Slc12a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc12a3
Entrez Gene ID	20497
Full Name	solute carrier family 12, member 3
Synonyms	AI035291,NCC,NCCT,TSC
General protein information	Preferred Names solute carrier family 12, member 3 Names solute carrier family 12 member 3 Na-Cl symporter thiazide-sensitive sodium-chloride cotransporter
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 C5\|8 46.46 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017312627.1	XP_017168116.1	solute carrier family 12 member 3 isoform X3
NM_001205311.1	NP_001192240.1	solute carrier family 12 member 3 isoform 1
XM_017312626.1	XP_017168115.1	solute carrier family 12 member 3 isoform X2
XM_017312625.1	XP_017168114.1	solute carrier family 12 member 3 isoform X1
NM_019415.2	NP_062288.2	solute carrier family 12 member 3 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc12a3	NP_062288.2
Rattus norvegicus (Norway rat)	Slc12a3	NP_062218.3
Xenopus tropicalis (tropical clawed frog)	slc12a3	XP_002937217.2
Gallus gallus (chicken)	SLC12A3	XP_414059.4
	SLC12A3	XP_001093467.2
Canis lupus familiaris (dog)	SLC12A3	XP_005617594.1
Bos taurus (cattle)	SLC12A3	NP_001193107.1
Danio rerio (zebrafish)	slc12a3	NP_001038545.1
Pan troglodytes (chimpanzee)	SLC12A3	XP_510983.2
Homo sapiens (human)	SLC12A3	NP_000330.2

Gene Ontology
Bibliography

Related articles in PubMed

Potassium intake modulates the thiazide-sensitive sodium-chloride cotransporter (NCC) activity via the Kir4.1 potassium channel.
Wang MX, Cuevas CA, Su XT, Wu P, Gao ZX, Lin DH, McCormick JA, Yang CL, Wang WH, Ellison DH
Kidney international(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

long-term aldosterone administration stimulates mouse NCC and pT58-NCC abundances in late distal convoluted kidney tubules.
Title: Long-term aldosterone administration increases renal Na

Calcineurin is activated by an acute potassium load, which rapidly dephosphorylates NCC, leading to increased urinary potassium excretion.
Title: Calcineurin inhibitors block sodium-chloride cotransporter dephosphorylation in response to?high potassium intake.

Potassium depletion stimulates NCC via phosphorylation and inactivation of the KLHL3 and promoting increased blood pressure.
Title: Potassium depletion stimulates Na-Cl cotransporter

the increased NCC expression and activation is present in CMA which is highly associated with the enhanced WNK4-SPAK signal pathway using WNK4-/- and SPAK-/- mice.
Title: Chronic Metabolic Acidosis Activates Renal Tubular Sodium Chloride Cotransporter through Angiotension II-dependent WNK4-SPAK Phosphorylation Pathway.

The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation.
Title: The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation.

The following Slc12a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu82386	XM_017312627.1 Latest version!	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu20300	NM_001205311.1 Latest version!	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu82384	XM_017312625.1 Latest version!	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu82385	XM_017312626.1 Latest version!	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20583	NM_019415.2 Latest version!	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$587.30 $839.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc12a3 ( XM_017312627.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168116.1 Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu82386
Clone ID Related Accession (Same CDS sequence)	XM_017312627.1
Accession Version	XM_017312627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCA AGGTCGTGAG CTGACTGATG GACTTGTGGA AGATGAGACA 
GGCACCAACA GTGAGAAGAG CCCTGGAGAA CCTGTTCGCT TCGGCTGGGT CAAGGGGGTG 
ATGATCCGTT GCATGCTCAA CATCTGGGGA GTGATCCTCT ACCTGCGACT CCCCTGGATT 
ACGGCGCAGG CGGGCATCGT GCTGACTTGG CTCATCATCC TGCTGTCGGT CATGGTGACA 
TCCATCACCG GCCTCTCCAT CTCCGCCATC TCTACCAATG GCAAGGTCAA GTCGGGTGGC 
ACCTATTTCC TTATTTCCCG GAGTCTGGGA CCAGAGCTGG GAGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCTGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAAACCGTA 
AGGGACCTAC TGCAGCTGGC CTGCTCCCCC ACTCAGGAGT ATGGCACACC CATCGTAGAC 
CCCATCAATG ACATCCGCAT CATCGGTGTG GTCACGGTCA CTGTGCTGCT GGCCATCTCG 
CTGGCTGGGA TGGAGTGGGA GTCCAAGGCC CAGGTGCTAT TCTTTCTTGT CATCATGGTC 
TCCTTTGCCA ACTACCTGGT GGGCACACTG ATCCCAGCAT CTGAAGACAA AGCCTCTAAA 
GGCTTCTACA GCTACCATGG GGACATTTTT GTTCAGAACC TGGTACCCGA CTGGAGAGGC 
ATTGATGGTA GCTTCTTTGG CATGTTCTCC ATCTTCTTCC CTTCGGCCAC TGGCATTCTG 
GCAGGGGCCA ACATCTCCGG GGACCTCAAG GATCCTGCTG TAGCCATCCC CAAGGGAACA 
CTCATGGCCA TTTTCTGGAC CACCATCTCC TACCTTGCCA TCTCAGCTAC CATCGGCTCC 
TGTGTGGTGA GAGATGCCTC TGGGGATGTG AACGACACCA TGACTCCTGG TCCAGGCCCC 
TGCGAAGGGC TAGCCTGTGG CTATGGCTGG AACTTCACAG AATGCTCCCA GCAGCGCAGT 
TGCCGCTATG GCCTCATCAA CTACTACCAG ACCATGAGCA TGGTGTCTGC CTTCGCACCC 
CTGATCACAG CCGGCATCTT TGGCGCCACC CTGTCTTCTG CCTTGGCCTG TCTTGTCTCG 
GCTGCTAAGG TCTTCCAGTG CCTGTGTGAA GACCAGCTGT ACCCTCTGAT TGGCTTTTTT 
GGCAAAGGCT ATGGCAAGAA CAGGGAGCCT GTGCGTGGCT ACCTGCTGGC CTATGCCATT 
GCTGTGGCCT TCATCATCAT TGCTGAACTT AACACCATCG CCCCCATCAT TTCCAACTTC 
TTCCTCTGCT CCTACGCCCT CATCAATTTC AGCTGCTTCC ATGCCTCCAT CACCAACTCA 
CCTGCAAGTG GGCGGCGCTG TTTGGGGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168116.1
CDS	128..1840
Translation	MAELPVTELPGDALCSGRFTISTLMGGDEPPPAACDSSQPSHLTHGSTLYMRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRQGRELTDGLVEDETGTNSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQLACSPTQEYGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASEDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTMTPGPGPCEGLACGYGWNFTECSQQRSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNREPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPASGRRCLGQ

Target ORF information:

RefSeq Version	XM_017312627.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312627.1

ORF Insert Sequence:

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCA AGGTCGTGAG CTGACTGATG GACTTGTGGA AGATGAGACA 
GGCACCAACA GTGAGAAGAG CCCTGGAGAA CCTGTTCGCT TCGGCTGGGT CAAGGGGGTG 
ATGATCCGTT GCATGCTCAA CATCTGGGGA GTGATCCTCT ACCTGCGACT CCCCTGGATT 
ACGGCGCAGG CGGGCATCGT GCTGACTTGG CTCATCATCC TGCTGTCGGT CATGGTGACA 
TCCATCACCG GCCTCTCCAT CTCCGCCATC TCTACCAATG GCAAGGTCAA GTCGGGTGGC 
ACCTATTTCC TTATTTCCCG GAGTCTGGGA CCAGAGCTGG GAGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCTGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAAACCGTA 
AGGGACCTAC TGCAGCTGGC CTGCTCCCCC ACTCAGGAGT ATGGCACACC CATCGTAGAC 
CCCATCAATG ACATCCGCAT CATCGGTGTG GTCACGGTCA CTGTGCTGCT GGCCATCTCG 
CTGGCTGGGA TGGAGTGGGA GTCCAAGGCC CAGGTGCTAT TCTTTCTTGT CATCATGGTC 
TCCTTTGCCA ACTACCTGGT GGGCACACTG ATCCCAGCAT CTGAAGACAA AGCCTCTAAA 
GGCTTCTACA GCTACCATGG GGACATTTTT GTTCAGAACC TGGTACCCGA CTGGAGAGGC 
ATTGATGGTA GCTTCTTTGG CATGTTCTCC ATCTTCTTCC CTTCGGCCAC TGGCATTCTG 
GCAGGGGCCA ACATCTCCGG GGACCTCAAG GATCCTGCTG TAGCCATCCC CAAGGGAACA 
CTCATGGCCA TTTTCTGGAC CACCATCTCC TACCTTGCCA TCTCAGCTAC CATCGGCTCC 
TGTGTGGTGA GAGATGCCTC TGGGGATGTG AACGACACCA TGACTCCTGG TCCAGGCCCC 
TGCGAAGGGC TAGCCTGTGG CTATGGCTGG AACTTCACAG AATGCTCCCA GCAGCGCAGT 
TGCCGCTATG GCCTCATCAA CTACTACCAG ACCATGAGCA TGGTGTCTGC CTTCGCACCC 
CTGATCACAG CCGGCATCTT TGGCGCCACC CTGTCTTCTG CCTTGGCCTG TCTTGTCTCG 
GCTGCTAAGG TCTTCCAGTG CCTGTGTGAA GACCAGCTGT ACCCTCTGAT TGGCTTTTTT 
GGCAAAGGCT ATGGCAAGAA CAGGGAGCCT GTGCGTGGCT ACCTGCTGGC CTATGCCATT 
GCTGTGGCCT TCATCATCAT TGCTGAACTT AACACCATCG CCCCCATCAT TTCCAACTTC 
TTCCTCTGCT CCTACGCCCT CATCAATTTC AGCTGCTTCC ATGCCTCCAT CACCAACTCA 
CCTGCAAGTG GGCGGCGCTG TTTGGGGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( NM_001205311.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001192240.1 Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20300
Clone ID Related Accession (Same CDS sequence)	NM_001205311.1
Accession Version	NM_001205311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052691.1 and AC128663.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCA AGGTCGTGAG CTGACTGATG GACTTGTGGA AGATGAGACA 
GGCACCAACA GTGAGAAGAG CCCTGGAGAA CCTGTTCGCT TCGGCTGGGT CAAGGGGGTG 
ATGATCCGTT GCATGCTCAA CATCTGGGGA GTGATCCTCT ACCTGCGACT CCCCTGGATT 
ACGGCGCAGG CGGGCATCGT GCTGACTTGG CTCATCATCC TGCTGTCGGT CATGGTGACA 
TCCATCACCG GCCTCTCCAT CTCCGCCATC TCTACCAATG GCAAGGTCAA GTCGGGTGGC 
ACCTATTTCC TTATTTCCCG GAGTCTGGGA CCAGAGCTGG GAGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCTGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAAACCGTA 
AGGGACCTAC TGCAGGAGTA TGGCACACCC ATCGTAGACC CCATCAATGA CATCCGCATC 
ATCGGTGTGG TCACGGTCAC TGTGCTGCTG GCCATCTCGC TGGCTGGGAT GGAGTGGGAG 
TCCAAGGCCC AGGTGCTATT CTTTCTTGTC ATCATGGTCT CCTTTGCCAA CTACCTGGTG 
GGCACACTGA TCCCAGCATC TGAAGACAAA GCCTCTAAAG GCTTCTACAG CTACCATGGG 
GACATTTTTG TTCAGAACCT GGTACCCGAC TGGAGAGGCA TTGATGGTAG CTTCTTTGGC 
ATGTTCTCCA TCTTCTTCCC TTCGGCCACT GGCATTCTGG CAGGGGCCAA CATCTCCGGG 
GACCTCAAGG ATCCTGCTGT AGCCATCCCC AAGGGAACAC TCATGGCCAT TTTCTGGACC 
ACCATCTCCT ACCTTGCCAT CTCAGCTACC ATCGGCTCCT GTGTGGTGAG AGATGCCTCT 
GGGGATGTGA ACGACACCAT GACTCCTGGT CCAGGCCCCT GCGAAGGGCT AGCCTGTGGC 
TATGGCTGGA ACTTCACAGA ATGCTCCCAG CAGCGCAGTT GCCGCTATGG CCTCATCAAC 
TACTACCAGA CCATGAGCAT GGTGTCTGCC TTCGCACCCC TGATCACAGC CGGCATCTTT 
GGCGCCACCC TGTCTTCTGC CTTGGCCTGT CTTGTCTCGG CTGCTAAGGT CTTCCAGTGC 
CTGTGTGAAG ACCAGCTGTA CCCTCTGATT GGCTTTTTTG GCAAAGGCTA TGGCAAGAAC 
AGGGAGCCTG TGCGTGGCTA CCTGCTGGCC TATGCCATTG CTGTGGCCTT CATCATCATT 
GCTGAACTTA ACACCATCGC CCCCATCATT TCCAACTTCT TCCTCTGCTC CTACGCCCTC 
ATCAATTTCA GCTGCTTCCA TGCCTCCATC ACCAACTCAC CTGGATGGAG ACCCTCCTTC 
CGATACTACA GCAAGTGGGC GGCGCTGTTT GGGGCAGTGA TCTCGGTGGT CATCATGTTC 
CTGCTTACCT GGTGGGCTGC TCTCATTGCC ATCGGAGTCG TCCTCTTCCT CCTGCTCTAT 
GTGATCTACA AGAAGCCAGA GGTGAACTGG GGTTCCTCAG TACAGGCTGG CTCCTACAAT 
CTGGCCCTGA GCTACTCCGT GGGCCTCAAC GAGGTGGAAG ACCACATCAA GAACTACCGC 
CCCCAGTGCC TGGTGCTTAC AGGGCCCCCC AACTTCCGCC CAGCCCTTGT GGACTTTGTG 
AGCACATTTA CCCAGAACCT AAGCCTAATG ATTTGTGGTC ATGTGCTCAT TGGACCTGGC 
AAGCAGAGAG TGCCCGAGCT CCGGCTCATC GCCAGTGGAC ACACCAAGTG GCTGAACAAG 
AGGAAGATCA AGGCCTTCTA CTCTGACGTC ATCGCTGAGG ACTTGCGCAG TGGCGTCCAG 
ATTCTCATGC AGGCCTCAGG GCTTGGGAGA ATGAAGCCGA ACATTCTGGT GGTTGGATTC 
AAGAGGAACT GGCAGTCTGC CCACCCAGCC ACAGTGGAGG ACTACATTGG TGTCCTGCAT 
GATGCCTTTG ACTTCAACTA CGGGGTTTGT GTCATGAGGA TGCGCGAGGG GCTCAATGTC 
TCCGAGGCTC TGCAGACCCA CACAACCCCA GAGGCATTGA TACAAGAGGA GCAGGCTAGC 
ACCATCTTCC AGTCGGAGCA AGGCAAGAAG ACCATTGACA TCTACTGGCT GTTCGATGAT 
GGAGGTCTCA CCCTCCTCAT CCCCTATCTC CTGCACCGCA AGAAGAGATG GGGCAAATGC 
AAGATCCGAG TGTTTGTTGG GGGCCAGATC AACAGGATGG ACGAAGAGAG AAAGGCGATC 
ATCTCTCTGT TGAGCAAGTT CAGACTGGGA TTCCATGAAG TTCACGTCCT TCCCGACATC 
AACCAGAAGC CCCAGGCCGA GCACACCAAG CGGTTTGAGG ACATGATTGC ACCCTTCCGC 
CTCAATGATG GCTTCAAGGA CGAGGCCACC GTCACTGAGA TGAGGAGGGA CTGTCCCTGG 
AAGATCTCAG ATGAAGAGAT TAACAAGAAC AGAATTAAGT CCCTTCGGCA GGTGAGACTG 
AGTGAGATAC TGCTGGATTA CTCTCGGGAT GCTGCTCTGA TCATCCTTAC CCTGCCCATT 
GGAAGGAAGG GGAAGTGCCC CAGTTCGCTG TACATGGCCT GGCTGGAGAC CCTGTCCCAG 
GACCTCAGAC CTCCCGTCCT CCTCATCCGA GGCAACCAGG AGAATGTGCT CACCTTTTAC 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192240.1
CDS	31..3039
Translation	MAELPVTELPGDALCSGRFTISTLMGGDEPPPAACDSSQPSHLTHGSTLYMRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRQGRELTDGLVEDETGTNSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASEDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTMTPGPGPCEGLACGYGWNFTECSQQRSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNREPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIGPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKRNWQSAHPATVEDYIGVLHDAFDFNYGVCVMRMREGLNVSEALQTHTTPEALIQEEQASTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQAEHTKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLSEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLRPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_001205311.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205311.1

