Kif20a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kif20a
Entrez Gene ID	19348
Full Name	kinesin family member 20A
Synonyms	AA415432,Rab6kifl
General protein information	Preferred Names kinesin family member 20A Names kinesin-like protein KIF20A Rabkinesin-6 kinesin-like protein 174 rab6-interacting kinesin-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 B1\|18 18.69 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001166407.1	NP_001159879.1	kinesin-like protein KIF20A
NM_009004.4	NP_033030.1	kinesin-like protein KIF20A
NM_001166406.1	NP_001159878.1	kinesin-like protein KIF20A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kif20a	NP_001101896.1
	KIF20A	XP_001107307.1
Bos taurus (cattle)	KIF20A	NP_001039753.1
Pan troglodytes (chimpanzee)	KIF20A	XP_001170694.1
Gallus gallus (chicken)	KIF20A	NP_001012801.1
Homo sapiens (human)	KIF20A	NP_005724.1
Mus musculus (house mouse)	Kif20a	NP_001159878.1
Xenopus tropicalis (tropical clawed frog)	kif20a	XP_002936881.1
Danio rerio (zebrafish)	LOC325449	NP_001154942.1

Gene Ontology
Bibliography

Related articles in PubMed

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Naiman N, Fujioka K, Fujino M, Valerius MT, Potter SS, McMahon AP, Kobayashi A
Developmental cell41(4)349-365.e3(2017 05)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

