Rfc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rfc1
Entrez Gene ID	19687
Full Name	replication factor C (activator 1) 1
Synonyms	140kDa,Alp145,RFC140,Recc1
General protein information	Preferred Names replication factor C (activator 1) 1 Names replication factor C subunit 1 A1 140 kDa subunit A1-P145 ISRE-binding protein RF-C 140 kDa subunit activator 1 140 kDa subunit activator 1 large subunit activator 1 subunit 1 differentiation-specific element-binding protein replication factor C 1 replication factor C 140 kDa subunit replication factor C large subunit replication factor C, 140 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 C3.1\|5 33.61 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006503801.3	XP_006503864.1	replication factor C subunit 1 isoform X2
NM_001347357.1	NP_001334286.1	replication factor C subunit 1 isoform 1
NM_001347358.1	NP_001334287.1	replication factor C subunit 1 isoform 3
NM_011258.2	NP_035388.2	replication factor C subunit 1 isoform 2
XM_006503801.4	XP_006503864.1	replication factor C subunit 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rfc1	NP_445999.1
Xenopus tropicalis (tropical clawed frog)	rfc1	NP_001186496.1
Pan troglodytes (chimpanzee)	RFC1	XP_001140765.1
Saccharomyces cerevisiae (baker's yeast)	RFC1	NP_014860.1
Kluyveromyces lactis	KLLA0D06897g	XP_453368.1
Neurospora crassa	NCU06767	XP_963539.2
	RFC1	XP_001091287.1
Canis lupus familiaris (dog)	RFC1	XP_005618678.1
Gallus gallus (chicken)	RFC1	NP_001006456.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001290	XP_321857.5
Schizosaccharomyces pombe (fission yeast)	rfc1	NP_596607.1
Mus musculus (house mouse)	Rfc1	NP_035388.2
Eremothecium gossypii	AGOS_ACR102W	NP_983504.2
Homo sapiens (human)	RFC1	NP_001191676.1
Bos taurus (cattle)	RFC1	XP_003586244.2
Danio rerio (zebrafish)	rfc1	NP_001082960.2
Drosophila melanogaster (fruit fly)	Gnf1	NP_001014605.1
Magnaporthe oryzae (rice blast fungus)	MGG_03204	XP_003716779.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Gene expression profiling identifies eleven DNA repair genes down-regulated during mouse neural crest cell migration.
Albino D, Brizzolara A, Moretti S, Falugi C, Mirisola V, Scaruffi P, Di Candia M, Truini M, Coco S, Bonassi S, Tonini GP
The International journal of developmental biology55(1)65-72(2011)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Genomic analysis of mouse retinal development.
Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho SH, Yung R, Asch E, Ohno-Machado L, Wong WH, Cepko CL
PLoS biology2(9)E247(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rfc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rfc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu34983	XM_006503801.4 Latest version!	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu07760	NM_011258.2 Latest version!	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$664.30 $949.00
OMu34982	NM_001347357.1 Latest version!	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu34984	NM_001347358.1 Latest version!	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu34983	XM_006503801.3	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rfc1 ( XM_006503801.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503864.1 Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34983
Clone ID Related Accession (Same CDS sequence)	XM_006503801.3 , XM_006503801.4
Accession Version	XM_006503801.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	replication factor C subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503801.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAA AGTTTGGCAA ACTTGCCAGC 
AAAGATGATG GCTCCAGTTT CAAGGCAGCT CTGCTGTCTG GCCCTCCAGG TGTTGGCAAA 
ACCACTACAG CTTCTCTTGT GTGTCAGGAA TTGGGCTACA GTTATGTGGA ACTAAATGCA 
AGTGATACTC GGAGTAAGAA CAGTTTGAAA GCAGTTGTTG CTGAATCCTT GAACAACACC 
AGCATCAAAG GCTTTTATAC AAGTGGAGCG GCTCCTTCCG TGAGTGCGAG GCACGCCCTC 
ATCATGGATG AGGTCGATGG CATGGCAGGC AATGAAGACA GGGGTGGAAT TCAGGAGCTA 
ATAGGCCTGA TAAAGCATAC TAAAATTCCC ATCATTTGTA TGTGCAATGA TAGAAACCAC 
CCCAAAATTC GCTCATTGGT TCATTATTGT TTTGATCTTC GTTTCCAAAG ACCTCGAGTT 
GAACAGATTA AGAGTGCAAT GCTGTCTATT GCATTTAAAG AGGGTTTAAA AATCCCCCCT 
CCAGCTATGA ATGAAATAAT TTTGGGAGCT AATCAAGATG TCAGACAGGT TTTACACAAT 
CTCAGTATGT GGTGTGCACA AAGTAAGGCC CTGACCTATG ACCAAGCCAA GGCTGATTCT 
CAGAGGGCTA AGAAGGATAT CAGACTGGGC CCATTTGATG TCACTCGGAA AGTGTTTGCA 
GCTGGAGAGG AGACTGCACA CATGTCACTG ATGGACAAGT CAGATCTCTT CTTCCATGAC 
TATTCCATAG CACCCCTCTT TGTCCAGGAG AACTACCTGC ATGTAAAGCC TGTGGCTGCA 
GGAGGTGACA TGAAAAAGCA CTTGATGCTT TTAAGCCGGG CAGCAGATAG CATATGTGAC 
GGTGACTTAG TGGACAATCA GATCCGGAGT AAGCAAAACT GGAGTCTTCT GCCCACCCAG 
GCCATCTATG CCAGTGTTCT TCCTGGAGAG TTGATGCGGG GCTACATGAC TCAGTTTCCG 
AGCTTCCCGA GCTGGCTGGG GAAGCACTCG TCTACGGGCA AACATGATCG GATTGTTCAG 
GACCTATCCT TGCACATGAG TCTTAGAACT TACTCCAGCA AAAGGACAGT GAACATGGAT 
TACCTGTCAC ACATCAGAGA CGCACTTGTA CGACCCTTGA CCTCACAAGG GGTAGAAGGG 
GCTCAGCATG TCATCAAACT TATGGACACA TACTATCTGA TGAAAGAAGA TTTTGAGAAC 
ATCATGGAAG TTAGCAGCTG GGGGGGCAAG CCCAGTGCCT TTTCCAAGCT GGATCCCAAG 
GTGAAAGCGG CCTTCACAAG AGCCTACAAT AAGGAGGCCC ACCTGACCCC ATACTCACTT 
CAGGTAGTAA AGACATCGAG GCTCAGCACG GGTCCAGCAC TGGATTCTGA GTACAGTGAG 
GAGTTCCAGG AAGATGACAC TCAGTCTGAG AAAGAGCAGG ATGCAGTGGA GACTGACGCC 
ATGATCAAGC AGAAAAAGAC AAGATCTTCA AAACCTTCAA AGTCAGAAAG AGAGAAGGAG 
TCCAAAAAAG GAAAAGGAAA GAATTGGAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503864.1
CDS	3092..6487
Translation	MDIRKFFGVISSGKKPVNETVKNEKTKASEGTVKGKKGVKEAKVNNSGKEDASKPKQHSKKKRIINDSDSESEETVQVKNAKKKSEKLSLSYKPGKVSQKDPVTYVSETDEDDDFVCKKAASKSKENGVSTNSYLGTSNVKKNEENVKTKNKPLSPIKLTPTSVLDYFGTESVQRSGKKMVTSKRKESSQNTEDSRLNDEAIAKQLQLDEDAELERQLHEDEEFARTLALLDEEPKIKKARKDSEEGEESFSSVQDDLSKAEKQKSPNKAELFSTARKTYSPAKHGKGRASEDAKQPCKSAHRKEACSSPKASAKLALMKAKEESSYNETELLAARRKESATEPKGEKTTPKKTKVSPTKRESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLTFVITGVLESIERDEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKILDEDGLLDLIRTMPGKRSKYEMAAEAEMKKEKSKLERTPQKNDQGKRKISPAKKESESKKCKLTLLKNSPMKAVKKEASTCPRGLDVKETHGNRSSNKEECLLWVDKYKPASLKNIIGQQGDQSCANKLLRWLRNWHKSSPEEKKHAKFGKLASKDDGSSFKAALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKNSLKAVVAESLNNTSIKGFYTSGAAPSVSARHALIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDLRFQRPRVEQIKSAMLSIAFKEGLKIPPPAMNEIILGANQDVRQVLHNLSMWCAQSKALTYDQAKADSQRAKKDIRLGPFDVTRKVFAAGEETAHMSLMDKSDLFFHDYSIAPLFVQENYLHVKPVAAGGDMKKHLMLLSRAADSICDGDLVDNQIRSKQNWSLLPTQAIYASVLPGELMRGYMTQFPSFPSWLGKHSSTGKHDRIVQDLSLHMSLRTYSSKRTVNMDYLSHIRDALVRPLTSQGVEGAQHVIKLMDTYYLMKEDFENIMEVSSWGGKPSAFSKLDPKVKAAFTRAYNKEAHLTPYSLQVVKTSRLSTGPALDSEYSEEFQEDDTQSEKEQDAVETDAMIKQKKTRSSKPSKSEREKESKKGKGKNWKK

