Slc4a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc4a1
Entrez Gene ID	20533
Full Name	solute carrier family 4 (anion exchanger), member 1
Synonyms	Ae1,CD233,Empb3,l11Jus51
General protein information	Preferred Names solute carrier family 4 (anion exchanger), member 1 Names band 3 anion transport protein AE 1 MEB3 anion exchange protein 1 anion exchanger 1 erythrocyte membrane protein band 3 solute carrier family 4 member 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 66.29 cM\|11 D

mRNA and Protein(s)

mRNA	Protein	Name
NM_011403.2	NP_035533.1	band 3 anion transport protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC4A1	NP_001041496.1
Gallus gallus (chicken)	SLC4A1	NP_990853.1
Homo sapiens (human)	SLC4A1	NP_000333.1
Mus musculus (house mouse)	Slc4a1	NP_035533.1
Pan troglodytes (chimpanzee)	SLC4A1	XP_001151353.2
Rattus norvegicus (Norway rat)	Slc4a1	NP_036783.2
Xenopus tropicalis (tropical clawed frog)	slc4a1	XP_002935581.2
Bos taurus (cattle)	SLC4A1	NP_851379.1

Gene Ontology
Bibliography

Related articles in PubMed

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J
Scientific reports7(1)5164(2017 Jul)

Reversible binding of hemoglobin to band 3 constitutes the molecular switch that mediates O2 regulation of erythrocyte properties.
Chu H, McKenna MM, Krump NA, Zheng S, Mendelsohn L, Thein SL, Garrett LJ, Bodine DM, Low PS
Blood128(23)2708-2716(2016 12)

A fate-mapping approach reveals the composite origin of the connecting tubule and alerts on "single-cell"-specific KO model of the distal nephron.
Trepiccione F, Soukaseum C, Iervolino A, Petrillo F, Zacchia M, Schutz G, Eladari D, Capasso G, Hadchouel J
American journal of physiology. Renal physiology311(5)F901-F906(2016 Nov)

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.
Huang HM, Bauer DC, Lelliott PM, Greth A, McMorran BJ, Foote SJ, Burgio G
Scientific reports637197(2016 Nov)

HIRA Is Required for Heart Development and Directly Regulates Tnni2 and Tnnt3.
Dilg D, Saleh RN, Phelps SE, Rose Y, Dupays L, Murphy C, Mohun T, Anderson RH, Scambler PJ, Chapgier AL
PloS one11(8)e0161096(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

properties are controlled by the O2-dependent association of hemoglobin with band 3
Title: Reversible binding of hemoglobin to band 3 constitutes the molecular switch that mediates O2 regulation of erythrocyte properties.

NH4Cl loading elicited adaptive doubling of AE1 mRNA in GPR4+/+, but a 50% less pronounced response in GPR4-/-.
Title: Adaptation by the collecting duct to an exogenous acid load is blunted by deletion of the proton-sensing receptor GPR4.

Activities of AE1 and the sodium pump are coregulated in kidney.
Title: Physical and functional links between anion exchanger-1 and sodium pump.

AE1 is enhanced by acidosis, NaCl, and the combination of DOCA and NaHCO3 in renal collecting ducts.
Title: Regulation of two renal chloride transporters, AE1 and pendrin, by electrolytes and aldosterone.

These results demonstrate that mammalian erythroblast enucleation does not depend on the membrane integrity generated by the ankyrin-band 3 complex.
Title: Ankyrin and band 3 differentially affect expression of membrane glycoproteins but are not required for erythroblast enucleation.

The following Slc4a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc4a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08105	NM_011403.2 Latest version!	Mus musculus solute carrier family 4 (anion exchanger), member 1 (Slc4a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc4a1 ( NM_011403.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035533.1 Mus musculus solute carrier family 4 (anion exchanger), member 1 (Slc4a1), mRNA.

