Sp4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sp4
Entrez Gene ID	20688
Full Name	trans-acting transcription factor 4
Synonyms	5730497N03Rik,HF-1b,HF1-b
General protein information	Preferred Names trans-acting transcription factor 4 Names transcription factor Sp4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 F2\|12 63.48 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017315007.2	XP_017170496.1	transcription factor Sp4 isoform X1
NM_009239.4	NP_033265.4	transcription factor Sp4 isoform 1
NM_001166385.2	NP_001159857.2	transcription factor Sp4 isoform 2
XM_017315007.1	XP_017170496.1	transcription factor Sp4 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SP4	XP_002707857.3
Mus musculus (house mouse)	Sp4	NP_033265.3
Gallus gallus (chicken)	SP4	XP_418708.2
Xenopus tropicalis (tropical clawed frog)	sp4	XP_002933342.2
Rattus norvegicus (Norway rat)	Sp4	NP_036893.1
Canis lupus familiaris (dog)	SP4	XP_539462.1
Danio rerio (zebrafish)	sp4	NP_956418.1
Homo sapiens (human)	SP4	NP_003103.2
Pan troglodytes (chimpanzee)	SP4	XP_527679.1
	SP4	XP_001101092.2

Gene Ontology
Bibliography

Related articles in PubMed

Sp2 is the only glutamine-rich specificity protein with minor impact on development and differentiation in myelinating glia.
Wegener A, K?spert M, Sock E, Philipsen S, Suske G, Wegner M
Journal of neurochemistry140(2)245-256(2017 Jan)

Restoration of Sp4 in Forebrain GABAergic Neurons Rescues Hypersensitivity to Ketamine in Sp4 Hypomorphic Mice.
Higa KK, Ji B, Buell MR, Risbrough VB, Powell SB, Young JW, Geyer MA, Zhou X
The international journal of neuropsychopharmacology18(11)pyv063(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The transcription factor Specificity Protein 4 (SP4) is important for neurodevelopment and is genetically associated with both schizophrenia and bipolar disorder.
Title: GlyT-1 Inhibition Attenuates Attentional But Not Learning or Motivational Deficits of the Sp4 Hypomorphic Mouse Model Relevant to Psychiatric Disorders.

Results suggest that the Sp4 gene in forebrain GABAergic neurons regulates ketamine-induced hyperlocomotion
Title: Restoration of Sp4 in Forebrain GABAergic Neurons Rescues Hypersensitivity to Ketamine in Sp4 Hypomorphic Mice.

Sp4 functionally regulated GluN1, GluN2A, and GluN2B, but not GluN2C.
Title: Specificity protein 4 functionally regulates the transcription of NMDA receptor subunits GluN1, GluN2A, and GluN2B.

neuron-specific Sp4 regulates the expression of all 13 subunits of mitochondrial cytochrome c oxidase(COX) enzyme in primary neurons. Sp4 also regulates mitochondrial transcription factors (TFAM, TFB1M, and TFB2M) and a COX assembly protein SURF-1.
Title: Neuron-specific specificity protein 4 bigenomically regulates the transcription of all mitochondria- and nucleus-encoded cytochrome c oxidase subunit genes in neurons.

elucidation of the Sp4 molecular pathway in Sp4 hypomorphic mice may provide novel insights to our understanding of abnormal NMDAR signaling in schizophrenia
Title: Prolonged Ketamine Effects in Sp4 Hypomorphic Mice: Mimicking Phenotypes of Schizophrenia.

