Sptlc2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptlc2
Entrez Gene ID	20773
Full Name	serine palmitoyltransferase, long chain base subunit 2
Synonyms	LCB2,LCB2a,Spt2
General protein information	Preferred Names serine palmitoyltransferase, long chain base subunit 2 Names serine palmitoyltransferase 2 LCB 2 SPT 2 long chain base biosynthesis protein 2 long chain base biosynthesis protein 2a serine-palmitoyl-CoA transferase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 D2
Summary	This gene encodes a long chain base subunit of serine palmitoyltransferase. The enzyme, serine palmitoyltransferase, consists of two different subunits, and is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. A mutant allele of this gene in mice is used as a model for the human disease 'Susceptibilty to Psoriasis 1'. Mutations in the human gene are associated with hereditary sensory neuropathy type I. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_011479.4	NP_035609.1	serine palmitoyltransferase 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPTLC2	XP_510095.3
Caenorhabditis elegans (roundworm)	sptl-2	NP_505065.1
Magnaporthe oryzae (rice blast fungus)	MGG_05197	XP_003712740.1
Homo sapiens (human)	SPTLC2	NP_004854.1
Rattus norvegicus (Norway rat)	Sptlc2	NP_001032174.1
Gallus gallus (chicken)	SPTLC2	NP_001006483.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007941	XP_317536.4
Schizosaccharomyces pombe (fission yeast)	lcb2	XP_001713103.1
Arabidopsis thaliana (thale cress)	LCB2	NP_197756.1
Oryza sativa (rice)	Os01g0928700	NP_001045278.1
Oryza sativa (rice)	Os01g0928800	NP_001045279.1
Mus musculus (house mouse)	Sptlc2	NP_035609.1
Kluyveromyces lactis	KLLA0D02134g	XP_453163.1
Oryza sativa (rice)	Os11g0516000	NP_001067972.1
Danio rerio (zebrafish)	sptlc2a	NP_001018455.1
Saccharomyces cerevisiae (baker's yeast)	LCB2	NP_010347.1
Neurospora crassa	NCU00447	XP_956766.1
	LOC707625	XP_001096042.2
Canis lupus familiaris (dog)	SPTLC2	XP_537524.3
Bos taurus (cattle)	SPTLC2	NP_001092551.1
Drosophila melanogaster (fruit fly)	lace	NP_476614.1
Eremothecium gossypii	AGOS_AGR127C	NP_986793.2
Arabidopsis thaliana (thale cress)	SPT1	NP_190447.1
Xenopus tropicalis (tropical clawed frog)	sptlc2	XP_002938915.1

Gene Ontology
Bibliography

Related articles in PubMed

Adipocyte-Specific Deficiency of De Novo Sphingolipid Biosynthesis Leads to Lipodystrophy and Insulin Resistance.
Lee SY, Lee HY, Song JH, Kim GT, Jeon S, Song YJ, Lee JS, Hur JH, Oh HH, Park SY, Shim SM, Yoo HJ, Lee BC, Jiang XC, Choi CS, Park TS
Diabetes66(10)2596-2609(2017 10)

Adipocyte Ceramides Regulate Subcutaneous Adipose Browning, Inflammation, and Metabolism.
Chaurasia B, Kaddai VA, Lancaster GI, Henstridge DC, Sriram S, Galam DL, Gopalan V, Prakash KN, Velan SS, Bulchand S, Tsong TJ, Wang M, Siddique MM, Yuguang G, Sigmundsson K, Mellet NA, Weir JM, Meikle PJ, Bin M Yassin MS, Shabbir A, Shayman JA, Hirabayashi Y, Shiow ST, Sugii S, Summers SA
Cell metabolism24(6)820-834(2016 Dec)

Macrophage-specific de Novo Synthesis of Ceramide Is Dispensable for Inflammasome-driven Inflammation and Insulin Resistance in Obesity.
Camell CD, Nguyen KY, Jurczak MJ, Christian BE, Shulman GI, Shadel GS, Dixit VD
The Journal of biological chemistry290(49)29402-13(2015 Dec)

DJ-1-mediated upregulation of serine palmitoyltransferase 2 controls vascular neointima via S1P autocrine.
Lee KP, Won KJ, Lee DH, Lee DY, Jung SH, Baek S, Park TS, Kim B
International journal of cardiology191220-2(2015 Jul)

ORMDL/serine palmitoyltransferase stoichiometry determines effects of ORMDL3 expression on sphingolipid biosynthesis.
Siow D, Sunkara M, Dunn TM, Morris AJ, Wattenberg B
Journal of lipid research56(4)898-908(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of SPT2 was stronger in vascular smooth muscle cells and neointima of carotid arteries from knock-out mice compared to wild-type.
Title: DJ-1-mediated upregulation of serine palmitoyltransferase 2 controls vascular neointima via S1P autocrine.

