Sema6a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema6a
Entrez Gene ID	20358
Full Name	sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Synonyms	A730020P05Rik,AI851735,Sema6A-1,Semaq,VIa
General protein information	Preferred Names sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A Names semaphorin-6A Mutant line 997 sema Q sema VIA semaphorin Q semaphorin VIA semaphorin-6A-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525726.1	XP_006525789.1	semaphorin-6A isoform X1
XM_006525727.3	XP_006525790.1	semaphorin-6A isoform X1
XM_006525726.2	XP_006525789.1	semaphorin-6A isoform X1
NM_001311097.1	NP_001298026.1	semaphorin-6A isoform 2 precursor
XM_006525727.4	XP_006525790.1	semaphorin-6A isoform X1
NM_018744.2	NP_061214.2	semaphorin-6A isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	SEMA6A	NP_065847.1
Gallus gallus (chicken)	SEMA6A	XP_003643120.1
Pan troglodytes (chimpanzee)	SEMA6A	XP_001151177.1
	SEMA6A	XP_002804530.1
Canis lupus familiaris (dog)	SEMA6A	XP_858843.1
Mus musculus (house mouse)	Sema6a	NP_061214.2
Xenopus tropicalis (tropical clawed frog)	sema6a	XP_002931763.2
Bos taurus (cattle)	SEMA6A	NP_001180138.1
Danio rerio (zebrafish)	sema6a	NP_956286.1
Rattus norvegicus (Norway rat)	Sema6a	XP_003751830.1

Gene Ontology

The following Sema6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68669	NM_001311097.1 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu22374	NM_018744.2 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu54440	XM_006525726.2 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu54440	XM_006525727.4 Latest version!	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu54440	XM_006525726.1	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu54440	XM_006525727.3	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema6a ( NM_001311097.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001298026.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu68669
Clone ID Related Accession (Same CDS sequence)	NM_001311097.1
Accession Version	NM_001311097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	semaphorin-6A isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK082711.1, AC121783.2, BC062979.1, BC059238.1, AC124466.3, AK034123.1 and AI606937.1. On Jul 22, 2015 this sequence version replaced XM_006525730.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062979.1, SRR1660817.224414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG AGTGATTCGG 
GAAAGTTACC TCAAAAGCAA CGACCAGCTT GTTCCTGTCA CCCTCCTGGC CATTGCAGTC 
ATTCTGGCTT TTGTCATGGG GGCCGTCTTC TCGGGCATCA TCGTGTATTG TGTGTGCGAT 
CACCGGCGCA AAGACGTGGC AGTAGTGCAG CGCAAGGAGA AAGAGCTCAC TCACTCGCGT 
CGGGGATCTA TGAGCAGTGT CACCAAGCTC AGTGGCCTCT TTGGGGACAC CCAGTCCAAG 
GACCCAAAGC CTGAGGCCAT CCTCACACCA CTCATGCACA ACGGCAAGCT GGCCACGCCT 
AGCAACACCG CCAAGATGCT CATCAAGGCT GACCAGCATC ACCTAGACCT CACCGCCCTG 
CCCACCCCAG AGTCCACCCC GACACTGCAG CAGAAACGGA AACCCAACCG CGGCAGTCGC 
GAGTGGGAGA GGAACCAGAA CATCATCAAT GCCTGCACCA AGGACATGCC TCCCATGGGT 
TCCCCTGTGA TTCCCACGGA CCTGCCCCTC CGGGCCTCCC CAAGCCACAT CCCCAGCGTG 
GTGGTCCTGC CCATCACGCA GCAGGGCTAC CAGCACGAGT ACGTAGATCA GCCCAAAATG 
AGCGAGGTGG TGGCTCAGAT GGCACTGGAG GACCAGGCTG CCACCCTGGA GTATAAGACC 
ATCAAAGAGC ACCTGAGTAG CAAGAGTCCC AACCATGGGG TGAACCTTGT GGAGAACCTG 
GACAGCCTGC CCCCTAAAGT TCCACAGCGC GAGGCCTCCC TAGGTCCCCC GGGAACCTCA 
CTGTCACAGA CCGGCCTGAG CAAGAGGCTG GAGATGCAAC ACTCCTCCTC CTATGGGCTC 
GAATATAAGA GGAGCTACCC CACGAACTCG CTCACAAGAA GCCACCAGAC CACCACTCTC 
AAAAGAAACA ATACTAACTC CTCCAATTCC TCCCACCTCT CCAGGAACCA GAGCTTTGGC 