ORF Insert Sequence:

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCA AGGTCGTGAG CTGACTGATG GACTTGTGGA AGATGAGACA 
GGCACCAACA GTGAGAAGAG CCCTGGAGAA CCTGTTCGCT TCGGCTGGGT CAAGGGGGTG 
ATGATCCGTT GCATGCTCAA CATCTGGGGA GTGATCCTCT ACCTGCGACT CCCCTGGATT 
ACGGCGCAGG CGGGCATCGT GCTGACTTGG CTCATCATCC TGCTGTCGGT CATGGTGACA 
TCCATCACCG GCCTCTCCAT CTCCGCCATC TCTACCAATG GCAAGGTCAA GTCGGGTGGC 
ACCTATTTCC TTATTTCCCG GAGTCTGGGA CCAGAGCTGG GAGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCTGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAAACCGTA 
AGGGACCTAC TGCAGGAGTA TGGCACACCC ATCGTAGACC CCATCAATGA CATCCGCATC 
ATCGGTGTGG TCACGGTCAC TGTGCTGCTG GCCATCTCGC TGGCTGGGAT GGAGTGGGAG 
TCCAAGGCCC AGGTGCTATT CTTTCTTGTC ATCATGGTCT CCTTTGCCAA CTACCTGGTG 
GGCACACTGA TCCCAGCATC TGAAGACAAA GCCTCTAAAG GCTTCTACAG CTACCATGGG 
GACATTTTTG TTCAGAACCT GGTACCCGAC TGGAGAGGCA TTGATGGTAG CTTCTTTGGC 
ATGTTCTCCA TCTTCTTCCC TTCGGCCACT GGCATTCTGG CAGGGGCCAA CATCTCCGGG 
GACCTCAAGG ATCCTGCTGT AGCCATCCCC AAGGGAACAC TCATGGCCAT TTTCTGGACC 
ACCATCTCCT ACCTTGCCAT CTCAGCTACC ATCGGCTCCT GTGTGGTGAG AGATGCCTCT 
GGGGATGTGA ACGACACCAT GACTCCTGGT CCAGGCCCCT GCGAAGGGCT AGCCTGTGGC 
TATGGCTGGA ACTTCACAGA ATGCTCCCAG CAGCGCAGTT GCCGCTATGG CCTCATCAAC 
TACTACCAGA CCATGAGCAT GGTGTCTGCC TTCGCACCCC TGATCACAGC CGGCATCTTT 
GGCGCCACCC TGTCTTCTGC CTTGGCCTGT CTTGTCTCGG CTGCTAAGGT CTTCCAGTGC 
CTGTGTGAAG ACCAGCTGTA CCCTCTGATT GGCTTTTTTG GCAAAGGCTA TGGCAAGAAC 
AGGGAGCCTG TGCGTGGCTA CCTGCTGGCC TATGCCATTG CTGTGGCCTT CATCATCATT 
GCTGAACTTA ACACCATCGC CCCCATCATT TCCAACTTCT TCCTCTGCTC CTACGCCCTC 
ATCAATTTCA GCTGCTTCCA TGCCTCCATC ACCAACTCAC CTGGATGGAG ACCCTCCTTC 
CGATACTACA GCAAGTGGGC GGCGCTGTTT GGGGCAGTGA TCTCGGTGGT CATCATGTTC 
CTGCTTACCT GGTGGGCTGC TCTCATTGCC ATCGGAGTCG TCCTCTTCCT CCTGCTCTAT 
GTGATCTACA AGAAGCCAGA GGTGAACTGG GGTTCCTCAG TACAGGCTGG CTCCTACAAT 
CTGGCCCTGA GCTACTCCGT GGGCCTCAAC GAGGTGGAAG ACCACATCAA GAACTACCGC 
CCCCAGTGCC TGGTGCTTAC AGGGCCCCCC AACTTCCGCC CAGCCCTTGT GGACTTTGTG 
AGCACATTTA CCCAGAACCT AAGCCTAATG ATTTGTGGTC ATGTGCTCAT TGGACCTGGC 
AAGCAGAGAG TGCCCGAGCT CCGGCTCATC GCCAGTGGAC ACACCAAGTG GCTGAACAAG 
AGGAAGATCA AGGCCTTCTA CTCTGACGTC ATCGCTGAGG ACTTGCGCAG TGGCGTCCAG 
ATTCTCATGC AGGCCTCAGG GCTTGGGAGA ATGAAGCCGA ACATTCTGGT GGTTGGATTC 
AAGAGGAACT GGCAGTCTGC CCACCCAGCC ACAGTGGAGG ACTACATTGG TGTCCTGCAT 
GATGCCTTTG ACTTCAACTA CGGGGTTTGT GTCATGAGGA TGCGCGAGGG GCTCAATGTC 
TCCGAGGCTC TGCAGACCCA CACAACCCCA GAGGCATTGA TACAAGAGGA GCAGGCTAGC 
ACCATCTTCC AGTCGGAGCA AGGCAAGAAG ACCATTGACA TCTACTGGCT GTTCGATGAT 
GGAGGTCTCA CCCTCCTCAT CCCCTATCTC CTGCACCGCA AGAAGAGATG GGGCAAATGC 
AAGATCCGAG TGTTTGTTGG GGGCCAGATC AACAGGATGG ACGAAGAGAG AAAGGCGATC 
ATCTCTCTGT TGAGCAAGTT CAGACTGGGA TTCCATGAAG TTCACGTCCT TCCCGACATC 
AACCAGAAGC CCCAGGCCGA GCACACCAAG CGGTTTGAGG ACATGATTGC ACCCTTCCGC 
CTCAATGATG GCTTCAAGGA CGAGGCCACC GTCACTGAGA TGAGGAGGGA CTGTCCCTGG 
AAGATCTCAG ATGAAGAGAT TAACAAGAAC AGAATTAAGT CCCTTCGGCA GGTGAGACTG 
AGTGAGATAC TGCTGGATTA CTCTCGGGAT GCTGCTCTGA TCATCCTTAC CCTGCCCATT 
GGAAGGAAGG GGAAGTGCCC CAGTTCGCTG TACATGGCCT GGCTGGAGAC CCTGTCCCAG 
GACCTCAGAC CTCCCGTCCT CCTCATCCGA GGCAACCAGG AGAATGTGCT CACCTTTTAC 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( XM_017312625.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168114.1 Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82384
Clone ID Related Accession (Same CDS sequence)	XM_017312625.1
Accession Version	XM_017312625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCA AGGTCGTGAG CTGACTGATG GACTTGTGGA AGATGAGACA 
GGCACCAACA GTGAGAAGAG CCCTGGAGAA CCTGTTCGCT TCGGCTGGGT CAAGGGGGTG 
ATGATCCGTT GCATGCTCAA CATCTGGGGA GTGATCCTCT ACCTGCGACT CCCCTGGATT 
ACGGCGCAGG CGGGCATCGT GCTGACTTGG CTCATCATCC TGCTGTCGGT CATGGTGACA 
TCCATCACCG GCCTCTCCAT CTCCGCCATC TCTACCAATG GCAAGGTCAA GTCGGGTGGC 
ACCTATTTCC TTATTTCCCG GAGTCTGGGA CCAGAGCTGG GAGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCTGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAAACCGTA 
AGGGACCTAC TGCAGCTGGC CTGCTCCCCC ACTCAGGAGT ATGGCACACC CATCGTAGAC 
CCCATCAATG ACATCCGCAT CATCGGTGTG GTCACGGTCA CTGTGCTGCT GGCCATCTCG 
CTGGCTGGGA TGGAGTGGGA GTCCAAGGCC CAGGTGCTAT TCTTTCTTGT CATCATGGTC 
TCCTTTGCCA ACTACCTGGT GGGCACACTG ATCCCAGCAT CTGAAGACAA AGCCTCTAAA 
GGCTTCTACA GCTACCATGG GGACATTTTT GTTCAGAACC TGGTACCCGA CTGGAGAGGC 
ATTGATGGTA GCTTCTTTGG CATGTTCTCC ATCTTCTTCC CTTCGGCCAC TGGCATTCTG 
GCAGGGGCCA ACATCTCCGG GGACCTCAAG GATCCTGCTG TAGCCATCCC CAAGGGAACA 
CTCATGGCCA TTTTCTGGAC CACCATCTCC TACCTTGCCA TCTCAGCTAC CATCGGCTCC 
TGTGTGGTGA GAGATGCCTC TGGGGATGTG AACGACACCA TGACTCCTGG TCCAGGCCCC 
TGCGAAGGGC TAGCCTGTGG CTATGGCTGG AACTTCACAG AATGCTCCCA GCAGCGCAGT 
TGCCGCTATG GCCTCATCAA CTACTACCAG ACCATGAGCA TGGTGTCTGC CTTCGCACCC 
CTGATCACAG CCGGCATCTT TGGCGCCACC CTGTCTTCTG CCTTGGCCTG TCTTGTCTCG 
GCTGCTAAGG TCTTCCAGTG CCTGTGTGAA GACCAGCTGT ACCCTCTGAT TGGCTTTTTT 
GGCAAAGGCT ATGGCAAGAA CAGGGAGCCT GTGCGTGGCT ACCTGCTGGC CTATGCCATT 
GCTGTGGCCT TCATCATCAT TGCTGAACTT AACACCATCG CCCCCATCAT TTCCAACTTC 
TTCCTCTGCT CCTACGCCCT CATCAATTTC AGCTGCTTCC ATGCCTCCAT CACCAACTCA 
CCTGGATGGA GACCCTCCTT CCGATACTAC AGCAAGTGGG CGGCGCTGTT TGGGGCAGTG 
ATCTCGGTGG TCATCATGTT CCTGCTTACC TGGTGGGCTG CTCTCATTGC CATCGGAGTC 
GTCCTCTTCC TCCTGCTCTA TGTGATCTAC AAGAAGCCAG AGGTGAACTG GGGTTCCTCA 
GTACAGGCTG GCTCCTACAA TCTGGCCCTG AGCTACTCCG TGGGCCTCAA CGAGGTGGAA 
GACCACATCA AGAACTACCG CCCCCAGTGC CTGGTGCTTA CAGGGCCCCC CAACTTCCGC 
CCAGCCCTTG TGGACTTTGT GAGCACATTT ACCCAGAACC TAAGCCTAAT GATTTGTGGT 
CATGTGCTCA TTGGACCTGG CAAGCAGAGA GTGCCCGAGC TCCGGCTCAT CGCCAGTGGA 
CACACCAAGT GGCTGAACAA GAGGAAGATC AAGGCCTTCT ACTCTGACGT CATCGCTGAG 
GACTTGCGCA GTGGCGTCCA GATTCTCATG CAGGCCTCAG GGCTTGGGAG AATGAAGCCG 
AACATTCTGG TGGTTGGATT CAAGAGGAAC TGGCAGTCTG CCCACCCAGC CACAGTGGAG 
GACTACATTG GTGTCCTGCA TGATGCCTTT GACTTCAACT ACGGGGTTTG TGTCATGAGG 
ATGCGCGAGG GGCTCAATGT CTCCGAGGCT CTGCAGACCC ACACAACCCC AGAGGCATTG 
ATACAAGAGG AGCAGGCTAG CACCATCTTC CAGTCGGAGC AAGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTCGATGA TGGAGGTCTC ACCCTCCTCA TCCCCTATCT CCTGCACCGC 
AAGAAGAGAT GGGGCAAATG CAAGATCCGA GTGTTTGTTG GGGGCCAGAT CAACAGGATG 
GACGAAGAGA GAAAGGCGAT CATCTCTCTG TTGAGCAAGT TCAGACTGGG ATTCCATGAA 
GTTCACGTCC TTCCCGACAT CAACCAGAAG CCCCAGGCCG AGCACACCAA GCGGTTTGAG 
GACATGATTG CACCCTTCCG CCTCAATGAT GGCTTCAAGG ACGAGGCCAC CGTCACTGAG 
ATGAGGAGGG ACTGTCCCTG GAAGATCTCA GATGAAGAGA TTAACAAGAA CAGAATTAAG 
TCCCTTCGGC AGGTGAGACT GAGTGAGATA CTGCTGGATT ACTCTCGGGA TGCTGCTCTG 
ATCATCCTTA CCCTGCCCAT TGGAAGGAAG GGGAAGTGCC CCAGTTCGCT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGA CCTCCCGTCC TCCTCATCCG AGGCAACCAG 
GAGAATGTGC TCACCTTTTA CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168114.1
CDS	128..3157
Translation	MAELPVTELPGDALCSGRFTISTLMGGDEPPPAACDSSQPSHLTHGSTLYMRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRQGRELTDGLVEDETGTNSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQLACSPTQEYGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASEDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTMTPGPGPCEGLACGYGWNFTECSQQRSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNREPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIGPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKRNWQSAHPATVEDYIGVLHDAFDFNYGVCVMRMREGLNVSEALQTHTTPEALIQEEQASTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQAEHTKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLSEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLRPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_017312625.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312625.1

