Siah2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Siah2
Entrez Gene ID	20439
Full Name	siah E3 ubiquitin protein ligase 2
Synonyms	AA415433,Sinh2
General protein information	Preferred Names siah E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase SIAH2 RING-type E3 ubiquitin transferase SIAH2 mSiah2 seven in absentia 2 seven in absentia homolog 2 siah-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 D\|3 28.68 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009174.3	NP_033200.2	E3 ubiquitin-protein ligase SIAH2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIAH2	XP_516819.2
	SIAH2	XP_001107642.1
Canis lupus familiaris (dog)	SIAH2	XP_005634711.1
Mus musculus (house mouse)	Siah2	NP_033200.2
Rattus norvegicus (Norway rat)	Siah2	NP_604452.1
Gallus gallus (chicken)	SIAH2	XP_426719.2
Danio rerio (zebrafish)	siah2l	NP_956721.1
Bos taurus (cattle)	SIAH2	NP_001193983.1
Homo sapiens (human)	SIAH2	NP_005058.3
Xenopus tropicalis (tropical clawed frog)	siah2	NP_001095281.1

Gene Ontology
Bibliography

Related articles in PubMed

E3 ubiquitin ligases SIAH1/2 regulate hypoxia-inducible factor-1 (HIF-1)-mediated Th17 cell differentiation.
Matsui-Hasumi A, Sato Y, Uto-Konomi A, Yamashita S, Uehori J, Yoshimura A, Yamashita M, Asahara H, Suzuki S, Kubo M
International immunology29(3)133-143(2017 03)

Siah2 Protein Mediates Early Events in Commitment to an Adipogenic Pathway.
Kilroy G, Burk DH, Floyd ZE
The Journal of biological chemistry291(53)27289-27297(2016 12)

Zeb1 controls neuron differentiation and germinal zone exit by a mesenchymal-epithelial-like transition.
Singh S, Howell D, Trivedi N, Kessler K, Ong T, Rosmaninho P, Raposo AA, Robinson G, Roussel MF, Castro DS, Solecki DJ
eLife5(2016 05)

Akap1 Deficiency Promotes Mitochondrial Aberrations and Exacerbates Cardiac Injury Following Permanent Coronary Ligation via Enhanced Mitophagy and Apoptosis.
Schiattarella GG, Cattaneo F, Pironti G, Magliulo F, Carotenuto G, Pirozzi M, Polishchuk R, Borzacchiello D, Paolillo R, Oliveti M, Boccella N, Avvedimento M, Sepe M, Lombardi A, Busiello RA, Trimarco B, Esposito G, Feliciello A, Perrino C
PloS one11(5)e0154076(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that SIAH2 plays a pivotal role to promote Th17 cell differentiation through maintaining the stability of HIF-1alpha protein.
Title: E3 ubiquitin ligases SIAH1/2 regulate hypoxia-inducible factor-1 (HIF-1)-mediated Th17 cell differentiation.

High Siah2 expression is associated with increased adipogenesis.
Title: Siah2 Protein Mediates Early Events in Commitment to an Adipogenic Pathway.

Siah2(-/-) endothelial cells have an intact hypoxic signalling pathway, including Hif-1alpha stabilisation and gene expression, the first report of a tissue or cell lineage in which the loss of Siah2 does not seem to impact hypoxic response signaling.
Title: Loss of Siah2 does not impact angiogenic potential of murine endothelial cells.

Siah2 links adipocyte hypertrophy with adipocyte dysfunction and recruitment of proinflammatory immune cells to adipose tissue.
Title: The ubiquitin ligase Siah2 regulates obesity-induced adipose tissue inflammation.

A catalysis-independent role for AKR1C3 on AR activity via Siah2 has been identified.
Title: The Steroidogenic Enzyme AKR1C3 Regulates Stability of the Ubiquitin Ligase Siah2 in Prostate Cancer Cells.

The following Siah2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Siah2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16137	NM_009174.3 Latest version!	Mus musculus siah E3 ubiquitin protein ligase 2 (Siah2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Siah2 ( NM_009174.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033200.2 Mus musculus siah E3 ubiquitin protein ligase 2 (Siah2), mRNA.