The following Sp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14117	NM_001166385.2 Latest version!	Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu14142	NM_009239.4 Latest version!	Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu78178	XM_017315007.2 Latest version!	Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu78178	XM_017315007.1	Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sp4 ( NM_001166385.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001159857.2 Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14117
Clone ID Related Accession (Same CDS sequence)	NM_001166385.2
Accession Version	NM_001166385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	transcription factor Sp4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY070406.1, AC163034.5, BC076630.1 and AC163032.5. On Dec 17, 2013 this sequence version replaced NM_001166385.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This difference results in a shorter isoform (2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC076630.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGATC AGAAGAAGGA GGAGGAGGAG GAGGCGGCAG CGGCGATGGC TACAGAAGGA 
GGGAAAACCT CTGAGCCAGA GAATAACAAT AAAAAACCCA AAACCTCAGG CTCCCAGGAC 
TCTCAGCCCT CTCCTCTGGC TTTACTGGCA GCTACTTGCA GCAAAATAGG GACTCCTGGT 
GAAAATCAAG CAACTGGACA ACAGCAGATT ATTATAGATC CAAGCCAAGG ATTGGTGCAA 
CTTCAGAATC AGCCACAGCA GCTAGAACTG GTGACGACGC AGCTTGCTGG AAACGCTTGG 
CAACTTGTTG CCTCCACTCC TCCTGCTTCA AAAGAAAATA ACGTTTCTCA GCCAGCTTCT 
AGTTCATCTA GTTCTTCCAG CAGTAATAAC GGGAGTTCCT CTCCTACAAA AACTAAATCA 
GGTAATCCTT CCACCCCTAA TCAATTTCAA GTCATACAAG TACAAAACCC AAGCGGTAGT 
GTACAGTACC AAGTGATCCC CCAACTTCAG ACGGTGGAAG GCCAGCAAAT TCAAATCAAC 
CCAACTAGTA GTTCATCTCT ACAGGATTTG CAGGGTCAAA TTCAGCTCAT TTCTGCAGGT 
AATAATCAAG CTATCCTCAC GGCTGCTAAC AGGACAGCTT CTGGGAATAT CCTTGCTCAA 
AACCTGGCAA ATCAGACAGT TCCAGTACAA ATTAGACCTG GCGTTTCAAT ACCATTGCAA 
TTGCAGACCC TTCCTGGTAC TCAGGCTCAA GTTGTAACAA CCCTACCAAT TAACATTGGA 
GGAGTGACTT TAGCTTTGCC AGTAATAAAT AACGTGACTG CTGGAGGAGG GACTGGACAA 
GTCGGCCAGC CTACTACTAC TACTGACAGT GGGACTTCCA ATGGGAATCA GTTAGTTTCT 
ACGCCCACCA CCAGCACTGC TCCCGCCAGT ACTATGCCTG AATCGCCCTC CTCCTCTACT 
ACCTGCACAA CCACCGCATC AACAACTCTG ACGAGCAGTG ACACATTAGT GAGCTCAGCA 
GATACAGGCC AGTATGCAAG CACATCAGCC AGTAGTTCTG AACGCACCAT TGAAGAACCA 
CAGACACCTG CTGCTACTGA GTCTGAAGCC CAGAGCTCCA GCCAGCTTCA GTCCAACGGA 
ATTCAGAATG CACAAGACCA GTCAAATTCC CTTCAACAAG TACAGATTGT AGGCCAGCCT 
ATCCTGCAGC AAATCCAGAT CCAGCAGCCT CAGCAGCAGA TTATTCAAGC TATCCCACCA 
CAGTCATTTC AGCTCCAGTC AGGGCAGACA ATTCAGACCA TCCAGCAGCA GCCTTTGCAG 
AATGTTCAAC TTCAAGCAGT AAACCCGACT CAGGTACTTA TCAGGGCTCC AACTTTAACA 
CCTTCAGGGC AAATCAGCTG GCAAACTGTC CAGGTCCAGA ATATTCAAAG TCTTTCAAAT 
TTGCAAGTTC AGAATGCTGG GTTATCCCAG CAGTTAACCA TCACCCCAGT GTCTTCAAGT 
GGTGGCACAA CTCTTGCTCA GATTGCTCCT GTGGCTGTTG CTGGTGCCCC AATAACTTTG 
AATACTGCCC AGCTTGCATC AGTGCCTAAC CTTCAGACAG TGAGCGTTGC CAACCTGGGT 
GCTGCGGGTG TTCAAGTTCA GGGAGTTCCA GTAACAATCA CTAGTGTTGC AGGTCAACAG 
CAAGGACAGG ATGGAGTGAA AGTCCAACAA GCTACTATAG CTCCTGTAAC GGTGGCAGTT 
GGAGGGATTG CTAATGCGAC AATAGGTGCT GTTAGTCCTG ACCAACTCAC ACAAGTGCAT 
TTGCAGCAAG GCCAGCAGAC CTCTGATGCA GAGGTGCAAC CTGGCAAGAG GCTCCGAAGA 
GTTGCCTGCT CCTGTCCTAA CTGTAGGGAA GGAGAAGGAA GCAGTGAACC AGGAAAGAAG 
AAGCAGCATG TTTGCCACAT TGAAGGGTGT GGGAAAGTAT ATGGCAAGAC CTCTCACCTC 
CGAGCACATC TCCGATGGCA CACTGGAGAA AGACCGTTCA TATGCAACTG GATGTTTTGT 
GGCAAAAGAT TCACAAGGAG CGATGAGCTT CAGAGACACA GAAGGACCCA CACAGGTGAA 
AAGAGATTTG AATGTCCAGA GTGTTCTAAA AGGTTTATGC GGAGTGATCA CCTATCCAAG 
CATGTCAAAA CTCACCAGAA TAAGAAAGGA GGTGGGACAG CTCTTGCCAT TGTTACCTCC 
GGAGAACTGG ACTCATCTGT TACAGAGGTG CTTGGCTCTC CACGGATTGT CACGGTTGCC 
GCCATTTCCC AAGATTCGAA TCCAGCAACT CCCAATGTTT CAACAAACAT GGAAGAATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159857.2
CDS	208..2550
Translation	MSDQKKEEEEEAAAAMATEGGKTSEPENNNKKPKTSGSQDSQPSPLALLAATCSKIGTPGENQATGQQQIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQPASSSSSSSSSNNGSSSPTKTKSGNPSTPNQFQVIQVQNPSGSVQYQVIPQLQTVEGQQIQINPTSSSSLQDLQGQIQLISAGNNQAILTAANRTASGNILAQNLANQTVPVQIRPGVSIPLQLQTLPGTQAQVVTTLPINIGGVTLALPVINNVTAGGGTGQVGQPTTTTDSGTSNGNQLVSTPTTSTAPASTMPESPSSSTTCTTTASTTLTSSDTLVSSADTGQYASTSASSSERTIEEPQTPAATESEAQSSSQLQSNGIQNAQDQSNSLQQVQIVGQPILQQIQIQQPQQQIIQAIPPQSFQLQSGQTIQTIQQQPLQNVQLQAVNPTQVLIRAPTLTPSGQISWQTVQVQNIQSLSNLQVQNAGLSQQLTITPVSSSGGTTLAQIAPVAVAGAPITLNTAQLASVPNLQTVSVANLGAAGVQVQGVPVTITSVAGQQQGQDGVKVQQATIAPVTVAVGGIANATIGAVSPDQLTQVHLQQGQQTSDAEVQPGKRLRRVACSCPNCREGEGSSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSVTEVLGSPRIVTVAAISQDSNPATPNVSTNMEEF

Target ORF information:

RefSeq Version	NM_001166385.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166385.2

ORF Insert Sequence:

ATGAGCGATC AGAAGAAGGA GGAGGAGGAG GAGGCGGCAG CGGCGATGGC TACAGAAGGA 
GGGAAAACCT CTGAGCCAGA GAATAACAAT AAAAAACCCA AAACCTCAGG CTCCCAGGAC 
TCTCAGCCCT CTCCTCTGGC TTTACTGGCA GCTACTTGCA GCAAAATAGG GACTCCTGGT 
GAAAATCAAG CAACTGGACA ACAGCAGATT ATTATAGATC CAAGCCAAGG ATTGGTGCAA 
CTTCAGAATC AGCCACAGCA GCTAGAACTG GTGACGACGC AGCTTGCTGG AAACGCTTGG 
CAACTTGTTG CCTCCACTCC TCCTGCTTCA AAAGAAAATA ACGTTTCTCA GCCAGCTTCT 
AGTTCATCTA GTTCTTCCAG CAGTAATAAC GGGAGTTCCT CTCCTACAAA AACTAAATCA 
GGTAATCCTT CCACCCCTAA TCAATTTCAA GTCATACAAG TACAAAACCC AAGCGGTAGT 
GTACAGTACC AAGTGATCCC CCAACTTCAG ACGGTGGAAG GCCAGCAAAT TCAAATCAAC 
CCAACTAGTA GTTCATCTCT ACAGGATTTG CAGGGTCAAA TTCAGCTCAT TTCTGCAGGT 
AATAATCAAG CTATCCTCAC GGCTGCTAAC AGGACAGCTT CTGGGAATAT CCTTGCTCAA 
AACCTGGCAA ATCAGACAGT TCCAGTACAA ATTAGACCTG GCGTTTCAAT ACCATTGCAA 
TTGCAGACCC TTCCTGGTAC TCAGGCTCAA GTTGTAACAA CCCTACCAAT TAACATTGGA 
GGAGTGACTT TAGCTTTGCC AGTAATAAAT AACGTGACTG CTGGAGGAGG GACTGGACAA 
GTCGGCCAGC CTACTACTAC TACTGACAGT GGGACTTCCA ATGGGAATCA GTTAGTTTCT 
ACGCCCACCA CCAGCACTGC TCCCGCCAGT ACTATGCCTG AATCGCCCTC CTCCTCTACT 
ACCTGCACAA CCACCGCATC AACAACTCTG ACGAGCAGTG ACACATTAGT GAGCTCAGCA 
GATACAGGCC AGTATGCAAG CACATCAGCC AGTAGTTCTG AACGCACCAT TGAAGAACCA 
CAGACACCTG CTGCTACTGA GTCTGAAGCC CAGAGCTCCA GCCAGCTTCA GTCCAACGGA 
ATTCAGAATG CACAAGACCA GTCAAATTCC CTTCAACAAG TACAGATTGT AGGCCAGCCT 
ATCCTGCAGC AAATCCAGAT CCAGCAGCCT CAGCAGCAGA TTATTCAAGC TATCCCACCA 
CAGTCATTTC AGCTCCAGTC AGGGCAGACA ATTCAGACCA TCCAGCAGCA GCCTTTGCAG 
AATGTTCAAC TTCAAGCAGT AAACCCGACT CAGGTACTTA TCAGGGCTCC AACTTTAACA 
CCTTCAGGGC AAATCAGCTG GCAAACTGTC CAGGTCCAGA ATATTCAAAG TCTTTCAAAT 
TTGCAAGTTC AGAATGCTGG GTTATCCCAG CAGTTAACCA TCACCCCAGT GTCTTCAAGT 
GGTGGCACAA CTCTTGCTCA GATTGCTCCT GTGGCTGTTG CTGGTGCCCC AATAACTTTG 
AATACTGCCC AGCTTGCATC AGTGCCTAAC CTTCAGACAG TGAGCGTTGC CAACCTGGGT 
GCTGCGGGTG TTCAAGTTCA GGGAGTTCCA GTAACAATCA CTAGTGTTGC AGGTCAACAG 
CAAGGACAGG ATGGAGTGAA AGTCCAACAA GCTACTATAG CTCCTGTAAC GGTGGCAGTT 
GGAGGGATTG CTAATGCGAC AATAGGTGCT GTTAGTCCTG ACCAACTCAC ACAAGTGCAT 
TTGCAGCAAG GCCAGCAGAC CTCTGATGCA GAGGTGCAAC CTGGCAAGAG GCTCCGAAGA 
GTTGCCTGCT CCTGTCCTAA CTGTAGGGAA GGAGAAGGAA GCAGTGAACC AGGAAAGAAG 
AAGCAGCATG TTTGCCACAT TGAAGGGTGT GGGAAAGTAT ATGGCAAGAC CTCTCACCTC 
CGAGCACATC TCCGATGGCA CACTGGAGAA AGACCGTTCA TATGCAACTG GATGTTTTGT 
GGCAAAAGAT TCACAAGGAG CGATGAGCTT CAGAGACACA GAAGGACCCA CACAGGTGAA 
AAGAGATTTG AATGTCCAGA GTGTTCTAAA AGGTTTATGC GGAGTGATCA CCTATCCAAG 
CATGTCAAAA CTCACCAGAA TAAGAAAGGA GGTGGGACAG CTCTTGCCAT TGTTACCTCC 
GGAGAACTGG ACTCATCTGT TACAGAGGTG CTTGGCTCTC CACGGATTGT CACGGTTGCC 
GCCATTTCCC AAGATTCGAA TCCAGCAACT CCCAATGTTT CAACAAACAT GGAAGAATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sp4 ( NM_009239.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033265.4 Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14142
Clone ID Related Accession (Same CDS sequence)	NM_009239.4
Accession Version	NM_009239.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	transcription factor Sp4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY070406.1, AC163034.5, BC076630.1 and AC163032.5. On Dec 17, 2013 this sequence version replaced NM_009239.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U62522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGATC AGAAGAAGGA GGAGGAGGAG GAGGCGGCAG CGGCGATGGC TACAGAAGGA 
GGGAAAACCT CTGAGCCAGA GAATAACAAT AAAAAACCCA AAACCTCAGG CTCCCAGGAC 