Target ORF information:

RefSeq Version	XM_006503801.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503801.4

ORF Insert Sequence:

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAA AGTTTGGCAA ACTTGCCAGC 
AAAGATGATG GCTCCAGTTT CAAGGCAGCT CTGCTGTCTG GCCCTCCAGG TGTTGGCAAA 
ACCACTACAG CTTCTCTTGT GTGTCAGGAA TTGGGCTACA GTTATGTGGA ACTAAATGCA 
AGTGATACTC GGAGTAAGAA CAGTTTGAAA GCAGTTGTTG CTGAATCCTT GAACAACACC 
AGCATCAAAG GCTTTTATAC AAGTGGAGCG GCTCCTTCCG TGAGTGCGAG GCACGCCCTC 
ATCATGGATG AGGTCGATGG CATGGCAGGC AATGAAGACA GGGGTGGAAT TCAGGAGCTA 
ATAGGCCTGA TAAAGCATAC TAAAATTCCC ATCATTTGTA TGTGCAATGA TAGAAACCAC 
CCCAAAATTC GCTCATTGGT TCATTATTGT TTTGATCTTC GTTTCCAAAG ACCTCGAGTT 
GAACAGATTA AGAGTGCAAT GCTGTCTATT GCATTTAAAG AGGGTTTAAA AATCCCCCCT 
CCAGCTATGA ATGAAATAAT TTTGGGAGCT AATCAAGATG TCAGACAGGT TTTACACAAT 
CTCAGTATGT GGTGTGCACA AAGTAAGGCC CTGACCTATG ACCAAGCCAA GGCTGATTCT 
CAGAGGGCTA AGAAGGATAT CAGACTGGGC CCATTTGATG TCACTCGGAA AGTGTTTGCA 
GCTGGAGAGG AGACTGCACA CATGTCACTG ATGGACAAGT CAGATCTCTT CTTCCATGAC 
TATTCCATAG CACCCCTCTT TGTCCAGGAG AACTACCTGC ATGTAAAGCC TGTGGCTGCA 
GGAGGTGACA TGAAAAAGCA CTTGATGCTT TTAAGCCGGG CAGCAGATAG CATATGTGAC 
GGTGACTTAG TGGACAATCA GATCCGGAGT AAGCAAAACT GGAGTCTTCT GCCCACCCAG 
GCCATCTATG CCAGTGTTCT TCCTGGAGAG TTGATGCGGG GCTACATGAC TCAGTTTCCG 
AGCTTCCCGA GCTGGCTGGG GAAGCACTCG TCTACGGGCA AACATGATCG GATTGTTCAG 
GACCTATCCT TGCACATGAG TCTTAGAACT TACTCCAGCA AAAGGACAGT GAACATGGAT 
TACCTGTCAC ACATCAGAGA CGCACTTGTA CGACCCTTGA CCTCACAAGG GGTAGAAGGG 
GCTCAGCATG TCATCAAACT TATGGACACA TACTATCTGA TGAAAGAAGA TTTTGAGAAC 
ATCATGGAAG TTAGCAGCTG GGGGGGCAAG CCCAGTGCCT TTTCCAAGCT GGATCCCAAG 
GTGAAAGCGG CCTTCACAAG AGCCTACAAT AAGGAGGCCC ACCTGACCCC ATACTCACTT 
CAGGTAGTAA AGACATCGAG GCTCAGCACG GGTCCAGCAC TGGATTCTGA GTACAGTGAG 
GAGTTCCAGG AAGATGACAC TCAGTCTGAG AAAGAGCAGG ATGCAGTGGA GACTGACGCC 
ATGATCAAGC AGAAAAAGAC AAGATCTTCA AAACCTTCAA AGTCAGAAAG AGAGAAGGAG 
TCCAAAAAAG GAAAAGGAAA GAATTGGAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rfc1 ( NM_011258.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035388.2 Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07760
Clone ID Related Accession (Same CDS sequence)	NM_011258.2
Accession Version	NM_011258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	replication factor C subunit 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY714128.1, BC085174.1, X72711.1 and BG695244.1. On May 15, 2008 this sequence version replaced NM_011258.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X72711.1, U01222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAG CAAAGTTTGG CAAACTTGCC 
AGCAAAGATG ATGGCTCCAG TTTCAAGGCA GCTCTGCTGT CTGGCCCTCC AGGTGTTGGC 
AAAACCACTA CAGCTTCTCT TGTGTGTCAG GAATTGGGCT ACAGTTATGT GGAACTAAAT 
GCAAGTGATA CTCGGAGTAA GAACAGTTTG AAAGCAGTTG TTGCTGAATC CTTGAACAAC 
ACCAGCATCA AAGGCTTTTA TACAAGTGGA GCGGCTCCTT CCGTGAGTGC GAGGCACGCC 
CTCATCATGG ATGAGGTCGA TGGCATGGCA GGCAATGAAG ACAGGGGTGG AATTCAGGAG 
CTAATAGGCC TGATAAAGCA TACTAAAATT CCCATCATTT GTATGTGCAA TGATAGAAAC 
CACCCCAAAA TTCGCTCATT GGTTCATTAT TGTTTTGATC TTCGTTTCCA AAGACCTCGA 
GTTGAACAGA TTAAGAGTGC AATGCTGTCT ATTGCATTTA AAGAGGGTTT AAAAATCCCC 
CCTCCAGCTA TGAATGAAAT AATTTTGGGA GCTAATCAAG ATGTCAGACA GGTTTTACAC 
AATCTCAGTA TGTGGTGTGC ACAAAGTAAG GCCCTGACCT ATGACCAAGC CAAGGCTGAT 
TCTCAGAGGG CTAAGAAGGA TATCAGACTG GGCCCATTTG ATGTCACTCG GAAAGTGTTT 
GCAGCTGGAG AGGAGACTGC ACACATGTCA CTGATGGACA AGTCAGATCT CTTCTTCCAT 
GACTATTCCA TAGCACCCCT CTTTGTCCAG GAGAACTACC TGCATGTAAA GCCTGTGGCT 
GCAGGAGGTG ACATGAAAAA GCACTTGATG CTTTTAAGCC GGGCAGCAGA TAGCATATGT 
GACGGTGACT TAGTGGACAA TCAGATCCGG AGTAAGCAAA ACTGGAGTCT TCTGCCCACC 
CAGGCCATCT ATGCCAGTGT TCTTCCTGGA GAGTTGATGC GGGGCTACAT GACTCAGTTT 
CCGAGCTTCC CGAGCTGGCT GGGGAAGCAC TCGTCTACGG GCAAACATGA TCGGATTGTT 
CAGGACCTAT CCTTGCACAT GAGTCTTAGA ACTTACTCCA GCAAAAGGAC AGTGAACATG 
GATTACCTGT CACACATCAG AGACGCACTT GTACGACCCT TGACCTCACA AGGGGTAGAA 
GGGGCTCAGC ATGTCATCAA ACTTATGGAC ACATACTATC TGATGAAAGA AGATTTTGAG 
AACATCATGG AAGTTAGCAG CTGGGGGGGC AAGCCCAGTG CCTTTTCCAA GCTGGATCCC 
AAGGTGAAAG CGGCCTTCAC AAGAGCCTAC AATAAGGAGG CCCACCTGAC CCCATACTCA 
CTTCAGGTAG TAAAGACATC GAGGCTCAGC ACGGGTCCAG CACTGGATTC TGAGTACAGT 
GAGGAGTTCC AGGAAGATGA CACTCAGTCT GAGAAAGAGC AGGATGCAGT GGAGACTGAC 
GCCATGATCA AGAAAAAGAC AAGATCTTCA AAACCTTCAA AGTCAGAAAG AGAGAAGGAG 
TCCAAAAAAG GAAAAGGAAA GAATTGGAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035388.2
CDS	114..3509
Translation	MDIRKFFGVISSGKKPVNETVKNEKTKASEGTVKGKKGVKEAKVNNSGKEDASKPKQHSKKKRIINDSDSESEETVQVKNAKKKSEKLSLSYKPGKVSQKDPVTYVSETDEDDDFVCKKAASKSKENGVSTNSYLGTSNVKKNEENVKTKNKPLSPIKLTPTSVLDYFGTESVQRSGKKMVTSKRKESSQNTEDSRLNDEAIAKQLQLDEDAELERQLHEDEEFARTLALLDEEPKIKKARKDSEEGEESFSSVQDDLSKAEKQKSPNKAELFSTARKTYSPAKHGKGRASEDAKQPCKSAHRKEACSSPKASAKLALMKAKEESSYNETELLAARRKESATEPKGEKTTPKKTKVSPTKRESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLTFVITGVLESIERDEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKILDEDGLLDLIRTMPGKRSKYEMAAEAEMKKEKSKLERTPQKNDQGKRKISPAKKESESKKCKLTLLKNSPMKAVKKEASTCPRGLDVKETHGNRSSNKEECLLWVDKYKPASLKNIIGQQGDQSCANKLLRWLRNWHKSSPEEKKHAAKFGKLASKDDGSSFKAALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKNSLKAVVAESLNNTSIKGFYTSGAAPSVSARHALIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDLRFQRPRVEQIKSAMLSIAFKEGLKIPPPAMNEIILGANQDVRQVLHNLSMWCAQSKALTYDQAKADSQRAKKDIRLGPFDVTRKVFAAGEETAHMSLMDKSDLFFHDYSIAPLFVQENYLHVKPVAAGGDMKKHLMLLSRAADSICDGDLVDNQIRSKQNWSLLPTQAIYASVLPGELMRGYMTQFPSFPSWLGKHSSTGKHDRIVQDLSLHMSLRTYSSKRTVNMDYLSHIRDALVRPLTSQGVEGAQHVIKLMDTYYLMKEDFENIMEVSSWGGKPSAFSKLDPKVKAAFTRAYNKEAHLTPYSLQVVKTSRLSTGPALDSEYSEEFQEDDTQSEKEQDAVETDAMIKKKTRSSKPSKSEREKESKKGKGKNWKK