CGGGGAGACA ACCCACCCCC CGCCCCGCAG CGGGTGGACT CTATCCAGGT GCACAGCTCC 
CAGCCCTCTG GCCAGGCCGT GACTGTTTCG AGGCAGCCCA GCCTCAATGC CTACAACTCA 
CTGACGAGGT CGGGGCTGAA GCGCACCCCC TCGCTAAAGC CAGATGTACC CCCCAAACCT 
TCCTTTGCTC CCCTTTCCAC ATCCATGAAG CCCAATGATG CATGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001298026.1
CDS	672..3602
Translation	MRPAALLLCLTLLHCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYVAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNAAGPYQNHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDRASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVRESGSCAHLSPLSRLTFEQDIERGNTDGLGDCHNSFVALNGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDTQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNIINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPPGTSLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQTTTLKRNNTNSSNSSHLSRNQSFGRGDNPPPAPQRVDSIQVHSSQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	NM_001311097.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001311097.1

ORF Insert Sequence:

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG AGTGATTCGG 
GAAAGTTACC TCAAAAGCAA CGACCAGCTT GTTCCTGTCA CCCTCCTGGC CATTGCAGTC 
ATTCTGGCTT TTGTCATGGG GGCCGTCTTC TCGGGCATCA TCGTGTATTG TGTGTGCGAT 
CACCGGCGCA AAGACGTGGC AGTAGTGCAG CGCAAGGAGA AAGAGCTCAC TCACTCGCGT 
CGGGGATCTA TGAGCAGTGT CACCAAGCTC AGTGGCCTCT TTGGGGACAC CCAGTCCAAG 
GACCCAAAGC CTGAGGCCAT CCTCACACCA CTCATGCACA ACGGCAAGCT GGCCACGCCT 
AGCAACACCG CCAAGATGCT CATCAAGGCT GACCAGCATC ACCTAGACCT CACCGCCCTG 
CCCACCCCAG AGTCCACCCC GACACTGCAG CAGAAACGGA AACCCAACCG CGGCAGTCGC 
GAGTGGGAGA GGAACCAGAA CATCATCAAT GCCTGCACCA AGGACATGCC TCCCATGGGT 
TCCCCTGTGA TTCCCACGGA CCTGCCCCTC CGGGCCTCCC CAAGCCACAT CCCCAGCGTG 
GTGGTCCTGC CCATCACGCA GCAGGGCTAC CAGCACGAGT ACGTAGATCA GCCCAAAATG 
AGCGAGGTGG TGGCTCAGAT GGCACTGGAG GACCAGGCTG CCACCCTGGA GTATAAGACC 
ATCAAAGAGC ACCTGAGTAG CAAGAGTCCC AACCATGGGG TGAACCTTGT GGAGAACCTG 
GACAGCCTGC CCCCTAAAGT TCCACAGCGC GAGGCCTCCC TAGGTCCCCC GGGAACCTCA 
CTGTCACAGA CCGGCCTGAG CAAGAGGCTG GAGATGCAAC ACTCCTCCTC CTATGGGCTC 
GAATATAAGA GGAGCTACCC CACGAACTCG CTCACAAGAA GCCACCAGAC CACCACTCTC 
AAAAGAAACA ATACTAACTC CTCCAATTCC TCCCACCTCT CCAGGAACCA GAGCTTTGGC 
CGGGGAGACA ACCCACCCCC CGCCCCGCAG CGGGTGGACT CTATCCAGGT GCACAGCTCC 
CAGCCCTCTG GCCAGGCCGT GACTGTTTCG AGGCAGCCCA GCCTCAATGC CTACAACTCA 
CTGACGAGGT CGGGGCTGAA GCGCACCCCC TCGCTAAAGC CAGATGTACC CCCCAAACCT 
TCCTTTGCTC CCCTTTCCAC ATCCATGAAG CCCAATGATG CATGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( NM_018744.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061214.2 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22374
Clone ID Related Accession (Same CDS sequence)	NM_018744.2
Accession Version	NM_018744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	semaphorin-6A isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK042751.1, BC059238.1, AC124466.3 and AI606937.1. On Jun 16, 2005 this sequence version replaced NM_018744.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.251657.1, BC059238.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG GCACGCCAGT 
TCCCTCTATC CCAGCACCAC TACGTCAGAT TCGGCATCCC GAGACGGGTA TGAGTCTAGG 
GGAGGCATGC TGGACTGGAA CGACCTGCTC GAGGCACCTG GCAGCACAGA CCCTTTGGGG 
GCAGTGTCCT CTCATAACCA CCAGGACAAG AAGGGAGTGA TTCGGGAAAG TTACCTCAAA 
AGCAACGACC AGCTTGTTCC TGTCACCCTC CTGGCCATTG CAGTCATTCT GGCTTTTGTC 
ATGGGGGCCG TCTTCTCGGG CATCATCGTG TATTGTGTGT GCGATCACCG GCGCAAAGAC 
GTGGCAGTAG TGCAGCGCAA GGAGAAAGAG CTCACTCACT CGCGTCGGGG ATCTATGAGC 
AGTGTCACCA AGCTCAGTGG CCTCTTTGGG GACACCCAGT CCAAGGACCC AAAGCCTGAG 
GCCATCCTCA CACCACTCAT GCACAACGGC AAGCTGGCCA CGCCTAGCAA CACCGCCAAG 