TCTCAGCCCT CTCCTCTGGC TTTACTGGCA GCTACTTGCA GCAAAATAGG GACTCCTGGT 
GAAAATCAAG CAACTGGACA ACAGCAGATT ATTATAGATC CAAGCCAAGG ATTGGTGCAA 
CTTCAGAATC AGCCACAGCA GCTAGAACTG GTGACGACGC AGCTTGCTGG AAACGCTTGG 
CAACTTGTTG CCTCCACTCC TCCTGCTTCA AAAGAAAATA ACGTTTCTCA GCCAGCTTCT 
AGTTCATCTA GTTCTTCCAG CAGTAATAAC GGGAGTTCCT CTCCTACAAA AACTAAATCA 
GGTAATCCTT CCACCCCTAA TCAATTTCAA GTCATACAAG TACAAAACCC AAGCGGTAGT 
GTACAGTACC AAGTGATCCC CCAACTTCAG ACGGTGGAAG GCCAGCAAAT TCAAATCAAC 
CCAACTAGTA GTTCATCTCT ACAGGATTTG CAGGGTCAAA TTCAGCTCAT TTCTGCAGGT 
AATAATCAAG CTATCCTCAC GGCTGCTAAC AGGACAGCTT CTGGGAATAT CCTTGCTCAA 
AACCTGGCAA ATCAGACAGT TCCAGTACAA ATTAGACCTG GCGTTTCAAT ACCATTGCAA 
TTGCAGACCC TTCCTGGTAC TCAGGCTCAA GTTGTAACAA CCCTACCAAT TAACATTGGA 
GGAGTGACTT TAGCTTTGCC AGTAATAAAT AACGTGACTG CTGGAGGAGG GACTGGACAA 
GTCGGCCAGC CTACTACTAC TACTGACAGT GGGACTTCCA ATGGGAATCA GTTAGTTTCT 
ACGCCCACCA CCAGCACTGC TCCCGCCAGT ACTATGCCTG AATCGCCCTC CTCCTCTACT 
ACCTGCACAA CCACCGCATC AACAACTCTG ACGAGCAGTG ACACATTAGT GAGCTCAGCA 
GATACAGGCC AGTATGCAAG CACATCAGCC AGTAGTTCTG AACGCACCAT TGAAGAACCA 
CAGACACCTG CTGCTACTGA GTCTGAAGCC CAGAGCTCCA GCCAGCTTCA GTCCAACGGA 
ATTCAGAATG CACAAGACCA GTCAAATTCC CTTCAACAAG TACAGATTGT AGGCCAGCCT 
ATCCTGCAGC AAATCCAGAT CCAGCAGCCT CAGCAGCAGA TTATTCAAGC TATCCCACCA 
CAGTCATTTC AGCTCCAGTC AGGGCAGACA ATTCAGACCA TCCAGCAGCA GCCTTTGCAG 
AATGTTCAAC TTCAAGCAGT AAACCCGACT CAGGTACTTA TCAGGGCTCC AACTTTAACA 
CCTTCAGGGC AAATCAGCTG GCAAACTGTC CAGGTCCAGA ATATTCAAAG TCTTTCAAAT 
TTGCAAGTTC AGAATGCTGG GTTATCCCAG CAGTTAACCA TCACCCCAGT GTCTTCAAGT 
GGTGGCACAA CTCTTGCTCA GATTGCTCCT GTGGCTGTTG CTGGTGCCCC AATAACTTTG 
AATACTGCCC AGCTTGCATC AGTGCCTAAC CTTCAGACAG TGAGCGTTGC CAACCTGGGT 
GCTGCGGGTG TTCAAGTTCA GGGAGTTCCA GTAACAATCA CTAGTGTTGC AGGTCAACAG 
CAAGGACAGG ATGGAGTGAA AGTCCAACAA GCTACTATAG CTCCTGTAAC GGTGGCAGTT 
GGAGGGATTG CTAATGCGAC AATAGGTGCT GTTAGTCCTG ACCAACTCAC ACAAGTGCAT 
TTGCAGCAAG GCCAGCAGAC CTCTGATGCA GAGGTGCAAC CTGGCAAGAG GCTCCGAAGA 
GTTGCCTGCT CCTGTCCTAA CTGTAGGGAA GGAGAAGGAA GGGGCAGCAG TGAACCAGGA 
AAGAAGAAGC AGCATGTTTG CCACATTGAA GGGTGTGGGA AAGTATATGG CAAGACCTCT 
CACCTCCGAG CACATCTCCG ATGGCACACT GGAGAAAGAC CGTTCATATG CAACTGGATG 
TTTTGTGGCA AAAGATTCAC AAGGAGCGAT GAGCTTCAGA GACACAGAAG GACCCACACA 
GGTGAAAAGA GATTTGAATG TCCAGAGTGT TCTAAAAGGT TTATGCGGAG TGATCACCTA 
TCCAAGCATG TCAAAACTCA CCAGAATAAG AAAGGAGGTG GGACAGCTCT TGCCATTGTT 
ACCTCCGGAG AACTGGACTC ATCTGTTACA GAGGTGCTTG GCTCTCCACG GATTGTCACG 
GTTGCCGCCA TTTCCCAAGA TTCGAATCCA GCAACTCCCA ATGTTTCAAC AAACATGGAA 
GAATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033265.4
CDS	208..2556
Translation	MSDQKKEEEEEAAAAMATEGGKTSEPENNNKKPKTSGSQDSQPSPLALLAATCSKIGTPGENQATGQQQIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQPASSSSSSSSSNNGSSSPTKTKSGNPSTPNQFQVIQVQNPSGSVQYQVIPQLQTVEGQQIQINPTSSSSLQDLQGQIQLISAGNNQAILTAANRTASGNILAQNLANQTVPVQIRPGVSIPLQLQTLPGTQAQVVTTLPINIGGVTLALPVINNVTAGGGTGQVGQPTTTTDSGTSNGNQLVSTPTTSTAPASTMPESPSSSTTCTTTASTTLTSSDTLVSSADTGQYASTSASSSERTIEEPQTPAATESEAQSSSQLQSNGIQNAQDQSNSLQQVQIVGQPILQQIQIQQPQQQIIQAIPPQSFQLQSGQTIQTIQQQPLQNVQLQAVNPTQVLIRAPTLTPSGQISWQTVQVQNIQSLSNLQVQNAGLSQQLTITPVSSSGGTTLAQIAPVAVAGAPITLNTAQLASVPNLQTVSVANLGAAGVQVQGVPVTITSVAGQQQGQDGVKVQQATIAPVTVAVGGIANATIGAVSPDQLTQVHLQQGQQTSDAEVQPGKRLRRVACSCPNCREGEGRGSSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSVTEVLGSPRIVTVAAISQDSNPATPNVSTNMEEF

