Slc22a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc22a3
Entrez Gene ID	20519
Full Name	solute carrier family 22 (organic cation transporter), member 3
Synonyms	EMT,Oct3,Orct3,Slca22a3
General protein information	Preferred Names solute carrier family 22 (organic cation transporter), member 3 Names solute carrier family 22 member 3 organic cation transporter 3 solute carrier family 22, member 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A1\|17 8.52 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011395.2	NP_035525.1	solute carrier family 22 member 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLC22A3	XP_001152133.1
Homo sapiens (human)	SLC22A3	NP_068812.1
Danio rerio (zebrafish)	zgc:175176	NP_001107932.1
	LOC709454	XP_001097972.2
Bos taurus (cattle)	SLC22A3	XP_002690418.1
Rattus norvegicus (Norway rat)	Slc22a3	NP_062103.1
Gallus gallus (chicken)	SLC22A3	XP_419620.4
Canis lupus familiaris (dog)	SLC22A3	XP_533467.3
Mus musculus (house mouse)	Slc22a3	NP_035525.1

Gene Ontology
Bibliography

Related articles in PubMed

STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula.
Wolfe AD, Rodriguez AM, Downs KM
Developmental biology425(1)44-57(2017 05)

Organic cation transporter 3 (OCT3) is localized to intracellular and surface membranes in select glial and neuronal cells within the basolateral amygdaloid complex of both rats and mice.
Gasser PJ, Hurley MM, Chan J, Pickel VM
Brain structure & function222(4)1913-1928(2017 May)

Involvement of organic cation transporter 3 (Oct3/Slc22a3) in the bioavailability and pharmacokinetics of antidiabetic metformin in mice.
Shirasaka Y, Lee N, Zha W, Wagner D, Wang J
Drug metabolism and pharmacokinetics31(5)385-388(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Metformin undergoes negligible metabolism, these results imply that intestinal absorption of metformin is mediated at least in part by Oct3 in mice.
Title: Involvement of organic cation transporter 3 (Oct3/Slc22a3) in the bioavailability and pharmacokinetics of antidiabetic metformin in mice.

Findings suggest that OCT3 plays an important role in the absorption and elimination of metformin and that the transporter is a critical determinant of metformin bioavailability, clearance, and pharmacologic action.
Title: Targeted disruption of organic cation transporter 3 attenuates the pharmacologic response to metformin.

Study showed that few, if any, 5-HT neurons in the dorsal raphe are resistant to loss of Pet-1 for expression of Slc22a3
Title: Pet-1 Controls Tetrahydrobiopterin Pathway and Slc22a3 Transporter Genes in Serotonin Neurons.

These results demonstrate that OCT3 is responsible for metformin accumulation and secretion in salivary glands.
Title: Taste of a pill: organic cation transporter-3 (OCT3) mediates metformin accumulation and secretion in salivary glands.

Data indicate that the expression of the organic cation transporters Slc22a1, Slc22a2 and Slc22a3 essential for the cellular uptake of metformin was highly suppressed in Tsc1+/-renal tumours.
Title: Renal tumours in a Tsc1+/- mouse model show epigenetic suppression of organic cation transporters Slc22a1, Slc22a2 and Slc22a3, and do not respond to metformin.

The following Slc22a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc22a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13544	NM_011395.2 Latest version!	Mus musculus solute carrier family 22 (organic cation transporter), member 3 (Slc22a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$321.30 $459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc22a3 ( NM_011395.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035525.1 Mus musculus solute carrier family 22 (organic cation transporter), member 3 (Slc22a3), mRNA.

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CloneID	OMu13544
Clone ID Related Accession (Same CDS sequence)	NM_011395.2
Accession Version	NM_011395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	solute carrier family 22 member 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC132106.4 and AC087901.21. On Mar 21, 2007 this sequence version replaced NM_011395.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF078750.1, AF082566.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 11845212 ##RefSeq-Attributes-END##