ATGCTCATCA AGGCTGACCA GCATCACCTA GACCTCACCG CCCTGCCCAC CCCAGAGTCC 
ACCCCGACAC TGCAGCAGAA ACGGAAACCC AACCGCGGCA GTCGCGAGTG GGAGAGGAAC 
CAGAACATCA TCAATGCCTG CACCAAGGAC ATGCCTCCCA TGGGTTCCCC TGTGATTCCC 
ACGGACCTGC CCCTCCGGGC CTCCCCAAGC CACATCCCCA GCGTGGTGGT CCTGCCCATC 
ACGCAGCAGG GCTACCAGCA CGAGTACGTA GATCAGCCCA AAATGAGCGA GGTGGTGGCT 
CAGATGGCAC TGGAGGACCA GGCTGCCACC CTGGAGTATA AGACCATCAA AGAGCACCTG 
AGTAGCAAGA GTCCCAACCA TGGGGTGAAC CTTGTGGAGA ACCTGGACAG CCTGCCCCCT 
AAAGTTCCAC AGCGCGAGGC CTCCCTAGGT CCCCCGGGAA CCTCACTGTC ACAGACCGGC 
CTGAGCAAGA GGCTGGAGAT GCAACACTCC TCCTCCTATG GGCTCGAATA TAAGAGGAGC 
TACCCCACGA ACTCGCTCAC AAGAAGCCAC CAGACCACCA CTCTCAAAAG AAACAATACT 
AACTCCTCCA ATTCCTCCCA CCTCTCCAGG AACCAGAGCT TTGGCCGGGG AGACAACCCA 
CCCCCCGCCC CGCAGCGGGT GGACTCTATC CAGGTGCACA GCTCCCAGCC CTCTGGCCAG 
GCCGTGACTG TTTCGAGGCA GCCCAGCCTC AATGCCTACA ACTCACTGAC GAGGTCGGGG 
CTGAAGCGCA CCCCCTCGCT AAAGCCAGAT GTACCCCCCA AACCTTCCTT TGCTCCCCTT 
TCCACATCCA TGAAGCCCAA TGATGCATGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061214.2
CDS	670..3765
Translation	MRPAALLLCLTLLHCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYVAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNAAGPYQNHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDRASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVRESGSCAHLSPLSRLTFEQDIERGNTDGLGDCHNSFVALNGHASSLYPSTTTSDSASRDGYESRGGMLDWNDLLEAPGSTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDTQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNIINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPPGTSLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQTTTLKRNNTNSSNSSHLSRNQSFGRGDNPPPAPQRVDSIQVHSSQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	NM_018744.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018744.2

ORF Insert Sequence:

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG GCACGCCAGT 
TCCCTCTATC CCAGCACCAC TACGTCAGAT TCGGCATCCC GAGACGGGTA TGAGTCTAGG 
GGAGGCATGC TGGACTGGAA CGACCTGCTC GAGGCACCTG GCAGCACAGA CCCTTTGGGG 
GCAGTGTCCT CTCATAACCA CCAGGACAAG AAGGGAGTGA TTCGGGAAAG TTACCTCAAA 
AGCAACGACC AGCTTGTTCC TGTCACCCTC CTGGCCATTG CAGTCATTCT GGCTTTTGTC 
ATGGGGGCCG TCTTCTCGGG CATCATCGTG TATTGTGTGT GCGATCACCG GCGCAAAGAC 
GTGGCAGTAG TGCAGCGCAA GGAGAAAGAG CTCACTCACT CGCGTCGGGG ATCTATGAGC 
AGTGTCACCA AGCTCAGTGG CCTCTTTGGG GACACCCAGT CCAAGGACCC AAAGCCTGAG 
GCCATCCTCA CACCACTCAT GCACAACGGC AAGCTGGCCA CGCCTAGCAA CACCGCCAAG 
ATGCTCATCA AGGCTGACCA GCATCACCTA GACCTCACCG CCCTGCCCAC CCCAGAGTCC 
ACCCCGACAC TGCAGCAGAA ACGGAAACCC AACCGCGGCA GTCGCGAGTG GGAGAGGAAC 
CAGAACATCA TCAATGCCTG CACCAAGGAC ATGCCTCCCA TGGGTTCCCC TGTGATTCCC 
ACGGACCTGC CCCTCCGGGC CTCCCCAAGC CACATCCCCA GCGTGGTGGT CCTGCCCATC 
ACGCAGCAGG GCTACCAGCA CGAGTACGTA GATCAGCCCA AAATGAGCGA GGTGGTGGCT 
CAGATGGCAC TGGAGGACCA GGCTGCCACC CTGGAGTATA AGACCATCAA AGAGCACCTG 
AGTAGCAAGA GTCCCAACCA TGGGGTGAAC CTTGTGGAGA ACCTGGACAG CCTGCCCCCT 
AAAGTTCCAC AGCGCGAGGC CTCCCTAGGT CCCCCGGGAA CCTCACTGTC ACAGACCGGC 
CTGAGCAAGA GGCTGGAGAT GCAACACTCC TCCTCCTATG GGCTCGAATA TAAGAGGAGC 
TACCCCACGA ACTCGCTCAC AAGAAGCCAC CAGACCACCA CTCTCAAAAG AAACAATACT 
AACTCCTCCA ATTCCTCCCA CCTCTCCAGG AACCAGAGCT TTGGCCGGGG AGACAACCCA 
CCCCCCGCCC CGCAGCGGGT GGACTCTATC CAGGTGCACA GCTCCCAGCC CTCTGGCCAG 
GCCGTGACTG TTTCGAGGCA GCCCAGCCTC AATGCCTACA ACTCACTGAC GAGGTCGGGG 
CTGAAGCGCA CCCCCTCGCT AAAGCCAGAT GTACCCCCCA AACCTTCCTT TGCTCCCCTT 
TCCACATCCA TGAAGCCCAA TGATGCATGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_006525726.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525789.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54440
