Sema5a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema5a
Entrez Gene ID	20356
Full Name	sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms	5930434A13,9130201M22Rik,AI464145,Semaf,semF
General protein information	Preferred Names sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A Names semaphorin-5A M-Sema D sema F semaphorin F
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 B3.1\|15 13.02 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006520043.4	XP_006520106.1	semaphorin-5A isoform X1
XM_030248431.1	XP_030104291.1	semaphorin-5A isoform X1
XM_030248430.1	XP_030104290.1	semaphorin-5A isoform X1
XM_011245340.2	XP_011243642.1	semaphorin-5A isoform X1
XM_006520043.3	XP_006520106.1	semaphorin-5A isoform X1
NM_009154.2	NP_033180.2	semaphorin-5A precursor
XM_011245340.1	XP_011243642.1	semaphorin-5A isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SEMA5A	XP_005639731.1
Rattus norvegicus (Norway rat)	Sema5a	NP_001101129.1
Bos taurus (cattle)	SEMA5A	XP_005221710.1
Gallus gallus (chicken)	SEMA5A	XP_419016.3
Danio rerio (zebrafish)	sema5a	NP_001258599.1
Pan troglodytes (chimpanzee)	SEMA5A	XP_517627.2
Mus musculus (house mouse)	Sema5a	NP_033180.2
Homo sapiens (human)	SEMA5A	NP_003957.2
	SEMA5A	XP_002804352.1

Gene Ontology
Bibliography

Related articles in PubMed

PlexinA2 Forward Signaling through Rap1 GTPases Regulates Dentate Gyrus Development and Schizophrenia-like Behaviors.
Zhao XF, Kohen R, Parent R, Duan Y, Fisher GL, Korn MJ, Ji L, Wan G, Jin J, P?schel AW, Dolan DF, Parent JM, Corfas G, Murphy GG, Giger RJ
Cell reports22(2)456-470(2018 Jan)

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Memic F, Knoflach V, Morarach K, Sadler R, Laranjeira C, Hjerling-Leffler J, Sundstr?m E, Pachnis V, Marklund U
Gastroenterology(2017 Oct)

Semaphorin 5A inhibits synaptogenesis in early postnatal- and adult-born hippocampal dentate granule cells.
Duan Y, Wang SH, Song J, Mironova Y, Ming GL, Kolodkin AL, Giger RJ
eLife3(2014 Oct)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Development of the prethalamus is crucial for thalamocortical projection formation and is regulated by Olig2.
Ono K, Clavairoly A, Nomura T, Gotoh H, Uno A, Armant O, Takebayashi H, Zhang Q, Shimamura K, Itohara S, Parras CM, Ikenaka K
Development (Cambridge, England)141(10)2075-84(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mouse Sema5A negatively regulates synaptogenesis in early, developmentally born, hippocampal dentate granule cells.
Title: Semaphorin 5A inhibits synaptogenesis in early postnatal- and adult-born hippocampal dentate granule cells.

The results of this study concluded that the Sema5a mice do not meet the behavioral criteria for a mouse model of autism.
Title: Are Sema5a mutant mice a good model of autism? A behavioral analysis of sensory systems, emotionality and cognition.

Sema5A and Sema5B inhibit retinal neurite outgrowth through PlexinA1 and PlexinA3 receptors both in vitro and in vivo.
Title: Class 5 transmembrane semaphorins control selective Mammalian retinal lamination and function.

Data reveal that Sema5A is a novel proangiogenic molecule, inducing endothelial cell proliferation and migration and inhibiting apoptosis.
Title: Semaphorin 5A promotes angiogenesis by increasing endothelial cell proliferation, migration, and decreasing apoptosis.

A role of Sema5A in the remodeling of the cranial blood vessels was shown.
Title: Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system.