Data suggest that overexpression of serine palmitoyltransferase (Sptlc1 and Sptlc2, serine palmitoyltransferase long chain base subunit 1/2) to elevate de novo sphingolipid biosynthesis induces autophagy in the liver.
Title: Autophagy regulates sphingolipid levels in the liver.

SPTLC2 knockout mice showed decreased ceramide levels in the epidermis, which impaired water-holding capacity and barrier function.
Title: Barrier abnormality due to ceramide deficiency leads to psoriasiform inflammation in a mouse model.

Ldlr gene knockout mice with myeloid cell-specific Sptlc2 haploinsufficiency exhibited significantly less atherosclerosis than controls.
Title: Myeloid cell-specific serine palmitoyltransferase subunit 2 haploinsufficiency reduces murine atherosclerosis.

results suggest that Sptlc2-mediated de novo ceramide synthesis is an essential source of C18:0 and very long chain, but not of shorter chain, ceramides in the heart
Title: Cardiomyocyte specific deficiency of serine palmitoyltransferase subunit 2 reduces ceramide but leads to cardiac dysfunction.

The following Sptlc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptlc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21698	NM_011479.4 Latest version!	Mus musculus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptlc2 ( NM_011479.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035609.1 Mus musculus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.

Price & Availability↑

CloneID	OMu21698
Clone ID Related Accession (Same CDS sequence)	NM_011479.4
Accession Version	NM_011479.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	serine palmitoyltransferase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC120540.6, BY789607.1, AK044917.1, AK037908.1 and CF747116.1. On Sep 16, 2015 this sequence version replaced NM_011479.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK044917.1, SRR1660819.51550.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCGG AGCCCGGAGG CTGCTGCTGC CGCCGCCCGA TGCGGGCGAA CGGCTGCGTC 
AAGAACGGGG AAGTGAGGAA CGGGTACTTG AGGAGCAGCA CCGCCACCGT CGCGGCTGCC 
GGCCAGATTC ATCATGTAAC AGAAAATGGA GGACTGTACA AAAGACCGTT TAATGAAGCT 
TTTGAAGAAA CACCCATGCT GGTTGCTGTG CTCACATATG TGGGCTATGG CGTACTCACC 