Target ORF information:

RefSeq Version	NM_011258.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011258.2

ORF Insert Sequence:

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAG CAAAGTTTGG CAAACTTGCC 
AGCAAAGATG ATGGCTCCAG TTTCAAGGCA GCTCTGCTGT CTGGCCCTCC AGGTGTTGGC 
AAAACCACTA CAGCTTCTCT TGTGTGTCAG GAATTGGGCT ACAGTTATGT GGAACTAAAT 
GCAAGTGATA CTCGGAGTAA GAACAGTTTG AAAGCAGTTG TTGCTGAATC CTTGAACAAC 
ACCAGCATCA AAGGCTTTTA TACAAGTGGA GCGGCTCCTT CCGTGAGTGC GAGGCACGCC 
CTCATCATGG ATGAGGTCGA TGGCATGGCA GGCAATGAAG ACAGGGGTGG AATTCAGGAG 
CTAATAGGCC TGATAAAGCA TACTAAAATT CCCATCATTT GTATGTGCAA TGATAGAAAC 
CACCCCAAAA TTCGCTCATT GGTTCATTAT TGTTTTGATC TTCGTTTCCA AAGACCTCGA 
GTTGAACAGA TTAAGAGTGC AATGCTGTCT ATTGCATTTA AAGAGGGTTT AAAAATCCCC 
CCTCCAGCTA TGAATGAAAT AATTTTGGGA GCTAATCAAG ATGTCAGACA GGTTTTACAC 
AATCTCAGTA TGTGGTGTGC ACAAAGTAAG GCCCTGACCT ATGACCAAGC CAAGGCTGAT 
TCTCAGAGGG CTAAGAAGGA TATCAGACTG GGCCCATTTG ATGTCACTCG GAAAGTGTTT 
GCAGCTGGAG AGGAGACTGC ACACATGTCA CTGATGGACA AGTCAGATCT CTTCTTCCAT 
GACTATTCCA TAGCACCCCT CTTTGTCCAG GAGAACTACC TGCATGTAAA GCCTGTGGCT 
GCAGGAGGTG ACATGAAAAA GCACTTGATG CTTTTAAGCC GGGCAGCAGA TAGCATATGT 
GACGGTGACT TAGTGGACAA TCAGATCCGG AGTAAGCAAA ACTGGAGTCT TCTGCCCACC 
CAGGCCATCT ATGCCAGTGT TCTTCCTGGA GAGTTGATGC GGGGCTACAT GACTCAGTTT 
CCGAGCTTCC CGAGCTGGCT GGGGAAGCAC TCGTCTACGG GCAAACATGA TCGGATTGTT 
CAGGACCTAT CCTTGCACAT GAGTCTTAGA ACTTACTCCA GCAAAAGGAC AGTGAACATG 
GATTACCTGT CACACATCAG AGACGCACTT GTACGACCCT TGACCTCACA AGGGGTAGAA 
GGGGCTCAGC ATGTCATCAA ACTTATGGAC ACATACTATC TGATGAAAGA AGATTTTGAG 
AACATCATGG AAGTTAGCAG CTGGGGGGGC AAGCCCAGTG CCTTTTCCAA GCTGGATCCC 
AAGGTGAAAG CGGCCTTCAC AAGAGCCTAC AATAAGGAGG CCCACCTGAC CCCATACTCA 
CTTCAGGTAG TAAAGACATC GAGGCTCAGC ACGGGTCCAG CACTGGATTC TGAGTACAGT 
GAGGAGTTCC AGGAAGATGA CACTCAGTCT GAGAAAGAGC AGGATGCAGT GGAGACTGAC 
GCCATGATCA AGAAAAAGAC AAGATCTTCA AAACCTTCAA AGTCAGAAAG AGAGAAGGAG 
TCCAAAAAAG GAAAAGGAAA GAATTGGAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rfc1 ( NM_001347357.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334286.1 Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant 1, mRNA.

