Slc12a4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc12a4
Entrez Gene ID	20498
Full Name	solute carrier family 12, member 4
Synonyms	AW546649,KCC1,RBCKCC1
General protein information	Preferred Names solute carrier family 12, member 4 Names solute carrier family 12 member 4 K-Cl Co-transporter-1 K-Cl cotransporter KCC1 electroneutral potassium-chloride cotransporter 1 erythroid K-Cl cotransporter 1 mKCC1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 D3\|8 53.06 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530792.2	XP_006530855.1	solute carrier family 12 member 4 isoform X1
NM_001253804.1	NP_001240733.1	solute carrier family 12 member 4 isoform 1
NM_009195.3	NP_033221.1	solute carrier family 12 member 4 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC12A4	NP_005063.1
Gallus gallus (chicken)	SLC12A4	XP_003641964.1
Danio rerio (zebrafish)	slc12a4	XP_691291.2
Drosophila melanogaster (fruit fly)	kcc	NP_726377.1
Canis lupus familiaris (dog)	SLC12A4	NP_001010952.1
Rattus norvegicus (Norway rat)	Slc12a4	NP_062102.1
Caenorhabditis elegans (roundworm)	kcc-2	NP_001255280.1
Pan troglodytes (chimpanzee)	SLC12A4	XP_003315222.2
	SLC12A4	XP_001096326.2
Bos taurus (cattle)	SLC12A4	NP_001167562.1
Mus musculus (house mouse)	Slc12a4	NP_001240733.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011498	XP_317803.3
Xenopus tropicalis (tropical clawed frog)	LOC100493471	XP_002942716.1

Gene Ontology
Bibliography

Related articles in PubMed

The Zn
Sunuwar L, Asraf H, Donowitz M, Sekler I, Hershfinkel M
Biochimica et biophysica acta1863(4)947-960(2017 Apr)

Functional kinomics establishes a critical node of volume-sensitive cation-Cl
Zhang J, Gao G, Begum G, Wang J, Khanna AR, Shmukler BE, Daubner GM, de Los Heros P, Davies P, Varghese J, Bhuiyan MI, Duan J, Zhang J, Duran D, Alper SL, Sun D, Elledge SJ, Alessi DR, Kahle KT
Scientific reports635986(2016 Oct)

Activation of the erythroid K-Cl cotransporter Kcc1 enhances sickle cell disease pathology in a humanized mouse model.
Brown FC, Conway AJ, Cerruti L, Collinge JE, McLean C, Wiley JS, Kile BT, Jane SM, Curtis DJ
Blood126(26)2863-70(2015 Dec)

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Lumayag S, Haldin CE, Corbett NJ, Wahlin KJ, Cowan C, Turturro S, Larsen PE, Kovacs B, Witmer PD, Valle D, Zack DJ, Nicholson DA, Xu S
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that a single mutant allele of Kcc1 induces widespread sickling and tissue damage, leading to premature death in a humanized mouse model of sickle cell disease
Title: Activation of the erythroid K-Cl cotransporter Kcc1 enhances sickle cell disease pathology in a humanized mouse model.

KCC3 is the dominant isoform in erythrocytes, with variable expression of KCC1 and KCC4 that could result in modulation of KCC activity
Title: K-Cl cotransporter gene expression during human and murine erythroid differentiation.

Treatment of cells expressing mouse K-Cl cotransporter Kcc1 with several chemical cross-linkers shifted Kcc1 polypeptide to higher MW forms. The N-terminal & C-terminal cytoplasmic tails of Kcc1 were not essential for Kcc1 crosslinking.
Title: Chemical crosslinking studies with the mouse Kcc1 K-Cl cotransporter.

Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1) with the cotransporters KCC3, NKCC1, and NKCC2 but not KCC1 and KCC4
Title: Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1).

