Scx cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scx
Entrez Gene ID	20289
Full Name	scleraxis
Synonyms	BB114693,Bhlha41,Scl
General protein information	Preferred Names scleraxis Names basic helix-loop-helix transcription factor scleraxis
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 D3\|15 35.92 cM
Summary	This gene encodes a basic helix-loop-helix type transcription factor involved in mesoderm and heart valve formation. The encoded protein is expressed during embryonic development of tendons and ligaments. The gene product regulates collagen type I gene expression in cardiac fibroblasts and myofibroblasts, and it may play a role in myocardial remodeling. The protein is expressed in the scar area of the adult heart following myocardial infarction. [provided by RefSeq, Feb 2010].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006520660.3	XP_006520723.1	basic helix-loop-helix transcription factor scleraxis isoform X1
NM_198885.3	NP_942588.1	basic helix-loop-helix transcription factor scleraxis
XM_006520660.4	XP_006520723.1	basic helix-loop-helix transcription factor scleraxis isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Scx	NP_942588.1
Gallus gallus (chicken)	SCXB	NP_989584.1
Homo sapiens (human)	SCXB	NP_001073983.1
Canis lupus familiaris (dog)	SCXA	XP_005628354.1
Rattus norvegicus (Norway rat)	Scx	NP_001123980.1
Xenopus tropicalis (tropical clawed frog)	LOC100490170	XP_004919846.1
Bos taurus (cattle)	LOC615157	XP_002692591.1
Danio rerio (zebrafish)	scxa	NP_001076538.1

Gene Ontology
Bibliography

Related articles in PubMed

Scleraxis is required for cell lineage differentiation and extracellular matrix remodeling during murine heart valve formation in vivo.
Levay AK, Peacock JD, Lu Y, Koch M, Hinton RB Jr, Kadler KE, Lincoln J
Circulation research103(9)948-56(2008 Oct)

Generation of transgenic tendon reporters, ScxGFP and ScxAP, using regulatory elements of the scleraxis gene.
Pryce BA, Brent AE, Murchison ND, Tabin CJ, Schweitzer R
Developmental dynamics : an official publication of the American Association of Anatomists236(6)1677-82(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deletion of Scx led to altered long bone properties and callus healing.
Title: Loss of scleraxis in mice leads to geometric and structural changes in cortical bone, as well as asymmetry in fracture healing.

scleraxis was required for TGFbeta-induced fibronectin expression: TGFbeta lost its ability to induce fibronectin expression following scleraxis knockdown.
Title: Regulation of fibronectin gene expression in cardiac fibroblasts by scleraxis.

Cardiac fibroblast phenotype regulation by scleraxis.
Title: The transcription factor scleraxis is a critical regulator of cardiac fibroblast phenotype.

The phosphorylation sequence matched that targeted by Casein Kinase 2, and inhibition of this kinase activity disrupted the ability of scleraxis to modulate the expression of its target genes while also attenuating TGFbeta-induced expression of type I collagen and myofibroblast phenotype conversion marker genes
Title: Regulation of scleraxis transcriptional activity by serine phosphorylation.

deletion of Scx led to impairments in enthesis structure.
Title: Scleraxis is required for the development of a functional tendon enthesis.

