Spint2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spint2
Entrez Gene ID	20733
Full Name	serine protease inhibitor, Kunitz type 2
Synonyms	AL024025,C76321,HAI-2
General protein information	Preferred Names serine protease inhibitor, Kunitz type 2 Names kunitz-type protease inhibitor 2 hepatocyte growth factor activator inhibitor type 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017322072.1	XP_017177561.1	kunitz-type protease inhibitor 2 isoform X1
NM_011464.2	NP_035594.1	kunitz-type protease inhibitor 2 isoform a precursor
NM_001082548.1	NP_001076017.1	kunitz-type protease inhibitor 2 isoform b precursor
XM_017322072.2	XP_017177561.1	kunitz-type protease inhibitor 2 isoform X1

Pathways from BioSystems

Homologies

	SPINT2	XP_001106508.1
Mus musculus (house mouse)	Spint2	NP_035594.1
Xenopus tropicalis (tropical clawed frog)	spint2	NP_001039077.1
Bos taurus (cattle)	SPINT2	NP_001039436.1
Canis lupus familiaris (dog)	SPINT2	XP_853537.1
Rattus norvegicus (Norway rat)	Spint2	NP_001076018.1
Danio rerio (zebrafish)	spint2	NP_001070718.2
Homo sapiens (human)	SPINT2	NP_066925.1

Gene Ontology
Bibliography

Related articles in PubMed

Delineation of proteolytic and non-proteolytic functions of the membrane-anchored serine protease prostasin.
Szabo R, Lantsman T, Peters DE, Bugge TH
Development (Cambridge, England)143(15)2818-28(2016 08)

The protease inhibitor HAI-2, but not HAI-1, regulates matriptase activation and shedding through prostasin.
Friis S, Sales KU, Schafer JM, Vogel LK, Kataoka H, Bugge TH
The Journal of biological chemistry289(32)22319-32(2014 Aug)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation.
Szabo R, Uzzun Sales K, Kosa P, Shylo NA, Godiksen S, Hansen KK, Friis S, Gutkind JS, Vogel LK, Hummler E, Camerer E, Bugge TH
PLoS genetics8(8)e1002937(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HAI-1 regulates the activity of activated matriptase, whereas HAI-2 has an essential role in regulating prostasin-dependent matriptase zymogen activation.
Title: The protease inhibitor HAI-2, but not HAI-1, regulates matriptase activation and shedding through prostasin.

mutations in Prss8 restored placentation and normal development of HAI-1-deficient embryos and prevented early embryonic lethality, mid-gestation lethality due to placental labyrinth failure, and neural tube defects in HAI-2-deficient embryos.
Title: Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation.

HAI-1 and -2 are overexpressed in the liver in cholangiopathies with ductular reactions and are possibly involved in liver fibrosis and hepatic differentiation.
Title: Persistent elevation of hepatocyte growth factor activator inhibitors in cholangiopathies affects liver fibrosis and differentiation.

Inhibition of the transmembrane serine protease matriptase (encoded by St14) is an essential function of HAI2 during tissue morphogenesis.
Title: Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice.

Unlike HAI-1 and matriptase, HAI-2 expression is detected in non-epithelial cells of brain and lymph nodes, suggesting that HAI-2 may also be involved in inhibition of serine proteases other than matriptase
Title: Potent inhibition and global co-localization implicate the transmembrane Kunitz-type serine protease inhibitor hepatocyte growth factor activator inhibitor-2 in the regulation of epithelial matriptase activity.

