Grhl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grhl1
Entrez Gene ID	195733
Full Name	grainyhead like transcription factor 1
Synonyms	LBP-32,MGR,Tcfcp2l2
General protein information	Preferred Names grainyhead like transcription factor 1 Names grainyhead-like protein 1 homolog grainyhead like 1 grainyhead-like 1 mammalian grainyhead p61 MGR p70 MGR transcription factor CP2-like 2 transcription factor LBP-32
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A1.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030246622.1	XP_030102482.1	grainyhead-like protein 1 homolog isoform X3
XM_030246623.1	XP_030102483.1	grainyhead-like protein 1 homolog isoform X3
NM_145890.2	NP_665897.2	grainyhead-like protein 1 homolog isoform 2
XM_006515016.3	XP_006515079.1	grainyhead-like protein 1 homolog isoform X1
NM_001161406.1	NP_001154878.1	grainyhead-like protein 1 homolog isoform 1
XM_017315004.1	XP_017170493.1	grainyhead-like protein 1 homolog isoform X3
XM_006515016.4	XP_006515079.1	grainyhead-like protein 1 homolog isoform X1
XM_006515017.3	XP_006515080.1	grainyhead-like protein 1 homolog isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Grhl1	NP_001154878.1
Gallus gallus (chicken)	GRHL1	XP_004935809.1
Rattus norvegicus (Norway rat)	Grhl1	XP_234006.5
Homo sapiens (human)	GRHL1	NP_937825.2
Pan troglodytes (chimpanzee)	GRHL1	XP_525685.3
Canis lupus familiaris (dog)	GRHL1	XP_005630168.1
Danio rerio (zebrafish)	grhl1	XP_001923763.1
Xenopus tropicalis (tropical clawed frog)	grhl1	XP_002938935.1
	GRHL1	XP_001090260.2
Bos taurus (cattle)	GRHL1	NP_001069918.1

Gene Ontology
Bibliography

Related articles in PubMed

Grainyhead-like 3 (Grhl3) deficiency in brain leads to altered locomotor activity and decreased anxiety-like behaviors in aged mice.
Dworkin S, Auden A, Partridge DD, Daglas M, Medcalf RL, Mantamadiotis T, Georgy SR, Darido C, Jane SM, Ting SB
Developmental neurobiology77(6)775-788(2017 Jun)

Effects of high and low sodium diet on blood pressure and heart rate in mice lacking the functional grainyhead-like 1 gene.
Walkowska A, Pawlak M, Jane SM, Kompanowska-Jezierska E, Wilanowski T
Physiological research66(1)163-165(2017 Mar)

Two Ancient Gene Families Are Critical for Maintenance of the Mammalian Skin Barrier in Postnatal Life.
Cangkrama M, Darido C, Georgy SR, Partridge D, Auden A, Srivastava S, Wilanowski T, Jane SM
The Journal of investigative dermatology136(7)1438-1448(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Significant changes in blood pressure were observed in mice lacking the GRHl1 gene following high and low sodium diet.
Title: Effects of high and low sodium diet on blood pressure and heart rate in mice lacking the functional grainyhead-like 1 gene.

No involvement of Grhl1 in blood pressure regulation, but Grhl1 expression influences the regulation of heart rate.
Title: Consequences of the loss of the Grainyhead-like 1 gene for renal gene expression, regulation of blood pressure and heart rate in a mouse model.

Loss of Grainy head-like 1 is associated with disruption of the epidermal barrier and squamous cell carcinoma of the skin.
Title: Loss of Grainy head-like 1 is associated with disruption of the epidermal barrier and squamous cell carcinoma of the skin.

Important regulator of the desmoglein 1 genes in the context of hair anchorage and epidermal differentiation.
Title: Perturbed desmosomal cadherin expression in grainy head-like 1-null mice.

Grhl genes are highly expressed in the developing epidermis.
Title: Spatial and temporal expression of the Grainyhead-like transcription factor family during murine development.

The following Grhl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grhl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08139	NM_001161406.1 Latest version!	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$321.30 $459.00
OMu58512	NM_145890.2 Latest version!	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$314.30 $449.00
OMu47264	XM_006515017.3 Latest version!	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu78177	XM_030246623.1 Latest version!	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu78177	XM_017315004.1 Latest version!	Mus musculus grainyhead-like 1 (Drosophila) (Grhl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu78177	XM_030246622.1 Latest version!	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu47263	XM_006515016.4 Latest version!	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu47263	XM_006515016.3	Mus musculus grainyhead-like 1 (Drosophila) (Grhl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grhl1 ( NM_001161406.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001154878.1 Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08139
Clone ID Related Accession (Same CDS sequence)	NM_001161406.1
Accession Version	NM_001161406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	grainyhead-like protein 1 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125067.4, AK161048.1 and DV553123.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF411212.1, AK076307.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACAGG AGTACGACAA CAAAAGGCCC GTGCTGGTAC TTCAGAATGA AGCCCTCTAC 
CCACAGCGGC GCTCCTATAC CAGTGAGGAT GAAGCCTGGA AGTCGTTCCT GGAAAACCCT 
CTCACTGCGG CAACCAAAGC GATGATGAGC ATCAACGGAG ACGAAGACAG CGCGGCTGCG 
CTGGGCCTGC TCTATGACTA CTACAAGGTC CCCAGAGAGC GCCGGTCATC AGCCGTAAAG 
CCCGAGGGAG AGCACCCAGA GCCAGAGCAC AGCAAAAGAA ACAGCATACC AAATGTGACG 
GAGCAGCCCC TCATTTCTGC TGGAGAAAAC AGGGTGCAAG TGCTGAAAAA CGTGCCCTTC 
AACATCGTCC TCCCCCATAG CAACCAGCTG GGCATTGATA AGAGAGGCCA TCTGACAGCT 
CCCGATACAA CAGTCACTGT CTCCATAGCA ACCATGCCTA CCCACTCCAT CAAGACAGAA 
ATCCAGCCGC ACGGCTTTGC TGTGGGAATC CCACCAGCCG TGTACCACTC TGAGCCCACC 
GAACGTGTGG TGGTTTTTGA CCGGAGCCTC AGCACTGATC AGTTCAGCTC TGGCACTCAG 
CCCCCCAATG CTCAGCGGAG GACTCCAGAC TCCACCTTCT CCGAGACCTT CAAGGAGGGC 
GTTCAGGAGG TTTTCTTCCC CTCGGAACTC AGCCTTCGGA TGCCGGGCAT GAATTCAGAG 
GACTATGTCT TTGACAATGT TTCTGGGAAC AACTTTGAGT ATACCCTGGA AGCCTCCAAG 
TCACTGCGGC AGAAGCAAGG GGACAGCACT ATGACATACC TGAATAAAGG CCAGTTCTAT 
CCTGTCACCT TAAAGGAAGG AAGCAGCAAT GAAGGGATTC ACCACCCTAT CAGCAAAGTT 
CGAAGTGTGA TCATGGTGGT TTTTGCTGAA GACAAAAGCA GAGAAGACCA GCTGAGACAC 
TGGAAGTACT GGCACTCCCG TCAGCACACG GCCAAACAGA GGTGCATTGA CATTGCTGAC 
TACAAAGAAA GCTTCAACAC TATCAGCAAC ATTGAGGAGA TAGCTTATAA CGCCATTTCC 
TTCACGTGGG ACATCAATGA TGAGGCAAAG GTCTTCATCT CTGTGAACTG CTTGAGCACA 
GATTTCTCTT CTCAGAAGGG TGTGAAGGGC TTGCCACTCA ACATTCAAAT CGACACATAC 
AGCTATAACA ACCGCAGCAA CAAGCCGGTT CACCGGGCCT ACTGCCAGAT AAAGGTCTTC 
TGCGACAAGG GAGCTGAAAG GAAAATTCGG GATGAAGAAC GAAAACAGAG CAAGAGAAAA 
GTGTCTGACG TTAAAGTGCA GCTGCTTCCC TCGCACAAAC GGACAGACAT CACAGTGTTC 
AAGCCCTTCC TGGACCTCGA CACTCAGCCT GTCCTCTTCA TTCCGGACGT GCATTTTACC 
AACCTGCAGC GGGGCAGTCA TGTTCTTTCC CTCCCCTCTG AAGAACTGGA AGGTGAAGGC 
TCTGTCTTGA AAAGAGGGCC ATTCGGAACC GAAGATGACT TTGGAGTTCC TCCTCCTGCT 
AAGCTGACTC GGACAGAAGA ACCCAAGAGA GTGCTGCTCT ATGTCCGAAA GGAATCAGAA 
GAAGTCTTCG ACGCCCTGAT GCTCAAGACG CCGTCTTTGA AGGGCCTGAT GGAGGCAATT 
TCAGACAAGT ATGATGTCCC CCATGACAAG ATTGGGAAAA TATTTAAGAA GTGCAAAAAA 
GGGATCCTCG TGAACATGGA CGACAACATT GTGAAGCACT ACTCCAATGA GGACACCTTC 
CAGCTGCAGA TAGAGGAAGC CGGCGGCTCG TACAAGCTCA CCCTGACAGA GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154878.1
CDS	153..2009
Translation	MTQEYDNKRPVLVLQNEALYPQRRSYTSEDEAWKSFLENPLTAATKAMMSINGDEDSAAALGLLYDYYKVPRERRSSAVKPEGEHPEPEHSKRNSIPNVTEQPLISAGENRVQVLKNVPFNIVLPHSNQLGIDKRGHLTAPDTTVTVSIATMPTHSIKTEIQPHGFAVGIPPAVYHSEPTERVVVFDRSLSTDQFSSGTQPPNAQRRTPDSTFSETFKEGVQEVFFPSELSLRMPGMNSEDYVFDNVSGNNFEYTLEASKSLRQKQGDSTMTYLNKGQFYPVTLKEGSSNEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDEERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLSLPSEELEGEGSVLKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI

Target ORF information:

RefSeq Version	NM_001161406.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161406.1

ORF Insert Sequence:

ATGACACAGG AGTACGACAA CAAAAGGCCC GTGCTGGTAC TTCAGAATGA AGCCCTCTAC 
CCACAGCGGC GCTCCTATAC CAGTGAGGAT GAAGCCTGGA AGTCGTTCCT GGAAAACCCT 
CTCACTGCGG CAACCAAAGC GATGATGAGC ATCAACGGAG ACGAAGACAG CGCGGCTGCG 
CTGGGCCTGC TCTATGACTA CTACAAGGTC CCCAGAGAGC GCCGGTCATC AGCCGTAAAG 
CCCGAGGGAG AGCACCCAGA GCCAGAGCAC AGCAAAAGAA ACAGCATACC AAATGTGACG 
GAGCAGCCCC TCATTTCTGC TGGAGAAAAC AGGGTGCAAG TGCTGAAAAA CGTGCCCTTC 
AACATCGTCC TCCCCCATAG CAACCAGCTG GGCATTGATA AGAGAGGCCA TCTGACAGCT 
CCCGATACAA CAGTCACTGT CTCCATAGCA ACCATGCCTA CCCACTCCAT CAAGACAGAA 
ATCCAGCCGC ACGGCTTTGC TGTGGGAATC CCACCAGCCG TGTACCACTC TGAGCCCACC 
GAACGTGTGG TGGTTTTTGA CCGGAGCCTC AGCACTGATC AGTTCAGCTC TGGCACTCAG 
CCCCCCAATG CTCAGCGGAG GACTCCAGAC TCCACCTTCT CCGAGACCTT CAAGGAGGGC 
GTTCAGGAGG TTTTCTTCCC CTCGGAACTC AGCCTTCGGA TGCCGGGCAT GAATTCAGAG 
GACTATGTCT TTGACAATGT TTCTGGGAAC AACTTTGAGT ATACCCTGGA AGCCTCCAAG 
TCACTGCGGC AGAAGCAAGG GGACAGCACT ATGACATACC TGAATAAAGG CCAGTTCTAT 
CCTGTCACCT TAAAGGAAGG AAGCAGCAAT GAAGGGATTC ACCACCCTAT CAGCAAAGTT 
CGAAGTGTGA TCATGGTGGT TTTTGCTGAA GACAAAAGCA GAGAAGACCA GCTGAGACAC 
TGGAAGTACT GGCACTCCCG TCAGCACACG GCCAAACAGA GGTGCATTGA CATTGCTGAC 
TACAAAGAAA GCTTCAACAC TATCAGCAAC ATTGAGGAGA TAGCTTATAA CGCCATTTCC 
TTCACGTGGG ACATCAATGA TGAGGCAAAG GTCTTCATCT CTGTGAACTG CTTGAGCACA 
GATTTCTCTT CTCAGAAGGG TGTGAAGGGC TTGCCACTCA ACATTCAAAT CGACACATAC 
AGCTATAACA ACCGCAGCAA CAAGCCGGTT CACCGGGCCT ACTGCCAGAT AAAGGTCTTC 
TGCGACAAGG GAGCTGAAAG GAAAATTCGG GATGAAGAAC GAAAACAGAG CAAGAGAAAA 
GTGTCTGACG TTAAAGTGCA GCTGCTTCCC TCGCACAAAC GGACAGACAT CACAGTGTTC 
AAGCCCTTCC TGGACCTCGA CACTCAGCCT GTCCTCTTCA TTCCGGACGT GCATTTTACC 
AACCTGCAGC GGGGCAGTCA TGTTCTTTCC CTCCCCTCTG AAGAACTGGA AGGTGAAGGC 
TCTGTCTTGA AAAGAGGGCC ATTCGGAACC GAAGATGACT TTGGAGTTCC TCCTCCTGCT 
AAGCTGACTC GGACAGAAGA ACCCAAGAGA GTGCTGCTCT ATGTCCGAAA GGAATCAGAA 
GAAGTCTTCG ACGCCCTGAT GCTCAAGACG CCGTCTTTGA AGGGCCTGAT GGAGGCAATT 
TCAGACAAGT ATGATGTCCC CCATGACAAG ATTGGGAAAA TATTTAAGAA GTGCAAAAAA 
GGGATCCTCG TGAACATGGA CGACAACATT GTGAAGCACT ACTCCAATGA GGACACCTTC 
CAGCTGCAGA TAGAGGAAGC CGGCGGCTCG TACAAGCTCA CCCTGACAGA GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grhl1 ( NM_145890.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_665897.2 Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu58512
Clone ID Related Accession (Same CDS sequence)	NM_145890.2
Accession Version	NM_145890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	grainyhead-like protein 1 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125067.4. On May 30, 2009 this sequence version replaced NM_145890.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform 92) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF411211.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTCTCC TTGATGATGA ACTGTGTGAC CTAAACAGCA TACCAAATGT GACGGAGCAG 
CCCCTCATTT CTGCTGGAGA AAACAGGGTG CAAGTGCTGA AAAACGTGCC CTTCAACATC 
GTCCTCCCCC ATAGCAACCA GCTGGGCATT GATAAGAGAG GCCATCTGAC AGCTCCCGAT 
ACAACAGTCA CTGTCTCCAT AGCAACCATG CCTACCCACT CCATCAAGAC AGAAATCCAG 
CCGCACGGCT TTGCTGTGGG AATCCCACCA GCCGTGTACC ACTCTGAGCC CACCGAACGT 
GTGGTGGTTT TTGACCGGAG CCTCAGCACT GATCAGTTCA GCTCTGGCAC TCAGCCCCCC 
AATGCTCAGC GGAGGACTCC AGACTCCACC TTCTCCGAGA CCTTCAAGGA GGGCGTTCAG 
GAGGTTTTCT TCCCCTCGGA ACTCAGCCTT CGGATGCCGG GCATGAATTC AGAGGACTAT 
GTCTTTGACA ATGTTTCTGG GAACAACTTT GAGTATACCC TGGAAGCCTC CAAGTCACTG 
CGGCAGAAGC AAGGGGACAG CACTATGACA TACCTGAATA AAGGCCAGTT CTATCCTGTC 
ACCTTAAAGG AAGGAAGCAG CAATGAAGGG ATTCACCACC CTATCAGCAA AGTTCGAAGT 
GTGATCATGG TGGTTTTTGC TGAAGACAAA AGCAGAGAAG ACCAGCTGAG ACACTGGAAG 
TACTGGCACT CCCGTCAGCA CACGGCCAAA CAGAGGTGCA TTGACATTGC TGACTACAAA 
GAAAGCTTCA ACACTATCAG CAACATTGAG GAGATAGCTT ATAACGCCAT TTCCTTCACG 
TGGGACATCA ATGATGAGGC AAAGGTCTTC ATCTCTGTGA ACTGCTTGAG CACAGATTTC 
TCTTCTCAGA AGGGTGTGAA GGGCTTGCCA CTCAACATTC AAATCGACAC ATACAGCTAT 
AACAACCGCA GCAACAAGCC GGTTCACCGG GCCTACTGCC AGATAAAGGT CTTCTGCGAC 
AAGGGAGCTG AAAGGAAAAT TCGGGATGAA GAACGAAAAC AGAGCAAGAG AAAAGTGTCT 
GACGTTAAAG TGCAGCTGCT TCCCTCGCAC AAACGGACAG ACATCACAGT GTTCAAGCCC 
TTCCTGGACC TCGACACTCA GCCTGTCCTC TTCATTCCGG ACGTGCATTT TACCAACCTG 
CAGCGGGGCA GTCATGTTCT TTCCCTCCCC TCTGAAGAAC TGGAAGGTGA AGGCTCTGTC 
TTGAAAAGAG GGCCATTCGG AACCGAAGAT GACTTTGGAG TTCCTCCTCC TGCTAAGCTG 
ACTCGGACAG AAGAACCCAA GAGAGTGCTG CTCTATGTCC GAAAGGAATC AGAAGAAGTC 
TTCGACGCCC TGATGCTCAA GACGCCGTCT TTGAAGGGCC TGATGGAGGC AATTTCAGAC 
AAGTATGATG TCCCCCATGA CAAGATTGGG AAAATATTTA AGAAGTGCAA AAAAGGGATC 
CTCGTGAACA TGGACGACAA CATTGTGAAG CACTACTCCA ATGAGGACAC CTTCCAGCTG 
CAGATAGAGG AAGCCGGCGG CTCGTACAAG CTCACCCTGA CAGAGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665897.2
CDS	31..1641
Translation	MVLLDDELCDLNSIPNVTEQPLISAGENRVQVLKNVPFNIVLPHSNQLGIDKRGHLTAPDTTVTVSIATMPTHSIKTEIQPHGFAVGIPPAVYHSEPTERVVVFDRSLSTDQFSSGTQPPNAQRRTPDSTFSETFKEGVQEVFFPSELSLRMPGMNSEDYVFDNVSGNNFEYTLEASKSLRQKQGDSTMTYLNKGQFYPVTLKEGSSNEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDEERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLSLPSEELEGEGSVLKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI

Target ORF information:

RefSeq Version	NM_145890.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145890.2

ORF Insert Sequence:

ATGGTTCTCC TTGATGATGA ACTGTGTGAC CTAAACAGCA TACCAAATGT GACGGAGCAG 
CCCCTCATTT CTGCTGGAGA AAACAGGGTG CAAGTGCTGA AAAACGTGCC CTTCAACATC 
GTCCTCCCCC ATAGCAACCA GCTGGGCATT GATAAGAGAG GCCATCTGAC AGCTCCCGAT 
ACAACAGTCA CTGTCTCCAT AGCAACCATG CCTACCCACT CCATCAAGAC AGAAATCCAG 
CCGCACGGCT TTGCTGTGGG AATCCCACCA GCCGTGTACC ACTCTGAGCC CACCGAACGT 
GTGGTGGTTT TTGACCGGAG CCTCAGCACT GATCAGTTCA GCTCTGGCAC TCAGCCCCCC 
AATGCTCAGC GGAGGACTCC AGACTCCACC TTCTCCGAGA CCTTCAAGGA GGGCGTTCAG 
GAGGTTTTCT TCCCCTCGGA ACTCAGCCTT CGGATGCCGG GCATGAATTC AGAGGACTAT 
GTCTTTGACA ATGTTTCTGG GAACAACTTT GAGTATACCC TGGAAGCCTC CAAGTCACTG 
CGGCAGAAGC AAGGGGACAG CACTATGACA TACCTGAATA AAGGCCAGTT CTATCCTGTC 
ACCTTAAAGG AAGGAAGCAG CAATGAAGGG ATTCACCACC CTATCAGCAA AGTTCGAAGT 
GTGATCATGG TGGTTTTTGC TGAAGACAAA AGCAGAGAAG ACCAGCTGAG ACACTGGAAG 
TACTGGCACT CCCGTCAGCA CACGGCCAAA CAGAGGTGCA TTGACATTGC TGACTACAAA 
GAAAGCTTCA ACACTATCAG CAACATTGAG GAGATAGCTT ATAACGCCAT TTCCTTCACG 
TGGGACATCA ATGATGAGGC AAAGGTCTTC ATCTCTGTGA ACTGCTTGAG CACAGATTTC 
TCTTCTCAGA AGGGTGTGAA GGGCTTGCCA CTCAACATTC AAATCGACAC ATACAGCTAT 
AACAACCGCA GCAACAAGCC GGTTCACCGG GCCTACTGCC AGATAAAGGT CTTCTGCGAC 
AAGGGAGCTG AAAGGAAAAT TCGGGATGAA GAACGAAAAC AGAGCAAGAG AAAAGTGTCT 
GACGTTAAAG TGCAGCTGCT TCCCTCGCAC AAACGGACAG ACATCACAGT GTTCAAGCCC 
TTCCTGGACC TCGACACTCA GCCTGTCCTC TTCATTCCGG ACGTGCATTT TACCAACCTG 
CAGCGGGGCA GTCATGTTCT TTCCCTCCCC TCTGAAGAAC TGGAAGGTGA AGGCTCTGTC 
TTGAAAAGAG GGCCATTCGG AACCGAAGAT GACTTTGGAG TTCCTCCTCC TGCTAAGCTG 
ACTCGGACAG AAGAACCCAA GAGAGTGCTG CTCTATGTCC GAAAGGAATC AGAAGAAGTC 
TTCGACGCCC TGATGCTCAA GACGCCGTCT TTGAAGGGCC TGATGGAGGC AATTTCAGAC 
AAGTATGATG TCCCCCATGA CAAGATTGGG AAAATATTTA AGAAGTGCAA AAAAGGGATC 
CTCGTGAACA TGGACGACAA CATTGTGAAG CACTACTCCA ATGAGGACAC CTTCCAGCTG 
CAGATAGAGG AAGCCGGCGG CTCGTACAAG CTCACCCTGA CAGAGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grhl1 ( XM_006515016.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515079.1 Mus musculus grainyhead-like 1 (Drosophila) (Grhl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47263
Clone ID Related Accession (Same CDS sequence)	XM_006515016.3 , XM_006515016.4
Accession Version	XM_006515016.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	grainyhead-like protein 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515016.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACAGG AGTACGACAA CAAAAGGCCC GTGCTGGTAC TTCAGAATGA AGCCCTCTAC 
CCACAGCGGC GCTCCTATAC CAGTGAGGAT GAAGCCTGGA AGTCGTTCCT GGAAAACCCT 
CTCACTGCGG CAACCAAAGC GATGATGAGC ATCAACGGAG ACGAAGACAG CGCGGCTGCG 
CTGGGCCTGC TCTATGACTA CTACAAGGTC CCCAGAGAGC GCCGGTCATC AGCCGTAAAG 
CCCGAGGGAG AGCACCCAGA GCCAGAGCAC AGCAAAAGCA TACCAAATGT GACGGAGCAG 
CCCCTCATTT CTGCTGGAGA AAACAGGGTG CAAGTGCTGA AAAACGTGCC CTTCAACATC 
GTCCTCCCCC ATAGCAACCA GCTGGGCATT GATAAGAGAG GCCATCTGAC AGCTCCCGAT 
ACAACAGTCA CTGTCTCCAT AGCAACCATG CCTACCCACT CCATCAAGAC AGAAATCCAG 
CCGCACGGCT TTGCTGTGGG AATCCCACCA GCCGTGTACC ACTCTGAGCC CACCGAACGT 
GTGGTGGTTT TTGACCGGAG CCTCAGCACT GATCAGTTCA GCTCTGGCAC TCAGCCCCCC 
AATGCTCAGC GGAGGACTCC AGACTCCACC TTCTCCGAGA CCTTCAAGGA GGGCGTTCAG 
GAGGTTTTCT TCCCCTCGGA ACTCAGCCTT CGGATGCCGG GCATGAATTC AGAGGACTAT 
GTCTTTGACA ATGTTTCTGG GAACAACTTT GAGTATACCC TGGAAGCCTC CAAGTCACTG 
CGGCAGAAGC AAGGGGACAG CACTATGACA TACCTGAATA AAGGCCAGTT CTATCCTGTC 
ACCTTAAAGG AAGGAAGCAG CAATGAAGGG ATTCACCACC CTATCAGCAA AGTTCGAAGT 
GTGATCATGG TGGTTTTTGC TGAAGACAAA AGCAGAGAAG ACCAGCTGAG ACACTGGAAG 
TACTGGCACT CCCGTCAGCA CACGGCCAAA CAGAGGTGCA TTGACATTGC TGACTACAAA 
GAAAGCTTCA ACACTATCAG CAACATTGAG GAGATAGCTT ATAACGCCAT TTCCTTCACG 
TGGGACATCA ATGATGAGGC AAAGGTCTTC ATCTCTGTGA ACTGCTTGAG CACAGATTTC 
TCTTCTCAGA AGGGTGTGAA GGGCTTGCCA CTCAACATTC AAATCGACAC ATACAGCTAT 
AACAACCGCA GCAACAAGCC GGTTCACCGG GCCTACTGCC AGATAAAGGT CTTCTGCGAC 
AAGGGAGCTG AAAGGAAAAT TCGGGATGAA GAACGAAAAC AGAGCAAGAG AAAAGTGTCT 
GACGTTAAAG TGCAGCTGCT TCCCTCGCAC AAACGGACAG ACATCACAGT GTTCAAGCCC 
TTCCTGGACC TCGACACTCA GCCTGTCCTC TTCATTCCGG ACGTGCATTT TACCAACCTG 
CAGCGGGGCA GTCATGTTCT TTCCCTCCCC TCTGAAGAAC TGGAAGGTGA AGGCTCTGTC 
TTGAAAAGAG GGCCATTCGG AACCGAAGAT GACTTTGGAG TTCCTCCTCC TGCTAAGCTG 
ACTCGGACAG AAGAACCCAA GAGAGTGCTG CTCTATGTCC GAAAGGAATC AGAAGAAGTC 
TTCGACGCCC TGATGCTCAA GACGCCGTCT TTGAAGGGCC TGATGGAGGC AATTTCAGAC 
AAGTATGATG TCCCCCATGA CAAGATTGGG AAAATATTTA AGAAGTGCAA AAAAGGGATC 
CTCGTGAACA TGGACGACAA CATTGTGAAG CACTACTCCA ATGAGGACAC CTTCCAGCTG 
CAGATAGAGG AAGCCGGCGG CTCGTACAAG CTCACCCTGA CAGAGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515079.1
CDS	211..2061
Translation	MTQEYDNKRPVLVLQNEALYPQRRSYTSEDEAWKSFLENPLTAATKAMMSINGDEDSAAALGLLYDYYKVPRERRSSAVKPEGEHPEPEHSKSIPNVTEQPLISAGENRVQVLKNVPFNIVLPHSNQLGIDKRGHLTAPDTTVTVSIATMPTHSIKTEIQPHGFAVGIPPAVYHSEPTERVVVFDRSLSTDQFSSGTQPPNAQRRTPDSTFSETFKEGVQEVFFPSELSLRMPGMNSEDYVFDNVSGNNFEYTLEASKSLRQKQGDSTMTYLNKGQFYPVTLKEGSSNEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDEERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLSLPSEELEGEGSVLKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI

Target ORF information:

RefSeq Version	XM_006515016.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus grainyhead-like 1 (Drosophila) (Grhl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515016.3

ORF Insert Sequence:

ATGACACAGG AGTACGACAA CAAAAGGCCC GTGCTGGTAC TTCAGAATGA AGCCCTCTAC 
CCACAGCGGC GCTCCTATAC CAGTGAGGAT GAAGCCTGGA AGTCGTTCCT GGAAAACCCT 
CTCACTGCGG CAACCAAAGC GATGATGAGC ATCAACGGAG ACGAAGACAG CGCGGCTGCG 
CTGGGCCTGC TCTATGACTA CTACAAGGTC CCCAGAGAGC GCCGGTCATC AGCCGTAAAG 
CCCGAGGGAG AGCACCCAGA GCCAGAGCAC AGCAAAAGCA TACCAAATGT GACGGAGCAG 
CCCCTCATTT CTGCTGGAGA AAACAGGGTG CAAGTGCTGA AAAACGTGCC CTTCAACATC 
GTCCTCCCCC ATAGCAACCA GCTGGGCATT GATAAGAGAG GCCATCTGAC AGCTCCCGAT 
ACAACAGTCA CTGTCTCCAT AGCAACCATG CCTACCCACT CCATCAAGAC AGAAATCCAG 
CCGCACGGCT TTGCTGTGGG AATCCCACCA GCCGTGTACC ACTCTGAGCC CACCGAACGT 
GTGGTGGTTT TTGACCGGAG CCTCAGCACT GATCAGTTCA GCTCTGGCAC TCAGCCCCCC 
AATGCTCAGC GGAGGACTCC AGACTCCACC TTCTCCGAGA CCTTCAAGGA GGGCGTTCAG 
GAGGTTTTCT TCCCCTCGGA ACTCAGCCTT CGGATGCCGG GCATGAATTC AGAGGACTAT 
GTCTTTGACA ATGTTTCTGG GAACAACTTT GAGTATACCC TGGAAGCCTC CAAGTCACTG 
CGGCAGAAGC AAGGGGACAG CACTATGACA TACCTGAATA AAGGCCAGTT CTATCCTGTC 
ACCTTAAAGG AAGGAAGCAG CAATGAAGGG ATTCACCACC CTATCAGCAA AGTTCGAAGT 
GTGATCATGG TGGTTTTTGC TGAAGACAAA AGCAGAGAAG ACCAGCTGAG ACACTGGAAG 
TACTGGCACT CCCGTCAGCA CACGGCCAAA CAGAGGTGCA TTGACATTGC TGACTACAAA 
GAAAGCTTCA ACACTATCAG CAACATTGAG GAGATAGCTT ATAACGCCAT TTCCTTCACG 
TGGGACATCA ATGATGAGGC AAAGGTCTTC ATCTCTGTGA ACTGCTTGAG CACAGATTTC 
TCTTCTCAGA AGGGTGTGAA GGGCTTGCCA CTCAACATTC AAATCGACAC ATACAGCTAT 
AACAACCGCA GCAACAAGCC GGTTCACCGG GCCTACTGCC AGATAAAGGT CTTCTGCGAC 
AAGGGAGCTG AAAGGAAAAT TCGGGATGAA GAACGAAAAC AGAGCAAGAG AAAAGTGTCT 
GACGTTAAAG TGCAGCTGCT TCCCTCGCAC AAACGGACAG ACATCACAGT GTTCAAGCCC 
TTCCTGGACC TCGACACTCA GCCTGTCCTC TTCATTCCGG ACGTGCATTT TACCAACCTG 
CAGCGGGGCA GTCATGTTCT TTCCCTCCCC TCTGAAGAAC TGGAAGGTGA AGGCTCTGTC 
TTGAAAAGAG GGCCATTCGG AACCGAAGAT GACTTTGGAG TTCCTCCTCC TGCTAAGCTG 
ACTCGGACAG AAGAACCCAA GAGAGTGCTG CTCTATGTCC GAAAGGAATC AGAAGAAGTC 
TTCGACGCCC TGATGCTCAA GACGCCGTCT TTGAAGGGCC TGATGGAGGC AATTTCAGAC 
AAGTATGATG TCCCCCATGA CAAGATTGGG AAAATATTTA AGAAGTGCAA AAAAGGGATC 
CTCGTGAACA TGGACGACAA CATTGTGAAG CACTACTCCA ATGAGGACAC CTTCCAGCTG 
CAGATAGAGG AAGCCGGCGG CTCGTACAAG CTCACCCTGA CAGAGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grhl1 ( XM_006515017.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515080.1 Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X2, mRNA.

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CloneID	OMu47264
Clone ID Related Accession (Same CDS sequence)	XM_006515017.3
Accession Version	XM_006515017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	grainyhead-like protein 1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGCA TCAACGGAGA CGAAGACAGC GCGGCTGCGC TGGGCCTGCT CTATGACTAC 
TACAAGGTCC CCAGAGAGCG CCGGTCATCA GCCGTAAAGC CCGAGGGAGA GCACCCAGAG 
CCAGAGCACA GCAAAAGAAA CAGCATACCA AATGTGACGG AGCAGCCCCT CATTTCTGCT 
GGAGAAAACA GGGTGCAAGT GCTGAAAAAC GTGCCCTTCA ACATCGTCCT CCCCCATAGC 
AACCAGCTGG GCATTGATAA GAGAGGCCAT CTGACAGCTC CCGATACAAC AGTCACTGTC 
TCCATAGCAA CCATGCCTAC CCACTCCATC AAGACAGAAA TCCAGCCGCA CGGCTTTGCT 
GTGGGAATCC CACCAGCCGT GTACCACTCT GAGCCCACCG AACGTGTGGT GGTTTTTGAC 
CGGAGCCTCA GCACTGATCA GTTCAGCTCT GGCACTCAGC CCCCCAATGC TCAGCGGAGG 
ACTCCAGACT CCACCTTCTC CGAGACCTTC AAGGAGGGCG TTCAGGAGGT TTTCTTCCCC 
TCGGAACTCA GCCTTCGGAT GCCGGGCATG AATTCAGAGG ACTATGTCTT TGACAATGTT 
TCTGGGAACA ACTTTGAGTA TACCCTGGAA GCCTCCAAGT CACTGCGGCA GAAGCAAGGG 
GACAGCACTA TGACATACCT GAATAAAGGC CAGTTCTATC CTGTCACCTT AAAGGAAGGA 
AGCAGCAATG AAGGGATTCA CCACCCTATC AGCAAAGTTC GAAGTGTGAT CATGGTGGTT 
TTTGCTGAAG ACAAAAGCAG AGAAGACCAG CTGAGACACT GGAAGTACTG GCACTCCCGT 
CAGCACACGG CCAAACAGAG GTGCATTGAC ATTGCTGACT ACAAAGAAAG CTTCAACACT 
ATCAGCAACA TTGAGGAGAT AGCTTATAAC GCCATTTCCT TCACGTGGGA CATCAATGAT 
GAGGCAAAGG TCTTCATCTC TGTGAACTGC TTGAGCACAG ATTTCTCTTC TCAGAAGGGT 
GTGAAGGGCT TGCCACTCAA CATTCAAATC GACACATACA GCTATAACAA CCGCAGCAAC 
AAGCCGGTTC ACCGGGCCTA CTGCCAGATA AAGGTCTTCT GCGACAAGGG AGCTGAAAGG 
AAAATTCGGG ATGAAGAACG AAAACAGAGC AAGAGAAAAG TGTCTGACGT TAAAGTGCAG 
CTGCTTCCCT CGCACAAACG GACAGACATC ACAGTGTTCA AGCCCTTCCT GGACCTCGAC 
ACTCAGCCTG TCCTCTTCAT TCCGGACGTG CATTTTACCA ACCTGCAGCG GGGCAGTCAT 
GTTCTTTCCC TCCCCTCTGA AGAACTGGAA GGTGAAGGCT CTGTCTTGAA AAGAGGGCCA 
TTCGGAACCG AAGATGACTT TGGAGTTCCT CCTCCTGCTA AGCTGACTCG GACAGAAGAA 
CCCAAGAGAG TGCTGCTCTA TGTCCGAAAG GAATCAGAAG AAGTCTTCGA CGCCCTGATG 
CTCAAGACGC CGTCTTTGAA GGGCCTGATG GAGGCAATTT CAGACAAGTA TGATGTCCCC 
CATGACAAGA TTGGGAAAAT ATTTAAGAAG TGCAAAAAAG GGATCCTCGT GAACATGGAC 
GACAACATTG TGAAGCACTA CTCCAATGAG GACACCTTCC AGCTGCAGAT AGAGGAAGCC 
GGCGGCTCGT ACAAGCTCAC CCTGACAGAG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515080.1
CDS	344..2059
Translation	MMSINGDEDSAAALGLLYDYYKVPRERRSSAVKPEGEHPEPEHSKRNSIPNVTEQPLISAGENRVQVLKNVPFNIVLPHSNQLGIDKRGHLTAPDTTVTVSIATMPTHSIKTEIQPHGFAVGIPPAVYHSEPTERVVVFDRSLSTDQFSSGTQPPNAQRRTPDSTFSETFKEGVQEVFFPSELSLRMPGMNSEDYVFDNVSGNNFEYTLEASKSLRQKQGDSTMTYLNKGQFYPVTLKEGSSNEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDEERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLSLPSEELEGEGSVLKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI

Target ORF information:

RefSeq Version	XM_006515017.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515017.3

ORF Insert Sequence:

ATGATGAGCA TCAACGGAGA CGAAGACAGC GCGGCTGCGC TGGGCCTGCT CTATGACTAC 
TACAAGGTCC CCAGAGAGCG CCGGTCATCA GCCGTAAAGC CCGAGGGAGA GCACCCAGAG 
CCAGAGCACA GCAAAAGAAA CAGCATACCA AATGTGACGG AGCAGCCCCT CATTTCTGCT 
GGAGAAAACA GGGTGCAAGT GCTGAAAAAC GTGCCCTTCA ACATCGTCCT CCCCCATAGC 
AACCAGCTGG GCATTGATAA GAGAGGCCAT CTGACAGCTC CCGATACAAC AGTCACTGTC 
TCCATAGCAA CCATGCCTAC CCACTCCATC AAGACAGAAA TCCAGCCGCA CGGCTTTGCT 
GTGGGAATCC CACCAGCCGT GTACCACTCT GAGCCCACCG AACGTGTGGT GGTTTTTGAC 
CGGAGCCTCA GCACTGATCA GTTCAGCTCT GGCACTCAGC CCCCCAATGC TCAGCGGAGG 
ACTCCAGACT CCACCTTCTC CGAGACCTTC AAGGAGGGCG TTCAGGAGGT TTTCTTCCCC 
TCGGAACTCA GCCTTCGGAT GCCGGGCATG AATTCAGAGG ACTATGTCTT TGACAATGTT 
TCTGGGAACA ACTTTGAGTA TACCCTGGAA GCCTCCAAGT CACTGCGGCA GAAGCAAGGG 
GACAGCACTA TGACATACCT GAATAAAGGC CAGTTCTATC CTGTCACCTT AAAGGAAGGA 
AGCAGCAATG AAGGGATTCA CCACCCTATC AGCAAAGTTC GAAGTGTGAT CATGGTGGTT 
TTTGCTGAAG ACAAAAGCAG AGAAGACCAG CTGAGACACT GGAAGTACTG GCACTCCCGT 
CAGCACACGG CCAAACAGAG GTGCATTGAC ATTGCTGACT ACAAAGAAAG CTTCAACACT 
ATCAGCAACA TTGAGGAGAT AGCTTATAAC GCCATTTCCT TCACGTGGGA CATCAATGAT 
GAGGCAAAGG TCTTCATCTC TGTGAACTGC TTGAGCACAG ATTTCTCTTC TCAGAAGGGT 
GTGAAGGGCT TGCCACTCAA CATTCAAATC GACACATACA GCTATAACAA CCGCAGCAAC 
AAGCCGGTTC ACCGGGCCTA CTGCCAGATA AAGGTCTTCT GCGACAAGGG AGCTGAAAGG 
AAAATTCGGG ATGAAGAACG AAAACAGAGC AAGAGAAAAG TGTCTGACGT TAAAGTGCAG 
CTGCTTCCCT CGCACAAACG GACAGACATC ACAGTGTTCA AGCCCTTCCT GGACCTCGAC 
ACTCAGCCTG TCCTCTTCAT TCCGGACGTG CATTTTACCA ACCTGCAGCG GGGCAGTCAT 
GTTCTTTCCC TCCCCTCTGA AGAACTGGAA GGTGAAGGCT CTGTCTTGAA AAGAGGGCCA 
TTCGGAACCG AAGATGACTT TGGAGTTCCT CCTCCTGCTA AGCTGACTCG GACAGAAGAA 
CCCAAGAGAG TGCTGCTCTA TGTCCGAAAG GAATCAGAAG AAGTCTTCGA CGCCCTGATG 
CTCAAGACGC CGTCTTTGAA GGGCCTGATG GAGGCAATTT CAGACAAGTA TGATGTCCCC 
CATGACAAGA TTGGGAAAAT ATTTAAGAAG TGCAAAAAAG GGATCCTCGT GAACATGGAC 
GACAACATTG TGAAGCACTA CTCCAATGAG GACACCTTCC AGCTGCAGAT AGAGGAAGCC 
GGCGGCTCGT ACAAGCTCAC CCTGACAGAG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grhl1 ( XM_030246622.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102482.1 Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu78177
Clone ID Related Accession (Same CDS sequence)	XM_030246622.1 , XM_030246623.1 , XM_017315004.1
Accession Version	XM_030246622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	grainyhead-like protein 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCG GAGGACTCCA GACTCCACCT TCTCCGAGAC CTTCAAGGAG GGCGTTCAGG 
AGGAACAACT TTGAGTATAC CCTGGAAGCC TCCAAGTCAC TGCGGCAGAA GCAAGGGGAC 
AGCACTATGA CATACCTGAA TAAAGGCCAG TTCTATCCTG TCACCTTAAA GGAAGGAAGC 
AGCAATGAAG GGATTCACCA CCCTATCAGC AAAGTTCGAA GTGTGATCAT GGTGGTTTTT 
GCTGAAGACA AAAGCAGAGA AGACCAGCTG AGACACTGGA AGTACTGGCA CTCCCGTCAG 
CACACGGCCA AACAGAGGTG CATTGACATT GCTGACTACA AAGAAAGCTT CAACACTATC 
AGCAACATTG AGGAGATAGC TTATAACGCC ATTTCCTTCA CGTGGGACAT CAATGATGAG 
GCAAAGGTCT TCATCTCTGT GAACTGCTTG AGCACAGATT TCTCTTCTCA GAAGGGTGTG 
AAGGGCTTGC CACTCAACAT TCAAATCGAC ACATACAGCT ATAACAACCG CAGCAACAAG 
CCGGTTCACC GGGCCTACTG CCAGATAAAG GTCTTCTGCG ACAAGGGAGC TGAAAGGAAA 
ATTCGGGATG AAGAACGAAA ACAGAGCAAG AGAAAAGTGT CTGACGTTAA AGTGCAGCTG 
CTTCCCTCGC ACAAACGGAC AGACATCACA GTGTTCAAGC CCTTCCTGGA CCTCGACACT 
CAGCCTGTCC TCTTCATTCC GGACGTGCAT TTTACCAACC TGCAGCGGGG CAGTCATGTT 
CTTTCCCTCC CCTCTGAAGA ACTGGAAGGT GAAGGCTCTG TCTTGAAAAG AGGGCCATTC 
GGAACCGAAG ATGACTTTGG AGTTCCTCCT CCTGCTAAGC TGACTCGGAC AGAAGAACCC 
AAGAGAGTGC TGCTCTATGT CCGAAAGGAA TCAGAAGAAG TCTTCGACGC CCTGATGCTC 
AAGACGCCGT CTTTGAAGGG CCTGATGGAG GCAATTTCAG ACAAGTATGA TGTCCCCCAT 
GACAAGATTG GGAAAATATT TAAGAAGTGC AAAAAAGGGA TCCTCGTGAA CATGGACGAC 
AACATTGTGA AGCACTACTC CAATGAGGAC ACCTTCCAGC TGCAGATAGA GGAAGCCGGC 
GGCTCGTACA AGCTCACCCT GACAGAGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102482.1
CDS	815..1987
Translation	MLSGGLQTPPSPRPSRRAFRRNNFEYTLEASKSLRQKQGDSTMTYLNKGQFYPVTLKEGSSNEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDEERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLSLPSEELEGEGSVLKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI

Target ORF information:

RefSeq Version	XM_030246622.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246622.1

ORF Insert Sequence:

ATGCTCAGCG GAGGACTCCA GACTCCACCT TCTCCGAGAC CTTCAAGGAG GGCGTTCAGG 
AGGAACAACT TTGAGTATAC CCTGGAAGCC TCCAAGTCAC TGCGGCAGAA GCAAGGGGAC 
AGCACTATGA CATACCTGAA TAAAGGCCAG TTCTATCCTG TCACCTTAAA GGAAGGAAGC 
AGCAATGAAG GGATTCACCA CCCTATCAGC AAAGTTCGAA GTGTGATCAT GGTGGTTTTT 
GCTGAAGACA AAAGCAGAGA AGACCAGCTG AGACACTGGA AGTACTGGCA CTCCCGTCAG 
CACACGGCCA AACAGAGGTG CATTGACATT GCTGACTACA AAGAAAGCTT CAACACTATC 
AGCAACATTG AGGAGATAGC TTATAACGCC ATTTCCTTCA CGTGGGACAT CAATGATGAG 
GCAAAGGTCT TCATCTCTGT GAACTGCTTG AGCACAGATT TCTCTTCTCA GAAGGGTGTG 
AAGGGCTTGC CACTCAACAT TCAAATCGAC ACATACAGCT ATAACAACCG CAGCAACAAG 
CCGGTTCACC GGGCCTACTG CCAGATAAAG GTCTTCTGCG ACAAGGGAGC TGAAAGGAAA 
ATTCGGGATG AAGAACGAAA ACAGAGCAAG AGAAAAGTGT CTGACGTTAA AGTGCAGCTG 
CTTCCCTCGC ACAAACGGAC AGACATCACA GTGTTCAAGC CCTTCCTGGA CCTCGACACT 
CAGCCTGTCC TCTTCATTCC GGACGTGCAT TTTACCAACC TGCAGCGGGG CAGTCATGTT 
CTTTCCCTCC CCTCTGAAGA ACTGGAAGGT GAAGGCTCTG TCTTGAAAAG AGGGCCATTC 
GGAACCGAAG ATGACTTTGG AGTTCCTCCT CCTGCTAAGC TGACTCGGAC AGAAGAACCC 
AAGAGAGTGC TGCTCTATGT CCGAAAGGAA TCAGAAGAAG TCTTCGACGC CCTGATGCTC 
AAGACGCCGT CTTTGAAGGG CCTGATGGAG GCAATTTCAG ACAAGTATGA TGTCCCCCAT 
GACAAGATTG GGAAAATATT TAAGAAGTGC AAAAAAGGGA TCCTCGTGAA CATGGACGAC 
AACATTGTGA AGCACTACTC CAATGAGGAC ACCTTCCAGC TGCAGATAGA GGAAGCCGGC 
GGCTCGTACA AGCTCACCCT GACAGAGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grhl1 ( XM_030246623.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102483.1 Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu78177
Clone ID Related Accession (Same CDS sequence)	XM_030246622.1 , XM_030246623.1 , XM_017315004.1
Accession Version	XM_030246623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	grainyhead-like protein 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCG GAGGACTCCA GACTCCACCT TCTCCGAGAC CTTCAAGGAG GGCGTTCAGG 
AGGAACAACT TTGAGTATAC CCTGGAAGCC TCCAAGTCAC TGCGGCAGAA GCAAGGGGAC 
AGCACTATGA CATACCTGAA TAAAGGCCAG TTCTATCCTG TCACCTTAAA GGAAGGAAGC 
AGCAATGAAG GGATTCACCA CCCTATCAGC AAAGTTCGAA GTGTGATCAT GGTGGTTTTT 
GCTGAAGACA AAAGCAGAGA AGACCAGCTG AGACACTGGA AGTACTGGCA CTCCCGTCAG 
CACACGGCCA AACAGAGGTG CATTGACATT GCTGACTACA AAGAAAGCTT CAACACTATC 
AGCAACATTG AGGAGATAGC TTATAACGCC ATTTCCTTCA CGTGGGACAT CAATGATGAG 
GCAAAGGTCT TCATCTCTGT GAACTGCTTG AGCACAGATT TCTCTTCTCA GAAGGGTGTG 
AAGGGCTTGC CACTCAACAT TCAAATCGAC ACATACAGCT ATAACAACCG CAGCAACAAG 
CCGGTTCACC GGGCCTACTG CCAGATAAAG GTCTTCTGCG ACAAGGGAGC TGAAAGGAAA 
ATTCGGGATG AAGAACGAAA ACAGAGCAAG AGAAAAGTGT CTGACGTTAA AGTGCAGCTG 
CTTCCCTCGC ACAAACGGAC AGACATCACA GTGTTCAAGC CCTTCCTGGA CCTCGACACT 
CAGCCTGTCC TCTTCATTCC GGACGTGCAT TTTACCAACC TGCAGCGGGG CAGTCATGTT 
CTTTCCCTCC CCTCTGAAGA ACTGGAAGGT GAAGGCTCTG TCTTGAAAAG AGGGCCATTC 
GGAACCGAAG ATGACTTTGG AGTTCCTCCT CCTGCTAAGC TGACTCGGAC AGAAGAACCC 
AAGAGAGTGC TGCTCTATGT CCGAAAGGAA TCAGAAGAAG TCTTCGACGC CCTGATGCTC 
AAGACGCCGT CTTTGAAGGG CCTGATGGAG GCAATTTCAG ACAAGTATGA TGTCCCCCAT 
GACAAGATTG GGAAAATATT TAAGAAGTGC AAAAAAGGGA TCCTCGTGAA CATGGACGAC 
AACATTGTGA AGCACTACTC CAATGAGGAC ACCTTCCAGC TGCAGATAGA GGAAGCCGGC 
GGCTCGTACA AGCTCACCCT GACAGAGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102483.1
CDS	809..1981
Translation	MLSGGLQTPPSPRPSRRAFRRNNFEYTLEASKSLRQKQGDSTMTYLNKGQFYPVTLKEGSSNEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDEERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLSLPSEELEGEGSVLKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI

Target ORF information:

RefSeq Version	XM_030246623.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246623.1

ORF Insert Sequence:

ATGCTCAGCG GAGGACTCCA GACTCCACCT TCTCCGAGAC CTTCAAGGAG GGCGTTCAGG 
AGGAACAACT TTGAGTATAC CCTGGAAGCC TCCAAGTCAC TGCGGCAGAA GCAAGGGGAC 
AGCACTATGA CATACCTGAA TAAAGGCCAG TTCTATCCTG TCACCTTAAA GGAAGGAAGC 
AGCAATGAAG GGATTCACCA CCCTATCAGC AAAGTTCGAA GTGTGATCAT GGTGGTTTTT 
GCTGAAGACA AAAGCAGAGA AGACCAGCTG AGACACTGGA AGTACTGGCA CTCCCGTCAG 
CACACGGCCA AACAGAGGTG CATTGACATT GCTGACTACA AAGAAAGCTT CAACACTATC 
AGCAACATTG AGGAGATAGC TTATAACGCC ATTTCCTTCA CGTGGGACAT CAATGATGAG 
GCAAAGGTCT TCATCTCTGT GAACTGCTTG AGCACAGATT TCTCTTCTCA GAAGGGTGTG 
AAGGGCTTGC CACTCAACAT TCAAATCGAC ACATACAGCT ATAACAACCG CAGCAACAAG 
CCGGTTCACC GGGCCTACTG CCAGATAAAG GTCTTCTGCG ACAAGGGAGC TGAAAGGAAA 
ATTCGGGATG AAGAACGAAA ACAGAGCAAG AGAAAAGTGT CTGACGTTAA AGTGCAGCTG 
CTTCCCTCGC ACAAACGGAC AGACATCACA GTGTTCAAGC CCTTCCTGGA CCTCGACACT 
CAGCCTGTCC TCTTCATTCC GGACGTGCAT TTTACCAACC TGCAGCGGGG CAGTCATGTT 
CTTTCCCTCC CCTCTGAAGA ACTGGAAGGT GAAGGCTCTG TCTTGAAAAG AGGGCCATTC 
GGAACCGAAG ATGACTTTGG AGTTCCTCCT CCTGCTAAGC TGACTCGGAC AGAAGAACCC 
AAGAGAGTGC TGCTCTATGT CCGAAAGGAA TCAGAAGAAG TCTTCGACGC CCTGATGCTC 
AAGACGCCGT CTTTGAAGGG CCTGATGGAG GCAATTTCAG ACAAGTATGA TGTCCCCCAT 
GACAAGATTG GGAAAATATT TAAGAAGTGC AAAAAAGGGA TCCTCGTGAA CATGGACGAC 
AACATTGTGA AGCACTACTC CAATGAGGAC ACCTTCCAGC TGCAGATAGA GGAAGCCGGC 
GGCTCGTACA AGCTCACCCT GACAGAGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grhl1 ( XM_017315004.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170493.1 Mus musculus grainyhead-like 1 (Drosophila) (Grhl1), transcript variant X3, mRNA.