ORF Insert Sequence:

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCA AGGTCGTGAG CTGACTGATG GACTTGTGGA AGATGAGACA 
GGCACCAACA GTGAGAAGAG CCCTGGAGAA CCTGTTCGCT TCGGCTGGGT CAAGGGGGTG 
ATGATCCGTT GCATGCTCAA CATCTGGGGA GTGATCCTCT ACCTGCGACT CCCCTGGATT 
ACGGCGCAGG CGGGCATCGT GCTGACTTGG CTCATCATCC TGCTGTCGGT CATGGTGACA 
TCCATCACCG GCCTCTCCAT CTCCGCCATC TCTACCAATG GCAAGGTCAA GTCGGGTGGC 
ACCTATTTCC TTATTTCCCG GAGTCTGGGA CCAGAGCTGG GAGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCTGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAAACCGTA 
AGGGACCTAC TGCAGCTGGC CTGCTCCCCC ACTCAGGAGT ATGGCACACC CATCGTAGAC 
CCCATCAATG ACATCCGCAT CATCGGTGTG GTCACGGTCA CTGTGCTGCT GGCCATCTCG 
CTGGCTGGGA TGGAGTGGGA GTCCAAGGCC CAGGTGCTAT TCTTTCTTGT CATCATGGTC 
TCCTTTGCCA ACTACCTGGT GGGCACACTG ATCCCAGCAT CTGAAGACAA AGCCTCTAAA 
GGCTTCTACA GCTACCATGG GGACATTTTT GTTCAGAACC TGGTACCCGA CTGGAGAGGC 
ATTGATGGTA GCTTCTTTGG CATGTTCTCC ATCTTCTTCC CTTCGGCCAC TGGCATTCTG 
GCAGGGGCCA ACATCTCCGG GGACCTCAAG GATCCTGCTG TAGCCATCCC CAAGGGAACA 
CTCATGGCCA TTTTCTGGAC CACCATCTCC TACCTTGCCA TCTCAGCTAC CATCGGCTCC 
TGTGTGGTGA GAGATGCCTC TGGGGATGTG AACGACACCA TGACTCCTGG TCCAGGCCCC 
TGCGAAGGGC TAGCCTGTGG CTATGGCTGG AACTTCACAG AATGCTCCCA GCAGCGCAGT 
TGCCGCTATG GCCTCATCAA CTACTACCAG ACCATGAGCA TGGTGTCTGC CTTCGCACCC 
CTGATCACAG CCGGCATCTT TGGCGCCACC CTGTCTTCTG CCTTGGCCTG TCTTGTCTCG 
GCTGCTAAGG TCTTCCAGTG CCTGTGTGAA GACCAGCTGT ACCCTCTGAT TGGCTTTTTT 
GGCAAAGGCT ATGGCAAGAA CAGGGAGCCT GTGCGTGGCT ACCTGCTGGC CTATGCCATT 
GCTGTGGCCT TCATCATCAT TGCTGAACTT AACACCATCG CCCCCATCAT TTCCAACTTC 
TTCCTCTGCT CCTACGCCCT CATCAATTTC AGCTGCTTCC ATGCCTCCAT CACCAACTCA 
CCTGGATGGA GACCCTCCTT CCGATACTAC AGCAAGTGGG CGGCGCTGTT TGGGGCAGTG 
ATCTCGGTGG TCATCATGTT CCTGCTTACC TGGTGGGCTG CTCTCATTGC CATCGGAGTC 
GTCCTCTTCC TCCTGCTCTA TGTGATCTAC AAGAAGCCAG AGGTGAACTG GGGTTCCTCA 
GTACAGGCTG GCTCCTACAA TCTGGCCCTG AGCTACTCCG TGGGCCTCAA CGAGGTGGAA 
GACCACATCA AGAACTACCG CCCCCAGTGC CTGGTGCTTA CAGGGCCCCC CAACTTCCGC 
CCAGCCCTTG TGGACTTTGT GAGCACATTT ACCCAGAACC TAAGCCTAAT GATTTGTGGT 
CATGTGCTCA TTGGACCTGG CAAGCAGAGA GTGCCCGAGC TCCGGCTCAT CGCCAGTGGA 
CACACCAAGT GGCTGAACAA GAGGAAGATC AAGGCCTTCT ACTCTGACGT CATCGCTGAG 
GACTTGCGCA GTGGCGTCCA GATTCTCATG CAGGCCTCAG GGCTTGGGAG AATGAAGCCG 
AACATTCTGG TGGTTGGATT CAAGAGGAAC TGGCAGTCTG CCCACCCAGC CACAGTGGAG 
GACTACATTG GTGTCCTGCA TGATGCCTTT GACTTCAACT ACGGGGTTTG TGTCATGAGG 
ATGCGCGAGG GGCTCAATGT CTCCGAGGCT CTGCAGACCC ACACAACCCC AGAGGCATTG 
ATACAAGAGG AGCAGGCTAG CACCATCTTC CAGTCGGAGC AAGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTCGATGA TGGAGGTCTC ACCCTCCTCA TCCCCTATCT CCTGCACCGC 
AAGAAGAGAT GGGGCAAATG CAAGATCCGA GTGTTTGTTG GGGGCCAGAT CAACAGGATG 
GACGAAGAGA GAAAGGCGAT CATCTCTCTG TTGAGCAAGT TCAGACTGGG ATTCCATGAA 
GTTCACGTCC TTCCCGACAT CAACCAGAAG CCCCAGGCCG AGCACACCAA GCGGTTTGAG 
GACATGATTG CACCCTTCCG CCTCAATGAT GGCTTCAAGG ACGAGGCCAC CGTCACTGAG 
ATGAGGAGGG ACTGTCCCTG GAAGATCTCA GATGAAGAGA TTAACAAGAA CAGAATTAAG 
TCCCTTCGGC AGGTGAGACT GAGTGAGATA CTGCTGGATT ACTCTCGGGA TGCTGCTCTG 
ATCATCCTTA CCCTGCCCAT TGGAAGGAAG GGGAAGTGCC CCAGTTCGCT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGA CCTCCCGTCC TCCTCATCCG AGGCAACCAG 
GAGAATGTGC TCACCTTTTA CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( XM_017312626.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168115.1 Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu82385
Clone ID Related Accession (Same CDS sequence)	XM_017312626.1
Accession Version	XM_017312626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCAAGG TCGTGAGCTG ACTGATGGAC TTGTGGAAGA TGAGACAGGC 
ACCAACAGTG AGAAGAGCCC TGGAGAACCT GTTCGCTTCG GCTGGGTCAA GGGGGTGATG 
ATCCGTTGCA TGCTCAACAT CTGGGGAGTG ATCCTCTACC TGCGACTCCC CTGGATTACG 
GCGCAGGCGG GCATCGTGCT GACTTGGCTC ATCATCCTGC TGTCGGTCAT GGTGACATCC 
