Serpina3n cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Serpina3n
Entrez Gene ID	20716
Full Name	serine (or cysteine) peptidase inhibitor, clade A, member 3N
Synonyms	Spi2-2,Spi2.2,Spi2/eb.4
General protein information	Preferred Names serine (or cysteine) peptidase inhibitor, clade A, member 3N Names serine protease inhibitor A3N alpha-1 antiproteinase antitrypsin serine (or cysteine) proteinase inhibitor, clade A, member 3N serine protease inhibitor 2-2 serpin A3N
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 E

mRNA and Protein(s)

mRNA	Protein	Name
NM_009252.2	NP_033278.2	serine protease inhibitor A3N precursor
XM_011244046.3	XP_011242348.1	serine protease inhibitor A3N isoform X1
XM_011244046.2	XP_011242348.1	serine protease inhibitor A3N isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SERPINA3-7	NP_001012283.2
Bos taurus (cattle)	LOC784932	XP_002696874.1
Bos taurus (cattle)	SERPINA3	XP_005222351.1
Mus musculus (house mouse)	Serpina3n	NP_033278.2
Homo sapiens (human)	SERPINA3	NP_001076.2
Bos taurus (cattle)	SERPINA3-1	NP_777193.2
Bos taurus (cattle)	SERPINA3-3	NP_001033293.1
Mus musculus (house mouse)	Serpina3c	NP_032484.1
Pan troglodytes (chimpanzee)	SERPINA3	XP_510145.2
Mus musculus (house mouse)	Serpina3k	NP_035588.2
Mus musculus (house mouse)	Serpina3m	NP_033279.2
Rattus norvegicus (Norway rat)	Serpina3n	NP_113719.1
	SERPINA3	XP_001096947.2
Canis lupus familiaris (dog)	SERPINA3	XP_005623829.1
Bos taurus (cattle)	SERPINA3-8	NP_001075181.1

Gene Ontology
Bibliography

Related articles in PubMed

Trim33 Binds and Silences a Class of Young Endogenous Retroviruses in the Mouse Testis; a Novel Component of the Arms Race between Retrotransposons and the Host Genome.
Isbel L, Srivastava R, Oey H, Spurling A, Daxinger L, Puthalakath H, Whitelaw E
PLoS genetics11(12)e1005693(2015 Dec)

The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase.
Vicu?a L, Strochlic DE, Latremoliere A, Bali KK, Simonetti M, Husainie D, Prokosch S, Riva P, Griffin RS, Njoo C, Gehrig S, Mall MA, Arnold B, Devor M, Woolf CJ, Liberles SD, Costigan M, Kuner R
Nature medicine21(5)518-23(2015 May)

Serine protease inhibitors serpina1 and serpina3 are down-regulated in bone marrow during hematopoietic progenitor mobilization.
Winkler IG, Hendy J, Coughlin P, Horvath A, L?vesque JP
The Journal of experimental medicine201(7)1077-88(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase
Title: The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase.

Serpina3n accelerates wound healing through increase cell proliferation,vascularization, cell differentiation, and collagen deposition.
Title: Serpina3n accelerates tissue repair in a diabetic mouse model of delayed wound healing.

The genetically deleting Tff3 in mice altered down-regulated Pttg1 and up-regulated serpina3n expression, indicating a potential role of these factors during the development of presbyacusis.
Title: Altered expression of securin (Pttg1) and serpina3n in the auditory system of hearing-impaired Tff3-deficient mice.

discovery of a new inhibitor of granzyme B and a new mechanism used by Sertoli cells for immunoprotection
Title: Identification of a novel human granzyme B inhibitor secreted by cultured sertoli cells.

The following Serpina3n gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Serpina3n cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03798	NM_009252.2 Latest version!	Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu03798	XM_011244046.3 Latest version!	Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu03798	XM_011244046.2	Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Serpina3n ( NM_009252.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033278.2 Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n), mRNA.