Target ORF information:

RefSeq Version	NM_009239.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009239.4

ORF Insert Sequence:

ATGAGCGATC AGAAGAAGGA GGAGGAGGAG GAGGCGGCAG CGGCGATGGC TACAGAAGGA 
GGGAAAACCT CTGAGCCAGA GAATAACAAT AAAAAACCCA AAACCTCAGG CTCCCAGGAC 
TCTCAGCCCT CTCCTCTGGC TTTACTGGCA GCTACTTGCA GCAAAATAGG GACTCCTGGT 
GAAAATCAAG CAACTGGACA ACAGCAGATT ATTATAGATC CAAGCCAAGG ATTGGTGCAA 
CTTCAGAATC AGCCACAGCA GCTAGAACTG GTGACGACGC AGCTTGCTGG AAACGCTTGG 
CAACTTGTTG CCTCCACTCC TCCTGCTTCA AAAGAAAATA ACGTTTCTCA GCCAGCTTCT 
AGTTCATCTA GTTCTTCCAG CAGTAATAAC GGGAGTTCCT CTCCTACAAA AACTAAATCA 
GGTAATCCTT CCACCCCTAA TCAATTTCAA GTCATACAAG TACAAAACCC AAGCGGTAGT 
GTACAGTACC AAGTGATCCC CCAACTTCAG ACGGTGGAAG GCCAGCAAAT TCAAATCAAC 
CCAACTAGTA GTTCATCTCT ACAGGATTTG CAGGGTCAAA TTCAGCTCAT TTCTGCAGGT 
AATAATCAAG CTATCCTCAC GGCTGCTAAC AGGACAGCTT CTGGGAATAT CCTTGCTCAA 
AACCTGGCAA ATCAGACAGT TCCAGTACAA ATTAGACCTG GCGTTTCAAT ACCATTGCAA 
TTGCAGACCC TTCCTGGTAC TCAGGCTCAA GTTGTAACAA CCCTACCAAT TAACATTGGA 
GGAGTGACTT TAGCTTTGCC AGTAATAAAT AACGTGACTG CTGGAGGAGG GACTGGACAA 
GTCGGCCAGC CTACTACTAC TACTGACAGT GGGACTTCCA ATGGGAATCA GTTAGTTTCT 
ACGCCCACCA CCAGCACTGC TCCCGCCAGT ACTATGCCTG AATCGCCCTC CTCCTCTACT 
ACCTGCACAA CCACCGCATC AACAACTCTG ACGAGCAGTG ACACATTAGT GAGCTCAGCA 
GATACAGGCC AGTATGCAAG CACATCAGCC AGTAGTTCTG AACGCACCAT TGAAGAACCA 
CAGACACCTG CTGCTACTGA GTCTGAAGCC CAGAGCTCCA GCCAGCTTCA GTCCAACGGA 
ATTCAGAATG CACAAGACCA GTCAAATTCC CTTCAACAAG TACAGATTGT AGGCCAGCCT 
ATCCTGCAGC AAATCCAGAT CCAGCAGCCT CAGCAGCAGA TTATTCAAGC TATCCCACCA 
CAGTCATTTC AGCTCCAGTC AGGGCAGACA ATTCAGACCA TCCAGCAGCA GCCTTTGCAG 
AATGTTCAAC TTCAAGCAGT AAACCCGACT CAGGTACTTA TCAGGGCTCC AACTTTAACA 
CCTTCAGGGC AAATCAGCTG GCAAACTGTC CAGGTCCAGA ATATTCAAAG TCTTTCAAAT 
TTGCAAGTTC AGAATGCTGG GTTATCCCAG CAGTTAACCA TCACCCCAGT GTCTTCAAGT 
GGTGGCACAA CTCTTGCTCA GATTGCTCCT GTGGCTGTTG CTGGTGCCCC AATAACTTTG 
AATACTGCCC AGCTTGCATC AGTGCCTAAC CTTCAGACAG TGAGCGTTGC CAACCTGGGT 
GCTGCGGGTG TTCAAGTTCA GGGAGTTCCA GTAACAATCA CTAGTGTTGC AGGTCAACAG 
CAAGGACAGG ATGGAGTGAA AGTCCAACAA GCTACTATAG CTCCTGTAAC GGTGGCAGTT 
GGAGGGATTG CTAATGCGAC AATAGGTGCT GTTAGTCCTG ACCAACTCAC ACAAGTGCAT 
TTGCAGCAAG GCCAGCAGAC CTCTGATGCA GAGGTGCAAC CTGGCAAGAG GCTCCGAAGA 
GTTGCCTGCT CCTGTCCTAA CTGTAGGGAA GGAGAAGGAA GGGGCAGCAG TGAACCAGGA 
AAGAAGAAGC AGCATGTTTG CCACATTGAA GGGTGTGGGA AAGTATATGG CAAGACCTCT 
CACCTCCGAG CACATCTCCG ATGGCACACT GGAGAAAGAC CGTTCATATG CAACTGGATG 
TTTTGTGGCA AAAGATTCAC AAGGAGCGAT GAGCTTCAGA GACACAGAAG GACCCACACA 
GGTGAAAAGA GATTTGAATG TCCAGAGTGT TCTAAAAGGT TTATGCGGAG TGATCACCTA 
TCCAAGCATG TCAAAACTCA CCAGAATAAG AAAGGAGGTG GGACAGCTCT TGCCATTGTT 
ACCTCCGGAG AACTGGACTC ATCTGTTACA GAGGTGCTTG GCTCTCCACG GATTGTCACG 
GTTGCCGCCA TTTCCCAAGA TTCGAATCCA GCAACTCCCA ATGTTTCAAC AAACATGGAA 
GAATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sp4 ( XM_017315007.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170496.1 Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78178
Clone ID Related Accession (Same CDS sequence)	XM_017315007.2 , XM_017315007.1
Accession Version	XM_017315007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transcription factor Sp4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACAG AAGGAGGGAA AACCTCTGAG CCAGAGAATA ACAATAAAAA ACCCAAAACC 
