Sumo3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sumo3
Entrez Gene ID	20610
Full Name	small ubiquitin-like modifier 3
Synonyms	2810014B19Rik,D10Ertd345e,SMT3A,SUMO-3,Smt3h1
General protein information	Preferred Names small ubiquitin-like modifier 3 Names small ubiquitin-related modifier 3 SMT3 (supressor of mif two, 3) homolog 1 SMT3 suppressor of mif two 3 homolog 3 ubiquitin-like protein SMT3A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 C1\|10 39.72 cM
Summary	This gene encodes a member of the small ubiquitin-like modifier family. The encoded protein may regulate a variety of proteins in many pathways via a post-translational modification, known as SUMOylation. This activity may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Disruption of some of these processes has been associated with cerebral ischemia, neural dysfunction, and heart disease. A pseudogene of this gene has been defined on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001301673.1	NP_001288602.1	small ubiquitin-related modifier 3 isoform 4
NM_019929.4	NP_064313.1	small ubiquitin-related modifier 3 isoform 1 precursor
NM_001301672.1	NP_001288601.1	small ubiquitin-related modifier 3 isoform 3
NM_001301671.1	NP_001288600.1	small ubiquitin-related modifier 3 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SUMO3	NP_008867.2
	SUMO3	XP_001115047.1
Bos taurus (cattle)	LOC784603	XP_005216162.1
Rattus norvegicus (Norway rat)	Sumo3	NP_001019466.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000852	XP_316822.4
Xenopus tropicalis (tropical clawed frog)	sumo3	NP_001004853.1
Pan troglodytes (chimpanzee)	SUMO3	XP_003949790.1
Danio rerio (zebrafish)	sumo3b	NP_001002677.2
Bos taurus (cattle)	SUMO3	NP_001069917.1
Mus musculus (house mouse)	Sumo3	NP_064313.1
Gallus gallus (chicken)	SUMO3	NP_001072966.1
Danio rerio (zebrafish)	sumo3a	NP_998289.1
Drosophila melanogaster (fruit fly)	smt3	NP_477411.1

Gene Ontology
Bibliography

Related articles in PubMed

Temporal and SUMO-specific SUMOylation contribute to the dynamics of Polo-like kinase 1 (PLK1) and spindle integrity during mouse oocyte meiosis.
Feitosa WB, Hwang K, Morris PL
Developmental biology(2017 Dec)

Foxp1 regulation of neonatal vocalizations via cortical development.
Usui N, Araujo DJ, Kulkarni A, Co M, Ellegood J, Harper M, Toriumi K, Lerch JP, Konopka G
Genes & development31(20)2039-2055(2017 Oct)

The SUMO2/3 specific E3 ligase ZNF451-1 regulates PML stability.
Koidl S, Eisenhardt N, Fatouros C, Droescher M, Chaugule VK, Pichler A
The international journal of biochemistry & cell biology79478-487(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a regulatory role of ZNF451-1 in fine-tuning physiological PML levels
Title: The SUMO2/3 specific E3 ligase ZNF451-1 regulates PML stability.

Expression levels and not functional differences between SUMO2 and SUMO3 are critical for normal embryogenesis.
Title: SUMO2 is essential while SUMO3 is dispensable for mouse embryonic development.

Results indicate that a functional SUMO1-3 expression is essential for emotionality and cognition
Title: Neuron-specific Sumo1-3 knockdown in mice impairs episodic and fear memories.

The purpose of this study was to perform a comprehensive analysis of the SUMO3-modified proteome regulated by brain ischemia using a novel SUMO transgenic mouse.
Title: Small ubiquitin-like modifier 3-modified proteome regulated by brain ischemia in novel small ubiquitin-like modifier transgenic mice: putative protective proteins/pathways.

Stress-induced phosphorylation of Thr486 in c-Myb by p38 mitogen-activated protein kinases attenuates conjugation of SUMO-2/3.
Title: Stress-induced phosphorylation of Thr486 in c-Myb by p38 mitogen-activated protein kinases attenuates conjugation of SUMO-2/3.