The following Spint2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spint2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23460	NM_001082548.1 Latest version!	Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu23470	NM_011464.2 Latest version!	Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu81977	XM_017322072.2 Latest version!	Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu81977	XM_017322072.1	Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spint2 ( NM_001082548.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001076017.1 Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23460
Clone ID Related Accession (Same CDS sequence)	NM_001082548.1
Accession Version	NM_001082548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	kunitz-type protease inhibitor 2 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB196296.1, AI227451.1, AK007792.1 and BQ174562.1. Transcript Variant: This variant (2) lacks an alternate exon, in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003431.1, BI410932.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGC TTTGTGAGCT GCGGCGGGGC CGGGCGCTCC TCGCCTTGGT AGCTTCGCTG 
CTCCTCTCCG GGGCCCAGGT GGCCAGCCGA GAACTAGACG TCCACGAGAA CACCACTGAT 
GACATGGCCA GGAACAGGAA TGGAGCCGAC TCTTCTGTCC TGAGTGTTCC CAGAAAGCAG 
AGTGCTGAGG ACCTGTCTGC TGAGATCTTC AACTATGAAG AATACTGTGT CCCGAAGGCC 
GTCACTGGGC CGTGCCGCGC AGCCTTCCCT CGCTGGTACT ATGACACTGA AAAGAATTCC 
TGTATCAGCT TCATCTACGG CGGGTGCAGA GGCAACAAGA ACAGTTACCT CTCCCAGGAA 
GCATGCATGC AGCACTGCTC TGGTAAGCAG ATGCATCCTT TCCTGACCCC GGGCCTAAAA 
GCGGTGATCC TGGTGGGGCT GTTCCTCATG GTTCTGATCC TCCTCCTGGG AACCTCTATG 
GTCTGCCTCA TCCGTGTGGT TCGCAGAAAG CAGGAGCGTG CGCTGCGCAC TGTTTGGAGC 
ACTGCGGATG ACAAGGAGCA GCTGGTGAAG AACACCTGTG TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076017.1
CDS	361..948
Translation	MAQLCELRRGRALLALVASLLLSGAQVASRELDVHENTTDDMARNRNGADSSVLSVPRKQSAEDLSAEIFNYEEYCVPKAVTGPCRAAFPRWYYDTEKNSCISFIYGGCRGNKNSYLSQEACMQHCSGKQMHPFLTPGLKAVILVGLFLMVLILLLGTSMVCLIRVVRRKQERALRTVWSTADDKEQLVKNTCVL

Target ORF information:

RefSeq Version	NM_001082548.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082548.1

ORF Insert Sequence:

ATGGCGCAGC TTTGTGAGCT GCGGCGGGGC CGGGCGCTCC TCGCCTTGGT AGCTTCGCTG 
CTCCTCTCCG GGGCCCAGGT GGCCAGCCGA GAACTAGACG TCCACGAGAA CACCACTGAT 
GACATGGCCA GGAACAGGAA TGGAGCCGAC TCTTCTGTCC TGAGTGTTCC CAGAAAGCAG 
AGTGCTGAGG ACCTGTCTGC TGAGATCTTC AACTATGAAG AATACTGTGT CCCGAAGGCC 
GTCACTGGGC CGTGCCGCGC AGCCTTCCCT CGCTGGTACT ATGACACTGA AAAGAATTCC 
TGTATCAGCT TCATCTACGG CGGGTGCAGA GGCAACAAGA ACAGTTACCT CTCCCAGGAA 
GCATGCATGC AGCACTGCTC TGGTAAGCAG ATGCATCCTT TCCTGACCCC GGGCCTAAAA 
GCGGTGATCC TGGTGGGGCT GTTCCTCATG GTTCTGATCC TCCTCCTGGG AACCTCTATG 
GTCTGCCTCA TCCGTGTGGT TCGCAGAAAG CAGGAGCGTG CGCTGCGCAC TGTTTGGAGC 
ACTGCGGATG ACAAGGAGCA GCTGGTGAAG AACACCTGTG TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spint2 ( NM_011464.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035594.1 Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23470
Clone ID Related Accession (Same CDS sequence)	NM_011464.2
Accession Version	NM_011464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	kunitz-type protease inhibitor 2 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB196296.1, AI227451.1, AK007792.1 and BQ174562.1. On Mar 9, 2007 this sequence version replaced NM_011464.1. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI415743.1, BG965694.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGC TTTGTGAGCT GCGGCGGGGC CGGGCGCTCC TCGCCTTGGT AGCTTCGCTG 
CTCCTCTCCG GGGCCCAGGT GGCCAGCCGA GAACTAGACG TCCACGAGTC TTGTGGAGTT 
TCGAAGGTAG TGGGGAAATG CCGGGCTTCC ATCCCCAGGT GGTGGTACAA TATCACTGAT 
GGGTCCTGCC AGCCATTTGT TTATGGAGGC TGTGAAGGCA ATGGCAATAA CTACCAGAGC 
AAGGAGGAGT GTCTTGACAA GTGTGCTGGT GTCACTGAGA ACACCACTGA TGACATGGCC 
AGGAACAGGA ATGGAGCCGA CTCTTCTGTC CTGAGTGTTC CCAGAAAGCA GAGTGCTGAG 
GACCTGTCTG CTGAGATCTT CAACTATGAA GAATACTGTG TCCCGAAGGC CGTCACTGGG 
CCGTGCCGCG CAGCCTTCCC TCGCTGGTAC TATGACACTG AAAAGAATTC CTGTATCAGC 
TTCATCTACG GCGGGTGCAG AGGCAACAAG AACAGTTACC TCTCCCAGGA AGCATGCATG 
CAGCACTGCT CTGGTAAGCA GATGCATCCT TTCCTGACCC CGGGCCTAAA AGCGGTGATC 
CTGGTGGGGC TGTTCCTCAT GGTTCTGATC CTCCTCCTGG GAACCTCTAT GGTCTGCCTC 
ATCCGTGTGG TTCGCAGAAA GCAGGAGCGT GCGCTGCGCA CTGTTTGGAG CACTGCGGAT 
GACAAGGAGC AGCTGGTGAA GAACACCTGT GTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035594.1
CDS	361..1119
Translation	MAQLCELRRGRALLALVASLLLSGAQVASRELDVHESCGVSKVVGKCRASIPRWWYNITDGSCQPFVYGGCEGNGNNYQSKEECLDKCAGVTENTTDDMARNRNGADSSVLSVPRKQSAEDLSAEIFNYEEYCVPKAVTGPCRAAFPRWYYDTEKNSCISFIYGGCRGNKNSYLSQEACMQHCSGKQMHPFLTPGLKAVILVGLFLMVLILLLGTSMVCLIRVVRRKQERALRTVWSTADDKEQLVKNTCVL