Price & Availability↑

CloneID	OMu03798
Clone ID Related Accession (Same CDS sequence)	NM_009252.2 , XM_011244046.3 , XM_011244046.2
Accession Version	NM_009252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor A3N precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117194.3. On Mar 14, 2007 this sequence version replaced NM_009252.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013651.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTCA TTGCAGCTCT GGGGCTCTTG ATGGCTGGGA TCTGCCCTGC TGTCCTCTGC 
TTCCCAGATG GCACGTTGGG AATGGATGCT GCAGTCCAAG AAGACCATGA CAATGGGACA 
CAACTGGACA GTCTCACATT GGCCTCCATC AACACTGACT TTGCCTTCAG CCTCTACAAG 
GAGCTGGTTT TGAAGAATCC AGATAAAAAT ATTGTCTTCT CCCCACTTAG CATCTCAGCG 
GCCTTGGCCG TCATGTCCCT GGGAGCAAAG GGCAACACCC TGGAAGAGAT TCTAGAAGGT 
CTCAAGTTCA ATCTTACAGA GACCTCTGAG GCAGACATCC ACCAGGGCTT TGGGCACCTC 
CTACAGAGGC TCAACCAGCC AAAGGACCAG GTACAGATCA GCACGGGTAG TGCCCTGTTT 
ATTGAAAAGC GCCAGCAGAT CCTGACAGAA TTCCAGGAGA AGGCAAGGGC TCTGTACCAG 
GCTGAGGCCT TCACAGCAGA CTTCCAGCAG CCTCGTCAGG CCAAAAAGCT CATCAATGAC 
TATGTGAGGA AACAGACCCA GGGGATGATC AAGGAACTGG TCTCAGACCT GGATAAAAGG 
ACATTGATGG TGCTGGTGAA TTATATCTAC TTTAAAGCCA AATGGAAGGT GCCCTTTGAC 
CCTCTTGACA CGTTCAAGTC TGAGTTCTAC GCGGGCAAGA GGAGGCCCGT GATAGTGCCC 
ATGATGAGCA TGGAGGACCT GACCACACCC TACTTCCGAG ATGAGGAGCT TTTCTGCACT 
GTGGTGGAGC TGAAGTACAC AGGAAATGCC AGTGCCATGT TCATCCTCCC TGACCAGGGC 
AAGATGCAGC AGGTGGAAGC CAGCTTGCAA CCAGAGACCC TGAGGAAGTG GAAGAATTCT 
CTGAAACCCA GGATGATAGA TGAGCTCCAC CTGCCCAAGT TCTCCATCTC CACCGACTAC 
AGCCTGGAGG ATGTCCTTTC AAAGCTGGGC ATCAGGGAAG TCTTCTCCAC ACAGGCTGAC 
CTGTCTGCAA TCACAGGAAC CAAGGATCTG AGAGTCTCTC AGGTGGTCCA CAAGGCTGTG 
CTGGACGTGG CTGAGACAGG CACAGAAGCA GCTGCTGCCA CTGGAGTCAA ATTTGTCCCA 
ATGTCTGCGA AACTGTACCC TCTGACTGTA TATTTCAATC GGCCTTTCCT GATAATGATC 
TTTGACACAG AAACTGAAAT TGCCCCCTTT ATAGCCAAGA TAGCCAACCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033278.2
CDS	106..1362
Translation	MAFIAALGLLMAGICPAVLCFPDGTLGMDAAVQEDHDNGTQLDSLTLASINTDFAFSLYKELVLKNPDKNIVFSPLSISAALAVMSLGAKGNTLEEILEGLKFNLTETSEADIHQGFGHLLQRLNQPKDQVQISTGSALFIEKRQQILTEFQEKARALYQAEAFTADFQQPRQAKKLINDYVRKQTQGMIKELVSDLDKRTLMVLVNYIYFKAKWKVPFDPLDTFKSEFYAGKRRPVIVPMMSMEDLTTPYFRDEELFCTVVELKYTGNASAMFILPDQGKMQQVEASLQPETLRKWKNSLKPRMIDELHLPKFSISTDYSLEDVLSKLGIREVFSTQADLSAITGTKDLRVSQVVHKAVLDVAETGTEAAAATGVKFVPMSAKLYPLTVYFNRPFLIMIFDTETEIAPFIAKIANPK

