PYGM cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	PYGM
Entrez Gene ID	19309
Full Name	muscle glycogen phosphorylase
Synonyms	AI115133,PG
General protein information	Preferred Names muscle glycogen phosphorylase Names glycogen phosphorylase, muscle form myophosphorylase
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 4.53 cM
Summary	This gene encodes a glycolysis enzyme found in muscle. Highly similar enzymes encoded by different genes are found in liver and brain. The encoded protein is involved in regulating the breakdown of glycogen to glucose-1-phosphate, which is necessary for ATP generation. [provided by RefSeq, Dec 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_011224.2	NP_035354.1	glycogen phosphorylase, muscle form
NM_011224.1	NP_035354.1	glycogen phosphorylase, muscle form

Pathways from BioSystems

Homologies

	PYGM	XP_001114805.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007939	XP_317541.3
Oryza sativa (rice)	Os01g0851700	NP_001044823.1
Homo sapiens (human)	PYGM	NP_005600.1
Canis lupus familiaris (dog)	PYGM	XP_853123.1
Rattus norvegicus (Norway rat)	Pygm	NP_036770.1
Drosophila melanogaster (fruit fly)	GlyP	NP_722762.1
Xenopus tropicalis (tropical clawed frog)	LOC100491051	XP_002935410.2
Arabidopsis thaliana (thale cress)	PHS2	NP_190281.1
Bos taurus (cattle)	PYGM	NP_786980.1
Mus musculus (house mouse)	Pygm	NP_035354.1
Pan troglodytes (chimpanzee)	PYGM	XP_001165783.2
Danio rerio (zebrafish)	pygma	NP_001018464.1
Caenorhabditis elegans (roundworm)	T22F3.3	NP_504007.1

Gene Ontology
Bibliography

Related articles in PubMed

The glial phosphorylase of glycogen isoform is reduced in the dorsolateral prefrontal cortex in chronic schizophrenia.
Pinacho R, Vila E, Prades R, Tarrag? T, Castro E, Ferrer I, Ramos B
Schizophrenia research177(1-3)37-43(2016 11)

Differential glucose metabolism in mice and humans affected by McArdle disease.
Krag TO, Pin?s T, Nielsen TL, Duran J, Garc?a-Rocha M, Andreu AL, Vissing J
American journal of physiology. Regulatory, integrative and comparative physiology311(2)R307-14(2016 Aug)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that PYGM and RAC1 are altered in the dorsolateral prefrontal cortex in chronic schizophrenia and are controlled by NMDA signaling in the rodent cortex and cortical astrocytes suggesting an altered NMDA-dependent glycogenolysis in astrocytes in schizophrenia.
Title: The glial phosphorylase of glycogen isoform is reduced in the dorsolateral prefrontal cortex in chronic schizophrenia.

Data suggest that the irreversible inhibition of glycogen phosphorylase (GP) could represent one of the mechanisms that contribute to mercury-dependent muscle toxicity.
Title: Skeletal muscle glycogen phosphorylase is irreversibly inhibited by mercury: molecular, cellular and kinetic aspects.

this is the first successful attempt to develop a genetically modified animal model of McArdle disease.
Title: Knock-in mice for the R50X mutation in the PYGM gene present with McArdle disease.

Data show that glycogen phosphorylase is irreversibly impaired by exposure to peroxynitrite, and suggest that the peroxynitrite-dependent inactivation of the enzyme could be due to the nitration of Tyr613 at the allosteric inhibitor site of the enzyme.
Title: Nitration of a critical tyrosine residue in the allosteric inhibitor site of muscle glycogen phosphorylase impairs its catalytic activity.

The following PYGM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PYGM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13100	NM_011224.2 Latest version!	Mus musculus muscle glycogen phosphorylase (Pygm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$496.30 $709.00
View Old Accession Versions >>
OMu13100	NM_011224.1	Mus musculus muscle glycogen phosphorylase (Pygm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$496.30 $709.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PYGM ( NM_011224.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035354.1 Mus musculus muscle glycogen phosphorylase (Pygm), mRNA.

