Rnf7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf7
Entrez Gene ID	19823
Full Name	ring finger protein 7
Synonyms	Rbx2,SAG
General protein information	Preferred Names ring finger protein 7 Names RING-box protein 2 sensitive to apoptosis gene protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001311135.1	NP_001298064.1	RING-box protein 2 isoform 2
NM_011279.3	NP_035409.1	RING-box protein 2 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rnf7	NP_001100318.1
Gallus gallus (chicken)	RNF7	NP_001026478.1
Mus musculus (house mouse)	Rnf7	NP_035409.1
Homo sapiens (human)	RNF7	NP_055060.1
	RNF7	XP_001112690.1
Caenorhabditis elegans (roundworm)	rbx-2	NP_491849.1
Pan troglodytes (chimpanzee)	RNF7	NP_001239486.1
Drosophila melanogaster (fruit fly)	Roc2	NP_001163119.1
Bos taurus (cattle)	LOC515595	NP_001069188.1
Danio rerio (zebrafish)	rnf7	NP_001012516.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005562	XP_315566.3
Xenopus tropicalis (tropical clawed frog)	rnf7	NP_001107733.1

Gene Ontology
Bibliography

Related articles in PubMed

SAG/Rbx2-Dependent Neddylation Regulates T-Cell Responses.
Mathewson ND, Fujiwara H, Wu SR, Toubai T, Oravecz-Wilson K, Sun Y, Rossi C, Zajac C, Sun Y, Reddy P
The American journal of pathology186(10)2679-91(2016 Oct)

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
Wu Y, Wei FY, Kawarada L, Suzuki T, Araki K, Komohara Y, Fujimura A, Kaitsuka T, Takeya M, Oike Y, Suzuki T, Tomizawa K
PLoS genetics12(9)e1006355(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SAG is a novel molecular target that regulates T-cell responses and that inhibiting neddylation with the clinically available small-molecule MLN4924 may represent a novel strategy to mitigate T-cell-mediated immunopathologies, such as graft-versus-host disease.
Title: SAG/Rbx2-Dependent Neddylation Regulates T-Cell Responses.

as a novel substrate of SAG-betaTrCP E3 ligase. By degrading Erbin and Nrf2, Sag activates the Ras-Raf pathway and causes ROS accumulation to trigger autophagy and senescence
Title: Erbin is a novel substrate of the Sag-?TrCP E3 ligase that regulates KrasG12D-induced skin tumorigenesis.

Inactivation of Sag/Rbx2/Roc2 e3 ubiquitin ligase triggers senescence and inhibits kras-induced immortalization.
Title: Inactivation of Sag/Rbx2/Roc2 e3 ubiquitin ligase triggers senescence and inhibits kras-induced immortalization.

Attenuation of SAG expression, exacerbates 4-hydroxy-2-nonenal-induced apoptosis and hypertrophy via a disruption of the cellular redox balance.
Title: Attenuated SAG expression exacerbates 4-hydroxy-2-nonenal-induced apoptosis and hypertrophy of H9c2 cardiomyocytes.

Sag is a Kras-cooperating oncogene that promotes lung tumorigenesis
Title: Inactivation of SAG/RBX2 E3 ubiquitin ligase suppresses KrasG12D-driven lung tumorigenesis.

The following Rnf7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17860	NM_011279.3 Latest version!	Mus musculus ring finger protein 7 (Rnf7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu68622	NM_001311135.1 Latest version!	Mus musculus ring finger protein 7 (Rnf7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf7 ( NM_011279.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035409.1 Mus musculus ring finger protein 7 (Rnf7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17860
Clone ID Related Accession (Same CDS sequence)	NM_011279.3
Accession Version	NM_011279.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	RING-box protein 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK006703.1, AK003248.1, AK011328.1, BF018220.1 and AC161257.2. On Jul 24, 2015 this sequence version replaced NM_011279.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF092877.1, AK146030.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849381 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGACG TGGAGGACGG CGAGGAACCC TGCGTCCTTT CTTCGCACTC CGGGAGCGCA 
GGCTCCAAGT CGGGAGGCGA CAAGATGTTC TCTCTCAAGA AGTGGAACGC GGTAGCCATG 
TGGAGCTGGG ACGTTGAGTG CGATACCTGT GCCATCTGCA GGGTCCAGGT GATGGATGCC 
TGCCTTCGAT GTCAAGCTGA AAACAAGCAA GAGGACTGTG TTGTGGTCTG GGGAGAGTGT 
AACCATTCCT TCCACAACTG CTGCATGTCC CTGTGGGTGA AACAGAACAA TCGCTGCCCT 
CTGTGCCAGC AGGACTGGGT AGTCCAAAGA ATCGGCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035409.1
CDS	94..435
Translation	MADVEDGEEPCVLSSHSGSAGSKSGGDKMFSLKKWNAVAMWSWDVECDTCAICRVQVMDACLRCQAENKQEDCVVVWGECNHSFHNCCMSLWVKQNNRCPLCQQDWVVQRIGK