The following Slc12a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03701	NM_009195.3 Latest version!	Mus musculus solute carrier family 12, member 4 (Slc12a4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu05628	NM_001253804.1 Latest version!	Mus musculus solute carrier family 12, member 4 (Slc12a4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu41304	XM_006530792.2 Latest version!	Mus musculus solute carrier family 12, member 4 (Slc12a4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc12a4 ( NM_009195.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033221.1 Mus musculus solute carrier family 12, member 4 (Slc12a4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu03701
Clone ID Related Accession (Same CDS sequence)	NM_009195.3
Accession Version	NM_009195.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY740705.1 and AK159274.1. On Dec 6, 2011 this sequence version replaced NM_009195.2. Transcript Variant: This variant (2) uses a different splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1 by two amino acids. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK159274.1, AK167371.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCCTCACT TCACCGTGGT GCCGGTGGAC GGGCCGCGAC GCGGCGACTA TGACAACCTC 
GAGGGGCTCA GTTGGGTGGA CTACGGCGAG CGCGCCGAGC GGGAAGACTC GGACGGACAG 
GGTAACCACA GAGAGAATAG CCCTTTCCTT TGCCCTTTGG ACGCCTCCAG AGGAAATGAC 
TACTATGACC GGAACTTGGC ACTGTTTGAG GAGGAGCTGG ACATCCGCCC AAAGGTGTCA 
TCTCTCCTGG GCAAGCTTGT CAGCTATACC AACCTCACCC AAGGAGCCAA GGAGCACGAG 
GAGGCTGAGA GTGGAGAAGG TGGCCGACGG AGAGCTGCCA AGGCTCCCAG CATGGGCACA 
CTCATGGGAG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTCATCCT CTTCCTGCGG 
CTGACCTGGA TGGTGGGCAC AGCTGGTGTG CTGCAGGCTC TCCTCATTGT CCTCATCTGC 
TGCTGCTGTA CCCTGCTGAC AGCCATCTCC ATGAGCGCCA TTGCCACCAA CGGCGTGGTT 
CCAGCTGGTG GCTCGTACTT CATGATTTCC CGCTCTTTGG GACCAGAATT CGGAGGTGCT 
GTGGGCCTGT GCTTCTACCT GGGGACGACA TTTGCAGCAG CCATGTATAT CCTAGGGGCC 
ATTGAGATCT TGCTGACCTA CATTGCTCCG CCAGCTGCCA TCTTTTACCC ATCGGGCACC 
CATGACATGT CAAGCGCCAC CTTGAATAAC ATGAGAGTGT ATGGGACCAT TTTCCTGACT 
CTGATGACCC TAGTGGTGTT TGTCGGTGTC AAGTACGTGA ACAAGTTTGC CTCGCTCTTC 
CTGGCCTGCG TGATCATCTC CATCCTTTCC ATTTACGCGG GAGGCATCAA GTCCATTTTT 
GACCCTCCCG TGTTTCCGGT GTGTATGCTG GGCAATAGGA CCCTGTCTCG GGACCAGTTT 
GACATCTGTG CCAAGACAGT CGTGGTGGAC AATGAGACAG TGGCCACCCG GCTGTGGACT 
TTCTTCTGCC ACAGCCCCAA CCTTACTGCT GACTCCTGTG ACCCCTACTT CCTGCTCAAC 
AATGTGACAG AGATTCCTGG CATACCTGGG GCAGCTGCTG GTGTGCTCCA GGAGAACCTC 
TGGAGCGCTT ACCTGGAGAA GGGGGAGGTC GTGGAGAAGC ATGGGCTGCC GTCCACAGAC 
ACCCTCGGCC TAAAGGAGAG CCTGTCCCTG TATGTGGTGG CTGACATCGC CACGTCCTTC 
ACCGTGCTGG TTGGCATCTT TTTCCCTTCT GTGACAGGCA TCATGGCTGG TTCAAACCGC 
TCCGGGGATC TCCGTGACGC CCAGAAGTCT ATCCCAGTGG GGACCATTCT GGCCATTGTT 
ACCACTTCGC TGGTGTACTT CAGCAGTGTG ATCCTCTTCG GTGCCTGCAT CGAGGGTGTG 
GTACTCCGGG ACAAGTACGG TGATGGCGTC AGCAGGAACC TGGTGGTGGG CACCCTGGCC 
TGGCCTTCGC CTTGGGTCAT CGTGGTTGGC TCCTTCTTCT CAACATGTGG GGCTGGTCTC 
CAGAGCCTCA CTGGGGCGCC ACGTTTACTG CAAGCCATTG CCAAGGATAA CATCATCCCC 
TTCCTCCGGG TGTTTGGCCA TGGGAAAGCA AACGGTGAAC CGACGTGGGC CCTCCTCCTG 
ACAGCGCTCA TCGCTGAGCT GGGCATCCTG ATTGCTTCCC TTGACATGGT GGCCCCCATT 
CTGTCCATGT TCTTTCTGAT GTGTTACCTC TTTGTAAACT TGGCCTGTGC CGTGCAGACA 
CTTCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGAC ATTGTCTTTC 
CTAGGCATGA GTCTCTGCCT GGCTCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTAGTG 
GCCATGCTCA TCGCAGGCAT GATCTACAAG TACATCGAGT ACCAAGGGGC CGAGAAGGAG 
TGGGGCGATG GGATCCGAGG CCTGTCCCTG AGTGCGGCAC GCTATGCACT GCTGAGGCTG 
GAGGAAGGGC CTCCTCACAC CAAGAACTGG CGGCCTCAGC TCCTGGTGCT GCTTAAGTTA 
GACGAAGATC TTCATGTGAA GTACCCCCGG CTCCTCACCT TCGCCTCCCA GCTGAAGGCC 
GGGAAGGGCC TGACAATCGT TGGCTCTGTC ATCCAGGGCA GCTTTCTGGA GAGCTATGGT 
GAAGCCCAGG CCGCTGAGCA GACCATCAAG AACATGATGG ACATTGAGAA AGTCAAAGGC 
TTCTGCCAGG TAGTGGTGGC CAGCAAGGTT CGAGAGGGAC TGGCCCACCT CATCCAGTCT 
TGCGGCCTGG GCGGCATGAG ACACAACTCC GTGGTGCTGG GCTGGCCCTA TGGCTGGCGA 
CAGAGTGAGG ACCCACGTGC CTGGAAGACC TTTATTGACA CTGTGCGCTG CACCACAGCT 
GCCCACCTGG CCCTGCTGGT GCCAAAGAAC ATAGCTTTCT ACCCCAGCAA CCACGAGCGC 
TACCTGGATG GCCATATCGA CGTGTGGTGG ATCGTGCATG ATGGAGGCAT GCTTATGCTG 
CTGCCCTTTC TGCTGCGCCA GCATAAGGTT TGGAAGAAGT GCCGGATGCG CATTTTCACC 
GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ATCTGGCCAT CTTCCTGTAT 
CACCTCCGCC TGGAAGCTGA AGTGGAGGTG GTAGAGATGC ACAACAGTGA CATCTCTGCC 
TATACCTATG AGCGGACACT GATGATGGAG CAGCGGTCTC AGATGCTGCG GCAGATGAGG 
TTGACCAAAA CTGAGCGGGA TCGAGAGGCC CAGCTGGTAA AGGACAGGCA CTCGGCTCTT 
AGGCTGGAGA GCCTCTACTC CGACGAGGAG GAAGAGTCTG TGGCAGGGGC CGACAAGATC 
CAGATGACGT GGACCAGAGA CAAGTACATG GCTGAGCCCT GGGACCCCAG CCACGCCCCT 
GACAACTTCC GGGAGCTGGT GCACATTAAG CCGGACCAGT CCAACGTGCG GCGCATGCAC 
ACTGCTGTGA AGCTCAACGA GGTCATTGTC ACGCGCTCCC ACGATGCCCG TCTGGTTTTG 
CTGAACATGC CTGGCCCCCC TAAGAACAGC GAGGGCGACG AGAACTACAT GGAATTCCTT 
GAAGTCCTAA CCGAGGGCCT TGAACGGGTG TTGTTGGTGC GTGGCGGCGG CCGGGAAGTC 
ATCACCATCT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033221.1
CDS	77..3334
Translation	MPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAEREDSDGQGNHRENSPFLCPLDASRGNDYYDRNLALFEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHERYLDGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTERDREAQLVKDRHSALRLESLYSDEEEESVAGADKIQMTWTRDKYMAEPWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	NM_009195.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 4 (Slc12a4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009195.3

ORF Insert Sequence:

ATGCCTCACT TCACCGTGGT GCCGGTGGAC GGGCCGCGAC GCGGCGACTA TGACAACCTC 
GAGGGGCTCA GTTGGGTGGA CTACGGCGAG CGCGCCGAGC GGGAAGACTC GGACGGACAG 
GGTAACCACA GAGAGAATAG CCCTTTCCTT TGCCCTTTGG ACGCCTCCAG AGGAAATGAC 
TACTATGACC GGAACTTGGC ACTGTTTGAG GAGGAGCTGG ACATCCGCCC AAAGGTGTCA 
TCTCTCCTGG GCAAGCTTGT CAGCTATACC AACCTCACCC AAGGAGCCAA GGAGCACGAG 
GAGGCTGAGA GTGGAGAAGG TGGCCGACGG AGAGCTGCCA AGGCTCCCAG CATGGGCACA 
CTCATGGGAG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTCATCCT CTTCCTGCGG 
CTGACCTGGA TGGTGGGCAC AGCTGGTGTG CTGCAGGCTC TCCTCATTGT CCTCATCTGC 
TGCTGCTGTA CCCTGCTGAC AGCCATCTCC ATGAGCGCCA TTGCCACCAA CGGCGTGGTT 
CCAGCTGGTG GCTCGTACTT CATGATTTCC CGCTCTTTGG GACCAGAATT CGGAGGTGCT 
GTGGGCCTGT GCTTCTACCT GGGGACGACA TTTGCAGCAG CCATGTATAT CCTAGGGGCC 
ATTGAGATCT TGCTGACCTA CATTGCTCCG CCAGCTGCCA TCTTTTACCC ATCGGGCACC 
CATGACATGT CAAGCGCCAC CTTGAATAAC ATGAGAGTGT ATGGGACCAT TTTCCTGACT 
CTGATGACCC TAGTGGTGTT TGTCGGTGTC AAGTACGTGA ACAAGTTTGC CTCGCTCTTC 
CTGGCCTGCG TGATCATCTC CATCCTTTCC ATTTACGCGG GAGGCATCAA GTCCATTTTT 
GACCCTCCCG TGTTTCCGGT GTGTATGCTG GGCAATAGGA CCCTGTCTCG GGACCAGTTT 
GACATCTGTG CCAAGACAGT CGTGGTGGAC AATGAGACAG TGGCCACCCG GCTGTGGACT 
TTCTTCTGCC ACAGCCCCAA CCTTACTGCT GACTCCTGTG ACCCCTACTT CCTGCTCAAC 
AATGTGACAG AGATTCCTGG CATACCTGGG GCAGCTGCTG GTGTGCTCCA GGAGAACCTC 
TGGAGCGCTT ACCTGGAGAA GGGGGAGGTC GTGGAGAAGC ATGGGCTGCC GTCCACAGAC 
ACCCTCGGCC TAAAGGAGAG CCTGTCCCTG TATGTGGTGG CTGACATCGC CACGTCCTTC 
ACCGTGCTGG TTGGCATCTT TTTCCCTTCT GTGACAGGCA TCATGGCTGG TTCAAACCGC 
TCCGGGGATC TCCGTGACGC CCAGAAGTCT ATCCCAGTGG GGACCATTCT GGCCATTGTT 
ACCACTTCGC TGGTGTACTT CAGCAGTGTG ATCCTCTTCG GTGCCTGCAT CGAGGGTGTG 
GTACTCCGGG ACAAGTACGG TGATGGCGTC AGCAGGAACC TGGTGGTGGG CACCCTGGCC 
TGGCCTTCGC CTTGGGTCAT CGTGGTTGGC TCCTTCTTCT CAACATGTGG GGCTGGTCTC 
CAGAGCCTCA CTGGGGCGCC ACGTTTACTG CAAGCCATTG CCAAGGATAA CATCATCCCC 
TTCCTCCGGG TGTTTGGCCA TGGGAAAGCA AACGGTGAAC CGACGTGGGC CCTCCTCCTG 
ACAGCGCTCA TCGCTGAGCT GGGCATCCTG ATTGCTTCCC TTGACATGGT GGCCCCCATT 
CTGTCCATGT TCTTTCTGAT GTGTTACCTC TTTGTAAACT TGGCCTGTGC CGTGCAGACA 
CTTCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGAC ATTGTCTTTC 
CTAGGCATGA GTCTCTGCCT GGCTCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTAGTG 
GCCATGCTCA TCGCAGGCAT GATCTACAAG TACATCGAGT ACCAAGGGGC CGAGAAGGAG 
TGGGGCGATG GGATCCGAGG CCTGTCCCTG AGTGCGGCAC GCTATGCACT GCTGAGGCTG 
GAGGAAGGGC CTCCTCACAC CAAGAACTGG CGGCCTCAGC TCCTGGTGCT GCTTAAGTTA 
GACGAAGATC TTCATGTGAA GTACCCCCGG CTCCTCACCT TCGCCTCCCA GCTGAAGGCC 
GGGAAGGGCC TGACAATCGT TGGCTCTGTC ATCCAGGGCA GCTTTCTGGA GAGCTATGGT 
GAAGCCCAGG CCGCTGAGCA GACCATCAAG AACATGATGG ACATTGAGAA AGTCAAAGGC 
TTCTGCCAGG TAGTGGTGGC CAGCAAGGTT CGAGAGGGAC TGGCCCACCT CATCCAGTCT 
TGCGGCCTGG GCGGCATGAG ACACAACTCC GTGGTGCTGG GCTGGCCCTA TGGCTGGCGA 
CAGAGTGAGG ACCCACGTGC CTGGAAGACC TTTATTGACA CTGTGCGCTG CACCACAGCT 
