Rapgef6 cDNA ORF clone, Mus musculus(house mouse)

The following Rapgef6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rapgef6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu19445 NM_001252498.1
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu19612 NM_001252497.1
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu45801 XM_006532565.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu45802 XM_006532566.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu45803 XM_006532567.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu45804 XM_006532568.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu85671 XM_030245698.1
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu45796 XM_006532560.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu45797 XM_006532561.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu45798 XM_006532562.3
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu45799 XM_006532563.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu45799 XM_030245697.1
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu45800 XM_006532564.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu77780 XM_017314348.2
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu19298 NM_175258.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu19412 NM_001252494.1
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu19500 NM_001252496.1
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OMu45802 XM_006532566.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu45800 XM_006532564.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu45798 XM_006532562.2 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu77780 XM_017314348.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu45803 XM_006532567.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu45804 XM_006532568.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu45797 XM_006532561.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu45796 XM_006532560.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu45799 XM_006532563.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu45801 XM_006532565.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu19445
    Clone ID Related Accession (Same CDS sequence) NM_001252498.1
    Accession Version NM_001252498.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-27
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK049669.1, AK164343.1 and AL607091.12. Transcript Variant: This variant (5) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting isoform (5) is shorter and has distinct N-and C-termini, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK049669.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAAGTAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001239427.1
    CDS905..2206
    Translation

    Target ORF information:

    RefSeq Version NM_001252498.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252498.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAAGTAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19612
    Clone ID Related Accession (Same CDS sequence) NM_001252497.1
    Accession Version NM_001252497.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-27
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB652674.1, AK164343.1, AK045540.1 and BB465827.1. Transcript Variant: This variant (4) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting isoform (4) is shorter and has distinct N-and C-termini, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK164343.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGGT
    AATGTTCCCA TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001239426.1
    CDS905..2719
    Translation

    Target ORF information:

    RefSeq Version NM_001252497.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252497.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGGT
    AATGTTCCCA TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45801
    Clone ID Related Accession (Same CDS sequence) XM_006532565.3 , XM_006532565.4
    Accession Version XM_006532565.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532565.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGCT
    GTTGGTTTTT ACTATATTCC TGATCAAGTT ATAAGAGTTT TCAAAGCAGA TCAACAAAGT
    TGCTACATTA TCATCAGTAA GGATACCACA GCTAAAGAAG TGGTCTGCCA AGCTGTACAG
    GAATTCGGTT TGACTGGTGC ATCTGACACA TACTCCCTCT GTGAAGTGTC TGTTACTCCT
    GAGGGTGTTA TAAAGCAGAG AAGACTTCCG GACCAGTTCT CCAAATTAGC TGATAGAATT
    CAACTCAATG GAAGGTATTA CCTAAAAAAC AACATGGAAA CAGAAACATT GTGTTCTGAT
    GAAGATGCTC AGGAACTACT TAAGGAAAGC CAGCTCTCCA TGCTGCAGCT CAGTACCATC
    GAGGTCGCCA CCCAGCTCTC CATGAGAGAC TTTGATTTAT TTCGTAACAT TGAGCCTACT
    GAATACATTG ATGATCTTTT TAAATTAGAT TCCAAAACAG GAAATACTCA CTTGAAACAA
    TTTGAGGATA TTGTAAACCA AGAGACATTC TGGGTTGCCT CAGAGATTTT AAGTGAGTCA
    AATCAGCTGA AAAGAATGAA GATTATTAAA CATTTTATCA AAATTGCTCT TCATTGCCGA
    GAATGCAAGA ATTTCAATTC CATGTTTGCA ATAATAAGTG GCTTGAACCT GGCACCGGTA
    GCACGACTCA GAGGCACTTG GGAAAAGTTG CCAAGCAAAT ATGAGAAACA TCTTCAAGAT
    CTACAAGACC TTTTTGATCC ATCTAGAAAT ATGGCAAAAT ACAGAAATAT TCTTAGTAGT
    CAAAGCATGC AGCCTCCAAT TATTCCACTC TTCCCTGTTG TCAAGAAAGA TATGACATTT
    CTCCATGAAG GAAATGACTC CAAAGTAGAT GGCTTAGTAA ACTTCGAGAA GCTAAGAATG
    ATTGCCAAGG AAATCCGTCA TATTATTCGA ATGACTTCTG CCAACATGGA CCCTGCCATG
    ATGTTCCGAC AGAGGAAGAA GAGGTGGCGG AGTCTGGGGT CATTGAGTCA AGGCAGCACA
    AATTCTAACA TGCTAGATGT CCAGGGAGGT GCTCACAAAA AAAGAGCACG CCGCAGCTCA
    CTGCTGAATG CCAAGAAACT GTATGAAGAT GCCCAAATGG CAAGGAAAGT GAAACAGTAT
    CTTTCTAGTC TGGACATAGA CACAGATGAG GAGAAGTTCC AGATGATGTC ACTGCAGTGG
    GAACCTGCAT ATGGTACCTT GACAAAGAAC TTAACTGAAA AAAGGTCAGC CAAATCATCT
    GAGATGTCTC CTGTACCATT GAGGTCAGTT GGCCAAACAG CTAAAGTCCA CTTGCATCAG
    CCACACAGAG TGAGCCAGGT GCTTCAGGTG CCAGCAGTTA ACTTGCACCC CATCCGGAAG
    AAAGGACAAG CCAAAGACCA TGTGCTGAGT ACAAGTTTAC CTCAGAAAGG TTTAGGACCA
    ACTGAGGAAG TGAGTGTTAA GAAACACACA GAAGACACTA TTTCTGTAGC GTCCTCTCTG
    CACTCTAGCC CTCCTGCATC TCCTCAGAAC TCCCCACGCA AAGGTTATAC ACTCACACCA
    TCAAGTAAAT GTGACAACTT GTCTGACTCC AGCCACAGTG AAATCTCCTC TCGGTCCAGC
    ATTGTGAGCA ATGGTTCTGT TGACTCCATG TCTGCAGCTG GGCAGGATGA ACGCTGTTCC
    TCCCACAGTC TGGCAGTTCC TGAGCCCACA GGGGCATTGG AAAAGACAGA TCACCCTTCC
    GGGATCTCAG ACCACAGTCA GCTTGCTCAC GGGTGGATGC TGTCAAAGCC GTGTCTTATC
    AAGGGTGTAG CTGTCTCATC TTCTCTGAGC AGCGAAGAGA TGTCTCATGA GCATGTTGTG
    CTGGAAGCAG CTGACAGTGG GCGGGGCAGT TGGACTTCCT GTTCCAGCAG CTCCCATGAC
    AACTTCCAAA GCCTTCAAAA TCAAAAAAGC TGGGACTTCC TGAATTCTTA CAGACACATG
    CATTTAGATG ACCCTATTGC AGAAGTTGAG CCCACTGACT GTGAGCCCTG TGCCTGTCCT
    AAAGGCTGCT CAAGAACTTG TGGCCAGTGC AAAGGCAGCC TAGAGACGAA CCAGCTGAGG
    CAGAGTTGGG CCTCCTCCAG CTCACTCTCG GACACATGTG AACCAAACTA CGGGACAGTT
    AAGCGGAGGG TGTTGGAAAG TGCACCAGCA GAGGCACCTG ATGGCTTGGA GCCCAGGGAT
    ACCACTGACC CTGTTTATAA AACTGTCACT TCCAGTACAG ACAAGGGCTT GATTGTGTAC
    TGTGTCACCT CACCCAAGAA AGGTGATAGG TATAGGGAGC CACCGCCCAC TCCTCCAGGA
    TATCTGGGAA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCCGCACCT AAAACCTCCA
    GACTATAGTG TGGCAGTACA GAGATCAAAG ATGATGCTTA ACAGCCTGTC TAGACTGCCG
    CCAGCTCCTC CCAGTAGCCA CACCTCGGCC TGGGTTCCCT CGAAGATTGG ATCCCAGCCT
    CAAAGGCATA GCCATCCCAA GCTAGCAGAT GTGGCTGATG CAGATAGCGA AGCAGATGAA
    AACGAGCAGG TGTCAGCAGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532628.1
    CDS7028..11431
    Translation

    Target ORF information:

    RefSeq Version XM_006532565.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532565.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGCT
    GTTGGTTTTT ACTATATTCC TGATCAAGTT ATAAGAGTTT TCAAAGCAGA TCAACAAAGT
    TGCTACATTA TCATCAGTAA GGATACCACA GCTAAAGAAG TGGTCTGCCA AGCTGTACAG
    GAATTCGGTT TGACTGGTGC ATCTGACACA TACTCCCTCT GTGAAGTGTC TGTTACTCCT
    GAGGGTGTTA TAAAGCAGAG AAGACTTCCG GACCAGTTCT CCAAATTAGC TGATAGAATT
    CAACTCAATG GAAGGTATTA CCTAAAAAAC AACATGGAAA CAGAAACATT GTGTTCTGAT
    GAAGATGCTC AGGAACTACT TAAGGAAAGC CAGCTCTCCA TGCTGCAGCT CAGTACCATC
    GAGGTCGCCA CCCAGCTCTC CATGAGAGAC TTTGATTTAT TTCGTAACAT TGAGCCTACT
    GAATACATTG ATGATCTTTT TAAATTAGAT TCCAAAACAG GAAATACTCA CTTGAAACAA
    TTTGAGGATA TTGTAAACCA AGAGACATTC TGGGTTGCCT CAGAGATTTT AAGTGAGTCA
    AATCAGCTGA AAAGAATGAA GATTATTAAA CATTTTATCA AAATTGCTCT TCATTGCCGA
    GAATGCAAGA ATTTCAATTC CATGTTTGCA ATAATAAGTG GCTTGAACCT GGCACCGGTA
    GCACGACTCA GAGGCACTTG GGAAAAGTTG CCAAGCAAAT ATGAGAAACA TCTTCAAGAT
    CTACAAGACC TTTTTGATCC ATCTAGAAAT ATGGCAAAAT ACAGAAATAT TCTTAGTAGT
    CAAAGCATGC AGCCTCCAAT TATTCCACTC TTCCCTGTTG TCAAGAAAGA TATGACATTT
    CTCCATGAAG GAAATGACTC CAAAGTAGAT GGCTTAGTAA ACTTCGAGAA GCTAAGAATG
    ATTGCCAAGG AAATCCGTCA TATTATTCGA ATGACTTCTG CCAACATGGA CCCTGCCATG
    ATGTTCCGAC AGAGGAAGAA GAGGTGGCGG AGTCTGGGGT CATTGAGTCA AGGCAGCACA
    AATTCTAACA TGCTAGATGT CCAGGGAGGT GCTCACAAAA AAAGAGCACG CCGCAGCTCA
    CTGCTGAATG CCAAGAAACT GTATGAAGAT GCCCAAATGG CAAGGAAAGT GAAACAGTAT
    CTTTCTAGTC TGGACATAGA CACAGATGAG GAGAAGTTCC AGATGATGTC ACTGCAGTGG
    GAACCTGCAT ATGGTACCTT GACAAAGAAC TTAACTGAAA AAAGGTCAGC CAAATCATCT
    GAGATGTCTC CTGTACCATT GAGGTCAGTT GGCCAAACAG CTAAAGTCCA CTTGCATCAG
    CCACACAGAG TGAGCCAGGT GCTTCAGGTG CCAGCAGTTA ACTTGCACCC CATCCGGAAG
    AAAGGACAAG CCAAAGACCA TGTGCTGAGT ACAAGTTTAC CTCAGAAAGG TTTAGGACCA
    ACTGAGGAAG TGAGTGTTAA GAAACACACA GAAGACACTA TTTCTGTAGC GTCCTCTCTG
    CACTCTAGCC CTCCTGCATC TCCTCAGAAC TCCCCACGCA AAGGTTATAC ACTCACACCA
    TCAAGTAAAT GTGACAACTT GTCTGACTCC AGCCACAGTG AAATCTCCTC TCGGTCCAGC
    ATTGTGAGCA ATGGTTCTGT TGACTCCATG TCTGCAGCTG GGCAGGATGA ACGCTGTTCC
    TCCCACAGTC TGGCAGTTCC TGAGCCCACA GGGGCATTGG AAAAGACAGA TCACCCTTCC
    GGGATCTCAG ACCACAGTCA GCTTGCTCAC GGGTGGATGC TGTCAAAGCC GTGTCTTATC
    AAGGGTGTAG CTGTCTCATC TTCTCTGAGC AGCGAAGAGA TGTCTCATGA GCATGTTGTG
    CTGGAAGCAG CTGACAGTGG GCGGGGCAGT TGGACTTCCT GTTCCAGCAG CTCCCATGAC
    AACTTCCAAA GCCTTCAAAA TCAAAAAAGC TGGGACTTCC TGAATTCTTA CAGACACATG
    CATTTAGATG ACCCTATTGC AGAAGTTGAG CCCACTGACT GTGAGCCCTG TGCCTGTCCT
    AAAGGCTGCT CAAGAACTTG TGGCCAGTGC AAAGGCAGCC TAGAGACGAA CCAGCTGAGG
    CAGAGTTGGG CCTCCTCCAG CTCACTCTCG GACACATGTG AACCAAACTA CGGGACAGTT
    AAGCGGAGGG TGTTGGAAAG TGCACCAGCA GAGGCACCTG ATGGCTTGGA GCCCAGGGAT
    ACCACTGACC CTGTTTATAA AACTGTCACT TCCAGTACAG ACAAGGGCTT GATTGTGTAC
    TGTGTCACCT CACCCAAGAA AGGTGATAGG TATAGGGAGC CACCGCCCAC TCCTCCAGGA
    TATCTGGGAA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCCGCACCT AAAACCTCCA
    GACTATAGTG TGGCAGTACA GAGATCAAAG ATGATGCTTA ACAGCCTGTC TAGACTGCCG
    CCAGCTCCTC CCAGTAGCCA CACCTCGGCC TGGGTTCCCT CGAAGATTGG ATCCCAGCCT
    CAAAGGCATA GCCATCCCAA GCTAGCAGAT GTGGCTGATG CAGATAGCGA AGCAGATGAA
    AACGAGCAGG TGTCAGCAGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45802
    Clone ID Related Accession (Same CDS sequence) XM_006532566.4 , XM_006532566.3
    Accession Version XM_006532566.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532566.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAAC AAAGTTGCTA CATTATCATC
    AGTAAGGATA CCACAGCTAA AGAAGTGGTC TGCCAAGCTG TACAGGAATT CGGTTTGACT
    GGTGCATCTG ACACATACTC CCTCTGTGAA GTGTCTGTTA CTCCTGAGGG TGTTATAAAG
    CAGAGAAGAC TTCCGGACCA GTTCTCCAAA TTAGCTGATA GAATTCAACT CAATGGAAGG
    TATTACCTAA AAAACAACAT GGAAACAGAA ACATTGTGTT CTGATGAAGA TGCTCAGGAA
    CTACTTAAGG AAAGCCAGCT CTCCATGCTG CAGCTCAGTA CCATCGAGGT CGCCACCCAG
    CTCTCCATGA GAGACTTTGA TTTATTTCGT AACATTGAGC CTACTGAATA CATTGATGAT
    CTTTTTAAAT TAGATTCCAA AACAGGAAAT ACTCACTTGA AACAATTTGA GGATATTGTA
    AACCAAGAGA CATTCTGGGT TGCCTCAGAG ATTTTAAGTG AGTCAAATCA GCTGAAAAGA
    ATGAAGATTA TTAAACATTT TATCAAAATT GCTCTTCATT GCCGAGAATG CAAGAATTTC
    AATTCCATGT TTGCAATAAT AAGTGGCTTG AACCTGGCAC CGGTAGCACG ACTCAGAGGC
    ACTTGGGAAA AGTTGCCAAG CAAATATGAG AAACATCTTC AAGATCTACA AGACCTTTTT
    GATCCATCTA GAAATATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTCCC TGTTGTCAAG AAAGATATGA CATTTCTCCA TGAAGGAAAT
    GACTCCAAAG TAGATGGCTT AGTAAACTTC GAGAAGCTAA GAATGATTGC CAAGGAAATC
    CGTCATATTA TTCGAATGAC TTCTGCCAAC ATGGACCCTG CCATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCATTG AGTCAAGGCA GCACAAATTC TAACATGCTA
    GATGTCCAGG GAGGTGCTCA CAAAAAAAGA GCACGCCGCA GCTCACTGCT GAATGCCAAG
    AAACTGTATG AAGATGCCCA AATGGCAAGG AAAGTGAAAC AGTATCTTTC TAGTCTGGAC
    ATAGACACAG ATGAGGAGAA GTTCCAGATG ATGTCACTGC AGTGGGAACC TGCATATGGT
    ACCTTGACAA AGAACTTAAC TGAAAAAAGG TCAGCCAAAT CATCTGAGAT GTCTCCTGTA
    CCATTGAGGT CAGTTGGCCA AACAGCTAAA GTCCACTTGC ATCAGCCACA CAGAGTGAGC
    CAGGTGCTTC AGGTGCCAGC AGTTAACTTG CACCCCATCC GGAAGAAAGG ACAAGCCAAA
    GACCATGTGC TGAGTACAAG TTTACCTCAG AAAGGTTTAG GACCAACTGA GGAAGTGAGT
    GTTAAGAAAC ACACAGAAGA CACTATTTCT GTAGCGTCCT CTCTGCACTC TAGCCCTCCT
    GCATCTCCTC AGAACTCCCC ACGCAAAGGT TATACACTCA CACCATCAAG TAAATGTGAC
    AACTTGTCTG ACTCCAGCCA CAGTGAAATC TCCTCTCGGT CCAGCATTGT GAGCAATGGT
    TCTGTTGACT CCATGTCTGC AGCTGGGCAG GATGAACGCT GTTCCTCCCA CAGTCTGGCA
    GTTCCTGAGC CCACAGGGGC ATTGGAAAAG ACAGATCACC CTTCCGGGAT CTCAGACCAC
    AGTCAGCTTG CTCACGGGTG GATGCTGTCA AAGCCGTGTC TTATCAAGGG TGTAGCTGTC
    TCATCTTCTC TGAGCAGCGA AGAGATGTCT CATGAGCATG TTGTGCTGGA AGCAGCTGAC
    AGTGGGCGGG GCAGTTGGAC TTCCTGTTCC AGCAGCTCCC ATGACAACTT CCAAAGCCTT
    CAAAATCAAA AAAGCTGGGA CTTCCTGAAT TCTTACAGAC ACATGCATTT AGATGACCCT
    ATTGCAGAAG TTGAGCCCAC TGACTGTGAG CCCTGTGCCT GTCCTAAAGG CTGCTCAAGA
    ACTTGTGGCC AGTGCAAAGG CAGCCTAGAG ACGAACCAGC TGAGGCAGAG TTGGGCCTCC
    TCCAGCTCAC TCTCGGACAC ATGTGAACCA AACTACGGGA CAGTTAAGCG GAGGGTGTTG
    GAAAGTGCAC CAGCAGAGGC ACCTGATGGC TTGGAGCCCA GGGATACCAC TGACCCTGTT
    TATAAAACTG TCACTTCCAG TACAGACAAG GGCTTGATTG TGTACTGTGT CACCTCACCC
    AAGAAAGGTG ATAGGTATAG GGAGCCACCG CCCACTCCTC CAGGATATCT GGGAATTTCT
    TTAGCGGACC TAAAGGAAGG ACCCCACCCG CACCTAAAAC CTCCAGACTA TAGTGTGGCA
    GTACAGAGAT CAAAGATGAT GCTTAACAGC CTGTCTAGAC TGCCGCCAGC TCCTCCCAGT
    AGCCACACCT CGGCCTGGGT TCCCTCGAAG ATTGGATCCC AGCCTCAAAG GCATAGCCAT
    CCCAAGCTAG CAGATGTGGC TGATGCAGAT AGCGAAGCAG ATGAAAACGA GCAGGTGTCA
    GCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532629.1
    CDS947..5335
    Translation