Price & Availability↑

CloneID	OMu08105
Clone ID Related Accession (Same CDS sequence)	NM_011403.2
Accession Version	NM_011403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	band 3 anion transport protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158143.1, BC052419.1 and W75328.1. On or before Apr 18, 2009 this sequence version replaced NM_027792.1, NM_011403.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK157181.1, AK172395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGACA TGCGGGACCA CGAGGAAGTG CTGGAGATCC CAGATCGAGA CAGCGAAGAA 
GAACTGGAGA ACATAATAGG ACAGATAGCA TATAGAGACC TAACCATCCC TGTGACCGAG 
ATGCAGGACC CAGAAGCTCT TCCCACAGAG CAAACAGCCA CAGACTACGT CCCCAGCAGT 
ACCTCCACAC CGCACCCAAG CTCCGGTCAG GTCTATGTGG AGCTTCAGGA ACTGATGATG 
GACCAGAGGA ACCAGGAACT ACAATGGGTG GAGGCAGCGC ACTGGATAGG GCTGGAGGAA 
AACCTTCGAG AGGATGGTGT ATGGGGTCGC CCACATCTAT CTTACCTGAC CTTCTGGAGC 
CTTCTAGAAC TGCAGAAGGT CTTCTCAAAA GGCACCTTCC TTCTGGGTCT GGCAGAGACA 
TCCCTGGCTG GGGTGGCCAA TCACCTTCTA GACTGCTTCA TCTACGAGGA TCAGATTCGG 
CCTCAGGACC GAGAAGAACT GCTCCGGGCT CTGCTACTCA AACGCAGCCA TGCTGAGGAC 
CTAGGGAATC TGGAGGGGGT GAAGCCCGCC GTCCTGACCC GCTCTGGGGG CGCCTCTGAG 
CCTCTGCTTC CTCACCAGCC ATCGCTGGAG ACCCAGCTGT ACTGCGGACA GGCAGAGGGG 
GGCTCAGAAG GACCCTCAAC ATCTGGGACT CTGAAGATCC CCCCAGATTC AGAAACCACA 
CTGGTGCTAG TGGGCCGGGC TAATTTTCTG GAGAAGCCTG TACTGGGCTT CGTGAGACTG 
AAGGAGGCCG TGCCTCTGGA GGACCTGGTG TTGCCAGAGC CTGTGGGCTT CCTTCTTGTT 
CTGCTGGGAC CTGAGGCTCC GCATGTCGAC TATACCCAGC TGGGCAGGGC TGCAGCAACT 
CTCATGACAG AAAGAGTGTT CCGCATTACC GCCTCCATGG CGCATAACCG AGAGGAGCTG 
CTGCGCTCTC TGGAGAGCTT TCTGGACTGT AGCCTGGTGC TGCCGCCCAC AGACGCCCCT 
TCAGAAAAAG CTCTGCTTAA CCTGGTACCG GTGCAGAAGG AGCTGCTTAG GAGGCGGTAC 
CTGCCCAGCC CCGCCAAGCC AGACCCCAAC TTGTACAACA CTCTAGACTT GAACGGGGGA 
AAAGGGGGCC CTGGTGACGA AGACGACCCT CTACGGCGCA CAGGCCGGAT CTTTGGAGGC 
CTGATCCGTG ATATCCGGCG CCGTTATCCT TACTACCTGA GTGACATCAC CGATGCGCTC 
AGTCCCCAGG TGTTGGCTGC TGTCATCTTC ATCTACTTTG CTGCCTTGTC ACCTGCCGTC 
ACCTTCGGCG GCCTCCTGGG AGAAAAGACC CGGAACTTGA TGGGGGTGTC GGAGCTGCTC 
ATCTCCACAG CAGTGCAAGG CATTCTCTTT GCTCTCCTGG GGGCACAGCC CCTGCTTGTG 
CTAGGCTTCT CAGGACCCCT GCTGGTGTTT GAAGAAGCCT TCTTCTCGTT CTGTGAAAGC 
AATAACCTGG AGTATATCGT GGGCCGCGCG TGGATCGGCT TCTGGCTCAT CCTGCTCGTG 
ATGTTGGTGG TGGCCTTTGA AGGCAGCTTC CTCGTCCAAT ACATCTCCCG ATACACCCAG 
