Rad21 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rad21
Entrez Gene ID	19357
Full Name	RAD21 cohesin complex component
Synonyms	SCC1,mKIAA0078
General protein information	Preferred Names RAD21 cohesin complex component Names double-strand-break repair protein rad21 homolog PW29 RAD21 homolog SCC1 homolog pokeweed agglutinin-binding protein 29
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_009009.4	NP_033035.3	double-strand-break repair protein rad21 homolog
XM_006520644.1	XP_006520707.1	double-strand-break repair protein rad21 homolog isoform X1
XM_006520645.2	XP_006520708.1	double-strand-break repair protein rad21 homolog isoform X1
XM_006520645.1	XP_006520708.1	double-strand-break repair protein rad21 homolog isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAD21	NP_006256.1
	RAD21	XP_001095142.1
Bos taurus (cattle)	RAD21	NP_001029889.1
Drosophila melanogaster (fruit fly)	vtd	NP_001015132.1
Canis lupus familiaris (dog)	RAD21	XP_539142.2
Mus musculus (house mouse)	Rad21	NP_033035.3
Gallus gallus (chicken)	RAD21	NP_001026121.1
Danio rerio (zebrafish)	LOC100536176	XP_003200317.1
Rattus norvegicus (Norway rat)	Rad21	NP_001020872.1
Danio rerio (zebrafish)	rad21b	NP_001038585.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004560	XP_313862.4
Xenopus tropicalis (tropical clawed frog)	rad21	XP_002940047.1
Pan troglodytes (chimpanzee)	RAD21	XP_003951384.1
Danio rerio (zebrafish)	rad21a	NP_955889.1

Gene Ontology
Bibliography

Related articles in PubMed

A mechanism of cohesin-dependent loop extrusion organizes zygotic genome architecture.
Gassler J, Brand?o HB, Imakaev M, Flyamer IM, Ladst?tter S, Bickmore WA, Peters JM, Mirny LA, Tachibana K
The EMBO journal36(24)3600-3618(2017 12)

5C analysis of the Epidermal Differentiation Complex locus reveals distinct chromatin interaction networks between gene-rich and gene-poor TADs in skin epithelial cells.
Poterlowicz K, Yarker JL, Malashchuk I, Lajoie BR, Mardaryev AN, Gdula MR, Sharov AA, Kohwi-Shigematsu T, Botchkarev VA, Fessing MY
PLoS genetics13(9)e1006966(2017 Sep)

The cohesin subunit Rad21 is a negative regulator of hematopoietic self-renewal through epigenetic repression of Hoxa7 and Hoxa9.
Fisher JB, Peterson J, Reimer M, Stelloh C, Pulakanti K, Gerbec ZJ, Abel AM, Strouse JM, Strouse C, McNulty M, Malarkannan S, Crispino JD, Milanovich S, Rao S
Leukemia31(3)712-719(2017 Mar)

A Surveillance Mechanism Ensures Repair of DNA Lesions during Zygotic Reprogramming.
Ladst?tter S, Tachibana-Konwalski K
Cell167(7)1774-1787.e13(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results demonstrate that the zygotic genome folds into loops and domains that critically depend on Scc1-cohesin and that are regulated in size and linear density by Wapl.
Title: A mechanism of cohesin-dependent loop extrusion organizes zygotic genome architecture.

n keratinocytes, the promoter-enhancer anchoring regions in the gene-rich transcriptionally active TADs are enriched for the binding of chromatin architectural proteins CTCF, Rad21 and chromatin remodeler Brg1. In contrast to gene-rich TADs, gene-poor TADs show preferential spatial contacts with each other, do not contain active enhancers and show decreased binding of CTCF, Rad21 and Brg1 in keratinocytes
Title: 5C analysis of the Epidermal Differentiation Complex locus reveals distinct chromatin interaction networks between gene-rich and gene-poor TADs in skin epithelial cells.

the cohesin complex regulates PRC2 targeting to silence Hoxa7 and Hoxa9 and negatively regulate self-renewal. Our studies identify a novel epigenetic mechanism underlying leukemogenesis in AML patients with cohesin mutations.
Title: The cohesin subunit Rad21 is a negative regulator of hematopoietic self-renewal through epigenetic repression of Hoxa7 and Hoxa9.

Knockdown of Rad21 reduces expression of the constitutive, biallelically expressed Pcdhalpha isoforms alphac1 and alphac2.
Title: Role of CCCTC binding factor (CTCF) and cohesin in the generation of single-cell diversity of protocadherin-? gene expression.

show that spermatocytes from mice lacking the two meiosis-specific cohesin subunits RAD21L and REC8 were unable to initiate RAD51- but not DMC1-mediated double-strand break repair
Title: Meiotic cohesin complexes are essential for the formation of the axial element in mice.

The following Rad21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rad21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03314	NM_009009.4 Latest version!	Mus musculus RAD21 cohesin complex component (Rad21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu03314	XM_006520644.1 Latest version!	Mus musculus RAD21 cohesin complex component (Rad21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu03314	XM_006520645.2 Latest version!	Mus musculus RAD21 cohesin complex component (Rad21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu03314	XM_006520645.1	Mus musculus RAD21 cohesin complex component (Rad21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rad21 ( NM_009009.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033035.3 Mus musculus RAD21 cohesin complex component (Rad21), mRNA.