ATCACCGGCC TCTCCATCTC CGCCATCTCT ACCAATGGCA AGGTCAAGTC GGGTGGCACC 
TATTTCCTTA TTTCCCGGAG TCTGGGACCA GAGCTGGGAG GCTCCATTGG CCTCATCTTC 
GCCTTTGCCA ATGCTGTGGG TGTGGCCATG CACACTGTGG GCTTTGCAGA AACCGTAAGG 
GACCTACTGC AGCTGGCCTG CTCCCCCACT CAGGAGTATG GCACACCCAT CGTAGACCCC 
ATCAATGACA TCCGCATCAT CGGTGTGGTC ACGGTCACTG TGCTGCTGGC CATCTCGCTG 
GCTGGGATGG AGTGGGAGTC CAAGGCCCAG GTGCTATTCT TTCTTGTCAT CATGGTCTCC 
TTTGCCAACT ACCTGGTGGG CACACTGATC CCAGCATCTG AAGACAAAGC CTCTAAAGGC 
TTCTACAGCT ACCATGGGGA CATTTTTGTT CAGAACCTGG TACCCGACTG GAGAGGCATT 
GATGGTAGCT TCTTTGGCAT GTTCTCCATC TTCTTCCCTT CGGCCACTGG CATTCTGGCA 
GGGGCCAACA TCTCCGGGGA CCTCAAGGAT CCTGCTGTAG CCATCCCCAA GGGAACACTC 
ATGGCCATTT TCTGGACCAC CATCTCCTAC CTTGCCATCT CAGCTACCAT CGGCTCCTGT 
GTGGTGAGAG ATGCCTCTGG GGATGTGAAC GACACCATGA CTCCTGGTCC AGGCCCCTGC 
GAAGGGCTAG CCTGTGGCTA TGGCTGGAAC TTCACAGAAT GCTCCCAGCA GCGCAGTTGC 
CGCTATGGCC TCATCAACTA CTACCAGACC ATGAGCATGG TGTCTGCCTT CGCACCCCTG 
ATCACAGCCG GCATCTTTGG CGCCACCCTG TCTTCTGCCT TGGCCTGTCT TGTCTCGGCT 
GCTAAGGTCT TCCAGTGCCT GTGTGAAGAC CAGCTGTACC CTCTGATTGG CTTTTTTGGC 
AAAGGCTATG GCAAGAACAG GGAGCCTGTG CGTGGCTACC TGCTGGCCTA TGCCATTGCT 
GTGGCCTTCA TCATCATTGC TGAACTTAAC ACCATCGCCC CCATCATTTC CAACTTCTTC 
CTCTGCTCCT ACGCCCTCAT CAATTTCAGC TGCTTCCATG CCTCCATCAC CAACTCACCT 
GGATGGAGAC CCTCCTTCCG ATACTACAGC AAGTGGGCGG CGCTGTTTGG GGCAGTGATC 
TCGGTGGTCA TCATGTTCCT GCTTACCTGG TGGGCTGCTC TCATTGCCAT CGGAGTCGTC 
CTCTTCCTCC TGCTCTATGT GATCTACAAG AAGCCAGAGG TGAACTGGGG TTCCTCAGTA 
CAGGCTGGCT CCTACAATCT GGCCCTGAGC TACTCCGTGG GCCTCAACGA GGTGGAAGAC 
CACATCAAGA ACTACCGCCC CCAGTGCCTG GTGCTTACAG GGCCCCCCAA CTTCCGCCCA 
GCCCTTGTGG ACTTTGTGAG CACATTTACC CAGAACCTAA GCCTAATGAT TTGTGGTCAT 
GTGCTCATTG GACCTGGCAA GCAGAGAGTG CCCGAGCTCC GGCTCATCGC CAGTGGACAC 
ACCAAGTGGC TGAACAAGAG GAAGATCAAG GCCTTCTACT CTGACGTCAT CGCTGAGGAC 
TTGCGCAGTG GCGTCCAGAT TCTCATGCAG GCCTCAGGGC TTGGGAGAAT GAAGCCGAAC 
ATTCTGGTGG TTGGATTCAA GAGGAACTGG CAGTCTGCCC ACCCAGCCAC AGTGGAGGAC 
TACATTGGTG TCCTGCATGA TGCCTTTGAC TTCAACTACG GGGTTTGTGT CATGAGGATG 
CGCGAGGGGC TCAATGTCTC CGAGGCTCTG CAGACCCACA CAACCCCAGA GGCATTGATA 
CAAGAGGAGC AGGCTAGCAC CATCTTCCAG TCGGAGCAAG GCAAGAAGAC CATTGACATC 
TACTGGCTGT TCGATGATGG AGGTCTCACC CTCCTCATCC CCTATCTCCT GCACCGCAAG 
AAGAGATGGG GCAAATGCAA GATCCGAGTG TTTGTTGGGG GCCAGATCAA CAGGATGGAC 
GAAGAGAGAA AGGCGATCAT CTCTCTGTTG AGCAAGTTCA GACTGGGATT CCATGAAGTT 
CACGTCCTTC CCGACATCAA CCAGAAGCCC CAGGCCGAGC ACACCAAGCG GTTTGAGGAC 
ATGATTGCAC CCTTCCGCCT CAATGATGGC TTCAAGGACG AGGCCACCGT CACTGAGATG 
AGGAGGGACT GTCCCTGGAA GATCTCAGAT GAAGAGATTA ACAAGAACAG AATTAAGTCC 
CTTCGGCAGG TGAGACTGAG TGAGATACTG CTGGATTACT CTCGGGATGC TGCTCTGATC 
ATCCTTACCC TGCCCATTGG AAGGAAGGGG AAGTGCCCCA GTTCGCTGTA CATGGCCTGG 
CTGGAGACCC TGTCCCAGGA CCTCAGACCT CCCGTCCTCC TCATCCGAGG CAACCAGGAG 
AATGTGCTCA CCTTTTACTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168115.1
CDS	128..3154
Translation	MAELPVTELPGDALCSGRFTISTLMGGDEPPPAACDSSQPSHLTHGSTLYMRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKEGSHLHALAFDGRQGRELTDGLVEDETGTNSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQLACSPTQEYGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASEDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTMTPGPGPCEGLACGYGWNFTECSQQRSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNREPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIGPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKRNWQSAHPATVEDYIGVLHDAFDFNYGVCVMRMREGLNVSEALQTHTTPEALIQEEQASTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQAEHTKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLSEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLRPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_017312626.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312626.1