Target ORF information:

RefSeq Version	NM_011279.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 7 (Rnf7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011279.3

ORF Insert Sequence:

ATGGCCGACG TGGAGGACGG CGAGGAACCC TGCGTCCTTT CTTCGCACTC CGGGAGCGCA 
GGCTCCAAGT CGGGAGGCGA CAAGATGTTC TCTCTCAAGA AGTGGAACGC GGTAGCCATG 
TGGAGCTGGG ACGTTGAGTG CGATACCTGT GCCATCTGCA GGGTCCAGGT GATGGATGCC 
TGCCTTCGAT GTCAAGCTGA AAACAAGCAA GAGGACTGTG TTGTGGTCTG GGGAGAGTGT 
AACCATTCCT TCCACAACTG CTGCATGTCC CTGTGGGTGA AACAGAACAA TCGCTGCCCT 
CTGTGCCAGC AGGACTGGGT AGTCCAAAGA ATCGGCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf7 ( NM_001311135.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001298064.1 Mus musculus ring finger protein 7 (Rnf7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu68622
Clone ID Related Accession (Same CDS sequence)	NM_001311135.1
Accession Version	NM_001311135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	RING-box protein 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK006703.1, BY781065.1, AK003248.1, AK011328.1, BF018220.1 and AC161257.2. On Jul 24, 2015 this sequence version replaced XM_006510876.2. Transcript Variant: This variant (2) uses an alternate splice site in the central coding region that results in a frame-shift compared to variant 1. It encodes isoform 2, which is shorter than and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY324536.1, BY781065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGACG TGGAGGACGG CGAGGAACCC TGCGTCCTTT CTTCGCACTC CGGGAGCGCA 
GGCTCCAAGT CGGGAGGCGA CAAGATGTTC TCTCTCAAGA AGTGGAACGC GGTAGCCATG 
TGGAGCTGGG ACGTTGAGTG CGATACCTGT GCCATCTGCA GGGTCCAGAT GCCTGCCTTC 
GATGTCAAGC TGAAAACAAG CAAGAGGACT GTGTTGTGGT CTGGGGAGAG TGTAACCATT 
CCTTCCACAA CTGCTGCATG TCCCTGTGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001298064.1
CDS	94..366
Translation	MADVEDGEEPCVLSSHSGSAGSKSGGDKMFSLKKWNAVAMWSWDVECDTCAICRVQMPAFDVKLKTSKRTVLWSGESVTIPSTTAACPCG

Target ORF information:

RefSeq Version	NM_001311135.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 7 (Rnf7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001311135.1

ORF Insert Sequence:

ATGGCCGACG TGGAGGACGG CGAGGAACCC TGCGTCCTTT CTTCGCACTC CGGGAGCGCA 
GGCTCCAAGT CGGGAGGCGA CAAGATGTTC TCTCTCAAGA AGTGGAACGC GGTAGCCATG 
TGGAGCTGGG ACGTTGAGTG CGATACCTGT GCCATCTGCA GGGTCCAGAT GCCTGCCTTC 
GATGTCAAGC TGAAAACAAG CAAGAGGACT GTGTTGTGGT CTGGGGAGAG TGTAACCATT 
CCTTCCACAA CTGCTGCATG TCCCTGTGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SAG/Rbx2-Dependent Neddylation Regulates T-Cell Responses.
The American journal of pathology186(10)2679-91(2016 Oct)
Mathewson ND,Fujiwara H,Wu SR,Toubai T,Oravecz-Wilson K,Sun Y,Rossi C,Zajac C,Sun Y,Reddy P

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
PLoS genetics12(9)e1006355(2016 Sep)
Wu Y,Wei FY,Kawarada L,Suzuki T,Araki K,Komohara Y,Fujimura A,Kaitsuka T,Takeya M,Oike Y,Suzuki T,Tomizawa K

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Rnf7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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