Clone ID Related Accession (Same CDS sequence)	XM_006525726.1 , XM_006525727.3 , XM_006525726.2 , XM_006525727.4
Accession Version	XM_006525726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC AGAGTGTATG GGCACGCCAG TTCCCTCTAT 
CCCAGCACCA CTACGTCAGA TTCGGCATCC CGAGACGGGT ATGAGTCTAG GGGAGGCATG 
CTGGACTGGA ACGACCTGCT CGAGGCACCT GGCAGCACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTTGTTC CTGTCACCCT CCTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCC 
GTCTTCTCGG GCATCATCGT GTATTGTGTG TGCGATCACC GGCGCAAAGA CGTGGCAGTA 
GTGCAGCGCA AGGAGAAAGA GCTCACTCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACACCCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAACGG CAAGCTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACCT AGACCTCACC GCCCTGCCCA CCCCAGAGTC CACCCCGACA 
CTGCAGCAGA AACGGAAACC CAACCGCGGC AGTCGCGAGT GGGAGAGGAA CCAGAACATC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGTTCCC CTGTGATTCC CACGGACCTG 
CCCCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACGCAGCAG 
GGCTACCAGC ACGAGTACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGTAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC TAAAGTTCCA 
CAGCGCGAGG CCTCCCTAGG TCCCCCGGGA ACCTCACTGT CACAGACCGG CCTGAGCAAG 
AGGCTGGAGA TGCAACACTC CTCCTCCTAT GGGCTCGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA CCAGACCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC TTTGGCCGGG GAGACAACCC ACCCCCCGCC 
CCGCAGCGGG TGGACTCTAT CCAGGTGCAC AGCTCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCTCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCATG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525789.1
CDS	3002..6148
Translation	MRPAALLLCLTLLHCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYVAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNAAGPYQNHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDRASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVRESGSCAHLSPLSRLTFEQDIERGNTDGLGDCHNSFVALNDISTPLPDYEMSFNRVYGHASSLYPSTTTSDSASRDGYESRGGMLDWNDLLEAPGSTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDTQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNIINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPPGTSLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQTTTLKRNNTNSSNSSHLSRNQSFGRGDNPPPAPQRVDSIQVHSSQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_006525726.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525726.2

ORF Insert Sequence:

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC AGAGTGTATG GGCACGCCAG TTCCCTCTAT 
CCCAGCACCA CTACGTCAGA TTCGGCATCC CGAGACGGGT ATGAGTCTAG GGGAGGCATG 
CTGGACTGGA ACGACCTGCT CGAGGCACCT GGCAGCACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTTGTTC CTGTCACCCT CCTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCC 
GTCTTCTCGG GCATCATCGT GTATTGTGTG TGCGATCACC GGCGCAAAGA CGTGGCAGTA 
GTGCAGCGCA AGGAGAAAGA GCTCACTCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACACCCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAACGG CAAGCTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACCT AGACCTCACC GCCCTGCCCA CCCCAGAGTC CACCCCGACA 
CTGCAGCAGA AACGGAAACC CAACCGCGGC AGTCGCGAGT GGGAGAGGAA CCAGAACATC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGTTCCC CTGTGATTCC CACGGACCTG 
CCCCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACGCAGCAG 
GGCTACCAGC ACGAGTACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGTAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC TAAAGTTCCA 
CAGCGCGAGG CCTCCCTAGG TCCCCCGGGA ACCTCACTGT CACAGACCGG CCTGAGCAAG 