Price & Availability↑

CloneID	OMu03314
Clone ID Related Accession (Same CDS sequence)	NM_009009.4 , XM_006520644.1 , XM_006520645.2 , XM_006520645.1
Accession Version	NM_009009.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Mus musculus(house mouse)
Product	double-strand-break repair protein rad21 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ072039.1, AK168718.1, AK163992.1 and BC043032.1. On Jul 25, 2009 this sequence version replaced NM_009009.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK168718.1, AK145591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTACG CACATTTTGT CCTCAGTAAA CGAGGGCCTC TGGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAGAAGCT AACCAAAGCC CATGTATTTG AGTGCAACTT AGAGAGCAGT 
GTGGAGAGCA TCATCTCACC AAAGGTGAAG ATGGCGCTGC GGACGTCAGG ACACCTTCTC 
CTGGGAGTAG TCCGCATCTA TCACAGGAAA GCCAAATACC TCCTCGCAGA CTGTAATGAA 
GCATTTATTA AAATAAAGAT GGCGTTTCGG CCAGGTGTTG TCGATCTACC TGAGGAAAAT 
CGGGAAGCAG CTTATAATGC CATTACTTTA CCTGAGGAAT TCCACGATTT TGATCAGCCA 
CTGCCAGATT TAGATGATAT TGACGTCGCC CAGCAGTTCA GCCTGAACCA AAGCAGAGTA 
GAAGAGATAA CCATGAGAGA AGAAGTCGGA AACATCAGTA TCCTACAGGA AAATGACTTT 
GGTGACTTCG GAATGGATGA CCGTGAAATA ATGAGAGAAG GCAGTGCTTT CGAGGATGAC 
GACATGTTAG TGAGCACCAG CGCTTCCAAC CTTCTCCTCG AGCCAGAGCA GAGCACCAGC 
AACCTGAATG AAAAGATGAA TCACTTAGAG TACGAAGACC AGTACAAAGA TGACAATTTT 
GGAGAAGGAA ATGATGGCGG TATATTAGAT GACAAACTTA TAAGTAATAA TGATGGTGGC 
ATCTTTGACG ATCCCCCTGC CTTGTCTGAG GCAGGGGTCA TGTTGCCAGA GCAACCTGCA 
CATGATGACA TGGATGAAGA TGACAATGGC TCACTGGGTG GGCCGGATAG TCCCGACTCT 
GTGGATCCTG TCGAACCGAT GCCAACTATG ACTGATCAGA CAACTCTCGT CCCAAACGAG 
GAAGAAGCTT TTGCGTTGGA GCCCATTGAT ATAACTGTCA AAGAGACAAA AGCCAAGAGG 
AAGAGGAAGC TGATTGTTGA CAGTGTCAAA GAATTGGATA GTAAGACCAT TAGAGCCCAG 
CTTAGCGATT ATTCTGATAT TGTTACGACT CTGGACCTGG CTCCGCCAAC CAAGAAGCTT 
ATGATGTGGA AAGAGACAGG AGGAGTGGAA AAGCTCTTCT TTTTACCAGC ACAGCCCCTG 
TGGAATAACC GGCTACTGAA GCTCTTCACA CGCTGCCTTA CCCCACTTGT ACCAGAAGAC 
CTTAGGAAGA GAAGGAAAGG GGGAGAGGCA GATAATCTGG ATGAGTTCCT CAAAGAGTTT 
GAGAATCCAG AGGTTCCCAG AGAGGAGCAG CAGCCACAGC AGCAGCAGCC ACAGCCGCAG 
CGAGATGTCA TCGATGAGCC CATTATAGAA GAGCCAAGCC GCCTCCAGGA CTCAGTGATG 
GAGGCCAGCA GAACAACCAT AGAAGAATCA GCCATGCCCC CACCACCCCC TCAAGGAGTT 
AAGCGGAAAG CCGGGCAAAT AGACCCAGAG CCTTCGATAC CTCCTCAGCA GGTAGAGCAA 
ATGGAAATAC CACCAGTAGA ACTTCCCCCA GAGGAGCCTC CAAATATCTG TCAGCTGATC 
CCGGAGTTAG AGCTCCTACC GGAGAAGGAG AAGGAAAAAG AGAAGGAGAA GGAAGAGGAG 
GAGGAGGAGG AGGATGAAGA TGCTTCAGGG GGTGATCAGG ATCAAGAGGA AAGGAGATGG 
AACAAACGCA CTCAGCAGAT GCTTCATGGT CTTCAGCGAG CTCTTGCTAA AACTGGAGCA 
GAGTCTATCA GTTTGCTTGA GCTGTGTCGA AACACAAACC GAAAGCAGGC AGCAGCAAAG 
TTCTACAGCT TTTTGGTTCT TAAGAAGCAG CAAGCCATCG AGCTCACACA GGAAGAGCCG 
TACAGTGACA TCATTGCAAC CCCTGGACCA CGGTTCCATA TTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033035.3
CDS	246..2153
Translation	MFYAHFVLSKRGPLAKIWLAAHWDKKLTKAHVFECNLESSVESIISPKVKMALRTSGHLLLGVVRIYHRKAKYLLADCNEAFIKIKMAFRPGVVDLPEENREAAYNAITLPEEFHDFDQPLPDLDDIDVAQQFSLNQSRVEEITMREEVGNISILQENDFGDFGMDDREIMREGSAFEDDDMLVSTSASNLLLEPEQSTSNLNEKMNHLEYEDQYKDDNFGEGNDGGILDDKLISNNDGGIFDDPPALSEAGVMLPEQPAHDDMDEDDNGSLGGPDSPDSVDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVTTLDLAPPTKKLMMWKETGGVEKLFFLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEFENPEVPREEQQPQQQQPQPQRDVIDEPIIEEPSRLQDSVMEASRTTIEESAMPPPPPQGVKRKAGQIDPEPSIPPQQVEQMEIPPVELPPEEPPNICQLIPELELLPEKEKEKEKEKEEEEEEEDEDASGGDQDQEERRWNKRTQQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGPRFHII

Target ORF information:

RefSeq Version	NM_009009.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD21 cohesin complex component (Rad21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009009.4

ORF Insert Sequence:

ATGTTCTACG CACATTTTGT CCTCAGTAAA CGAGGGCCTC TGGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAGAAGCT AACCAAAGCC CATGTATTTG AGTGCAACTT AGAGAGCAGT 
GTGGAGAGCA TCATCTCACC AAAGGTGAAG ATGGCGCTGC GGACGTCAGG ACACCTTCTC 
CTGGGAGTAG TCCGCATCTA TCACAGGAAA GCCAAATACC TCCTCGCAGA CTGTAATGAA 
GCATTTATTA AAATAAAGAT GGCGTTTCGG CCAGGTGTTG TCGATCTACC TGAGGAAAAT 
CGGGAAGCAG CTTATAATGC CATTACTTTA CCTGAGGAAT TCCACGATTT TGATCAGCCA 
CTGCCAGATT TAGATGATAT TGACGTCGCC CAGCAGTTCA GCCTGAACCA AAGCAGAGTA 
GAAGAGATAA CCATGAGAGA AGAAGTCGGA AACATCAGTA TCCTACAGGA AAATGACTTT 
GGTGACTTCG GAATGGATGA CCGTGAAATA ATGAGAGAAG GCAGTGCTTT CGAGGATGAC 
GACATGTTAG TGAGCACCAG CGCTTCCAAC CTTCTCCTCG AGCCAGAGCA GAGCACCAGC 
AACCTGAATG AAAAGATGAA TCACTTAGAG TACGAAGACC AGTACAAAGA TGACAATTTT 
GGAGAAGGAA ATGATGGCGG TATATTAGAT GACAAACTTA TAAGTAATAA TGATGGTGGC 
ATCTTTGACG ATCCCCCTGC CTTGTCTGAG GCAGGGGTCA TGTTGCCAGA GCAACCTGCA 
CATGATGACA TGGATGAAGA TGACAATGGC TCACTGGGTG GGCCGGATAG TCCCGACTCT 
GTGGATCCTG TCGAACCGAT GCCAACTATG ACTGATCAGA CAACTCTCGT CCCAAACGAG 
GAAGAAGCTT TTGCGTTGGA GCCCATTGAT ATAACTGTCA AAGAGACAAA AGCCAAGAGG 
AAGAGGAAGC TGATTGTTGA CAGTGTCAAA GAATTGGATA GTAAGACCAT TAGAGCCCAG 
CTTAGCGATT ATTCTGATAT TGTTACGACT CTGGACCTGG CTCCGCCAAC CAAGAAGCTT 
ATGATGTGGA AAGAGACAGG AGGAGTGGAA AAGCTCTTCT TTTTACCAGC ACAGCCCCTG 
TGGAATAACC GGCTACTGAA GCTCTTCACA CGCTGCCTTA CCCCACTTGT ACCAGAAGAC 
CTTAGGAAGA GAAGGAAAGG GGGAGAGGCA GATAATCTGG ATGAGTTCCT CAAAGAGTTT 
GAGAATCCAG AGGTTCCCAG AGAGGAGCAG CAGCCACAGC AGCAGCAGCC ACAGCCGCAG 
CGAGATGTCA TCGATGAGCC CATTATAGAA GAGCCAAGCC GCCTCCAGGA CTCAGTGATG 
GAGGCCAGCA GAACAACCAT AGAAGAATCA GCCATGCCCC CACCACCCCC TCAAGGAGTT 
AAGCGGAAAG CCGGGCAAAT AGACCCAGAG CCTTCGATAC CTCCTCAGCA GGTAGAGCAA 
ATGGAAATAC CACCAGTAGA ACTTCCCCCA GAGGAGCCTC CAAATATCTG TCAGCTGATC 
CCGGAGTTAG AGCTCCTACC GGAGAAGGAG AAGGAAAAAG AGAAGGAGAA GGAAGAGGAG 
GAGGAGGAGG AGGATGAAGA TGCTTCAGGG GGTGATCAGG ATCAAGAGGA AAGGAGATGG 
AACAAACGCA CTCAGCAGAT GCTTCATGGT CTTCAGCGAG CTCTTGCTAA AACTGGAGCA 
GAGTCTATCA GTTTGCTTGA GCTGTGTCGA AACACAAACC GAAAGCAGGC AGCAGCAAAG 
TTCTACAGCT TTTTGGTTCT TAAGAAGCAG CAAGCCATCG AGCTCACACA GGAAGAGCCG 
TACAGTGACA TCATTGCAAC CCCTGGACCA CGGTTCCATA TTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad21 ( XM_006520644.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520707.1 Mus musculus RAD21 cohesin complex component (Rad21), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03314
Clone ID Related Accession (Same CDS sequence)	NM_009009.4 , XM_006520644.1 , XM_006520645.2 , XM_006520645.1
Accession Version	XM_006520644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	double-strand-break repair protein rad21 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTACG CACATTTTGT CCTCAGTAAA CGAGGGCCTC TGGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAGAAGCT AACCAAAGCC CATGTATTTG AGTGCAACTT AGAGAGCAGT 
GTGGAGAGCA TCATCTCACC AAAGGTGAAG ATGGCGCTGC GGACGTCAGG ACACCTTCTC 
CTGGGAGTAG TCCGCATCTA TCACAGGAAA GCCAAATACC TCCTCGCAGA CTGTAATGAA 
GCATTTATTA AAATAAAGAT GGCGTTTCGG CCAGGTGTTG TCGATCTACC TGAGGAAAAT 
CGGGAAGCAG CTTATAATGC CATTACTTTA CCTGAGGAAT TCCACGATTT TGATCAGCCA 
CTGCCAGATT TAGATGATAT TGACGTCGCC CAGCAGTTCA GCCTGAACCA AAGCAGAGTA 
GAAGAGATAA CCATGAGAGA AGAAGTCGGA AACATCAGTA TCCTACAGGA AAATGACTTT 
GGTGACTTCG GAATGGATGA CCGTGAAATA ATGAGAGAAG GCAGTGCTTT CGAGGATGAC 
GACATGTTAG TGAGCACCAG CGCTTCCAAC CTTCTCCTCG AGCCAGAGCA GAGCACCAGC 
AACCTGAATG AAAAGATGAA TCACTTAGAG TACGAAGACC AGTACAAAGA TGACAATTTT 
GGAGAAGGAA ATGATGGCGG TATATTAGAT GACAAACTTA TAAGTAATAA TGATGGTGGC 
ATCTTTGACG ATCCCCCTGC CTTGTCTGAG GCAGGGGTCA TGTTGCCAGA GCAACCTGCA 
CATGATGACA TGGATGAAGA TGACAATGGC TCACTGGGTG GGCCGGATAG TCCCGACTCT 
GTGGATCCTG TCGAACCGAT GCCAACTATG ACTGATCAGA CAACTCTCGT CCCAAACGAG 
GAAGAAGCTT TTGCGTTGGA GCCCATTGAT ATAACTGTCA AAGAGACAAA AGCCAAGAGG 
AAGAGGAAGC TGATTGTTGA CAGTGTCAAA GAATTGGATA GTAAGACCAT TAGAGCCCAG 
CTTAGCGATT ATTCTGATAT TGTTACGACT CTGGACCTGG CTCCGCCAAC CAAGAAGCTT 
ATGATGTGGA AAGAGACAGG AGGAGTGGAA AAGCTCTTCT TTTTACCAGC ACAGCCCCTG 
TGGAATAACC GGCTACTGAA GCTCTTCACA CGCTGCCTTA CCCCACTTGT ACCAGAAGAC 
CTTAGGAAGA GAAGGAAAGG GGGAGAGGCA GATAATCTGG ATGAGTTCCT CAAAGAGTTT 
GAGAATCCAG AGGTTCCCAG AGAGGAGCAG CAGCCACAGC AGCAGCAGCC ACAGCCGCAG 
CGAGATGTCA TCGATGAGCC CATTATAGAA GAGCCAAGCC GCCTCCAGGA CTCAGTGATG 
GAGGCCAGCA GAACAACCAT AGAAGAATCA GCCATGCCCC CACCACCCCC TCAAGGAGTT 
AAGCGGAAAG CCGGGCAAAT AGACCCAGAG CCTTCGATAC CTCCTCAGCA GGTAGAGCAA 
ATGGAAATAC CACCAGTAGA ACTTCCCCCA GAGGAGCCTC CAAATATCTG TCAGCTGATC 
CCGGAGTTAG AGCTCCTACC GGAGAAGGAG AAGGAAAAAG AGAAGGAGAA GGAAGAGGAG 
GAGGAGGAGG AGGATGAAGA TGCTTCAGGG GGTGATCAGG ATCAAGAGGA AAGGAGATGG 
AACAAACGCA CTCAGCAGAT GCTTCATGGT CTTCAGCGAG CTCTTGCTAA AACTGGAGCA 
GAGTCTATCA GTTTGCTTGA GCTGTGTCGA AACACAAACC GAAAGCAGGC AGCAGCAAAG 
TTCTACAGCT TTTTGGTTCT TAAGAAGCAG CAAGCCATCG AGCTCACACA GGAAGAGCCG 
TACAGTGACA TCATTGCAAC CCCTGGACCA CGGTTCCATA TTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520707.1
CDS	161..2068
Translation	MFYAHFVLSKRGPLAKIWLAAHWDKKLTKAHVFECNLESSVESIISPKVKMALRTSGHLLLGVVRIYHRKAKYLLADCNEAFIKIKMAFRPGVVDLPEENREAAYNAITLPEEFHDFDQPLPDLDDIDVAQQFSLNQSRVEEITMREEVGNISILQENDFGDFGMDDREIMREGSAFEDDDMLVSTSASNLLLEPEQSTSNLNEKMNHLEYEDQYKDDNFGEGNDGGILDDKLISNNDGGIFDDPPALSEAGVMLPEQPAHDDMDEDDNGSLGGPDSPDSVDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVTTLDLAPPTKKLMMWKETGGVEKLFFLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEFENPEVPREEQQPQQQQPQPQRDVIDEPIIEEPSRLQDSVMEASRTTIEESAMPPPPPQGVKRKAGQIDPEPSIPPQQVEQMEIPPVELPPEEPPNICQLIPELELLPEKEKEKEKEKEEEEEEEDEDASGGDQDQEERRWNKRTQQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGPRFHII

Target ORF information:

RefSeq Version	XM_006520644.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD21 cohesin complex component (Rad21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520644.1

ORF Insert Sequence:

ATGTTCTACG CACATTTTGT CCTCAGTAAA CGAGGGCCTC TGGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAGAAGCT AACCAAAGCC CATGTATTTG AGTGCAACTT AGAGAGCAGT 
GTGGAGAGCA TCATCTCACC AAAGGTGAAG ATGGCGCTGC GGACGTCAGG ACACCTTCTC 
CTGGGAGTAG TCCGCATCTA TCACAGGAAA GCCAAATACC TCCTCGCAGA CTGTAATGAA 
GCATTTATTA AAATAAAGAT GGCGTTTCGG CCAGGTGTTG TCGATCTACC TGAGGAAAAT 
CGGGAAGCAG CTTATAATGC CATTACTTTA CCTGAGGAAT TCCACGATTT TGATCAGCCA 
CTGCCAGATT TAGATGATAT TGACGTCGCC CAGCAGTTCA GCCTGAACCA AAGCAGAGTA 
GAAGAGATAA CCATGAGAGA AGAAGTCGGA AACATCAGTA TCCTACAGGA AAATGACTTT 
GGTGACTTCG GAATGGATGA CCGTGAAATA ATGAGAGAAG GCAGTGCTTT CGAGGATGAC 
GACATGTTAG TGAGCACCAG CGCTTCCAAC CTTCTCCTCG AGCCAGAGCA GAGCACCAGC 
AACCTGAATG AAAAGATGAA TCACTTAGAG TACGAAGACC AGTACAAAGA TGACAATTTT 
GGAGAAGGAA ATGATGGCGG TATATTAGAT GACAAACTTA TAAGTAATAA TGATGGTGGC 
ATCTTTGACG ATCCCCCTGC CTTGTCTGAG GCAGGGGTCA TGTTGCCAGA GCAACCTGCA 
CATGATGACA TGGATGAAGA TGACAATGGC TCACTGGGTG GGCCGGATAG TCCCGACTCT 
GTGGATCCTG TCGAACCGAT GCCAACTATG ACTGATCAGA CAACTCTCGT CCCAAACGAG 
GAAGAAGCTT TTGCGTTGGA GCCCATTGAT ATAACTGTCA AAGAGACAAA AGCCAAGAGG 
AAGAGGAAGC TGATTGTTGA CAGTGTCAAA GAATTGGATA GTAAGACCAT TAGAGCCCAG 
CTTAGCGATT ATTCTGATAT TGTTACGACT CTGGACCTGG CTCCGCCAAC CAAGAAGCTT 
ATGATGTGGA AAGAGACAGG AGGAGTGGAA AAGCTCTTCT TTTTACCAGC ACAGCCCCTG 
TGGAATAACC GGCTACTGAA GCTCTTCACA CGCTGCCTTA CCCCACTTGT ACCAGAAGAC 
CTTAGGAAGA GAAGGAAAGG GGGAGAGGCA GATAATCTGG ATGAGTTCCT CAAAGAGTTT 
GAGAATCCAG AGGTTCCCAG AGAGGAGCAG CAGCCACAGC AGCAGCAGCC ACAGCCGCAG 
CGAGATGTCA TCGATGAGCC CATTATAGAA GAGCCAAGCC GCCTCCAGGA CTCAGTGATG 
GAGGCCAGCA GAACAACCAT AGAAGAATCA GCCATGCCCC CACCACCCCC TCAAGGAGTT 
AAGCGGAAAG CCGGGCAAAT AGACCCAGAG CCTTCGATAC CTCCTCAGCA GGTAGAGCAA 
ATGGAAATAC CACCAGTAGA ACTTCCCCCA GAGGAGCCTC CAAATATCTG TCAGCTGATC 
CCGGAGTTAG AGCTCCTACC GGAGAAGGAG AAGGAAAAAG AGAAGGAGAA GGAAGAGGAG 
GAGGAGGAGG AGGATGAAGA TGCTTCAGGG GGTGATCAGG ATCAAGAGGA AAGGAGATGG 
AACAAACGCA CTCAGCAGAT GCTTCATGGT CTTCAGCGAG CTCTTGCTAA AACTGGAGCA 
GAGTCTATCA GTTTGCTTGA GCTGTGTCGA AACACAAACC GAAAGCAGGC AGCAGCAAAG 
TTCTACAGCT TTTTGGTTCT TAAGAAGCAG CAAGCCATCG AGCTCACACA GGAAGAGCCG 
TACAGTGACA TCATTGCAAC CCCTGGACCA CGGTTCCATA TTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad21 ( XM_006520645.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520708.1 Mus musculus RAD21 cohesin complex component (Rad21), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu03314
Clone ID Related Accession (Same CDS sequence)	NM_009009.4 , XM_006520644.1 , XM_006520645.2 , XM_006520645.1