    Target ORF information:

    RefSeq Version XM_006532566.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532566.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAAC AAAGTTGCTA CATTATCATC
    AGTAAGGATA CCACAGCTAA AGAAGTGGTC TGCCAAGCTG TACAGGAATT CGGTTTGACT
    GGTGCATCTG ACACATACTC CCTCTGTGAA GTGTCTGTTA CTCCTGAGGG TGTTATAAAG
    CAGAGAAGAC TTCCGGACCA GTTCTCCAAA TTAGCTGATA GAATTCAACT CAATGGAAGG
    TATTACCTAA AAAACAACAT GGAAACAGAA ACATTGTGTT CTGATGAAGA TGCTCAGGAA
    CTACTTAAGG AAAGCCAGCT CTCCATGCTG CAGCTCAGTA CCATCGAGGT CGCCACCCAG
    CTCTCCATGA GAGACTTTGA TTTATTTCGT AACATTGAGC CTACTGAATA CATTGATGAT
    CTTTTTAAAT TAGATTCCAA AACAGGAAAT ACTCACTTGA AACAATTTGA GGATATTGTA
    AACCAAGAGA CATTCTGGGT TGCCTCAGAG ATTTTAAGTG AGTCAAATCA GCTGAAAAGA
    ATGAAGATTA TTAAACATTT TATCAAAATT GCTCTTCATT GCCGAGAATG CAAGAATTTC
    AATTCCATGT TTGCAATAAT AAGTGGCTTG AACCTGGCAC CGGTAGCACG ACTCAGAGGC
    ACTTGGGAAA AGTTGCCAAG CAAATATGAG AAACATCTTC AAGATCTACA AGACCTTTTT
    GATCCATCTA GAAATATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTCCC TGTTGTCAAG AAAGATATGA CATTTCTCCA TGAAGGAAAT
    GACTCCAAAG TAGATGGCTT AGTAAACTTC GAGAAGCTAA GAATGATTGC CAAGGAAATC
    CGTCATATTA TTCGAATGAC TTCTGCCAAC ATGGACCCTG CCATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCATTG AGTCAAGGCA GCACAAATTC TAACATGCTA
    GATGTCCAGG GAGGTGCTCA CAAAAAAAGA GCACGCCGCA GCTCACTGCT GAATGCCAAG
    AAACTGTATG AAGATGCCCA AATGGCAAGG AAAGTGAAAC AGTATCTTTC TAGTCTGGAC
    ATAGACACAG ATGAGGAGAA GTTCCAGATG ATGTCACTGC AGTGGGAACC TGCATATGGT
    ACCTTGACAA AGAACTTAAC TGAAAAAAGG TCAGCCAAAT CATCTGAGAT GTCTCCTGTA
    CCATTGAGGT CAGTTGGCCA AACAGCTAAA GTCCACTTGC ATCAGCCACA CAGAGTGAGC
    CAGGTGCTTC AGGTGCCAGC AGTTAACTTG CACCCCATCC GGAAGAAAGG ACAAGCCAAA
    GACCATGTGC TGAGTACAAG TTTACCTCAG AAAGGTTTAG GACCAACTGA GGAAGTGAGT
    GTTAAGAAAC ACACAGAAGA CACTATTTCT GTAGCGTCCT CTCTGCACTC TAGCCCTCCT
    GCATCTCCTC AGAACTCCCC ACGCAAAGGT TATACACTCA CACCATCAAG TAAATGTGAC
    AACTTGTCTG ACTCCAGCCA CAGTGAAATC TCCTCTCGGT CCAGCATTGT GAGCAATGGT
    TCTGTTGACT CCATGTCTGC AGCTGGGCAG GATGAACGCT GTTCCTCCCA CAGTCTGGCA
    GTTCCTGAGC CCACAGGGGC ATTGGAAAAG ACAGATCACC CTTCCGGGAT CTCAGACCAC
    AGTCAGCTTG CTCACGGGTG GATGCTGTCA AAGCCGTGTC TTATCAAGGG TGTAGCTGTC
    TCATCTTCTC TGAGCAGCGA AGAGATGTCT CATGAGCATG TTGTGCTGGA AGCAGCTGAC
    AGTGGGCGGG GCAGTTGGAC TTCCTGTTCC AGCAGCTCCC ATGACAACTT CCAAAGCCTT
    CAAAATCAAA AAAGCTGGGA CTTCCTGAAT TCTTACAGAC ACATGCATTT AGATGACCCT
    ATTGCAGAAG TTGAGCCCAC TGACTGTGAG CCCTGTGCCT GTCCTAAAGG CTGCTCAAGA
    ACTTGTGGCC AGTGCAAAGG CAGCCTAGAG ACGAACCAGC TGAGGCAGAG TTGGGCCTCC
    TCCAGCTCAC TCTCGGACAC ATGTGAACCA AACTACGGGA CAGTTAAGCG GAGGGTGTTG
    GAAAGTGCAC CAGCAGAGGC ACCTGATGGC TTGGAGCCCA GGGATACCAC TGACCCTGTT
    TATAAAACTG TCACTTCCAG TACAGACAAG GGCTTGATTG TGTACTGTGT CACCTCACCC
    AAGAAAGGTG ATAGGTATAG GGAGCCACCG CCCACTCCTC CAGGATATCT GGGAATTTCT
    TTAGCGGACC TAAAGGAAGG ACCCCACCCG CACCTAAAAC CTCCAGACTA TAGTGTGGCA
    GTACAGAGAT CAAAGATGAT GCTTAACAGC CTGTCTAGAC TGCCGCCAGC TCCTCCCAGT
    AGCCACACCT CGGCCTGGGT TCCCTCGAAG ATTGGATCCC AGCCTCAAAG GCATAGCCAT
    CCCAAGCTAG CAGATGTGGC TGATGCAGAT AGCGAAGCAG ATGAAAACGA GCAGGTGTCA
    GCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45803
    Clone ID Related Accession (Same CDS sequence) XM_006532567.4 , XM_006532567.3
    Accession Version XM_006532567.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532567.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGTTGGCA GTATCCTATC TGATCACAGC ATCCTGTTGC GTCTCTCAGG GTCTTCCTCA
    TCCCCTCCCC GTGAGCCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG
    GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACTGGGAGCA CAGCCAAAAG AACAGACAGG
    ACCAAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGGT GTGAGGACAC TACCAGGAGA
    AGGATGACAT CCCCGTTTAG AAGATTTAGG GAGAGAACGC TTTCAAGGGA GAGACTAGTC
    AACAACCAAA AAGAGGATGC ACATCACAAT CAAGCTACAG ACAGCTGTGA GAAAGTGAAG
    GATGTTGGAA GCAACATCAC AGATGAGAGA GGGAATGCCA TCTACGACTC GAGTAGCCAA
    GGCCACAGTA CCGCGCTCAG CTGCTTCTGT ACGCGGTTCA AGACTAAAAG GAGAAAGACT
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532630.1
    CDS206..4471
    Translation

    Target ORF information:

    RefSeq Version XM_006532567.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532567.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGTTGGCA GTATCCTATC TGATCACAGC ATCCTGTTGC GTCTCTCAGG GTCTTCCTCA
    TCCCCTCCCC GTGAGCCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG
    GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACTGGGAGCA CAGCCAAAAG AACAGACAGG
    ACCAAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGGT GTGAGGACAC TACCAGGAGA
    AGGATGACAT CCCCGTTTAG AAGATTTAGG GAGAGAACGC TTTCAAGGGA GAGACTAGTC
    AACAACCAAA AAGAGGATGC ACATCACAAT CAAGCTACAG ACAGCTGTGA GAAAGTGAAG
    GATGTTGGAA GCAACATCAC AGATGAGAGA GGGAATGCCA TCTACGACTC GAGTAGCCAA
    GGCCACAGTA CCGCGCTCAG CTGCTTCTGT ACGCGGTTCA AGACTAAAAG GAGAAAGACT
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45804
    Clone ID Related Accession (Same CDS sequence) XM_006532568.4 , XM_006532568.3
    Accession Version XM_006532568.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532568.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT
    GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA
    CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA
    GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC
    TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA
    TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG
    GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC
    CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT
    AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA
    TCTCCTCAGA ACTCCCCACG CAAAGTTGGC AGTATCCTAT CTGATCACAG CATCCTGTTG
    CGTCTCTCAG GGTCTTCCTC ATCCCCTCCC CGTGAGCCCA GCACCAAGAT CAGTGGCCAG
    AGCTGCCCCG GAATAGGTGG GGTTTACTTG CAAAAGAAAA TCCTTCAGAT TACTGGGAGC
    ACAGCCAAAA GAACAGACAG GACCAAGAAG GCCACTGAGG AGAATAGAGA CAGGACAAGG
    TGTGAGGACA CTACCAGGAG AAGGATGACA TCCCCGTTTA GAAGATTTAG GGAGAGAACG
    CTTTCAAGGG AGAGACTAGT CAACAACCAA AAAGAGGATG CACATCACAA TCAAGCTACA
    GACAGCTGTG AGAAAGTGAA GGATGTTGGA AGCAACATCA CAGATGAGAG AGGGAATGCC
    ATCTACGACT CGAGTAGCCA AGGCCACAGT ACCGCGCTCA GCTGCTTCTG TACGCGGTTC
    AAGACTAAAA GGAGAAAGAC TCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532631.1
    CDS206..4432
    Translation

    Target ORF information:

    RefSeq Version XM_006532568.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532568.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT
    GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA
    CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA
    GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC
    TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA
    TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG
    GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC
    CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT
    AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA
    TCTCCTCAGA ACTCCCCACG CAAAGTTGGC AGTATCCTAT CTGATCACAG CATCCTGTTG
    CGTCTCTCAG GGTCTTCCTC ATCCCCTCCC CGTGAGCCCA GCACCAAGAT CAGTGGCCAG
    AGCTGCCCCG GAATAGGTGG GGTTTACTTG CAAAAGAAAA TCCTTCAGAT TACTGGGAGC
    ACAGCCAAAA GAACAGACAG GACCAAGAAG GCCACTGAGG AGAATAGAGA CAGGACAAGG
    TGTGAGGACA CTACCAGGAG AAGGATGACA TCCCCGTTTA GAAGATTTAG GGAGAGAACG
    CTTTCAAGGG AGAGACTAGT CAACAACCAA AAAGAGGATG CACATCACAA TCAAGCTACA
    GACAGCTGTG AGAAAGTGAA GGATGTTGGA AGCAACATCA CAGATGAGAG AGGGAATGCC
    ATCTACGACT CGAGTAGCCA AGGCCACAGT ACCGCGCTCA GCTGCTTCTG TACGCGGTTC
    AAGACTAAAA GGAGAAAGAC TCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85671
    Clone ID Related Accession (Same CDS sequence) XM_030245698.1
    Accession Version XM_030245698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG TTGGCAGTAT CCTATCTGAT
    CACAGCATCC TGTTGCGTCT CTCAGGGTCT TCCTCATCCC CTCCCCGTGA GCCCAGCACC
    AAGATCAGTG GCCAGAGCTG CCCCGGAATA GGTGGGGTTT ACTTGCAAAA GAAAATCCTT
    CAGATTACTG GGAGCACAGC CAAAAGAACA GACAGGACCA AGAAGGCCAC TGAGGAGAAT
    AGAGACAGGA CAAGGTGTGA GGACACTACC AGGAGAAGGA TGACATCCCC GTTTAGAAGA
    TTTAGGGAGA GAACGCTTTC AAGGGAGAGA CTAGTCAACA ACCAAAAAGA GGATGCACAT
    CACAATCAAG CTACAGACAG CTGTGAGAAA GTGAAGGATG TTGGAAGCAA CATCACAGAT
    GAGAGAGGGA ATGCCATCTA CGACTCGAGT AGCCAAGGCC ACAGTACCGC GCTCAGCTGC
    TTCTGTACGC GGTTCAAGAC TAAAAGGAGA AAGACTCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101558.1
    CDS206..4447
    Translation

    Target ORF information:

    RefSeq Version XM_030245698.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG TTGGCAGTAT CCTATCTGAT
    CACAGCATCC TGTTGCGTCT CTCAGGGTCT TCCTCATCCC CTCCCCGTGA GCCCAGCACC
    AAGATCAGTG GCCAGAGCTG CCCCGGAATA GGTGGGGTTT ACTTGCAAAA GAAAATCCTT
    CAGATTACTG GGAGCACAGC CAAAAGAACA GACAGGACCA AGAAGGCCAC TGAGGAGAAT
    AGAGACAGGA CAAGGTGTGA GGACACTACC AGGAGAAGGA TGACATCCCC GTTTAGAAGA
    TTTAGGGAGA GAACGCTTTC AAGGGAGAGA CTAGTCAACA ACCAAAAAGA GGATGCACAT
    CACAATCAAG CTACAGACAG CTGTGAGAAA GTGAAGGATG TTGGAAGCAA CATCACAGAT
    GAGAGAGGGA ATGCCATCTA CGACTCGAGT AGCCAAGGCC ACAGTACCGC GCTCAGCTGC
    TTCTGTACGC GGTTCAAGAC TAAAAGGAGA AAGACTCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45796
    Clone ID Related Accession (Same CDS sequence) XM_006532560.4 , XM_006532560.3
    Accession Version XM_006532560.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532560.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT
    GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT
    GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG
    GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG
    CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG
    ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC
    TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC
    CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC
    TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC
    CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT
    GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT
    GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA
    GACAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AAGGTGATAG GTATAGGGAG
    CCACCGCCCA CTCCTCCAGG ATATCTGGGA ATTTCTTTAG CGGACCTAAA GGAAGGACCC
    CACCCGCACC TAAAACCTCC AGACTATAGT GTGGCAGTAC AGAGATCAAA GATGATGCTT
    AACAGCCTGT CTAGACTGCC GCCAGCTCCT CCCAGTAGCC ACACCTCGGC CTGGGTTCCC
    TCGAAGATTG GATCCCAGCC TCAAAGGCAT AGCCATCCCA AGCTAGCAGA TGTGGCTGAT
    GCAGATAGCG AAGCAGATGA AAACGAGCAG GTGTCAGCAG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532623.1
    CDS242..5086
    Translation

    Target ORF information:

    RefSeq Version XM_006532560.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532560.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT
    GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT
    GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG
    GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG
    CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG
    ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC
    TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC
    CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC
    TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC
    CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT
    GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT
    GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA
    GACAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AAGGTGATAG GTATAGGGAG
    CCACCGCCCA CTCCTCCAGG ATATCTGGGA ATTTCTTTAG CGGACCTAAA GGAAGGACCC
    CACCCGCACC TAAAACCTCC AGACTATAGT GTGGCAGTAC AGAGATCAAA GATGATGCTT
    AACAGCCTGT CTAGACTGCC GCCAGCTCCT CCCAGTAGCC ACACCTCGGC CTGGGTTCCC
    TCGAAGATTG GATCCCAGCC TCAAAGGCAT AGCCATCCCA AGCTAGCAGA TGTGGCTGAT
    GCAGATAGCG AAGCAGATGA AAACGAGCAG GTGTCAGCAG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45797
    Clone ID Related Accession (Same CDS sequence) XM_006532561.3 , XM_006532561.4
    Accession Version XM_006532561.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532561.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC
    AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC
    AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA
    CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG
    CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA
    TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG
    CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC
    CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA
    GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC
    TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC
    ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG
    TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC
    TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC
    CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT
    CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT
    GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC
    CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA
    CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC
    TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG
    CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG
    ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC
    AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT
    GTGTACTGTG TCACCTCACC CAAGAAAGGT GATAGGTATA GGGAGCCACC GCCCACTCCT
    CCAGGATATC TGGGAATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCC GCACCTAAAA
    CCTCCAGACT ATAGTGTGGC AGTACAGAGA TCAAAGATGA TGCTTAACAG CCTGTCTAGA
    CTGCCGCCAG CTCCTCCCAG TAGCCACACC TCGGCCTGGG TTCCCTCGAA GATTGGATCC
    CAGCCTCAAA GGCATAGCCA TCCCAAGCTA GCAGATGTGG CTGATGCAGA TAGCGAAGCA
    GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532624.1
    CDS206..5035
    Translation

    Target ORF information:

    RefSeq Version XM_006532561.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532561.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC
    AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC
    AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA
    CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG
    CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA
    TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG
    CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC
    CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA
    GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC
    TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC
    ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG
    TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC
    TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC
    CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT
    CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT
    GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC
    CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA
    CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC
    TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG
    CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG
    ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC
    AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT
    GTGTACTGTG TCACCTCACC CAAGAAAGGT GATAGGTATA GGGAGCCACC GCCCACTCCT
    CCAGGATATC TGGGAATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCC GCACCTAAAA
    CCTCCAGACT ATAGTGTGGC AGTACAGAGA TCAAAGATGA TGCTTAACAG CCTGTCTAGA
    CTGCCGCCAG CTCCTCCCAG TAGCCACACC TCGGCCTGGG TTCCCTCGAA GATTGGATCC
    CAGCCTCAAA GGCATAGCCA TCCCAAGCTA GCAGATGTGG CTGATGCAGA TAGCGAAGCA
    GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45798
    Clone ID Related Accession (Same CDS sequence) XM_006532562.3 , XM_006532562.2
    Accession Version XM_006532562.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532562.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTACAA GAGCACGCTA TGAGAGATAC AGTGGCAATC AAATGCTTTT TTGTTCAGAA 
    ACTATTGCCA GATGTTGGTA TATCCTGCTT TCTGGATCTG TACTTGTAAA AGATTCTATG
    GTCCTTCCTC CTTGCAGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT
    GTGTTAGAAC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGACAGTATT
    TTACAAAGAG AAATTCCTGC CAGACAGTCT CGACGAAGGT TTCGGAAAAT TAACTATAAA
    GGAGAACGCC AAACCATAAT AGATGGAGTG GATATTAATA ACTACCTTTC GCTTCCAGCT
    GATCTTACCA AGATGCACCT CACAGACAAC CCTCATCCAC AGGTGACGCA TGTGTCTTCT
    AGTCAGTCTG GCTGTAGCAT TGCCAGTGAC TCTGGGAGCA GCAGTTTATC CGATATCTAT
    CAGGCTACAG AGAGTGAAGT AGGAGATGTA GATTTAACAC GCCTTCCAGA AGGACCTGTT
    GATTCTGAGG ATGAAGAGGA GGAGGAAGAA GAGATTGATA GAACAGATCC ACTGCAGGGT
    CGAGATCTTG TTCGGGAGTG TCTTGAAAAG GAACCCGCAG ACAAAACTGA TGATGATGTT
    GAACAGTTAC TTGAGTTCAT GCACCAGCTG CCTGCCTTTG CGAACATGAC CATGTCTGTA
    AGGAGAGAAC TCTGCTCCGT CATGGTTTTT GAAGTAGTAG AGCAGGCTGG GGCTGTTATT
    CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTCATTT TAAATGGCAC TGTAGAAATT
    AGTCATCCAG ATGGGAAAAT AGAAAATCTC TTCATGGGAA ATAGTTTTGG GATTGTCCCC
    ACGCTGGATA AGCAGCACAT GCATGGAGCT GTCAGGACCA AAGTCGATGA TTGTCAGTTT
    GTCTGCATAG CTCAACAAGA CTATTGGAGA ATTTTAAATC ATGTGGAAAA AAATACTCAC
    AAAGTTGAGG AAGAGGGAGA GATTGTCATG GTACATGAGC ACCGTGAGCT AGACCGGAGC
    GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTG
    ATAGAAGAAC ATTCTATTGT GGACCCAACA TACATAGAAG ATTTCCTCTT AACTTACAGG
    ACATTTCTTG AAACTCCTTT GGATGTTGGG ATCAAGCTTT TGGAATGGTT TAAGATTGAC
    AACCTAAGGG ATAAGGTGAC ACGGATTGTA CTCTTATGGG TAAATAATCA TTTTAATGAT
    TTTGAAGGTG ATCCTGCTAT GACTCAATTT CTAGAAGAAT TTGAAAGAAA TTTGGAAGAT
    ACAAAAATGA ATGGTCACCT CAGGTTATTG AATATTGCCT GTGCTGCAAA GGCTAAGTGG
    AGGCAAGTTG TACTGCAGAA GGCGTCCCGT GAGTCCCCGC TGCACTTCTG CCTTACTGGG
    GGCAGTGAGA AGGGATTTGG AGTCTTTGTT GAAGAAGTAG AATCTGGTAG CAAAGCTGCT
    GACGCAGGAC TGAAAAGGGG TGATCAGGTA ATGGAAGTCA ATGGACAAAA CTTTGAGAAT
    ATTACACTTG CTAAAGCCCT TGAAATTTTG AGAAATAATA CTCATCTTGC ACTTACTGTG
    AAGACCAACA TTTTTGTGTT CAAAGAATTG CTCAGTAGGA CTGAACAAGA GAAATCTGGT
    GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT TCAAGATGTC
    CCAGGGGATA TGGAGCAGGC ACCACAGGAG AAAGGAAATA AGAAAATTAA AGCAAATACC
    GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG
    CCTCCAAAAC TATTTAGTGA CGGAGGCCTG AGTCAATCAC AAGATGACAG CATTGTGGGA
    ACCAGGCACT GTAGGCACAG CCTGGCTATA ATGCCAATTC CCGGAACACT TTCATCCAGC
    AGCCCAGATC TCTTACAGCC TACCACTAGC ATGTTGGACT TTTCCAATCC TTCAGCTGTT
    GGTTTTTACT ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC
    TACATTATCA TCAGTAAGGA TACCACAGCT AAAGAAGTGG TCTGCCAAGC TGTACAGGAA
    TTCGGTTTGA CTGGTGCATC TGACACATAC TCCCTCTGTG AAGTGTCTGT TACTCCTGAG
    GGTGTTATAA AGCAGAGAAG ACTTCCGGAC CAGTTCTCCA AATTAGCTGA TAGAATTCAA
    CTCAATGGAA GGTATTACCT AAAAAACAAC ATGGAAACAG AAACATTGTG TTCTGATGAA
    GATGCTCAGG AACTACTTAA GGAAAGCCAG CTCTCCATGC TGCAGCTCAG TACCATCGAG
    GTCGCCACCC AGCTCTCCAT GAGAGACTTT GATTTATTTC GTAACATTGA GCCTACTGAA
    TACATTGATG ATCTTTTTAA ATTAGATTCC AAAACAGGAA ATACTCACTT GAAACAATTT
    GAGGATATTG TAAACCAAGA GACATTCTGG GTTGCCTCAG AGATTTTAAG TGAGTCAAAT
    CAGCTGAAAA GAATGAAGAT TATTAAACAT TTTATCAAAA TTGCTCTTCA TTGCCGAGAA
    TGCAAGAATT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ACCGGTAGCA
    CGACTCAGAG GCACTTGGGA AAAGTTGCCA AGCAAATATG AGAAACATCT TCAAGATCTA
    CAAGACCTTT TTGATCCATC TAGAAATATG GCAAAATACA GAAATATTCT TAGTAGTCAA
    AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTC
    CATGAAGGAA ATGACTCCAA AGTAGATGGC TTAGTAAACT TCGAGAAGCT AAGAATGATT
    GCCAAGGAAA TCCGTCATAT TATTCGAATG ACTTCTGCCA ACATGGACCC TGCCATGATG
    TTCCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT
    GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT
    CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC
    TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA
    GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA
    AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC
    GAGCAGGTGT CAGCAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532625.1
    CDS234..4934
    Translation

    Target ORF information:

    RefSeq Version XM_006532562.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532562.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTACAA GAGCACGCTA TGAGAGATAC AGTGGCAATC AAATGCTTTT TTGTTCAGAA 
    ACTATTGCCA GATGTTGGTA TATCCTGCTT TCTGGATCTG TACTTGTAAA AGATTCTATG
    GTCCTTCCTC CTTGCAGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT
    GTGTTAGAAC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGACAGTATT
    TTACAAAGAG AAATTCCTGC CAGACAGTCT CGACGAAGGT TTCGGAAAAT TAACTATAAA
    GGAGAACGCC AAACCATAAT AGATGGAGTG GATATTAATA ACTACCTTTC GCTTCCAGCT
    GATCTTACCA AGATGCACCT CACAGACAAC CCTCATCCAC AGGTGACGCA TGTGTCTTCT
    AGTCAGTCTG GCTGTAGCAT TGCCAGTGAC TCTGGGAGCA GCAGTTTATC CGATATCTAT
    CAGGCTACAG AGAGTGAAGT AGGAGATGTA GATTTAACAC GCCTTCCAGA AGGACCTGTT
    GATTCTGAGG ATGAAGAGGA GGAGGAAGAA GAGATTGATA GAACAGATCC ACTGCAGGGT
    CGAGATCTTG TTCGGGAGTG TCTTGAAAAG GAACCCGCAG ACAAAACTGA TGATGATGTT
    GAACAGTTAC TTGAGTTCAT GCACCAGCTG CCTGCCTTTG CGAACATGAC CATGTCTGTA
    AGGAGAGAAC TCTGCTCCGT CATGGTTTTT GAAGTAGTAG AGCAGGCTGG GGCTGTTATT
    CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTCATTT TAAATGGCAC TGTAGAAATT
    AGTCATCCAG ATGGGAAAAT AGAAAATCTC TTCATGGGAA ATAGTTTTGG GATTGTCCCC
    ACGCTGGATA AGCAGCACAT GCATGGAGCT GTCAGGACCA AAGTCGATGA TTGTCAGTTT
    GTCTGCATAG CTCAACAAGA CTATTGGAGA ATTTTAAATC ATGTGGAAAA AAATACTCAC
    AAAGTTGAGG AAGAGGGAGA GATTGTCATG GTACATGAGC ACCGTGAGCT AGACCGGAGC
    GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTG
    ATAGAAGAAC ATTCTATTGT GGACCCAACA TACATAGAAG ATTTCCTCTT AACTTACAGG
    ACATTTCTTG AAACTCCTTT GGATGTTGGG ATCAAGCTTT TGGAATGGTT TAAGATTGAC
    AACCTAAGGG ATAAGGTGAC ACGGATTGTA CTCTTATGGG TAAATAATCA TTTTAATGAT
    TTTGAAGGTG ATCCTGCTAT GACTCAATTT CTAGAAGAAT TTGAAAGAAA TTTGGAAGAT
    ACAAAAATGA ATGGTCACCT CAGGTTATTG AATATTGCCT GTGCTGCAAA GGCTAAGTGG
    AGGCAAGTTG TACTGCAGAA GGCGTCCCGT GAGTCCCCGC TGCACTTCTG CCTTACTGGG
    GGCAGTGAGA AGGGATTTGG AGTCTTTGTT GAAGAAGTAG AATCTGGTAG CAAAGCTGCT
    GACGCAGGAC TGAAAAGGGG TGATCAGGTA ATGGAAGTCA ATGGACAAAA CTTTGAGAAT
    ATTACACTTG CTAAAGCCCT TGAAATTTTG AGAAATAATA CTCATCTTGC ACTTACTGTG
    AAGACCAACA TTTTTGTGTT CAAAGAATTG CTCAGTAGGA CTGAACAAGA GAAATCTGGT
    GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT TCAAGATGTC
    CCAGGGGATA TGGAGCAGGC ACCACAGGAG AAAGGAAATA AGAAAATTAA AGCAAATACC
    GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG
    CCTCCAAAAC TATTTAGTGA CGGAGGCCTG AGTCAATCAC AAGATGACAG CATTGTGGGA
    ACCAGGCACT GTAGGCACAG CCTGGCTATA ATGCCAATTC CCGGAACACT TTCATCCAGC
    AGCCCAGATC TCTTACAGCC TACCACTAGC ATGTTGGACT TTTCCAATCC TTCAGCTGTT
    GGTTTTTACT ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC
    TACATTATCA TCAGTAAGGA TACCACAGCT AAAGAAGTGG TCTGCCAAGC TGTACAGGAA
    TTCGGTTTGA CTGGTGCATC TGACACATAC TCCCTCTGTG AAGTGTCTGT TACTCCTGAG
    GGTGTTATAA AGCAGAGAAG ACTTCCGGAC CAGTTCTCCA AATTAGCTGA TAGAATTCAA
    CTCAATGGAA GGTATTACCT AAAAAACAAC ATGGAAACAG AAACATTGTG TTCTGATGAA
    GATGCTCAGG AACTACTTAA GGAAAGCCAG CTCTCCATGC TGCAGCTCAG TACCATCGAG
    GTCGCCACCC AGCTCTCCAT GAGAGACTTT GATTTATTTC GTAACATTGA GCCTACTGAA
    TACATTGATG ATCTTTTTAA ATTAGATTCC AAAACAGGAA ATACTCACTT GAAACAATTT
    GAGGATATTG TAAACCAAGA GACATTCTGG GTTGCCTCAG AGATTTTAAG TGAGTCAAAT
    CAGCTGAAAA GAATGAAGAT TATTAAACAT TTTATCAAAA TTGCTCTTCA TTGCCGAGAA
    TGCAAGAATT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ACCGGTAGCA
    CGACTCAGAG GCACTTGGGA AAAGTTGCCA AGCAAATATG AGAAACATCT TCAAGATCTA
    CAAGACCTTT TTGATCCATC TAGAAATATG GCAAAATACA GAAATATTCT TAGTAGTCAA
    AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTC
    CATGAAGGAA ATGACTCCAA AGTAGATGGC TTAGTAAACT TCGAGAAGCT AAGAATGATT
    GCCAAGGAAA TCCGTCATAT TATTCGAATG ACTTCTGCCA ACATGGACCC TGCCATGATG
    TTCCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT
    GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT
    CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC
    TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA
    GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA
    AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC
    GAGCAGGTGT CAGCAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45799
    Clone ID Related Accession (Same CDS sequence) XM_006532563.4 , XM_006532563.3 , XM_030245697.1
    Accession Version XM_006532563.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532563.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA
    GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC
    ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT
    GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA
    GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG
    GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT
    CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG
    CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC
    ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT
    GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA
    GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG
    CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC
    TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG
    ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT
    GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG
    GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG
    GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA
    CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC
    AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG
    GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA
    GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT
    GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT
    GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC
    AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA
    CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC
    GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC
    CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT
    ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT
    CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT
    ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT
    GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA
    CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA
    AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG
    GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG
    AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA
    TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG
    ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT
    ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG
    TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA
    AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT
    AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT
    CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA
    GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT
    ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG
    TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG
    GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT
    GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA
    GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA
    AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG
    TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT
    CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG
    CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA
    CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT
    CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT
    GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC
    TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG
    CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT
    GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT
    CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG
    GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA
    AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA
    GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC
    CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA
    CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA
    CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT
    GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT
    GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC
    CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA
    TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC
    TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA
    GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532626.1
    CDS456..5015
    Translation

    Target ORF information:

    RefSeq Version XM_006532563.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532563.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA
    GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC
    ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT
    GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA
    GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG
    GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT
    CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG
    CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC
    ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT
    GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA
    GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG
    CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC
    TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG
    ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT
    GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG
    GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG
    GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA
    CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC
    AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG
    GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA
    GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT
    GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT
    GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC
    AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA
    CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC
    GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC
    CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT
    ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT
    CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT
    ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT
    GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA
    CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA
    AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG
    GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG
    AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA
    TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG
    ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT
    ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG
    TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA
    AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT
    AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT
    CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA
    GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT
    ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG
    TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG
    GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT
    GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA
    GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA
    AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG
    TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT
    CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG
    CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA
    CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT
    CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT
    GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC
    TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG
    CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT
    GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT
    CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG
    GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA
    AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA
    GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC
    CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA
    CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA
    CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT
    GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT
    GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC
    CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA
    TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC
    TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA
    GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45799
    Clone ID Related Accession (Same CDS sequence) XM_006532563.4 , XM_006532563.3 , XM_030245697.1
    Accession Version XM_030245697.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA
    GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC
    ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT
    GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA
    GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG
    GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT
    CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG
    CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC
    ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT
    GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA
    GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG
    CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC
    TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG
    ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT
    GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG
    GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG
    GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA
    CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC
    AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG
    GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA
    GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT
    GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT
    GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC
    AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA
    CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC
    GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC
    CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT
    ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT
    CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT
    ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT
    GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA
    CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA
    AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG
    GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG
    AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA
    TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG
    ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT
    ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG
    TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA
    AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT
    AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT
    CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA
    GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT
    ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG
    TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG
    GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT
    GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA
    GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA
    AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG
    TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT
    CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG
    CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA
    CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT
    CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT
    GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC
    TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG
    CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT
    GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT
    CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG
    GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA
    AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA
    GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC
    CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA
    CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA
    CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT
    GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT
    GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC
    CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA
    TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC
    TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA
    GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101557.1
    CDS385..4944
    Translation

    Target ORF information:

    RefSeq Version XM_030245697.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245697.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA
    GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC
    ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT
    GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA
    GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG
    GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT
    CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG
    CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC
    ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT
    GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA
    GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG
    CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC
    TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG
    ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT
    GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG
    GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG
    GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA
    CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC
    AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG
    GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA
    GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT
    GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT
    GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC
    AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA
    CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC
    GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC
    CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT
    ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT
    CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT
    ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT
    GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA
    CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA
    AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG
    GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG
    AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA
    TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG
    ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT
    ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG
    TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA
    AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT
    AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT
    CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA
    GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT
    ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG
    TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG
    GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT
    GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA
    GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA
    AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG
    TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT
    CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG
    CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA
    CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT
    CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT
    GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC
    TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG
    CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT
    GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT
    CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG
    GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA
    AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA
    GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC
    CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA
    CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA
    CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT
    GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT
    GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC
    CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA
    TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC
    TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA
    GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45800
    Clone ID Related Accession (Same CDS sequence) XM_006532564.3 , XM_006532564.4
    Accession Version XM_006532564.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532564.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC
    AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC
    AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA
    CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG
    CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA
    TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG
    CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC
    CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA
    GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC
    TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC
    ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG
    TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC
    TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC
    CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT
    CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT
    GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC
    CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA
    CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC
    TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG
    CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG
    ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC
    AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT
    GAAAACGAGC AGGTGTCAGC AGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532627.1
    CDS206..4732
    Translation

    Target ORF information:

    RefSeq Version XM_006532564.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532564.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC
    AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC
    AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA
    CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG
    CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA
    TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG
    CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC
    CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA
    GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC
    TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC
    ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG
    TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC
    TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC
    CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT
    CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT
    GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC
    CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA
    CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC
    TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG
    CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG
    ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC
    AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT
    GAAAACGAGC AGGTGTCAGC AGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77780
    Clone ID Related Accession (Same CDS sequence) XM_017314348.1 , XM_017314348.2
    Accession Version XM_017314348.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGAAAACGAG
    CAGGTGTCAG CAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169837.1
    CDS252..4769
    Translation

    Target ORF information:

    RefSeq Version XM_017314348.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGAAAACGAG
    CAGGTGTCAG CAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77780
    Clone ID Related Accession (Same CDS sequence) XM_017314348.1 , XM_017314348.2
    Accession Version XM_017314348.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGAAAACGAG
    CAGGTGTCAG CAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169837.1
    CDS206..4723
    Translation

    Target ORF information:

    RefSeq Version XM_017314348.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314348.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGAAAACGAG
    CAGGTGTCAG CAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19298
    Clone ID Related Accession (Same CDS sequence) NM_175258.4
    Accession Version NM_175258.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-27
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK167055.1, AK143331.1, AK220459.1 and AW493254.1. On Nov 16, 2011 this sequence version replaced NM_175258.3. Transcript Variant: This variant (2) lacks an in-frame coding exon, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT
    GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA
    CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA
    GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC
    TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA
    TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG
    GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC
    CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT
    AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA
    TCTCCTCAGA ACTCCCCACG CAAAGGTTAT ACACTCACAC CATCAAGTAA ATGTGACAAC
    TTGTCTGACT CCAGCCACAG TGAAATCTCC TCTCGGTCCA GCATTGTGAG CAATGGTTCT
    GTTGACTCCA TGTCTGCAGC TGGGCAGGAT GAACGCTGTT CCTCCCACAG TCTGGCAGTT
    CCTGAGCCCA CAGGGGCATT GGAAAAGACA GATCACCCTT CCGGGATCTC AGACCACAGT
    CAGCTTGCTC ACGGGTGGAT GCTGTCAAAG CCGTGTCTTA TCAAGGGTGT AGCTGTCTCA
    TCTTCTCTGA GCAGCGAAGA GATGTCTCAT GAGCATGTTG TGCTGGAAGC AGCTGACAGT
    GGGCGGGGCA GTTGGACTTC CTGTTCCAGC AGCTCCCATG ACAACTTCCA AAGCCTTCAA
    AATCAAAAAA GCTGGGACTT CCTGAATTCT TACAGACACA TGCATTTAGA TGACCCTATT
    GCAGAAGTTG AGCCCACTGA CTGTGAGCCC TGTGCCTGTC CTAAAGGCTG CTCAAGAACT
    TGTGGCCAGT GCAAAGGCAG CCTAGAGACG AACCAGCTGA GGCAGAGTTG GGCCTCCTCC
    AGCTCACTCT CGGACACATG TGAACCAAAC TACGGGACAG TTAAGCGGAG GGTGTTGGAA
    AGTGCACCAG CAGAGGCACC TGATGGCTTG GAGCCCAGGG ATACCACTGA CCCTGTTTAT
    AAAACTGTCA CTTCCAGTAC AGACAAGGGC TTGATTGTGT ACTGTGTCAC CTCACCCAAG
    AAAGGTGATA GGTATAGGGA GCCACCGCCC ACTCCTCCAG GATATCTGGG AATTTCTTTA
    GCGGACCTAA AGGAAGGACC CCACCCGCAC CTAAAACCTC CAGACTATAG TGTGGCAGTA
    CAGAGATCAA AGATGATGCT TAACAGCCTG TCTAGACTGC CGCCAGCTCC TCCCAGTAGC
    CACACCTCGG CCTGGGTTCC CTCGAAGATT GGATCCCAGC CTCAAAGGCA TAGCCATCCC
    AAGCTAGCAG ATGTGGCTGA TGCAGATAGC GAAGCAGATG AAAACGAGCA GGTGTCAGCA
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_780467.2
    CDS206..5011
    Translation

    Target ORF information:

    RefSeq Version NM_175258.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_175258.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT
    GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA
    CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA
    GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC
    TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA
    TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG
    GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC
    CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT
    AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA
    TCTCCTCAGA ACTCCCCACG CAAAGGTTAT ACACTCACAC CATCAAGTAA ATGTGACAAC
    TTGTCTGACT CCAGCCACAG TGAAATCTCC TCTCGGTCCA GCATTGTGAG CAATGGTTCT
    GTTGACTCCA TGTCTGCAGC TGGGCAGGAT GAACGCTGTT CCTCCCACAG TCTGGCAGTT
    CCTGAGCCCA CAGGGGCATT GGAAAAGACA GATCACCCTT CCGGGATCTC AGACCACAGT
    CAGCTTGCTC ACGGGTGGAT GCTGTCAAAG CCGTGTCTTA TCAAGGGTGT AGCTGTCTCA
    TCTTCTCTGA GCAGCGAAGA GATGTCTCAT GAGCATGTTG TGCTGGAAGC AGCTGACAGT
    GGGCGGGGCA GTTGGACTTC CTGTTCCAGC AGCTCCCATG ACAACTTCCA AAGCCTTCAA
    AATCAAAAAA GCTGGGACTT CCTGAATTCT TACAGACACA TGCATTTAGA TGACCCTATT
    GCAGAAGTTG AGCCCACTGA CTGTGAGCCC TGTGCCTGTC CTAAAGGCTG CTCAAGAACT
    TGTGGCCAGT GCAAAGGCAG CCTAGAGACG AACCAGCTGA GGCAGAGTTG GGCCTCCTCC
    AGCTCACTCT CGGACACATG TGAACCAAAC TACGGGACAG TTAAGCGGAG GGTGTTGGAA
    AGTGCACCAG CAGAGGCACC TGATGGCTTG GAGCCCAGGG ATACCACTGA CCCTGTTTAT
    AAAACTGTCA CTTCCAGTAC AGACAAGGGC TTGATTGTGT ACTGTGTCAC CTCACCCAAG
    AAAGGTGATA GGTATAGGGA GCCACCGCCC ACTCCTCCAG GATATCTGGG AATTTCTTTA
    GCGGACCTAA AGGAAGGACC CCACCCGCAC CTAAAACCTC CAGACTATAG TGTGGCAGTA
    CAGAGATCAA AGATGATGCT TAACAGCCTG TCTAGACTGC CGCCAGCTCC TCCCAGTAGC
    CACACCTCGG CCTGGGTTCC CTCGAAGATT GGATCCCAGC CTCAAAGGCA TAGCCATCCC
    AAGCTAGCAG ATGTGGCTGA TGCAGATAGC GAAGCAGATG AAAACGAGCA GGTGTCAGCA
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19412
    Clone ID Related Accession (Same CDS sequence) NM_001252494.1
    Accession Version NM_001252494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-27
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK167055.1, BC141182.1, AK045540.1, BC145503.1, AK220459.1 and AW493254.1. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC141182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT
    GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT
    CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC
    TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA
    GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA
    AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC
    GAGCAGGTGT CAGCAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001239423.1
    CDS206..5026
    Translation

    Target ORF information:

    RefSeq Version NM_001252494.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT
    GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT
    CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC
    TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA
    GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA
    AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC
    GAGCAGGTGT CAGCAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19500
    Clone ID Related Accession (Same CDS sequence) NM_001252496.1
    Accession Version NM_001252496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-27
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK167055.1, BC145503.1, AK045540.1, AK220459.1 and AW493254.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC145503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT
    GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT
    GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG
    GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG
    CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG
    ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC
    TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC
    CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC
    TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC
    CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT
    GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT
    GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA
    GACAAGGGCT TGATTGAAAA CGAGCAGGTG TCAGCAGTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001239425.1
    CDS206..4747
    Translation

    Target ORF information:

    RefSeq Version NM_001252496.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT
    GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT
    GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG
    GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG
    CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG
    ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC
    TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC
    CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC
    TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC
    CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT
    GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT
    GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA
    GACAAGGGCT TGATTGAAAA CGAGCAGGTG TCAGCAGTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45796
    Clone ID Related Accession (Same CDS sequence) XM_006532560.4 , XM_006532560.3
    Accession Version XM_006532560.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT
    GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT
    GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG
    GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG
    CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG
    ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC
    TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC
    CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC
    TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC
    CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT
    GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT
    GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA
    GACAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AAGGTGATAG GTATAGGGAG
    CCACCGCCCA CTCCTCCAGG ATATCTGGGA ATTTCTTTAG CGGACCTAAA GGAAGGACCC
    CACCCGCACC TAAAACCTCC AGACTATAGT GTGGCAGTAC AGAGATCAAA GATGATGCTT
    AACAGCCTGT CTAGACTGCC GCCAGCTCCT CCCAGTAGCC ACACCTCGGC CTGGGTTCCC
    TCGAAGATTG GATCCCAGCC TCAAAGGCAT AGCCATCCCA AGCTAGCAGA TGTGGCTGAT
    GCAGATAGCG AAGCAGATGA AAACGAGCAG GTGTCAGCAG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532623.1
    CDS251..5095
    Translation

    Target ORF information:

    RefSeq Version XM_006532560.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532560.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT
    GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT
    GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG
    GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG
    CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG
    ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC
    TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC
    CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC
    TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC
    CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT
    GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT
    GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA
    GACAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AAGGTGATAG GTATAGGGAG
    CCACCGCCCA CTCCTCCAGG ATATCTGGGA ATTTCTTTAG CGGACCTAAA GGAAGGACCC
    CACCCGCACC TAAAACCTCC AGACTATAGT GTGGCAGTAC AGAGATCAAA GATGATGCTT
    AACAGCCTGT CTAGACTGCC GCCAGCTCCT CCCAGTAGCC ACACCTCGGC CTGGGTTCCC
    TCGAAGATTG GATCCCAGCC TCAAAGGCAT AGCCATCCCA AGCTAGCAGA TGTGGCTGAT
    GCAGATAGCG AAGCAGATGA AAACGAGCAG GTGTCAGCAG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45797
    Clone ID Related Accession (Same CDS sequence) XM_006532561.3 , XM_006532561.4
    Accession Version XM_006532561.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC
    AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC
    AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA
    CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG
    CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA
    TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG
    CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC
    CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA
    GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC
    TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC
    ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG
    TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC
    TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC
    CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT
    CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT
    GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC
    CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA
    CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC
    TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG
    CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG
    ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC
    AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT
    GTGTACTGTG TCACCTCACC CAAGAAAGGT GATAGGTATA GGGAGCCACC GCCCACTCCT
    CCAGGATATC TGGGAATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCC GCACCTAAAA
    CCTCCAGACT ATAGTGTGGC AGTACAGAGA TCAAAGATGA TGCTTAACAG CCTGTCTAGA
    CTGCCGCCAG CTCCTCCCAG TAGCCACACC TCGGCCTGGG TTCCCTCGAA GATTGGATCC
    CAGCCTCAAA GGCATAGCCA TCCCAAGCTA GCAGATGTGG CTGATGCAGA TAGCGAAGCA
    GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532624.1
    CDS250..5079
    Translation