Target ORF information:

RefSeq Version	NM_011464.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011464.2

ORF Insert Sequence:

ATGGCGCAGC TTTGTGAGCT GCGGCGGGGC CGGGCGCTCC TCGCCTTGGT AGCTTCGCTG 
CTCCTCTCCG GGGCCCAGGT GGCCAGCCGA GAACTAGACG TCCACGAGTC TTGTGGAGTT 
TCGAAGGTAG TGGGGAAATG CCGGGCTTCC ATCCCCAGGT GGTGGTACAA TATCACTGAT 
GGGTCCTGCC AGCCATTTGT TTATGGAGGC TGTGAAGGCA ATGGCAATAA CTACCAGAGC 
AAGGAGGAGT GTCTTGACAA GTGTGCTGGT GTCACTGAGA ACACCACTGA TGACATGGCC 
AGGAACAGGA ATGGAGCCGA CTCTTCTGTC CTGAGTGTTC CCAGAAAGCA GAGTGCTGAG 
GACCTGTCTG CTGAGATCTT CAACTATGAA GAATACTGTG TCCCGAAGGC CGTCACTGGG 
CCGTGCCGCG CAGCCTTCCC TCGCTGGTAC TATGACACTG AAAAGAATTC CTGTATCAGC 
TTCATCTACG GCGGGTGCAG AGGCAACAAG AACAGTTACC TCTCCCAGGA AGCATGCATG 
CAGCACTGCT CTGGTAAGCA GATGCATCCT TTCCTGACCC CGGGCCTAAA AGCGGTGATC 
CTGGTGGGGC TGTTCCTCAT GGTTCTGATC CTCCTCCTGG GAACCTCTAT GGTCTGCCTC 
ATCCGTGTGG TTCGCAGAAA GCAGGAGCGT GCGCTGCGCA CTGTTTGGAG CACTGCGGAT 
GACAAGGAGC AGCTGGTGAA GAACACCTGT GTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spint2 ( XM_017322072.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177561.1 Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81977
Clone ID Related Accession (Same CDS sequence)	XM_017322072.1 , XM_017322072.2
Accession Version	XM_017322072.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kunitz-type protease inhibitor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC TTTGTGAGCT GCGGCGGGGC CGGGCGCTCC TCGCCTTGGT AGCTTCGCTG 
CTCCTCTCCG GGGCCCAGGT GGCCAGCCGA GAACTAGACG TCCACGAGTC TTGTGGAGTT 
TCGAAGGTAG TGGGGAAATG CCGGGCTTCC ATCCCCAGGT GGTGGTACAA TATCACTGAT 
GGGTCCTGCC AGCCATTTGT TTATGGAGGC TGTGAAGGCA ATGGCAATAA CTACCAGAGC 
AAGGAGGAGT GTCTTGACAA GTGTGCTGGT GTCACTGAGA ACACCACTGA TGACATGGCC 
AGGAACAGGA ATGGAGCCGA CTCTTCTGTC CTGAGTGTTT GCTTTGTAGA GCTGTCAGTA 
GCTGCCCTGT TCCTCTTTTA TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177561.1
CDS	450..836
Translation	MAQLCELRRGRALLALVASLLLSGAQVASRELDVHESCGVSKVVGKCRASIPRWWYNITDGSCQPFVYGGCEGNGNNYQSKEECLDKCAGVTENTTDDMARNRNGADSSVLSVCFVELSVAALFLFYA