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CloneID	OMu78177
Clone ID Related Accession (Same CDS sequence)	XM_030246622.1 , XM_030246623.1 , XM_017315004.1
Accession Version	XM_017315004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	grainyhead-like protein 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCG GAGGACTCCA GACTCCACCT TCTCCGAGAC CTTCAAGGAG GGCGTTCAGG 
AGGAACAACT TTGAGTATAC CCTGGAAGCC TCCAAGTCAC TGCGGCAGAA GCAAGGGGAC 
AGCACTATGA CATACCTGAA TAAAGGCCAG TTCTATCCTG TCACCTTAAA GGAAGGAAGC 
AGCAATGAAG GGATTCACCA CCCTATCAGC AAAGTTCGAA GTGTGATCAT GGTGGTTTTT 
GCTGAAGACA AAAGCAGAGA AGACCAGCTG AGACACTGGA AGTACTGGCA CTCCCGTCAG 
CACACGGCCA AACAGAGGTG CATTGACATT GCTGACTACA AAGAAAGCTT CAACACTATC 
AGCAACATTG AGGAGATAGC TTATAACGCC ATTTCCTTCA CGTGGGACAT CAATGATGAG 
GCAAAGGTCT TCATCTCTGT GAACTGCTTG AGCACAGATT TCTCTTCTCA GAAGGGTGTG 
AAGGGCTTGC CACTCAACAT TCAAATCGAC ACATACAGCT ATAACAACCG CAGCAACAAG 
CCGGTTCACC GGGCCTACTG CCAGATAAAG GTCTTCTGCG ACAAGGGAGC TGAAAGGAAA 
ATTCGGGATG AAGAACGAAA ACAGAGCAAG AGAAAAGTGT CTGACGTTAA AGTGCAGCTG 
CTTCCCTCGC ACAAACGGAC AGACATCACA GTGTTCAAGC CCTTCCTGGA CCTCGACACT 
CAGCCTGTCC TCTTCATTCC GGACGTGCAT TTTACCAACC TGCAGCGGGG CAGTCATGTT 
CTTTCCCTCC CCTCTGAAGA ACTGGAAGGT GAAGGCTCTG TCTTGAAAAG AGGGCCATTC 
GGAACCGAAG ATGACTTTGG AGTTCCTCCT CCTGCTAAGC TGACTCGGAC AGAAGAACCC 
AAGAGAGTGC TGCTCTATGT CCGAAAGGAA TCAGAAGAAG TCTTCGACGC CCTGATGCTC 
AAGACGCCGT CTTTGAAGGG CCTGATGGAG GCAATTTCAG ACAAGTATGA TGTCCCCCAT 
GACAAGATTG GGAAAATATT TAAGAAGTGC AAAAAAGGGA TCCTCGTGAA CATGGACGAC 
AACATTGTGA AGCACTACTC CAATGAGGAC ACCTTCCAGC TGCAGATAGA GGAAGCCGGC 
GGCTCGTACA AGCTCACCCT GACAGAGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170493.1
CDS	810..1982
Translation	MLSGGLQTPPSPRPSRRAFRRNNFEYTLEASKSLRQKQGDSTMTYLNKGQFYPVTLKEGSSNEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDEERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLSLPSEELEGEGSVLKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI

Target ORF information:

RefSeq Version	XM_017315004.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus grainyhead-like 1 (Drosophila) (Grhl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315004.1

ORF Insert Sequence:

ATGCTCAGCG GAGGACTCCA GACTCCACCT TCTCCGAGAC CTTCAAGGAG GGCGTTCAGG 
AGGAACAACT TTGAGTATAC CCTGGAAGCC TCCAAGTCAC TGCGGCAGAA GCAAGGGGAC 
AGCACTATGA CATACCTGAA TAAAGGCCAG TTCTATCCTG TCACCTTAAA GGAAGGAAGC 
AGCAATGAAG GGATTCACCA CCCTATCAGC AAAGTTCGAA GTGTGATCAT GGTGGTTTTT 
GCTGAAGACA AAAGCAGAGA AGACCAGCTG AGACACTGGA AGTACTGGCA CTCCCGTCAG 
CACACGGCCA AACAGAGGTG CATTGACATT GCTGACTACA AAGAAAGCTT CAACACTATC 
AGCAACATTG AGGAGATAGC TTATAACGCC ATTTCCTTCA CGTGGGACAT CAATGATGAG 
GCAAAGGTCT TCATCTCTGT GAACTGCTTG AGCACAGATT TCTCTTCTCA GAAGGGTGTG 
AAGGGCTTGC CACTCAACAT TCAAATCGAC ACATACAGCT ATAACAACCG CAGCAACAAG 
CCGGTTCACC GGGCCTACTG CCAGATAAAG GTCTTCTGCG ACAAGGGAGC TGAAAGGAAA 
ATTCGGGATG AAGAACGAAA ACAGAGCAAG AGAAAAGTGT CTGACGTTAA AGTGCAGCTG 
CTTCCCTCGC ACAAACGGAC AGACATCACA GTGTTCAAGC CCTTCCTGGA CCTCGACACT 
CAGCCTGTCC TCTTCATTCC GGACGTGCAT TTTACCAACC TGCAGCGGGG CAGTCATGTT 
CTTTCCCTCC CCTCTGAAGA ACTGGAAGGT GAAGGCTCTG TCTTGAAAAG AGGGCCATTC 
GGAACCGAAG ATGACTTTGG AGTTCCTCCT CCTGCTAAGC TGACTCGGAC AGAAGAACCC 
AAGAGAGTGC TGCTCTATGT CCGAAAGGAA TCAGAAGAAG TCTTCGACGC CCTGATGCTC 
AAGACGCCGT CTTTGAAGGG CCTGATGGAG GCAATTTCAG ACAAGTATGA TGTCCCCCAT 
GACAAGATTG GGAAAATATT TAAGAAGTGC AAAAAAGGGA TCCTCGTGAA CATGGACGAC 
AACATTGTGA AGCACTACTC CAATGAGGAC ACCTTCCAGC TGCAGATAGA GGAAGCCGGC 
GGCTCGTACA AGCTCACCCT GACAGAGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grhl1 ( XM_006515016.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515079.1 Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X1, mRNA.