TCAGGCTCCC AGGACTCTCA GCCCTCTCCT CTGGCTTTAC TGGCAGCTAC TTGCAGCAAA 
ATAGGGACTC CTGGTGAAAA TCAAGCAACT GGACAACAGC AGATTATTAT AGATCCAAGC 
CAAGGATTGG TGCAACTTCA GAATCAGCCA CAGCAGCTAG AACTGGTGAC GACGCAGCTT 
GCTGGAAACG CTTGGCAACT TGTTGCCTCC ACTCCTCCTG CTTCAAAAGA AAATAACGTT 
TCTCAGCCAG CTTCTAGTTC ATCTAGTTCT TCCAGCAGTA ATAACGGGAG TTCCTCTCCT 
ACAAAAACTA AATCAGGTAA TCCTTCCACC CCTAATCAAT TTCAAGTCAT ACAAGTACAA 
AACCCAAGCG GTAGTGTACA GTACCAAGTG ATCCCCCAAC TTCAGACGGT GGAAGGCCAG 
CAAATTCAAA TCAACCCAAC TAGTAGTTCA TCTCTACAGG ATTTGCAGGG TCAAATTCAG 
CTCATTTCTG CAGGTAATAA TCAAGCTATC CTCACGGCTG CTAACAGGAC AGCTTCTGGG 
AATATCCTTG CTCAAAACCT GGCAAATCAG ACAGTTCCAG TACAAATTAG ACCTGGCGTT 
TCAATACCAT TGCAATTGCA GACCCTTCCT GGTACTCAGG CTCAAGTTGT AACAACCCTA 
CCAATTAACA TTGGAGGAGT GACTTTAGCT TTGCCAGTAA TAAATAACGT GACTGCTGGA 
GGAGGGACTG GACAAGTCGG CCAGCCTACT ACTACTACTG ACAGTGGGAC TTCCAATGGG 
AATCAGTTAG TTTCTACGCC CACCACCAGC ACTGCTCCCG CCAGTACTAT GCCTGAATCG 
CCCTCCTCCT CTACTACCTG CACAACCACC GCATCAACAA CTCTGACGAG CAGTGACACA 
TTAGTGAGCT CAGCAGATAC AGGCCAGTAT GCAAGCACAT CAGCCAGTAG TTCTGAACGC 
ACCATTGAAG AACCACAGAC ACCTGCTGCT ACTGAGTCTG AAGCCCAGAG CTCCAGCCAG 
CTTCAGTCCA ACGGAATTCA GAATGCACAA GACCAGTCAA ATTCCCTTCA ACAAGTACAG 
ATTGTAGGCC AGCCTATCCT GCAGCAAATC CAGATCCAGC AGCCTCAGCA GCAGATTATT 
CAAGCTATCC CACCACAGTC ATTTCAGCTC CAGTCAGGGC AGACAATTCA GACCATCCAG 
CAGCAGCCTT TGCAGAATGT TCAACTTCAA GCAGTAAACC CGACTCAGGT ACTTATCAGG 
GCTCCAACTT TAACACCTTC AGGGCAAATC AGCTGGCAAA CTGTCCAGGT CCAGAATATT 
CAAAGTCTTT CAAATTTGCA AGTTCAGAAT GCTGGGTTAT CCCAGCAGTT AACCATCACC 
CCAGTGTCTT CAAGTGGTGG CACAACTCTT GCTCAGATTG CTCCTGTGGC TGTTGCTGGT 
GCCCCAATAA CTTTGAATAC TGCCCAGCTT GCATCAGTGC CTAACCTTCA GACAGTGAGC 
GTTGCCAACC TGGGTGCTGC GGGTGTTCAA GTTCAGGGAG TTCCAGTAAC AATCACTAGT 
GTTGCAGGTC AACAGCAAGG ACAGGATGGA GTGAAAGTCC AACAAGCTAC TATAGCTCCT 
GTAACGGTGG CAGTTGGAGG GATTGCTAAT GCGACAATAG GTGCTGTTAG TCCTGACCAA 
CTCACACAAG TGCATTTGCA GCAAGGCCAG CAGACCTCTG ATGCAGAGGT GCAACCTGGC 
AAGAGGCTCC GAAGAGTTGC CTGCTCCTGT CCTAACTGTA GGGAAGGAGA AGGAAGGGGC 
AGCAGTGAAC CAGGAAAGAA GAAGCAGCAT GTTTGCCACA TTGAAGGGTG TGGGAAAGTA 
TATGGCAAGA CCTCTCACCT CCGAGCACAT CTCCGATGGC ACACTGGAGA AAGACCGTTC 
ATATGCAACT GGATGTTTTG TGGCAAAAGA TTCACAAGGA GCGATGAGCT TCAGAGACAC 
AGAAGGACCC ACACAGGTGA AAAGAGATTT GAATGTCCAG AGTGTTCTAA AAGGTTTATG 
CGGAGTGATC ACCTATCCAA GCATGTCAAA ACTCACCAGA ATAAGAAAGG AGGTGGGACA 
GCTCTTGCCA TTGTTACCTC CGGAGAACTG GACTCATCTG TTACAGAGGT GCTTGGCTCT 
CCACGGATTG TCACGGTTGC CGCCATTTCC CAAGATTCGA ATCCAGCAAC TCCCAATGTT 
TCAACAAACA TGGAAGAATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170496.1
CDS	239..2542
Translation	MATEGGKTSEPENNNKKPKTSGSQDSQPSPLALLAATCSKIGTPGENQATGQQQIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQPASSSSSSSSSNNGSSSPTKTKSGNPSTPNQFQVIQVQNPSGSVQYQVIPQLQTVEGQQIQINPTSSSSLQDLQGQIQLISAGNNQAILTAANRTASGNILAQNLANQTVPVQIRPGVSIPLQLQTLPGTQAQVVTTLPINIGGVTLALPVINNVTAGGGTGQVGQPTTTTDSGTSNGNQLVSTPTTSTAPASTMPESPSSSTTCTTTASTTLTSSDTLVSSADTGQYASTSASSSERTIEEPQTPAATESEAQSSSQLQSNGIQNAQDQSNSLQQVQIVGQPILQQIQIQQPQQQIIQAIPPQSFQLQSGQTIQTIQQQPLQNVQLQAVNPTQVLIRAPTLTPSGQISWQTVQVQNIQSLSNLQVQNAGLSQQLTITPVSSSGGTTLAQIAPVAVAGAPITLNTAQLASVPNLQTVSVANLGAAGVQVQGVPVTITSVAGQQQGQDGVKVQQATIAPVTVAVGGIANATIGAVSPDQLTQVHLQQGQQTSDAEVQPGKRLRRVACSCPNCREGEGRGSSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSVTEVLGSPRIVTVAAISQDSNPATPNVSTNMEEF