The vertebrate-specific Kinesin-6, Kif20b, is required for normal cytokinesis of polarized cortical stem cells and cerebral cortex size.
Janisch KM, Vock VM, Fleming MS, Shrestha A, Grimsley-Myers CM, Rasoul BA, Neale SA, Cupp TD, Kinchen JM, Liem KF Jr, Dwyer ND
Development (Cambridge, England)140(23)4672-82(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Kif20a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kif20a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19128	NM_001166407.1 Latest version!	Mus musculus kinesin family member 20A (Kif20a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu19128	NM_009004.4 Latest version!	Mus musculus kinesin family member 20A (Kif20a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu19128	NM_001166406.1 Latest version!	Mus musculus kinesin family member 20A (Kif20a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kif20a ( NM_001166407.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001159879.1 Mus musculus kinesin family member 20A (Kif20a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19128
Clone ID Related Accession (Same CDS sequence)	NM_001166407.1 , NM_009004.4 , NM_001166406.1
Accession Version	NM_001166407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF20A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074335.11, AK144844.1 and AK084732.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon, as compared to variant 1. Variants 1-3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK084732.1, Y09632.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCACC GGATCCTTTC TCCGCCAGCG GGCTTACTCT CTGATGAGGA TGTTGTAGAC 
TCTCCCATAT TAGAATCCAC AGCTGCAGAT CTGAGATCTG TTGTCCGAAA GGACCTGTTG 
TCAGACTGCT CTGTCATCTC TGCCTCTCTG GAGGACAAGC AGGCTCTACT GGAGGACACC 
TCAGAGAAGG TGAAAGTTTA CCTTCGAATT CGACCATTCT TGACTTCAGA GTTGGACCGA 
CAGGAAGATC AGGGCTGCGT CTGCATTGAG AATACAGAAA CCCTTGTGCT GCAGGCACCC 
AAAGACTCCT TTGCCTTGAA GAGTAATGAG AGGGGAGTTG GTCAGGCCAC TCACAAATTT 
ACCTTTTCCC AGATATTTGG GCCAGAAGTG GGACAGGTGG CCTTCTTCAA TCTGACCATG 
AAGGAGATGG TGAAGGATGT GCTCAAAGGG CAGAACTGGC TCATCTACAC CTATGGAGTC 
ACCAACTCGG GGAAAACGTA CACCATTCAA GGTACTAGTA AGGATGCGGG GATCCTGCCC 
CAGTCCCTGG CTCTCATCTT TAATAGTCTC CAGGGCCAGC TTCATCCGAC ACCTGACCTG 
AAGCCCTTAT TGTCCAATGA AGTAATCTGG CTAGACAGCA AGCAGATTCG ACAGGAGGAA 
ATGAAGAAGC TGTCCTTGCT AATTGGAGGC CTCCAAGAGG AGGAGCTGTC CACCTCCGTG 
AAGAAACGTG TCCACACTGA AAGTCGGATA GGTGCCAGCA ACAGCTTTGA CAGTGGCGTT 
GCTGGGCTGT CTTCCACCAG TCAGTTCACC AGCAGCAGCC AGCTAGATGA AACAAGTCAA 
TTATGGGCAC AACCAGACAC TGTCCCAGTA AGTGTTCCAG CAGACATTCG CTTCTCTGTC 
TGGATCTCCT TCTTTGAGAT CTACAATGAA TTGCTTTATG ACCTGTTAGA ACCACCTAGC 
CATCAGCATA AGAGACAGAC ACTTCGGCTG TGTGAGGATC AGAACGGCAA TCCTTACGTG 
AAAGATCTCA ATTGGATTCA TGTTCGTGAT GTTGAGGAAG CCTGGAAACT ATTGAAAGTG 
GGTCGCAAGA ACCAGAGCTT TGCCAGCACC CACATGAACC AGCAATCCAG CCGCAGTCAC 
AGCATCTTCT CAATCAGAAT CTTGCACCTT CAGGGAGAAG GGGATATAGT GCCCAAGATC 