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CloneID	OMu47263
Clone ID Related Accession (Same CDS sequence)	XM_006515016.3 , XM_006515016.4
Accession Version	XM_006515016.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	grainyhead-like protein 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515016.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACAGG AGTACGACAA CAAAAGGCCC GTGCTGGTAC TTCAGAATGA AGCCCTCTAC 
CCACAGCGGC GCTCCTATAC CAGTGAGGAT GAAGCCTGGA AGTCGTTCCT GGAAAACCCT 
CTCACTGCGG CAACCAAAGC GATGATGAGC ATCAACGGAG ACGAAGACAG CGCGGCTGCG 
CTGGGCCTGC TCTATGACTA CTACAAGGTC CCCAGAGAGC GCCGGTCATC AGCCGTAAAG 
CCCGAGGGAG AGCACCCAGA GCCAGAGCAC AGCAAAAGCA TACCAAATGT GACGGAGCAG 
CCCCTCATTT CTGCTGGAGA AAACAGGGTG CAAGTGCTGA AAAACGTGCC CTTCAACATC 
GTCCTCCCCC ATAGCAACCA GCTGGGCATT GATAAGAGAG GCCATCTGAC AGCTCCCGAT 
ACAACAGTCA CTGTCTCCAT AGCAACCATG CCTACCCACT CCATCAAGAC AGAAATCCAG 
CCGCACGGCT TTGCTGTGGG AATCCCACCA GCCGTGTACC ACTCTGAGCC CACCGAACGT 
GTGGTGGTTT TTGACCGGAG CCTCAGCACT GATCAGTTCA GCTCTGGCAC TCAGCCCCCC 
AATGCTCAGC GGAGGACTCC AGACTCCACC TTCTCCGAGA CCTTCAAGGA GGGCGTTCAG 
GAGGTTTTCT TCCCCTCGGA ACTCAGCCTT CGGATGCCGG GCATGAATTC AGAGGACTAT 
GTCTTTGACA ATGTTTCTGG GAACAACTTT GAGTATACCC TGGAAGCCTC CAAGTCACTG 
CGGCAGAAGC AAGGGGACAG CACTATGACA TACCTGAATA AAGGCCAGTT CTATCCTGTC 
ACCTTAAAGG AAGGAAGCAG CAATGAAGGG ATTCACCACC CTATCAGCAA AGTTCGAAGT 
GTGATCATGG TGGTTTTTGC TGAAGACAAA AGCAGAGAAG ACCAGCTGAG ACACTGGAAG 
TACTGGCACT CCCGTCAGCA CACGGCCAAA CAGAGGTGCA TTGACATTGC TGACTACAAA 
GAAAGCTTCA ACACTATCAG CAACATTGAG GAGATAGCTT ATAACGCCAT TTCCTTCACG 
TGGGACATCA ATGATGAGGC AAAGGTCTTC ATCTCTGTGA ACTGCTTGAG CACAGATTTC 
TCTTCTCAGA AGGGTGTGAA GGGCTTGCCA CTCAACATTC AAATCGACAC ATACAGCTAT 
AACAACCGCA GCAACAAGCC GGTTCACCGG GCCTACTGCC AGATAAAGGT CTTCTGCGAC 
AAGGGAGCTG AAAGGAAAAT TCGGGATGAA GAACGAAAAC AGAGCAAGAG AAAAGTGTCT 
GACGTTAAAG TGCAGCTGCT TCCCTCGCAC AAACGGACAG ACATCACAGT GTTCAAGCCC 
TTCCTGGACC TCGACACTCA GCCTGTCCTC TTCATTCCGG ACGTGCATTT TACCAACCTG 
CAGCGGGGCA GTCATGTTCT TTCCCTCCCC TCTGAAGAAC TGGAAGGTGA AGGCTCTGTC 
TTGAAAAGAG GGCCATTCGG AACCGAAGAT GACTTTGGAG TTCCTCCTCC TGCTAAGCTG 
ACTCGGACAG AAGAACCCAA GAGAGTGCTG CTCTATGTCC GAAAGGAATC AGAAGAAGTC 
TTCGACGCCC TGATGCTCAA GACGCCGTCT TTGAAGGGCC TGATGGAGGC AATTTCAGAC 
AAGTATGATG TCCCCCATGA CAAGATTGGG AAAATATTTA AGAAGTGCAA AAAAGGGATC 
CTCGTGAACA TGGACGACAA CATTGTGAAG CACTACTCCA ATGAGGACAC CTTCCAGCTG 
CAGATAGAGG AAGCCGGCGG CTCGTACAAG CTCACCCTGA CAGAGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515079.1
CDS	208..2058
Translation	MTQEYDNKRPVLVLQNEALYPQRRSYTSEDEAWKSFLENPLTAATKAMMSINGDEDSAAALGLLYDYYKVPRERRSSAVKPEGEHPEPEHSKSIPNVTEQPLISAGENRVQVLKNVPFNIVLPHSNQLGIDKRGHLTAPDTTVTVSIATMPTHSIKTEIQPHGFAVGIPPAVYHSEPTERVVVFDRSLSTDQFSSGTQPPNAQRRTPDSTFSETFKEGVQEVFFPSELSLRMPGMNSEDYVFDNVSGNNFEYTLEASKSLRQKQGDSTMTYLNKGQFYPVTLKEGSSNEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDEERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLSLPSEELEGEGSVLKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI

Target ORF information:

RefSeq Version	XM_006515016.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus grainyhead like transcription factor 1 (Grhl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515016.4

ORF Insert Sequence:

ATGACACAGG AGTACGACAA CAAAAGGCCC GTGCTGGTAC TTCAGAATGA AGCCCTCTAC 
CCACAGCGGC GCTCCTATAC CAGTGAGGAT GAAGCCTGGA AGTCGTTCCT GGAAAACCCT 
CTCACTGCGG CAACCAAAGC GATGATGAGC ATCAACGGAG ACGAAGACAG CGCGGCTGCG 
CTGGGCCTGC TCTATGACTA CTACAAGGTC CCCAGAGAGC GCCGGTCATC AGCCGTAAAG 
CCCGAGGGAG AGCACCCAGA GCCAGAGCAC AGCAAAAGCA TACCAAATGT GACGGAGCAG 
CCCCTCATTT CTGCTGGAGA AAACAGGGTG CAAGTGCTGA AAAACGTGCC CTTCAACATC 
GTCCTCCCCC ATAGCAACCA GCTGGGCATT GATAAGAGAG GCCATCTGAC AGCTCCCGAT 
ACAACAGTCA CTGTCTCCAT AGCAACCATG CCTACCCACT CCATCAAGAC AGAAATCCAG 
CCGCACGGCT TTGCTGTGGG AATCCCACCA GCCGTGTACC ACTCTGAGCC CACCGAACGT 
GTGGTGGTTT TTGACCGGAG CCTCAGCACT GATCAGTTCA GCTCTGGCAC TCAGCCCCCC 
AATGCTCAGC GGAGGACTCC AGACTCCACC TTCTCCGAGA CCTTCAAGGA GGGCGTTCAG 
GAGGTTTTCT TCCCCTCGGA ACTCAGCCTT CGGATGCCGG GCATGAATTC AGAGGACTAT 
GTCTTTGACA ATGTTTCTGG GAACAACTTT GAGTATACCC TGGAAGCCTC CAAGTCACTG 
CGGCAGAAGC AAGGGGACAG CACTATGACA TACCTGAATA AAGGCCAGTT CTATCCTGTC 
ACCTTAAAGG AAGGAAGCAG CAATGAAGGG ATTCACCACC CTATCAGCAA AGTTCGAAGT 
GTGATCATGG TGGTTTTTGC TGAAGACAAA AGCAGAGAAG ACCAGCTGAG ACACTGGAAG 
TACTGGCACT CCCGTCAGCA CACGGCCAAA CAGAGGTGCA TTGACATTGC TGACTACAAA 
GAAAGCTTCA ACACTATCAG CAACATTGAG GAGATAGCTT ATAACGCCAT TTCCTTCACG 
TGGGACATCA ATGATGAGGC AAAGGTCTTC ATCTCTGTGA ACTGCTTGAG CACAGATTTC 
TCTTCTCAGA AGGGTGTGAA GGGCTTGCCA CTCAACATTC AAATCGACAC ATACAGCTAT 
AACAACCGCA GCAACAAGCC GGTTCACCGG GCCTACTGCC AGATAAAGGT CTTCTGCGAC 
AAGGGAGCTG AAAGGAAAAT TCGGGATGAA GAACGAAAAC AGAGCAAGAG AAAAGTGTCT 
GACGTTAAAG TGCAGCTGCT TCCCTCGCAC AAACGGACAG ACATCACAGT GTTCAAGCCC 
TTCCTGGACC TCGACACTCA GCCTGTCCTC TTCATTCCGG ACGTGCATTT TACCAACCTG 
CAGCGGGGCA GTCATGTTCT TTCCCTCCCC TCTGAAGAAC TGGAAGGTGA AGGCTCTGTC 
TTGAAAAGAG GGCCATTCGG AACCGAAGAT GACTTTGGAG TTCCTCCTCC TGCTAAGCTG 
ACTCGGACAG AAGAACCCAA GAGAGTGCTG CTCTATGTCC GAAAGGAATC AGAAGAAGTC 
TTCGACGCCC TGATGCTCAA GACGCCGTCT TTGAAGGGCC TGATGGAGGC AATTTCAGAC 
AAGTATGATG TCCCCCATGA CAAGATTGGG AAAATATTTA AGAAGTGCAA AAAAGGGATC 
CTCGTGAACA TGGACGACAA CATTGTGAAG CACTACTCCA ATGAGGACAC CTTCCAGCTG 
CAGATAGAGG AAGCCGGCGG CTCGTACAAG CTCACCCTGA CAGAGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Grainyhead-like 3 (Grhl3) deficiency in brain leads to altered locomotor activity and decreased anxiety-like behaviors in aged mice.
Developmental neurobiology77(6)775-788(2017 Jun)
Dworkin S,Auden A,Partridge DD,Daglas M,Medcalf RL,Mantamadiotis T,Georgy SR,Darido C,Jane SM,Ting SB

Effects of high and low sodium diet on blood pressure and heart rate in mice lacking the functional grainyhead-like 1 gene.
Physiological research66(1)163-165(2017 Mar)
Walkowska A,Pawlak M,Jane SM,Kompanowska-Jezierska E,Wilanowski T

Two Ancient Gene Families Are Critical for Maintenance of the Mammalian Skin Barrier in Postnatal Life.
The Journal of investigative dermatology136(7)1438-1448(2016 Jul)
Cangkrama M,Darido C,Georgy SR,Partridge D,Auden A,Srivastava S,Wilanowski T,Jane SM

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Grhl1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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