Target ORF information:

RefSeq Version	NM_009252.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009252.2

ORF Insert Sequence:

ATGGCCTTCA TTGCAGCTCT GGGGCTCTTG ATGGCTGGGA TCTGCCCTGC TGTCCTCTGC 
TTCCCAGATG GCACGTTGGG AATGGATGCT GCAGTCCAAG AAGACCATGA CAATGGGACA 
CAACTGGACA GTCTCACATT GGCCTCCATC AACACTGACT TTGCCTTCAG CCTCTACAAG 
GAGCTGGTTT TGAAGAATCC AGATAAAAAT ATTGTCTTCT CCCCACTTAG CATCTCAGCG 
GCCTTGGCCG TCATGTCCCT GGGAGCAAAG GGCAACACCC TGGAAGAGAT TCTAGAAGGT 
CTCAAGTTCA ATCTTACAGA GACCTCTGAG GCAGACATCC ACCAGGGCTT TGGGCACCTC 
CTACAGAGGC TCAACCAGCC AAAGGACCAG GTACAGATCA GCACGGGTAG TGCCCTGTTT 
ATTGAAAAGC GCCAGCAGAT CCTGACAGAA TTCCAGGAGA AGGCAAGGGC TCTGTACCAG 
GCTGAGGCCT TCACAGCAGA CTTCCAGCAG CCTCGTCAGG CCAAAAAGCT CATCAATGAC 
TATGTGAGGA AACAGACCCA GGGGATGATC AAGGAACTGG TCTCAGACCT GGATAAAAGG 
ACATTGATGG TGCTGGTGAA TTATATCTAC TTTAAAGCCA AATGGAAGGT GCCCTTTGAC 
CCTCTTGACA CGTTCAAGTC TGAGTTCTAC GCGGGCAAGA GGAGGCCCGT GATAGTGCCC 
ATGATGAGCA TGGAGGACCT GACCACACCC TACTTCCGAG ATGAGGAGCT TTTCTGCACT 
GTGGTGGAGC TGAAGTACAC AGGAAATGCC AGTGCCATGT TCATCCTCCC TGACCAGGGC 
AAGATGCAGC AGGTGGAAGC CAGCTTGCAA CCAGAGACCC TGAGGAAGTG GAAGAATTCT 
CTGAAACCCA GGATGATAGA TGAGCTCCAC CTGCCCAAGT TCTCCATCTC CACCGACTAC 
AGCCTGGAGG ATGTCCTTTC AAAGCTGGGC ATCAGGGAAG TCTTCTCCAC ACAGGCTGAC 
CTGTCTGCAA TCACAGGAAC CAAGGATCTG AGAGTCTCTC AGGTGGTCCA CAAGGCTGTG 
CTGGACGTGG CTGAGACAGG CACAGAAGCA GCTGCTGCCA CTGGAGTCAA ATTTGTCCCA 
ATGTCTGCGA AACTGTACCC TCTGACTGTA TATTTCAATC GGCCTTTCCT GATAATGATC 
TTTGACACAG AAACTGAAAT TGCCCCCTTT ATAGCCAAGA TAGCCAACCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Serpina3n ( XM_011244046.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242348.1 Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03798
Clone ID Related Accession (Same CDS sequence)	NM_009252.2 , XM_011244046.3 , XM_011244046.2
Accession Version	XM_011244046.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor A3N isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTCA TTGCAGCTCT GGGGCTCTTG ATGGCTGGGA TCTGCCCTGC TGTCCTCTGC 