Target ORF information:

RefSeq Version	XM_017322072.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322072.1

ORF Insert Sequence:

ATGGCGCAGC TTTGTGAGCT GCGGCGGGGC CGGGCGCTCC TCGCCTTGGT AGCTTCGCTG 
CTCCTCTCCG GGGCCCAGGT GGCCAGCCGA GAACTAGACG TCCACGAGTC TTGTGGAGTT 
TCGAAGGTAG TGGGGAAATG CCGGGCTTCC ATCCCCAGGT GGTGGTACAA TATCACTGAT 
GGGTCCTGCC AGCCATTTGT TTATGGAGGC TGTGAAGGCA ATGGCAATAA CTACCAGAGC 
AAGGAGGAGT GTCTTGACAA GTGTGCTGGT GTCACTGAGA ACACCACTGA TGACATGGCC 
AGGAACAGGA ATGGAGCCGA CTCTTCTGTC CTGAGTGTTT GCTTTGTAGA GCTGTCAGTA 
GCTGCCCTGT TCCTCTTTTA TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spint2 ( XM_017322072.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177561.1 Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81977
Clone ID Related Accession (Same CDS sequence)	XM_017322072.1 , XM_017322072.2
Accession Version	XM_017322072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kunitz-type protease inhibitor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017322072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC TTTGTGAGCT GCGGCGGGGC CGGGCGCTCC TCGCCTTGGT AGCTTCGCTG 
CTCCTCTCCG GGGCCCAGGT GGCCAGCCGA GAACTAGACG TCCACGAGTC TTGTGGAGTT 
TCGAAGGTAG TGGGGAAATG CCGGGCTTCC ATCCCCAGGT GGTGGTACAA TATCACTGAT 
GGGTCCTGCC AGCCATTTGT TTATGGAGGC TGTGAAGGCA ATGGCAATAA CTACCAGAGC 
AAGGAGGAGT GTCTTGACAA GTGTGCTGGT GTCACTGAGA ACACCACTGA TGACATGGCC 
AGGAACAGGA ATGGAGCCGA CTCTTCTGTC CTGAGTGTTT GCTTTGTAGA GCTGTCAGTA 
GCTGCCCTGT TCCTCTTTTA TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177561.1
CDS	443..829
Translation	MAQLCELRRGRALLALVASLLLSGAQVASRELDVHESCGVSKVVGKCRASIPRWWYNITDGSCQPFVYGGCEGNGNNYQSKEECLDKCAGVTENTTDDMARNRNGADSSVLSVCFVELSVAALFLFYA

Target ORF information:

RefSeq Version	XM_017322072.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322072.2

ORF Insert Sequence:

ATGGCGCAGC TTTGTGAGCT GCGGCGGGGC CGGGCGCTCC TCGCCTTGGT AGCTTCGCTG 
CTCCTCTCCG GGGCCCAGGT GGCCAGCCGA GAACTAGACG TCCACGAGTC TTGTGGAGTT 
TCGAAGGTAG TGGGGAAATG CCGGGCTTCC ATCCCCAGGT GGTGGTACAA TATCACTGAT 
GGGTCCTGCC AGCCATTTGT TTATGGAGGC TGTGAAGGCA ATGGCAATAA CTACCAGAGC 
AAGGAGGAGT GTCTTGACAA GTGTGCTGGT GTCACTGAGA ACACCACTGA TGACATGGCC 
AGGAACAGGA ATGGAGCCGA CTCTTCTGTC CTGAGTGTTT GCTTTGTAGA GCTGTCAGTA 
GCTGCCCTGT TCCTCTTTTA TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Delineation of proteolytic and non-proteolytic functions of the membrane-anchored serine protease prostasin.
Development (Cambridge, England)143(15)2818-28(2016 08)
Szabo R,Lantsman T,Peters DE,Bugge TH

The protease inhibitor HAI-2, but not HAI-1, regulates matriptase activation and shedding through prostasin.
The Journal of biological chemistry289(32)22319-32(2014 Aug)
Friis S,Sales KU,Schafer JM,Vogel LK,Kataoka H,Bugge TH

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation.
PLoS genetics8(8)e1002937(2012)
Szabo R,Uzzun Sales K,Kosa P,Shylo NA,Godiksen S,Hansen KK,Friis S,Gutkind JS,Vogel LK,Hummler E,Camerer E,Bugge TH

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Spint2 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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