CTCTTTGGAT ATCTTCGAGA TTTCTTGAGG CATTGGAGAA TTGAAAAGTG CCACCATGCA 
ACAGAAAGAG AAGAACAAAA GGACTTTGTG TCCTTGTATC AGGATTTTGA AAACTTCTAT 
ACAAGGAACC TCTACATGAG AATCAGAGAC AACTGGAATC GACCTATCTG TAGTGTGCCT 
GGAGCCAAGG TGGATATCAT GGAGAGAAAA TCTCATGACT ATAACTGGTC ATTCAAGTAC 
ACAGGGAATA TAATTAAAGG TGTAATAAAC ATGGGTTCCT ACAACTATCT TGGATTTGCG 
AGGAACACTG GATCATGTCA GGAAGCAGCT GCTGAAGTCC TCAAGGAGTA TGGAGCAGGG 
GTGTGCAGCA CTCGTCAGGA AATTGGAAAC CTGGACAAGC ATGAAGAACT AGAGAAATTG 
GTAGCAAGGT TCTTAGGTGT GGAAGCTGCT ATGACCTATG GCATGGGATT TGCAACAAAT 
TCAATGAACA TTCCTGCTCT TGTTGGCAAA GGTTGCCTGA TTCTGAGTGA TGAGCTGAAC 
CATGCGTCAC TGGTTCTAGG AGCCAGACTG TCAGGAGCAA CCATTCGAAT CTTCAAACAC 
AACAATATGC AAAGCTTAGA GAAGCTTTTA AAAGATGCCA TTGTTTATGG TCAGCCTCGG 
ACAAGAAGAC CCTGGAAGAA AATCTTAATC CTTGTGGAAG GCATATATAG TATGGAGGGG 
TCTATTGTTC GCCTTCCTGA AGTGATTGCT CTCAAGAAGA AATACAAGGC ATACTTGTAT 
CTGGATGAGG CTCACAGCAT TGGGGCTCTT GGCCCTTCAG GGCGAGGCGT GGTAGATTAC 
TTTGGCCTGG ATCCTGAGGA TGTAGATGTT ATGATGGGAA CATTCACAAA GAGCTTCGGT 
GCTTCAGGAG GATACATCGG AGGCAAGAAG GAGCTGATAG ACTACCTGCG CACACATTCT 
CACAGTGCTG TGTATGCCAC GTCGATGTCA CCGCCTGTGA TGGAACAGAT TATCACCTCC 
ATGAAGTGCA TCATGGGGCA GGATGGCACC AGTCTTGGCA AAGAATGTAT ACAGCAGTTG 
GCTGAGAACA CCAGGTATTT CAGGAGACGC CTGAAGGAAA TGGGGTTCAT CATCTATGGC 
AATGAAGACT CCCCGGTGGT GCCTTTGATG CTCTACATGC CGGCCAAAAT TGGCGCCTTT 
GGAAGAGAGA TGCTGAAGCG GAACATTGGT GTAGTTGTGG TGGGATTTCC TGCTACCCCG 
ATCATTGAGT CCAGAGCCAG ATTTTGCCTG TCAGCAGCTC ATACCAAAGA AATACTTGAC 
ACTGCTTTGA AGGAGATAGA TGAAGTTGGG GATCTGCTGC AGCTAAAGTA CTCTCGCCAC 
CGGCTGGTGC CTCTACTGGA CAGGCCCTTT GATGAGACTA CCTATGAAGA GACAGAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035609.1
CDS	185..1867
Translation	MRPEPGGCCCRRPMRANGCVKNGEVRNGYLRSSTATVAAAGQIHHVTENGGLYKRPFNEAFEETPMLVAVLTYVGYGVLTLFGYLRDFLRHWRIEKCHHATEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVPGAKVDIMERKSHDYNWSFKYTGNIIKGVINMGSYNYLGFARNTGSCQEAAAEVLKEYGAGVCSTRQEIGNLDKHEELEKLVARFLGVEAAMTYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPSGRGVVDYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSMSPPVMEQIITSMKCIMGQDGTSLGKECIQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED

Target ORF information:

RefSeq Version	NM_011479.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011479.4

ORF Insert Sequence:

ATGCGGCCGG AGCCCGGAGG CTGCTGCTGC CGCCGCCCGA TGCGGGCGAA CGGCTGCGTC 
AAGAACGGGG AAGTGAGGAA CGGGTACTTG AGGAGCAGCA CCGCCACCGT CGCGGCTGCC 
GGCCAGATTC ATCATGTAAC AGAAAATGGA GGACTGTACA AAAGACCGTT TAATGAAGCT 
TTTGAAGAAA CACCCATGCT GGTTGCTGTG CTCACATATG TGGGCTATGG CGTACTCACC 
CTCTTTGGAT ATCTTCGAGA TTTCTTGAGG CATTGGAGAA TTGAAAAGTG CCACCATGCA 
ACAGAAAGAG AAGAACAAAA GGACTTTGTG TCCTTGTATC AGGATTTTGA AAACTTCTAT 
ACAAGGAACC TCTACATGAG AATCAGAGAC AACTGGAATC GACCTATCTG TAGTGTGCCT 
GGAGCCAAGG TGGATATCAT GGAGAGAAAA TCTCATGACT ATAACTGGTC ATTCAAGTAC 
ACAGGGAATA TAATTAAAGG TGTAATAAAC ATGGGTTCCT ACAACTATCT TGGATTTGCG 
AGGAACACTG GATCATGTCA GGAAGCAGCT GCTGAAGTCC TCAAGGAGTA TGGAGCAGGG 
GTGTGCAGCA CTCGTCAGGA AATTGGAAAC CTGGACAAGC ATGAAGAACT AGAGAAATTG 
GTAGCAAGGT TCTTAGGTGT GGAAGCTGCT ATGACCTATG GCATGGGATT TGCAACAAAT 
TCAATGAACA TTCCTGCTCT TGTTGGCAAA GGTTGCCTGA TTCTGAGTGA TGAGCTGAAC 
CATGCGTCAC TGGTTCTAGG AGCCAGACTG TCAGGAGCAA CCATTCGAAT CTTCAAACAC 
AACAATATGC AAAGCTTAGA GAAGCTTTTA AAAGATGCCA TTGTTTATGG TCAGCCTCGG 
ACAAGAAGAC CCTGGAAGAA AATCTTAATC CTTGTGGAAG GCATATATAG TATGGAGGGG 
TCTATTGTTC GCCTTCCTGA AGTGATTGCT CTCAAGAAGA AATACAAGGC ATACTTGTAT 
CTGGATGAGG CTCACAGCAT TGGGGCTCTT GGCCCTTCAG GGCGAGGCGT GGTAGATTAC 
TTTGGCCTGG ATCCTGAGGA TGTAGATGTT ATGATGGGAA CATTCACAAA GAGCTTCGGT 
GCTTCAGGAG GATACATCGG AGGCAAGAAG GAGCTGATAG ACTACCTGCG CACACATTCT 
CACAGTGCTG TGTATGCCAC GTCGATGTCA CCGCCTGTGA TGGAACAGAT TATCACCTCC 
ATGAAGTGCA TCATGGGGCA GGATGGCACC AGTCTTGGCA AAGAATGTAT ACAGCAGTTG 
GCTGAGAACA CCAGGTATTT CAGGAGACGC CTGAAGGAAA TGGGGTTCAT CATCTATGGC 
AATGAAGACT CCCCGGTGGT GCCTTTGATG CTCTACATGC CGGCCAAAAT TGGCGCCTTT 
GGAAGAGAGA TGCTGAAGCG GAACATTGGT GTAGTTGTGG TGGGATTTCC TGCTACCCCG 
ATCATTGAGT CCAGAGCCAG ATTTTGCCTG TCAGCAGCTC ATACCAAAGA AATACTTGAC 
ACTGCTTTGA AGGAGATAGA TGAAGTTGGG GATCTGCTGC AGCTAAAGTA CTCTCGCCAC 
CGGCTGGTGC CTCTACTGGA CAGGCCCTTT GATGAGACTA CCTATGAAGA GACAGAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Adipocyte-Specific Deficiency of De Novo Sphingolipid Biosynthesis Leads to Lipodystrophy and Insulin Resistance.
Diabetes66(10)2596-2609(2017 10)
Lee SY,Lee HY,Song JH,Kim GT,Jeon S,Song YJ,Lee JS,Hur JH,Oh HH,Park SY,Shim SM,Yoo HJ,Lee BC,Jiang XC,Choi CS,Park TS

Adipocyte Ceramides Regulate Subcutaneous Adipose Browning, Inflammation, and Metabolism.
Cell metabolism24(6)820-834(2016 Dec)
Chaurasia B,Kaddai VA,Lancaster GI,Henstridge DC,Sriram S,Galam DL,Gopalan V,Prakash KN,Velan SS,Bulchand S,Tsong TJ,Wang M,Siddique MM,Yuguang G,Sigmundsson K,Mellet NA,Weir JM,Meikle PJ,Bin M Yassin MS,Shabbir A,Shayman JA,Hirabayashi Y,Shiow ST,Sugii S,Summers SA

Macrophage-specific de Novo Synthesis of Ceramide Is Dispensable for Inflammasome-driven Inflammation and Insulin Resistance in Obesity.
The Journal of biological chemistry290(49)29402-13(2015 Dec)
Camell CD,Nguyen KY,Jurczak MJ,Christian BE,Shulman GI,Shadel GS,Dixit VD

DJ-1-mediated upregulation of serine palmitoyltransferase 2 controls vascular neointima via S1P autocrine.
International journal of cardiology191220-2(2015 Jul)
Lee KP,Won KJ,Lee DH,Lee DY,Jung SH,Baek S,Park TS,Kim B

ORMDL/serine palmitoyltransferase stoichiometry determines effects of ORMDL3 expression on sphingolipid biosynthesis.
Journal of lipid research56(4)898-908(2015 Apr)
Siow D,Sunkara M,Dunn TM,Morris AJ,Wattenberg B

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Sptlc2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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