TTCCCAGATG GCACGTTGGG AATGGATGCT GCAGTCCAAG AAGACCATGA CAATGGGACA 
CAACTGGACA GTCTCACATT GGCCTCCATC AACACTGACT TTGCCTTCAG CCTCTACAAG 
GAGCTGGTTT TGAAGAATCC AGATAAAAAT ATTGTCTTCT CCCCACTTAG CATCTCAGCG 
GCCTTGGCCG TCATGTCCCT GGGAGCAAAG GGCAACACCC TGGAAGAGAT TCTAGAAGGT 
CTCAAGTTCA ATCTTACAGA GACCTCTGAG GCAGACATCC ACCAGGGCTT TGGGCACCTC 
CTACAGAGGC TCAACCAGCC AAAGGACCAG GTACAGATCA GCACGGGTAG TGCCCTGTTT 
ATTGAAAAGC GCCAGCAGAT CCTGACAGAA TTCCAGGAGA AGGCAAGGGC TCTGTACCAG 
GCTGAGGCCT TCACAGCAGA CTTCCAGCAG CCTCGTCAGG CCAAAAAGCT CATCAATGAC 
TATGTGAGGA AACAGACCCA GGGGATGATC AAGGAACTGG TCTCAGACCT GGATAAAAGG 
ACATTGATGG TGCTGGTGAA TTATATCTAC TTTAAAGCCA AATGGAAGGT GCCCTTTGAC 
CCTCTTGACA CGTTCAAGTC TGAGTTCTAC GCGGGCAAGA GGAGGCCCGT GATAGTGCCC 
ATGATGAGCA TGGAGGACCT GACCACACCC TACTTCCGAG ATGAGGAGCT TTTCTGCACT 
GTGGTGGAGC TGAAGTACAC AGGAAATGCC AGTGCCATGT TCATCCTCCC TGACCAGGGC 
AAGATGCAGC AGGTGGAAGC CAGCTTGCAA CCAGAGACCC TGAGGAAGTG GAAGAATTCT 
CTGAAACCCA GGATGATAGA TGAGCTCCAC CTGCCCAAGT TCTCCATCTC CACCGACTAC 
AGCCTGGAGG ATGTCCTTTC AAAGCTGGGC ATCAGGGAAG TCTTCTCCAC ACAGGCTGAC 
CTGTCTGCAA TCACAGGAAC CAAGGATCTG AGAGTCTCTC AGGTGGTCCA CAAGGCTGTG 
CTGGACGTGG CTGAGACAGG CACAGAAGCA GCTGCTGCCA CTGGAGTCAA ATTTGTCCCA 
ATGTCTGCGA AACTGTACCC TCTGACTGTA TATTTCAATC GGCCTTTCCT GATAATGATC 
TTTGACACAG AAACTGAAAT TGCCCCCTTT ATAGCCAAGA TAGCCAACCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242348.1
CDS	112..1368
Translation	MAFIAALGLLMAGICPAVLCFPDGTLGMDAAVQEDHDNGTQLDSLTLASINTDFAFSLYKELVLKNPDKNIVFSPLSISAALAVMSLGAKGNTLEEILEGLKFNLTETSEADIHQGFGHLLQRLNQPKDQVQISTGSALFIEKRQQILTEFQEKARALYQAEAFTADFQQPRQAKKLINDYVRKQTQGMIKELVSDLDKRTLMVLVNYIYFKAKWKVPFDPLDTFKSEFYAGKRRPVIVPMMSMEDLTTPYFRDEELFCTVVELKYTGNASAMFILPDQGKMQQVEASLQPETLRKWKNSLKPRMIDELHLPKFSISTDYSLEDVLSKLGIREVFSTQADLSAITGTKDLRVSQVVHKAVLDVAETGTEAAAATGVKFVPMSAKLYPLTVYFNRPFLIMIFDTETEIAPFIAKIANPK