ORF Insert Sequence:

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCAAGG TCGTGAGCTG ACTGATGGAC TTGTGGAAGA TGAGACAGGC 
ACCAACAGTG AGAAGAGCCC TGGAGAACCT GTTCGCTTCG GCTGGGTCAA GGGGGTGATG 
ATCCGTTGCA TGCTCAACAT CTGGGGAGTG ATCCTCTACC TGCGACTCCC CTGGATTACG 
GCGCAGGCGG GCATCGTGCT GACTTGGCTC ATCATCCTGC TGTCGGTCAT GGTGACATCC 
ATCACCGGCC TCTCCATCTC CGCCATCTCT ACCAATGGCA AGGTCAAGTC GGGTGGCACC 
TATTTCCTTA TTTCCCGGAG TCTGGGACCA GAGCTGGGAG GCTCCATTGG CCTCATCTTC 
GCCTTTGCCA ATGCTGTGGG TGTGGCCATG CACACTGTGG GCTTTGCAGA AACCGTAAGG 
GACCTACTGC AGCTGGCCTG CTCCCCCACT CAGGAGTATG GCACACCCAT CGTAGACCCC 
ATCAATGACA TCCGCATCAT CGGTGTGGTC ACGGTCACTG TGCTGCTGGC CATCTCGCTG 
GCTGGGATGG AGTGGGAGTC CAAGGCCCAG GTGCTATTCT TTCTTGTCAT CATGGTCTCC 
TTTGCCAACT ACCTGGTGGG CACACTGATC CCAGCATCTG AAGACAAAGC CTCTAAAGGC 
TTCTACAGCT ACCATGGGGA CATTTTTGTT CAGAACCTGG TACCCGACTG GAGAGGCATT 
GATGGTAGCT TCTTTGGCAT GTTCTCCATC TTCTTCCCTT CGGCCACTGG CATTCTGGCA 
GGGGCCAACA TCTCCGGGGA CCTCAAGGAT CCTGCTGTAG CCATCCCCAA GGGAACACTC 
ATGGCCATTT TCTGGACCAC CATCTCCTAC CTTGCCATCT CAGCTACCAT CGGCTCCTGT 
GTGGTGAGAG ATGCCTCTGG GGATGTGAAC GACACCATGA CTCCTGGTCC AGGCCCCTGC 
GAAGGGCTAG CCTGTGGCTA TGGCTGGAAC TTCACAGAAT GCTCCCAGCA GCGCAGTTGC 
CGCTATGGCC TCATCAACTA CTACCAGACC ATGAGCATGG TGTCTGCCTT CGCACCCCTG 
ATCACAGCCG GCATCTTTGG CGCCACCCTG TCTTCTGCCT TGGCCTGTCT TGTCTCGGCT 
GCTAAGGTCT TCCAGTGCCT GTGTGAAGAC CAGCTGTACC CTCTGATTGG CTTTTTTGGC 
AAAGGCTATG GCAAGAACAG GGAGCCTGTG CGTGGCTACC TGCTGGCCTA TGCCATTGCT 
GTGGCCTTCA TCATCATTGC TGAACTTAAC ACCATCGCCC CCATCATTTC CAACTTCTTC 
CTCTGCTCCT ACGCCCTCAT CAATTTCAGC TGCTTCCATG CCTCCATCAC CAACTCACCT 
GGATGGAGAC CCTCCTTCCG ATACTACAGC AAGTGGGCGG CGCTGTTTGG GGCAGTGATC 
TCGGTGGTCA TCATGTTCCT GCTTACCTGG TGGGCTGCTC TCATTGCCAT CGGAGTCGTC 
CTCTTCCTCC TGCTCTATGT GATCTACAAG AAGCCAGAGG TGAACTGGGG TTCCTCAGTA 
CAGGCTGGCT CCTACAATCT GGCCCTGAGC TACTCCGTGG GCCTCAACGA GGTGGAAGAC 
CACATCAAGA ACTACCGCCC CCAGTGCCTG GTGCTTACAG GGCCCCCCAA CTTCCGCCCA 
GCCCTTGTGG ACTTTGTGAG CACATTTACC CAGAACCTAA GCCTAATGAT TTGTGGTCAT 
GTGCTCATTG GACCTGGCAA GCAGAGAGTG CCCGAGCTCC GGCTCATCGC CAGTGGACAC 
ACCAAGTGGC TGAACAAGAG GAAGATCAAG GCCTTCTACT CTGACGTCAT CGCTGAGGAC 
TTGCGCAGTG GCGTCCAGAT TCTCATGCAG GCCTCAGGGC TTGGGAGAAT GAAGCCGAAC 
ATTCTGGTGG TTGGATTCAA GAGGAACTGG CAGTCTGCCC ACCCAGCCAC AGTGGAGGAC 
TACATTGGTG TCCTGCATGA TGCCTTTGAC TTCAACTACG GGGTTTGTGT CATGAGGATG 
CGCGAGGGGC TCAATGTCTC CGAGGCTCTG CAGACCCACA CAACCCCAGA GGCATTGATA 
CAAGAGGAGC AGGCTAGCAC CATCTTCCAG TCGGAGCAAG GCAAGAAGAC CATTGACATC 
TACTGGCTGT TCGATGATGG AGGTCTCACC CTCCTCATCC CCTATCTCCT GCACCGCAAG 
AAGAGATGGG GCAAATGCAA GATCCGAGTG TTTGTTGGGG GCCAGATCAA CAGGATGGAC 
GAAGAGAGAA AGGCGATCAT CTCTCTGTTG AGCAAGTTCA GACTGGGATT CCATGAAGTT 
CACGTCCTTC CCGACATCAA CCAGAAGCCC CAGGCCGAGC ACACCAAGCG GTTTGAGGAC 
ATGATTGCAC CCTTCCGCCT CAATGATGGC TTCAAGGACG AGGCCACCGT CACTGAGATG 
AGGAGGGACT GTCCCTGGAA GATCTCAGAT GAAGAGATTA ACAAGAACAG AATTAAGTCC 
CTTCGGCAGG TGAGACTGAG TGAGATACTG CTGGATTACT CTCGGGATGC TGCTCTGATC 
ATCCTTACCC TGCCCATTGG AAGGAAGGGG AAGTGCCCCA GTTCGCTGTA CATGGCCTGG 
CTGGAGACCC TGTCCCAGGA CCTCAGACCT CCCGTCCTCC TCATCCGAGG CAACCAGGAG 
AATGTGCTCA CCTTTTACTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( NM_019415.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062288.2 Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant 2, mRNA.