Accession Version	XM_006520645.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	double-strand-break repair protein rad21 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTACG CACATTTTGT CCTCAGTAAA CGAGGGCCTC TGGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAGAAGCT AACCAAAGCC CATGTATTTG AGTGCAACTT AGAGAGCAGT 
GTGGAGAGCA TCATCTCACC AAAGGTGAAG ATGGCGCTGC GGACGTCAGG ACACCTTCTC 
CTGGGAGTAG TCCGCATCTA TCACAGGAAA GCCAAATACC TCCTCGCAGA CTGTAATGAA 
GCATTTATTA AAATAAAGAT GGCGTTTCGG CCAGGTGTTG TCGATCTACC TGAGGAAAAT 
CGGGAAGCAG CTTATAATGC CATTACTTTA CCTGAGGAAT TCCACGATTT TGATCAGCCA 
CTGCCAGATT TAGATGATAT TGACGTCGCC CAGCAGTTCA GCCTGAACCA AAGCAGAGTA 
GAAGAGATAA CCATGAGAGA AGAAGTCGGA AACATCAGTA TCCTACAGGA AAATGACTTT 
GGTGACTTCG GAATGGATGA CCGTGAAATA ATGAGAGAAG GCAGTGCTTT CGAGGATGAC 
GACATGTTAG TGAGCACCAG CGCTTCCAAC CTTCTCCTCG AGCCAGAGCA GAGCACCAGC 
AACCTGAATG AAAAGATGAA TCACTTAGAG TACGAAGACC AGTACAAAGA TGACAATTTT 
GGAGAAGGAA ATGATGGCGG TATATTAGAT GACAAACTTA TAAGTAATAA TGATGGTGGC 
ATCTTTGACG ATCCCCCTGC CTTGTCTGAG GCAGGGGTCA TGTTGCCAGA GCAACCTGCA 
CATGATGACA TGGATGAAGA TGACAATGGC TCACTGGGTG GGCCGGATAG TCCCGACTCT 
GTGGATCCTG TCGAACCGAT GCCAACTATG ACTGATCAGA CAACTCTCGT CCCAAACGAG 
GAAGAAGCTT TTGCGTTGGA GCCCATTGAT ATAACTGTCA AAGAGACAAA AGCCAAGAGG 
AAGAGGAAGC TGATTGTTGA CAGTGTCAAA GAATTGGATA GTAAGACCAT TAGAGCCCAG 
CTTAGCGATT ATTCTGATAT TGTTACGACT CTGGACCTGG CTCCGCCAAC CAAGAAGCTT 
ATGATGTGGA AAGAGACAGG AGGAGTGGAA AAGCTCTTCT TTTTACCAGC ACAGCCCCTG 
TGGAATAACC GGCTACTGAA GCTCTTCACA CGCTGCCTTA CCCCACTTGT ACCAGAAGAC 
CTTAGGAAGA GAAGGAAAGG GGGAGAGGCA GATAATCTGG ATGAGTTCCT CAAAGAGTTT 
GAGAATCCAG AGGTTCCCAG AGAGGAGCAG CAGCCACAGC AGCAGCAGCC ACAGCCGCAG 
CGAGATGTCA TCGATGAGCC CATTATAGAA GAGCCAAGCC GCCTCCAGGA CTCAGTGATG 
GAGGCCAGCA GAACAACCAT AGAAGAATCA GCCATGCCCC CACCACCCCC TCAAGGAGTT 
AAGCGGAAAG CCGGGCAAAT AGACCCAGAG CCTTCGATAC CTCCTCAGCA GGTAGAGCAA 
ATGGAAATAC CACCAGTAGA ACTTCCCCCA GAGGAGCCTC CAAATATCTG TCAGCTGATC 
CCGGAGTTAG AGCTCCTACC GGAGAAGGAG AAGGAAAAAG AGAAGGAGAA GGAAGAGGAG 
GAGGAGGAGG AGGATGAAGA TGCTTCAGGG GGTGATCAGG ATCAAGAGGA AAGGAGATGG 
AACAAACGCA CTCAGCAGAT GCTTCATGGT CTTCAGCGAG CTCTTGCTAA AACTGGAGCA 
GAGTCTATCA GTTTGCTTGA GCTGTGTCGA AACACAAACC GAAAGCAGGC AGCAGCAAAG 
TTCTACAGCT TTTTGGTTCT TAAGAAGCAG CAAGCCATCG AGCTCACACA GGAAGAGCCG 
TACAGTGACA TCATTGCAAC CCCTGGACCA CGGTTCCATA TTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520708.1
CDS	62..1969
Translation	MFYAHFVLSKRGPLAKIWLAAHWDKKLTKAHVFECNLESSVESIISPKVKMALRTSGHLLLGVVRIYHRKAKYLLADCNEAFIKIKMAFRPGVVDLPEENREAAYNAITLPEEFHDFDQPLPDLDDIDVAQQFSLNQSRVEEITMREEVGNISILQENDFGDFGMDDREIMREGSAFEDDDMLVSTSASNLLLEPEQSTSNLNEKMNHLEYEDQYKDDNFGEGNDGGILDDKLISNNDGGIFDDPPALSEAGVMLPEQPAHDDMDEDDNGSLGGPDSPDSVDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVTTLDLAPPTKKLMMWKETGGVEKLFFLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEFENPEVPREEQQPQQQQPQPQRDVIDEPIIEEPSRLQDSVMEASRTTIEESAMPPPPPQGVKRKAGQIDPEPSIPPQQVEQMEIPPVELPPEEPPNICQLIPELELLPEKEKEKEKEKEEEEEEEDEDASGGDQDQEERRWNKRTQQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGPRFHII

Target ORF information:

RefSeq Version	XM_006520645.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD21 cohesin complex component (Rad21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520645.1

ORF Insert Sequence:

ATGTTCTACG CACATTTTGT CCTCAGTAAA CGAGGGCCTC TGGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAGAAGCT AACCAAAGCC CATGTATTTG AGTGCAACTT AGAGAGCAGT 
GTGGAGAGCA TCATCTCACC AAAGGTGAAG ATGGCGCTGC GGACGTCAGG ACACCTTCTC 
CTGGGAGTAG TCCGCATCTA TCACAGGAAA GCCAAATACC TCCTCGCAGA CTGTAATGAA 
GCATTTATTA AAATAAAGAT GGCGTTTCGG CCAGGTGTTG TCGATCTACC TGAGGAAAAT 
CGGGAAGCAG CTTATAATGC CATTACTTTA CCTGAGGAAT TCCACGATTT TGATCAGCCA 
CTGCCAGATT TAGATGATAT TGACGTCGCC CAGCAGTTCA GCCTGAACCA AAGCAGAGTA 
GAAGAGATAA CCATGAGAGA AGAAGTCGGA AACATCAGTA TCCTACAGGA AAATGACTTT 
GGTGACTTCG GAATGGATGA CCGTGAAATA ATGAGAGAAG GCAGTGCTTT CGAGGATGAC 
GACATGTTAG TGAGCACCAG CGCTTCCAAC CTTCTCCTCG AGCCAGAGCA GAGCACCAGC 
AACCTGAATG AAAAGATGAA TCACTTAGAG TACGAAGACC AGTACAAAGA TGACAATTTT 
GGAGAAGGAA ATGATGGCGG TATATTAGAT GACAAACTTA TAAGTAATAA TGATGGTGGC 
ATCTTTGACG ATCCCCCTGC CTTGTCTGAG GCAGGGGTCA TGTTGCCAGA GCAACCTGCA 
CATGATGACA TGGATGAAGA TGACAATGGC TCACTGGGTG GGCCGGATAG TCCCGACTCT 
GTGGATCCTG TCGAACCGAT GCCAACTATG ACTGATCAGA CAACTCTCGT CCCAAACGAG 
GAAGAAGCTT TTGCGTTGGA GCCCATTGAT ATAACTGTCA AAGAGACAAA AGCCAAGAGG 
AAGAGGAAGC TGATTGTTGA CAGTGTCAAA GAATTGGATA GTAAGACCAT TAGAGCCCAG 
CTTAGCGATT ATTCTGATAT TGTTACGACT CTGGACCTGG CTCCGCCAAC CAAGAAGCTT 
ATGATGTGGA AAGAGACAGG AGGAGTGGAA AAGCTCTTCT TTTTACCAGC ACAGCCCCTG 
TGGAATAACC GGCTACTGAA GCTCTTCACA CGCTGCCTTA CCCCACTTGT ACCAGAAGAC 
CTTAGGAAGA GAAGGAAAGG GGGAGAGGCA GATAATCTGG ATGAGTTCCT CAAAGAGTTT 
GAGAATCCAG AGGTTCCCAG AGAGGAGCAG CAGCCACAGC AGCAGCAGCC ACAGCCGCAG 
CGAGATGTCA TCGATGAGCC CATTATAGAA GAGCCAAGCC GCCTCCAGGA CTCAGTGATG 
GAGGCCAGCA GAACAACCAT AGAAGAATCA GCCATGCCCC CACCACCCCC TCAAGGAGTT 
AAGCGGAAAG CCGGGCAAAT AGACCCAGAG CCTTCGATAC CTCCTCAGCA GGTAGAGCAA 
ATGGAAATAC CACCAGTAGA ACTTCCCCCA GAGGAGCCTC CAAATATCTG TCAGCTGATC 
CCGGAGTTAG AGCTCCTACC GGAGAAGGAG AAGGAAAAAG AGAAGGAGAA GGAAGAGGAG 
GAGGAGGAGG AGGATGAAGA TGCTTCAGGG GGTGATCAGG ATCAAGAGGA AAGGAGATGG 
AACAAACGCA CTCAGCAGAT GCTTCATGGT CTTCAGCGAG CTCTTGCTAA AACTGGAGCA 
GAGTCTATCA GTTTGCTTGA GCTGTGTCGA AACACAAACC GAAAGCAGGC AGCAGCAAAG 
TTCTACAGCT TTTTGGTTCT TAAGAAGCAG CAAGCCATCG AGCTCACACA GGAAGAGCCG 
TACAGTGACA TCATTGCAAC CCCTGGACCA CGGTTCCATA TTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad21 ( XM_006520645.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520708.1 Mus musculus RAD21 cohesin complex component (Rad21), transcript variant X2, mRNA.