Target ORF information:

RefSeq Version	XM_011244046.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244046.2

ORF Insert Sequence:

ATGGCCTTCA TTGCAGCTCT GGGGCTCTTG ATGGCTGGGA TCTGCCCTGC TGTCCTCTGC 
TTCCCAGATG GCACGTTGGG AATGGATGCT GCAGTCCAAG AAGACCATGA CAATGGGACA 
CAACTGGACA GTCTCACATT GGCCTCCATC AACACTGACT TTGCCTTCAG CCTCTACAAG 
GAGCTGGTTT TGAAGAATCC AGATAAAAAT ATTGTCTTCT CCCCACTTAG CATCTCAGCG 
GCCTTGGCCG TCATGTCCCT GGGAGCAAAG GGCAACACCC TGGAAGAGAT TCTAGAAGGT 
CTCAAGTTCA ATCTTACAGA GACCTCTGAG GCAGACATCC ACCAGGGCTT TGGGCACCTC 
CTACAGAGGC TCAACCAGCC AAAGGACCAG GTACAGATCA GCACGGGTAG TGCCCTGTTT 
ATTGAAAAGC GCCAGCAGAT CCTGACAGAA TTCCAGGAGA AGGCAAGGGC TCTGTACCAG 
GCTGAGGCCT TCACAGCAGA CTTCCAGCAG CCTCGTCAGG CCAAAAAGCT CATCAATGAC 
TATGTGAGGA AACAGACCCA GGGGATGATC AAGGAACTGG TCTCAGACCT GGATAAAAGG 
ACATTGATGG TGCTGGTGAA TTATATCTAC TTTAAAGCCA AATGGAAGGT GCCCTTTGAC 
CCTCTTGACA CGTTCAAGTC TGAGTTCTAC GCGGGCAAGA GGAGGCCCGT GATAGTGCCC 
ATGATGAGCA TGGAGGACCT GACCACACCC TACTTCCGAG ATGAGGAGCT TTTCTGCACT 
GTGGTGGAGC TGAAGTACAC AGGAAATGCC AGTGCCATGT TCATCCTCCC TGACCAGGGC 
AAGATGCAGC AGGTGGAAGC CAGCTTGCAA CCAGAGACCC TGAGGAAGTG GAAGAATTCT 
CTGAAACCCA GGATGATAGA TGAGCTCCAC CTGCCCAAGT TCTCCATCTC CACCGACTAC 
AGCCTGGAGG ATGTCCTTTC AAAGCTGGGC ATCAGGGAAG TCTTCTCCAC ACAGGCTGAC 
CTGTCTGCAA TCACAGGAAC CAAGGATCTG AGAGTCTCTC AGGTGGTCCA CAAGGCTGTG 
CTGGACGTGG CTGAGACAGG CACAGAAGCA GCTGCTGCCA CTGGAGTCAA ATTTGTCCCA 
ATGTCTGCGA AACTGTACCC TCTGACTGTA TATTTCAATC GGCCTTTCCT GATAATGATC 
TTTGACACAG AAACTGAAAT TGCCCCCTTT ATAGCCAAGA TAGCCAACCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Serpina3n ( XM_011244046.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242348.1 Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03798
Clone ID Related Accession (Same CDS sequence)	NM_009252.2 , XM_011244046.3 , XM_011244046.2
Accession Version	XM_011244046.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor A3N isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTCA TTGCAGCTCT GGGGCTCTTG ATGGCTGGGA TCTGCCCTGC TGTCCTCTGC 