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CloneID	OMu34982
Clone ID Related Accession (Same CDS sequence)	NM_001347357.1
Accession Version	NM_001347357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	replication factor C subunit 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC138654.4. On Nov 22, 2016 this sequence version replaced XM_006503800.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085173.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAG CAAAGTTTGG CAAACTTGCC 
AGCAAAGATG ATGGCTCCAG TTTCAAGGCA GCTCTGCTGT CTGGCCCTCC AGGTGTTGGC 
AAAACCACTA CAGCTTCTCT TGTGTGTCAG GAATTGGGCT ACAGTTATGT GGAACTAAAT 
GCAAGTGATA CTCGGAGTAA GAACAGTTTG AAAGCAGTTG TTGCTGAATC CTTGAACAAC 
ACCAGCATCA AAGGCTTTTA TACAAGTGGA GCGGCTCCTT CCGTGAGTGC GAGGCACGCC 
CTCATCATGG ATGAGGTCGA TGGCATGGCA GGCAATGAAG ACAGGGGTGG AATTCAGGAG 
CTAATAGGCC TGATAAAGCA TACTAAAATT CCCATCATTT GTATGTGCAA TGATAGAAAC 
CACCCCAAAA TTCGCTCATT GGTTCATTAT TGTTTTGATC TTCGTTTCCA AAGACCTCGA 
GTTGAACAGA TTAAGAGTGC AATGCTGTCT ATTGCATTTA AAGAGGGTTT AAAAATCCCC 
CCTCCAGCTA TGAATGAAAT AATTTTGGGA GCTAATCAAG ATGTCAGACA GGTTTTACAC 
AATCTCAGTA TGTGGTGTGC ACAAAGTAAG GCCCTGACCT ATGACCAAGC CAAGGCTGAT 
TCTCAGAGGG CTAAGAAGGA TATCAGACTG GGCCCATTTG ATGTCACTCG GAAAGTGTTT 
GCAGCTGGAG AGGAGACTGC ACACATGTCA CTGATGGACA AGTCAGATCT CTTCTTCCAT 
GACTATTCCA TAGCACCCCT CTTTGTCCAG GAGAACTACC TGCATGTAAA GCCTGTGGCT 
GCAGGAGGTG ACATGAAAAA GCACTTGATG CTTTTAAGCC GGGCAGCAGA TAGCATATGT 
GACGGTGACT TAGTGGACAA TCAGATCCGG AGTAAGCAAA ACTGGAGTCT TCTGCCCACC 
CAGGCCATCT ATGCCAGTGT TCTTCCTGGA GAGTTGATGC GGGGCTACAT GACTCAGTTT 
CCGAGCTTCC CGAGCTGGCT GGGGAAGCAC TCGTCTACGG GCAAACATGA TCGGATTGTT 
CAGGACCTAT CCTTGCACAT GAGTCTTAGA ACTTACTCCA GCAAAAGGAC AGTGAACATG 
GATTACCTGT CACACATCAG AGACGCACTT GTACGACCCT TGACCTCACA AGGGGTAGAA 
GGGGCTCAGC ATGTCATCAA ACTTATGGAC ACATACTATC TGATGAAAGA AGATTTTGAG 
AACATCATGG AAGTTAGCAG CTGGGGGGGC AAGCCCAGTG CCTTTTCCAA GCTGGATCCC 
AAGGTGAAAG CGGCCTTCAC AAGAGCCTAC AATAAGGAGG CCCACCTGAC CCCATACTCA 
CTTCAGGTAG TAAAGACATC GAGGCTCAGC ACGGGTCCAG CACTGGATTC TGAGTACAGT 
GAGGAGTTCC AGGAAGATGA CACTCAGTCT GAGAAAGAGC AGGATGCAGT GGAGACTGAC 
GCCATGATCA AGCAGAAAAA GACAAGATCT TCAAAACCTT CAAAGTCAGA AAGAGAGAAG 
GAGTCCAAAA AAGGAAAAGG AAAGAATTGG AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334286.1
CDS	114..3512
Translation	MDIRKFFGVISSGKKPVNETVKNEKTKASEGTVKGKKGVKEAKVNNSGKEDASKPKQHSKKKRIINDSDSESEETVQVKNAKKKSEKLSLSYKPGKVSQKDPVTYVSETDEDDDFVCKKAASKSKENGVSTNSYLGTSNVKKNEENVKTKNKPLSPIKLTPTSVLDYFGTESVQRSGKKMVTSKRKESSQNTEDSRLNDEAIAKQLQLDEDAELERQLHEDEEFARTLALLDEEPKIKKARKDSEEGEESFSSVQDDLSKAEKQKSPNKAELFSTARKTYSPAKHGKGRASEDAKQPCKSAHRKEACSSPKASAKLALMKAKEESSYNETELLAARRKESATEPKGEKTTPKKTKVSPTKRESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLTFVITGVLESIERDEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKILDEDGLLDLIRTMPGKRSKYEMAAEAEMKKEKSKLERTPQKNDQGKRKISPAKKESESKKCKLTLLKNSPMKAVKKEASTCPRGLDVKETHGNRSSNKEECLLWVDKYKPASLKNIIGQQGDQSCANKLLRWLRNWHKSSPEEKKHAAKFGKLASKDDGSSFKAALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKNSLKAVVAESLNNTSIKGFYTSGAAPSVSARHALIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDLRFQRPRVEQIKSAMLSIAFKEGLKIPPPAMNEIILGANQDVRQVLHNLSMWCAQSKALTYDQAKADSQRAKKDIRLGPFDVTRKVFAAGEETAHMSLMDKSDLFFHDYSIAPLFVQENYLHVKPVAAGGDMKKHLMLLSRAADSICDGDLVDNQIRSKQNWSLLPTQAIYASVLPGELMRGYMTQFPSFPSWLGKHSSTGKHDRIVQDLSLHMSLRTYSSKRTVNMDYLSHIRDALVRPLTSQGVEGAQHVIKLMDTYYLMKEDFENIMEVSSWGGKPSAFSKLDPKVKAAFTRAYNKEAHLTPYSLQVVKTSRLSTGPALDSEYSEEFQEDDTQSEKEQDAVETDAMIKQKKTRSSKPSKSEREKESKKGKGKNWKK

