Skil cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Skil
Entrez Gene ID	20482
Full Name	SKI-like
Synonyms	Skir,SnoN,sno
General protein information	Preferred Names SKI-like Names ski-like protein ski-related oncogene ski/sno related
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 A3\|3 15.12 cM
Summary	This gene encodes a member of a small family of proteins that play a key role in the response of cells to extracellular growth signals. The encoded protein regulates members of the transforming growth factor beta signaling pathway. It is highly expressed in certain cancer cells, where it may have both tumor-suppressing and tumor-promoting roles. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006535427.3	XP_006535490.1	ski-like protein isoform X1
NM_001271772.1	NP_001258701.1	ski-like protein isoform c
NM_011386.3	NP_035516.2	ski-like protein isoform a
NM_001039090.2	NP_001034179.1	ski-like protein isoform b
XM_006535427.4	XP_006535490.1	ski-like protein isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SKIL	NP_001192876.1
Rattus norvegicus (Norway rat)	Skil	XP_006232299.1
Gallus gallus (chicken)	SKIL	NP_990505.1
Pan troglodytes (chimpanzee)	SKIL	XP_001163447.1
Canis lupus familiaris (dog)	SKIL	XP_545283.2
Mus musculus (house mouse)	Skil	NP_035516.2
Danio rerio (zebrafish)	LOC100007653	XP_005171386.1
	SKIL	XP_001085298.2
Homo sapiens (human)	SKIL	NP_001234937.1
Xenopus tropicalis (tropical clawed frog)	skil	XP_002931592.1

Gene Ontology
Bibliography

Related articles in PubMed

Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate.
Wang W, Song B, Anbarchian T, Shirazyan A, Sadik JE, Lyons KM
PLoS genetics12(10)e1006352(2016 Oct)

SnoN in regulation of embryonic development and tissue morphogenesis.
Zhu Q, Luo K
FEBS letters586(14)1971-6(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of SnoN is associated with partial embryonic lethality, mostly due to defects in angiogenesis in both the yolk sac and embryo body
Title: SnoN facilitates ALK1-Smad1/5 signaling during embryonic angiogenesis.

SnoN, a negative regulator of TGF-beta signaling, coordinates TGF-beta and prolactin signaling to control alveologenesis and lactogenesis.
Title: SnoN regulates mammary gland alveologenesis and onset of lactation by promoting prolactin/Stat5 signaling.

SnoN mediates a negative feedback mechanism evoked by TGF-beta to inhibit BMP signaling and, subsequently, hypertrophic maturation of chondrocytes.
Title: SnoN suppresses maturation of chondrocytes by mediating signal cross-talk between transforming growth factor-? and bone morphogenetic protein pathways.

mRNA for positive regulators of Fshb expression, such as Fos and Jun, were up-regulated at slower pulse frequencies than a number of potential negative regulators, such as the corepressors Skil, Crem, and Tgif1.
Title: Gonadotropin-releasing hormone pulse sensitivity of follicle-stimulating hormone-beta gene is mediated by differential expression of positive regulatory activator protein 1 factors and corepressors SKIL and TGIF1.

The endogenous SnoN plays a role in regulating ADAM12 expression in response to TGFbeta1.
Title: The role of SnoN in transforming growth factor beta1-induced expression of metalloprotease-disintegrin ADAM12.

