Sp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sp1
Entrez Gene ID	20683
Full Name	trans-acting transcription factor 1
Synonyms	1110003E12Rik,AA450830,AI845540,Sp1-1
General protein information	Preferred Names trans-acting transcription factor 1 Names transcription factor Sp1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 F3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017316523.1	XP_017172012.1	transcription factor Sp1 isoform X1
NM_013672.2	NP_038700.2	transcription factor Sp1

Pathways from BioSystems

Homologies

	SP1	XP_001104948.1
Danio rerio (zebrafish)	sp1	NP_997827.1
Pan troglodytes (chimpanzee)	SP1	XP_003313713.1
Mus musculus (house mouse)	Sp1	NP_038700.2
Xenopus tropicalis (tropical clawed frog)	sp1	NP_989139.1
Homo sapiens (human)	SP1	NP_612482.2
Bos taurus (cattle)	SP1	NP_001071495.1
Gallus gallus (chicken)	SP1	NP_989935.1
Canis lupus familiaris (dog)	SP1	XP_005636828.1
Rattus norvegicus (Norway rat)	Sp1	NP_036787.2

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in neuroblastoma cells.
Hasegawa S, Imai M, Yamasaki M, Takahashi N, Fukui T
Biochemical and biophysical research communications495(1)652-658(2018 01)

Sp-1 Negatively Regulates miR-20b Expression in Macrophages.
Guo S, Wang H, Luo Y, He J, Ma H, Shen L, Tang J, Fang Q, Wang H, Qian Z, Song C
Annals of clinical and laboratory science47(6)706-712(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that Sp1 regulates gene expression of AACS in Neuro-2a cells and ketone body utilization affects the balance of histone acetylation.
Title: Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in neuroblastoma cells.

Using MA, we demonstrated Sp1 as a critical stemness-related transcriptional factor protecting GBM cells against stress- and TMZ-induced death. Thus, Sp1 inhibition may prevent recurrence of malignant cells persisting after primary therapy.
Title: Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.

Results suggest that 25-hydroxycholesterol (25-HC) induced the interaction between NFATc1 and Sp1, reducing the level of free Sp1 to inhibit microRNA miR-139-5p expression and promote osteoclastogenesis.
Title: 25-hydroxycholesterol promotes RANKL-induced osteoclastogenesis through coordinating NFATc1 and Sp1 complex in the transcription of miR-139-5p.

adrenergic stimulation induced complex formation between Ifrd1, Sp1 and mSIN3B, which is a component of the SIN complex containing histone deacetylase, in brown adipocytes. These findings, therefore, suggest that Ifrd1 could be a pivotal negative regulator of sympathetic regulation of thermogenic and mitochondrial gene expression in brown adipocytes by interacting with Sp1 and the mSIN3 complex.
Title: The transcriptional modulator Ifrd1 controls PGC-1? expression under short-term adrenergic stimulation in brown adipocytes.

data indicated that (-)-Epicatechin inhibited AngII-induced cardiac hypertrophy by activating the SP1/SIRT1 signaling pathway.
Title: (-)-Epicatechin Suppresses Angiotensin II-induced Cardiac Hypertrophy via the Activation of the SP1/SIRT1 Signaling Pathway.

The following Sp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22119	NM_013672.2 Latest version!	Mus musculus trans-acting transcription factor 1 (Sp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu68769	XM_017316523.1 Latest version!	Mus musculus trans-acting transcription factor 1 (Sp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sp1 ( NM_013672.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038700.2 Mus musculus trans-acting transcription factor 1 (Sp1), mRNA.