Target ORF information:

RefSeq Version	NM_001347357.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347357.1

ORF Insert Sequence:

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAG CAAAGTTTGG CAAACTTGCC 
AGCAAAGATG ATGGCTCCAG TTTCAAGGCA GCTCTGCTGT CTGGCCCTCC AGGTGTTGGC 
AAAACCACTA CAGCTTCTCT TGTGTGTCAG GAATTGGGCT ACAGTTATGT GGAACTAAAT 
GCAAGTGATA CTCGGAGTAA GAACAGTTTG AAAGCAGTTG TTGCTGAATC CTTGAACAAC 
ACCAGCATCA AAGGCTTTTA TACAAGTGGA GCGGCTCCTT CCGTGAGTGC GAGGCACGCC 
CTCATCATGG ATGAGGTCGA TGGCATGGCA GGCAATGAAG ACAGGGGTGG AATTCAGGAG 
CTAATAGGCC TGATAAAGCA TACTAAAATT CCCATCATTT GTATGTGCAA TGATAGAAAC 
CACCCCAAAA TTCGCTCATT GGTTCATTAT TGTTTTGATC TTCGTTTCCA AAGACCTCGA 
GTTGAACAGA TTAAGAGTGC AATGCTGTCT ATTGCATTTA AAGAGGGTTT AAAAATCCCC 
CCTCCAGCTA TGAATGAAAT AATTTTGGGA GCTAATCAAG ATGTCAGACA GGTTTTACAC 
AATCTCAGTA TGTGGTGTGC ACAAAGTAAG GCCCTGACCT ATGACCAAGC CAAGGCTGAT 
TCTCAGAGGG CTAAGAAGGA TATCAGACTG GGCCCATTTG ATGTCACTCG GAAAGTGTTT 
GCAGCTGGAG AGGAGACTGC ACACATGTCA CTGATGGACA AGTCAGATCT CTTCTTCCAT 
GACTATTCCA TAGCACCCCT CTTTGTCCAG GAGAACTACC TGCATGTAAA GCCTGTGGCT 
GCAGGAGGTG ACATGAAAAA GCACTTGATG CTTTTAAGCC GGGCAGCAGA TAGCATATGT 
GACGGTGACT TAGTGGACAA TCAGATCCGG AGTAAGCAAA ACTGGAGTCT TCTGCCCACC 
CAGGCCATCT ATGCCAGTGT TCTTCCTGGA GAGTTGATGC GGGGCTACAT GACTCAGTTT 
CCGAGCTTCC CGAGCTGGCT GGGGAAGCAC TCGTCTACGG GCAAACATGA TCGGATTGTT 
CAGGACCTAT CCTTGCACAT GAGTCTTAGA ACTTACTCCA GCAAAAGGAC AGTGAACATG 
GATTACCTGT CACACATCAG AGACGCACTT GTACGACCCT TGACCTCACA AGGGGTAGAA 
GGGGCTCAGC ATGTCATCAA ACTTATGGAC ACATACTATC TGATGAAAGA AGATTTTGAG 
AACATCATGG AAGTTAGCAG CTGGGGGGGC AAGCCCAGTG CCTTTTCCAA GCTGGATCCC 
AAGGTGAAAG CGGCCTTCAC AAGAGCCTAC AATAAGGAGG CCCACCTGAC CCCATACTCA 
CTTCAGGTAG TAAAGACATC GAGGCTCAGC ACGGGTCCAG CACTGGATTC TGAGTACAGT 
GAGGAGTTCC AGGAAGATGA CACTCAGTCT GAGAAAGAGC AGGATGCAGT GGAGACTGAC 
GCCATGATCA AGCAGAAAAA GACAAGATCT TCAAAACCTT CAAAGTCAGA AAGAGAGAAG 
GAGTCCAAAA AAGGAAAAGG AAAGAATTGG AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rfc1 ( NM_001347358.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334287.1 Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant 3, mRNA.

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CloneID	OMu34984
Clone ID Related Accession (Same CDS sequence)	NM_001347358.1
Accession Version	NM_001347358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	replication factor C subunit 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC138654.4. On Nov 22, 2016 this sequence version replaced XM_006503802.3. Transcript Variant: This variant (3) uses two alternate in-frame splice junctions compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U36441.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAA AGTTTGGCAA ACTTGCCAGC 
AAAGATGATG GCTCCAGTTT CAAGGCAGCT CTGCTGTCTG GCCCTCCAGG TGTTGGCAAA 
ACCACTACAG CTTCTCTTGT GTGTCAGGAA TTGGGCTACA GTTATGTGGA ACTAAATGCA 
AGTGATACTC GGAGTAAGAA CAGTTTGAAA GCAGTTGTTG CTGAATCCTT GAACAACACC 
AGCATCAAAG GCTTTTATAC AAGTGGAGCG GCTCCTTCCG TGAGTGCGAG GCACGCCCTC 
ATCATGGATG AGGTCGATGG CATGGCAGGC AATGAAGACA GGGGTGGAAT TCAGGAGCTA 
ATAGGCCTGA TAAAGCATAC TAAAATTCCC ATCATTTGTA TGTGCAATGA TAGAAACCAC 
CCCAAAATTC GCTCATTGGT TCATTATTGT TTTGATCTTC GTTTCCAAAG ACCTCGAGTT 
GAACAGATTA AGAGTGCAAT GCTGTCTATT GCATTTAAAG AGGGTTTAAA AATCCCCCCT 
CCAGCTATGA ATGAAATAAT TTTGGGAGCT AATCAAGATG TCAGACAGGT TTTACACAAT 
CTCAGTATGT GGTGTGCACA AAGTAAGGCC CTGACCTATG ACCAAGCCAA GGCTGATTCT 
CAGAGGGCTA AGAAGGATAT CAGACTGGGC CCATTTGATG TCACTCGGAA AGTGTTTGCA 
GCTGGAGAGG AGACTGCACA CATGTCACTG ATGGACAAGT CAGATCTCTT CTTCCATGAC 
TATTCCATAG CACCCCTCTT TGTCCAGGAG AACTACCTGC ATGTAAAGCC TGTGGCTGCA 
GGAGGTGACA TGAAAAAGCA CTTGATGCTT TTAAGCCGGG CAGCAGATAG CATATGTGAC 
GGTGACTTAG TGGACAATCA GATCCGGAGT AAGCAAAACT GGAGTCTTCT GCCCACCCAG 
GCCATCTATG CCAGTGTTCT TCCTGGAGAG TTGATGCGGG GCTACATGAC TCAGTTTCCG 
AGCTTCCCGA GCTGGCTGGG GAAGCACTCG TCTACGGGCA AACATGATCG GATTGTTCAG 
GACCTATCCT TGCACATGAG TCTTAGAACT TACTCCAGCA AAAGGACAGT GAACATGGAT 
TACCTGTCAC ACATCAGAGA CGCACTTGTA CGACCCTTGA CCTCACAAGG GGTAGAAGGG 
GCTCAGCATG TCATCAAACT TATGGACACA TACTATCTGA TGAAAGAAGA TTTTGAGAAC 
ATCATGGAAG TTAGCAGCTG GGGGGGCAAG CCCAGTGCCT TTTCCAAGCT GGATCCCAAG 
GTGAAAGCGG CCTTCACAAG AGCCTACAAT AAGGAGGCCC ACCTGACCCC ATACTCACTT 
CAGGTAGTAA AGACATCGAG GCTCAGCACG GGTCCAGCAC TGGATTCTGA GTACAGTGAG 
GAGTTCCAGG AAGATGACAC TCAGTCTGAG AAAGAGCAGG ATGCAGTGGA GACTGACGCC 
ATGATCAAGA AAAAGACAAG ATCTTCAAAA CCTTCAAAGT CAGAAAGAGA GAAGGAGTCC 
AAAAAAGGAA AAGGAAAGAA TTGGAAGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334287.1
CDS	114..3506
Translation	MDIRKFFGVISSGKKPVNETVKNEKTKASEGTVKGKKGVKEAKVNNSGKEDASKPKQHSKKKRIINDSDSESEETVQVKNAKKKSEKLSLSYKPGKVSQKDPVTYVSETDEDDDFVCKKAASKSKENGVSTNSYLGTSNVKKNEENVKTKNKPLSPIKLTPTSVLDYFGTESVQRSGKKMVTSKRKESSQNTEDSRLNDEAIAKQLQLDEDAELERQLHEDEEFARTLALLDEEPKIKKARKDSEEGEESFSSVQDDLSKAEKQKSPNKAELFSTARKTYSPAKHGKGRASEDAKQPCKSAHRKEACSSPKASAKLALMKAKEESSYNETELLAARRKESATEPKGEKTTPKKTKVSPTKRESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLTFVITGVLESIERDEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKILDEDGLLDLIRTMPGKRSKYEMAAEAEMKKEKSKLERTPQKNDQGKRKISPAKKESESKKCKLTLLKNSPMKAVKKEASTCPRGLDVKETHGNRSSNKEECLLWVDKYKPASLKNIIGQQGDQSCANKLLRWLRNWHKSSPEEKKHAKFGKLASKDDGSSFKAALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKNSLKAVVAESLNNTSIKGFYTSGAAPSVSARHALIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDLRFQRPRVEQIKSAMLSIAFKEGLKIPPPAMNEIILGANQDVRQVLHNLSMWCAQSKALTYDQAKADSQRAKKDIRLGPFDVTRKVFAAGEETAHMSLMDKSDLFFHDYSIAPLFVQENYLHVKPVAAGGDMKKHLMLLSRAADSICDGDLVDNQIRSKQNWSLLPTQAIYASVLPGELMRGYMTQFPSFPSWLGKHSSTGKHDRIVQDLSLHMSLRTYSSKRTVNMDYLSHIRDALVRPLTSQGVEGAQHVIKLMDTYYLMKEDFENIMEVSSWGGKPSAFSKLDPKVKAAFTRAYNKEAHLTPYSLQVVKTSRLSTGPALDSEYSEEFQEDDTQSEKEQDAVETDAMIKKKTRSSKPSKSEREKESKKGKGKNWKK