The following Skil gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Skil cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16273	NM_001039090.2 Latest version!	Mus musculus SKI-like (Skil), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00
OMu17299	NM_001271772.1 Latest version!	Mus musculus SKI-like (Skil), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu15945	NM_011386.3 Latest version!	Mus musculus SKI-like (Skil), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu15945	XM_006535427.4 Latest version!	Mus musculus SKI-like (Skil), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu15945	XM_006535427.3	Mus musculus SKI-like (Skil), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Skil ( NM_001039090.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034179.1 Mus musculus SKI-like (Skil), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16273
Clone ID Related Accession (Same CDS sequence)	NM_001039090.2
Accession Version	NM_001039090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	ski-like protein isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ098423.1, AK171402.1 and AC117590.14. On Nov 20, 2012 this sequence version replaced NM_001039090.1. Transcript Variant: This variant (2, also known as DE3 or N2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154471.1, AK171402.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTGAA GAAGGAGCAC TTAGATGACT ATGGAGATGC ATCCGTGGAG 
CCGGATGGCG AGCACGCCAA GCGAAACCGT GTTTCTCTAC CTGAAACTTT AAATTTAAAC 
CCCAGTCTGA AACACACGCT GGCACAATTC CACTTAAGCA GTCAGAGCTC TTTGGGTGGA 
CCAGCAGCAT TTTCTGCTCG ATATTCCCAG GAGAGCATGT CACCGACTGT ATTCCTGCCT 
CTTCCCTCTC CTCAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAATTCATCA ATCTCAAATA ATTCTACAAG TAGAAAGAAA 
TCTGAGTCTG CTGTGTGCAG CTTAGTGAGA GGCACAAGCA AGCGTGATTC AGAAGATTCC 
TCTCCACTTC TGGTCAGAGA TGGTGAAGAC GACAAGGGGA AGATCATGGA AGACGTGATG 
AGAACCTATG TAAGACAGCA GGAGAAGCTG AACTCCATTC TGCAGAGGAA GCAGCAGCTG 
CAGATGGAGG TGGAGATGCT GAGCAGTTCA AAGGCGATGA AGGAGCTCAC TGAAGAGCAG 
CAGAATCTAC AGAAAGAGCT TGAGTCGTTA CAGAGTGAGC ATGCTCAGAG GATGGAGGAA 
TTCTATATTG AACAGAGAGA CTTAGAGAAA AAGCTGGAGC AGGTGATGCA GCAGAAATGC 
ACCTGTGACT CAACCTTGGA GAAGGACAGG GAGGCCGAGT ATGCGGCTCA GCTGGCAGAA 
CTGAGGCAAA GACTAGACCA TGCTGAGGCC GACAGGCAGG AACTCCAAGA TGAACTCAGA 
CAGGAGCGGG AGGCGAGACA GAAGCTGGAG ATGATGATAA AGGAGCTGAA GCTGCAGATT 
GGCAAGTCGT CCAAGCCCTC CAAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034179.1
CDS	729..2618
Translation	MENLQSKFSLVQGSNKKLNGMEDDGSPPVKKMMTDIHANGKTLTKVKKEHLDDYGDASVEPDGEHAKRNRVSLPETLNLNPSLKHTLAQFHLSSQSSLGGPAAFSARYSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTLLEGESISCFQVGGEKRLCLPQVLNSVLREFSLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSILPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRNGKRIQSKTDTPSGMELPSWYPVIKQEGDHVPQTHSFLHPSYYLYMCDKVVAPNVSLTSAASQSKEATKAETNSSISNNSTSRKKSESAVCSLVRGTSKRDSEDSSPLLVRDGEDDKGKIMEDVMRTYVRQQEKLNSILQRKQQLQMEVEMLSSSKAMKELTEEQQNLQKELESLQSEHAQRMEEFYIEQRDLEKKLEQVMQQKCTCDSTLEKDREAEYAAQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQIGKSSKPSKD

Target ORF information:

RefSeq Version	NM_001039090.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SKI-like (Skil), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039090.2

ORF Insert Sequence:

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTGAA GAAGGAGCAC TTAGATGACT ATGGAGATGC ATCCGTGGAG 
CCGGATGGCG AGCACGCCAA GCGAAACCGT GTTTCTCTAC CTGAAACTTT AAATTTAAAC 
CCCAGTCTGA AACACACGCT GGCACAATTC CACTTAAGCA GTCAGAGCTC TTTGGGTGGA 
CCAGCAGCAT TTTCTGCTCG ATATTCCCAG GAGAGCATGT CACCGACTGT ATTCCTGCCT 
CTTCCCTCTC CTCAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAATTCATCA ATCTCAAATA ATTCTACAAG TAGAAAGAAA 
TCTGAGTCTG CTGTGTGCAG CTTAGTGAGA GGCACAAGCA AGCGTGATTC AGAAGATTCC 
TCTCCACTTC TGGTCAGAGA TGGTGAAGAC GACAAGGGGA AGATCATGGA AGACGTGATG 
AGAACCTATG TAAGACAGCA GGAGAAGCTG AACTCCATTC TGCAGAGGAA GCAGCAGCTG 
CAGATGGAGG TGGAGATGCT GAGCAGTTCA AAGGCGATGA AGGAGCTCAC TGAAGAGCAG 
CAGAATCTAC AGAAAGAGCT TGAGTCGTTA CAGAGTGAGC ATGCTCAGAG GATGGAGGAA 
TTCTATATTG AACAGAGAGA CTTAGAGAAA AAGCTGGAGC AGGTGATGCA GCAGAAATGC 
ACCTGTGACT CAACCTTGGA GAAGGACAGG GAGGCCGAGT ATGCGGCTCA GCTGGCAGAA 
CTGAGGCAAA GACTAGACCA TGCTGAGGCC GACAGGCAGG AACTCCAAGA TGAACTCAGA 
CAGGAGCGGG AGGCGAGACA GAAGCTGGAG ATGATGATAA AGGAGCTGAA GCTGCAGATT 
GGCAAGTCGT CCAAGCCCTC CAAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skil ( NM_001271772.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258701.1 Mus musculus SKI-like (Skil), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu17299
Clone ID Related Accession (Same CDS sequence)	NM_001271772.1
Accession Version	NM_001271772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	ski-like protein isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ098423.1, AK171402.1 and AC117590.14. Transcript Variant: This variant (3) differs in the 5' UTR, lacks an in-frame portion of the 5' coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018600.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAATTCATCA ATCTCAAATA ATTCTACAAG TAGAAAGAAA 
TCTGAGTCTG CTGTGTGCAG CTTAGTGAGA GGCACAAGCA AGCGTGATTC AGAAGATTCC 
TCTCCACTTC TGGTCAGAGA TGGTGAAGAC GACAAGGGGA AGATCATGGA AGACGTGATG 
AGAACCTATG TAAGACAGCA GGAGAAGCTG AACTCCATTC TGCAGAGGAA GCAGCAGCTG 
CAGATGGAGG TGGAGATGCT GAGCAGTTCA AAGGCGATGA AGGAGCTCAC TGAAGAGCAG 
CAGAATCTAC AGAAAGAGCT TGAGTCGTTA CAGAGTGAGC ATGCTCAGAG GATGGAGGAA 
TTCTATATTG AACAGAGAGA CTTAGAGAAA AAGCTGGAGC AGGTGATGCA GCAGAAATGC 
ACCTGTGACT CAACCTTGGA GAAGGACAGG GAGGCCGAGT ATGCGGCTCA GCTGGCAGAA 
CTGAGGCAAA GACTAGACCA TGCTGAGGCC GACAGGCAGG AACTCCAAGA TGAACTCAGA 
CAGGAGCGGG AGGCGAGACA GAAGCTGGAG ATGATGATAA AGGAGCTGAA GCTGCAGATT 
GGCAAGTCGT CCAAGCCCTC CAAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258701.1
CDS	650..2299
Translation	MENLQSKFSLVQGSNKKLNGMEDDGSPPVKKMMTDIHANGKTLTKVLPGPLLIPSDSSTELTQTLLEGESISCFQVGGEKRLCLPQVLNSVLREFSLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSILPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRNGKRIQSKTDTPSGMELPSWYPVIKQEGDHVPQTHSFLHPSYYLYMCDKVVAPNVSLTSAASQSKEATKAETNSSISNNSTSRKKSESAVCSLVRGTSKRDSEDSSPLLVRDGEDDKGKIMEDVMRTYVRQQEKLNSILQRKQQLQMEVEMLSSSKAMKELTEEQQNLQKELESLQSEHAQRMEEFYIEQRDLEKKLEQVMQQKCTCDSTLEKDREAEYAAQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQIGKSSKPSKD

Target ORF information:

RefSeq Version	NM_001271772.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SKI-like (Skil), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271772.1

ORF Insert Sequence:

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAATTCATCA ATCTCAAATA ATTCTACAAG TAGAAAGAAA 
TCTGAGTCTG CTGTGTGCAG CTTAGTGAGA GGCACAAGCA AGCGTGATTC AGAAGATTCC 
TCTCCACTTC TGGTCAGAGA TGGTGAAGAC GACAAGGGGA AGATCATGGA AGACGTGATG 
AGAACCTATG TAAGACAGCA GGAGAAGCTG AACTCCATTC TGCAGAGGAA GCAGCAGCTG 
CAGATGGAGG TGGAGATGCT GAGCAGTTCA AAGGCGATGA AGGAGCTCAC TGAAGAGCAG 
CAGAATCTAC AGAAAGAGCT TGAGTCGTTA CAGAGTGAGC ATGCTCAGAG GATGGAGGAA 
TTCTATATTG AACAGAGAGA CTTAGAGAAA AAGCTGGAGC AGGTGATGCA GCAGAAATGC 
ACCTGTGACT CAACCTTGGA GAAGGACAGG GAGGCCGAGT ATGCGGCTCA GCTGGCAGAA 
CTGAGGCAAA GACTAGACCA TGCTGAGGCC GACAGGCAGG AACTCCAAGA TGAACTCAGA 
CAGGAGCGGG AGGCGAGACA GAAGCTGGAG ATGATGATAA AGGAGCTGAA GCTGCAGATT 
GGCAAGTCGT CCAAGCCCTC CAAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skil ( NM_011386.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035516.2 Mus musculus SKI-like (Skil), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15945
Clone ID Related Accession (Same CDS sequence)	XM_006535427.3 , NM_011386.3 , XM_006535427.4
Accession Version	NM_011386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	ski-like protein isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ098423.1, AK171402.1, CB519078.1 and AC117590.14. On Nov 20, 2012 this sequence version replaced NM_011386.2. Transcript Variant: This variant (1, also known as FL) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.52878.1, SRR7652917.1095871.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTGAA GAAGGAGCAC TTAGATGACT ATGGAGATGC ATCCGTGGAG 
CCGGATGGCG AGCACGCCAA GCGAAACCGT GTTTCTCTAC CTGAAACTTT AAATTTAAAC 
CCCAGTCTGA AACACACGCT GGCACAATTC CACTTAAGCA GTCAGAGCTC TTTGGGTGGA 
CCAGCAGCAT TTTCTGCTCG ATATTCCCAG GAGAGCATGT CACCGACTGT ATTCCTGCCT 
CTTCCCTCTC CTCAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAGTAAGTCC ACCTCCAAAC AGTCAGAAAA GCCTCATGAG 
AGTAGTCAGC ATCAAAAAAC AGTGTCTTAC CCAGACGTCT CACTTGAGGA GCAGGAGAAA 
ATGGATTTAA AAACCAGTAG AGAATTATAC AGTTGTTTAG ATTCATCAAT CTCAAATAAT 
TCTACAAGTA GAAAGAAATC TGAGTCTGCT GTGTGCAGCT TAGTGAGAGG CACAAGCAAG 
CGTGATTCAG AAGATTCCTC TCCACTTCTG GTCAGAGATG GTGAAGACGA CAAGGGGAAG 
ATCATGGAAG ACGTGATGAG AACCTATGTA AGACAGCAGG AGAAGCTGAA CTCCATTCTG 
CAGAGGAAGC AGCAGCTGCA GATGGAGGTG GAGATGCTGA GCAGTTCAAA GGCGATGAAG 
GAGCTCACTG AAGAGCAGCA GAATCTACAG AAAGAGCTTG AGTCGTTACA GAGTGAGCAT 
GCTCAGAGGA TGGAGGAATT CTATATTGAA CAGAGAGACT TAGAGAAAAA GCTGGAGCAG 
GTGATGCAGC AGAAATGCAC CTGTGACTCA ACCTTGGAGA AGGACAGGGA GGCCGAGTAT 
GCGGCTCAGC TGGCAGAACT GAGGCAAAGA CTAGACCATG CTGAGGCCGA CAGGCAGGAA 
CTCCAAGATG AACTCAGACA GGAGCGGGAG GCGAGACAGA AGCTGGAGAT GATGATAAAG 
GAGCTGAAGC TGCAGATTGG CAAGTCGTCC AAGCCCTCCA AGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035516.2
CDS	729..2756
Translation	MENLQSKFSLVQGSNKKLNGMEDDGSPPVKKMMTDIHANGKTLTKVKKEHLDDYGDASVEPDGEHAKRNRVSLPETLNLNPSLKHTLAQFHLSSQSSLGGPAAFSARYSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTLLEGESISCFQVGGEKRLCLPQVLNSVLREFSLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSILPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRNGKRIQSKTDTPSGMELPSWYPVIKQEGDHVPQTHSFLHPSYYLYMCDKVVAPNVSLTSAASQSKEATKAETSKSTSKQSEKPHESSQHQKTVSYPDVSLEEQEKMDLKTSRELYSCLDSSISNNSTSRKKSESAVCSLVRGTSKRDSEDSSPLLVRDGEDDKGKIMEDVMRTYVRQQEKLNSILQRKQQLQMEVEMLSSSKAMKELTEEQQNLQKELESLQSEHAQRMEEFYIEQRDLEKKLEQVMQQKCTCDSTLEKDREAEYAAQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQIGKSSKPSKD

Target ORF information:

RefSeq Version	NM_011386.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SKI-like (Skil), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011386.3