Price & Availability↑

CloneID	OMu22119
Clone ID Related Accession (Same CDS sequence)	NM_013672.2
Accession Version	NM_013672.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	transcription factor Sp1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ181598.1, AF062566.1, CF729889.1 and AC137156.3. On Dec 9, 2006 this sequence version replaced NM_013672.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF022363.1, AF062566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGACC AAGATCACTC CATGGATGAA GTGACAGCTG TGGTGAAGAT TGAAAAAGAT 
GTTGGTGGCA ATAATGGGGG TAGCGGCAAT GGTGGCGGTG CCGCCTTTTC TCAGACTCGA 
AGCAGCAGCA CAGGCAGTAG CAGCAGCAGT GGTGGCGGAG GAGGGCAGGA ATCCCAGCCA 
TCTCCTTTGG CTCTGCTGGC AGCAACCTGC AGCAGAATTG AGTCACCCAA TGAGAACAGC 
AACAACTCCC AGGGTCCGAG TCAGTCAGGG GGCACAGGTG AACTTGACCT CACAGCCGCA 
CAACTTTCAC AGGGTGCCAA TGGCTGGCAG ATCATCTCTT CCTCCTCTGG GGCTACCCCT 
ACCTCAAAGG AACAGAGTGG CAACAGTACC AATGGCAGCG AGTCTTCCAA GAACCGCACA 
GTCTCTGGTG GGCAGTATGT TGTGGCTGCT ACCCCCAACT TACAGAACCA GCAAGTTCTG 
ACAGGTCTCC CTGGAGTAAT GCCTAATATT CAGTATCAAG TAATCCCACA GTTCCAGACT 
GTTGATGGGC AGCAGCTGCA GTTTGCTGCC ACTGGGGCCC AAGTGCAGCA GGATGGTTCT 
GGTCAAATAC AGATCATACC AGGTGCAAAC CAACAGATCA TCCCAAATAG AGGAAGTGGG 
GGCAACATTA TTGCTGCTAT GCCAAATCTA CTCCAGCAGG CTGTCCCCCT TCAAGGCCTT 
GCTAATAATG TGCTCTCAGG ACAGACTCAG TATGTGACCA ATGTACCAGT GGCCCTGAAT 
GGGAACATCA CCTTGCTACC TGTCAACAGC GTTTCTGCAG CTACCCTGAC TCCCAGCTCT 
CAGGCAGGCA CTATCAGCAG CTCTGGATCC CAGGAGAGCA GCTCACAGCC TGTCACCTCA 
GGGACTGCCA TCAGTTCTGC CAGCTTGGTG TCATCACAAG CTAGTTCCAG CTCCTTTTTT 
ACCAATGCCA ATAGTTATTC AACAACTACT ACCACCAGCA ACATGGGAAT TATGAACTTT 
ACCAGCAGTG GCTCATCAGG GACTAGTTCT CAAGGCCAGA CGCCCCAGAG GGTTGGTGGG 
CTACAAGGGT CTGATTCTCT GAACATCCAG CAGAACCAGA CATCAGGAGG CTCGCTGCAA 
GGAAGTCAGC AGAAAGAGGG AGAGCAAAGT CAGCAGACAC AGCAACAACA AATCCTTATT 
CAGCCTCAGC TAGTTCAAGG AGGACAAGCT CTTCAGGCCC TTCAAGCAGC ACCATTGTCC 