GCCCACCTGG CCCTGCTGGT GCCAAAGAAC ATAGCTTTCT ACCCCAGCAA CCACGAGCGC 
TACCTGGATG GCCATATCGA CGTGTGGTGG ATCGTGCATG ATGGAGGCAT GCTTATGCTG 
CTGCCCTTTC TGCTGCGCCA GCATAAGGTT TGGAAGAAGT GCCGGATGCG CATTTTCACC 
GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ATCTGGCCAT CTTCCTGTAT 
CACCTCCGCC TGGAAGCTGA AGTGGAGGTG GTAGAGATGC ACAACAGTGA CATCTCTGCC 
TATACCTATG AGCGGACACT GATGATGGAG CAGCGGTCTC AGATGCTGCG GCAGATGAGG 
TTGACCAAAA CTGAGCGGGA TCGAGAGGCC CAGCTGGTAA AGGACAGGCA CTCGGCTCTT 
AGGCTGGAGA GCCTCTACTC CGACGAGGAG GAAGAGTCTG TGGCAGGGGC CGACAAGATC 
CAGATGACGT GGACCAGAGA CAAGTACATG GCTGAGCCCT GGGACCCCAG CCACGCCCCT 
GACAACTTCC GGGAGCTGGT GCACATTAAG CCGGACCAGT CCAACGTGCG GCGCATGCAC 
ACTGCTGTGA AGCTCAACGA GGTCATTGTC ACGCGCTCCC ACGATGCCCG TCTGGTTTTG 
CTGAACATGC CTGGCCCCCC TAAGAACAGC GAGGGCGACG AGAACTACAT GGAATTCCTT 
GAAGTCCTAA CCGAGGGCCT TGAACGGGTG TTGTTGGTGC GTGGCGGCGG CCGGGAAGTC 
ATCACCATCT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a4 ( NM_001253804.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001240733.1 Mus musculus solute carrier family 12, member 4 (Slc12a4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05628
Clone ID Related Accession (Same CDS sequence)	NM_001253804.1
Accession Version	NM_001253804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY740705.1, AK149969.1, AK164479.1 and AK159274.1. Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149969.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCCTCACT TCACCGTGGT GCCGGTGGAC GGGCCGCGAC GCGGCGACTA TGACAACCTC 
GAGGGGCTCA GTTGGGTGGA CTACGGCGAG CGCGCCGAGC GGGAAGACTC GGACGGTGAG 
GGACAGGGTA ACCACAGAGA GAATAGCCCT TTCCTTTGCC CTTTGGACGC CTCCAGAGGA 
AATGACTACT ATGACCGGAA CTTGGCACTG TTTGAGGAGG AGCTGGACAT CCGCCCAAAG 
GTGTCATCTC TCCTGGGCAA GCTTGTCAGC TATACCAACC TCACCCAAGG AGCCAAGGAG 
CACGAGGAGG CTGAGAGTGG AGAAGGTGGC CGACGGAGAG CTGCCAAGGC TCCCAGCATG 
GGCACACTCA TGGGAGTGTA CCTGCCCTGC CTGCAGAATA TCTTTGGGGT CATCCTCTTC 
CTGCGGCTGA CCTGGATGGT GGGCACAGCT GGTGTGCTGC AGGCTCTCCT CATTGTCCTC 
ATCTGCTGCT GCTGTACCCT GCTGACAGCC ATCTCCATGA GCGCCATTGC CACCAACGGC 
GTGGTTCCAG CTGGTGGCTC GTACTTCATG ATTTCCCGCT CTTTGGGACC AGAATTCGGA 
GGTGCTGTGG GCCTGTGCTT CTACCTGGGG ACGACATTTG CAGCAGCCAT GTATATCCTA 
GGGGCCATTG AGATCTTGCT GACCTACATT GCTCCGCCAG CTGCCATCTT TTACCCATCG 
GGCACCCATG ACATGTCAAG CGCCACCTTG AATAACATGA GAGTGTATGG GACCATTTTC 
CTGACTCTGA TGACCCTAGT GGTGTTTGTC GGTGTCAAGT ACGTGAACAA GTTTGCCTCG 
CTCTTCCTGG CCTGCGTGAT CATCTCCATC CTTTCCATTT ACGCGGGAGG CATCAAGTCC 
ATTTTTGACC CTCCCGTGTT TCCGGTGTGT ATGCTGGGCA ATAGGACCCT GTCTCGGGAC 
CAGTTTGACA TCTGTGCCAA GACAGTCGTG GTGGACAATG AGACAGTGGC CACCCGGCTG 
TGGACTTTCT TCTGCCACAG CCCCAACCTT ACTGCTGACT CCTGTGACCC CTACTTCCTG 
CTCAACAATG TGACAGAGAT TCCTGGCATA CCTGGGGCAG CTGCTGGTGT GCTCCAGGAG 
AACCTCTGGA GCGCTTACCT GGAGAAGGGG GAGGTCGTGG AGAAGCATGG GCTGCCGTCC 
ACAGACACCC TCGGCCTAAA GGAGAGCCTG TCCCTGTATG TGGTGGCTGA CATCGCCACG 