Price & Availability↑

CloneID	OMu16137
Clone ID Related Accession (Same CDS sequence)	NM_009174.3
Accession Version	NM_009174.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SIAH2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC119873.38. On Nov 17, 2006 this sequence version replaced NM_009174.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058400.1, AK149316.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164136 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCGCC CGTCCTCCAC CGGCCCCAGC GCTAACAAAC CCTGCAGCAA GCAGCCGCCG 
CCGCCTCAGA CCCCGCACGC CCCGTCCCCG GCTGCGCCCC CGGCCGCCGC CACCATCTCG 
GCAGCGGGCC CCGGCTCGTC CGCGGTGCCC GCCGCGGCAG CGGTGATCTC AGGCCCCGGA 
GCCGGCGGCG GGGCCGACCC GGTGTCCCCG CAGCACCACG AGCTGACCTC GCTCTTCGAG 
TGCCCGGTCT GCTTTGACTA TGTCCTGCCC CCCATTCTGC AGTGCCAGGC CGGGCACCTG 
GTGTGTAACC AATGCCGCCA GAAGTTAAGC TGCTGCCCGA CGTGCAGGGG CGCCCTAACG 
CCCAGCATCA GGAACCTGGC TATGGAGAAG GTCGCCTCGG CAGTTCTGTT TCCCTGTAAG 
TATGCTACCA CGGGCTGTTC CCTGACTCTA CACCATACAG AGAAACCAGA GCATGAAGAC 
ATCTGTGAAT ACCGTCCTTA TTCCTGTCCT TGTCCTGGTG CGTCCTGCAA GTGGCAGGGA 
TCCCTGGAAG CTGTGATGTC CCATCTCATG CATGCCCACA AGAGTATCAC TACCCTTCAG 
GGAGAGGACA TAGTCTTTCT AGCTACAGAC ATTAACCTGC CAGGGGCTGT GGACTGGGTG 
ATGATGCAGT CCTGTTTTGG CCACCACTTC ATGCTGGTAC TTGAAAAGCA AGAGAAGTAC 
GAAGGCCACC AGCAGTTCTT TGCCATCGTC CTCCTCATTG GCACCCGAAA GCAAGCTGAG 
AACTTTGCCT ACAGACTGGA GTTGAATGGG AACCGGAGGA GACTGACCTG GGAGGCCACA 
CCTCGGTCCA TTCATGATGG CGTGGCTGCA GCCATCATGA ACAGTGACTG TCTTGTTTTT 
GACACAGCCA TAGCACATCT CTTTGCAGAT AATGGGAACC TTGGAATCAA TGTCACGATA 
TCTACATGCT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033200.2
CDS	574..1551
Translation	MSRPSSTGPSANKPCSKQPPPPQTPHAPSPAAPPAAATISAAGPGSSAVPAAAAVISGPGAGGGADPVSPQHHELTSLFECPVCFDYVLPPILQCQAGHLVCNQCRQKLSCCPTCRGALTPSIRNLAMEKVASAVLFPCKYATTGCSLTLHHTEKPEHEDICEYRPYSCPCPGASCKWQGSLEAVMSHLMHAHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGHHFMLVLEKQEKYEGHQQFFAIVLLIGTRKQAENFAYRLELNGNRRRLTWEATPRSIHDGVAAAIMNSDCLVFDTAIAHLFADNGNLGINVTISTCCQ

Target ORF information:

RefSeq Version	NM_009174.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus siah E3 ubiquitin protein ligase 2 (Siah2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009174.3

ORF Insert Sequence:

ATGAGCCGCC CGTCCTCCAC CGGCCCCAGC GCTAACAAAC CCTGCAGCAA GCAGCCGCCG 
CCGCCTCAGA CCCCGCACGC CCCGTCCCCG GCTGCGCCCC CGGCCGCCGC CACCATCTCG 
GCAGCGGGCC CCGGCTCGTC CGCGGTGCCC GCCGCGGCAG CGGTGATCTC AGGCCCCGGA 
GCCGGCGGCG GGGCCGACCC GGTGTCCCCG CAGCACCACG AGCTGACCTC GCTCTTCGAG 
TGCCCGGTCT GCTTTGACTA TGTCCTGCCC CCCATTCTGC AGTGCCAGGC CGGGCACCTG 
GTGTGTAACC AATGCCGCCA GAAGTTAAGC TGCTGCCCGA CGTGCAGGGG CGCCCTAACG 
CCCAGCATCA GGAACCTGGC TATGGAGAAG GTCGCCTCGG CAGTTCTGTT TCCCTGTAAG 
TATGCTACCA CGGGCTGTTC CCTGACTCTA CACCATACAG AGAAACCAGA GCATGAAGAC 
ATCTGTGAAT ACCGTCCTTA TTCCTGTCCT TGTCCTGGTG CGTCCTGCAA GTGGCAGGGA 
TCCCTGGAAG CTGTGATGTC CCATCTCATG CATGCCCACA AGAGTATCAC TACCCTTCAG 
GGAGAGGACA TAGTCTTTCT AGCTACAGAC ATTAACCTGC CAGGGGCTGT GGACTGGGTG 
ATGATGCAGT CCTGTTTTGG CCACCACTTC ATGCTGGTAC TTGAAAAGCA AGAGAAGTAC 
GAAGGCCACC AGCAGTTCTT TGCCATCGTC CTCCTCATTG GCACCCGAAA GCAAGCTGAG 
AACTTTGCCT ACAGACTGGA GTTGAATGGG AACCGGAGGA GACTGACCTG GGAGGCCACA 
CCTCGGTCCA TTCATGATGG CGTGGCTGCA GCCATCATGA ACAGTGACTG TCTTGTTTTT 
GACACAGCCA TAGCACATCT CTTTGCAGAT AATGGGAACC TTGGAATCAA TGTCACGATA 
TCTACATGCT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

E3 ubiquitin ligases SIAH1/2 regulate hypoxia-inducible factor-1 (HIF-1)-mediated Th17 cell differentiation.
International immunology29(3)133-143(2017 03)
Matsui-Hasumi A,Sato Y,Uto-Konomi A,Yamashita S,Uehori J,Yoshimura A,Yamashita M,Asahara H,Suzuki S,Kubo M

Siah2 Protein Mediates Early Events in Commitment to an Adipogenic Pathway.
The Journal of biological chemistry291(53)27289-27297(2016 12)
Kilroy G,Burk DH,Floyd ZE

Zeb1 controls neuron differentiation and germinal zone exit by a mesenchymal-epithelial-like transition.
eLife5(2016 05)
Singh S,Howell D,Trivedi N,Kessler K,Ong T,Rosmaninho P,Raposo AA,Robinson G,Roussel MF,Castro DS,Solecki DJ

Akap1 Deficiency Promotes Mitochondrial Aberrations and Exacerbates Cardiac Injury Following Permanent Coronary Ligation via Enhanced Mitophagy and Apoptosis.
PloS one11(5)e0154076(2016)
Schiattarella GG,Cattaneo F,Pironti G,Magliulo F,Carotenuto G,Pirozzi M,Polishchuk R,Borzacchiello D,Paolillo R,Oliveti M,Boccella N,Avvedimento M,Sepe M,Lombardi A,Busiello RA,Trimarco B,Esposito G,Feliciello A,Perrino C

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Siah2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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