The following Scx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu51093	XM_006520660.4 Latest version!	Mus musculus scleraxis (Scx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu19449	NM_198885.3 Latest version!	Mus musculus scleraxis (Scx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OMu51093	XM_006520660.3	Mus musculus scleraxis (Scx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scx ( XM_006520660.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520723.1 Mus musculus scleraxis (Scx), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51093
Clone ID Related Accession (Same CDS sequence)	XM_006520660.3 , XM_006520660.4
Accession Version	XM_006520660.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	basic helix-loop-helix transcription factor scleraxis isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520660.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCG CCATGCTGCG TTCAGCGCCG CCGCCGGGTC GCTACCTGTA CCCTGAGGTG 
AGCCCGCTGT CGGAGGATGA GGACCGCGGA AGCGAGAGCT CGGGCTCCGA CGAGAAACCC 
TGCCGTGTGC ATGCTGCGCG CTGTGGCCTC CAGGGCGCCC GGCGGCGGGC AGGAGGACGG 
AGGGCCGCGG GTAGCGGGCC AGGACCCGGG GGGCGGCCAG GCCGCGAGCC CCGGCAGCGG 
CACACAGCGA ATGCGCGCGA GCGGGACCGC ACCAACAGCG TGAACACGGC CTTCACTGCG 
CTGCGCACAC TCATCCCCAC CGAGCCAGCG GACCGCAAGC TCTCCAAGAT TGAGACGCTG 
CGCCTGGCCT CCAGCTACAT TTCTCACCTG GGCAATGTGC TGCTGGTGGG TGAGGCCTGT 
GGCGACGGGC AACCATGCCA CTCGGGACCC GCTTTCTTCC ACAGCGGTCG TGCGGGCAGC 
CCACTGCCAC CGCCGCCGCC GCCACCACCA CTGGCCAGAG ACGGCGGCGA GAACACCCAG 
CCCAAACAGA TCTGCACCTT CTGCCTCAGC AACCAGAGAA AGTTGCCCAC CTCCACCCCA 
AGGCTTATGG GCACCTGCTG CCTCTCCCCT GGTCTACTGA GACCTGCAGT AGAGGGGCTA 
GGGGAGAAGG CTGAACTGAG AGATGCTGGC CAGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520723.1
CDS	192..890
Translation	MSFAMLRSAPPPGRYLYPEVSPLSEDEDRGSESSGSDEKPCRVHAARCGLQGARRRAGGRRAAGSGPGPGGRPGREPRQRHTANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVLLVGEACGDGQPCHSGPAFFHSGRAGSPLPPPPPPPPLARDGGENTQPKQICTFCLSNQRKLPTSTPRLMGTCCLSPGLLRPAVEGLGEKAELRDAGQP

Target ORF information:

RefSeq Version	XM_006520660.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus scleraxis (Scx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520660.4

ORF Insert Sequence:

ATGTCCTTCG CCATGCTGCG TTCAGCGCCG CCGCCGGGTC GCTACCTGTA CCCTGAGGTG 
AGCCCGCTGT CGGAGGATGA GGACCGCGGA AGCGAGAGCT CGGGCTCCGA CGAGAAACCC 
TGCCGTGTGC ATGCTGCGCG CTGTGGCCTC CAGGGCGCCC GGCGGCGGGC AGGAGGACGG 
AGGGCCGCGG GTAGCGGGCC AGGACCCGGG GGGCGGCCAG GCCGCGAGCC CCGGCAGCGG 
CACACAGCGA ATGCGCGCGA GCGGGACCGC ACCAACAGCG TGAACACGGC CTTCACTGCG 
CTGCGCACAC TCATCCCCAC CGAGCCAGCG GACCGCAAGC TCTCCAAGAT TGAGACGCTG 
CGCCTGGCCT CCAGCTACAT TTCTCACCTG GGCAATGTGC TGCTGGTGGG TGAGGCCTGT 
GGCGACGGGC AACCATGCCA CTCGGGACCC GCTTTCTTCC ACAGCGGTCG TGCGGGCAGC 
CCACTGCCAC CGCCGCCGCC GCCACCACCA CTGGCCAGAG ACGGCGGCGA GAACACCCAG 
CCCAAACAGA TCTGCACCTT CTGCCTCAGC AACCAGAGAA AGTTGCCCAC CTCCACCCCA 
AGGCTTATGG GCACCTGCTG CCTCTCCCCT GGTCTACTGA GACCTGCAGT AGAGGGGCTA 
GGGGAGAAGG CTGAACTGAG AGATGCTGGC CAGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scx ( NM_198885.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_942588.1 Mus musculus scleraxis (Scx), mRNA.