AGTGAACTGT CACTCTGTGA CCTGGCTGGC TCTGAGCGCT GCAAACATCA AAAAAGTGGT 
GAGCGGCTAA AGGAGGCAGG GAACATTAAC ACTTCTCTGC ACACCCTGGG CCGCTGTATT 
GCTGCCCTGC GACAGAATCA GCAGAACCGG TCAAAGCAGA ACCTGATTCC TTTCCGTGAC 
AGCAAGTTGA CTCGTGTGTT CCAAGGCTTC TTCACAGGTC GAGGTCGTTC CTGTATGATT 
GTCAATGTGA ATCCCTGTGC ATCTACATAT GACGAGACTC TTCATGCAGC CAAATTCTCA 
GCCCTAGCCA GCCAGCTTGT GCACGCCCCA CCTGTGCATC TGGGAATCCC ATCCCTGCAT 
TCATTCATCA AGAAGCACAG TCCTCAGGTT GGCCCTGGCT TAGAGAAAGA GGACAAGGCC 
GACTCAGACC TTGAGGACAG TCCTGAAGAC GAAGCTGACG TCTCCGTGTA TGGCAAGGAG 
GAACTACTCC AGGTGGTGGA AGCCATGAAA GCGCTACTTT TAAAAGAACG ACAAGAAAAG 
CTGCAGTTGG AGATACAGCT CCGAGAGGAA ATTTGCAACG AAATGGTGGA ACAGATGCAA 
CAGCGGGAGC AGTGGTGCAG TGAGCGTTTG GACAACCAAA AGGAACTGAT GGAGGAACTG 
TACGAGGAGA AACTGAAGAT CCTGAAGGAA TCACTGACAA CTTTCTACCA AGAGCAGATC 
CAGGAGCGGG ATGAAAAAAT TGAAGAGCTA GAAACTCTGT TGCAGGAAGC CAAACAGCAG 
CCAGCGGCAC AACAGTCAGG GGGGCTGTCC CTCCTCCGGC GGTCACAGAG GCTGGCAGCG 
TCTGCCTCCA CTCAGCAGTT CCAGGAAGTT AAAGCTGAAC TAGAGCAATG CAAAACAGAG 
TTAAGCTCTA CCACTGCAGA GCTGCACAAG TATCAGCAAG TGTTAAAACC GCCACCCCCA 
GCCAAGCCTT TCACCATTGA TGTGGATAAG AAGTTAGAGG AGGGCCAGAA GAATATAAGA 
CTGCTACGGA CAGAGCTCCA GAAACTAGGA CAGTCTCTGC AGTCAGCAGA AAGAGCCTGT 
TGCCACAGCA CCGGAGCAGG AAAACTTCGT CAAGCATTAA CCAACTGTGA TGACATCTTA 
ATCAAGCAGA ACCAGACCCT GGCTGAGCTG CAGAATAACA TGGTGCTAGT GAAACTGGAC 
CTTCAGAAAA AGGCAGCCTG CATTGCTGAG CAGTATCACA CTGTGCTAAA GCTCCAAGGC 
CAAGCCTCTG CCAAAAAGCG GCTGGGAGCA AACCAGGAAA ACCAGCAACC AAATCATCAA 
CCCCCAGGAA AGAAACCATT CCTGCGAAAC TTACTTCCCC GAACACCTAC CTGCCAAAGC 
TCAACAGACA GCAGCCCTTA TGCGCGGATC TTGCGCTCAC GGCACTCTCC TTTACTCAAA 
TCTCCCTTTG GCAAAAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159879.1
CDS	252..2915
Translation	MSHRILSPPAGLLSDEDVVDSPILESTAADLRSVVRKDLLSDCSVISASLEDKQALLEDTSEKVKVYLRIRPFLTSELDRQEDQGCVCIENTETLVLQAPKDSFALKSNERGVGQATHKFTFSQIFGPEVGQVAFFNLTMKEMVKDVLKGQNWLIYTYGVTNSGKTYTIQGTSKDAGILPQSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIRQEEMKKLSLLIGGLQEEELSTSVKKRVHTESRIGASNSFDSGVAGLSSTSQFTSSSQLDETSQLWAQPDTVPVSVPADIRFSVWISFFEIYNELLYDLLEPPSHQHKRQTLRLCEDQNGNPYVKDLNWIHVRDVEEAWKLLKVGRKNQSFASTHMNQQSSRSHSIFSIRILHLQGEGDIVPKISELSLCDLAGSERCKHQKSGERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLIPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHAAKFSALASQLVHAPPVHLGIPSLHSFIKKHSPQVGPGLEKEDKADSDLEDSPEDEADVSVYGKEELLQVVEAMKALLLKERQEKLQLEIQLREEICNEMVEQMQQREQWCSERLDNQKELMEELYEEKLKILKESLTTFYQEQIQERDEKIEELETLLQEAKQQPAAQQSGGLSLLRRSQRLAASASTQQFQEVKAELEQCKTELSSTTAELHKYQQVLKPPPPAKPFTIDVDKKLEEGQKNIRLLRTELQKLGQSLQSAERACCHSTGAGKLRQALTNCDDILIKQNQTLAELQNNMVLVKLDLQKKAACIAEQYHTVLKLQGQASAKKRLGANQENQQPNHQPPGKKPFLRNLLPRTPTCQSSTDSSPYARILRSRHSPLLKSPFGKKY

Target ORF information:

RefSeq Version	NM_001166407.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 20A (Kif20a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166407.1

ORF Insert Sequence:

ATGTCTCACC GGATCCTTTC TCCGCCAGCG GGCTTACTCT CTGATGAGGA TGTTGTAGAC 
TCTCCCATAT TAGAATCCAC AGCTGCAGAT CTGAGATCTG TTGTCCGAAA GGACCTGTTG 
TCAGACTGCT CTGTCATCTC TGCCTCTCTG GAGGACAAGC AGGCTCTACT GGAGGACACC 
TCAGAGAAGG TGAAAGTTTA CCTTCGAATT CGACCATTCT TGACTTCAGA GTTGGACCGA 
CAGGAAGATC AGGGCTGCGT CTGCATTGAG AATACAGAAA CCCTTGTGCT GCAGGCACCC 
AAAGACTCCT TTGCCTTGAA GAGTAATGAG AGGGGAGTTG GTCAGGCCAC TCACAAATTT 
ACCTTTTCCC AGATATTTGG GCCAGAAGTG GGACAGGTGG CCTTCTTCAA TCTGACCATG 
AAGGAGATGG TGAAGGATGT GCTCAAAGGG CAGAACTGGC TCATCTACAC CTATGGAGTC 
ACCAACTCGG GGAAAACGTA CACCATTCAA GGTACTAGTA AGGATGCGGG GATCCTGCCC 
CAGTCCCTGG CTCTCATCTT TAATAGTCTC CAGGGCCAGC TTCATCCGAC ACCTGACCTG 
AAGCCCTTAT TGTCCAATGA AGTAATCTGG CTAGACAGCA AGCAGATTCG ACAGGAGGAA 
ATGAAGAAGC TGTCCTTGCT AATTGGAGGC CTCCAAGAGG AGGAGCTGTC CACCTCCGTG 
AAGAAACGTG TCCACACTGA AAGTCGGATA GGTGCCAGCA ACAGCTTTGA CAGTGGCGTT 
GCTGGGCTGT CTTCCACCAG TCAGTTCACC AGCAGCAGCC AGCTAGATGA AACAAGTCAA 
TTATGGGCAC AACCAGACAC TGTCCCAGTA AGTGTTCCAG CAGACATTCG CTTCTCTGTC 
TGGATCTCCT TCTTTGAGAT CTACAATGAA TTGCTTTATG ACCTGTTAGA ACCACCTAGC 
CATCAGCATA AGAGACAGAC ACTTCGGCTG TGTGAGGATC AGAACGGCAA TCCTTACGTG 
AAAGATCTCA ATTGGATTCA TGTTCGTGAT GTTGAGGAAG CCTGGAAACT ATTGAAAGTG 
GGTCGCAAGA ACCAGAGCTT TGCCAGCACC CACATGAACC AGCAATCCAG CCGCAGTCAC 
AGCATCTTCT CAATCAGAAT CTTGCACCTT CAGGGAGAAG GGGATATAGT GCCCAAGATC 
AGTGAACTGT CACTCTGTGA CCTGGCTGGC TCTGAGCGCT GCAAACATCA AAAAAGTGGT 
GAGCGGCTAA AGGAGGCAGG GAACATTAAC ACTTCTCTGC ACACCCTGGG CCGCTGTATT 
GCTGCCCTGC GACAGAATCA GCAGAACCGG TCAAAGCAGA ACCTGATTCC TTTCCGTGAC 
AGCAAGTTGA CTCGTGTGTT CCAAGGCTTC TTCACAGGTC GAGGTCGTTC CTGTATGATT 
GTCAATGTGA ATCCCTGTGC ATCTACATAT GACGAGACTC TTCATGCAGC CAAATTCTCA 
GCCCTAGCCA GCCAGCTTGT GCACGCCCCA CCTGTGCATC TGGGAATCCC ATCCCTGCAT 
TCATTCATCA AGAAGCACAG TCCTCAGGTT GGCCCTGGCT TAGAGAAAGA GGACAAGGCC 
GACTCAGACC TTGAGGACAG TCCTGAAGAC GAAGCTGACG TCTCCGTGTA TGGCAAGGAG 
GAACTACTCC AGGTGGTGGA AGCCATGAAA GCGCTACTTT TAAAAGAACG ACAAGAAAAG 
CTGCAGTTGG AGATACAGCT CCGAGAGGAA ATTTGCAACG AAATGGTGGA ACAGATGCAA 
CAGCGGGAGC AGTGGTGCAG TGAGCGTTTG GACAACCAAA AGGAACTGAT GGAGGAACTG 
TACGAGGAGA AACTGAAGAT CCTGAAGGAA TCACTGACAA CTTTCTACCA AGAGCAGATC 
CAGGAGCGGG ATGAAAAAAT TGAAGAGCTA GAAACTCTGT TGCAGGAAGC CAAACAGCAG 
CCAGCGGCAC AACAGTCAGG GGGGCTGTCC CTCCTCCGGC GGTCACAGAG GCTGGCAGCG 
TCTGCCTCCA CTCAGCAGTT CCAGGAAGTT AAAGCTGAAC TAGAGCAATG CAAAACAGAG 
TTAAGCTCTA CCACTGCAGA GCTGCACAAG TATCAGCAAG TGTTAAAACC GCCACCCCCA 
GCCAAGCCTT TCACCATTGA TGTGGATAAG AAGTTAGAGG AGGGCCAGAA GAATATAAGA 
CTGCTACGGA CAGAGCTCCA GAAACTAGGA CAGTCTCTGC AGTCAGCAGA AAGAGCCTGT 
TGCCACAGCA CCGGAGCAGG AAAACTTCGT CAAGCATTAA CCAACTGTGA TGACATCTTA 
ATCAAGCAGA ACCAGACCCT GGCTGAGCTG CAGAATAACA TGGTGCTAGT GAAACTGGAC 
CTTCAGAAAA AGGCAGCCTG CATTGCTGAG CAGTATCACA CTGTGCTAAA GCTCCAAGGC 
CAAGCCTCTG CCAAAAAGCG GCTGGGAGCA AACCAGGAAA ACCAGCAACC AAATCATCAA 
CCCCCAGGAA AGAAACCATT CCTGCGAAAC TTACTTCCCC GAACACCTAC CTGCCAAAGC 
TCAACAGACA GCAGCCCTTA TGCGCGGATC TTGCGCTCAC GGCACTCTCC TTTACTCAAA 
TCTCCCTTTG GCAAAAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif20a ( NM_009004.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033030.1 Mus musculus kinesin family member 20A (Kif20a), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu19128
Clone ID Related Accession (Same CDS sequence)	NM_001166407.1 , NM_009004.4 , NM_001166406.1
Accession Version	NM_009004.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF20A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY012551.1, AK144844.1 and AK084732.1. On Oct 22, 2009 this sequence version replaced NM_009004.3. Transcript Variant: This variant (3) has an alternate 5' UTR exon, as compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK084732.1, AK083412.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCACC GGATCCTTTC TCCGCCAGCG GGCTTACTCT CTGATGAGGA TGTTGTAGAC 
TCTCCCATAT TAGAATCCAC AGCTGCAGAT CTGAGATCTG TTGTCCGAAA GGACCTGTTG 