GGACAGACCT TCACAACTCA AGCTATTTCC CAGGAAACCC TTCAGAACCT CCAGCTTCAG 
GCTGTTCAAA ACTCTGGTCC CATCATCATT CGGACACCAA CCGTGGGGCC CAATGGACAG 
GTCAGTTGGC AGACCCTTCA GCTGCAGAAT CTTCAAGTTC AGAACCCACA AGCCCAGACA 
ATCACCTTGG CCCCTATGCA GGGTGTTTCC TTGGGGCAGA CTAGCAGCAG TAATACCACC 
CTAACACCCA TTGCCTCAGC TGCCTCCATT CCTGCTGGCA CAGTCACTGT GAATGCTGCT 
CAACTCTCCT CCATGCCAGG CCTCCAGACC ATTAACCTCA GTGCATTGGG TACTTCAGGG 
ATCCAGGTGC ACCAGCTTCC AGGCCTGCCT TTGGCTATAG CAAACACCCC AGGTGATCAT 
GGAACTCAAC TTGGTCTTCA TGGATCTGGT GGTGATGGGA TACATGATGA GACAGCAGGT 
GGAGAAGGAG AGAATAGCTC TGATCTCCAA CCCCAAGCTG GACGCAGGAC TCGTCGGGAA 
GCATGTACAT GCCCCTATTG CAAAGACAGT GAGGGAAGAG CCTCAGGAGA TCCTGGCAAA 
AAGAAACAGC ACATTTGTCA CATCCAAGGA TGCGGCAAAG TATATGGCAA GACCTCACAT 
CTCCGAGCAC ACTTGCGCTG GCATACAGGG GAGAGGCCAT TCATGTGTAA TTGGTCATAT 
TGTGGGAAGC GCTTTACACG TTCGGACGAG CTTCAGAGAC ATAAACGTAC ACATACAGGA 
GAGAAGAAAT TTGCCTGCCC TGAGTGCCCT AAGCGTTTCA TGAGGAGTGA TCACCTGTCA 
AAGCATATCA AGACTCACCA GAACAAGAAG GGAGGCCCAG GTGTAGCCCT GAGTGTGGGC 
ACATTGCCCC TGGACAGTGG GGCAGGTTCA GAAGGCACTG CCACTCCTTC AGCCCTTATT 
ACCACCAATA TGGTAGCCAT GGAGGCCATC TGTCCAGAGG GTATTGCCCG TCTTGCCAAC 
AGTGGCATCA ACGTCATGCA GGTGACAGAG CTGCAGTCCA TTAATATCAG TGGCAATGGT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038700.2
CDS	101..2446
Translation	MSDQDHSMDEVTAVVKIEKDVGGNNGGSGNGGGAAFSQTRSSSTGSSSSSGGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTAAQLSQGANGWQIISSSSGATPTSKEQSGNSTNGSESSKNRTVSGGQYVVAATPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIIPNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAGTISSSGSQESSSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTSSGSSGTSSQGQTPQRVGGLQGSDSLNIQQNQTSGGSLQGSQQKEGEQSQQTQQQQILIQPQLVQGGQALQALQAAPLSGQTFTTQAISQETLQNLQLQAVQNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHQLPGLPLAIANTPGDHGTQLGLHGSGGDGIHDETAGGEGENSSDLQPQAGRRTRREACTCPYCKDSEGRASGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVTELQSINISGNGF

Target ORF information:

RefSeq Version	NM_013672.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus trans-acting transcription factor 1 (Sp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013672.2

ORF Insert Sequence:

ATGAGCGACC AAGATCACTC CATGGATGAA GTGACAGCTG TGGTGAAGAT TGAAAAAGAT 
GTTGGTGGCA ATAATGGGGG TAGCGGCAAT GGTGGCGGTG CCGCCTTTTC TCAGACTCGA 
AGCAGCAGCA CAGGCAGTAG CAGCAGCAGT GGTGGCGGAG GAGGGCAGGA ATCCCAGCCA 
TCTCCTTTGG CTCTGCTGGC AGCAACCTGC AGCAGAATTG AGTCACCCAA TGAGAACAGC 
AACAACTCCC AGGGTCCGAG TCAGTCAGGG GGCACAGGTG AACTTGACCT CACAGCCGCA 
CAACTTTCAC AGGGTGCCAA TGGCTGGCAG ATCATCTCTT CCTCCTCTGG GGCTACCCCT 
ACCTCAAAGG AACAGAGTGG CAACAGTACC AATGGCAGCG AGTCTTCCAA GAACCGCACA 
GTCTCTGGTG GGCAGTATGT TGTGGCTGCT ACCCCCAACT TACAGAACCA GCAAGTTCTG 
ACAGGTCTCC CTGGAGTAAT GCCTAATATT CAGTATCAAG TAATCCCACA GTTCCAGACT 
GTTGATGGGC AGCAGCTGCA GTTTGCTGCC ACTGGGGCCC AAGTGCAGCA GGATGGTTCT 
GGTCAAATAC AGATCATACC AGGTGCAAAC CAACAGATCA TCCCAAATAG AGGAAGTGGG 
GGCAACATTA TTGCTGCTAT GCCAAATCTA CTCCAGCAGG CTGTCCCCCT TCAAGGCCTT 
GCTAATAATG TGCTCTCAGG ACAGACTCAG TATGTGACCA ATGTACCAGT GGCCCTGAAT 
GGGAACATCA CCTTGCTACC TGTCAACAGC GTTTCTGCAG CTACCCTGAC TCCCAGCTCT 
CAGGCAGGCA CTATCAGCAG CTCTGGATCC CAGGAGAGCA GCTCACAGCC TGTCACCTCA 
GGGACTGCCA TCAGTTCTGC CAGCTTGGTG TCATCACAAG CTAGTTCCAG CTCCTTTTTT 
ACCAATGCCA ATAGTTATTC AACAACTACT ACCACCAGCA ACATGGGAAT TATGAACTTT 
ACCAGCAGTG GCTCATCAGG GACTAGTTCT CAAGGCCAGA CGCCCCAGAG GGTTGGTGGG 
CTACAAGGGT CTGATTCTCT GAACATCCAG CAGAACCAGA CATCAGGAGG CTCGCTGCAA 
GGAAGTCAGC AGAAAGAGGG AGAGCAAAGT CAGCAGACAC AGCAACAACA AATCCTTATT 
CAGCCTCAGC TAGTTCAAGG AGGACAAGCT CTTCAGGCCC TTCAAGCAGC ACCATTGTCC 
GGACAGACCT TCACAACTCA AGCTATTTCC CAGGAAACCC TTCAGAACCT CCAGCTTCAG 
GCTGTTCAAA ACTCTGGTCC CATCATCATT CGGACACCAA CCGTGGGGCC CAATGGACAG 
GTCAGTTGGC AGACCCTTCA GCTGCAGAAT CTTCAAGTTC AGAACCCACA AGCCCAGACA 
ATCACCTTGG CCCCTATGCA GGGTGTTTCC TTGGGGCAGA CTAGCAGCAG TAATACCACC 
CTAACACCCA TTGCCTCAGC TGCCTCCATT CCTGCTGGCA CAGTCACTGT GAATGCTGCT 
CAACTCTCCT CCATGCCAGG CCTCCAGACC ATTAACCTCA GTGCATTGGG TACTTCAGGG 
ATCCAGGTGC ACCAGCTTCC AGGCCTGCCT TTGGCTATAG CAAACACCCC AGGTGATCAT 
GGAACTCAAC TTGGTCTTCA TGGATCTGGT GGTGATGGGA TACATGATGA GACAGCAGGT 