TCCTTCACCG TGCTGGTTGG CATCTTTTTC CCTTCTGTGA CAGGCATCAT GGCTGGTTCA 
AACCGCTCCG GGGATCTCCG TGACGCCCAG AAGTCTATCC CAGTGGGGAC CATTCTGGCC 
ATTGTTACCA CTTCGCTGGT GTACTTCAGC AGTGTGATCC TCTTCGGTGC CTGCATCGAG 
GGTGTGGTAC TCCGGGACAA GTACGGTGAT GGCGTCAGCA GGAACCTGGT GGTGGGCACC 
CTGGCCTGGC CTTCGCCTTG GGTCATCGTG GTTGGCTCCT TCTTCTCAAC ATGTGGGGCT 
GGTCTCCAGA GCCTCACTGG GGCGCCACGT TTACTGCAAG CCATTGCCAA GGATAACATC 
ATCCCCTTCC TCCGGGTGTT TGGCCATGGG AAAGCAAACG GTGAACCGAC GTGGGCCCTC 
CTCCTGACAG CGCTCATCGC TGAGCTGGGC ATCCTGATTG CTTCCCTTGA CATGGTGGCC 
CCCATTCTGT CCATGTTCTT TCTGATGTGT TACCTCTTTG TAAACTTGGC CTGTGCCGTG 
CAGACACTTC TGAGGACCCC CAACTGGCGG CCCCGGTTCA AGTACTATCA CTGGACATTG 
TCTTTCCTAG GCATGAGTCT CTGCCTGGCT CTTATGTTTG TCTCCTCCTG GTACTATGCC 
CTAGTGGCCA TGCTCATCGC AGGCATGATC TACAAGTACA TCGAGTACCA AGGGGCCGAG 
AAGGAGTGGG GCGATGGGAT CCGAGGCCTG TCCCTGAGTG CGGCACGCTA TGCACTGCTG 
AGGCTGGAGG AAGGGCCTCC TCACACCAAG AACTGGCGGC CTCAGCTCCT GGTGCTGCTT 
AAGTTAGACG AAGATCTTCA TGTGAAGTAC CCCCGGCTCC TCACCTTCGC CTCCCAGCTG 
AAGGCCGGGA AGGGCCTGAC AATCGTTGGC TCTGTCATCC AGGGCAGCTT TCTGGAGAGC 
TATGGTGAAG CCCAGGCCGC TGAGCAGACC ATCAAGAACA TGATGGACAT TGAGAAAGTC 
AAAGGCTTCT GCCAGGTAGT GGTGGCCAGC AAGGTTCGAG AGGGACTGGC CCACCTCATC 
CAGTCTTGCG GCCTGGGCGG CATGAGACAC AACTCCGTGG TGCTGGGCTG GCCCTATGGC 
TGGCGACAGA GTGAGGACCC ACGTGCCTGG AAGACCTTTA TTGACACTGT GCGCTGCACC 
ACAGCTGCCC ACCTGGCCCT GCTGGTGCCA AAGAACATAG CTTTCTACCC CAGCAACCAC 
GAGCGCTACC TGGATGGCCA TATCGACGTG TGGTGGATCG TGCATGATGG AGGCATGCTT 
ATGCTGCTGC CCTTTCTGCT GCGCCAGCAT AAGGTTTGGA AGAAGTGCCG GATGCGCATT 
TTCACCGTGG CCCAGATGGA TGACAACAGC ATCCAGATGA AGAAGGATCT GGCCATCTTC 
CTGTATCACC TCCGCCTGGA AGCTGAAGTG GAGGTGGTAG AGATGCACAA CAGTGACATC 
TCTGCCTATA CCTATGAGCG GACACTGATG ATGGAGCAGC GGTCTCAGAT GCTGCGGCAG 
ATGAGGTTGA CCAAAACTGA GCGGGATCGA GAGGCCCAGC TGGTAAAGGA CAGGCACTCG 
GCTCTTAGGC TGGAGAGCCT CTACTCCGAC GAGGAGGAAG AGTCTGTGGC AGGGGCCGAC 
AAGATCCAGA TGACGTGGAC CAGAGACAAG TACATGGCTG AGCCCTGGGA CCCCAGCCAC 
GCCCCTGACA ACTTCCGGGA GCTGGTGCAC ATTAAGCCGG ACCAGTCCAA CGTGCGGCGC 
ATGCACACTG CTGTGAAGCT CAACGAGGTC ATTGTCACGC GCTCCCACGA TGCCCGTCTG 
GTTTTGCTGA ACATGCCTGG CCCCCCTAAG AACAGCGAGG GCGACGAGAA CTACATGGAA 
TTCCTTGAAG TCCTAACCGA GGGCCTTGAA CGGGTGTTGT TGGTGCGTGG CGGCGGCCGG 
GAAGTCATCA CCATCTATTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240733.1
CDS	77..3340
Translation	MPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAEREDSDGEGQGNHRENSPFLCPLDASRGNDYYDRNLALFEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHERYLDGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTERDREAQLVKDRHSALRLESLYSDEEEESVAGADKIQMTWTRDKYMAEPWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	NM_001253804.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 4 (Slc12a4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253804.1