TCAGACTGCT CTGTCATCTC TGCCTCTCTG GAGGACAAGC AGGCTCTACT GGAGGACACC 
TCAGAGAAGG TGAAAGTTTA CCTTCGAATT CGACCATTCT TGACTTCAGA GTTGGACCGA 
CAGGAAGATC AGGGCTGCGT CTGCATTGAG AATACAGAAA CCCTTGTGCT GCAGGCACCC 
AAAGACTCCT TTGCCTTGAA GAGTAATGAG AGGGGAGTTG GTCAGGCCAC TCACAAATTT 
ACCTTTTCCC AGATATTTGG GCCAGAAGTG GGACAGGTGG CCTTCTTCAA TCTGACCATG 
AAGGAGATGG TGAAGGATGT GCTCAAAGGG CAGAACTGGC TCATCTACAC CTATGGAGTC 
ACCAACTCGG GGAAAACGTA CACCATTCAA GGTACTAGTA AGGATGCGGG GATCCTGCCC 
CAGTCCCTGG CTCTCATCTT TAATAGTCTC CAGGGCCAGC TTCATCCGAC ACCTGACCTG 
AAGCCCTTAT TGTCCAATGA AGTAATCTGG CTAGACAGCA AGCAGATTCG ACAGGAGGAA 
ATGAAGAAGC TGTCCTTGCT AATTGGAGGC CTCCAAGAGG AGGAGCTGTC CACCTCCGTG 
AAGAAACGTG TCCACACTGA AAGTCGGATA GGTGCCAGCA ACAGCTTTGA CAGTGGCGTT 
GCTGGGCTGT CTTCCACCAG TCAGTTCACC AGCAGCAGCC AGCTAGATGA AACAAGTCAA 
TTATGGGCAC AACCAGACAC TGTCCCAGTA AGTGTTCCAG CAGACATTCG CTTCTCTGTC 
TGGATCTCCT TCTTTGAGAT CTACAATGAA TTGCTTTATG ACCTGTTAGA ACCACCTAGC 
CATCAGCATA AGAGACAGAC ACTTCGGCTG TGTGAGGATC AGAACGGCAA TCCTTACGTG 
AAAGATCTCA ATTGGATTCA TGTTCGTGAT GTTGAGGAAG CCTGGAAACT ATTGAAAGTG 
GGTCGCAAGA ACCAGAGCTT TGCCAGCACC CACATGAACC AGCAATCCAG CCGCAGTCAC 
AGCATCTTCT CAATCAGAAT CTTGCACCTT CAGGGAGAAG GGGATATAGT GCCCAAGATC 
AGTGAACTGT CACTCTGTGA CCTGGCTGGC TCTGAGCGCT GCAAACATCA AAAAAGTGGT 
GAGCGGCTAA AGGAGGCAGG GAACATTAAC ACTTCTCTGC ACACCCTGGG CCGCTGTATT 
GCTGCCCTGC GACAGAATCA GCAGAACCGG TCAAAGCAGA ACCTGATTCC TTTCCGTGAC 
AGCAAGTTGA CTCGTGTGTT CCAAGGCTTC TTCACAGGTC GAGGTCGTTC CTGTATGATT 
GTCAATGTGA ATCCCTGTGC ATCTACATAT GACGAGACTC TTCATGCAGC CAAATTCTCA 
GCCCTAGCCA GCCAGCTTGT GCACGCCCCA CCTGTGCATC TGGGAATCCC ATCCCTGCAT 
TCATTCATCA AGAAGCACAG TCCTCAGGTT GGCCCTGGCT TAGAGAAAGA GGACAAGGCC 
GACTCAGACC TTGAGGACAG TCCTGAAGAC GAAGCTGACG TCTCCGTGTA TGGCAAGGAG 
GAACTACTCC AGGTGGTGGA AGCCATGAAA GCGCTACTTT TAAAAGAACG ACAAGAAAAG 
CTGCAGTTGG AGATACAGCT CCGAGAGGAA ATTTGCAACG AAATGGTGGA ACAGATGCAA 
CAGCGGGAGC AGTGGTGCAG TGAGCGTTTG GACAACCAAA AGGAACTGAT GGAGGAACTG 
TACGAGGAGA AACTGAAGAT CCTGAAGGAA TCACTGACAA CTTTCTACCA AGAGCAGATC 
CAGGAGCGGG ATGAAAAAAT TGAAGAGCTA GAAACTCTGT TGCAGGAAGC CAAACAGCAG 
CCAGCGGCAC AACAGTCAGG GGGGCTGTCC CTCCTCCGGC GGTCACAGAG GCTGGCAGCG 
TCTGCCTCCA CTCAGCAGTT CCAGGAAGTT AAAGCTGAAC TAGAGCAATG CAAAACAGAG 
TTAAGCTCTA CCACTGCAGA GCTGCACAAG TATCAGCAAG TGTTAAAACC GCCACCCCCA 
GCCAAGCCTT TCACCATTGA TGTGGATAAG AAGTTAGAGG AGGGCCAGAA GAATATAAGA 
CTGCTACGGA CAGAGCTCCA GAAACTAGGA CAGTCTCTGC AGTCAGCAGA AAGAGCCTGT 
TGCCACAGCA CCGGAGCAGG AAAACTTCGT CAAGCATTAA CCAACTGTGA TGACATCTTA 
ATCAAGCAGA ACCAGACCCT GGCTGAGCTG CAGAATAACA TGGTGCTAGT GAAACTGGAC 
CTTCAGAAAA AGGCAGCCTG CATTGCTGAG CAGTATCACA CTGTGCTAAA GCTCCAAGGC 
CAAGCCTCTG CCAAAAAGCG GCTGGGAGCA AACCAGGAAA ACCAGCAACC AAATCATCAA 
CCCCCAGGAA AGAAACCATT CCTGCGAAAC TTACTTCCCC GAACACCTAC CTGCCAAAGC 
TCAACAGACA GCAGCCCTTA TGCGCGGATC TTGCGCTCAC GGCACTCTCC TTTACTCAAA 
TCTCCCTTTG GCAAAAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033030.1
CDS	148..2811
Translation	MSHRILSPPAGLLSDEDVVDSPILESTAADLRSVVRKDLLSDCSVISASLEDKQALLEDTSEKVKVYLRIRPFLTSELDRQEDQGCVCIENTETLVLQAPKDSFALKSNERGVGQATHKFTFSQIFGPEVGQVAFFNLTMKEMVKDVLKGQNWLIYTYGVTNSGKTYTIQGTSKDAGILPQSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIRQEEMKKLSLLIGGLQEEELSTSVKKRVHTESRIGASNSFDSGVAGLSSTSQFTSSSQLDETSQLWAQPDTVPVSVPADIRFSVWISFFEIYNELLYDLLEPPSHQHKRQTLRLCEDQNGNPYVKDLNWIHVRDVEEAWKLLKVGRKNQSFASTHMNQQSSRSHSIFSIRILHLQGEGDIVPKISELSLCDLAGSERCKHQKSGERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLIPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHAAKFSALASQLVHAPPVHLGIPSLHSFIKKHSPQVGPGLEKEDKADSDLEDSPEDEADVSVYGKEELLQVVEAMKALLLKERQEKLQLEIQLREEICNEMVEQMQQREQWCSERLDNQKELMEELYEEKLKILKESLTTFYQEQIQERDEKIEELETLLQEAKQQPAAQQSGGLSLLRRSQRLAASASTQQFQEVKAELEQCKTELSSTTAELHKYQQVLKPPPPAKPFTIDVDKKLEEGQKNIRLLRTELQKLGQSLQSAERACCHSTGAGKLRQALTNCDDILIKQNQTLAELQNNMVLVKLDLQKKAACIAEQYHTVLKLQGQASAKKRLGANQENQQPNHQPPGKKPFLRNLLPRTPTCQSSTDSSPYARILRSRHSPLLKSPFGKKY