Target ORF information:

RefSeq Version	NM_001347358.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347358.1

ORF Insert Sequence:

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAA AGTTTGGCAA ACTTGCCAGC 
AAAGATGATG GCTCCAGTTT CAAGGCAGCT CTGCTGTCTG GCCCTCCAGG TGTTGGCAAA 
ACCACTACAG CTTCTCTTGT GTGTCAGGAA TTGGGCTACA GTTATGTGGA ACTAAATGCA 
AGTGATACTC GGAGTAAGAA CAGTTTGAAA GCAGTTGTTG CTGAATCCTT GAACAACACC 
AGCATCAAAG GCTTTTATAC AAGTGGAGCG GCTCCTTCCG TGAGTGCGAG GCACGCCCTC 
ATCATGGATG AGGTCGATGG CATGGCAGGC AATGAAGACA GGGGTGGAAT TCAGGAGCTA 
ATAGGCCTGA TAAAGCATAC TAAAATTCCC ATCATTTGTA TGTGCAATGA TAGAAACCAC 
CCCAAAATTC GCTCATTGGT TCATTATTGT TTTGATCTTC GTTTCCAAAG ACCTCGAGTT 
GAACAGATTA AGAGTGCAAT GCTGTCTATT GCATTTAAAG AGGGTTTAAA AATCCCCCCT 
CCAGCTATGA ATGAAATAAT TTTGGGAGCT AATCAAGATG TCAGACAGGT TTTACACAAT 
CTCAGTATGT GGTGTGCACA AAGTAAGGCC CTGACCTATG ACCAAGCCAA GGCTGATTCT 
CAGAGGGCTA AGAAGGATAT CAGACTGGGC CCATTTGATG TCACTCGGAA AGTGTTTGCA 
GCTGGAGAGG AGACTGCACA CATGTCACTG ATGGACAAGT CAGATCTCTT CTTCCATGAC 
TATTCCATAG CACCCCTCTT TGTCCAGGAG AACTACCTGC ATGTAAAGCC TGTGGCTGCA 
GGAGGTGACA TGAAAAAGCA CTTGATGCTT TTAAGCCGGG CAGCAGATAG CATATGTGAC 
GGTGACTTAG TGGACAATCA GATCCGGAGT AAGCAAAACT GGAGTCTTCT GCCCACCCAG 
GCCATCTATG CCAGTGTTCT TCCTGGAGAG TTGATGCGGG GCTACATGAC TCAGTTTCCG 
AGCTTCCCGA GCTGGCTGGG GAAGCACTCG TCTACGGGCA AACATGATCG GATTGTTCAG 
GACCTATCCT TGCACATGAG TCTTAGAACT TACTCCAGCA AAAGGACAGT GAACATGGAT 
TACCTGTCAC ACATCAGAGA CGCACTTGTA CGACCCTTGA CCTCACAAGG GGTAGAAGGG 
GCTCAGCATG TCATCAAACT TATGGACACA TACTATCTGA TGAAAGAAGA TTTTGAGAAC 
ATCATGGAAG TTAGCAGCTG GGGGGGCAAG CCCAGTGCCT TTTCCAAGCT GGATCCCAAG 
GTGAAAGCGG CCTTCACAAG AGCCTACAAT AAGGAGGCCC ACCTGACCCC ATACTCACTT 
CAGGTAGTAA AGACATCGAG GCTCAGCACG GGTCCAGCAC TGGATTCTGA GTACAGTGAG 
GAGTTCCAGG AAGATGACAC TCAGTCTGAG AAAGAGCAGG ATGCAGTGGA GACTGACGCC 
ATGATCAAGA AAAAGACAAG ATCTTCAAAA CCTTCAAAGT CAGAAAGAGA GAAGGAGTCC 
AAAAAAGGAA AAGGAAAGAA TTGGAAGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rfc1 ( XM_006503801.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503864.1 Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant X2, mRNA.