GAGATCTTCT CCTTCCTCAT CTCCCTCATC TTCATCTATG AGACTTTCTC CAAGCTGATC 
AAGATTTTCC AGGACTACCC ACTACAACAG ACTTATGCCC CTGTTGTGAT GAAGCCCAAA 
CCTCAGGGCC CTGTGCCCAA CACAGCCCTC TTCTCTCTTG TGCTCATGGC TGGCACCTTC 
CTACTTGCCA TGACGCTACG AAAGTTCAAG AACAGCACCT ACTTCCCTGG CAAGCTGCGT 
AGAGTCATTG GGGACTTCGG GGTCCCCATC TCCATCCTGA TAATGGTCCT GGTGGATTCC 
TTCATCAAGG GCACCTACAC ACAGAAACTC TCCGTGCCTG ATGGCCTCAA AGTGTCCAAC 
TCCTCCGCCC GGGGCTGGGT CATCCACCCT CTGGGGCTGT ATAGACTTTT CCCCACGTGG 
ATGATGTTTG CCTCCGTTCT GCCCGCCCTG CTTGTCTTCA TTCTCATTTT CCTTGAGTCT 
CAGATCACCA CGCTGATTGT CAGTAAACCA GAACGGAAGA TGATCAAGGG CTCTGGCTTC 
CACCTGGACC TGTTGCTGGT CGTTGGCATG GGTGGGGTGG CTGCCCTCTT TGGGATGCCT 
TGGCTCAGTG CCACCACTGT AAGATCTGTT ACCCACGCCA ATGCCCTCAC AGTCATGGGG 
AAGGCCAGTG GTCCAGGGGC TGCAGCCCAG ATCCAAGAGG TCAAGGAACA GCGGATCAGT 
GGGCTCCTGG TCTCTGTGCT TGTGGGACTG TCCATCCTCA TGGAACCTAT CCTGTCCCGC 
ATCCCCCTGG CTGTGCTCTT CGGCATCTTC CTGTACATGG GAGTCACATC CCTCAGTGGC 
ATCCAGCTCT TTGACCGCAT CTTGCTCCTG TTCAAGCCAC CCAAGTACCA CCCGGATGTG 
CCCTTTGTCA AGAGGGTGAA GACCTGGCGC ATGCACCTCT TCACGGGCAT CCAGATCATC 
TGCCTGGCTG TGCTGTGGGT GGTGAAGTCC ACTCCTGCCT CGCTGGCCTT GCCCTTCGTC 
CTCATCCTCA CAGTGCCTCT CCGTCGTCTC ATCCTCCCGC TCATCTTCAG AGAGCTGGAA 
CTCCAGTGTC TGGACGGTGA TGATGCCAAA GTGACCTTTG ACGAGGAGAA TGGCCTGGAT 
GAATATGACG AAGTGCCCAT GCCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035533.1
CDS	294..3083
Translation	MGDMRDHEEVLEIPDRDSEEELENIIGQIAYRDLTIPVTEMQDPEALPTEQTATDYVPSSTSTPHPSSGQVYVELQELMMDQRNQELQWVEAAHWIGLEENLREDGVWGRPHLSYLTFWSLLELQKVFSKGTFLLGLAETSLAGVANHLLDCFIYEDQIRPQDREELLRALLLKRSHAEDLGNLEGVKPAVLTRSGGASEPLLPHQPSLETQLYCGQAEGGSEGPSTSGTLKIPPDSETTLVLVGRANFLEKPVLGFVRLKEAVPLEDLVLPEPVGFLLVLLGPEAPHVDYTQLGRAAATLMTERVFRITASMAHNREELLRSLESFLDCSLVLPPTDAPSEKALLNLVPVQKELLRRRYLPSPAKPDPNLYNTLDLNGGKGGPGDEDDPLRRTGRIFGGLIRDIRRRYPYYLSDITDALSPQVLAAVIFIYFAALSPAVTFGGLLGEKTRNLMGVSELLISTAVQGILFALLGAQPLLVLGFSGPLLVFEEAFFSFCESNNLEYIVGRAWIGFWLILLVMLVVAFEGSFLVQYISRYTQEIFSFLISLIFIYETFSKLIKIFQDYPLQQTYAPVVMKPKPQGPVPNTALFSLVLMAGTFLLAMTLRKFKNSTYFPGKLRRVIGDFGVPISILIMVLVDSFIKGTYTQKLSVPDGLKVSNSSARGWVIHPLGLYRLFPTWMMFASVLPALLVFILIFLESQITTLIVSKPERKMIKGSGFHLDLLLVVGMGGVAALFGMPWLSATTVRSVTHANALTVMGKASGPGAAAQIQEVKEQRISGLLVSVLVGLSILMEPILSRIPLAVLFGIFLYMGVTSLSGIQLFDRILLLFKPPKYHPDVPFVKRVKTWRMHLFTGIQIICLAVLWVVKSTPASLALPFVLILTVPLRRLILPLIFRELELQCLDGDDAKVTFDEENGLDEYDEVPMPV