Price & Availability↑

CloneID	OMu13100
Clone ID Related Accession (Same CDS sequence)	NM_011224.2 , NM_011224.1
Accession Version	NM_011224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	glycogen phosphorylase, muscle form
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC167245.2 and AI849927.1. On Dec 1, 2015 this sequence version replaced NM_011224.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF124787.1, BC012961.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGGC CTCTTTCAGA CCAGGATAAG AGAAAGCAAA TCAGCGTTCG TGGCTTAGCC 
GGAGTGGAAA ATGTGTCGGA GCTAAAAAAG AACTTCAACC GCCACCTGCA TTTCACCTTG 
GTCAAGGATC GCAATGTGGC TACTCCGAGA GATTACTATT TTGCACTGGC CCACACTGTC 
CGGGACCACC TCGTGGGACG CTGGATCCGC ACACAGCAGC ATTACTACGA AAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTGGAGTTC TACATGGGCC GGACACTGCA GAACACCATG 
GTGAACCTGG CCTTGGAGAA CGCCTGTGAT GAGGCCACTT ACCAGCTGGG CTTGGACATG 
GAGGAGTTGG AGGAGATCGA GGAGGATGCA GGGTTGGGCA ATGGGGGCCT GGGACGCCTG 
GCAGCTTGCT TTTTGGACTC CATGGCCACC CTGGGGCTCG CTGCCTATGG CTATGGGATC 
CGCTATGAGT TTGGGATTTT TAATCAGAAG ATCTGTGGGG GCTGGCAGAT GGAAGAAGCT 
GATGACTGGC TTCGCTATGG CAACCCCTGG GAGAAGGCCC GCCCAGAGTT CACGCTGCCT 
GTGCACTTCT ATGGCCGAGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTAGTGCTGG CTATGCCCTA TGACACTCCT GTGCCCGGCT ATCGGAACAA CGTCGTCAAC 
ACCATGCGCC TCTGGTCGGC CAAGGCACCT AACGACTTCA ACCTCAAGGA CTTCAATGTT 
GGCGGCTACA TCCAGGCTGT GCTGGACCGA AACCTGGCCG AGAACATCTC GCGTGTTTTG 
TACCCCAATG ATAATTTCTT TGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGA CGTTTCAAGT CCTCCAAGTT TGGCAGCCGT 
GATCCTGTGC GCACAAACTT CGATGCCTTC CCTGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CTTTGGCCAT CCCAGAGCTG ATGAGGATTC TGGTGGACCT GGAACGACTG 
GACTGGGACA AGGCCTGGGA CGTAACAGTG AAGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCTGAAG CCCTGGAGCG CTGGCCAGTG CACCTCATGG AGACACTGCT GCCCCGCCAC 
TTGCAGATCA TTTACGAGAT CAACCAGCGT TTCCTTAACC GGGTGGCGGC TGCATTCCCT 
GGGGACGTAG ATAGGCTGCG GCGCATGTCA CTCGTGGAGG AAGGAGCAGT GAAACGCATC 
AACATGGCAC ACCTGTGCAT TGCTGGCTCG CACGCTGTCA ACGGCGTGGC TCGCATCCAT 
TCGGAGATCC TCAAGAAAAC CATCTTCAAG GACTTTTATG AGCTGGAGCC TCACAAGTTC 
CAGAACAAGA CCAATGGCAT CACTCCTCGG CGCTGGCTGG TTCTGTGTAA CCCTGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATTGGTGAG GACTACATCT CAGACCTGGA CCAGCTGCGC 
AAGCTGCTCT CCTATGTGGA TGATGAAGCC TTTATCCGTG ATGTGGCCAA AGTGAAACAG 
GAAAACAAGT TGAAGTTCTC TGCATACCTC GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCGCTCT TTGACGTCCA GGTGAAAAGG ATTCATGAGT ACAAGCGGCA GCTCCTCAAC 
TGCCTGCACA TCATCACCTT GTATAACCGC ATCAAAAGGG AGCCCAATAG GTTTATGGTG 
CCGAGGACTA TCATGATTGG AGGCAAGGCT GCACCCGGGT ACCACATGGC TAAGATGATC 
ATCAAGCTTA TCACTGCCAT TGGGGATGTG GTCAACCACG ACCCTGCGGT AGGAGACCGC 
CTCCGAGTGA TCTTCCTGGA GAACTACCGA GTCTCCCTTG CTGAGAAAGT TATTCCCGCC 
GCCGACCTCT CAGAGCAGAT CTCCACCGCG GGCACCGAGG CCTCGGGCAC TGGCAACATG 
AAGTTCATGC TCAATGGGGC TCTGACCATC GGCACCATGG ACGGTGCCAA CGTGGAGATG 
GCAGAGGAGG CGGGGGAGGA GAACTTCTTC ATCTTCGGCA TGCGAGTGGA GGACGTGGAA 
AGGCTGGACC AGAGAGGGTA CAATGCCCAG GAGTACTACG ACCGAATTCC TGAGCTTCGG 
CAGATCATCG AACAGCTAAG CAGCGGCTTC TTCTCCCCCA AGCAGCCTGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTTGCAGA TTATGAGGAA 
TACATTAAAT GCCAGGACAA AGTCAGTGAG TTGTACAAGA ACCCAAGAGA GTGGACACGG 
ATGGTGATCA GGAACATAGC CACCTCTGGC AAGTTTTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTTGAACCT TCTCGCCAGC GCCTGCCAGC CCCAGATGAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035354.1
CDS	72..2600
Translation	MSRPLSDQDKRKQISVRGLAGVENVSELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGSRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDLERLDWDKAWDVTVKTCAYTNHTVLPEALERWPVHLMETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEKI

Target ORF information:

RefSeq Version	NM_011224.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus muscle glycogen phosphorylase (Pygm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011224.2

ORF Insert Sequence:

ATGTCCAGGC CTCTTTCAGA CCAGGATAAG AGAAAGCAAA TCAGCGTTCG TGGCTTAGCC 
GGAGTGGAAA ATGTGTCGGA GCTAAAAAAG AACTTCAACC GCCACCTGCA TTTCACCTTG 
GTCAAGGATC GCAATGTGGC TACTCCGAGA GATTACTATT TTGCACTGGC CCACACTGTC 
CGGGACCACC TCGTGGGACG CTGGATCCGC ACACAGCAGC ATTACTACGA AAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTGGAGTTC TACATGGGCC GGACACTGCA GAACACCATG 
GTGAACCTGG CCTTGGAGAA CGCCTGTGAT GAGGCCACTT ACCAGCTGGG CTTGGACATG 
GAGGAGTTGG AGGAGATCGA GGAGGATGCA GGGTTGGGCA ATGGGGGCCT GGGACGCCTG 
GCAGCTTGCT TTTTGGACTC CATGGCCACC CTGGGGCTCG CTGCCTATGG CTATGGGATC 
CGCTATGAGT TTGGGATTTT TAATCAGAAG ATCTGTGGGG GCTGGCAGAT GGAAGAAGCT 
GATGACTGGC TTCGCTATGG CAACCCCTGG GAGAAGGCCC GCCCAGAGTT CACGCTGCCT 
GTGCACTTCT ATGGCCGAGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTAGTGCTGG CTATGCCCTA TGACACTCCT GTGCCCGGCT ATCGGAACAA CGTCGTCAAC 
ACCATGCGCC TCTGGTCGGC CAAGGCACCT AACGACTTCA ACCTCAAGGA CTTCAATGTT 
GGCGGCTACA TCCAGGCTGT GCTGGACCGA AACCTGGCCG AGAACATCTC GCGTGTTTTG 
TACCCCAATG ATAATTTCTT TGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGA CGTTTCAAGT CCTCCAAGTT TGGCAGCCGT 
GATCCTGTGC GCACAAACTT CGATGCCTTC CCTGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CTTTGGCCAT CCCAGAGCTG ATGAGGATTC TGGTGGACCT GGAACGACTG 
GACTGGGACA AGGCCTGGGA CGTAACAGTG AAGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCTGAAG CCCTGGAGCG CTGGCCAGTG CACCTCATGG AGACACTGCT GCCCCGCCAC 
TTGCAGATCA TTTACGAGAT CAACCAGCGT TTCCTTAACC GGGTGGCGGC TGCATTCCCT 
GGGGACGTAG ATAGGCTGCG GCGCATGTCA CTCGTGGAGG AAGGAGCAGT GAAACGCATC 
AACATGGCAC ACCTGTGCAT TGCTGGCTCG CACGCTGTCA ACGGCGTGGC TCGCATCCAT 
TCGGAGATCC TCAAGAAAAC CATCTTCAAG GACTTTTATG AGCTGGAGCC TCACAAGTTC 
CAGAACAAGA CCAATGGCAT CACTCCTCGG CGCTGGCTGG TTCTGTGTAA CCCTGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATTGGTGAG GACTACATCT CAGACCTGGA CCAGCTGCGC 
AAGCTGCTCT CCTATGTGGA TGATGAAGCC TTTATCCGTG ATGTGGCCAA AGTGAAACAG 
GAAAACAAGT TGAAGTTCTC TGCATACCTC GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCGCTCT TTGACGTCCA GGTGAAAAGG ATTCATGAGT ACAAGCGGCA GCTCCTCAAC 
TGCCTGCACA TCATCACCTT GTATAACCGC ATCAAAAGGG AGCCCAATAG GTTTATGGTG 
CCGAGGACTA TCATGATTGG AGGCAAGGCT GCACCCGGGT ACCACATGGC TAAGATGATC 
ATCAAGCTTA TCACTGCCAT TGGGGATGTG GTCAACCACG ACCCTGCGGT AGGAGACCGC 
CTCCGAGTGA TCTTCCTGGA GAACTACCGA GTCTCCCTTG CTGAGAAAGT TATTCCCGCC 
GCCGACCTCT CAGAGCAGAT CTCCACCGCG GGCACCGAGG CCTCGGGCAC TGGCAACATG 
AAGTTCATGC TCAATGGGGC TCTGACCATC GGCACCATGG ACGGTGCCAA CGTGGAGATG 
GCAGAGGAGG CGGGGGAGGA GAACTTCTTC ATCTTCGGCA TGCGAGTGGA GGACGTGGAA 
AGGCTGGACC AGAGAGGGTA CAATGCCCAG GAGTACTACG ACCGAATTCC TGAGCTTCGG 
CAGATCATCG AACAGCTAAG CAGCGGCTTC TTCTCCCCCA AGCAGCCTGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTTGCAGA TTATGAGGAA 
TACATTAAAT GCCAGGACAA AGTCAGTGAG TTGTACAAGA ACCCAAGAGA GTGGACACGG 
ATGGTGATCA GGAACATAGC CACCTCTGGC AAGTTTTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTTGAACCT TCTCGCCAGC GCCTGCCAGC CCCAGATGAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGM ( NM_011224.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035354.1 Mus musculus muscle glycogen phosphorylase (Pygm), mRNA.