The following Sema5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03619	XM_006520043.4 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu03619	XM_030248430.1 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu03619	XM_030248431.1 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu03619	XM_011245340.2 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu03619	NM_009154.2 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu03619	XM_006520043.3	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu03619	XM_011245340.1	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema5a ( XM_006520043.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520106.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu03619
Clone ID Related Accession (Same CDS sequence)	XM_006520043.4 , XM_030248431.1 , XM_030248430.1 , XM_011245340.2 , XM_006520043.3 , NM_009154.2 , XM_011245340.1
Accession Version	XM_006520043.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520043.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520106.1
CDS	248..3472
Translation	MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFHQTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEFQECNILPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQTCPESWSEWSDWSVCDASGTQVRARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_006520043.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520043.4

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5a ( XM_030248431.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104291.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu03619
Clone ID Related Accession (Same CDS sequence)	XM_006520043.4 , XM_030248431.1 , XM_030248430.1 , XM_011245340.2 , XM_006520043.3 , NM_009154.2 , XM_011245340.1
Accession Version	XM_030248431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104291.1
CDS	233..3457
Translation	MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFHQTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEFQECNILPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQTCPESWSEWSDWSVCDASGTQVRARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_030248431.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248431.1

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5a ( XM_030248430.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104290.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X1, mRNA.

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CloneID	OMu03619
Clone ID Related Accession (Same CDS sequence)	XM_006520043.4 , XM_030248431.1 , XM_030248430.1 , XM_011245340.2 , XM_006520043.3 , NM_009154.2 , XM_011245340.1
Accession Version	XM_030248430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104290.1
CDS	454..3678
Translation	MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFHQTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEFQECNILPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQTCPESWSEWSDWSVCDASGTQVRARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_030248430.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248430.1

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5a ( XM_011245340.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243642.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X2, mRNA.

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CloneID	OMu03619
Clone ID Related Accession (Same CDS sequence)	XM_006520043.4 , XM_030248431.1 , XM_030248430.1 , XM_011245340.2 , XM_006520043.3 , NM_009154.2 , XM_011245340.1
Accession Version	XM_011245340.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243642.1
CDS	335..3559
Translation	MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFHQTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEFQECNILPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQTCPESWSEWSDWSVCDASGTQVRARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_011245340.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245340.2

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5a ( NM_009154.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033180.2 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), mRNA.

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CloneID	OMu03619
Clone ID Related Accession (Same CDS sequence)	XM_006520043.4 , XM_030248431.1 , XM_030248430.1 , XM_011245340.2 , XM_006520043.3 , NM_009154.2 , XM_011245340.1
Accession Version	NM_009154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	semaphorin-5A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB643469.1, AK141612.1 and AC110236.8. On Dec 9, 2006 this sequence version replaced NM_009154.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143026.1, AK141612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033180.2
CDS	659..3883
Translation	MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFHQTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEFQECNILPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQTCPESWSEWSDWSVCDASGTQVRARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	NM_009154.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009154.2

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5a ( XM_006520043.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520106.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu03619
Clone ID Related Accession (Same CDS sequence)	XM_006520043.4 , XM_030248431.1 , XM_030248430.1 , XM_011245340.2 , XM_006520043.3 , NM_009154.2 , XM_011245340.1
Accession Version	XM_006520043.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520106.1
CDS	374..3598
Translation	MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFHQTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEFQECNILPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQTCPESWSEWSDWSVCDASGTQVRARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_006520043.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520043.3

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5a ( XM_011245340.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243642.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03619
Clone ID Related Accession (Same CDS sequence)	XM_006520043.4 , XM_030248431.1 , XM_030248430.1 , XM_011245340.2 , XM_006520043.3 , NM_009154.2 , XM_011245340.1
Accession Version	XM_011245340.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243642.1
CDS	821..4045
Translation	MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFHQTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEFQECNILPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQTCPESWSEWSDWSVCDASGTQVRARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_011245340.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245340.1