TTCCCAGATG GCACGTTGGG AATGGATGCT GCAGTCCAAG AAGACCATGA CAATGGGACA 
CAACTGGACA GTCTCACATT GGCCTCCATC AACACTGACT TTGCCTTCAG CCTCTACAAG 
GAGCTGGTTT TGAAGAATCC AGATAAAAAT ATTGTCTTCT CCCCACTTAG CATCTCAGCG 
GCCTTGGCCG TCATGTCCCT GGGAGCAAAG GGCAACACCC TGGAAGAGAT TCTAGAAGGT 
CTCAAGTTCA ATCTTACAGA GACCTCTGAG GCAGACATCC ACCAGGGCTT TGGGCACCTC 
CTACAGAGGC TCAACCAGCC AAAGGACCAG GTACAGATCA GCACGGGTAG TGCCCTGTTT 
ATTGAAAAGC GCCAGCAGAT CCTGACAGAA TTCCAGGAGA AGGCAAGGGC TCTGTACCAG 
GCTGAGGCCT TCACAGCAGA CTTCCAGCAG CCTCGTCAGG CCAAAAAGCT CATCAATGAC 
TATGTGAGGA AACAGACCCA GGGGATGATC AAGGAACTGG TCTCAGACCT GGATAAAAGG 
ACATTGATGG TGCTGGTGAA TTATATCTAC TTTAAAGCCA AATGGAAGGT GCCCTTTGAC 
CCTCTTGACA CGTTCAAGTC TGAGTTCTAC GCGGGCAAGA GGAGGCCCGT GATAGTGCCC 
ATGATGAGCA TGGAGGACCT GACCACACCC TACTTCCGAG ATGAGGAGCT TTTCTGCACT 
GTGGTGGAGC TGAAGTACAC AGGAAATGCC AGTGCCATGT TCATCCTCCC TGACCAGGGC 
AAGATGCAGC AGGTGGAAGC CAGCTTGCAA CCAGAGACCC TGAGGAAGTG GAAGAATTCT 
CTGAAACCCA GGATGATAGA TGAGCTCCAC CTGCCCAAGT TCTCCATCTC CACCGACTAC 
AGCCTGGAGG ATGTCCTTTC AAAGCTGGGC ATCAGGGAAG TCTTCTCCAC ACAGGCTGAC 
CTGTCTGCAA TCACAGGAAC CAAGGATCTG AGAGTCTCTC AGGTGGTCCA CAAGGCTGTG 
CTGGACGTGG CTGAGACAGG CACAGAAGCA GCTGCTGCCA CTGGAGTCAA ATTTGTCCCA 
ATGTCTGCGA AACTGTACCC TCTGACTGTA TATTTCAATC GGCCTTTCCT GATAATGATC 
TTTGACACAG AAACTGAAAT TGCCCCCTTT ATAGCCAAGA TAGCCAACCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242348.1
CDS	164..1420
Translation	MAFIAALGLLMAGICPAVLCFPDGTLGMDAAVQEDHDNGTQLDSLTLASINTDFAFSLYKELVLKNPDKNIVFSPLSISAALAVMSLGAKGNTLEEILEGLKFNLTETSEADIHQGFGHLLQRLNQPKDQVQISTGSALFIEKRQQILTEFQEKARALYQAEAFTADFQQPRQAKKLINDYVRKQTQGMIKELVSDLDKRTLMVLVNYIYFKAKWKVPFDPLDTFKSEFYAGKRRPVIVPMMSMEDLTTPYFRDEELFCTVVELKYTGNASAMFILPDQGKMQQVEASLQPETLRKWKNSLKPRMIDELHLPKFSISTDYSLEDVLSKLGIREVFSTQADLSAITGTKDLRVSQVVHKAVLDVAETGTEAAAATGVKFVPMSAKLYPLTVYFNRPFLIMIFDTETEIAPFIAKIANPK