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CloneID	OMu34983
Clone ID Related Accession (Same CDS sequence)	XM_006503801.3 , XM_006503801.4
Accession Version	XM_006503801.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	replication factor C subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503801.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAA AGTTTGGCAA ACTTGCCAGC 
AAAGATGATG GCTCCAGTTT CAAGGCAGCT CTGCTGTCTG GCCCTCCAGG TGTTGGCAAA 
ACCACTACAG CTTCTCTTGT GTGTCAGGAA TTGGGCTACA GTTATGTGGA ACTAAATGCA 
AGTGATACTC GGAGTAAGAA CAGTTTGAAA GCAGTTGTTG CTGAATCCTT GAACAACACC 
AGCATCAAAG GCTTTTATAC AAGTGGAGCG GCTCCTTCCG TGAGTGCGAG GCACGCCCTC 
ATCATGGATG AGGTCGATGG CATGGCAGGC AATGAAGACA GGGGTGGAAT TCAGGAGCTA 
ATAGGCCTGA TAAAGCATAC TAAAATTCCC ATCATTTGTA TGTGCAATGA TAGAAACCAC 
CCCAAAATTC GCTCATTGGT TCATTATTGT TTTGATCTTC GTTTCCAAAG ACCTCGAGTT 
GAACAGATTA AGAGTGCAAT GCTGTCTATT GCATTTAAAG AGGGTTTAAA AATCCCCCCT 
CCAGCTATGA ATGAAATAAT TTTGGGAGCT AATCAAGATG TCAGACAGGT TTTACACAAT 
CTCAGTATGT GGTGTGCACA AAGTAAGGCC CTGACCTATG ACCAAGCCAA GGCTGATTCT 
CAGAGGGCTA AGAAGGATAT CAGACTGGGC CCATTTGATG TCACTCGGAA AGTGTTTGCA 
GCTGGAGAGG AGACTGCACA CATGTCACTG ATGGACAAGT CAGATCTCTT CTTCCATGAC 
TATTCCATAG CACCCCTCTT TGTCCAGGAG AACTACCTGC ATGTAAAGCC TGTGGCTGCA 
GGAGGTGACA TGAAAAAGCA CTTGATGCTT TTAAGCCGGG CAGCAGATAG CATATGTGAC 
GGTGACTTAG TGGACAATCA GATCCGGAGT AAGCAAAACT GGAGTCTTCT GCCCACCCAG 
GCCATCTATG CCAGTGTTCT TCCTGGAGAG TTGATGCGGG GCTACATGAC TCAGTTTCCG 
AGCTTCCCGA GCTGGCTGGG GAAGCACTCG TCTACGGGCA AACATGATCG GATTGTTCAG 
GACCTATCCT TGCACATGAG TCTTAGAACT TACTCCAGCA AAAGGACAGT GAACATGGAT 
TACCTGTCAC ACATCAGAGA CGCACTTGTA CGACCCTTGA CCTCACAAGG GGTAGAAGGG 
GCTCAGCATG TCATCAAACT TATGGACACA TACTATCTGA TGAAAGAAGA TTTTGAGAAC 
ATCATGGAAG TTAGCAGCTG GGGGGGCAAG CCCAGTGCCT TTTCCAAGCT GGATCCCAAG 
GTGAAAGCGG CCTTCACAAG AGCCTACAAT AAGGAGGCCC ACCTGACCCC ATACTCACTT 
CAGGTAGTAA AGACATCGAG GCTCAGCACG GGTCCAGCAC TGGATTCTGA GTACAGTGAG 
GAGTTCCAGG AAGATGACAC TCAGTCTGAG AAAGAGCAGG ATGCAGTGGA GACTGACGCC 
ATGATCAAGC AGAAAAAGAC AAGATCTTCA AAACCTTCAA AGTCAGAAAG AGAGAAGGAG 
TCCAAAAAAG GAAAAGGAAA GAATTGGAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503864.1
CDS	952..4347
Translation	MDIRKFFGVISSGKKPVNETVKNEKTKASEGTVKGKKGVKEAKVNNSGKEDASKPKQHSKKKRIINDSDSESEETVQVKNAKKKSEKLSLSYKPGKVSQKDPVTYVSETDEDDDFVCKKAASKSKENGVSTNSYLGTSNVKKNEENVKTKNKPLSPIKLTPTSVLDYFGTESVQRSGKKMVTSKRKESSQNTEDSRLNDEAIAKQLQLDEDAELERQLHEDEEFARTLALLDEEPKIKKARKDSEEGEESFSSVQDDLSKAEKQKSPNKAELFSTARKTYSPAKHGKGRASEDAKQPCKSAHRKEACSSPKASAKLALMKAKEESSYNETELLAARRKESATEPKGEKTTPKKTKVSPTKRESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLTFVITGVLESIERDEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKILDEDGLLDLIRTMPGKRSKYEMAAEAEMKKEKSKLERTPQKNDQGKRKISPAKKESESKKCKLTLLKNSPMKAVKKEASTCPRGLDVKETHGNRSSNKEECLLWVDKYKPASLKNIIGQQGDQSCANKLLRWLRNWHKSSPEEKKHAKFGKLASKDDGSSFKAALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKNSLKAVVAESLNNTSIKGFYTSGAAPSVSARHALIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDLRFQRPRVEQIKSAMLSIAFKEGLKIPPPAMNEIILGANQDVRQVLHNLSMWCAQSKALTYDQAKADSQRAKKDIRLGPFDVTRKVFAAGEETAHMSLMDKSDLFFHDYSIAPLFVQENYLHVKPVAAGGDMKKHLMLLSRAADSICDGDLVDNQIRSKQNWSLLPTQAIYASVLPGELMRGYMTQFPSFPSWLGKHSSTGKHDRIVQDLSLHMSLRTYSSKRTVNMDYLSHIRDALVRPLTSQGVEGAQHVIKLMDTYYLMKEDFENIMEVSSWGGKPSAFSKLDPKVKAAFTRAYNKEAHLTPYSLQVVKTSRLSTGPALDSEYSEEFQEDDTQSEKEQDAVETDAMIKQKKTRSSKPSKSEREKESKKGKGKNWKK

Target ORF information:

RefSeq Version	XM_006503801.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus replication factor C (activator 1) 1 (Rfc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503801.3

ORF Insert Sequence:

ATGGACATTC GGAAATTCTT TGGGGTTATA TCAAGTGGAA AAAAGCCTGT AAACGAGACA 
GTAAAGAATG AGAAGACAAA AGCTTCGGAG GGAACTGTCA AAGGAAAAAA GGGAGTCAAG 
GAGGCCAAGG TCAATAACTC CGGTAAAGAG GACGCCTCCA AACCAAAGCA GCACAGCAAG 
AAGAAGAGGA TCATCAATGA CTCAGACTCA GAGTCAGAGG AGACAGTACA GGTAAAAAAT 
GCTAAAAAGA AATCAGAAAA ATTGTCACTG TCTTATAAAC CTGGTAAAGT CTCTCAGAAG 
GATCCTGTCA CCTACGTCTC TGAGACAGAT GAAGATGATG ACTTTGTATG TAAAAAGGCA 
GCCTCCAAAT CAAAAGAGAA TGGAGTATCT ACAAATAGTT ACCTTGGAAC ATCAAACGTG 
AAAAAAAATG AAGAAAACGT TAAGACTAAG AACAAGCCGT TATCTCCAAT AAAACTCACA 
CCAACGTCAG TGCTCGACTA TTTTGGAACT GAAAGTGTCC AGAGATCTGG GAAGAAGATG 
GTGACAAGCA AAAGGAAAGA ATCTTCTCAA AACACAGAGG ATTCCAGATT AAATGATGAG 
GCCATCGCCA AGCAGCTGCA GCTGGATGAA GATGCAGAGC TGGAGAGGCA GTTGCATGAA 
GATGAAGAAT TTGCAAGAAC ACTGGCCTTA TTGGATGAAG AACCCAAGAT CAAAAAGGCC 
CGAAAGGATT CTGAAGAGGG AGAAGAATCA TTTTCATCTG TCCAAGATGA TTTAAGCAAA 
GCAGAAAAGC AGAAAAGCCC TAATAAAGCC GAGCTTTTCT CAACTGCAAG AAAGACCTAC 
AGTCCTGCTA AGCATGGCAA GGGGAGGGCT TCAGAAGATG CTAAGCAGCC TTGCAAATCA 
GCTCACCGGA AGGAAGCCTG TTCCTCACCC AAGGCCAGTG CCAAACTGGC GCTTATGAAA 
GCAAAAGAAG AAAGTTCTTA CAACGAAACA GAGCTGCTGG CTGCAAGAAG AAAAGAAAGC 
GCCACTGAAC CCAAAGGAGA GAAAACAACT CCTAAGAAAA CGAAAGTTTC TCCAACTAAG 
AGAGAGTCTG TAAGCCCAGA AGACTCTGAA AAGAAGCGCA CTAATTATCA AGCTTATCGA 
AGCTACTTGA ACCGAGAAGG TCCCAAAGCT CTGGGCTCCA AAGAAATACC CAAGGGAGCT 
GAAAATTGCT TGGAAGGCCT GACGTTTGTG ATCACAGGAG TGCTGGAGTC CATTGAACGA 
GACGAAGCCA AGTCTCTAAT TGAACGTTAT GGGGGGAAAG TAACAGGAAA CGTGAGCAAG 
AAAACCAACT ACCTCGTCAT GGGCCGCGAC AGTGGGCAGT CCAAGAGTGA CAAGGCAGCA 
GCTCTGGGAA CAAAAATCCT CGATGAAGAC GGCCTGTTGG ATCTGATTCG AACTATGCCA 
GGCAAGAGAT CCAAGTATGA GATGGCTGCT GAGGCTGAGA TGAAGAAAGA AAAGTCCAAA 
TTGGAGCGAA CACCCCAAAA AAATGATCAA GGAAAAAGAA AAATTAGTCC AGCCAAGAAG 
GAGTCAGAGT CTAAAAAGTG CAAGCTGACT CTCCTAAAGA ACAGTCCTAT GAAGGCAGTG 
AAGAAGGAGG CAAGCACGTG CCCAAGGGGC CTGGATGTCA AGGAGACACA TGGCAACCGC 
AGTAGCAACA AAGAGGAATG TCTGCTCTGG GTGGATAAGT ACAAGCCCGC CTCACTCAAG 
AACATAATCG GACAGCAGGG TGACCAGAGC TGTGCCAACA AATTACTACG TTGGCTCAGA 
AACTGGCACA AGAGTTCTCC TGAAGAGAAA AAACATGCAA AGTTTGGCAA ACTTGCCAGC 
AAAGATGATG GCTCCAGTTT CAAGGCAGCT CTGCTGTCTG GCCCTCCAGG TGTTGGCAAA 
ACCACTACAG CTTCTCTTGT GTGTCAGGAA TTGGGCTACA GTTATGTGGA ACTAAATGCA 
AGTGATACTC GGAGTAAGAA CAGTTTGAAA GCAGTTGTTG CTGAATCCTT GAACAACACC 
AGCATCAAAG GCTTTTATAC AAGTGGAGCG GCTCCTTCCG TGAGTGCGAG GCACGCCCTC 
ATCATGGATG AGGTCGATGG CATGGCAGGC AATGAAGACA GGGGTGGAAT TCAGGAGCTA 
ATAGGCCTGA TAAAGCATAC TAAAATTCCC ATCATTTGTA TGTGCAATGA TAGAAACCAC 
CCCAAAATTC GCTCATTGGT TCATTATTGT TTTGATCTTC GTTTCCAAAG ACCTCGAGTT 
GAACAGATTA AGAGTGCAAT GCTGTCTATT GCATTTAAAG AGGGTTTAAA AATCCCCCCT 
CCAGCTATGA ATGAAATAAT TTTGGGAGCT AATCAAGATG TCAGACAGGT TTTACACAAT 
CTCAGTATGT GGTGTGCACA AAGTAAGGCC CTGACCTATG ACCAAGCCAA GGCTGATTCT 
CAGAGGGCTA AGAAGGATAT CAGACTGGGC CCATTTGATG TCACTCGGAA AGTGTTTGCA 
GCTGGAGAGG AGACTGCACA CATGTCACTG ATGGACAAGT CAGATCTCTT CTTCCATGAC 
TATTCCATAG CACCCCTCTT TGTCCAGGAG AACTACCTGC ATGTAAAGCC TGTGGCTGCA 
GGAGGTGACA TGAAAAAGCA CTTGATGCTT TTAAGCCGGG CAGCAGATAG CATATGTGAC 
GGTGACTTAG TGGACAATCA GATCCGGAGT AAGCAAAACT GGAGTCTTCT GCCCACCCAG 
GCCATCTATG CCAGTGTTCT TCCTGGAGAG TTGATGCGGG GCTACATGAC TCAGTTTCCG 
AGCTTCCCGA GCTGGCTGGG GAAGCACTCG TCTACGGGCA AACATGATCG GATTGTTCAG 
GACCTATCCT TGCACATGAG TCTTAGAACT TACTCCAGCA AAAGGACAGT GAACATGGAT 
TACCTGTCAC ACATCAGAGA CGCACTTGTA CGACCCTTGA CCTCACAAGG GGTAGAAGGG 
GCTCAGCATG TCATCAAACT TATGGACACA TACTATCTGA TGAAAGAAGA TTTTGAGAAC 
ATCATGGAAG TTAGCAGCTG GGGGGGCAAG CCCAGTGCCT TTTCCAAGCT GGATCCCAAG 
GTGAAAGCGG CCTTCACAAG AGCCTACAAT AAGGAGGCCC ACCTGACCCC ATACTCACTT 
CAGGTAGTAA AGACATCGAG GCTCAGCACG GGTCCAGCAC TGGATTCTGA GTACAGTGAG 
GAGTTCCAGG AAGATGACAC TCAGTCTGAG AAAGAGCAGG ATGCAGTGGA GACTGACGCC 
ATGATCAAGC AGAAAAAGAC AAGATCTTCA AAACCTTCAA AGTCAGAAAG AGAGAAGGAG 
TCCAAAAAAG GAAAAGGAAA GAATTGGAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Gene expression profiling identifies eleven DNA repair genes down-regulated during mouse neural crest cell migration.
The International journal of developmental biology55(1)65-72(2011)
Albino D,Brizzolara A,Moretti S,Falugi C,Mirisola V,Scaruffi P,Di Candia M,Truini M,Coco S,Bonassi S,Tonini GP

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Genomic analysis of mouse retinal development.
PLoS biology2(9)E247(2004 Sep)
Blackshaw S,Harpavat S,Trimarchi J,Cai L,Huang H,Kuo WP,Weber G,Lee K,Fraioli RE,Cho SH,Yung R,Asch E,Ohno-Machado L,Wong WH,Cepko CL

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Rfc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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