ORF Insert Sequence:

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTGAA GAAGGAGCAC TTAGATGACT ATGGAGATGC ATCCGTGGAG 
CCGGATGGCG AGCACGCCAA GCGAAACCGT GTTTCTCTAC CTGAAACTTT AAATTTAAAC 
CCCAGTCTGA AACACACGCT GGCACAATTC CACTTAAGCA GTCAGAGCTC TTTGGGTGGA 
CCAGCAGCAT TTTCTGCTCG ATATTCCCAG GAGAGCATGT CACCGACTGT ATTCCTGCCT 
CTTCCCTCTC CTCAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAGTAAGTCC ACCTCCAAAC AGTCAGAAAA GCCTCATGAG 
AGTAGTCAGC ATCAAAAAAC AGTGTCTTAC CCAGACGTCT CACTTGAGGA GCAGGAGAAA 
ATGGATTTAA AAACCAGTAG AGAATTATAC AGTTGTTTAG ATTCATCAAT CTCAAATAAT 
TCTACAAGTA GAAAGAAATC TGAGTCTGCT GTGTGCAGCT TAGTGAGAGG CACAAGCAAG 
CGTGATTCAG AAGATTCCTC TCCACTTCTG GTCAGAGATG GTGAAGACGA CAAGGGGAAG 
ATCATGGAAG ACGTGATGAG AACCTATGTA AGACAGCAGG AGAAGCTGAA CTCCATTCTG 
CAGAGGAAGC AGCAGCTGCA GATGGAGGTG GAGATGCTGA GCAGTTCAAA GGCGATGAAG 
GAGCTCACTG AAGAGCAGCA GAATCTACAG AAAGAGCTTG AGTCGTTACA GAGTGAGCAT 
GCTCAGAGGA TGGAGGAATT CTATATTGAA CAGAGAGACT TAGAGAAAAA GCTGGAGCAG 
GTGATGCAGC AGAAATGCAC CTGTGACTCA ACCTTGGAGA AGGACAGGGA GGCCGAGTAT 
GCGGCTCAGC TGGCAGAACT GAGGCAAAGA CTAGACCATG CTGAGGCCGA CAGGCAGGAA 
CTCCAAGATG AACTCAGACA GGAGCGGGAG GCGAGACAGA AGCTGGAGAT GATGATAAAG 
GAGCTGAAGC TGCAGATTGG CAAGTCGTCC AAGCCCTCCA AGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skil ( XM_006535427.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535490.1 Mus musculus SKI-like (Skil), transcript variant X1, mRNA.

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CloneID	OMu15945
Clone ID Related Accession (Same CDS sequence)	XM_006535427.3 , NM_011386.3 , XM_006535427.4
Accession Version	XM_006535427.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ski-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTGAA GAAGGAGCAC TTAGATGACT ATGGAGATGC ATCCGTGGAG 
CCGGATGGCG AGCACGCCAA GCGAAACCGT GTTTCTCTAC CTGAAACTTT AAATTTAAAC 
CCCAGTCTGA AACACACGCT GGCACAATTC CACTTAAGCA GTCAGAGCTC TTTGGGTGGA 
CCAGCAGCAT TTTCTGCTCG ATATTCCCAG GAGAGCATGT CACCGACTGT ATTCCTGCCT 
CTTCCCTCTC CTCAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAGTAAGTCC ACCTCCAAAC AGTCAGAAAA GCCTCATGAG 
AGTAGTCAGC ATCAAAAAAC AGTGTCTTAC CCAGACGTCT CACTTGAGGA GCAGGAGAAA 
ATGGATTTAA AAACCAGTAG AGAATTATAC AGTTGTTTAG ATTCATCAAT CTCAAATAAT 
TCTACAAGTA GAAAGAAATC TGAGTCTGCT GTGTGCAGCT TAGTGAGAGG CACAAGCAAG 
CGTGATTCAG AAGATTCCTC TCCACTTCTG GTCAGAGATG GTGAAGACGA CAAGGGGAAG 
ATCATGGAAG ACGTGATGAG AACCTATGTA AGACAGCAGG AGAAGCTGAA CTCCATTCTG 
CAGAGGAAGC AGCAGCTGCA GATGGAGGTG GAGATGCTGA GCAGTTCAAA GGCGATGAAG 
GAGCTCACTG AAGAGCAGCA GAATCTACAG AAAGAGCTTG AGTCGTTACA GAGTGAGCAT 
GCTCAGAGGA TGGAGGAATT CTATATTGAA CAGAGAGACT TAGAGAAAAA GCTGGAGCAG 
GTGATGCAGC AGAAATGCAC CTGTGACTCA ACCTTGGAGA AGGACAGGGA GGCCGAGTAT 
GCGGCTCAGC TGGCAGAACT GAGGCAAAGA CTAGACCATG CTGAGGCCGA CAGGCAGGAA 
CTCCAAGATG AACTCAGACA GGAGCGGGAG GCGAGACAGA AGCTGGAGAT GATGATAAAG 
GAGCTGAAGC TGCAGATTGG CAAGTCGTCC AAGCCCTCCA AGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535490.1
CDS	416..2443
Translation	MENLQSKFSLVQGSNKKLNGMEDDGSPPVKKMMTDIHANGKTLTKVKKEHLDDYGDASVEPDGEHAKRNRVSLPETLNLNPSLKHTLAQFHLSSQSSLGGPAAFSARYSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTLLEGESISCFQVGGEKRLCLPQVLNSVLREFSLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSILPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRNGKRIQSKTDTPSGMELPSWYPVIKQEGDHVPQTHSFLHPSYYLYMCDKVVAPNVSLTSAASQSKEATKAETSKSTSKQSEKPHESSQHQKTVSYPDVSLEEQEKMDLKTSRELYSCLDSSISNNSTSRKKSESAVCSLVRGTSKRDSEDSSPLLVRDGEDDKGKIMEDVMRTYVRQQEKLNSILQRKQQLQMEVEMLSSSKAMKELTEEQQNLQKELESLQSEHAQRMEEFYIEQRDLEKKLEQVMQQKCTCDSTLEKDREAEYAAQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQIGKSSKPSKD

Target ORF information:

RefSeq Version	XM_006535427.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SKI-like (Skil), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535427.3

ORF Insert Sequence:

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTGAA GAAGGAGCAC TTAGATGACT ATGGAGATGC ATCCGTGGAG 
CCGGATGGCG AGCACGCCAA GCGAAACCGT GTTTCTCTAC CTGAAACTTT AAATTTAAAC 
CCCAGTCTGA AACACACGCT GGCACAATTC CACTTAAGCA GTCAGAGCTC TTTGGGTGGA 
CCAGCAGCAT TTTCTGCTCG ATATTCCCAG GAGAGCATGT CACCGACTGT ATTCCTGCCT 
CTTCCCTCTC CTCAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAGTAAGTCC ACCTCCAAAC AGTCAGAAAA GCCTCATGAG 
AGTAGTCAGC ATCAAAAAAC AGTGTCTTAC CCAGACGTCT CACTTGAGGA GCAGGAGAAA 
ATGGATTTAA AAACCAGTAG AGAATTATAC AGTTGTTTAG ATTCATCAAT CTCAAATAAT 
TCTACAAGTA GAAAGAAATC TGAGTCTGCT GTGTGCAGCT TAGTGAGAGG CACAAGCAAG 
CGTGATTCAG AAGATTCCTC TCCACTTCTG GTCAGAGATG GTGAAGACGA CAAGGGGAAG 
ATCATGGAAG ACGTGATGAG AACCTATGTA AGACAGCAGG AGAAGCTGAA CTCCATTCTG 
CAGAGGAAGC AGCAGCTGCA GATGGAGGTG GAGATGCTGA GCAGTTCAAA GGCGATGAAG 
GAGCTCACTG AAGAGCAGCA GAATCTACAG AAAGAGCTTG AGTCGTTACA GAGTGAGCAT 
GCTCAGAGGA TGGAGGAATT CTATATTGAA CAGAGAGACT TAGAGAAAAA GCTGGAGCAG 
GTGATGCAGC AGAAATGCAC CTGTGACTCA ACCTTGGAGA AGGACAGGGA GGCCGAGTAT 
GCGGCTCAGC TGGCAGAACT GAGGCAAAGA CTAGACCATG CTGAGGCCGA CAGGCAGGAA 
CTCCAAGATG AACTCAGACA GGAGCGGGAG GCGAGACAGA AGCTGGAGAT GATGATAAAG 
GAGCTGAAGC TGCAGATTGG CAAGTCGTCC AAGCCCTCCA AGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skil ( XM_006535427.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535490.1 Mus musculus SKI-like (Skil), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15945
Clone ID Related Accession (Same CDS sequence)	XM_006535427.3 , NM_011386.3 , XM_006535427.4
Accession Version	XM_006535427.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ski-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535427.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTGAA GAAGGAGCAC TTAGATGACT ATGGAGATGC ATCCGTGGAG 