Target ORF information:

RefSeq Version	XM_017315007.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315007.2

ORF Insert Sequence:

ATGGCTACAG AAGGAGGGAA AACCTCTGAG CCAGAGAATA ACAATAAAAA ACCCAAAACC 
TCAGGCTCCC AGGACTCTCA GCCCTCTCCT CTGGCTTTAC TGGCAGCTAC TTGCAGCAAA 
ATAGGGACTC CTGGTGAAAA TCAAGCAACT GGACAACAGC AGATTATTAT AGATCCAAGC 
CAAGGATTGG TGCAACTTCA GAATCAGCCA CAGCAGCTAG AACTGGTGAC GACGCAGCTT 
GCTGGAAACG CTTGGCAACT TGTTGCCTCC ACTCCTCCTG CTTCAAAAGA AAATAACGTT 
TCTCAGCCAG CTTCTAGTTC ATCTAGTTCT TCCAGCAGTA ATAACGGGAG TTCCTCTCCT 
ACAAAAACTA AATCAGGTAA TCCTTCCACC CCTAATCAAT TTCAAGTCAT ACAAGTACAA 
AACCCAAGCG GTAGTGTACA GTACCAAGTG ATCCCCCAAC TTCAGACGGT GGAAGGCCAG 
CAAATTCAAA TCAACCCAAC TAGTAGTTCA TCTCTACAGG ATTTGCAGGG TCAAATTCAG 
CTCATTTCTG CAGGTAATAA TCAAGCTATC CTCACGGCTG CTAACAGGAC AGCTTCTGGG 
AATATCCTTG CTCAAAACCT GGCAAATCAG ACAGTTCCAG TACAAATTAG ACCTGGCGTT 
TCAATACCAT TGCAATTGCA GACCCTTCCT GGTACTCAGG CTCAAGTTGT AACAACCCTA 
CCAATTAACA TTGGAGGAGT GACTTTAGCT TTGCCAGTAA TAAATAACGT GACTGCTGGA 
GGAGGGACTG GACAAGTCGG CCAGCCTACT ACTACTACTG ACAGTGGGAC TTCCAATGGG 
AATCAGTTAG TTTCTACGCC CACCACCAGC ACTGCTCCCG CCAGTACTAT GCCTGAATCG 
CCCTCCTCCT CTACTACCTG CACAACCACC GCATCAACAA CTCTGACGAG CAGTGACACA 
TTAGTGAGCT CAGCAGATAC AGGCCAGTAT GCAAGCACAT CAGCCAGTAG TTCTGAACGC 
ACCATTGAAG AACCACAGAC ACCTGCTGCT ACTGAGTCTG AAGCCCAGAG CTCCAGCCAG 
CTTCAGTCCA ACGGAATTCA GAATGCACAA GACCAGTCAA ATTCCCTTCA ACAAGTACAG 
ATTGTAGGCC AGCCTATCCT GCAGCAAATC CAGATCCAGC AGCCTCAGCA GCAGATTATT 
CAAGCTATCC CACCACAGTC ATTTCAGCTC CAGTCAGGGC AGACAATTCA GACCATCCAG 
CAGCAGCCTT TGCAGAATGT TCAACTTCAA GCAGTAAACC CGACTCAGGT ACTTATCAGG 
GCTCCAACTT TAACACCTTC AGGGCAAATC AGCTGGCAAA CTGTCCAGGT CCAGAATATT 
CAAAGTCTTT CAAATTTGCA AGTTCAGAAT GCTGGGTTAT CCCAGCAGTT AACCATCACC 
CCAGTGTCTT CAAGTGGTGG CACAACTCTT GCTCAGATTG CTCCTGTGGC TGTTGCTGGT 
GCCCCAATAA CTTTGAATAC TGCCCAGCTT GCATCAGTGC CTAACCTTCA GACAGTGAGC 
GTTGCCAACC TGGGTGCTGC GGGTGTTCAA GTTCAGGGAG TTCCAGTAAC AATCACTAGT 
GTTGCAGGTC AACAGCAAGG ACAGGATGGA GTGAAAGTCC AACAAGCTAC TATAGCTCCT 
GTAACGGTGG CAGTTGGAGG GATTGCTAAT GCGACAATAG GTGCTGTTAG TCCTGACCAA 
CTCACACAAG TGCATTTGCA GCAAGGCCAG CAGACCTCTG ATGCAGAGGT GCAACCTGGC 
AAGAGGCTCC GAAGAGTTGC CTGCTCCTGT CCTAACTGTA GGGAAGGAGA AGGAAGGGGC 
AGCAGTGAAC CAGGAAAGAA GAAGCAGCAT GTTTGCCACA TTGAAGGGTG TGGGAAAGTA 
TATGGCAAGA CCTCTCACCT CCGAGCACAT CTCCGATGGC ACACTGGAGA AAGACCGTTC 
ATATGCAACT GGATGTTTTG TGGCAAAAGA TTCACAAGGA GCGATGAGCT TCAGAGACAC 
AGAAGGACCC ACACAGGTGA AAAGAGATTT GAATGTCCAG AGTGTTCTAA AAGGTTTATG 
CGGAGTGATC ACCTATCCAA GCATGTCAAA ACTCACCAGA ATAAGAAAGG AGGTGGGACA 
GCTCTTGCCA TTGTTACCTC CGGAGAACTG GACTCATCTG TTACAGAGGT GCTTGGCTCT 
CCACGGATTG TCACGGTTGC CGCCATTTCC CAAGATTCGA ATCCAGCAAC TCCCAATGTT 
TCAACAAACA TGGAAGAATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sp4 ( XM_017315007.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170496.1 Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78178
Clone ID Related Accession (Same CDS sequence)	XM_017315007.2 , XM_017315007.1
Accession Version	XM_017315007.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transcription factor Sp4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACAG AAGGAGGGAA AACCTCTGAG CCAGAGAATA ACAATAAAAA ACCCAAAACC 
TCAGGCTCCC AGGACTCTCA GCCCTCTCCT CTGGCTTTAC TGGCAGCTAC TTGCAGCAAA 
ATAGGGACTC CTGGTGAAAA TCAAGCAACT GGACAACAGC AGATTATTAT AGATCCAAGC 
CAAGGATTGG TGCAACTTCA GAATCAGCCA CAGCAGCTAG AACTGGTGAC GACGCAGCTT 
GCTGGAAACG CTTGGCAACT TGTTGCCTCC ACTCCTCCTG CTTCAAAAGA AAATAACGTT 
TCTCAGCCAG CTTCTAGTTC ATCTAGTTCT TCCAGCAGTA ATAACGGGAG TTCCTCTCCT 
ACAAAAACTA AATCAGGTAA TCCTTCCACC CCTAATCAAT TTCAAGTCAT ACAAGTACAA 
AACCCAAGCG GTAGTGTACA GTACCAAGTG ATCCCCCAAC TTCAGACGGT GGAAGGCCAG 
CAAATTCAAA TCAACCCAAC TAGTAGTTCA TCTCTACAGG ATTTGCAGGG TCAAATTCAG 
CTCATTTCTG CAGGTAATAA TCAAGCTATC CTCACGGCTG CTAACAGGAC AGCTTCTGGG 
AATATCCTTG CTCAAAACCT GGCAAATCAG ACAGTTCCAG TACAAATTAG ACCTGGCGTT 
TCAATACCAT TGCAATTGCA GACCCTTCCT GGTACTCAGG CTCAAGTTGT AACAACCCTA 
CCAATTAACA TTGGAGGAGT GACTTTAGCT TTGCCAGTAA TAAATAACGT GACTGCTGGA 
GGAGGGACTG GACAAGTCGG CCAGCCTACT ACTACTACTG ACAGTGGGAC TTCCAATGGG 
AATCAGTTAG TTTCTACGCC CACCACCAGC ACTGCTCCCG CCAGTACTAT GCCTGAATCG 
CCCTCCTCCT CTACTACCTG CACAACCACC GCATCAACAA CTCTGACGAG CAGTGACACA 
TTAGTGAGCT CAGCAGATAC AGGCCAGTAT GCAAGCACAT CAGCCAGTAG TTCTGAACGC 
ACCATTGAAG AACCACAGAC ACCTGCTGCT ACTGAGTCTG AAGCCCAGAG CTCCAGCCAG 
CTTCAGTCCA ACGGAATTCA GAATGCACAA GACCAGTCAA ATTCCCTTCA ACAAGTACAG 
ATTGTAGGCC AGCCTATCCT GCAGCAAATC CAGATCCAGC AGCCTCAGCA GCAGATTATT 
CAAGCTATCC CACCACAGTC ATTTCAGCTC CAGTCAGGGC AGACAATTCA GACCATCCAG 
CAGCAGCCTT TGCAGAATGT TCAACTTCAA GCAGTAAACC CGACTCAGGT ACTTATCAGG 
GCTCCAACTT TAACACCTTC AGGGCAAATC AGCTGGCAAA CTGTCCAGGT CCAGAATATT 
CAAAGTCTTT CAAATTTGCA AGTTCAGAAT GCTGGGTTAT CCCAGCAGTT AACCATCACC 
CCAGTGTCTT CAAGTGGTGG CACAACTCTT GCTCAGATTG CTCCTGTGGC TGTTGCTGGT 
GCCCCAATAA CTTTGAATAC TGCCCAGCTT GCATCAGTGC CTAACCTTCA GACAGTGAGC 
GTTGCCAACC TGGGTGCTGC GGGTGTTCAA GTTCAGGGAG TTCCAGTAAC AATCACTAGT 
GTTGCAGGTC AACAGCAAGG ACAGGATGGA GTGAAAGTCC AACAAGCTAC TATAGCTCCT 
GTAACGGTGG CAGTTGGAGG GATTGCTAAT GCGACAATAG GTGCTGTTAG TCCTGACCAA 
CTCACACAAG TGCATTTGCA GCAAGGCCAG CAGACCTCTG ATGCAGAGGT GCAACCTGGC 
AAGAGGCTCC GAAGAGTTGC CTGCTCCTGT CCTAACTGTA GGGAAGGAGA AGGAAGGGGC 
AGCAGTGAAC CAGGAAAGAA GAAGCAGCAT GTTTGCCACA TTGAAGGGTG TGGGAAAGTA 
TATGGCAAGA CCTCTCACCT CCGAGCACAT CTCCGATGGC ACACTGGAGA AAGACCGTTC 
ATATGCAACT GGATGTTTTG TGGCAAAAGA TTCACAAGGA GCGATGAGCT TCAGAGACAC 
AGAAGGACCC ACACAGGTGA AAAGAGATTT GAATGTCCAG AGTGTTCTAA AAGGTTTATG 
CGGAGTGATC ACCTATCCAA GCATGTCAAA ACTCACCAGA ATAAGAAAGG AGGTGGGACA 
GCTCTTGCCA TTGTTACCTC CGGAGAACTG GACTCATCTG TTACAGAGGT GCTTGGCTCT 
CCACGGATTG TCACGGTTGC CGCCATTTCC CAAGATTCGA ATCCAGCAAC TCCCAATGTT 
TCAACAAACA TGGAAGAATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170496.1
CDS	195..2498
Translation	MATEGGKTSEPENNNKKPKTSGSQDSQPSPLALLAATCSKIGTPGENQATGQQQIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQPASSSSSSSSSNNGSSSPTKTKSGNPSTPNQFQVIQVQNPSGSVQYQVIPQLQTVEGQQIQINPTSSSSLQDLQGQIQLISAGNNQAILTAANRTASGNILAQNLANQTVPVQIRPGVSIPLQLQTLPGTQAQVVTTLPINIGGVTLALPVINNVTAGGGTGQVGQPTTTTDSGTSNGNQLVSTPTTSTAPASTMPESPSSSTTCTTTASTTLTSSDTLVSSADTGQYASTSASSSERTIEEPQTPAATESEAQSSSQLQSNGIQNAQDQSNSLQQVQIVGQPILQQIQIQQPQQQIIQAIPPQSFQLQSGQTIQTIQQQPLQNVQLQAVNPTQVLIRAPTLTPSGQISWQTVQVQNIQSLSNLQVQNAGLSQQLTITPVSSSGGTTLAQIAPVAVAGAPITLNTAQLASVPNLQTVSVANLGAAGVQVQGVPVTITSVAGQQQGQDGVKVQQATIAPVTVAVGGIANATIGAVSPDQLTQVHLQQGQQTSDAEVQPGKRLRRVACSCPNCREGEGRGSSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSVTEVLGSPRIVTVAAISQDSNPATPNVSTNMEEF