Target ORF information:

RefSeq Version	NM_011403.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 4 (anion exchanger), member 1 (Slc4a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011403.2

ORF Insert Sequence:

ATGGGGGACA TGCGGGACCA CGAGGAAGTG CTGGAGATCC CAGATCGAGA CAGCGAAGAA 
GAACTGGAGA ACATAATAGG ACAGATAGCA TATAGAGACC TAACCATCCC TGTGACCGAG 
ATGCAGGACC CAGAAGCTCT TCCCACAGAG CAAACAGCCA CAGACTACGT CCCCAGCAGT 
ACCTCCACAC CGCACCCAAG CTCCGGTCAG GTCTATGTGG AGCTTCAGGA ACTGATGATG 
GACCAGAGGA ACCAGGAACT ACAATGGGTG GAGGCAGCGC ACTGGATAGG GCTGGAGGAA 
AACCTTCGAG AGGATGGTGT ATGGGGTCGC CCACATCTAT CTTACCTGAC CTTCTGGAGC 
CTTCTAGAAC TGCAGAAGGT CTTCTCAAAA GGCACCTTCC TTCTGGGTCT GGCAGAGACA 
TCCCTGGCTG GGGTGGCCAA TCACCTTCTA GACTGCTTCA TCTACGAGGA TCAGATTCGG 
CCTCAGGACC GAGAAGAACT GCTCCGGGCT CTGCTACTCA AACGCAGCCA TGCTGAGGAC 
CTAGGGAATC TGGAGGGGGT GAAGCCCGCC GTCCTGACCC GCTCTGGGGG CGCCTCTGAG 
CCTCTGCTTC CTCACCAGCC ATCGCTGGAG ACCCAGCTGT ACTGCGGACA GGCAGAGGGG 
GGCTCAGAAG GACCCTCAAC ATCTGGGACT CTGAAGATCC CCCCAGATTC AGAAACCACA 
CTGGTGCTAG TGGGCCGGGC TAATTTTCTG GAGAAGCCTG TACTGGGCTT CGTGAGACTG 
AAGGAGGCCG TGCCTCTGGA GGACCTGGTG TTGCCAGAGC CTGTGGGCTT CCTTCTTGTT 
CTGCTGGGAC CTGAGGCTCC GCATGTCGAC TATACCCAGC TGGGCAGGGC TGCAGCAACT 
CTCATGACAG AAAGAGTGTT CCGCATTACC GCCTCCATGG CGCATAACCG AGAGGAGCTG 
CTGCGCTCTC TGGAGAGCTT TCTGGACTGT AGCCTGGTGC TGCCGCCCAC AGACGCCCCT 
TCAGAAAAAG CTCTGCTTAA CCTGGTACCG GTGCAGAAGG AGCTGCTTAG GAGGCGGTAC 
CTGCCCAGCC CCGCCAAGCC AGACCCCAAC TTGTACAACA CTCTAGACTT GAACGGGGGA 
AAAGGGGGCC CTGGTGACGA AGACGACCCT CTACGGCGCA CAGGCCGGAT CTTTGGAGGC 
CTGATCCGTG ATATCCGGCG CCGTTATCCT TACTACCTGA GTGACATCAC CGATGCGCTC 
AGTCCCCAGG TGTTGGCTGC TGTCATCTTC ATCTACTTTG CTGCCTTGTC ACCTGCCGTC 
ACCTTCGGCG GCCTCCTGGG AGAAAAGACC CGGAACTTGA TGGGGGTGTC GGAGCTGCTC 
ATCTCCACAG CAGTGCAAGG CATTCTCTTT GCTCTCCTGG GGGCACAGCC CCTGCTTGTG 
CTAGGCTTCT CAGGACCCCT GCTGGTGTTT GAAGAAGCCT TCTTCTCGTT CTGTGAAAGC 
AATAACCTGG AGTATATCGT GGGCCGCGCG TGGATCGGCT TCTGGCTCAT CCTGCTCGTG 
ATGTTGGTGG TGGCCTTTGA AGGCAGCTTC CTCGTCCAAT ACATCTCCCG ATACACCCAG 
GAGATCTTCT CCTTCCTCAT CTCCCTCATC TTCATCTATG AGACTTTCTC CAAGCTGATC 
AAGATTTTCC AGGACTACCC ACTACAACAG ACTTATGCCC CTGTTGTGAT GAAGCCCAAA 
CCTCAGGGCC CTGTGCCCAA CACAGCCCTC TTCTCTCTTG TGCTCATGGC TGGCACCTTC 
CTACTTGCCA TGACGCTACG AAAGTTCAAG AACAGCACCT ACTTCCCTGG CAAGCTGCGT 
