Siah1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Siah1a
Entrez Gene ID	20437
Full Name	siah E3 ubiquitin protein ligase 1A
Synonyms	AA982064,AI853500,Sinh1a
General protein information	Preferred Names siah E3 ubiquitin protein ligase 1A Names E3 ubiquitin-protein ligase SIAH1A RING-type E3 ubiquitin transferase SIAH1A mSiah-1a seven in absentia 1A seven in absentia homolog 1a siah-1a
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 C3\|8 42.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530784.3	XP_006530847.2	E3 ubiquitin-protein ligase SIAH1A isoform X1
XM_006530784.4	XP_006530847.3	E3 ubiquitin-protein ligase SIAH1A isoform X1
NM_009172.2	NP_033198.1	E3 ubiquitin-protein ligase SIAH1A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Siah1a	NP_033198.1
Rattus norvegicus (Norway rat)	Siah1a	NP_543181.2
Gallus gallus (chicken)	SIAH1	XP_414105.2
Oryza sativa (rice)	Os03g0356414	NP_001173420.1
Homo sapiens (human)	SIAH1	NP_001006611.1
Pan troglodytes (chimpanzee)	SIAH1	NP_001233288.1
	SIAH1	XP_001114300.1
Canis lupus familiaris (dog)	SIAH1	XP_003638979.1
Drosophila melanogaster (fruit fly)	sina	NP_476725.1
Bos taurus (cattle)	SIAH1	NP_001180122.1
Caenorhabditis elegans (roundworm)	siah-1	NP_500409.1
Xenopus tropicalis (tropical clawed frog)	siah1	NP_001015836.1
Danio rerio (zebrafish)	siah1	NP_955815.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006127	XP_001688791.1
Oryza sativa (rice)	Os07g0659800	NP_001060528.1

Gene Ontology
Bibliography

Related articles in PubMed

HIF-1? coordinates epigenetic activation of SIAH1 in hepatocytes in response to nutritional stress.
Fan Z, Li Z, Yang Y, Liu S, Guo J, Xu Y
Biochimica et biophysica acta1860(10)1037-1046(2017 10)

Central role of SIAH inhibition in DCC-dependent cardioprotection provoked by netrin-1/NO.
Li Q, Wang P, Ye K, Cai H
Proceedings of the National Academy of Sciences of the United States of America112(3)899-904(2015 Jan)

Fine tuning of the UPR by the ubiquitin ligases Siah1/2.
Scortegagna M, Kim H, Li JL, Yao H, Brill LM, Han J, Lau E, Bowtell D, Haddad G, Kaufman RJ, Ronai ZA
PLoS genetics10(5)e1004348(2014 May)

S-nitrosylation of B23/nucleophosmin by GAPDH protects cells from the SIAH1-GAPDH death cascade.
Lee SB, Kim CK, Lee KH, Ahn JY
The Journal of cell biology199(1)65-76(2012 Oct)

miR-135A regulates preimplantation embryo development through down-regulation of E3 Ubiquitin Ligase Seven In Absentia Homolog 1A (SIAH1A) expression.
Pang RT, Liu WM, Leung CO, Ye TM, Kwan PC, Lee KF, Yeung WS
PloS one6(11)e27878(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Depletion of KDM3A coincided with the loss of SIAH1 induction and the accumulation of dimethylated H3K9 surrounding the SIAH1 promoter. Interestingly, KDM3A expression was also up-regulated by nutritional stress in a HIF-1alpha dependent manner.
Title: HIF-1? coordinates epigenetic activation of SIAH1 in hepatocytes in response to nutritional stress.

Combination of netrin-1 and SIAH RNAi may prove to be a substantially effective therapy for myocardial infarction.
Title: Central role of SIAH inhibition in DCC-dependent cardioprotection provoked by netrin-1/NO.

The B23 regulates neuronal survival by preventing SIAH1-GAPDH death signaling under stress-induced conditions in the brain.
Title: S-nitrosylation of B23/nucleophosmin by GAPDH protects cells from the SIAH1-GAPDH death cascade.

Siah1 protein is required for glucose limitation-induced Cyclin-Dependent Kinase Inhibitor p27 degradation.
Title: Siah1/SIP regulates p27(kip1) stability and cell migration under metabolic stress.

The present study demonstrated for the first time that zygotic specific miRNA modulates the first cell cleavage through regulating expression of Siah1a.
Title: miR-135A regulates preimplantation embryo development through down-regulation of E3 Ubiquitin Ligase Seven In Absentia Homolog 1A (SIAH1A) expression.