The following Sumo3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sumo3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11692	NM_019929.4 Latest version!	Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu68753	NM_001301671.1 Latest version!	Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu68754	NM_001301672.1 Latest version!	Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu68755	NM_001301673.1 Latest version!	Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sumo3 ( NM_019929.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064313.1 Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11692
Clone ID Related Accession (Same CDS sequence)	NM_019929.4
Accession Version	NM_019929.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	small ubiquitin-related modifier 3 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC153864.5, CJ069985.1 and AK148306.1. On Aug 30, 2014 this sequence version replaced NM_019929.3. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK148306.1, SRR1660817.2995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAAG AGAAGCCCAA GGAGGGTGTG AAGACAGAGA ATGACCACAT CAACCTGAAA 
GTGGCGGGGC AGGATGGCTC GGTGGTACAG TTCAAGATCA AGAGGCACAC CCCACTGAGC 
AAGCTGATGA AGGCCTACTG TGAGAGGCAG GGCTTGTCAA TGAGGCAGAT TCGATTCCGG 
TTTGATGGAC AACCAATCAA TGAAACAGAC ACTCCAGCCC AGCTGGAGAT GGAGGATGAG 
GACACCATTG ATGTATTCCA GCAGCAGACA GGAGGATCAG CCTCCCGAGG GAGCGTCCCC 
ACACCCAACC GTTGTCCTGA CCTGTGCTAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064313.1
CDS	225..557
Translation	MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGSASRGSVPTPNRCPDLCY

Target ORF information:

RefSeq Version	NM_019929.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019929.4

ORF Insert Sequence:

ATGTCGGAAG AGAAGCCCAA GGAGGGTGTG AAGACAGAGA ATGACCACAT CAACCTGAAA 
GTGGCGGGGC AGGATGGCTC GGTGGTACAG TTCAAGATCA AGAGGCACAC CCCACTGAGC 
AAGCTGATGA AGGCCTACTG TGAGAGGCAG GGCTTGTCAA TGAGGCAGAT TCGATTCCGG 
TTTGATGGAC AACCAATCAA TGAAACAGAC ACTCCAGCCC AGCTGGAGAT GGAGGATGAG 
GACACCATTG ATGTATTCCA GCAGCAGACA GGAGGATCAG CCTCCCGAGG GAGCGTCCCC 
ACACCCAACC GTTGTCCTGA CCTGTGCTAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sumo3 ( NM_001301671.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288600.1 Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu68753
Clone ID Related Accession (Same CDS sequence)	NM_001301671.1
Accession Version	NM_001301671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	small ubiquitin-related modifier 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ187500.1, CJ143013.1, CJ159246.1 and AK148306.1. On Aug 30, 2014 this sequence version replaced XM_006513426.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (2) is the same length as isoform 1, but contains a distinct N-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170896.1, AK171169.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCACTG TGTTGGCTCA GGAGGGTGTG AAGACAGAGA ATGACCACAT CAACCTGAAA 
GTGGCGGGGC AGGATGGCTC GGTGGTACAG TTCAAGATCA AGAGGCACAC CCCACTGAGC 
AAGCTGATGA AGGCCTACTG TGAGAGGCAG GGCTTGTCAA TGAGGCAGAT TCGATTCCGG 
TTTGATGGAC AACCAATCAA TGAAACAGAC ACTCCAGCCC AGCTGGAGAT GGAGGATGAG 
GACACCATTG ATGTATTCCA GCAGCAGACA GGAGGATCAG CCTCCCGAGG GAGCGTCCCC 
ACACCCAACC GTTGTCCTGA CCTGTGCTAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288600.1
CDS	351..683
Translation	MTTVLAQEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGSASRGSVPTPNRCPDLCY

Target ORF information:

RefSeq Version	NM_001301671.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301671.1

ORF Insert Sequence:

ATGACCACTG TGTTGGCTCA GGAGGGTGTG AAGACAGAGA ATGACCACAT CAACCTGAAA 
GTGGCGGGGC AGGATGGCTC GGTGGTACAG TTCAAGATCA AGAGGCACAC CCCACTGAGC 
AAGCTGATGA AGGCCTACTG TGAGAGGCAG GGCTTGTCAA TGAGGCAGAT TCGATTCCGG 
TTTGATGGAC AACCAATCAA TGAAACAGAC ACTCCAGCCC AGCTGGAGAT GGAGGATGAG 
GACACCATTG ATGTATTCCA GCAGCAGACA GGAGGATCAG CCTCCCGAGG GAGCGTCCCC 
ACACCCAACC GTTGTCCTGA CCTGTGCTAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sumo3 ( NM_001301672.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288601.1 Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu68754
Clone ID Related Accession (Same CDS sequence)	NM_001301672.1
Accession Version	NM_001301672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	small ubiquitin-related modifier 3 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC153864.5, CJ069985.1 and AK148306.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CX234077.1, BY062557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849378, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAAG AGAAGCCCAA GGAGGGTGTG AAGACAGAGA ATGACCACAT CAACCTGAAA 
GTGGCGGGGC AGGATGGCTC GGTGGTACAG TTCAAGATCA AGAGGCACAC CCCACTGAGC 
AAGCTGATGA AGGCCTACTG TGAGAGGCAG GGCTTGTCAA TGAGGCAGAT TCGATTCCGG 
TTTGATGGAC AACCAATCAA TGAAACAGAC ACTCCAGCCC AGCAGACAGG AGGATCAGCC 
TCCCGAGGGA GCGTCCCCAC ACCCAACCGT TGTCCTGACC TGTGCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288601.1
CDS	225..515
Translation	MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQQTGGSASRGSVPTPNRCPDLCY