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CloneID	OMu03314
Clone ID Related Accession (Same CDS sequence)	NM_009009.4 , XM_006520644.1 , XM_006520645.2 , XM_006520645.1
Accession Version	XM_006520645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	double-strand-break repair protein rad21 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTACG CACATTTTGT CCTCAGTAAA CGAGGGCCTC TGGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAGAAGCT AACCAAAGCC CATGTATTTG AGTGCAACTT AGAGAGCAGT 
GTGGAGAGCA TCATCTCACC AAAGGTGAAG ATGGCGCTGC GGACGTCAGG ACACCTTCTC 
CTGGGAGTAG TCCGCATCTA TCACAGGAAA GCCAAATACC TCCTCGCAGA CTGTAATGAA 
GCATTTATTA AAATAAAGAT GGCGTTTCGG CCAGGTGTTG TCGATCTACC TGAGGAAAAT 
CGGGAAGCAG CTTATAATGC CATTACTTTA CCTGAGGAAT TCCACGATTT TGATCAGCCA 
CTGCCAGATT TAGATGATAT TGACGTCGCC CAGCAGTTCA GCCTGAACCA AAGCAGAGTA 
GAAGAGATAA CCATGAGAGA AGAAGTCGGA AACATCAGTA TCCTACAGGA AAATGACTTT 
GGTGACTTCG GAATGGATGA CCGTGAAATA ATGAGAGAAG GCAGTGCTTT CGAGGATGAC 
GACATGTTAG TGAGCACCAG CGCTTCCAAC CTTCTCCTCG AGCCAGAGCA GAGCACCAGC 
AACCTGAATG AAAAGATGAA TCACTTAGAG TACGAAGACC AGTACAAAGA TGACAATTTT 
GGAGAAGGAA ATGATGGCGG TATATTAGAT GACAAACTTA TAAGTAATAA TGATGGTGGC 
ATCTTTGACG ATCCCCCTGC CTTGTCTGAG GCAGGGGTCA TGTTGCCAGA GCAACCTGCA 
CATGATGACA TGGATGAAGA TGACAATGGC TCACTGGGTG GGCCGGATAG TCCCGACTCT 
GTGGATCCTG TCGAACCGAT GCCAACTATG ACTGATCAGA CAACTCTCGT CCCAAACGAG 
GAAGAAGCTT TTGCGTTGGA GCCCATTGAT ATAACTGTCA AAGAGACAAA AGCCAAGAGG 
AAGAGGAAGC TGATTGTTGA CAGTGTCAAA GAATTGGATA GTAAGACCAT TAGAGCCCAG 
CTTAGCGATT ATTCTGATAT TGTTACGACT CTGGACCTGG CTCCGCCAAC CAAGAAGCTT 
ATGATGTGGA AAGAGACAGG AGGAGTGGAA AAGCTCTTCT TTTTACCAGC ACAGCCCCTG 
TGGAATAACC GGCTACTGAA GCTCTTCACA CGCTGCCTTA CCCCACTTGT ACCAGAAGAC 
CTTAGGAAGA GAAGGAAAGG GGGAGAGGCA GATAATCTGG ATGAGTTCCT CAAAGAGTTT 
GAGAATCCAG AGGTTCCCAG AGAGGAGCAG CAGCCACAGC AGCAGCAGCC ACAGCCGCAG 
CGAGATGTCA TCGATGAGCC CATTATAGAA GAGCCAAGCC GCCTCCAGGA CTCAGTGATG 
GAGGCCAGCA GAACAACCAT AGAAGAATCA GCCATGCCCC CACCACCCCC TCAAGGAGTT 
AAGCGGAAAG CCGGGCAAAT AGACCCAGAG CCTTCGATAC CTCCTCAGCA GGTAGAGCAA 
ATGGAAATAC CACCAGTAGA ACTTCCCCCA GAGGAGCCTC CAAATATCTG TCAGCTGATC 
CCGGAGTTAG AGCTCCTACC GGAGAAGGAG AAGGAAAAAG AGAAGGAGAA GGAAGAGGAG 
GAGGAGGAGG AGGATGAAGA TGCTTCAGGG GGTGATCAGG ATCAAGAGGA AAGGAGATGG 
AACAAACGCA CTCAGCAGAT GCTTCATGGT CTTCAGCGAG CTCTTGCTAA AACTGGAGCA 
GAGTCTATCA GTTTGCTTGA GCTGTGTCGA AACACAAACC GAAAGCAGGC AGCAGCAAAG 
TTCTACAGCT TTTTGGTTCT TAAGAAGCAG CAAGCCATCG AGCTCACACA GGAAGAGCCG 
TACAGTGACA TCATTGCAAC CCCTGGACCA CGGTTCCATA TTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520708.1
CDS	351..2258
Translation	MFYAHFVLSKRGPLAKIWLAAHWDKKLTKAHVFECNLESSVESIISPKVKMALRTSGHLLLGVVRIYHRKAKYLLADCNEAFIKIKMAFRPGVVDLPEENREAAYNAITLPEEFHDFDQPLPDLDDIDVAQQFSLNQSRVEEITMREEVGNISILQENDFGDFGMDDREIMREGSAFEDDDMLVSTSASNLLLEPEQSTSNLNEKMNHLEYEDQYKDDNFGEGNDGGILDDKLISNNDGGIFDDPPALSEAGVMLPEQPAHDDMDEDDNGSLGGPDSPDSVDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVTTLDLAPPTKKLMMWKETGGVEKLFFLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEFENPEVPREEQQPQQQQPQPQRDVIDEPIIEEPSRLQDSVMEASRTTIEESAMPPPPPQGVKRKAGQIDPEPSIPPQQVEQMEIPPVELPPEEPPNICQLIPELELLPEKEKEKEKEKEEEEEEEDEDASGGDQDQEERRWNKRTQQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGPRFHII

Target ORF information:

RefSeq Version	XM_006520645.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD21 cohesin complex component (Rad21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520645.2

ORF Insert Sequence:

ATGTTCTACG CACATTTTGT CCTCAGTAAA CGAGGGCCTC TGGCCAAAAT CTGGCTGGCG 
GCCCATTGGG ACAAGAAGCT AACCAAAGCC CATGTATTTG AGTGCAACTT AGAGAGCAGT 
GTGGAGAGCA TCATCTCACC AAAGGTGAAG ATGGCGCTGC GGACGTCAGG ACACCTTCTC 
CTGGGAGTAG TCCGCATCTA TCACAGGAAA GCCAAATACC TCCTCGCAGA CTGTAATGAA 
GCATTTATTA AAATAAAGAT GGCGTTTCGG CCAGGTGTTG TCGATCTACC TGAGGAAAAT 
CGGGAAGCAG CTTATAATGC CATTACTTTA CCTGAGGAAT TCCACGATTT TGATCAGCCA 
CTGCCAGATT TAGATGATAT TGACGTCGCC CAGCAGTTCA GCCTGAACCA AAGCAGAGTA 
GAAGAGATAA CCATGAGAGA AGAAGTCGGA AACATCAGTA TCCTACAGGA AAATGACTTT 
GGTGACTTCG GAATGGATGA CCGTGAAATA ATGAGAGAAG GCAGTGCTTT CGAGGATGAC 
GACATGTTAG TGAGCACCAG CGCTTCCAAC CTTCTCCTCG AGCCAGAGCA GAGCACCAGC 
AACCTGAATG AAAAGATGAA TCACTTAGAG TACGAAGACC AGTACAAAGA TGACAATTTT 
GGAGAAGGAA ATGATGGCGG TATATTAGAT GACAAACTTA TAAGTAATAA TGATGGTGGC 
ATCTTTGACG ATCCCCCTGC CTTGTCTGAG GCAGGGGTCA TGTTGCCAGA GCAACCTGCA 
CATGATGACA TGGATGAAGA TGACAATGGC TCACTGGGTG GGCCGGATAG TCCCGACTCT 
GTGGATCCTG TCGAACCGAT GCCAACTATG ACTGATCAGA CAACTCTCGT CCCAAACGAG 
GAAGAAGCTT TTGCGTTGGA GCCCATTGAT ATAACTGTCA AAGAGACAAA AGCCAAGAGG 
AAGAGGAAGC TGATTGTTGA CAGTGTCAAA GAATTGGATA GTAAGACCAT TAGAGCCCAG 
CTTAGCGATT ATTCTGATAT TGTTACGACT CTGGACCTGG CTCCGCCAAC CAAGAAGCTT 
ATGATGTGGA AAGAGACAGG AGGAGTGGAA AAGCTCTTCT TTTTACCAGC ACAGCCCCTG 
TGGAATAACC GGCTACTGAA GCTCTTCACA CGCTGCCTTA CCCCACTTGT ACCAGAAGAC 
CTTAGGAAGA GAAGGAAAGG GGGAGAGGCA GATAATCTGG ATGAGTTCCT CAAAGAGTTT 
GAGAATCCAG AGGTTCCCAG AGAGGAGCAG CAGCCACAGC AGCAGCAGCC ACAGCCGCAG 
CGAGATGTCA TCGATGAGCC CATTATAGAA GAGCCAAGCC GCCTCCAGGA CTCAGTGATG 
GAGGCCAGCA GAACAACCAT AGAAGAATCA GCCATGCCCC CACCACCCCC TCAAGGAGTT 
AAGCGGAAAG CCGGGCAAAT AGACCCAGAG CCTTCGATAC CTCCTCAGCA GGTAGAGCAA 
ATGGAAATAC CACCAGTAGA ACTTCCCCCA GAGGAGCCTC CAAATATCTG TCAGCTGATC 
CCGGAGTTAG AGCTCCTACC GGAGAAGGAG AAGGAAAAAG AGAAGGAGAA GGAAGAGGAG 
GAGGAGGAGG AGGATGAAGA TGCTTCAGGG GGTGATCAGG ATCAAGAGGA AAGGAGATGG 
AACAAACGCA CTCAGCAGAT GCTTCATGGT CTTCAGCGAG CTCTTGCTAA AACTGGAGCA 
GAGTCTATCA GTTTGCTTGA GCTGTGTCGA AACACAAACC GAAAGCAGGC AGCAGCAAAG 
TTCTACAGCT TTTTGGTTCT TAAGAAGCAG CAAGCCATCG AGCTCACACA GGAAGAGCCG 
TACAGTGACA TCATTGCAAC CCCTGGACCA CGGTTCCATA TTATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A mechanism of cohesin-dependent loop extrusion organizes zygotic genome architecture.
The EMBO journal36(24)3600-3618(2017 12)
Gassler J,Brand?o HB,Imakaev M,Flyamer IM,Ladst?tter S,Bickmore WA,Peters JM,Mirny LA,Tachibana K

5C analysis of the Epidermal Differentiation Complex locus reveals distinct chromatin interaction networks between gene-rich and gene-poor TADs in skin epithelial cells.
PLoS genetics13(9)e1006966(2017 Sep)
Poterlowicz K,Yarker JL,Malashchuk I,Lajoie BR,Mardaryev AN,Gdula MR,Sharov AA,Kohwi-Shigematsu T,Botchkarev VA,Fessing MY

The cohesin subunit Rad21 is a negative regulator of hematopoietic self-renewal through epigenetic repression of Hoxa7 and Hoxa9.
Leukemia31(3)712-719(2017 Mar)
Fisher JB,Peterson J,Reimer M,Stelloh C,Pulakanti K,Gerbec ZJ,Abel AM,Strouse JM,Strouse C,McNulty M,Malarkannan S,Crispino JD,Milanovich S,Rao S

A Surveillance Mechanism Ensures Repair of DNA Lesions during Zygotic Reprogramming.
Cell167(7)1774-1787.e13(2016 Dec)
Ladst?tter S,Tachibana-Konwalski K

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Rad21 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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