AGAGTCATTG GGGACTTCGG GGTCCCCATC TCCATCCTGA TAATGGTCCT GGTGGATTCC 
TTCATCAAGG GCACCTACAC ACAGAAACTC TCCGTGCCTG ATGGCCTCAA AGTGTCCAAC 
TCCTCCGCCC GGGGCTGGGT CATCCACCCT CTGGGGCTGT ATAGACTTTT CCCCACGTGG 
ATGATGTTTG CCTCCGTTCT GCCCGCCCTG CTTGTCTTCA TTCTCATTTT CCTTGAGTCT 
CAGATCACCA CGCTGATTGT CAGTAAACCA GAACGGAAGA TGATCAAGGG CTCTGGCTTC 
CACCTGGACC TGTTGCTGGT CGTTGGCATG GGTGGGGTGG CTGCCCTCTT TGGGATGCCT 
TGGCTCAGTG CCACCACTGT AAGATCTGTT ACCCACGCCA ATGCCCTCAC AGTCATGGGG 
AAGGCCAGTG GTCCAGGGGC TGCAGCCCAG ATCCAAGAGG TCAAGGAACA GCGGATCAGT 
GGGCTCCTGG TCTCTGTGCT TGTGGGACTG TCCATCCTCA TGGAACCTAT CCTGTCCCGC 
ATCCCCCTGG CTGTGCTCTT CGGCATCTTC CTGTACATGG GAGTCACATC CCTCAGTGGC 
ATCCAGCTCT TTGACCGCAT CTTGCTCCTG TTCAAGCCAC CCAAGTACCA CCCGGATGTG 
CCCTTTGTCA AGAGGGTGAA GACCTGGCGC ATGCACCTCT TCACGGGCAT CCAGATCATC 
TGCCTGGCTG TGCTGTGGGT GGTGAAGTCC ACTCCTGCCT CGCTGGCCTT GCCCTTCGTC 
CTCATCCTCA CAGTGCCTCT CCGTCGTCTC ATCCTCCCGC TCATCTTCAG AGAGCTGGAA 
CTCCAGTGTC TGGACGGTGA TGATGCCAAA GTGACCTTTG ACGAGGAGAA TGGCCTGGAT 
GAATATGACG AAGTGCCCAT GCCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Scientific reports7(1)5164(2017 Jul)
Huang YS,Delgadillo LF,Cyr KH,Kingsley PD,An X,McGrath KE,Mohandas N,Conboy JG,Waugh RE,Wan J,Palis J

Reversible binding of hemoglobin to band 3 constitutes the molecular switch that mediates O2 regulation of erythrocyte properties.
Blood128(23)2708-2716(2016 12)
Chu H,McKenna MM,Krump NA,Zheng S,Mendelsohn L,Thein SL,Garrett LJ,Bodine DM,Low PS

A fate-mapping approach reveals the composite origin of the connecting tubule and alerts on "single-cell"-specific KO model of the distal nephron.
American journal of physiology. Renal physiology311(5)F901-F906(2016 Nov)
Trepiccione F,Soukaseum C,Iervolino A,Petrillo F,Zacchia M,Schutz G,Eladari D,Capasso G,Hadchouel J

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.
Scientific reports637197(2016 Nov)
Huang HM,Bauer DC,Lelliott PM,Greth A,McMorran BJ,Foote SJ,Burgio G

HIRA Is Required for Heart Development and Directly Regulates Tnni2 and Tnnt3.
PloS one11(8)e0161096(2016)
Dilg D,Saleh RN,Phelps SE,Rose Y,Dupays L,Murphy C,Mohun T,Anderson RH,Scambler PJ,Chapgier AL

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Slc4a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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