Target ORF information:

RefSeq Version	NM_009004.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 20A (Kif20a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009004.4

ORF Insert Sequence:

ATGTCTCACC GGATCCTTTC TCCGCCAGCG GGCTTACTCT CTGATGAGGA TGTTGTAGAC 
TCTCCCATAT TAGAATCCAC AGCTGCAGAT CTGAGATCTG TTGTCCGAAA GGACCTGTTG 
TCAGACTGCT CTGTCATCTC TGCCTCTCTG GAGGACAAGC AGGCTCTACT GGAGGACACC 
TCAGAGAAGG TGAAAGTTTA CCTTCGAATT CGACCATTCT TGACTTCAGA GTTGGACCGA 
CAGGAAGATC AGGGCTGCGT CTGCATTGAG AATACAGAAA CCCTTGTGCT GCAGGCACCC 
AAAGACTCCT TTGCCTTGAA GAGTAATGAG AGGGGAGTTG GTCAGGCCAC TCACAAATTT 
ACCTTTTCCC AGATATTTGG GCCAGAAGTG GGACAGGTGG CCTTCTTCAA TCTGACCATG 
AAGGAGATGG TGAAGGATGT GCTCAAAGGG CAGAACTGGC TCATCTACAC CTATGGAGTC 
ACCAACTCGG GGAAAACGTA CACCATTCAA GGTACTAGTA AGGATGCGGG GATCCTGCCC 
CAGTCCCTGG CTCTCATCTT TAATAGTCTC CAGGGCCAGC TTCATCCGAC ACCTGACCTG 
AAGCCCTTAT TGTCCAATGA AGTAATCTGG CTAGACAGCA AGCAGATTCG ACAGGAGGAA 
ATGAAGAAGC TGTCCTTGCT AATTGGAGGC CTCCAAGAGG AGGAGCTGTC CACCTCCGTG 
AAGAAACGTG TCCACACTGA AAGTCGGATA GGTGCCAGCA ACAGCTTTGA CAGTGGCGTT 
GCTGGGCTGT CTTCCACCAG TCAGTTCACC AGCAGCAGCC AGCTAGATGA AACAAGTCAA 
TTATGGGCAC AACCAGACAC TGTCCCAGTA AGTGTTCCAG CAGACATTCG CTTCTCTGTC 
TGGATCTCCT TCTTTGAGAT CTACAATGAA TTGCTTTATG ACCTGTTAGA ACCACCTAGC 
CATCAGCATA AGAGACAGAC ACTTCGGCTG TGTGAGGATC AGAACGGCAA TCCTTACGTG 
AAAGATCTCA ATTGGATTCA TGTTCGTGAT GTTGAGGAAG CCTGGAAACT ATTGAAAGTG 
GGTCGCAAGA ACCAGAGCTT TGCCAGCACC CACATGAACC AGCAATCCAG CCGCAGTCAC 
AGCATCTTCT CAATCAGAAT CTTGCACCTT CAGGGAGAAG GGGATATAGT GCCCAAGATC 
AGTGAACTGT CACTCTGTGA CCTGGCTGGC TCTGAGCGCT GCAAACATCA AAAAAGTGGT 
GAGCGGCTAA AGGAGGCAGG GAACATTAAC ACTTCTCTGC ACACCCTGGG CCGCTGTATT 
GCTGCCCTGC GACAGAATCA GCAGAACCGG TCAAAGCAGA ACCTGATTCC TTTCCGTGAC 
AGCAAGTTGA CTCGTGTGTT CCAAGGCTTC TTCACAGGTC GAGGTCGTTC CTGTATGATT 
GTCAATGTGA ATCCCTGTGC ATCTACATAT GACGAGACTC TTCATGCAGC CAAATTCTCA 
GCCCTAGCCA GCCAGCTTGT GCACGCCCCA CCTGTGCATC TGGGAATCCC ATCCCTGCAT 
TCATTCATCA AGAAGCACAG TCCTCAGGTT GGCCCTGGCT TAGAGAAAGA GGACAAGGCC 
GACTCAGACC TTGAGGACAG TCCTGAAGAC GAAGCTGACG TCTCCGTGTA TGGCAAGGAG 
GAACTACTCC AGGTGGTGGA AGCCATGAAA GCGCTACTTT TAAAAGAACG ACAAGAAAAG 
CTGCAGTTGG AGATACAGCT CCGAGAGGAA ATTTGCAACG AAATGGTGGA ACAGATGCAA 
CAGCGGGAGC AGTGGTGCAG TGAGCGTTTG GACAACCAAA AGGAACTGAT GGAGGAACTG 
TACGAGGAGA AACTGAAGAT CCTGAAGGAA TCACTGACAA CTTTCTACCA AGAGCAGATC 
CAGGAGCGGG ATGAAAAAAT TGAAGAGCTA GAAACTCTGT TGCAGGAAGC CAAACAGCAG 
CCAGCGGCAC AACAGTCAGG GGGGCTGTCC CTCCTCCGGC GGTCACAGAG GCTGGCAGCG 
TCTGCCTCCA CTCAGCAGTT CCAGGAAGTT AAAGCTGAAC TAGAGCAATG CAAAACAGAG 
TTAAGCTCTA CCACTGCAGA GCTGCACAAG TATCAGCAAG TGTTAAAACC GCCACCCCCA 
GCCAAGCCTT TCACCATTGA TGTGGATAAG AAGTTAGAGG AGGGCCAGAA GAATATAAGA 
CTGCTACGGA CAGAGCTCCA GAAACTAGGA CAGTCTCTGC AGTCAGCAGA AAGAGCCTGT 
TGCCACAGCA CCGGAGCAGG AAAACTTCGT CAAGCATTAA CCAACTGTGA TGACATCTTA 
ATCAAGCAGA ACCAGACCCT GGCTGAGCTG CAGAATAACA TGGTGCTAGT GAAACTGGAC 
CTTCAGAAAA AGGCAGCCTG CATTGCTGAG CAGTATCACA CTGTGCTAAA GCTCCAAGGC 
CAAGCCTCTG CCAAAAAGCG GCTGGGAGCA AACCAGGAAA ACCAGCAACC AAATCATCAA 
CCCCCAGGAA AGAAACCATT CCTGCGAAAC TTACTTCCCC GAACACCTAC CTGCCAAAGC 
TCAACAGACA GCAGCCCTTA TGCGCGGATC TTGCGCTCAC GGCACTCTCC TTTACTCAAA 
TCTCCCTTTG GCAAAAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif20a ( NM_001166406.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001159878.1 Mus musculus kinesin family member 20A (Kif20a), transcript variant 1, mRNA.

