Polr2j cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr2j
Entrez Gene ID	20022
Full Name	polymerase (RNA) II (DNA directed) polypeptide J
Synonyms	14.5kDa,Polr2i,Rpb11a,Rpo2-4
General protein information	Preferred Names polymerase (RNA) II (DNA directed) polypeptide J Names DNA-directed RNA polymerase II subunit RPB11 DNA-directed RNA polymerase II 13.3 kDa polypeptide DNA-directed RNA polymerase II subunit J RNA polymerase II 13.3 kDa subunit RNA polymerase II 4 (14 kDa subunit) RNA polymerase II subunit B11 RNA polymerase II subunit RPB14 RPB14 polymerase (RNA) II (DNA directed) polypeptide I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
NM_011293.2	NP_035423.2	DNA-directed RNA polymerase II subunit RPB11

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC100686835	XP_003434782.2
Xenopus tropicalis (tropical clawed frog)	polr2j	NP_001039095.1
Pan troglodytes (chimpanzee)	POLR2J	XP_003949275.1
Drosophila melanogaster (fruit fly)	Rpb11	NP_609836.1
Bos taurus (cattle)	POLR2J	NP_001032560.1
Rattus norvegicus (Norway rat)	Polr2j	NP_001099391.1
Danio rerio (zebrafish)	polr2j	NP_001019571.1
Arabidopsis thaliana (thale cress)	NRPB11	NP_190777.1
Oryza sativa (rice)	Os07g0172500	NP_001058998.1
Caenorhabditis elegans (roundworm)	rpb-11	NP_496942.1
Homo sapiens (human)	POLR2J	NP_006225.1
Mus musculus (house mouse)	Polr2j	NP_035423.2
Anopheles gambiae (African malaria mosquito)	RPB11_ANOGA	XP_319985.2

Gene Ontology
Bibliography

Related articles in PubMed

Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins.
Zhang M, Chen D, Xia J, Han W, Cui X, Neuenkirchen N, Hermes G, Sestan N, Lin H
Genes & development(2017 Aug)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

RNA polymerase II subunit 3 is retained in the cytoplasm by its interaction with HCR, the psoriasis vulgaris candidate gene product.
Corbi N, Bruno T, De Angelis R, Di Padova M, Libri V, Di Certo MG, Spinardi L, Floridi A, Fanciulli M, Passananti C
Journal of cell science118(Pt 18)4253-60(2005 Sep)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.
Yamamoto K, Yamamoto M, Hanada K, Nogi Y, Matsuyama T, Muramatsu M
Molecular and cellular biology24(14)6338-49(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polr2j gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr2j cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07846	NM_011293.2 Latest version!	Mus musculus polymerase (RNA) II (DNA directed) polypeptide J (Polr2j), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr2j ( NM_011293.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035423.2 Mus musculus polymerase (RNA) II (DNA directed) polypeptide J (Polr2j), mRNA.

Price & Availability↑

CloneID	OMu07846
Clone ID Related Accession (Same CDS sequence)	NM_011293.2
Accession Version	NM_011293.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase II subunit RPB11
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158610.1 and DV065741.1. On May 15, 2008 this sequence version replaced NM_011293.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK158610.1, CN836797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACGCTC CTCCGGCCTT CGAGTCGTTC TTGCTCTTCG AGGGCGAGAA GAAAATCACC 
ATTAACAAGG ACACTAAGGT TCCCAACGCC TGCTTGTTCA CCATCAACAA AGAAGACCAC 
ACTCTGGGGA ACATCATTAA ATCGCAGCTG TTGAAGGACC CGCAGGTGCT GTTTGCTGGC 
TACAAAGTCC CTCACCCCTT GGAGCACAAG ATCATCATTC GTGTGCAGAC CACCCCAGAC 
TACAGTCCCC AGGAGGCTTT CACCAACGCC ATCACAGACC TCATCAGCGA GCTCTCCCTT 
CTGGAGGAGC GATTCCGGGT GGCCATCAAG GACAAGCAAG AAGGAATTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035423.2
CDS	63..416
Translation	MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAIKDKQEGIE

Target ORF information:

RefSeq Version	NM_011293.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) II (DNA directed) polypeptide J (Polr2j), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011293.2

ORF Insert Sequence:

ATGAACGCTC CTCCGGCCTT CGAGTCGTTC TTGCTCTTCG AGGGCGAGAA GAAAATCACC 
ATTAACAAGG ACACTAAGGT TCCCAACGCC TGCTTGTTCA CCATCAACAA AGAAGACCAC 
ACTCTGGGGA ACATCATTAA ATCGCAGCTG TTGAAGGACC CGCAGGTGCT GTTTGCTGGC 
TACAAAGTCC CTCACCCCTT GGAGCACAAG ATCATCATTC GTGTGCAGAC CACCCCAGAC 
TACAGTCCCC AGGAGGCTTT CACCAACGCC ATCACAGACC TCATCAGCGA GCTCTCCCTT 
CTGGAGGAGC GATTCCGGGT GGCCATCAAG GACAAGCAAG AAGGAATTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins.
Genes & development(2017 Aug)
Zhang M,Chen D,Xia J,Han W,Cui X,Neuenkirchen N,Hermes G,Sestan N,Lin H

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

RNA polymerase II subunit 3 is retained in the cytoplasm by its interaction with HCR, the psoriasis vulgaris candidate gene product.
Journal of cell science118(Pt 18)4253-60(2005 Sep)
Corbi N,Bruno T,De Angelis R,Di Padova M,Libri V,Di Certo MG,Spinardi L,Floridi A,Fanciulli M,Passananti C

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.
Molecular and cellular biology24(14)6338-49(2004 Jul)
Yamamoto K,Yamamoto M,Hanada K,Nogi Y,Matsuyama T,Muramatsu M