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CloneID	OMu20583
Clone ID Related Accession (Same CDS sequence)	NM_019415.2
Accession Version	NM_019415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052691.1, AI882166.1 and AI647800.1. On or before Jul 26, 2011 this sequence version replaced XM_001472289.2, NM_019415.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U61085.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCAAGG TCGTGAGCTG ACTGATGGAC TTGTGGAAGA TGAGACAGGC 
ACCAACAGTG AGAAGAGCCC TGGAGAACCT GTTCGCTTCG GCTGGGTCAA GGGGGTGATG 
ATCCGTTGCA TGCTCAACAT CTGGGGAGTG ATCCTCTACC TGCGACTCCC CTGGATTACG 
GCGCAGGCGG GCATCGTGCT GACTTGGCTC ATCATCCTGC TGTCGGTCAT GGTGACATCC 
ATCACCGGCC TCTCCATCTC CGCCATCTCT ACCAATGGCA AGGTCAAGTC GGGTGGCACC 
TATTTCCTTA TTTCCCGGAG TCTGGGACCA GAGCTGGGAG GCTCCATTGG CCTCATCTTC 
GCCTTTGCCA ATGCTGTGGG TGTGGCCATG CACACTGTGG GCTTTGCAGA AACCGTAAGG 
GACCTACTGC AGGAGTATGG CACACCCATC GTAGACCCCA TCAATGACAT CCGCATCATC 
GGTGTGGTCA CGGTCACTGT GCTGCTGGCC ATCTCGCTGG CTGGGATGGA GTGGGAGTCC 
AAGGCCCAGG TGCTATTCTT TCTTGTCATC ATGGTCTCCT TTGCCAACTA CCTGGTGGGC 
ACACTGATCC CAGCATCTGA AGACAAAGCC TCTAAAGGCT TCTACAGCTA CCATGGGGAC 
ATTTTTGTTC AGAACCTGGT ACCCGACTGG AGAGGCATTG ATGGTAGCTT CTTTGGCATG 
TTCTCCATCT TCTTCCCTTC GGCCACTGGC ATTCTGGCAG GGGCCAACAT CTCCGGGGAC 
CTCAAGGATC CTGCTGTAGC CATCCCCAAG GGAACACTCA TGGCCATTTT CTGGACCACC 
ATCTCCTACC TTGCCATCTC AGCTACCATC GGCTCCTGTG TGGTGAGAGA TGCCTCTGGG 
GATGTGAACG ACACCATGAC TCCTGGTCCA GGCCCCTGCG AAGGGCTAGC CTGTGGCTAT 
GGCTGGAACT TCACAGAATG CTCCCAGCAG CGCAGTTGCC GCTATGGCCT CATCAACTAC 
TACCAGACCA TGAGCATGGT GTCTGCCTTC GCACCCCTGA TCACAGCCGG CATCTTTGGC 
GCCACCCTGT CTTCTGCCTT GGCCTGTCTT GTCTCGGCTG CTAAGGTCTT CCAGTGCCTG 
TGTGAAGACC AGCTGTACCC TCTGATTGGC TTTTTTGGCA AAGGCTATGG CAAGAACAGG 
GAGCCTGTGC GTGGCTACCT GCTGGCCTAT GCCATTGCTG TGGCCTTCAT CATCATTGCT 
GAACTTAACA CCATCGCCCC CATCATTTCC AACTTCTTCC TCTGCTCCTA CGCCCTCATC 
AATTTCAGCT GCTTCCATGC CTCCATCACC AACTCACCTG GATGGAGACC CTCCTTCCGA 
TACTACAGCA AGTGGGCGGC GCTGTTTGGG GCAGTGATCT CGGTGGTCAT CATGTTCCTG 
CTTACCTGGT GGGCTGCTCT CATTGCCATC GGAGTCGTCC TCTTCCTCCT GCTCTATGTG 
ATCTACAAGA AGCCAGAGGT GAACTGGGGT TCCTCAGTAC AGGCTGGCTC CTACAATCTG 
GCCCTGAGCT ACTCCGTGGG CCTCAACGAG GTGGAAGACC ACATCAAGAA CTACCGCCCC 
CAGTGCCTGG TGCTTACAGG GCCCCCCAAC TTCCGCCCAG CCCTTGTGGA CTTTGTGAGC 
ACATTTACCC AGAACCTAAG CCTAATGATT TGTGGTCATG TGCTCATTGG ACCTGGCAAG 
CAGAGAGTGC CCGAGCTCCG GCTCATCGCC AGTGGACACA CCAAGTGGCT GAACAAGAGG 
AAGATCAAGG CCTTCTACTC TGACGTCATC GCTGAGGACT TGCGCAGTGG CGTCCAGATT 
CTCATGCAGG CCTCAGGGCT TGGGAGAATG AAGCCGAACA TTCTGGTGGT TGGATTCAAG 
AGGAACTGGC AGTCTGCCCA CCCAGCCACA GTGGAGGACT ACATTGGTGT CCTGCATGAT 
GCCTTTGACT TCAACTACGG GGTTTGTGTC ATGAGGATGC GCGAGGGGCT CAATGTCTCC 
GAGGCTCTGC AGACCCACAC AACCCCAGAG GCATTGATAC AAGAGGAGCA GGCTAGCACC 
ATCTTCCAGT CGGAGCAAGG CAAGAAGACC ATTGACATCT ACTGGCTGTT CGATGATGGA 
GGTCTCACCC TCCTCATCCC CTATCTCCTG CACCGCAAGA AGAGATGGGG CAAATGCAAG 
ATCCGAGTGT TTGTTGGGGG CCAGATCAAC AGGATGGACG AAGAGAGAAA GGCGATCATC 
TCTCTGTTGA GCAAGTTCAG ACTGGGATTC CATGAAGTTC ACGTCCTTCC CGACATCAAC 
CAGAAGCCCC AGGCCGAGCA CACCAAGCGG TTTGAGGACA TGATTGCACC CTTCCGCCTC 
AATGATGGCT TCAAGGACGA GGCCACCGTC ACTGAGATGA GGAGGGACTG TCCCTGGAAG 
ATCTCAGATG AAGAGATTAA CAAGAACAGA ATTAAGTCCC TTCGGCAGGT GAGACTGAGT 
GAGATACTGC TGGATTACTC TCGGGATGCT GCTCTGATCA TCCTTACCCT GCCCATTGGA 
AGGAAGGGGA AGTGCCCCAG TTCGCTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC 
CTCAGACCTC CCGTCCTCCT CATCCGAGGC AACCAGGAGA ATGTGCTCAC CTTTTACTGC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062288.2
CDS	31..3036
Translation	MAELPVTELPGDALCSGRFTISTLMGGDEPPPAACDSSQPSHLTHGSTLYMRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKEGSHLHALAFDGRQGRELTDGLVEDETGTNSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASEDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTMTPGPGPCEGLACGYGWNFTECSQQRSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNREPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIGPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKRNWQSAHPATVEDYIGVLHDAFDFNYGVCVMRMREGLNVSEALQTHTTPEALIQEEQASTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQAEHTKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLSEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLRPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_019415.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 3 (Slc12a3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019415.2