Price & Availability↑

CloneID	OMu13100
Clone ID Related Accession (Same CDS sequence)	NM_011224.2 , NM_011224.1
Accession Version	NM_011224.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	glycogen phosphorylase, muscle form
Comment

ATGTCCAGGC CTCTTTCAGA CCAGGATAAG AGAAAGCAAA TCAGCGTTCG TGGCTTAGCC 
GGAGTGGAAA ATGTGTCGGA GCTAAAAAAG AACTTCAACC GCCACCTGCA TTTCACCTTG 
GTCAAGGATC GCAATGTGGC TACTCCGAGA GATTACTATT TTGCACTGGC CCACACTGTC 
CGGGACCACC TCGTGGGACG CTGGATCCGC ACACAGCAGC ATTACTACGA AAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTGGAGTTC TACATGGGCC GGACACTGCA GAACACCATG 
GTGAACCTGG CCTTGGAGAA CGCCTGTGAT GAGGCCACTT ACCAGCTGGG CTTGGACATG 
GAGGAGTTGG AGGAGATCGA GGAGGATGCA GGGTTGGGCA ATGGGGGCCT GGGACGCCTG 
GCAGCTTGCT TTTTGGACTC CATGGCCACC CTGGGGCTCG CTGCCTATGG CTATGGGATC 
CGCTATGAGT TTGGGATTTT TAATCAGAAG ATCTGTGGGG GCTGGCAGAT GGAAGAAGCT 
GATGACTGGC TTCGCTATGG CAACCCCTGG GAGAAGGCCC GCCCAGAGTT CACGCTGCCT 
GTGCACTTCT ATGGCCGAGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTAGTGCTGG CTATGCCCTA TGACACTCCT GTGCCCGGCT ATCGGAACAA CGTCGTCAAC 
ACCATGCGCC TCTGGTCGGC CAAGGCACCT AACGACTTCA ACCTCAAGGA CTTCAATGTT 
GGCGGCTACA TCCAGGCTGT GCTGGACCGA AACCTGGCCG AGAACATCTC GCGTGTTTTG 
TACCCCAATG ATAATTTCTT TGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGA CGTTTCAAGT CCTCCAAGTT TGGCAGCCGT 
GATCCTGTGC GCACAAACTT CGATGCCTTC CCTGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CTTTGGCCAT CCCAGAGCTG ATGAGGATTC TGGTGGACCT GGAACGACTG 
GACTGGGACA AGGCCTGGGA CGTAACAGTG AAGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCTGAAG CCCTGGAGCG CTGGCCAGTG CACCTCATGG AGACACTGCT GCCCCGCCAC 
TTGCAGATCA TTTACGAGAT CAACCAGCGT TTCCTTAACC GGGTGGCGGC TGCATTCCCT 
GGGGACGTAG ATAGGCTGCG GCGCATGTCA CTCGTGGAGG AAGGAGCAGT GAAACGCATC 
AACATGGCAC ACCTGTGCAT TGCTGGCTCG CACGCTGTCA ACGGCGTGGC TCGCATCCAT 
TCGGAGATCC TCAAGAAAAC CATCTTCAAG GACTTTTATG AGCTGGAGCC TCACAAGTTC 
CAGAACAAGA CCAATGGCAT CACTCCTCGG CGCTGGCTGG TTCTGTGTAA CCCTGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATTGGTGAG GACTACATCT CAGACCTGGA CCAGCTGCGC 
AAGCTGCTCT CCTATGTGGA TGATGAAGCC TTTATCCGTG ATGTGGCCAA AGTGAAACAG 
GAAAACAAGT TGAAGTTCTC TGCATACCTC GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCGCTCT TTGACGTCCA GGTGAAAAGG ATTCATGAGT ACAAGCGGCA GCTCCTCAAC 
TGCCTGCACA TCATCACCTT GTATAACCGC ATCAAAAGGG AGCCCAATAG GTTTATGGTG 
CCGAGGACTA TCATGATTGG AGGCAAGGCT GCACCCGGGT ACCACATGGC TAAGATGATC 
ATCAAGCTTA TCACTGCCAT TGGGGATGTG GTCAACCACG ACCCTGCGGT AGGAGACCGC 
CTCCGAGTGA TCTTCCTGGA GAACTACCGA GTCTCCCTTG CTGAGAAAGT TATTCCCGCC 
GCCGACCTCT CAGAGCAGAT CTCCACCGCG GGCACCGAGG CCTCGGGCAC TGGCAACATG 
AAGTTCATGC TCAATGGGGC TCTGACCATC GGCACCATGG ACGGTGCCAA CGTGGAGATG 
GCAGAGGAGG CGGGGGAGGA GAACTTCTTC ATCTTCGGCA TGCGAGTGGA GGACGTGGAA 
AGGCTGGACC AGAGAGGGTA CAATGCCCAG GAGTACTACG ACCGAATTCC TGAGCTTCGG 
CAGATCATCG AACAGCTAAG CAGCGGCTTC TTCTCCCCCA AGCAGCCTGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTTGCAGA TTATGAGGAA 
TACATTAAAT GCCAGGACAA AGTCAGTGAG TTGTACAAGA ACCCAAGAGA GTGGACACGG 
ATGGTGATCA GGAACATAGC CACCTCTGGC AAGTTTTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTTGAACCT TCTCGCCAGC GCCTGCCAGC CCCAGATGAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_035354.1

Translation

MSRPLSDQDKRKQISVRGLAGVENVSELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGSRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDLERLDWDKAWDVTVKTCAYTNHTVLPEALERWPVHLMETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEKI

Target ORF information:

RefSeq Version	NM_011224.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus muscle glycogen phosphorylase (Pygm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011224.1

ORF Insert Sequence:

ATGTCCAGGC CTCTTTCAGA CCAGGATAAG AGAAAGCAAA TCAGCGTTCG TGGCTTAGCC 
GGAGTGGAAA ATGTGTCGGA GCTAAAAAAG AACTTCAACC GCCACCTGCA TTTCACCTTG 
GTCAAGGATC GCAATGTGGC TACTCCGAGA GATTACTATT TTGCACTGGC CCACACTGTC 
CGGGACCACC TCGTGGGACG CTGGATCCGC ACACAGCAGC ATTACTACGA AAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTGGAGTTC TACATGGGCC GGACACTGCA GAACACCATG 
GTGAACCTGG CCTTGGAGAA CGCCTGTGAT GAGGCCACTT ACCAGCTGGG CTTGGACATG 
GAGGAGTTGG AGGAGATCGA GGAGGATGCA GGGTTGGGCA ATGGGGGCCT GGGACGCCTG 
GCAGCTTGCT TTTTGGACTC CATGGCCACC CTGGGGCTCG CTGCCTATGG CTATGGGATC 
CGCTATGAGT TTGGGATTTT TAATCAGAAG ATCTGTGGGG GCTGGCAGAT GGAAGAAGCT 
GATGACTGGC TTCGCTATGG CAACCCCTGG GAGAAGGCCC GCCCAGAGTT CACGCTGCCT 
GTGCACTTCT ATGGCCGAGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTAGTGCTGG CTATGCCCTA TGACACTCCT GTGCCCGGCT ATCGGAACAA CGTCGTCAAC 
ACCATGCGCC TCTGGTCGGC CAAGGCACCT AACGACTTCA ACCTCAAGGA CTTCAATGTT 
GGCGGCTACA TCCAGGCTGT GCTGGACCGA AACCTGGCCG AGAACATCTC GCGTGTTTTG 
TACCCCAATG ATAATTTCTT TGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGA CGTTTCAAGT CCTCCAAGTT TGGCAGCCGT 
GATCCTGTGC GCACAAACTT CGATGCCTTC CCTGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CTTTGGCCAT CCCAGAGCTG ATGAGGATTC TGGTGGACCT GGAACGACTG 
GACTGGGACA AGGCCTGGGA CGTAACAGTG AAGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCTGAAG CCCTGGAGCG CTGGCCAGTG CACCTCATGG AGACACTGCT GCCCCGCCAC 
TTGCAGATCA TTTACGAGAT CAACCAGCGT TTCCTTAACC GGGTGGCGGC TGCATTCCCT 
GGGGACGTAG ATAGGCTGCG GCGCATGTCA CTCGTGGAGG AAGGAGCAGT GAAACGCATC 
AACATGGCAC ACCTGTGCAT TGCTGGCTCG CACGCTGTCA ACGGCGTGGC TCGCATCCAT 
TCGGAGATCC TCAAGAAAAC CATCTTCAAG GACTTTTATG AGCTGGAGCC TCACAAGTTC 
CAGAACAAGA CCAATGGCAT CACTCCTCGG CGCTGGCTGG TTCTGTGTAA CCCTGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATTGGTGAG GACTACATCT CAGACCTGGA CCAGCTGCGC 