The following Siah1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Siah1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03662	NM_009172.2 Latest version!	Mus musculus siah E3 ubiquitin protein ligase 1A (Siah1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu03662	XM_006530784.3 Latest version!	Mus musculus seven in absentia 1A (Siah1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu41299	XM_006530784.4 Latest version!	Mus musculus siah E3 ubiquitin protein ligase 1A (Siah1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Siah1a ( NM_009172.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033198.1 Mus musculus siah E3 ubiquitin protein ligase 1A (Siah1a), mRNA.

Price & Availability↑

CloneID	OMu03662
Clone ID Related Accession (Same CDS sequence)	XM_006530784.3 , NM_009172.2
Accession Version	NM_009172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SIAH1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC046317.1, AC142211.3, BE634662.1 and AA420282.1. On Aug 28, 2009 this sequence version replaced NM_009172.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046317.1, SRR1660811.82345.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCGCC AGACTGCTAC AGCATTACCC ACTGGCACCT CAAAGTGTCC ACCATCCCAG 
AGGGTACCTG CCTTGACCGG CACAACTGCA TCCAACAATG ACTTGGCGAG TCTTTTTGAG 
TGTCCTGTCT GCTTTGACTA TGTGTTGCCA CCTATTCTTC AGTGTCAGAG TGGCCATCTT 
GTTTGTAGCA ACTGTCGCCC CAAACTTACA TGTTGTCCCA CTTGCCGGGG CCCATTGGGA 
TCCATTCGCA ACTTGGCTAT GGAGAAAGTG GCCAACTCAG TACTCTTCCC TTGTAAATAT 
GCCTCTTCTG GATGTGAAAT AACTCTGCCA CACACCGAAA AGGCAGAGCA CGAGGAGCTC 
TGTGAGTTCA GGCCTTACTC CTGCCCCTGC CCTGGTGCTT CCTGTAAGTG GCAAGGCTCC 
TTGGATGCCG TCATGCCCCA CCTGATGCAT CAGCACAAGT CCATTACCAC CCTGCAAGGA 
GAAGATATAG TTTTCCTTGC TACAGACATT AACCTTCCTG GTGCTGTTGA CTGGGTGATG 
ATGCAGTCTT GTTTTGGCTT TCATTTCATG TTAGTCTTGG AGAAACAAGA AAAATATGAT 
GGTCATCAGC AGTTCTTTGC AATTGTACAA CTGATAGGAA CACGCAAGCA AGCTGAAAAT 
TTTGCATATC GACTTGAGCT AAATGGTCAT AGGCGGCGAT TGACTTGGGA AGCGACTCCT 
CGGTCTATTC ATGAGGGAAT TGCAACAGCC ATTATGAATA GTGACTGCCT AGTGTTTGAC 
ACCAGCATTG CACAGCTTTT TGCAGAAAAT GGCAATTTAG GCATCAATGT AACTATTTCC 
ATGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033198.1
CDS	193..1041
Translation	MSRQTATALPTGTSKCPPSQRVPALTGTTASNNDLASLFECPVCFDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKYASSGCEITLPHTEKAEHEELCEFRPYSCPCPGASCKWQGSLDAVMPHLMHQHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMNSDCLVFDTSIAQLFAENGNLGINVTISMC

Target ORF information:

RefSeq Version	NM_009172.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus siah E3 ubiquitin protein ligase 1A (Siah1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009172.2

ORF Insert Sequence:

ATGAGCCGCC AGACTGCTAC AGCATTACCC ACTGGCACCT CAAAGTGTCC ACCATCCCAG 
AGGGTACCTG CCTTGACCGG CACAACTGCA TCCAACAATG ACTTGGCGAG TCTTTTTGAG 
TGTCCTGTCT GCTTTGACTA TGTGTTGCCA CCTATTCTTC AGTGTCAGAG TGGCCATCTT 
GTTTGTAGCA ACTGTCGCCC CAAACTTACA TGTTGTCCCA CTTGCCGGGG CCCATTGGGA 
TCCATTCGCA ACTTGGCTAT GGAGAAAGTG GCCAACTCAG TACTCTTCCC TTGTAAATAT 
GCCTCTTCTG GATGTGAAAT AACTCTGCCA CACACCGAAA AGGCAGAGCA CGAGGAGCTC 
TGTGAGTTCA GGCCTTACTC CTGCCCCTGC CCTGGTGCTT CCTGTAAGTG GCAAGGCTCC 
TTGGATGCCG TCATGCCCCA CCTGATGCAT CAGCACAAGT CCATTACCAC CCTGCAAGGA 
GAAGATATAG TTTTCCTTGC TACAGACATT AACCTTCCTG GTGCTGTTGA CTGGGTGATG 
ATGCAGTCTT GTTTTGGCTT TCATTTCATG TTAGTCTTGG AGAAACAAGA AAAATATGAT 
GGTCATCAGC AGTTCTTTGC AATTGTACAA CTGATAGGAA CACGCAAGCA AGCTGAAAAT 
TTTGCATATC GACTTGAGCT AAATGGTCAT AGGCGGCGAT TGACTTGGGA AGCGACTCCT 
CGGTCTATTC ATGAGGGAAT TGCAACAGCC ATTATGAATA GTGACTGCCT AGTGTTTGAC 
ACCAGCATTG CACAGCTTTT TGCAGAAAAT GGCAATTTAG GCATCAATGT AACTATTTCC 
ATGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Siah1a ( XM_006530784.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530847.2 Mus musculus seven in absentia 1A (Siah1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03662
Clone ID Related Accession (Same CDS sequence)	XM_006530784.3 , NM_009172.2
Accession Version	XM_006530784.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SIAH1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530784.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCC AGACTGCTAC AGCATTACCC ACTGGCACCT CAAAGTGTCC ACCATCCCAG 
AGGGTACCTG CCTTGACCGG CACAACTGCA TCCAACAATG ACTTGGCGAG TCTTTTTGAG 
TGTCCTGTCT GCTTTGACTA TGTGTTGCCA CCTATTCTTC AGTGTCAGAG TGGCCATCTT 
GTTTGTAGCA ACTGTCGCCC CAAACTTACA TGTTGTCCCA CTTGCCGGGG CCCATTGGGA 
TCCATTCGCA ACTTGGCTAT GGAGAAAGTG GCCAACTCAG TACTCTTCCC TTGTAAATAT 
GCCTCTTCTG GATGTGAAAT AACTCTGCCA CACACCGAAA AGGCAGAGCA CGAGGAGCTC 
TGTGAGTTCA GGCCTTACTC CTGCCCCTGC CCTGGTGCTT CCTGTAAGTG GCAAGGCTCC 
TTGGATGCCG TCATGCCCCA CCTGATGCAT CAGCACAAGT CCATTACCAC CCTGCAAGGA 
GAAGATATAG TTTTCCTTGC TACAGACATT AACCTTCCTG GTGCTGTTGA CTGGGTGATG 
ATGCAGTCTT GTTTTGGCTT TCATTTCATG TTAGTCTTGG AGAAACAAGA AAAATATGAT 
GGTCATCAGC AGTTCTTTGC AATTGTACAA CTGATAGGAA CACGCAAGCA AGCTGAAAAT 
TTTGCATATC GACTTGAGCT AAATGGTCAT AGGCGGCGAT TGACTTGGGA AGCGACTCCT 
CGGTCTATTC ATGAGGGAAT TGCAACAGCC ATTATGAATA GTGACTGCCT AGTGTTTGAC 
ACCAGCATTG CACAGCTTTT TGCAGAAAAT GGCAATTTAG GCATCAATGT AACTATTTCC 
ATGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530847.2
CDS	154..1002
Translation	MSRQTATALPTGTSKCPPSQRVPALTGTTASNNDLASLFECPVCFDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKYASSGCEITLPHTEKAEHEELCEFRPYSCPCPGASCKWQGSLDAVMPHLMHQHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMNSDCLVFDTSIAQLFAENGNLGINVTISMC

Target ORF information:

RefSeq Version	XM_006530784.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus seven in absentia 1A (Siah1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530784.3

ORF Insert Sequence:

ATGAGCCGCC AGACTGCTAC AGCATTACCC ACTGGCACCT CAAAGTGTCC ACCATCCCAG 
AGGGTACCTG CCTTGACCGG CACAACTGCA TCCAACAATG ACTTGGCGAG TCTTTTTGAG 
TGTCCTGTCT GCTTTGACTA TGTGTTGCCA CCTATTCTTC AGTGTCAGAG TGGCCATCTT 
GTTTGTAGCA ACTGTCGCCC CAAACTTACA TGTTGTCCCA CTTGCCGGGG CCCATTGGGA 
TCCATTCGCA ACTTGGCTAT GGAGAAAGTG GCCAACTCAG TACTCTTCCC TTGTAAATAT 
GCCTCTTCTG GATGTGAAAT AACTCTGCCA CACACCGAAA AGGCAGAGCA CGAGGAGCTC 
TGTGAGTTCA GGCCTTACTC CTGCCCCTGC CCTGGTGCTT CCTGTAAGTG GCAAGGCTCC 
TTGGATGCCG TCATGCCCCA CCTGATGCAT CAGCACAAGT CCATTACCAC CCTGCAAGGA 
GAAGATATAG TTTTCCTTGC TACAGACATT AACCTTCCTG GTGCTGTTGA CTGGGTGATG 
ATGCAGTCTT GTTTTGGCTT TCATTTCATG TTAGTCTTGG AGAAACAAGA AAAATATGAT 
GGTCATCAGC AGTTCTTTGC AATTGTACAA CTGATAGGAA CACGCAAGCA AGCTGAAAAT 
TTTGCATATC GACTTGAGCT AAATGGTCAT AGGCGGCGAT TGACTTGGGA AGCGACTCCT 
CGGTCTATTC ATGAGGGAAT TGCAACAGCC ATTATGAATA GTGACTGCCT AGTGTTTGAC 
ACCAGCATTG CACAGCTTTT TGCAGAAAAT GGCAATTTAG GCATCAATGT AACTATTTCC 
ATGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Siah1a ( XM_006530784.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530847.3 Mus musculus siah E3 ubiquitin protein ligase 1A (Siah1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu41299
Clone ID Related Accession (Same CDS sequence)	XM_006530784.4
Accession Version	XM_006530784.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SIAH1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530784.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCAC GAGCGAGCGC GCGGACGCCC GCCTGGGCAG CTCCAGCCTT TAAGCCTAAG 
CCTTGCTCCG CCCAGCTTCC TTTGCGGGAT AGTTCGGGGT CCCTCCAAGC TCCCAGATGC 
AACTTAGTGT GTACGCCCTG GGCCGCTGCG GACCGTCTGT TCCTGGAAAG ATACAGAATT 
TGTTTGCCCT GGCATAAGAG TTACATTTGT GCATTCGAGA TGAGCCGCCA GACTGCTACA 
GCATTACCCA CTGGCACCTC AAAGTGTCCA CCATCCCAGA GGGTACCTGC CTTGACCGGC 
ACAACTGCAT CCAACAATGA CTTGGCGAGT CTTTTTGAGT GTCCTGTCTG CTTTGACTAT 
GTGTTGCCAC CTATTCTTCA GTGTCAGAGT GGCCATCTTG TTTGTAGCAA CTGTCGCCCC 
AAACTTACAT GTTGTCCCAC TTGCCGGGGC CCATTGGGAT CCATTCGCAA CTTGGCTATG 
GAGAAAGTGG CCAACTCAGT ACTCTTCCCT TGTAAATATG CCTCTTCTGG ATGTGAAATA 
ACTCTGCCAC ACACCGAAAA GGCAGAGCAC GAGGAGCTCT GTGAGTTCAG GCCTTACTCC 
TGCCCCTGCC CTGGTGCTTC CTGTAAGTGG CAAGGCTCCT TGGATGCCGT CATGCCCCAC 
CTGATGCATC AGCACAAGTC CATTACCACC CTGCAAGGAG AAGATATAGT TTTCCTTGCT 
ACAGACATTA ACCTTCCTGG TGCTGTTGAC TGGGTGATGA TGCAGTCTTG TTTTGGCTTT 
CATTTCATGT TAGTCTTGGA GAAACAAGAA AAATATGATG GTCATCAGCA GTTCTTTGCA 
ATTGTACAAC TGATAGGAAC ACGCAAGCAA GCTGAAAATT TTGCATATCG ACTTGAGCTA 
AATGGTCATA GGCGGCGATT GACTTGGGAA GCGACTCCTC GGTCTATTCA TGAGGGAATT 
GCAACAGCCA TTATGAATAG TGACTGCCTA GTGTTTGACA CCAGCATTGC ACAGCTTTTT 
GCAGAAAATG GCAATTTAGG CATCAATGTA ACTATTTCCA TGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530847.3
CDS	262..1329
Translation	MSPRASARTPAWAAPAFKPKPCSAQLPLRDSSGSLQAPRCNLVCTPWAAADRLFLERYRICLPWHKSYICAFEMSRQTATALPTGTSKCPPSQRVPALTGTTASNNDLASLFECPVCFDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKYASSGCEITLPHTEKAEHEELCEFRPYSCPCPGASCKWQGSLDAVMPHLMHQHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMNSDCLVFDTSIAQLFAENGNLGINVTISMC