AGGCTGGAGA TGCAACACTC CTCCTCCTAT GGGCTCGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA CCAGACCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC TTTGGCCGGG GAGACAACCC ACCCCCCGCC 
CCGCAGCGGG TGGACTCTAT CCAGGTGCAC AGCTCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCTCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCATG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_006525727.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525790.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54440
Clone ID Related Accession (Same CDS sequence)	XM_006525726.1 , XM_006525727.3 , XM_006525726.2 , XM_006525727.4
Accession Version	XM_006525727.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525727.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC AGAGTGTATG GGCACGCCAG TTCCCTCTAT 
CCCAGCACCA CTACGTCAGA TTCGGCATCC CGAGACGGGT ATGAGTCTAG GGGAGGCATG 
CTGGACTGGA ACGACCTGCT CGAGGCACCT GGCAGCACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTTGTTC CTGTCACCCT CCTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCC 
GTCTTCTCGG GCATCATCGT GTATTGTGTG TGCGATCACC GGCGCAAAGA CGTGGCAGTA 
GTGCAGCGCA AGGAGAAAGA GCTCACTCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACACCCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAACGG CAAGCTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACCT AGACCTCACC GCCCTGCCCA CCCCAGAGTC CACCCCGACA 
CTGCAGCAGA AACGGAAACC CAACCGCGGC AGTCGCGAGT GGGAGAGGAA CCAGAACATC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGTTCCC CTGTGATTCC CACGGACCTG 
CCCCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACGCAGCAG 
GGCTACCAGC ACGAGTACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGTAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC TAAAGTTCCA 
CAGCGCGAGG CCTCCCTAGG TCCCCCGGGA ACCTCACTGT CACAGACCGG CCTGAGCAAG 
AGGCTGGAGA TGCAACACTC CTCCTCCTAT GGGCTCGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA CCAGACCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC TTTGGCCGGG GAGACAACCC ACCCCCCGCC 
CCGCAGCGGG TGGACTCTAT CCAGGTGCAC AGCTCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCTCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCATG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525790.1
CDS	24213..27359
Translation	MRPAALLLCLTLLHCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYVAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNAAGPYQNHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDRASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVRESGSCAHLSPLSRLTFEQDIERGNTDGLGDCHNSFVALNDISTPLPDYEMSFNRVYGHASSLYPSTTTSDSASRDGYESRGGMLDWNDLLEAPGSTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDTQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNIINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPPGTSLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQTTTLKRNNTNSSNSSHLSRNQSFGRGDNPPPAPQRVDSIQVHSSQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_006525727.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525727.4

ORF Insert Sequence:

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC AGAGTGTATG GGCACGCCAG TTCCCTCTAT 
CCCAGCACCA CTACGTCAGA TTCGGCATCC CGAGACGGGT ATGAGTCTAG GGGAGGCATG 
CTGGACTGGA ACGACCTGCT CGAGGCACCT GGCAGCACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTTGTTC CTGTCACCCT CCTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCC 
GTCTTCTCGG GCATCATCGT GTATTGTGTG TGCGATCACC GGCGCAAAGA CGTGGCAGTA 
GTGCAGCGCA AGGAGAAAGA GCTCACTCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACACCCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAACGG CAAGCTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACCT AGACCTCACC GCCCTGCCCA CCCCAGAGTC CACCCCGACA 