AAGCTGCTCT CCTATGTGGA TGATGAAGCC TTTATCCGTG ATGTGGCCAA AGTGAAACAG 
GAAAACAAGT TGAAGTTCTC TGCATACCTC GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCGCTCT TTGACGTCCA GGTGAAAAGG ATTCATGAGT ACAAGCGGCA GCTCCTCAAC 
TGCCTGCACA TCATCACCTT GTATAACCGC ATCAAAAGGG AGCCCAATAG GTTTATGGTG 
CCGAGGACTA TCATGATTGG AGGCAAGGCT GCACCCGGGT ACCACATGGC TAAGATGATC 
ATCAAGCTTA TCACTGCCAT TGGGGATGTG GTCAACCACG ACCCTGCGGT AGGAGACCGC 
CTCCGAGTGA TCTTCCTGGA GAACTACCGA GTCTCCCTTG CTGAGAAAGT TATTCCCGCC 
GCCGACCTCT CAGAGCAGAT CTCCACCGCG GGCACCGAGG CCTCGGGCAC TGGCAACATG 
AAGTTCATGC TCAATGGGGC TCTGACCATC GGCACCATGG ACGGTGCCAA CGTGGAGATG 
GCAGAGGAGG CGGGGGAGGA GAACTTCTTC ATCTTCGGCA TGCGAGTGGA GGACGTGGAA 
AGGCTGGACC AGAGAGGGTA CAATGCCCAG GAGTACTACG ACCGAATTCC TGAGCTTCGG 
CAGATCATCG AACAGCTAAG CAGCGGCTTC TTCTCCCCCA AGCAGCCTGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTTGCAGA TTATGAGGAA 
TACATTAAAT GCCAGGACAA AGTCAGTGAG TTGTACAAGA ACCCAAGAGA GTGGACACGG 
ATGGTGATCA GGAACATAGC CACCTCTGGC AAGTTTTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTTGAACCT TCTCGCCAGC GCCTGCCAGC CCCAGATGAG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The glial phosphorylase of glycogen isoform is reduced in the dorsolateral prefrontal cortex in chronic schizophrenia.
Schizophrenia research177(1-3)37-43(2016 11)
Pinacho R,Vila E,Prades R,Tarrag? T,Castro E,Ferrer I,Ramos B

Differential glucose metabolism in mice and humans affected by McArdle disease.
American journal of physiology. Regulatory, integrative and comparative physiology311(2)R307-14(2016 Aug)
Krag TO,Pin?s T,Nielsen TL,Duran J,Garc?a-Rocha M,Andreu AL,Vissing J

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

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PYGM cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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