATGATG AGGAGTCCCT GGAATGCCTC TGCCGCCTCC TCACCACTAT AGGCAAAGAC 
CTGGACTTTG CAAAAGCCAA GCCTCGAATG GATCAGTATT TCAACCAAAT GGAAAAAATC 
ATTAAAGAAA AGAAGACCTC ATCTCGCATC CGTTTTATGC TGCAGGATGT ACTCGATCTG 
CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG GATCAGGGTC CTAAGACTAT TGATCAAATT 
CACAAGGAGG CTGAGATGGA AGAGCACCGA GAACATATTA AAGTACAGCA GCTCATGGCC 
AAGGGCAGCG ACAAACGTCG GGGTGGCCCT CCAGGCCCGC CTATCAACCG TGGCCTTCCA 
CTTGTAGATG ATGGTGGCTG GAATACAGTC CCCATTAGCA AAGGCAGTCG CCCCATTGAC 
ACCTCACGGC TCACCAAGAT CACAAAGCCT GGTTCCATTG ATTCTAACAA CCAGCTTTTT 
GCACCTGGAG GACGACTGAG TTGGGGCAAG GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC 
TCAGACACAG CATCGGAAGC TACTCGTCCA GCTACCTTGA ATCGCTTTTC TGCTCTTCAA 
CAAACATTAC CCGCAGAGAA CACAGATAAC AGACGTGTTG TACAGAGGAG TAGCTTGAGC 
CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGAC TAGAGCGGAG TGAGCGGGGA 
GGTGACCGTG GGGACCGACT TGATCGTGCC AGAACACCTG CCACCAAGCG AAGTTTTAGC 
AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG CCATCCCAGC CTGAGGGACT CCGCAAGGCA 
GCTAGCCTCA CAGAGGATCG TGGTCGGGAT CCTGTGAAGC GGGAAGCCAC TCTACCTCCA 
GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG GATGAGGTGG AGAAGAAATC TAAGGCCATC 
ATTGAGGAAT ATCTCCATCT CAATGACATG AAGGAGGCAG TACAGTGTGT CCAGGAGCTG 
GCTTCACCCT CCTTGCTCTT CATCTTCGTA CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT 
AGTACCATTG CTCGTGAGCA TATGGGGCGA CTACTGCACC AACTGCTCTG TGCGGGGCAC 
CTCTCTACTG CCCAGTACTA TCAAGGGCTG TATGAAACAC TAGAATTGGC TGAGGACATG 
GAAATTGATA TCCCTCATGT ATGGCTTTAC CTGGCAGAAC TGATTACACC TATTCTCCAG 
GAAGATGGGG TGCCCATGGG AGAGCTCTTC AGGGAAATTA CGAAGCCTCT GAGACCCATG 
GGCAAAGCTA CTTCTTTATT GCTGGAGATC CTGGGTCTCT TATGCAAGAG CATGGGTCCC 
AAAAAGGTGG GGATGCTGTG GCGAGAGGCT GGGCTGAGCT GGAGGGAATT TCTAGCAGAG 
GGCCAAGACG TTGGCTCATT TGTGGCTGAA AAGAAGGTGG AATATACCTT GGGAGAAGAA 
TCTGAAGCTC CTGGCCAGAG GACACTTGCC TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG 
CTGCTGAAGG ACGGCGGCAG TAATCAGCGT GTGTTCGACT GGATAGACGC CAACCTAAAT 
GAGCAGCAGA TAGCATCCAA TACATTAGTT CGAGCCCTCA TGACAACTGT CTGTTATTCT 
GCAATTATCT TTGAGACTCC TCTCCGAGTG GATGTTCAAG TGTTGAAAGT GCGAGCAAGA 
CTGCTGCAGA AATACCTGTG TGATGAGCAG AAGGAGCTAC AAGCACTCTA TGCTCTCCAG 
GCCCTTGTAG TGACCTTAGA ACAGCCTGCC AACCTGCTTC GGATGTTCTT TGATGCTCTA 
TATGATGAGG ACGTGGTGAA GGAAGACGCC TTCTACAGCT GGGAGAGCAG CAAGGATCCT 
GCTGAACAGC AGGGCAAGGG TGTGGCCCTT AAATCTGTCA CAGCATTCTT CAATTGGCTT 
CGTGAGGCTG AGGACGAGGA GTCTGATCAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential Requirements for eIF4E Dose in Normal Development and Cancer.
Cell162(1)59-71(2015 Jul)
Truitt ML,Conn CS,Shi Z,Pang X,Tokuyasu T,Coady AM,Seo Y,Barna M,Ruggero D

Parkinson's disease genes VPS35 and EIF4G1 interact genetically and converge on ?-synuclein.
Neuron85(1)76-87(2015 Jan)
Dhungel N,Eleuteri S,Li LB,Kramer NJ,Chartron JW,Spencer B,Kosberg K,Fields JA,Stafa K,Adame A,Lashuel H,Frydman J,Shen K,Masliah E,Gitler AD

An eIF4E1/4E-T complex determines the genesis of neurons from precursors by translationally repressing a proneurogenic transcription program.
Neuron84(4)723-39(2014 Nov)
Yang G,Smibert CA,Kaplan DR,Miller FD

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Eif4g1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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