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
AGGCCCGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCTGT CGTCTCTTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CGAGAAACTA TCTCTTCAGA 
TTAGAGCTTG AGGATCTGTC TCTCATCCAG GCTGTGGAGT GGGAGTGTGA CGAAGCCACC 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTTG GTGGGGACCG GCTATTCACC TGTGGGACCA ATGCCTTCAC ACCTGTCTGT 
ACCATCCGCT CGTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC 
TGTCCCTACA GTCCCCAGCA CAATTCTACC GCCCTACTCA CTGCCAGTGG TGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGA GATCCAGCCA TTTACCGAAG TCTGGGCACT 
TTGCCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGAAA TTTCACCTAC TTCTTCTTCC GAGAAAACGC CGTGGAACAT 
GACTGTGGGA AGACGGTGTT CTCCAGGGCT GCCCGGGTCT GTAAAAATGA CATTGGAGGG 
CGATTTCTTC TGGAGGACAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCCCGG 
CCTGGTGAGG TGCCCTTCTA CTACAATGAG CTGCAGGGTA CTTTCTTCCT GCCGGAGCTG 
GATCTGATCT ATGGTATCTT CACCACCAAT GTAAACAGCA TCGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAAAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTACGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATT 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCCT CTTTCATGGA GGACAACAGC 
CGCTTCTCCC ACTTAGCTGT TGATGTTGTG CAAGGCAGGG AGACTCTGGT CCACATCATT 
TATCTGGCCA CAGATTATGG CACCATTAAG AAAGTACGAG CTCCCCTGAG TCAGAGCTCA 
GGCAGCTGTT TGCTGGAAGA GATTGAGCTT TTCCCAGAGA GGAGGAGTGA GCCCATCAGG 
AGCCTGCAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GACTACAGGA GCATGTGGCC 
AAGATCCCCC TGAAGAGGTG CCACTTCCAT CAAACACGCA GTGCCTGCAT TGGCGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAATGCA CCAGCCTGGA GGAGAGTCTG 
AGCATGACCC AGTGGGATCA GAGCATCCCC ACCTGTCCGA CCAGAAATCT CACTGTAGAT 
GGGAGCTTTG GCCCATGGTC ACCGTGGACA CCCTGTACAC ACACTGATGG CACTGCTGTG 
GGCTCCTGCC TCTGCCGGTC CCGCTCCTGT GACAGCCCAG CTCCTCAGTG TGGTGGTTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCGTG CAGCACGACC TGTGGCATTG GCTTCCAAGT GCGGCAGCGA 
TCCTGCAGCA ACCCCACGCC CAGGCATGGC GGGCGCGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTGCTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GGTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCCCGCCG GAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCGGAGC TGAAGAAGAC CACACCCTGG ACACCCTGGA CCCCTGTCAA CATCTCTGAC 
AATGGAGGTC ACTATGAGCA GCGTTTCCGC TATACCTGTA AAGCTCGCCT GCCAGATCCA 
AATTTGCTGG AAGTAGGAAG ACAGAGGATA GAAATGCGGT ACTGTTCCAG CGATGGAACC 
AGTGGCTGCT CCACAGACGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCT 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CAGAACCCAA GTTTGGGGGC 
ATGCCATGCC TTGGCCCATC GCTGGAGTTC CAGGAATGCA ACATTTTACC TTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGTT CAGCAACCTG TGGAGGTGGC 
CACTACATGA GAACCCGTTC TTGTTCGAAT CCAGCCCCAG CATATGGAGG GGACATCTGC 
CTGGGACTGC ACACAGAAGA GGCACTCTGC AACACACAGA CCTGCCCAGA AAGCTGGTCA 
GAGTGGTCAG ACTGGTCTGT GTGCGATGCA TCTGGTACCC AGGTCCGTGC TCGGCAGTGC 
ATCCTTCTGT TTCCAGTGGG CAGCCAATGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGCAG CGTAGAAGAA 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCCG TGGGGCTTAG CAGTTCCATT 
CTTGGCTGCC TCCTCACACT GCTTGTCTAC ACCTACTGCC AGAGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA CCCTGTCTCT CCTGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGACAA ATATGATTCT GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PlexinA2 Forward Signaling through Rap1 GTPases Regulates Dentate Gyrus Development and Schizophrenia-like Behaviors.
Cell reports22(2)456-470(2018 Jan)
Zhao XF,Kohen R,Parent R,Duan Y,Fisher GL,Korn MJ,Ji L,Wan G,Jin J,P?schel AW,Dolan DF,Parent JM,Corfas G,Murphy GG,Giger RJ

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Gastroenterology(2017 Oct)
Memic F,Knoflach V,Morarach K,Sadler R,Laranjeira C,Hjerling-Leffler J,Sundstr?m E,Pachnis V,Marklund U

Semaphorin 5A inhibits synaptogenesis in early postnatal- and adult-born hippocampal dentate granule cells.
eLife3(2014 Oct)
Duan Y,Wang SH,Song J,Mironova Y,Ming GL,Kolodkin AL,Giger RJ

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Development of the prethalamus is crucial for thalamocortical projection formation and is regulated by Olig2.
Development (Cambridge, England)141(10)2075-84(2014 May)
Ono K,Clavairoly A,Nomura T,Gotoh H,Uno A,Armant O,Takebayashi H,Zhang Q,Shimamura K,Itohara S,Parras CM,Ikenaka K

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Sema5a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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