Price & Availability↑

CloneID	OMu19449
Clone ID Related Accession (Same CDS sequence)	NM_198885.3
Accession Version	NM_198885.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	basic helix-loop-helix transcription factor scleraxis
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC157566.6 and AV162294.1. On Feb 18, 2010 this sequence version replaced NM_198885.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062161.1, CB204742.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTTCG CCATGCTGCG TTCAGCGCCG CCGCCGGGTC GCTACCTGTA CCCTGAGGTG 
AGCCCGCTGT CGGAGGATGA GGACCGCGGA AGCGAGAGCT CGGGCTCCGA CGAGAAACCC 
TGCCGTGTGC ATGCTGCGCG CTGTGGCCTC CAGGGCGCCC GGCGGCGGGC AGGAGGACGG 
AGGGCCGCGG GTAGCGGGCC AGGACCCGGG GGGCGGCCAG GCCGCGAGCC CCGGCAGCGG 
CACACAGCGA ATGCGCGCGA GCGGGACCGC ACCAACAGCG TGAACACGGC CTTCACTGCG 
CTGCGCACAC TCATCCCCAC CGAGCCAGCG GACCGCAAGC TCTCCAAGAT TGAGACGCTG 
CGCCTGGCCT CCAGCTACAT TTCTCACCTG GGCAATGTGC TGCTGGTGGG TGAGGCCTGT 
GGCGACGGGC AACCATGCCA CTCGGGACCC GCTTTCTTCC ACAGCGGTCG TGCGGGCAGC 
CCACTGCCAC CGCCGCCGCC GCCACCACCA CTGGCCAGAG ACGGCGGCGA GAACACCCAG 
CCCAAACAGA TCTGCACCTT CTGCCTCAGC AACCAGAGAA AGTTGAGCAA AGACCGTGAC 
AGAAAGACGG CGATTCGAAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942588.1
CDS	165..788
Translation	MSFAMLRSAPPPGRYLYPEVSPLSEDEDRGSESSGSDEKPCRVHAARCGLQGARRRAGGRRAAGSGPGPGGRPGREPRQRHTANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVLLVGEACGDGQPCHSGPAFFHSGRAGSPLPPPPPPPPLARDGGENTQPKQICTFCLSNQRKLSKDRDRKTAIRS

Target ORF information:

RefSeq Version	NM_198885.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus scleraxis (Scx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198885.3

ORF Insert Sequence:

ATGTCCTTCG CCATGCTGCG TTCAGCGCCG CCGCCGGGTC GCTACCTGTA CCCTGAGGTG 
AGCCCGCTGT CGGAGGATGA GGACCGCGGA AGCGAGAGCT CGGGCTCCGA CGAGAAACCC 
TGCCGTGTGC ATGCTGCGCG CTGTGGCCTC CAGGGCGCCC GGCGGCGGGC AGGAGGACGG 
AGGGCCGCGG GTAGCGGGCC AGGACCCGGG GGGCGGCCAG GCCGCGAGCC CCGGCAGCGG 
CACACAGCGA ATGCGCGCGA GCGGGACCGC ACCAACAGCG TGAACACGGC CTTCACTGCG 
CTGCGCACAC TCATCCCCAC CGAGCCAGCG GACCGCAAGC TCTCCAAGAT TGAGACGCTG 
CGCCTGGCCT CCAGCTACAT TTCTCACCTG GGCAATGTGC TGCTGGTGGG TGAGGCCTGT 
GGCGACGGGC AACCATGCCA CTCGGGACCC GCTTTCTTCC ACAGCGGTCG TGCGGGCAGC 
CCACTGCCAC CGCCGCCGCC GCCACCACCA CTGGCCAGAG ACGGCGGCGA GAACACCCAG 
CCCAAACAGA TCTGCACCTT CTGCCTCAGC AACCAGAGAA AGTTGAGCAA AGACCGTGAC 
AGAAAGACGG CGATTCGAAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scx ( XM_006520660.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520723.1 Mus musculus scleraxis (Scx), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51093
Clone ID Related Accession (Same CDS sequence)	XM_006520660.3 , XM_006520660.4
Accession Version	XM_006520660.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	basic helix-loop-helix transcription factor scleraxis isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520660.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCG CCATGCTGCG TTCAGCGCCG CCGCCGGGTC GCTACCTGTA CCCTGAGGTG 
AGCCCGCTGT CGGAGGATGA GGACCGCGGA AGCGAGAGCT CGGGCTCCGA CGAGAAACCC 
TGCCGTGTGC ATGCTGCGCG CTGTGGCCTC CAGGGCGCCC GGCGGCGGGC AGGAGGACGG 
AGGGCCGCGG GTAGCGGGCC AGGACCCGGG GGGCGGCCAG GCCGCGAGCC CCGGCAGCGG 
CACACAGCGA ATGCGCGCGA GCGGGACCGC ACCAACAGCG TGAACACGGC CTTCACTGCG 
CTGCGCACAC TCATCCCCAC CGAGCCAGCG GACCGCAAGC TCTCCAAGAT TGAGACGCTG 
CGCCTGGCCT CCAGCTACAT TTCTCACCTG GGCAATGTGC TGCTGGTGGG TGAGGCCTGT 
GGCGACGGGC AACCATGCCA CTCGGGACCC GCTTTCTTCC ACAGCGGTCG TGCGGGCAGC 
CCACTGCCAC CGCCGCCGCC GCCACCACCA CTGGCCAGAG ACGGCGGCGA GAACACCCAG 
CCCAAACAGA TCTGCACCTT CTGCCTCAGC AACCAGAGAA AGTTGCCCAC CTCCACCCCA 
AGGCTTATGG GCACCTGCTG CCTCTCCCCT GGTCTACTGA GACCTGCAGT AGAGGGGCTA 
GGGGAGAAGG CTGAACTGAG AGATGCTGGC CAGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520723.1
CDS	511..1209
Translation	MSFAMLRSAPPPGRYLYPEVSPLSEDEDRGSESSGSDEKPCRVHAARCGLQGARRRAGGRRAAGSGPGPGGRPGREPRQRHTANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVLLVGEACGDGQPCHSGPAFFHSGRAGSPLPPPPPPPPLARDGGENTQPKQICTFCLSNQRKLPTSTPRLMGTCCLSPGLLRPAVEGLGEKAELRDAGQP