GGAGAAGGAG AGAATAGCTC TGATCTCCAA CCCCAAGCTG GACGCAGGAC TCGTCGGGAA 
GCATGTACAT GCCCCTATTG CAAAGACAGT GAGGGAAGAG CCTCAGGAGA TCCTGGCAAA 
AAGAAACAGC ACATTTGTCA CATCCAAGGA TGCGGCAAAG TATATGGCAA GACCTCACAT 
CTCCGAGCAC ACTTGCGCTG GCATACAGGG GAGAGGCCAT TCATGTGTAA TTGGTCATAT 
TGTGGGAAGC GCTTTACACG TTCGGACGAG CTTCAGAGAC ATAAACGTAC ACATACAGGA 
GAGAAGAAAT TTGCCTGCCC TGAGTGCCCT AAGCGTTTCA TGAGGAGTGA TCACCTGTCA 
AAGCATATCA AGACTCACCA GAACAAGAAG GGAGGCCCAG GTGTAGCCCT GAGTGTGGGC 
ACATTGCCCC TGGACAGTGG GGCAGGTTCA GAAGGCACTG CCACTCCTTC AGCCCTTATT 
ACCACCAATA TGGTAGCCAT GGAGGCCATC TGTCCAGAGG GTATTGCCCG TCTTGCCAAC 
AGTGGCATCA ACGTCATGCA GGTGACAGAG CTGCAGTCCA TTAATATCAG TGGCAATGGT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sp1 ( XM_017316523.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172012.1 Mus musculus trans-acting transcription factor 1 (Sp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68769
Clone ID Related Accession (Same CDS sequence)	XM_017316523.1
Accession Version	XM_017316523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transcription factor Sp1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG TGACAGCTGT GGTGAAGATT GAAAAAGATG TTGGTGGCAA TAATGGGGGT 
AGCGGCAATG GTGGCGGTGC CGCCTTTTCT CAGACTCGAA GCAGCAGCAC AGGCAGTAGC 
AGCAGCAGTG GTGGCGGAGG AGGGCAGGAA TCCCAGCCAT CTCCTTTGGC TCTGCTGGCA 
GCAACCTGCA GCAGAATTGA GTCACCCAAT GAGAACAGCA ACAACTCCCA GGGTCCGAGT 
CAGTCAGGGG GCACAGGTGA ACTTGACCTC ACAGCCGCAC AACTTTCACA GGGTGCCAAT 
GGCTGGCAGA TCATCTCTTC CTCCTCTGGG GCTACCCCTA CCTCAAAGGA ACAGAGTGGC 
AACAGTACCA ATGGCAGCGA GTCTTCCAAG AACCGCACAG TCTCTGGTGG GCAGTATGTT 
GTGGCTGCTA CCCCCAACTT ACAGAACCAG CAAGTTCTGA CAGGTCTCCC TGGAGTAATG 
CCTAATATTC AGTATCAAGT AATCCCACAG TTCCAGACTG TTGATGGGCA GCAGCTGCAG 
TTTGCTGCCA CTGGGGCCCA AGTGCAGCAG GATGGTTCTG GTCAAATACA GATCATACCA 
GGTGCAAACC AACAGATCAT CCCAAATAGA GGAAGTGGGG GCAACATTAT TGCTGCTATG 
CCAAATCTAC TCCAGCAGGC TGTCCCCCTT CAAGGCCTTG CTAATAATGT GCTCTCAGGA 
CAGACTCAGT ATGTGACCAA TGTACCAGTG GCCCTGAATG GGAACATCAC CTTGCTACCT 
GTCAACAGCG TTTCTGCAGC TACCCTGACT CCCAGCTCTC AGGCAGGCAC TATCAGCAGC 
TCTGGATCCC AGGAGAGCAG CTCACAGCCT GTCACCTCAG GGACTGCCAT CAGTTCTGCC 
AGCTTGGTGT CATCACAAGC TAGTTCCAGC TCCTTTTTTA CCAATGCCAA TAGTTATTCA 