CTGCAGCAGA AACGGAAACC CAACCGCGGC AGTCGCGAGT GGGAGAGGAA CCAGAACATC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGTTCCC CTGTGATTCC CACGGACCTG 
CCCCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACGCAGCAG 
GGCTACCAGC ACGAGTACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGTAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC TAAAGTTCCA 
CAGCGCGAGG CCTCCCTAGG TCCCCCGGGA ACCTCACTGT CACAGACCGG CCTGAGCAAG 
AGGCTGGAGA TGCAACACTC CTCCTCCTAT GGGCTCGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA CCAGACCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC TTTGGCCGGG GAGACAACCC ACCCCCCGCC 
CCGCAGCGGG TGGACTCTAT CCAGGTGCAC AGCTCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCTCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCATG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_006525726.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525789.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54440
Clone ID Related Accession (Same CDS sequence)	XM_006525726.1 , XM_006525727.3 , XM_006525726.2 , XM_006525727.4
Accession Version	XM_006525726.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC AGAGTGTATG GGCACGCCAG TTCCCTCTAT 
CCCAGCACCA CTACGTCAGA TTCGGCATCC CGAGACGGGT ATGAGTCTAG GGGAGGCATG 
CTGGACTGGA ACGACCTGCT CGAGGCACCT GGCAGCACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTTGTTC CTGTCACCCT CCTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCC 
GTCTTCTCGG GCATCATCGT GTATTGTGTG TGCGATCACC GGCGCAAAGA CGTGGCAGTA 
GTGCAGCGCA AGGAGAAAGA GCTCACTCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACACCCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAACGG CAAGCTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACCT AGACCTCACC GCCCTGCCCA CCCCAGAGTC CACCCCGACA 
CTGCAGCAGA AACGGAAACC CAACCGCGGC AGTCGCGAGT GGGAGAGGAA CCAGAACATC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGTTCCC CTGTGATTCC CACGGACCTG 
CCCCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACGCAGCAG 
GGCTACCAGC ACGAGTACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGTAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC TAAAGTTCCA 
CAGCGCGAGG CCTCCCTAGG TCCCCCGGGA ACCTCACTGT CACAGACCGG CCTGAGCAAG 
AGGCTGGAGA TGCAACACTC CTCCTCCTAT GGGCTCGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA CCAGACCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC TTTGGCCGGG GAGACAACCC ACCCCCCGCC 
CCGCAGCGGG TGGACTCTAT CCAGGTGCAC AGCTCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCTCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCATG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525789.1
CDS	1015..4161
Translation	MRPAALLLCLTLLHCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYVAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNAAGPYQNHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDRASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVRESGSCAHLSPLSRLTFEQDIERGNTDGLGDCHNSFVALNDISTPLPDYEMSFNRVYGHASSLYPSTTTSDSASRDGYESRGGMLDWNDLLEAPGSTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDTQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNIINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPPGTSLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQTTTLKRNNTNSSNSSHLSRNQSFGRGDNPPPAPQRVDSIQVHSSQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_006525726.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525726.1

ORF Insert Sequence:

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC AGAGTGTATG GGCACGCCAG TTCCCTCTAT 
CCCAGCACCA CTACGTCAGA TTCGGCATCC CGAGACGGGT ATGAGTCTAG GGGAGGCATG 