Target ORF information:

RefSeq Version	XM_006520660.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus scleraxis (Scx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520660.3

ORF Insert Sequence:

ATGTCCTTCG CCATGCTGCG TTCAGCGCCG CCGCCGGGTC GCTACCTGTA CCCTGAGGTG 
AGCCCGCTGT CGGAGGATGA GGACCGCGGA AGCGAGAGCT CGGGCTCCGA CGAGAAACCC 
TGCCGTGTGC ATGCTGCGCG CTGTGGCCTC CAGGGCGCCC GGCGGCGGGC AGGAGGACGG 
AGGGCCGCGG GTAGCGGGCC AGGACCCGGG GGGCGGCCAG GCCGCGAGCC CCGGCAGCGG 
CACACAGCGA ATGCGCGCGA GCGGGACCGC ACCAACAGCG TGAACACGGC CTTCACTGCG 
CTGCGCACAC TCATCCCCAC CGAGCCAGCG GACCGCAAGC TCTCCAAGAT TGAGACGCTG 
CGCCTGGCCT CCAGCTACAT TTCTCACCTG GGCAATGTGC TGCTGGTGGG TGAGGCCTGT 
GGCGACGGGC AACCATGCCA CTCGGGACCC GCTTTCTTCC ACAGCGGTCG TGCGGGCAGC 
CCACTGCCAC CGCCGCCGCC GCCACCACCA CTGGCCAGAG ACGGCGGCGA GAACACCCAG 
CCCAAACAGA TCTGCACCTT CTGCCTCAGC AACCAGAGAA AGTTGCCCAC CTCCACCCCA 
AGGCTTATGG GCACCTGCTG CCTCTCCCCT GGTCTACTGA GACCTGCAGT AGAGGGGCTA 
GGGGAGAAGG CTGAACTGAG AGATGCTGGC CAGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Scleraxis is required for cell lineage differentiation and extracellular matrix remodeling during murine heart valve formation in vivo.
Circulation research103(9)948-56(2008 Oct)
Levay AK,Peacock JD,Lu Y,Koch M,Hinton RB Jr,Kadler KE,Lincoln J

Generation of transgenic tendon reporters, ScxGFP and ScxAP, using regulatory elements of the scleraxis gene.
Developmental dynamics : an official publication of the American Association of Anatomists236(6)1677-82(2007 Jun)
Pryce BA,Brent AE,Murchison ND,Tabin CJ,Schweitzer R

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Scx cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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