gRNAs
The following Sh3bp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sh3bp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu03643 | |
| Clone ID Related Accession (Same CDS sequence) | NM_009164.3 | |
| Accession Version | NM_009164.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-29 | |
| Organism | Mus musculus(house mouse) | |
| Product | SH3 domain-binding protein 1 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592169.14. On Oct 22, 2015 this sequence version replaced NM_009164.2. Transcript Variant: This variant (2) uses two alternate splice sites in its 3' coding region, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK166337.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATGAAGA GGCAGTTGCA CCGCATGCGG CAGCTGGCCC ACACGGGCAG CTCGGGACGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_033190.2 |
| CDS | 136..1884 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_009164.3 |
1 | ATGATGAAGA GGCAGTTGCA CCGCATGCGG CAGCTGGCCC ACACGGGCAG CTCGGGACGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu68678 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001316686.1 | |
| Accession Version | NM_001316686.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-05 | |
| Organism | Mus musculus(house mouse) | |
| Product | SH3 domain-binding protein 1 isoform 4 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592169.14 and AK161215.1. On Oct 22, 2015 this sequence version replaced XM_011245527.1. Transcript Variant: This variant (4) has multiple differences compared to variant 1. These differences result in the use of a downstream in-frame start codon and result in an isoform (4) with a shorter N-terminus and a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK161215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCTGAAGG AGGAAGAGGA GGAGCTCAAG AAGAAGGTGG AACAGTGCAA GGACGAGTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001303615.1 |
| CDS | 626..1807 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001316686.1 |
1 | ATGCTGAAGG AGGAAGAGGA GGAGCTCAAG AAGAAGGTGG AACAGTGCAA GGACGAGTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu79072 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017316521.1 | |
| Accession Version | XM_017316521.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | SH3 domain-binding protein 1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGAAGG AGGAAGAGGA GGAGCTCAAG AAGAAGGTGG AACAGTGCAA GGACGAGTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017172010.1 |
| CDS | 621..2096 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017316521.1 |
1 | ATGCTGAAGG AGGAAGAGGA GGAGCTCAAG AAGAAGGTGG AACAGTGCAA GGACGAGTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu51094 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001316684.1 | |
| Accession Version | NM_001316684.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-28 | |
| Organism | Mus musculus(house mouse) | |
| Product | SH3 domain-binding protein 1 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592169.14. On Oct 22, 2015 this sequence version replaced XM_006520661.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATGAAGA GGCAGTTGCA CCGCATGCGG CAGCTGGCCC ACACGGGCAG CTCGGGACGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001303613.1 |
| CDS | 136..2178 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001316684.1 |
1 | ATGATGAAGA GGCAGTTGCA CCGCATGCGG CAGCTGGCCC ACACGGGCAG CTCGGGACGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu83403 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001316685.1 | |
| Accession Version | NM_001316685.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-28 | |
| Organism | Mus musculus(house mouse) | |
| Product | SH3 domain-binding protein 1 isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592169.14. Transcript Variant: This variant (3) uses an alternate splice site in its 3' coding region, compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK172060.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849378, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATGAAGA GGCAGTTGCA CCGCATGCGG CAGCTGGCCC ACACGGGCAG CTCGGGACGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001303614.1 |
| CDS | 136..1890 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001316685.1 |
1 | ATGATGAAGA GGCAGTTGCA CCGCATGCGG CAGCTGGCCC ACACGGGCAG CTCGGGACGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu86671 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030248432.1 | |
| Accession Version | XM_030248432.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | SH3 domain-binding protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGGGA CACTTAGCTC TGGCCATGCA ATGGGGCCAG TGACCACCCA CCTTGGGATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_030104292.1 |
| CDS | 611..2248 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_030248432.1 |
1 | ATGGAGGGGA CACTTAGCTC TGGCCATGCA ATGGGGCCAG TGACCACCCA CCTTGGGATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex. |
3BP-1, an SH3 domain binding protein, has GAP activity for Rac and inhibits growth factor-induced membrane ruffling in fibroblasts. |
Identification of a ten-amino acid proline-rich SH3 binding site. |
Identification of a protein that binds to the SH3 region of Abl and is similar to Bcr and GAP-rho. |