Target ORF information:

RefSeq Version	XM_011244046.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244046.3

ORF Insert Sequence:

ATGGCCTTCA TTGCAGCTCT GGGGCTCTTG ATGGCTGGGA TCTGCCCTGC TGTCCTCTGC 
TTCCCAGATG GCACGTTGGG AATGGATGCT GCAGTCCAAG AAGACCATGA CAATGGGACA 
CAACTGGACA GTCTCACATT GGCCTCCATC AACACTGACT TTGCCTTCAG CCTCTACAAG 
GAGCTGGTTT TGAAGAATCC AGATAAAAAT ATTGTCTTCT CCCCACTTAG CATCTCAGCG 
GCCTTGGCCG TCATGTCCCT GGGAGCAAAG GGCAACACCC TGGAAGAGAT TCTAGAAGGT 
CTCAAGTTCA ATCTTACAGA GACCTCTGAG GCAGACATCC ACCAGGGCTT TGGGCACCTC 
CTACAGAGGC TCAACCAGCC AAAGGACCAG GTACAGATCA GCACGGGTAG TGCCCTGTTT 
ATTGAAAAGC GCCAGCAGAT CCTGACAGAA TTCCAGGAGA AGGCAAGGGC TCTGTACCAG 
GCTGAGGCCT TCACAGCAGA CTTCCAGCAG CCTCGTCAGG CCAAAAAGCT CATCAATGAC 
TATGTGAGGA AACAGACCCA GGGGATGATC AAGGAACTGG TCTCAGACCT GGATAAAAGG 
ACATTGATGG TGCTGGTGAA TTATATCTAC TTTAAAGCCA AATGGAAGGT GCCCTTTGAC 
CCTCTTGACA CGTTCAAGTC TGAGTTCTAC GCGGGCAAGA GGAGGCCCGT GATAGTGCCC 
ATGATGAGCA TGGAGGACCT GACCACACCC TACTTCCGAG ATGAGGAGCT TTTCTGCACT 
GTGGTGGAGC TGAAGTACAC AGGAAATGCC AGTGCCATGT TCATCCTCCC TGACCAGGGC 
AAGATGCAGC AGGTGGAAGC CAGCTTGCAA CCAGAGACCC TGAGGAAGTG GAAGAATTCT 
CTGAAACCCA GGATGATAGA TGAGCTCCAC CTGCCCAAGT TCTCCATCTC CACCGACTAC 
AGCCTGGAGG ATGTCCTTTC AAAGCTGGGC ATCAGGGAAG TCTTCTCCAC ACAGGCTGAC 
CTGTCTGCAA TCACAGGAAC CAAGGATCTG AGAGTCTCTC AGGTGGTCCA CAAGGCTGTG 
CTGGACGTGG CTGAGACAGG CACAGAAGCA GCTGCTGCCA CTGGAGTCAA ATTTGTCCCA 
ATGTCTGCGA AACTGTACCC TCTGACTGTA TATTTCAATC GGCCTTTCCT GATAATGATC 
TTTGACACAG AAACTGAAAT TGCCCCCTTT ATAGCCAAGA TAGCCAACCC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Trim33 Binds and Silences a Class of Young Endogenous Retroviruses in the Mouse Testis; a Novel Component of the Arms Race between Retrotransposons and the Host Genome.
PLoS genetics11(12)e1005693(2015 Dec)
Isbel L,Srivastava R,Oey H,Spurling A,Daxinger L,Puthalakath H,Whitelaw E

The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase.
Nature medicine21(5)518-23(2015 May)
Vicu?a L,Strochlic DE,Latremoliere A,Bali KK,Simonetti M,Husainie D,Prokosch S,Riva P,Griffin RS,Njoo C,Gehrig S,Mall MA,Arnold B,Devor M,Woolf CJ,Liberles SD,Costigan M,Kuner R

Serine protease inhibitors serpina1 and serpina3 are down-regulated in bone marrow during hematopoietic progenitor mobilization.
The Journal of experimental medicine201(7)1077-88(2005 Apr)
Winkler IG,Hendy J,Coughlin P,Horvath A,L?vesque JP

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Serpina3n cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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