Target ORF information:

RefSeq Version	NM_001301672.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301672.1

ORF Insert Sequence:

ATGTCGGAAG AGAAGCCCAA GGAGGGTGTG AAGACAGAGA ATGACCACAT CAACCTGAAA 
GTGGCGGGGC AGGATGGCTC GGTGGTACAG TTCAAGATCA AGAGGCACAC CCCACTGAGC 
AAGCTGATGA AGGCCTACTG TGAGAGGCAG GGCTTGTCAA TGAGGCAGAT TCGATTCCGG 
TTTGATGGAC AACCAATCAA TGAAACAGAC ACTCCAGCCC AGCAGACAGG AGGATCAGCC 
TCCCGAGGGA GCGTCCCCAC ACCCAACCGT TGTCCTGACC TGTGCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sumo3 ( NM_001301673.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288602.1 Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu68755
Clone ID Related Accession (Same CDS sequence)	NM_001301673.1
Accession Version	NM_001301673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	small ubiquitin-related modifier 3 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC153864.5, BY085566.1 and AK148306.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a longer protein (isoform 4), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.48973.1, CJ069301.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAAG AGAAGCCCAA GGTAAGCCCG GAGGGTGTGA AGACAGAGAA TGACCACATC 
AACCTGAAAG TGGCGGGGCA GGATGGCTCG GTGGTACAGT TCAAGATCAA GAGGCACACC 
CCACTGAGCA AGCTGATGAA GGCCTACTGT GAGAGGCAGG GCTTGTCAAT GAGGCAGATT 
CGATTCCGGT TTGATGGACA ACCAATCAAT GAAACAGACA CTCCAGCCCA GCTGGAGATG 
GAGGATGAGG ACACCATTGA TGTATTCCAG CAGCAGACAG GAGGATCAGC CTCCCGAGGG 
AGCGTCCCCA CACCCAACCG TTGTCCTGAC CTGTGCTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288602.1
CDS	225..566
Translation	MSEEKPKVSPEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGSASRGSVPTPNRCPDLCY

Target ORF information:

RefSeq Version	NM_001301673.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus small ubiquitin-like modifier 3 (Sumo3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301673.1

ORF Insert Sequence:

ATGTCGGAAG AGAAGCCCAA GGTAAGCCCG GAGGGTGTGA AGACAGAGAA TGACCACATC 
AACCTGAAAG TGGCGGGGCA GGATGGCTCG GTGGTACAGT TCAAGATCAA GAGGCACACC 
CCACTGAGCA AGCTGATGAA GGCCTACTGT GAGAGGCAGG GCTTGTCAAT GAGGCAGATT 
CGATTCCGGT TTGATGGACA ACCAATCAAT GAAACAGACA CTCCAGCCCA GCTGGAGATG 
GAGGATGAGG ACACCATTGA TGTATTCCAG CAGCAGACAG GAGGATCAGC CTCCCGAGGG 
AGCGTCCCCA CACCCAACCG TTGTCCTGAC CTGTGCTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Temporal and SUMO-specific SUMOylation contribute to the dynamics of Polo-like kinase 1 (PLK1) and spindle integrity during mouse oocyte meiosis.
Developmental biology(2017 Dec)
Feitosa WB,Hwang K,Morris PL

Foxp1 regulation of neonatal vocalizations via cortical development.
Genes & development31(20)2039-2055(2017 Oct)
Usui N,Araujo DJ,Kulkarni A,Co M,Ellegood J,Harper M,Toriumi K,Lerch JP,Konopka G

The SUMO2/3 specific E3 ligase ZNF451-1 regulates PML stability.
The international journal of biochemistry & cell biology79478-487(2016 10)
Koidl S,Eisenhardt N,Fatouros C,Droescher M,Chaugule VK,Pichler A

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Sumo3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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