ORF Insert Sequence:

ATGCCTCACT TCACCGTGGT GCCGGTGGAC GGGCCGCGAC GCGGCGACTA TGACAACCTC 
GAGGGGCTCA GTTGGGTGGA CTACGGCGAG CGCGCCGAGC GGGAAGACTC GGACGGTGAG 
GGACAGGGTA ACCACAGAGA GAATAGCCCT TTCCTTTGCC CTTTGGACGC CTCCAGAGGA 
AATGACTACT ATGACCGGAA CTTGGCACTG TTTGAGGAGG AGCTGGACAT CCGCCCAAAG 
GTGTCATCTC TCCTGGGCAA GCTTGTCAGC TATACCAACC TCACCCAAGG AGCCAAGGAG 
CACGAGGAGG CTGAGAGTGG AGAAGGTGGC CGACGGAGAG CTGCCAAGGC TCCCAGCATG 
GGCACACTCA TGGGAGTGTA CCTGCCCTGC CTGCAGAATA TCTTTGGGGT CATCCTCTTC 
CTGCGGCTGA CCTGGATGGT GGGCACAGCT GGTGTGCTGC AGGCTCTCCT CATTGTCCTC 
ATCTGCTGCT GCTGTACCCT GCTGACAGCC ATCTCCATGA GCGCCATTGC CACCAACGGC 
GTGGTTCCAG CTGGTGGCTC GTACTTCATG ATTTCCCGCT CTTTGGGACC AGAATTCGGA 
GGTGCTGTGG GCCTGTGCTT CTACCTGGGG ACGACATTTG CAGCAGCCAT GTATATCCTA 
GGGGCCATTG AGATCTTGCT GACCTACATT GCTCCGCCAG CTGCCATCTT TTACCCATCG 
GGCACCCATG ACATGTCAAG CGCCACCTTG AATAACATGA GAGTGTATGG GACCATTTTC 
CTGACTCTGA TGACCCTAGT GGTGTTTGTC GGTGTCAAGT ACGTGAACAA GTTTGCCTCG 
CTCTTCCTGG CCTGCGTGAT CATCTCCATC CTTTCCATTT ACGCGGGAGG CATCAAGTCC 
ATTTTTGACC CTCCCGTGTT TCCGGTGTGT ATGCTGGGCA ATAGGACCCT GTCTCGGGAC 
CAGTTTGACA TCTGTGCCAA GACAGTCGTG GTGGACAATG AGACAGTGGC CACCCGGCTG 
TGGACTTTCT TCTGCCACAG CCCCAACCTT ACTGCTGACT CCTGTGACCC CTACTTCCTG 
CTCAACAATG TGACAGAGAT TCCTGGCATA CCTGGGGCAG CTGCTGGTGT GCTCCAGGAG 
AACCTCTGGA GCGCTTACCT GGAGAAGGGG GAGGTCGTGG AGAAGCATGG GCTGCCGTCC 
ACAGACACCC TCGGCCTAAA GGAGAGCCTG TCCCTGTATG TGGTGGCTGA CATCGCCACG 
TCCTTCACCG TGCTGGTTGG CATCTTTTTC CCTTCTGTGA CAGGCATCAT GGCTGGTTCA 
AACCGCTCCG GGGATCTCCG TGACGCCCAG AAGTCTATCC CAGTGGGGAC CATTCTGGCC 
ATTGTTACCA CTTCGCTGGT GTACTTCAGC AGTGTGATCC TCTTCGGTGC CTGCATCGAG 
GGTGTGGTAC TCCGGGACAA GTACGGTGAT GGCGTCAGCA GGAACCTGGT GGTGGGCACC 
CTGGCCTGGC CTTCGCCTTG GGTCATCGTG GTTGGCTCCT TCTTCTCAAC ATGTGGGGCT 
GGTCTCCAGA GCCTCACTGG GGCGCCACGT TTACTGCAAG CCATTGCCAA GGATAACATC 
ATCCCCTTCC TCCGGGTGTT TGGCCATGGG AAAGCAAACG GTGAACCGAC GTGGGCCCTC 
CTCCTGACAG CGCTCATCGC TGAGCTGGGC ATCCTGATTG CTTCCCTTGA CATGGTGGCC 
CCCATTCTGT CCATGTTCTT TCTGATGTGT TACCTCTTTG TAAACTTGGC CTGTGCCGTG 
CAGACACTTC TGAGGACCCC CAACTGGCGG CCCCGGTTCA AGTACTATCA CTGGACATTG 
TCTTTCCTAG GCATGAGTCT CTGCCTGGCT CTTATGTTTG TCTCCTCCTG GTACTATGCC 
CTAGTGGCCA TGCTCATCGC AGGCATGATC TACAAGTACA TCGAGTACCA AGGGGCCGAG 
AAGGAGTGGG GCGATGGGAT CCGAGGCCTG TCCCTGAGTG CGGCACGCTA TGCACTGCTG 
AGGCTGGAGG AAGGGCCTCC TCACACCAAG AACTGGCGGC CTCAGCTCCT GGTGCTGCTT 
AAGTTAGACG AAGATCTTCA TGTGAAGTAC CCCCGGCTCC TCACCTTCGC CTCCCAGCTG 
AAGGCCGGGA AGGGCCTGAC AATCGTTGGC TCTGTCATCC AGGGCAGCTT TCTGGAGAGC 
TATGGTGAAG CCCAGGCCGC TGAGCAGACC ATCAAGAACA TGATGGACAT TGAGAAAGTC 
AAAGGCTTCT GCCAGGTAGT GGTGGCCAGC AAGGTTCGAG AGGGACTGGC CCACCTCATC 
CAGTCTTGCG GCCTGGGCGG CATGAGACAC AACTCCGTGG TGCTGGGCTG GCCCTATGGC 
TGGCGACAGA GTGAGGACCC ACGTGCCTGG AAGACCTTTA TTGACACTGT GCGCTGCACC 
ACAGCTGCCC ACCTGGCCCT GCTGGTGCCA AAGAACATAG CTTTCTACCC CAGCAACCAC 
GAGCGCTACC TGGATGGCCA TATCGACGTG TGGTGGATCG TGCATGATGG AGGCATGCTT 
ATGCTGCTGC CCTTTCTGCT GCGCCAGCAT AAGGTTTGGA AGAAGTGCCG GATGCGCATT 
TTCACCGTGG CCCAGATGGA TGACAACAGC ATCCAGATGA AGAAGGATCT GGCCATCTTC 
CTGTATCACC TCCGCCTGGA AGCTGAAGTG GAGGTGGTAG AGATGCACAA CAGTGACATC 
TCTGCCTATA CCTATGAGCG GACACTGATG ATGGAGCAGC GGTCTCAGAT GCTGCGGCAG 
ATGAGGTTGA CCAAAACTGA GCGGGATCGA GAGGCCCAGC TGGTAAAGGA CAGGCACTCG 
GCTCTTAGGC TGGAGAGCCT CTACTCCGAC GAGGAGGAAG AGTCTGTGGC AGGGGCCGAC 
AAGATCCAGA TGACGTGGAC CAGAGACAAG TACATGGCTG AGCCCTGGGA CCCCAGCCAC 
GCCCCTGACA ACTTCCGGGA GCTGGTGCAC ATTAAGCCGG ACCAGTCCAA CGTGCGGCGC 
ATGCACACTG CTGTGAAGCT CAACGAGGTC ATTGTCACGC GCTCCCACGA TGCCCGTCTG 
GTTTTGCTGA ACATGCCTGG CCCCCCTAAG AACAGCGAGG GCGACGAGAA CTACATGGAA 
TTCCTTGAAG TCCTAACCGA GGGCCTTGAA CGGGTGTTGT TGGTGCGTGG CGGCGGCCGG 
GAAGTCATCA CCATCTATTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a4 ( XM_006530792.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530855.1 Mus musculus solute carrier family 12, member 4 (Slc12a4), transcript variant X1, mRNA.