Target ORF information:

RefSeq Version	XM_006530784.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus siah E3 ubiquitin protein ligase 1A (Siah1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530784.4

ORF Insert Sequence:

ATGTCGCCAC GAGCGAGCGC GCGGACGCCC GCCTGGGCAG CTCCAGCCTT TAAGCCTAAG 
CCTTGCTCCG CCCAGCTTCC TTTGCGGGAT AGTTCGGGGT CCCTCCAAGC TCCCAGATGC 
AACTTAGTGT GTACGCCCTG GGCCGCTGCG GACCGTCTGT TCCTGGAAAG ATACAGAATT 
TGTTTGCCCT GGCATAAGAG TTACATTTGT GCATTCGAGA TGAGCCGCCA GACTGCTACA 
GCATTACCCA CTGGCACCTC AAAGTGTCCA CCATCCCAGA GGGTACCTGC CTTGACCGGC 
ACAACTGCAT CCAACAATGA CTTGGCGAGT CTTTTTGAGT GTCCTGTCTG CTTTGACTAT 
GTGTTGCCAC CTATTCTTCA GTGTCAGAGT GGCCATCTTG TTTGTAGCAA CTGTCGCCCC 
AAACTTACAT GTTGTCCCAC TTGCCGGGGC CCATTGGGAT CCATTCGCAA CTTGGCTATG 
GAGAAAGTGG CCAACTCAGT ACTCTTCCCT TGTAAATATG CCTCTTCTGG ATGTGAAATA 
ACTCTGCCAC ACACCGAAAA GGCAGAGCAC GAGGAGCTCT GTGAGTTCAG GCCTTACTCC 
TGCCCCTGCC CTGGTGCTTC CTGTAAGTGG CAAGGCTCCT TGGATGCCGT CATGCCCCAC 
CTGATGCATC AGCACAAGTC CATTACCACC CTGCAAGGAG AAGATATAGT TTTCCTTGCT 
ACAGACATTA ACCTTCCTGG TGCTGTTGAC TGGGTGATGA TGCAGTCTTG TTTTGGCTTT 
CATTTCATGT TAGTCTTGGA GAAACAAGAA AAATATGATG GTCATCAGCA GTTCTTTGCA 
ATTGTACAAC TGATAGGAAC ACGCAAGCAA GCTGAAAATT TTGCATATCG ACTTGAGCTA 
AATGGTCATA GGCGGCGATT GACTTGGGAA GCGACTCCTC GGTCTATTCA TGAGGGAATT 
GCAACAGCCA TTATGAATAG TGACTGCCTA GTGTTTGACA CCAGCATTGC ACAGCTTTTT 
GCAGAAAATG GCAATTTAGG CATCAATGTA ACTATTTCCA TGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HIF-1? coordinates epigenetic activation of SIAH1 in hepatocytes in response to nutritional stress.
Biochimica et biophysica acta1860(10)1037-1046(2017 10)
Fan Z,Li Z,Yang Y,Liu S,Guo J,Xu Y

Central role of SIAH inhibition in DCC-dependent cardioprotection provoked by netrin-1/NO.
Proceedings of the National Academy of Sciences of the United States of America112(3)899-904(2015 Jan)
Li Q,Wang P,Ye K,Cai H

Fine tuning of the UPR by the ubiquitin ligases Siah1/2.
PLoS genetics10(5)e1004348(2014 May)
Scortegagna M,Kim H,Li JL,Yao H,Brill LM,Han J,Lau E,Bowtell D,Haddad G,Kaufman RJ,Ronai ZA

S-nitrosylation of B23/nucleophosmin by GAPDH protects cells from the SIAH1-GAPDH death cascade.
The Journal of cell biology199(1)65-76(2012 Oct)
Lee SB,Kim CK,Lee KH,Ahn JY

miR-135A regulates preimplantation embryo development through down-regulation of E3 Ubiquitin Ligase Seven In Absentia Homolog 1A (SIAH1A) expression.
PloS one6(11)e27878(2011)
Pang RT,Liu WM,Leung CO,Ye TM,Kwan PC,Lee KF,Yeung WS