Target ORF information:

RefSeq Version	XM_017315007.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus trans-acting transcription factor 4 (Sp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315007.1

ORF Insert Sequence:

ATGGCTACAG AAGGAGGGAA AACCTCTGAG CCAGAGAATA ACAATAAAAA ACCCAAAACC 
TCAGGCTCCC AGGACTCTCA GCCCTCTCCT CTGGCTTTAC TGGCAGCTAC TTGCAGCAAA 
ATAGGGACTC CTGGTGAAAA TCAAGCAACT GGACAACAGC AGATTATTAT AGATCCAAGC 
CAAGGATTGG TGCAACTTCA GAATCAGCCA CAGCAGCTAG AACTGGTGAC GACGCAGCTT 
GCTGGAAACG CTTGGCAACT TGTTGCCTCC ACTCCTCCTG CTTCAAAAGA AAATAACGTT 
TCTCAGCCAG CTTCTAGTTC ATCTAGTTCT TCCAGCAGTA ATAACGGGAG TTCCTCTCCT 
ACAAAAACTA AATCAGGTAA TCCTTCCACC CCTAATCAAT TTCAAGTCAT ACAAGTACAA 
AACCCAAGCG GTAGTGTACA GTACCAAGTG ATCCCCCAAC TTCAGACGGT GGAAGGCCAG 
CAAATTCAAA TCAACCCAAC TAGTAGTTCA TCTCTACAGG ATTTGCAGGG TCAAATTCAG 
CTCATTTCTG CAGGTAATAA TCAAGCTATC CTCACGGCTG CTAACAGGAC AGCTTCTGGG 
AATATCCTTG CTCAAAACCT GGCAAATCAG ACAGTTCCAG TACAAATTAG ACCTGGCGTT 
TCAATACCAT TGCAATTGCA GACCCTTCCT GGTACTCAGG CTCAAGTTGT AACAACCCTA 
CCAATTAACA TTGGAGGAGT GACTTTAGCT TTGCCAGTAA TAAATAACGT GACTGCTGGA 
GGAGGGACTG GACAAGTCGG CCAGCCTACT ACTACTACTG ACAGTGGGAC TTCCAATGGG 
AATCAGTTAG TTTCTACGCC CACCACCAGC ACTGCTCCCG CCAGTACTAT GCCTGAATCG 
CCCTCCTCCT CTACTACCTG CACAACCACC GCATCAACAA CTCTGACGAG CAGTGACACA 
TTAGTGAGCT CAGCAGATAC AGGCCAGTAT GCAAGCACAT CAGCCAGTAG TTCTGAACGC 
ACCATTGAAG AACCACAGAC ACCTGCTGCT ACTGAGTCTG AAGCCCAGAG CTCCAGCCAG 
CTTCAGTCCA ACGGAATTCA GAATGCACAA GACCAGTCAA ATTCCCTTCA ACAAGTACAG 
ATTGTAGGCC AGCCTATCCT GCAGCAAATC CAGATCCAGC AGCCTCAGCA GCAGATTATT 
CAAGCTATCC CACCACAGTC ATTTCAGCTC CAGTCAGGGC AGACAATTCA GACCATCCAG 
CAGCAGCCTT TGCAGAATGT TCAACTTCAA GCAGTAAACC CGACTCAGGT ACTTATCAGG 
GCTCCAACTT TAACACCTTC AGGGCAAATC AGCTGGCAAA CTGTCCAGGT CCAGAATATT 
CAAAGTCTTT CAAATTTGCA AGTTCAGAAT GCTGGGTTAT CCCAGCAGTT AACCATCACC 
CCAGTGTCTT CAAGTGGTGG CACAACTCTT GCTCAGATTG CTCCTGTGGC TGTTGCTGGT 
GCCCCAATAA CTTTGAATAC TGCCCAGCTT GCATCAGTGC CTAACCTTCA GACAGTGAGC 
GTTGCCAACC TGGGTGCTGC GGGTGTTCAA GTTCAGGGAG TTCCAGTAAC AATCACTAGT 
GTTGCAGGTC AACAGCAAGG ACAGGATGGA GTGAAAGTCC AACAAGCTAC TATAGCTCCT 
GTAACGGTGG CAGTTGGAGG GATTGCTAAT GCGACAATAG GTGCTGTTAG TCCTGACCAA 
CTCACACAAG TGCATTTGCA GCAAGGCCAG CAGACCTCTG ATGCAGAGGT GCAACCTGGC 
AAGAGGCTCC GAAGAGTTGC CTGCTCCTGT CCTAACTGTA GGGAAGGAGA AGGAAGGGGC 
AGCAGTGAAC CAGGAAAGAA GAAGCAGCAT GTTTGCCACA TTGAAGGGTG TGGGAAAGTA 
TATGGCAAGA CCTCTCACCT CCGAGCACAT CTCCGATGGC ACACTGGAGA AAGACCGTTC 
ATATGCAACT GGATGTTTTG TGGCAAAAGA TTCACAAGGA GCGATGAGCT TCAGAGACAC 
AGAAGGACCC ACACAGGTGA AAAGAGATTT GAATGTCCAG AGTGTTCTAA AAGGTTTATG 
CGGAGTGATC ACCTATCCAA GCATGTCAAA ACTCACCAGA ATAAGAAAGG AGGTGGGACA 
GCTCTTGCCA TTGTTACCTC CGGAGAACTG GACTCATCTG TTACAGAGGT GCTTGGCTCT 
CCACGGATTG TCACGGTTGC CGCCATTTCC CAAGATTCGA ATCCAGCAAC TCCCAATGTT 
TCAACAAACA TGGAAGAATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sp2 is the only glutamine-rich specificity protein with minor impact on development and differentiation in myelinating glia.
Journal of neurochemistry140(2)245-256(2017 Jan)
Wegener A,K?spert M,Sock E,Philipsen S,Suske G,Wegner M

Restoration of Sp4 in Forebrain GABAergic Neurons Rescues Hypersensitivity to Ketamine in Sp4 Hypomorphic Mice.
The international journal of neuropsychopharmacology18(11)pyv063(2015 Jun)
Higa KK,Ji B,Buell MR,Risbrough VB,Powell SB,Young JW,Geyer MA,Zhou X

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Sp4 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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