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CloneID	OMu41304
Clone ID Related Accession (Same CDS sequence)	XM_006530792.2
Accession Version	XM_006530792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCGG GCGGGGCGGG CCAGCCTGGG GCGGCGGGGA CTGCCGGCTG GAGGCCGGGA 
CCGCGGGCCA AGCGCGCGCG ATGCCTCACT TCACCGTGGT GCCGGTGGAC GGGCCGCGAC 
GCGGCGACTA TGACAACCTC GAGGGGCTCA GTTGGGTGGA CTACGGCGAG CGCGCCGAGC 
GGGAAGACTC GGACGGAGGA GCTGGACATC CGCCCAAAGG TGTCATCTCT CCTGGGCAAG 
CTTGTCAGCT ATACCAACCT CACCCAAGGA GCCAAGGAGC ACGAGGAGGC TGAGAGTGGA 
GAAGGTGGCC GACGGAGAGC TGCCAAGGCT CCCAGCATGG GCACACTCAT GGGAGTGTAC 
CTGCCCTGCC TGCAGAATAT CTTTGGGGTC ATCCTCTTCC TGCGGCTGAC CTGGATGGTG 
GGCACAGCTG GTGTGCTGCA GGCTCTCCTC ATTGTCCTCA TCTGCTGCTG CTGTACCCTG 
CTGACAGCCA TCTCCATGAG CGCCATTGCC ACCAACGGCG TGGTTCCAGC TGGTGGCTCG 
TACTTCATGA TTTCCCGCTC TTTGGGACCA GAATTCGGAG GTGCTGTGGG CCTGTGCTTC 
TACCTGGGGA CGACATTTGC AGCAGCCATG TATATCCTAG GGGCCATTGA GATCTTGCTG 
ACCTACATTG CTCCGCCAGC TGCCATCTTT TACCCATCGG GCACCCATGA CATGTCAAGC 
GCCACCTTGA ATAACATGAG AGTGTATGGG ACCATTTTCC TGACTCTGAT GACCCTAGTG 
GTGTTTGTCG GTGTCAAGTA CGTGAACAAG TTTGCCTCGC TCTTCCTGGC CTGCGTGATC 
ATCTCCATCC TTTCCATTTA CGCGGGAGGC ATCAAGTCCA TTTTTGACCC TCCCGTGTTT 
CCGGTGTGTA TGCTGGGCAA TAGGACCCTG TCTCGGGACC AGTTTGACAT CTGTGCCAAG 
ACAGTCGTGG TGGACAATGA GACAGTGGCC ACCCGGCTGT GGACTTTCTT CTGCCACAGC 
CCCAACCTTA CTGCTGACTC CTGTGACCCC TACTTCCTGC TCAACAATGT GACAGAGATT 
CCTGGCATAC CTGGGGCAGC TGCTGGTGTG CTCCAGGAGA ACCTCTGGAG CGCTTACCTG 
GAGAAGGGGG AGGTCGTGGA GAAGCATGGG CTGCCGTCCA CAGACACCCT CGGCCTAAAG 
GAGAGCCTGT CCCTGTATGT GGTGGCTGAC ATCGCCACGT CCTTCACCGT GCTGGTTGGC 
ATCTTTTTCC CTTCTGTGAC AGGCATCATG GCTGGTTCAA ACCGCTCCGG GGATCTCCGT 
GACGCCCAGA AGTCTATCCC AGTGGGGACC ATTCTGGCCA TTGTTACCAC TTCGCTGGTG 
TACTTCAGCA GTGTGATCCT CTTCGGTGCC TGCATCGAGG GTGTGGTACT CCGGGACAAG 
TACGGTGATG GCGTCAGCAG GAACCTGGTG GTGGGCACCC TGGCCTGGCC TTCGCCTTGG 
GTCATCGTGG TTGGCTCCTT CTTCTCAACA TGTGGGGCTG GTCTCCAGAG CCTCACTGGG 
GCGCCACGTT TACTGCAAGC CATTGCCAAG GATAACATCA TCCCCTTCCT CCGGGTGTTT 
GGCCATGGGA AAGCAAACGG TGAACCGACG TGGGCCCTCC TCCTGACAGC GCTCATCGCT 
GAGCTGGGCA TCCTGATTGC TTCCCTTGAC ATGGTGGCCC CCATTCTGTC CATGTTCTTT 
CTGATGTGTT ACCTCTTTGT AAACTTGGCC TGTGCCGTGC AGACACTTCT GAGGACCCCC 
AACTGGCGGC CCCGGTTCAA GTACTATCAC TGGACATTGT CTTTCCTAGG CATGAGTCTC 
TGCCTGGCTC TTATGTTTGT CTCCTCCTGG TACTATGCCC TAGTGGCCAT GCTCATCGCA 
GGCATGATCT ACAAGTACAT CGAGTACCAA GGGGCCGAGA AGGAGTGGGG CGATGGGATC 
CGAGGCCTGT CCCTGAGTGC GGCACGCTAT GCACTGCTGA GGCTGGAGGA AGGGCCTCCT 
CACACCAAGA ACTGGCGGCC TCAGCTCCTG GTGCTGCTTA AGTTAGACGA AGATCTTCAT 
GTGAAGTACC CCCGGCTCCT CACCTTCGCC TCCCAGCTGA AGGCCGGGAA GGGCCTGACA 
ATCGTTGGCT CTGTCATCCA GGGCAGCTTT CTGGAGAGCT ATGGTGAAGC CCAGGCCGCT 
GAGCAGACCA TCAAGAACAT GATGGACATT GAGAAAGTCA AAGGCTTCTG CCAGGTAGTG 
GTGGCCAGCA AGGTTCGAGA GGGACTGGCC CACCTCATCC AGTCTTGCGG CCTGGGCGGC 
ATGAGACACA ACTCCGTGGT GCTGGGCTGG CCCTATGGCT GGCGACAGAG TGAGGACCCA 
CGTGCCTGGA AGACCTTTAT TGACACTGTG CGCTGCACCA CAGCTGCCCA CCTGGCCCTG 
CTGGTGCCAA AGAACATAGC TTTCTACCCC AGCAACCACG AGCGCTACCT GGATGGCCAT 
ATCGACGTGT GGTGGATCGT GCATGATGGA GGCATGCTTA TGCTGCTGCC CTTTCTGCTG 
CGCCAGCATA AGGTTTGGAA GAAGTGCCGG ATGCGCATTT TCACCGTGGC CCAGATGGAT 
GACAACAGCA TCCAGATGAA GAAGGATCTG GCCATCTTCC TGTATCACCT CCGCCTGGAA 
GCTGAAGTGG AGGTGGTAGA GATGCACAAC AGTGACATCT CTGCCTATAC CTATGAGCGG 
ACACTGATGA TGGAGCAGCG GTCTCAGATG CTGCGGCAGA TGAGGTTGAC CAAAACTGAG 
CGGGATCGAG AGGCCCAGCT GGTAAAGGAC AGGCACTCGG CTCTTAGGCT GGAGAGCCTC 
TACTCCGACG AGGAGGAAGA GTCTGTGGCA GGGGCCGACA AGATCCAGAT GACGTGGACC 
AGAGACAAGT ACATGGCTGA GCCCTGGGAC CCCAGCCACG CCCCTGACAA CTTCCGGGAG 
CTGGTGCACA TTAAGCCGGA CCAGTCCAAC GTGCGGCGCA TGCACACTGC TGTGAAGCTC 
AACGAGGTCA TTGTCACGCG CTCCCACGAT GCCCGTCTGG TTTTGCTGAA CATGCCTGGC 
CCCCCTAAGA ACAGCGAGGG CGACGAGAAC TACATGGAAT TCCTTGAAGT CCTAACCGAG 
GGCCTTGAAC GGGTGTTGTT GGTGCGTGGC GGCGGCCGGG AAGTCATCAC CATCTATTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530855.1
CDS	31..3273
Translation	MRAGGAGQPGAAGTAGWRPGPRAKRARCLTSPWCRWTGRDAATMTTSRGSVGWTTASAPSGKTRTEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHERYLDGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTERDREAQLVKDRHSALRLESLYSDEEEESVAGADKIQMTWTRDKYMAEPWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_006530792.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 4 (Slc12a4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530792.2