ATGCCCACGT TCGACCAGGC ACTGAGGAAG GCGGGCGAGT TCGGGCGCTT CCAGCGGCGC 
GTGTTCCTGC TGCTGTGCCT GACGGGTGTC ACCTTCGCCT TCCTCTTCGT CGGTGTGGTC 
TTCCTGGGCA GCCAGCCCGA CTACTATTGG TGTCGCGGGC CGCGCGCCAC CGCGCTGGCC 
GAGCGCTGCG CCTGGAGCCC CGAGGAGGAG TGGAACCTCA CCACACCGGA GCTCCACGTC 
CCAGCTGAGC GCCGCGGCCA AGGCCACTGC CACCGCTACC TGCTGGAAGC CACTAATACC 
AGCTCAGAGC TCAGCTGCGA CCCACTCACT GCCTTCCCCA ACCGCTCCGC GCCTCTGGTG 
TCCTGCAGCG GTGACTGGCG CTATGTGGAG ACCCACTCTA CCATCGTCAG CCAGTTTGAC 
CTTGTCTGCA GCAATGCCTG GATGTTGGAC CTCACCCAAG CCATCCTGAA CCTTGGCTTC 
CTGGCTGGGG CTTTTACCTT GGGCTATGCA GCGGACAGAT ATGGCAGGCT CATCATTTAC 
TTAATATCCT GTTTCGGCGT TGGCATCACA GGAGTCGTGG TGGCATTTGC GCCAAATTTT 
TCTGTGTTTG TGATTTTCCG CTTCCTACAA GGAGTGTTTG GAAAGGGGGC CTGGATGACT 
TGCTTCGTGA TCGTGACAGA AATAGTTGGT TCAAAACAAA GGAGGATTGT GGGAATCGTG 
ATCCAGATGT TCTTCACCCT CGGGATCATT ATTCTTCCTG GGATTGCCTA CTTCACGCCC 
AGCTGGCAGG GCATCCAGCT AGCCATCTCT CTGCCCAGCT TTCTCTTCCT CCTCTATTAC 
TGGGTGGTCC CTGAGTCTCC CCGCTGGCTG ATCACCCGGA AGCAAGGAGA GAAAGCCTTG 
CAGATCCTGA GGCGCGTGGC TAAGTGCAAT GGAAAACACC TCTCATCAAA TTACTCAGAG 
ATCACAGTTA CAGATGAAGA AGTCAGTAAC CCATCCTGTT TAGACCTTGT GAGAACTCCC 
CAAATGAGGA AATGCACGCT CATCCTTATG TTTGCTTGGT TCACGAGCGC CGTGGTGTAC 
CAAGGACTTG TCATGCGCCT GGGACTTATC GGAGGCAACC TCTACATAGA CTTCTTTATC 
TCTGGGCTCG TGGAGCTGCC CGGAGCTCTC TTAATCCTTC TGACCATTGA GCGCCTTGGA 
CGACGCCTTC CCTTTGCGGC AAGCAATATA GTGGCAGGGG TGTCGTGTCT GGTCACTGCA 
TTTTTACCAG AAGGGATACC GTGGCTGAGG ACCACAGTCG CTACCCTGGG AAGACTAGGA 
ATAACCATGG CCTTCGAAAT TGTTTATTTG GTAAATTCAG AGTTGTACCC AACGACATTA 
CGGAACTTTG GGGTTTCACT CTGCTCAGGC TTGTGTGACT TTGGGGGGAT TATAGCCCCG 
TTTCTGCTCT TTCGGCTGGC AGCTATATGG TTAGAACTGC CTCTGATCAT CTTTGGGATC 
CTGGCGTCTG TCTGTGGTGG CCTCGTGATG CTTTTGCCTG AAACGAAGGG CATTGCCTTG 
CCGGAGACGG TGGAAGACGT AGAAAAGCTT GGCAGTTCAC AGTTGCATCA GTGTGGCAGA 
AAAAAGAAAA CCCAGGTTTC TACTTCTGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035525.1
CDS	381..2036
Translation	MPTFDQALRKAGEFGRFQRRVFLLLCLTGVTFAFLFVGVVFLGSQPDYYWCRGPRATALAERCAWSPEEEWNLTTPELHVPAERRGQGHCHRYLLEATNTSSELSCDPLTAFPNRSAPLVSCSGDWRYVETHSTIVSQFDLVCSNAWMLDLTQAILNLGFLAGAFTLGYAADRYGRLIIYLISCFGVGITGVVVAFAPNFSVFVIFRFLQGVFGKGAWMTCFVIVTEIVGSKQRRIVGIVIQMFFTLGIIILPGIAYFTPSWQGIQLAISLPSFLFLLYYWVVPESPRWLITRKQGEKALQILRRVAKCNGKHLSSNYSEITVTDEEVSNPSCLDLVRTPQMRKCTLILMFAWFTSAVVYQGLVMRLGLIGGNLYIDFFISGLVELPGALLILLTIERLGRRLPFAASNIVAGVSCLVTAFLPEGIPWLRTTVATLGRLGITMAFEIVYLVNSELYPTTLRNFGVSLCSGLCDFGGIIAPFLLFRLAAIWLELPLIIFGILASVCGGLVMLLPETKGIALPETVEDVEKLGSSQLHQCGRKKKTQVSTSDV

Target ORF information:

RefSeq Version	NM_011395.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 22 (organic cation transporter), member 3 (Slc22a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011395.2

ORF Insert Sequence:

ATGCCCACGT TCGACCAGGC ACTGAGGAAG GCGGGCGAGT TCGGGCGCTT CCAGCGGCGC 
GTGTTCCTGC TGCTGTGCCT GACGGGTGTC ACCTTCGCCT TCCTCTTCGT CGGTGTGGTC 
TTCCTGGGCA GCCAGCCCGA CTACTATTGG TGTCGCGGGC CGCGCGCCAC CGCGCTGGCC 
GAGCGCTGCG CCTGGAGCCC CGAGGAGGAG TGGAACCTCA CCACACCGGA GCTCCACGTC 
CCAGCTGAGC GCCGCGGCCA AGGCCACTGC CACCGCTACC TGCTGGAAGC CACTAATACC 
AGCTCAGAGC TCAGCTGCGA CCCACTCACT GCCTTCCCCA ACCGCTCCGC GCCTCTGGTG 
TCCTGCAGCG GTGACTGGCG CTATGTGGAG ACCCACTCTA CCATCGTCAG CCAGTTTGAC 
CTTGTCTGCA GCAATGCCTG GATGTTGGAC CTCACCCAAG CCATCCTGAA CCTTGGCTTC 
CTGGCTGGGG CTTTTACCTT GGGCTATGCA GCGGACAGAT ATGGCAGGCT CATCATTTAC 
TTAATATCCT GTTTCGGCGT TGGCATCACA GGAGTCGTGG TGGCATTTGC GCCAAATTTT 
TCTGTGTTTG TGATTTTCCG CTTCCTACAA GGAGTGTTTG GAAAGGGGGC CTGGATGACT 
TGCTTCGTGA TCGTGACAGA AATAGTTGGT TCAAAACAAA GGAGGATTGT GGGAATCGTG 
ATCCAGATGT TCTTCACCCT CGGGATCATT ATTCTTCCTG GGATTGCCTA CTTCACGCCC 
AGCTGGCAGG GCATCCAGCT AGCCATCTCT CTGCCCAGCT TTCTCTTCCT CCTCTATTAC 
TGGGTGGTCC CTGAGTCTCC CCGCTGGCTG ATCACCCGGA AGCAAGGAGA GAAAGCCTTG 
CAGATCCTGA GGCGCGTGGC TAAGTGCAAT GGAAAACACC TCTCATCAAA TTACTCAGAG 
ATCACAGTTA CAGATGAAGA AGTCAGTAAC CCATCCTGTT TAGACCTTGT GAGAACTCCC 
CAAATGAGGA AATGCACGCT CATCCTTATG TTTGCTTGGT TCACGAGCGC CGTGGTGTAC 
CAAGGACTTG TCATGCGCCT GGGACTTATC GGAGGCAACC TCTACATAGA CTTCTTTATC 
TCTGGGCTCG TGGAGCTGCC CGGAGCTCTC TTAATCCTTC TGACCATTGA GCGCCTTGGA 
CGACGCCTTC CCTTTGCGGC AAGCAATATA GTGGCAGGGG TGTCGTGTCT GGTCACTGCA 
TTTTTACCAG AAGGGATACC GTGGCTGAGG ACCACAGTCG CTACCCTGGG AAGACTAGGA 
ATAACCATGG CCTTCGAAAT TGTTTATTTG GTAAATTCAG AGTTGTACCC AACGACATTA 
CGGAACTTTG GGGTTTCACT CTGCTCAGGC TTGTGTGACT TTGGGGGGAT TATAGCCCCG 
TTTCTGCTCT TTCGGCTGGC AGCTATATGG TTAGAACTGC CTCTGATCAT CTTTGGGATC 
CTGGCGTCTG TCTGTGGTGG CCTCGTGATG CTTTTGCCTG AAACGAAGGG CATTGCCTTG 
CCGGAGACGG TGGAAGACGT AGAAAAGCTT GGCAGTTCAC AGTTGCATCA GTGTGGCAGA 
AAAAAGAAAA CCCAGGTTTC TACTTCTGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula.
Developmental biology425(1)44-57(2017 05)
Wolfe AD,Rodriguez AM,Downs KM

Organic cation transporter 3 (OCT3) is localized to intracellular and surface membranes in select glial and neuronal cells within the basolateral amygdaloid complex of both rats and mice.
Brain structure & function222(4)1913-1928(2017 May)
Gasser PJ,Hurley MM,Chan J,Pickel VM

Involvement of organic cation transporter 3 (Oct3/Slc22a3) in the bioavailability and pharmacokinetics of antidiabetic metformin in mice.
Drug metabolism and pharmacokinetics31(5)385-388(2016 Oct)
Shirasaka Y,Lee N,Zha W,Wagner D,Wang J

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Slc22a3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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