CTGGACTGGA ACGACCTGCT CGAGGCACCT GGCAGCACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTTGTTC CTGTCACCCT CCTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCC 
GTCTTCTCGG GCATCATCGT GTATTGTGTG TGCGATCACC GGCGCAAAGA CGTGGCAGTA 
GTGCAGCGCA AGGAGAAAGA GCTCACTCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACACCCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAACGG CAAGCTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACCT AGACCTCACC GCCCTGCCCA CCCCAGAGTC CACCCCGACA 
CTGCAGCAGA AACGGAAACC CAACCGCGGC AGTCGCGAGT GGGAGAGGAA CCAGAACATC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGTTCCC CTGTGATTCC CACGGACCTG 
CCCCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACGCAGCAG 
GGCTACCAGC ACGAGTACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGTAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC TAAAGTTCCA 
CAGCGCGAGG CCTCCCTAGG TCCCCCGGGA ACCTCACTGT CACAGACCGG CCTGAGCAAG 
AGGCTGGAGA TGCAACACTC CTCCTCCTAT GGGCTCGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA CCAGACCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC TTTGGCCGGG GAGACAACCC ACCCCCCGCC 
CCGCAGCGGG TGGACTCTAT CCAGGTGCAC AGCTCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCTCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCATG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_006525727.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525790.1 Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54440
Clone ID Related Accession (Same CDS sequence)	XM_006525726.1 , XM_006525727.3 , XM_006525726.2 , XM_006525727.4
Accession Version	XM_006525727.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC AGAGTGTATG GGCACGCCAG TTCCCTCTAT 
CCCAGCACCA CTACGTCAGA TTCGGCATCC CGAGACGGGT ATGAGTCTAG GGGAGGCATG 
CTGGACTGGA ACGACCTGCT CGAGGCACCT GGCAGCACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTTGTTC CTGTCACCCT CCTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCC 
GTCTTCTCGG GCATCATCGT GTATTGTGTG TGCGATCACC GGCGCAAAGA CGTGGCAGTA 
GTGCAGCGCA AGGAGAAAGA GCTCACTCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACACCCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAACGG CAAGCTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACCT AGACCTCACC GCCCTGCCCA CCCCAGAGTC CACCCCGACA 
CTGCAGCAGA AACGGAAACC CAACCGCGGC AGTCGCGAGT GGGAGAGGAA CCAGAACATC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGTTCCC CTGTGATTCC CACGGACCTG 
CCCCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACGCAGCAG 
GGCTACCAGC ACGAGTACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGTAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC TAAAGTTCCA 
CAGCGCGAGG CCTCCCTAGG TCCCCCGGGA ACCTCACTGT CACAGACCGG CCTGAGCAAG 
AGGCTGGAGA TGCAACACTC CTCCTCCTAT GGGCTCGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA CCAGACCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC TTTGGCCGGG GAGACAACCC ACCCCCCGCC 
CCGCAGCGGG TGGACTCTAT CCAGGTGCAC AGCTCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCTCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCATG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525790.1
CDS	323..3469
Translation	MRPAALLLCLTLLHCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYVAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNAAGPYQNHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDRASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVRESGSCAHLSPLSRLTFEQDIERGNTDGLGDCHNSFVALNDISTPLPDYEMSFNRVYGHASSLYPSTTTSDSASRDGYESRGGMLDWNDLLEAPGSTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDTQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNIINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPPGTSLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQTTTLKRNNTNSSNSSHLSRNQSFGRGDNPPPAPQRVDSIQVHSSQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_006525727.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525727.3