ORF Insert Sequence:

ATGCGAGCGG GCGGGGCGGG CCAGCCTGGG GCGGCGGGGA CTGCCGGCTG GAGGCCGGGA 
CCGCGGGCCA AGCGCGCGCG ATGCCTCACT TCACCGTGGT GCCGGTGGAC GGGCCGCGAC 
GCGGCGACTA TGACAACCTC GAGGGGCTCA GTTGGGTGGA CTACGGCGAG CGCGCCGAGC 
GGGAAGACTC GGACGGAGGA GCTGGACATC CGCCCAAAGG TGTCATCTCT CCTGGGCAAG 
CTTGTCAGCT ATACCAACCT CACCCAAGGA GCCAAGGAGC ACGAGGAGGC TGAGAGTGGA 
GAAGGTGGCC GACGGAGAGC TGCCAAGGCT CCCAGCATGG GCACACTCAT GGGAGTGTAC 
CTGCCCTGCC TGCAGAATAT CTTTGGGGTC ATCCTCTTCC TGCGGCTGAC CTGGATGGTG 
GGCACAGCTG GTGTGCTGCA GGCTCTCCTC ATTGTCCTCA TCTGCTGCTG CTGTACCCTG 
CTGACAGCCA TCTCCATGAG CGCCATTGCC ACCAACGGCG TGGTTCCAGC TGGTGGCTCG 
TACTTCATGA TTTCCCGCTC TTTGGGACCA GAATTCGGAG GTGCTGTGGG CCTGTGCTTC 
TACCTGGGGA CGACATTTGC AGCAGCCATG TATATCCTAG GGGCCATTGA GATCTTGCTG 
ACCTACATTG CTCCGCCAGC TGCCATCTTT TACCCATCGG GCACCCATGA CATGTCAAGC 
GCCACCTTGA ATAACATGAG AGTGTATGGG ACCATTTTCC TGACTCTGAT GACCCTAGTG 
GTGTTTGTCG GTGTCAAGTA CGTGAACAAG TTTGCCTCGC TCTTCCTGGC CTGCGTGATC 
ATCTCCATCC TTTCCATTTA CGCGGGAGGC ATCAAGTCCA TTTTTGACCC TCCCGTGTTT 
CCGGTGTGTA TGCTGGGCAA TAGGACCCTG TCTCGGGACC AGTTTGACAT CTGTGCCAAG 
ACAGTCGTGG TGGACAATGA GACAGTGGCC ACCCGGCTGT GGACTTTCTT CTGCCACAGC 
CCCAACCTTA CTGCTGACTC CTGTGACCCC TACTTCCTGC TCAACAATGT GACAGAGATT 
CCTGGCATAC CTGGGGCAGC TGCTGGTGTG CTCCAGGAGA ACCTCTGGAG CGCTTACCTG 
GAGAAGGGGG AGGTCGTGGA GAAGCATGGG CTGCCGTCCA CAGACACCCT CGGCCTAAAG 
GAGAGCCTGT CCCTGTATGT GGTGGCTGAC ATCGCCACGT CCTTCACCGT GCTGGTTGGC 
ATCTTTTTCC CTTCTGTGAC AGGCATCATG GCTGGTTCAA ACCGCTCCGG GGATCTCCGT 
GACGCCCAGA AGTCTATCCC AGTGGGGACC ATTCTGGCCA TTGTTACCAC TTCGCTGGTG 
TACTTCAGCA GTGTGATCCT CTTCGGTGCC TGCATCGAGG GTGTGGTACT CCGGGACAAG 
TACGGTGATG GCGTCAGCAG GAACCTGGTG GTGGGCACCC TGGCCTGGCC TTCGCCTTGG 
GTCATCGTGG TTGGCTCCTT CTTCTCAACA TGTGGGGCTG GTCTCCAGAG CCTCACTGGG 
GCGCCACGTT TACTGCAAGC CATTGCCAAG GATAACATCA TCCCCTTCCT CCGGGTGTTT 
GGCCATGGGA AAGCAAACGG TGAACCGACG TGGGCCCTCC TCCTGACAGC GCTCATCGCT 
GAGCTGGGCA TCCTGATTGC TTCCCTTGAC ATGGTGGCCC CCATTCTGTC CATGTTCTTT 
CTGATGTGTT ACCTCTTTGT AAACTTGGCC TGTGCCGTGC AGACACTTCT GAGGACCCCC 
AACTGGCGGC CCCGGTTCAA GTACTATCAC TGGACATTGT CTTTCCTAGG CATGAGTCTC 
TGCCTGGCTC TTATGTTTGT CTCCTCCTGG TACTATGCCC TAGTGGCCAT GCTCATCGCA 
GGCATGATCT ACAAGTACAT CGAGTACCAA GGGGCCGAGA AGGAGTGGGG CGATGGGATC 
CGAGGCCTGT CCCTGAGTGC GGCACGCTAT GCACTGCTGA GGCTGGAGGA AGGGCCTCCT 
CACACCAAGA ACTGGCGGCC TCAGCTCCTG GTGCTGCTTA AGTTAGACGA AGATCTTCAT 
GTGAAGTACC CCCGGCTCCT CACCTTCGCC TCCCAGCTGA AGGCCGGGAA GGGCCTGACA 
ATCGTTGGCT CTGTCATCCA GGGCAGCTTT CTGGAGAGCT ATGGTGAAGC CCAGGCCGCT 
GAGCAGACCA TCAAGAACAT GATGGACATT GAGAAAGTCA AAGGCTTCTG CCAGGTAGTG 
GTGGCCAGCA AGGTTCGAGA GGGACTGGCC CACCTCATCC AGTCTTGCGG CCTGGGCGGC 
ATGAGACACA ACTCCGTGGT GCTGGGCTGG CCCTATGGCT GGCGACAGAG TGAGGACCCA 
CGTGCCTGGA AGACCTTTAT TGACACTGTG CGCTGCACCA CAGCTGCCCA CCTGGCCCTG 
CTGGTGCCAA AGAACATAGC TTTCTACCCC AGCAACCACG AGCGCTACCT GGATGGCCAT 
ATCGACGTGT GGTGGATCGT GCATGATGGA GGCATGCTTA TGCTGCTGCC CTTTCTGCTG 
CGCCAGCATA AGGTTTGGAA GAAGTGCCGG ATGCGCATTT TCACCGTGGC CCAGATGGAT 
GACAACAGCA TCCAGATGAA GAAGGATCTG GCCATCTTCC TGTATCACCT CCGCCTGGAA 
GCTGAAGTGG AGGTGGTAGA GATGCACAAC AGTGACATCT CTGCCTATAC CTATGAGCGG 
ACACTGATGA TGGAGCAGCG GTCTCAGATG CTGCGGCAGA TGAGGTTGAC CAAAACTGAG 
CGGGATCGAG AGGCCCAGCT GGTAAAGGAC AGGCACTCGG CTCTTAGGCT GGAGAGCCTC 
TACTCCGACG AGGAGGAAGA GTCTGTGGCA GGGGCCGACA AGATCCAGAT GACGTGGACC 
AGAGACAAGT ACATGGCTGA GCCCTGGGAC CCCAGCCACG CCCCTGACAA CTTCCGGGAG 
CTGGTGCACA TTAAGCCGGA CCAGTCCAAC GTGCGGCGCA TGCACACTGC TGTGAAGCTC 
AACGAGGTCA TTGTCACGCG CTCCCACGAT GCCCGTCTGG TTTTGCTGAA CATGCCTGGC 
CCCCCTAAGA ACAGCGAGGG CGACGAGAAC TACATGGAAT TCCTTGAAGT CCTAACCGAG 
GGCCTTGAAC GGGTGTTGTT GGTGCGTGGC GGCGGCCGGG AAGTCATCAC CATCTATTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Zn
Biochimica et biophysica acta1863(4)947-960(2017 Apr)
Sunuwar L,Asraf H,Donowitz M,Sekler I,Hershfinkel M

Functional kinomics establishes a critical node of volume-sensitive cation-Cl
Scientific reports635986(2016 Oct)
Zhang J,Gao G,Begum G,Wang J,Khanna AR,Shmukler BE,Daubner GM,de Los Heros P,Davies P,Varghese J,Bhuiyan MI,Duan J,Zhang J,Duran D,Alper SL,Sun D,Elledge SJ,Alessi DR,Kahle KT

Activation of the erythroid K-Cl cotransporter Kcc1 enhances sickle cell disease pathology in a humanized mouse model.
Blood126(26)2863-70(2015 Dec)
Brown FC,Conway AJ,Cerruti L,Collinge JE,McLean C,Wiley JS,Kile BT,Jane SM,Curtis DJ

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)
Lumayag S,Haldin CE,Corbett NJ,Wahlin KJ,Cowan C,Turturro S,Larsen PE,Kovacs B,Witmer PD,Valle D,Zack DJ,Nicholson DA,Xu S

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Slc12a4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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