ACAACTACTA CCACCAGCAA CATGGGAATT ATGAACTTTA CCAGCAGTGG CTCATCAGGG 
ACTAGTTCTC AAGGCCAGAC GCCCCAGAGG GTTGGTGGGC TACAAGGGTC TGATTCTCTG 
AACATCCAGC AGAACCAGAC ATCAGGAGGC TCGCTGCAAG GAAGTCAGCA GAAAGAGGGA 
GAGCAAAGTC AGCAGACACA GCAACAACAA ATCCTTATTC AGCCTCAGCT AGTTCAAGGA 
GGACAAGCTC TTCAGGCCCT TCAAGCAGCA CCATTGTCCG GACAGACCTT CACAACTCAA 
GCTATTTCCC AGGAAACCCT TCAGAACCTC CAGCTTCAGG CTGTTCAAAA CTCTGGTCCC 
ATCATCATTC GGACACCAAC CGTGGGGCCC AATGGACAGG TCAGTTGGCA GACCCTTCAG 
CTGCAGAATC TTCAAGTTCA GAACCCACAA GCCCAGACAA TCACCTTGGC CCCTATGCAG 
GGTGTTTCCT TGGGGCAGAC TAGCAGCAGT AATACCACCC TAACACCCAT TGCCTCAGCT 
GCCTCCATTC CTGCTGGCAC AGTCACTGTG AATGCTGCTC AACTCTCCTC CATGCCAGGC 
CTCCAGACCA TTAACCTCAG TGCATTGGGT ACTTCAGGGA TCCAGGTGCA CCAGCTTCCA 
GGCCTGCCTT TGGCTATAGC AAACACCCCA GGTGATCATG GAACTCAACT TGGTCTTCAT 
GGATCTGGTG GTGATGGGAT ACATGATGAG ACAGCAGGTG GAGAAGGAGA GAATAGCTCT 
GATCTCCAAC CCCAAGCTGG ACGCAGGACT CGTCGGGAAG CATGTACATG CCCCTATTGC 
AAAGACAGTG AGGGAAGAGC CTCAGGAGAT CCTGGCAAAA AGAAACAGCA CATTTGTCAC 
ATCCAAGGAT GCGGCAAAGT ATATGGCAAG ACCTCACATC TCCGAGCACA CTTGCGCTGG 
CATACAGGGG AGAGGCCATT CATGTGTAAT TGGTCATATT GTGGGAAGCG CTTTACACGT 
TCGGACGAGC TTCAGAGACA TAAACGTACA CATACAGGAG AGAAGAAATT TGCCTGCCCT 
GAGTGCCCTA AGCGTTTCAT GAGGAGTGAT CACCTGTCAA AGCATATCAA GACTCACCAG 
AACAAGAAGG GAGGCCCAGG TGTAGCCCTG AGTGTGGGCA CATTGCCCCT GGACAGTGGG 
GCAGGTTCAG AAGGCACTGC CACTCCTTCA GCCCTTATTA CCACCAATAT GGTAGCCATG 
GAGGCCATCT GTCCAGAGGG TATTGCCCGT CTTGCCAACA GTGGCATCAA CGTCATGCAG 
GTGACAGAGC TGCAGTCCAT TAATATCAGT GGCAATGGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172012.1
CDS	157..2481
Translation	MDEVTAVVKIEKDVGGNNGGSGNGGGAAFSQTRSSSTGSSSSSGGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTAAQLSQGANGWQIISSSSGATPTSKEQSGNSTNGSESSKNRTVSGGQYVVAATPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIIPNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAGTISSSGSQESSSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTSSGSSGTSSQGQTPQRVGGLQGSDSLNIQQNQTSGGSLQGSQQKEGEQSQQTQQQQILIQPQLVQGGQALQALQAAPLSGQTFTTQAISQETLQNLQLQAVQNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHQLPGLPLAIANTPGDHGTQLGLHGSGGDGIHDETAGGEGENSSDLQPQAGRRTRREACTCPYCKDSEGRASGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVTELQSINISGNGF

Target ORF information:

RefSeq Version	XM_017316523.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus trans-acting transcription factor 1 (Sp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316523.1

ORF Insert Sequence:

ATGGATGAAG TGACAGCTGT GGTGAAGATT GAAAAAGATG TTGGTGGCAA TAATGGGGGT 
AGCGGCAATG GTGGCGGTGC CGCCTTTTCT CAGACTCGAA GCAGCAGCAC AGGCAGTAGC 
AGCAGCAGTG GTGGCGGAGG AGGGCAGGAA TCCCAGCCAT CTCCTTTGGC TCTGCTGGCA 
GCAACCTGCA GCAGAATTGA GTCACCCAAT GAGAACAGCA ACAACTCCCA GGGTCCGAGT 
CAGTCAGGGG GCACAGGTGA ACTTGACCTC ACAGCCGCAC AACTTTCACA GGGTGCCAAT 
GGCTGGCAGA TCATCTCTTC CTCCTCTGGG GCTACCCCTA CCTCAAAGGA ACAGAGTGGC 
AACAGTACCA ATGGCAGCGA GTCTTCCAAG AACCGCACAG TCTCTGGTGG GCAGTATGTT 
GTGGCTGCTA CCCCCAACTT ACAGAACCAG CAAGTTCTGA CAGGTCTCCC TGGAGTAATG 
CCTAATATTC AGTATCAAGT AATCCCACAG TTCCAGACTG TTGATGGGCA GCAGCTGCAG 
TTTGCTGCCA CTGGGGCCCA AGTGCAGCAG GATGGTTCTG GTCAAATACA GATCATACCA 
GGTGCAAACC AACAGATCAT CCCAAATAGA GGAAGTGGGG GCAACATTAT TGCTGCTATG 
CCAAATCTAC TCCAGCAGGC TGTCCCCCTT CAAGGCCTTG CTAATAATGT GCTCTCAGGA 
CAGACTCAGT ATGTGACCAA TGTACCAGTG GCCCTGAATG GGAACATCAC CTTGCTACCT 
GTCAACAGCG TTTCTGCAGC TACCCTGACT CCCAGCTCTC AGGCAGGCAC TATCAGCAGC 
TCTGGATCCC AGGAGAGCAG CTCACAGCCT GTCACCTCAG GGACTGCCAT CAGTTCTGCC 
AGCTTGGTGT CATCACAAGC TAGTTCCAGC TCCTTTTTTA CCAATGCCAA TAGTTATTCA 
ACAACTACTA CCACCAGCAA CATGGGAATT ATGAACTTTA CCAGCAGTGG CTCATCAGGG 
ACTAGTTCTC AAGGCCAGAC GCCCCAGAGG GTTGGTGGGC TACAAGGGTC TGATTCTCTG 
AACATCCAGC AGAACCAGAC ATCAGGAGGC TCGCTGCAAG GAAGTCAGCA GAAAGAGGGA 
GAGCAAAGTC AGCAGACACA GCAACAACAA ATCCTTATTC AGCCTCAGCT AGTTCAAGGA 
GGACAAGCTC TTCAGGCCCT TCAAGCAGCA CCATTGTCCG GACAGACCTT CACAACTCAA 
GCTATTTCCC AGGAAACCCT TCAGAACCTC CAGCTTCAGG CTGTTCAAAA CTCTGGTCCC 
ATCATCATTC GGACACCAAC CGTGGGGCCC AATGGACAGG TCAGTTGGCA GACCCTTCAG 
CTGCAGAATC TTCAAGTTCA GAACCCACAA GCCCAGACAA TCACCTTGGC CCCTATGCAG 
GGTGTTTCCT TGGGGCAGAC TAGCAGCAGT AATACCACCC TAACACCCAT TGCCTCAGCT 
GCCTCCATTC CTGCTGGCAC AGTCACTGTG AATGCTGCTC AACTCTCCTC CATGCCAGGC 
CTCCAGACCA TTAACCTCAG TGCATTGGGT ACTTCAGGGA TCCAGGTGCA CCAGCTTCCA 
GGCCTGCCTT TGGCTATAGC AAACACCCCA GGTGATCATG GAACTCAACT TGGTCTTCAT 
GGATCTGGTG GTGATGGGAT ACATGATGAG ACAGCAGGTG GAGAAGGAGA GAATAGCTCT 
GATCTCCAAC CCCAAGCTGG ACGCAGGACT CGTCGGGAAG CATGTACATG CCCCTATTGC 
AAAGACAGTG AGGGAAGAGC CTCAGGAGAT CCTGGCAAAA AGAAACAGCA CATTTGTCAC 
ATCCAAGGAT GCGGCAAAGT ATATGGCAAG ACCTCACATC TCCGAGCACA CTTGCGCTGG 
CATACAGGGG AGAGGCCATT CATGTGTAAT TGGTCATATT GTGGGAAGCG CTTTACACGT 
TCGGACGAGC TTCAGAGACA TAAACGTACA CATACAGGAG AGAAGAAATT TGCCTGCCCT 
GAGTGCCCTA AGCGTTTCAT GAGGAGTGAT CACCTGTCAA AGCATATCAA GACTCACCAG 
AACAAGAAGG GAGGCCCAGG TGTAGCCCTG AGTGTGGGCA CATTGCCCCT GGACAGTGGG 
GCAGGTTCAG AAGGCACTGC CACTCCTTCA GCCCTTATTA CCACCAATAT GGTAGCCATG 
GAGGCCATCT GTCCAGAGGG TATTGCCCGT CTTGCCAACA GTGGCATCAA CGTCATGCAG 
GTGACAGAGC TGCAGTCCAT TAATATCAGT GGCAATGGTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in neuroblastoma cells.
Biochemical and biophysical research communications495(1)652-658(2018 01)
Hasegawa S,Imai M,Yamasaki M,Takahashi N,Fukui T

Sp-1 Negatively Regulates miR-20b Expression in Macrophages.
Annals of clinical and laboratory science47(6)706-712(2017 Nov)
Guo S,Wang H,Luo Y,He J,Ma H,Shen L,Tang J,Fang Q,Wang H,Qian Z,Song C

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Sp1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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