ORF Insert Sequence:

ATGCGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC ACTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CCGAGCCAAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACGTT GCTGCTCGAG ACCATATTTA TACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGACG TAGACACATG CAGGATGAAG GGGAAACATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAATGATGAT ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTCGAT ACCTTGGAAA CTTTTGGGGA TGAATTTAGC 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGA 
AAACTCTACT CGGCTACAGT GACTGACTTT CTGGCCATTG ATGCAGTCAT TTACAGGAGC 
CTCGGAGACA GCCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCCGTGGA TTATGGGGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAATACA ACACTATGGG GAAGGTTGTT TTCCCTAGGG TGGCTCAGGT CTGTAAGAAT 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCTTTCCT GAAGGCTCGC 
CTGAACTGCT CGGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GCATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCAG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCTAA TGTTTTCACT 
GGGAGGTTCA AGGAACAGAA ATCACCTGAC TCTACCTGGA CACCCGTTCC AGACGAACGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCCGATGA TACCCTGAAC TTCATTAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATCATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CGCTGCCGGG CCATATCAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CCTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGCATG CAGCTCGACA GAGCGAGTGG CTCACTCTAT 
GTTGCATTCT CTACTTGTGT GATCAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGCATCGC CTCCAGAGAC CCGTATTGTG GGTGGGTAAG GGAAAGTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGACATTTG AGCAGGACAT TGAGCGTGGC 
AATACGGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC AGAGTGTATG GGCACGCCAG TTCCCTCTAT 
CCCAGCACCA CTACGTCAGA TTCGGCATCC CGAGACGGGT ATGAGTCTAG GGGAGGCATG 
CTGGACTGGA ACGACCTGCT CGAGGCACCT GGCAGCACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTTGTTC CTGTCACCCT CCTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCC 
GTCTTCTCGG GCATCATCGT GTATTGTGTG TGCGATCACC GGCGCAAAGA CGTGGCAGTA 
GTGCAGCGCA AGGAGAAAGA GCTCACTCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACACCCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAACGG CAAGCTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACCT AGACCTCACC GCCCTGCCCA CCCCAGAGTC CACCCCGACA 
CTGCAGCAGA AACGGAAACC CAACCGCGGC AGTCGCGAGT GGGAGAGGAA CCAGAACATC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGTTCCC CTGTGATTCC CACGGACCTG 
CCCCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACGCAGCAG 
GGCTACCAGC ACGAGTACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGTAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC TAAAGTTCCA 
CAGCGCGAGG CCTCCCTAGG TCCCCCGGGA ACCTCACTGT CACAGACCGG CCTGAGCAAG 
AGGCTGGAGA TGCAACACTC CTCCTCCTAT GGGCTCGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA CCAGACCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC TTTGGCCGGG GAGACAACCC ACCCCCCGCC 
CCGCAGCGGG TGGACTCTAT CCAGGTGCAC AGCTCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCTCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCATG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Sema6a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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