ORF Insert Sequence:

ATGGCAGAGC TCCCCGTGAC AGAGCTGCCT GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACAC TGATGGGAGG CGATGAGCCC CCACCAGCTG CCTGTGACAG CAGCCAGCCC 
AGCCACTTAA CACACGGCAG CACCTTATAC ATGCGCACCT TCGGTTACAA CACCATAGAC 
GTGGTGCCGG CCTACGAACA CTATGCTAAC AGCGCTCTGC CCGGTGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCATTCATTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCAAGG TCGTGAGCTG ACTGATGGAC TTGTGGAAGA TGAGACAGGC 
ACCAACAGTG AGAAGAGCCC TGGAGAACCT GTTCGCTTCG GCTGGGTCAA GGGGGTGATG 
ATCCGTTGCA TGCTCAACAT CTGGGGAGTG ATCCTCTACC TGCGACTCCC CTGGATTACG 
GCGCAGGCGG GCATCGTGCT GACTTGGCTC ATCATCCTGC TGTCGGTCAT GGTGACATCC 
ATCACCGGCC TCTCCATCTC CGCCATCTCT ACCAATGGCA AGGTCAAGTC GGGTGGCACC 
TATTTCCTTA TTTCCCGGAG TCTGGGACCA GAGCTGGGAG GCTCCATTGG CCTCATCTTC 
GCCTTTGCCA ATGCTGTGGG TGTGGCCATG CACACTGTGG GCTTTGCAGA AACCGTAAGG 
GACCTACTGC AGGAGTATGG CACACCCATC GTAGACCCCA TCAATGACAT CCGCATCATC 
GGTGTGGTCA CGGTCACTGT GCTGCTGGCC ATCTCGCTGG CTGGGATGGA GTGGGAGTCC 
AAGGCCCAGG TGCTATTCTT TCTTGTCATC ATGGTCTCCT TTGCCAACTA CCTGGTGGGC 
ACACTGATCC CAGCATCTGA AGACAAAGCC TCTAAAGGCT TCTACAGCTA CCATGGGGAC 
ATTTTTGTTC AGAACCTGGT ACCCGACTGG AGAGGCATTG ATGGTAGCTT CTTTGGCATG 
TTCTCCATCT TCTTCCCTTC GGCCACTGGC ATTCTGGCAG GGGCCAACAT CTCCGGGGAC 
CTCAAGGATC CTGCTGTAGC CATCCCCAAG GGAACACTCA TGGCCATTTT CTGGACCACC 
ATCTCCTACC TTGCCATCTC AGCTACCATC GGCTCCTGTG TGGTGAGAGA TGCCTCTGGG 
GATGTGAACG ACACCATGAC TCCTGGTCCA GGCCCCTGCG AAGGGCTAGC CTGTGGCTAT 
GGCTGGAACT TCACAGAATG CTCCCAGCAG CGCAGTTGCC GCTATGGCCT CATCAACTAC 
TACCAGACCA TGAGCATGGT GTCTGCCTTC GCACCCCTGA TCACAGCCGG CATCTTTGGC 
GCCACCCTGT CTTCTGCCTT GGCCTGTCTT GTCTCGGCTG CTAAGGTCTT CCAGTGCCTG 
TGTGAAGACC AGCTGTACCC TCTGATTGGC TTTTTTGGCA AAGGCTATGG CAAGAACAGG 
GAGCCTGTGC GTGGCTACCT GCTGGCCTAT GCCATTGCTG TGGCCTTCAT CATCATTGCT 
GAACTTAACA CCATCGCCCC CATCATTTCC AACTTCTTCC TCTGCTCCTA CGCCCTCATC 
AATTTCAGCT GCTTCCATGC CTCCATCACC AACTCACCTG GATGGAGACC CTCCTTCCGA 
TACTACAGCA AGTGGGCGGC GCTGTTTGGG GCAGTGATCT CGGTGGTCAT CATGTTCCTG 
CTTACCTGGT GGGCTGCTCT CATTGCCATC GGAGTCGTCC TCTTCCTCCT GCTCTATGTG 
ATCTACAAGA AGCCAGAGGT GAACTGGGGT TCCTCAGTAC AGGCTGGCTC CTACAATCTG 
GCCCTGAGCT ACTCCGTGGG CCTCAACGAG GTGGAAGACC ACATCAAGAA CTACCGCCCC 
CAGTGCCTGG TGCTTACAGG GCCCCCCAAC TTCCGCCCAG CCCTTGTGGA CTTTGTGAGC 
ACATTTACCC AGAACCTAAG CCTAATGATT TGTGGTCATG TGCTCATTGG ACCTGGCAAG 
CAGAGAGTGC CCGAGCTCCG GCTCATCGCC AGTGGACACA CCAAGTGGCT GAACAAGAGG 
AAGATCAAGG CCTTCTACTC TGACGTCATC GCTGAGGACT TGCGCAGTGG CGTCCAGATT 
CTCATGCAGG CCTCAGGGCT TGGGAGAATG AAGCCGAACA TTCTGGTGGT TGGATTCAAG 
AGGAACTGGC AGTCTGCCCA CCCAGCCACA GTGGAGGACT ACATTGGTGT CCTGCATGAT 
GCCTTTGACT TCAACTACGG GGTTTGTGTC ATGAGGATGC GCGAGGGGCT CAATGTCTCC 
GAGGCTCTGC AGACCCACAC AACCCCAGAG GCATTGATAC AAGAGGAGCA GGCTAGCACC 
ATCTTCCAGT CGGAGCAAGG CAAGAAGACC ATTGACATCT ACTGGCTGTT CGATGATGGA 
GGTCTCACCC TCCTCATCCC CTATCTCCTG CACCGCAAGA AGAGATGGGG CAAATGCAAG 
ATCCGAGTGT TTGTTGGGGG CCAGATCAAC AGGATGGACG AAGAGAGAAA GGCGATCATC 
TCTCTGTTGA GCAAGTTCAG ACTGGGATTC CATGAAGTTC ACGTCCTTCC CGACATCAAC 
CAGAAGCCCC AGGCCGAGCA CACCAAGCGG TTTGAGGACA TGATTGCACC CTTCCGCCTC 
AATGATGGCT TCAAGGACGA GGCCACCGTC ACTGAGATGA GGAGGGACTG TCCCTGGAAG 
ATCTCAGATG AAGAGATTAA CAAGAACAGA ATTAAGTCCC TTCGGCAGGT GAGACTGAGT 
GAGATACTGC TGGATTACTC TCGGGATGCT GCTCTGATCA TCCTTACCCT GCCCATTGGA 
AGGAAGGGGA AGTGCCCCAG TTCGCTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC 
CTCAGACCTC CCGTCCTCCT CATCCGAGGC AACCAGGAGA ATGTGCTCAC CTTTTACTGC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Potassium intake modulates the thiazide-sensitive sodium-chloride cotransporter (NCC) activity via the Kir4.1 potassium channel.
Kidney international(2018 Jan)
Wang MX,Cuevas CA,Su XT,Wu P,Gao ZX,Lin DH,McCormick JA,Yang CL,Wang WH,Ellison DH

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Slc12a3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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