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CloneID	OMu19128
Clone ID Related Accession (Same CDS sequence)	NM_001166407.1 , NM_009004.4 , NM_001166406.1
Accession Version	NM_001166406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF20A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060608.1 and AK084732.1. Transcript Variant: This variant (1) and variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCACC GGATCCTTTC TCCGCCAGCG GGCTTACTCT CTGATGAGGA TGTTGTAGAC 
TCTCCCATAT TAGAATCCAC AGCTGCAGAT CTGAGATCTG TTGTCCGAAA GGACCTGTTG 
TCAGACTGCT CTGTCATCTC TGCCTCTCTG GAGGACAAGC AGGCTCTACT GGAGGACACC 
TCAGAGAAGG TGAAAGTTTA CCTTCGAATT CGACCATTCT TGACTTCAGA GTTGGACCGA 
CAGGAAGATC AGGGCTGCGT CTGCATTGAG AATACAGAAA CCCTTGTGCT GCAGGCACCC 
AAAGACTCCT TTGCCTTGAA GAGTAATGAG AGGGGAGTTG GTCAGGCCAC TCACAAATTT 
ACCTTTTCCC AGATATTTGG GCCAGAAGTG GGACAGGTGG CCTTCTTCAA TCTGACCATG 
AAGGAGATGG TGAAGGATGT GCTCAAAGGG CAGAACTGGC TCATCTACAC CTATGGAGTC 
ACCAACTCGG GGAAAACGTA CACCATTCAA GGTACTAGTA AGGATGCGGG GATCCTGCCC 
CAGTCCCTGG CTCTCATCTT TAATAGTCTC CAGGGCCAGC TTCATCCGAC ACCTGACCTG 
AAGCCCTTAT TGTCCAATGA AGTAATCTGG CTAGACAGCA AGCAGATTCG ACAGGAGGAA 
ATGAAGAAGC TGTCCTTGCT AATTGGAGGC CTCCAAGAGG AGGAGCTGTC CACCTCCGTG 
AAGAAACGTG TCCACACTGA AAGTCGGATA GGTGCCAGCA ACAGCTTTGA CAGTGGCGTT 
GCTGGGCTGT CTTCCACCAG TCAGTTCACC AGCAGCAGCC AGCTAGATGA AACAAGTCAA 
TTATGGGCAC AACCAGACAC TGTCCCAGTA AGTGTTCCAG CAGACATTCG CTTCTCTGTC 
TGGATCTCCT TCTTTGAGAT CTACAATGAA TTGCTTTATG ACCTGTTAGA ACCACCTAGC 
CATCAGCATA AGAGACAGAC ACTTCGGCTG TGTGAGGATC AGAACGGCAA TCCTTACGTG 
AAAGATCTCA ATTGGATTCA TGTTCGTGAT GTTGAGGAAG CCTGGAAACT ATTGAAAGTG 
GGTCGCAAGA ACCAGAGCTT TGCCAGCACC CACATGAACC AGCAATCCAG CCGCAGTCAC 
AGCATCTTCT CAATCAGAAT CTTGCACCTT CAGGGAGAAG GGGATATAGT GCCCAAGATC 
AGTGAACTGT CACTCTGTGA CCTGGCTGGC TCTGAGCGCT GCAAACATCA AAAAAGTGGT 
GAGCGGCTAA AGGAGGCAGG GAACATTAAC ACTTCTCTGC ACACCCTGGG CCGCTGTATT 
GCTGCCCTGC GACAGAATCA GCAGAACCGG TCAAAGCAGA ACCTGATTCC TTTCCGTGAC 
AGCAAGTTGA CTCGTGTGTT CCAAGGCTTC TTCACAGGTC GAGGTCGTTC CTGTATGATT 
GTCAATGTGA ATCCCTGTGC ATCTACATAT GACGAGACTC TTCATGCAGC CAAATTCTCA 
GCCCTAGCCA GCCAGCTTGT GCACGCCCCA CCTGTGCATC TGGGAATCCC ATCCCTGCAT 
TCATTCATCA AGAAGCACAG TCCTCAGGTT GGCCCTGGCT TAGAGAAAGA GGACAAGGCC 
GACTCAGACC TTGAGGACAG TCCTGAAGAC GAAGCTGACG TCTCCGTGTA TGGCAAGGAG 
GAACTACTCC AGGTGGTGGA AGCCATGAAA GCGCTACTTT TAAAAGAACG ACAAGAAAAG 
CTGCAGTTGG AGATACAGCT CCGAGAGGAA ATTTGCAACG AAATGGTGGA ACAGATGCAA 
CAGCGGGAGC AGTGGTGCAG TGAGCGTTTG GACAACCAAA AGGAACTGAT GGAGGAACTG 
TACGAGGAGA AACTGAAGAT CCTGAAGGAA TCACTGACAA CTTTCTACCA AGAGCAGATC 
CAGGAGCGGG ATGAAAAAAT TGAAGAGCTA GAAACTCTGT TGCAGGAAGC CAAACAGCAG 
CCAGCGGCAC AACAGTCAGG GGGGCTGTCC CTCCTCCGGC GGTCACAGAG GCTGGCAGCG 
TCTGCCTCCA CTCAGCAGTT CCAGGAAGTT AAAGCTGAAC TAGAGCAATG CAAAACAGAG 
TTAAGCTCTA CCACTGCAGA GCTGCACAAG TATCAGCAAG TGTTAAAACC GCCACCCCCA 
GCCAAGCCTT TCACCATTGA TGTGGATAAG AAGTTAGAGG AGGGCCAGAA GAATATAAGA 
CTGCTACGGA CAGAGCTCCA GAAACTAGGA CAGTCTCTGC AGTCAGCAGA AAGAGCCTGT 
TGCCACAGCA CCGGAGCAGG AAAACTTCGT CAAGCATTAA CCAACTGTGA TGACATCTTA 
ATCAAGCAGA ACCAGACCCT GGCTGAGCTG CAGAATAACA TGGTGCTAGT GAAACTGGAC 
CTTCAGAAAA AGGCAGCCTG CATTGCTGAG CAGTATCACA CTGTGCTAAA GCTCCAAGGC 
CAAGCCTCTG CCAAAAAGCG GCTGGGAGCA AACCAGGAAA ACCAGCAACC AAATCATCAA 
CCCCCAGGAA AGAAACCATT CCTGCGAAAC TTACTTCCCC GAACACCTAC CTGCCAAAGC 
TCAACAGACA GCAGCCCTTA TGCGCGGATC TTGCGCTCAC GGCACTCTCC TTTACTCAAA 
TCTCCCTTTG GCAAAAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159878.1
CDS	254..2917
Translation	MSHRILSPPAGLLSDEDVVDSPILESTAADLRSVVRKDLLSDCSVISASLEDKQALLEDTSEKVKVYLRIRPFLTSELDRQEDQGCVCIENTETLVLQAPKDSFALKSNERGVGQATHKFTFSQIFGPEVGQVAFFNLTMKEMVKDVLKGQNWLIYTYGVTNSGKTYTIQGTSKDAGILPQSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIRQEEMKKLSLLIGGLQEEELSTSVKKRVHTESRIGASNSFDSGVAGLSSTSQFTSSSQLDETSQLWAQPDTVPVSVPADIRFSVWISFFEIYNELLYDLLEPPSHQHKRQTLRLCEDQNGNPYVKDLNWIHVRDVEEAWKLLKVGRKNQSFASTHMNQQSSRSHSIFSIRILHLQGEGDIVPKISELSLCDLAGSERCKHQKSGERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLIPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHAAKFSALASQLVHAPPVHLGIPSLHSFIKKHSPQVGPGLEKEDKADSDLEDSPEDEADVSVYGKEELLQVVEAMKALLLKERQEKLQLEIQLREEICNEMVEQMQQREQWCSERLDNQKELMEELYEEKLKILKESLTTFYQEQIQERDEKIEELETLLQEAKQQPAAQQSGGLSLLRRSQRLAASASTQQFQEVKAELEQCKTELSSTTAELHKYQQVLKPPPPAKPFTIDVDKKLEEGQKNIRLLRTELQKLGQSLQSAERACCHSTGAGKLRQALTNCDDILIKQNQTLAELQNNMVLVKLDLQKKAACIAEQYHTVLKLQGQASAKKRLGANQENQQPNHQPPGKKPFLRNLLPRTPTCQSSTDSSPYARILRSRHSPLLKSPFGKKY