CCGGATGGCG AGCACGCCAA GCGAAACCGT GTTTCTCTAC CTGAAACTTT AAATTTAAAC 
CCCAGTCTGA AACACACGCT GGCACAATTC CACTTAAGCA GTCAGAGCTC TTTGGGTGGA 
CCAGCAGCAT TTTCTGCTCG ATATTCCCAG GAGAGCATGT CACCGACTGT ATTCCTGCCT 
CTTCCCTCTC CTCAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAGTAAGTCC ACCTCCAAAC AGTCAGAAAA GCCTCATGAG 
AGTAGTCAGC ATCAAAAAAC AGTGTCTTAC CCAGACGTCT CACTTGAGGA GCAGGAGAAA 
ATGGATTTAA AAACCAGTAG AGAATTATAC AGTTGTTTAG ATTCATCAAT CTCAAATAAT 
TCTACAAGTA GAAAGAAATC TGAGTCTGCT GTGTGCAGCT TAGTGAGAGG CACAAGCAAG 
CGTGATTCAG AAGATTCCTC TCCACTTCTG GTCAGAGATG GTGAAGACGA CAAGGGGAAG 
ATCATGGAAG ACGTGATGAG AACCTATGTA AGACAGCAGG AGAAGCTGAA CTCCATTCTG 
CAGAGGAAGC AGCAGCTGCA GATGGAGGTG GAGATGCTGA GCAGTTCAAA GGCGATGAAG 
GAGCTCACTG AAGAGCAGCA GAATCTACAG AAAGAGCTTG AGTCGTTACA GAGTGAGCAT 
GCTCAGAGGA TGGAGGAATT CTATATTGAA CAGAGAGACT TAGAGAAAAA GCTGGAGCAG 
GTGATGCAGC AGAAATGCAC CTGTGACTCA ACCTTGGAGA AGGACAGGGA GGCCGAGTAT 
GCGGCTCAGC TGGCAGAACT GAGGCAAAGA CTAGACCATG CTGAGGCCGA CAGGCAGGAA 
CTCCAAGATG AACTCAGACA GGAGCGGGAG GCGAGACAGA AGCTGGAGAT GATGATAAAG 
GAGCTGAAGC TGCAGATTGG CAAGTCGTCC AAGCCCTCCA AGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535490.1
CDS	153..2180
Translation	MENLQSKFSLVQGSNKKLNGMEDDGSPPVKKMMTDIHANGKTLTKVKKEHLDDYGDASVEPDGEHAKRNRVSLPETLNLNPSLKHTLAQFHLSSQSSLGGPAAFSARYSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTLLEGESISCFQVGGEKRLCLPQVLNSVLREFSLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSILPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRNGKRIQSKTDTPSGMELPSWYPVIKQEGDHVPQTHSFLHPSYYLYMCDKVVAPNVSLTSAASQSKEATKAETSKSTSKQSEKPHESSQHQKTVSYPDVSLEEQEKMDLKTSRELYSCLDSSISNNSTSRKKSESAVCSLVRGTSKRDSEDSSPLLVRDGEDDKGKIMEDVMRTYVRQQEKLNSILQRKQQLQMEVEMLSSSKAMKELTEEQQNLQKELESLQSEHAQRMEEFYIEQRDLEKKLEQVMQQKCTCDSTLEKDREAEYAAQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQIGKSSKPSKD

Target ORF information:

RefSeq Version	XM_006535427.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SKI-like (Skil), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535427.4

ORF Insert Sequence:

ATGGAAAACC TCCAGTCTAA GTTCTCCTTA GTTCAGGGTT CAAATAAAAA GCTGAACGGC 
ATGGAGGATG ATGGCAGTCC TCCTGTGAAA AAAATGATGA CCGACATTCA TGCCAATGGG 
AAAACCCTGA CCAAGGTGAA GAAGGAGCAC TTAGATGACT ATGGAGATGC ATCCGTGGAG 
CCGGATGGCG AGCACGCCAA GCGAAACCGT GTTTCTCTAC CTGAAACTTT AAATTTAAAC 
CCCAGTCTGA AACACACGCT GGCACAATTC CACTTAAGCA GTCAGAGCTC TTTGGGTGGA 
CCAGCAGCAT TTTCTGCTCG ATATTCCCAG GAGAGCATGT CACCGACTGT ATTCCTGCCT 
CTTCCCTCTC CTCAGGTTCT TCCCGGCCCT CTTCTCATTC CTTCTGATAG CTCCACAGAG 
CTCACTCAGA CTCTTTTGGA AGGGGAGTCT ATTTCTTGTT TTCAAGTTGG AGGAGAAAAG 
AGACTCTGTT TGCCCCAAGT CTTAAACTCT GTCCTCCGAG AATTTTCACT CCAGCAGATA 
AACACAGTAT GTGATGAGCT GTACATCTAT TGTTCAAGGT GTACTTCAGA CCAGCTTCAT 
ATCTTAAAGG TCTTAGGGAT ACTCCCATTT AATGCTCCAT CCTGTGGGCT GATCACGTTG 
ACCGATGCAC AAAGACTCTG TAATGCTTTA CTGCGGCCAC GAACTTTTCC TCAAAATGGT 
AGCATACTTC CTGCTAAAAG CTCTCTGGCC CAGTTGAAGG AAACTGGCAG TGCCTTTGAA 
GTGGAACATG AATGCTTGGG CAAATGTCAG GGTCTCTTTG CGCCTCAGTT TTATGTTCAG 
CCCGACGCTC CCTGTATCCA GTGCCTGGAG TGCTGTGGAA TGTTTGCTCC CCAGACGTTC 
GTCATGCATT CGCACAGATC CCCTGACAAG AGAACTTGCC ATTGGGGCTT TGAGTCAGCC 
AAGTGGCACT GTTACCTTCA TGTGAACCAA AAATACCTAG GGACACCCGA AGAAAAGAAA 
CTGAAGATAA TTTTAGAAGA AATGAAGGAG AAGTTTAGCA TGAGAAATGG AAAGAGAATC 
CAATCAAAGA CAGATACACC ATCGGGAATG GAATTGCCAT CATGGTATCC TGTTATAAAA 
CAGGAAGGTG ACCATGTTCC CCAGACACAT TCATTTTTAC ACCCCAGCTA CTACCTGTAC 
ATGTGTGACA AAGTGGTTGC ACCGAATGTG TCGCTCACAT CTGCTGCGTC CCAGTCTAAA 
GAGGCCACCA AGGCAGAGAC AAGTAAGTCC ACCTCCAAAC AGTCAGAAAA GCCTCATGAG 
AGTAGTCAGC ATCAAAAAAC AGTGTCTTAC CCAGACGTCT CACTTGAGGA GCAGGAGAAA 
ATGGATTTAA AAACCAGTAG AGAATTATAC AGTTGTTTAG ATTCATCAAT CTCAAATAAT 
TCTACAAGTA GAAAGAAATC TGAGTCTGCT GTGTGCAGCT TAGTGAGAGG CACAAGCAAG 
CGTGATTCAG AAGATTCCTC TCCACTTCTG GTCAGAGATG GTGAAGACGA CAAGGGGAAG 
ATCATGGAAG ACGTGATGAG AACCTATGTA AGACAGCAGG AGAAGCTGAA CTCCATTCTG 
CAGAGGAAGC AGCAGCTGCA GATGGAGGTG GAGATGCTGA GCAGTTCAAA GGCGATGAAG 
GAGCTCACTG AAGAGCAGCA GAATCTACAG AAAGAGCTTG AGTCGTTACA GAGTGAGCAT 
GCTCAGAGGA TGGAGGAATT CTATATTGAA CAGAGAGACT TAGAGAAAAA GCTGGAGCAG 
GTGATGCAGC AGAAATGCAC CTGTGACTCA ACCTTGGAGA AGGACAGGGA GGCCGAGTAT 
GCGGCTCAGC TGGCAGAACT GAGGCAAAGA CTAGACCATG CTGAGGCCGA CAGGCAGGAA 
CTCCAAGATG AACTCAGACA GGAGCGGGAG GCGAGACAGA AGCTGGAGAT GATGATAAAG 
GAGCTGAAGC TGCAGATTGG CAAGTCGTCC AAGCCCTCCA AGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate.
PLoS genetics12(10)e1006352(2016 Oct)
Wang W,Song B,Anbarchian T,Shirazyan A,Sadik JE,Lyons KM

SnoN in regulation of embryonic development and tissue morphogenesis.
FEBS letters586(14)1971-6(2012 Jul)
Zhu Q,Luo K

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Skil cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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