Target ORF information:

RefSeq Version	NM_001166406.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 20A (Kif20a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166406.1

ORF Insert Sequence:

ATGTCTCACC GGATCCTTTC TCCGCCAGCG GGCTTACTCT CTGATGAGGA TGTTGTAGAC 
TCTCCCATAT TAGAATCCAC AGCTGCAGAT CTGAGATCTG TTGTCCGAAA GGACCTGTTG 
TCAGACTGCT CTGTCATCTC TGCCTCTCTG GAGGACAAGC AGGCTCTACT GGAGGACACC 
TCAGAGAAGG TGAAAGTTTA CCTTCGAATT CGACCATTCT TGACTTCAGA GTTGGACCGA 
CAGGAAGATC AGGGCTGCGT CTGCATTGAG AATACAGAAA CCCTTGTGCT GCAGGCACCC 
AAAGACTCCT TTGCCTTGAA GAGTAATGAG AGGGGAGTTG GTCAGGCCAC TCACAAATTT 
ACCTTTTCCC AGATATTTGG GCCAGAAGTG GGACAGGTGG CCTTCTTCAA TCTGACCATG 
AAGGAGATGG TGAAGGATGT GCTCAAAGGG CAGAACTGGC TCATCTACAC CTATGGAGTC 
ACCAACTCGG GGAAAACGTA CACCATTCAA GGTACTAGTA AGGATGCGGG GATCCTGCCC 
CAGTCCCTGG CTCTCATCTT TAATAGTCTC CAGGGCCAGC TTCATCCGAC ACCTGACCTG 
AAGCCCTTAT TGTCCAATGA AGTAATCTGG CTAGACAGCA AGCAGATTCG ACAGGAGGAA 
ATGAAGAAGC TGTCCTTGCT AATTGGAGGC CTCCAAGAGG AGGAGCTGTC CACCTCCGTG 
AAGAAACGTG TCCACACTGA AAGTCGGATA GGTGCCAGCA ACAGCTTTGA CAGTGGCGTT 
GCTGGGCTGT CTTCCACCAG TCAGTTCACC AGCAGCAGCC AGCTAGATGA AACAAGTCAA 
TTATGGGCAC AACCAGACAC TGTCCCAGTA AGTGTTCCAG CAGACATTCG CTTCTCTGTC 
TGGATCTCCT TCTTTGAGAT CTACAATGAA TTGCTTTATG ACCTGTTAGA ACCACCTAGC 
CATCAGCATA AGAGACAGAC ACTTCGGCTG TGTGAGGATC AGAACGGCAA TCCTTACGTG 
AAAGATCTCA ATTGGATTCA TGTTCGTGAT GTTGAGGAAG CCTGGAAACT ATTGAAAGTG 
GGTCGCAAGA ACCAGAGCTT TGCCAGCACC CACATGAACC AGCAATCCAG CCGCAGTCAC 
AGCATCTTCT CAATCAGAAT CTTGCACCTT CAGGGAGAAG GGGATATAGT GCCCAAGATC 
AGTGAACTGT CACTCTGTGA CCTGGCTGGC TCTGAGCGCT GCAAACATCA AAAAAGTGGT 
GAGCGGCTAA AGGAGGCAGG GAACATTAAC ACTTCTCTGC ACACCCTGGG CCGCTGTATT 
GCTGCCCTGC GACAGAATCA GCAGAACCGG TCAAAGCAGA ACCTGATTCC TTTCCGTGAC 
AGCAAGTTGA CTCGTGTGTT CCAAGGCTTC TTCACAGGTC GAGGTCGTTC CTGTATGATT 
GTCAATGTGA ATCCCTGTGC ATCTACATAT GACGAGACTC TTCATGCAGC CAAATTCTCA 
GCCCTAGCCA GCCAGCTTGT GCACGCCCCA CCTGTGCATC TGGGAATCCC ATCCCTGCAT 
TCATTCATCA AGAAGCACAG TCCTCAGGTT GGCCCTGGCT TAGAGAAAGA GGACAAGGCC 
GACTCAGACC TTGAGGACAG TCCTGAAGAC GAAGCTGACG TCTCCGTGTA TGGCAAGGAG 
GAACTACTCC AGGTGGTGGA AGCCATGAAA GCGCTACTTT TAAAAGAACG ACAAGAAAAG 
CTGCAGTTGG AGATACAGCT CCGAGAGGAA ATTTGCAACG AAATGGTGGA ACAGATGCAA 
CAGCGGGAGC AGTGGTGCAG TGAGCGTTTG GACAACCAAA AGGAACTGAT GGAGGAACTG 
TACGAGGAGA AACTGAAGAT CCTGAAGGAA TCACTGACAA CTTTCTACCA AGAGCAGATC 
CAGGAGCGGG ATGAAAAAAT TGAAGAGCTA GAAACTCTGT TGCAGGAAGC CAAACAGCAG 
CCAGCGGCAC AACAGTCAGG GGGGCTGTCC CTCCTCCGGC GGTCACAGAG GCTGGCAGCG 
TCTGCCTCCA CTCAGCAGTT CCAGGAAGTT AAAGCTGAAC TAGAGCAATG CAAAACAGAG 
TTAAGCTCTA CCACTGCAGA GCTGCACAAG TATCAGCAAG TGTTAAAACC GCCACCCCCA 
GCCAAGCCTT TCACCATTGA TGTGGATAAG AAGTTAGAGG AGGGCCAGAA GAATATAAGA 
CTGCTACGGA CAGAGCTCCA GAAACTAGGA CAGTCTCTGC AGTCAGCAGA AAGAGCCTGT 
TGCCACAGCA CCGGAGCAGG AAAACTTCGT CAAGCATTAA CCAACTGTGA TGACATCTTA 
ATCAAGCAGA ACCAGACCCT GGCTGAGCTG CAGAATAACA TGGTGCTAGT GAAACTGGAC 
CTTCAGAAAA AGGCAGCCTG CATTGCTGAG CAGTATCACA CTGTGCTAAA GCTCCAAGGC 
CAAGCCTCTG CCAAAAAGCG GCTGGGAGCA AACCAGGAAA ACCAGCAACC AAATCATCAA 
CCCCCAGGAA AGAAACCATT CCTGCGAAAC TTACTTCCCC GAACACCTAC CTGCCAAAGC 
TCAACAGACA GCAGCCCTTA TGCGCGGATC TTGCGCTCAC GGCACTCTCC TTTACTCAAA 
TCTCCCTTTG GCAAAAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Developmental cell41(4)349-365.e3(2017 05)
Naiman N,Fujioka K,Fujino M,Valerius MT,Potter SS,McMahon AP,Kobayashi A

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

The vertebrate-specific Kinesin-6, Kif20b, is required for normal cytokinesis of polarized cortical stem cells and cerebral cortex size.
Development (Cambridge, England)140(23)4672-82(2013 Dec)
Janisch KM,Vock VM,Fleming MS,Shrestha A,Grimsley-Myers CM,Rasoul BA,Neale SA,Cupp TD,Kinchen JM,Liem KF Jr,Dwyer ND

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Kif20a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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