    Target ORF information:

    RefSeq Version XM_006532561.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532561.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC
    AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC
    AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA
    CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG
    CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA
    TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG
    CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC
    CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA
    GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC
    TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC
    ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG
    TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC
    TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC
    CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT
    CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT
    GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC
    CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA
    CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC
    TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG
    CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG
    ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC
    AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT
    GTGTACTGTG TCACCTCACC CAAGAAAGGT GATAGGTATA GGGAGCCACC GCCCACTCCT
    CCAGGATATC TGGGAATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCC GCACCTAAAA
    CCTCCAGACT ATAGTGTGGC AGTACAGAGA TCAAAGATGA TGCTTAACAG CCTGTCTAGA
    CTGCCGCCAG CTCCTCCCAG TAGCCACACC TCGGCCTGGG TTCCCTCGAA GATTGGATCC
    CAGCCTCAAA GGCATAGCCA TCCCAAGCTA GCAGATGTGG CTGATGCAGA TAGCGAAGCA
    GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45798
    Clone ID Related Accession (Same CDS sequence) XM_006532562.3 , XM_006532562.2
    Accession Version XM_006532562.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTACAA GAGCACGCTA TGAGAGATAC AGTGGCAATC AAATGCTTTT TTGTTCAGAA 
    ACTATTGCCA GATGTTGGTA TATCCTGCTT TCTGGATCTG TACTTGTAAA AGATTCTATG
    GTCCTTCCTC CTTGCAGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT
    GTGTTAGAAC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGACAGTATT
    TTACAAAGAG AAATTCCTGC CAGACAGTCT CGACGAAGGT TTCGGAAAAT TAACTATAAA
    GGAGAACGCC AAACCATAAT AGATGGAGTG GATATTAATA ACTACCTTTC GCTTCCAGCT
    GATCTTACCA AGATGCACCT CACAGACAAC CCTCATCCAC AGGTGACGCA TGTGTCTTCT
    AGTCAGTCTG GCTGTAGCAT TGCCAGTGAC TCTGGGAGCA GCAGTTTATC CGATATCTAT
    CAGGCTACAG AGAGTGAAGT AGGAGATGTA GATTTAACAC GCCTTCCAGA AGGACCTGTT
    GATTCTGAGG ATGAAGAGGA GGAGGAAGAA GAGATTGATA GAACAGATCC ACTGCAGGGT
    CGAGATCTTG TTCGGGAGTG TCTTGAAAAG GAACCCGCAG ACAAAACTGA TGATGATGTT
    GAACAGTTAC TTGAGTTCAT GCACCAGCTG CCTGCCTTTG CGAACATGAC CATGTCTGTA
    AGGAGAGAAC TCTGCTCCGT CATGGTTTTT GAAGTAGTAG AGCAGGCTGG GGCTGTTATT
    CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTCATTT TAAATGGCAC TGTAGAAATT
    AGTCATCCAG ATGGGAAAAT AGAAAATCTC TTCATGGGAA ATAGTTTTGG GATTGTCCCC
    ACGCTGGATA AGCAGCACAT GCATGGAGCT GTCAGGACCA AAGTCGATGA TTGTCAGTTT
    GTCTGCATAG CTCAACAAGA CTATTGGAGA ATTTTAAATC ATGTGGAAAA AAATACTCAC
    AAAGTTGAGG AAGAGGGAGA GATTGTCATG GTACATGAGC ACCGTGAGCT AGACCGGAGC
    GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTG
    ATAGAAGAAC ATTCTATTGT GGACCCAACA TACATAGAAG ATTTCCTCTT AACTTACAGG
    ACATTTCTTG AAACTCCTTT GGATGTTGGG ATCAAGCTTT TGGAATGGTT TAAGATTGAC
    AACCTAAGGG ATAAGGTGAC ACGGATTGTA CTCTTATGGG TAAATAATCA TTTTAATGAT
    TTTGAAGGTG ATCCTGCTAT GACTCAATTT CTAGAAGAAT TTGAAAGAAA TTTGGAAGAT
    ACAAAAATGA ATGGTCACCT CAGGTTATTG AATATTGCCT GTGCTGCAAA GGCTAAGTGG
    AGGCAAGTTG TACTGCAGAA GGCGTCCCGT GAGTCCCCGC TGCACTTCTG CCTTACTGGG
    GGCAGTGAGA AGGGATTTGG AGTCTTTGTT GAAGAAGTAG AATCTGGTAG CAAAGCTGCT
    GACGCAGGAC TGAAAAGGGG TGATCAGGTA ATGGAAGTCA ATGGACAAAA CTTTGAGAAT
    ATTACACTTG CTAAAGCCCT TGAAATTTTG AGAAATAATA CTCATCTTGC ACTTACTGTG
    AAGACCAACA TTTTTGTGTT CAAAGAATTG CTCAGTAGGA CTGAACAAGA GAAATCTGGT
    GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT TCAAGATGTC
    CCAGGGGATA TGGAGCAGGC ACCACAGGAG AAAGGAAATA AGAAAATTAA AGCAAATACC
    GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG
    CCTCCAAAAC TATTTAGTGA CGGAGGCCTG AGTCAATCAC AAGATGACAG CATTGTGGGA
    ACCAGGCACT GTAGGCACAG CCTGGCTATA ATGCCAATTC CCGGAACACT TTCATCCAGC
    AGCCCAGATC TCTTACAGCC TACCACTAGC ATGTTGGACT TTTCCAATCC TTCAGCTGTT
    GGTTTTTACT ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC
    TACATTATCA TCAGTAAGGA TACCACAGCT AAAGAAGTGG TCTGCCAAGC TGTACAGGAA
    TTCGGTTTGA CTGGTGCATC TGACACATAC TCCCTCTGTG AAGTGTCTGT TACTCCTGAG
    GGTGTTATAA AGCAGAGAAG ACTTCCGGAC CAGTTCTCCA AATTAGCTGA TAGAATTCAA
    CTCAATGGAA GGTATTACCT AAAAAACAAC ATGGAAACAG AAACATTGTG TTCTGATGAA
    GATGCTCAGG AACTACTTAA GGAAAGCCAG CTCTCCATGC TGCAGCTCAG TACCATCGAG
    GTCGCCACCC AGCTCTCCAT GAGAGACTTT GATTTATTTC GTAACATTGA GCCTACTGAA
    TACATTGATG ATCTTTTTAA ATTAGATTCC AAAACAGGAA ATACTCACTT GAAACAATTT
    GAGGATATTG TAAACCAAGA GACATTCTGG GTTGCCTCAG AGATTTTAAG TGAGTCAAAT
    CAGCTGAAAA GAATGAAGAT TATTAAACAT TTTATCAAAA TTGCTCTTCA TTGCCGAGAA
    TGCAAGAATT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ACCGGTAGCA
    CGACTCAGAG GCACTTGGGA AAAGTTGCCA AGCAAATATG AGAAACATCT TCAAGATCTA
    CAAGACCTTT TTGATCCATC TAGAAATATG GCAAAATACA GAAATATTCT TAGTAGTCAA
    AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTC
    CATGAAGGAA ATGACTCCAA AGTAGATGGC TTAGTAAACT TCGAGAAGCT AAGAATGATT
    GCCAAGGAAA TCCGTCATAT TATTCGAATG ACTTCTGCCA ACATGGACCC TGCCATGATG
    TTCCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT
    GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT
    CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC
    TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA
    GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA
    AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC
    GAGCAGGTGT CAGCAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532625.1
    CDS323..5023
    Translation

    Target ORF information:

    RefSeq Version XM_006532562.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532562.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTACAA GAGCACGCTA TGAGAGATAC AGTGGCAATC AAATGCTTTT TTGTTCAGAA 
    ACTATTGCCA GATGTTGGTA TATCCTGCTT TCTGGATCTG TACTTGTAAA AGATTCTATG
    GTCCTTCCTC CTTGCAGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT
    GTGTTAGAAC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGACAGTATT
    TTACAAAGAG AAATTCCTGC CAGACAGTCT CGACGAAGGT TTCGGAAAAT TAACTATAAA
    GGAGAACGCC AAACCATAAT AGATGGAGTG GATATTAATA ACTACCTTTC GCTTCCAGCT
    GATCTTACCA AGATGCACCT CACAGACAAC CCTCATCCAC AGGTGACGCA TGTGTCTTCT
    AGTCAGTCTG GCTGTAGCAT TGCCAGTGAC TCTGGGAGCA GCAGTTTATC CGATATCTAT
    CAGGCTACAG AGAGTGAAGT AGGAGATGTA GATTTAACAC GCCTTCCAGA AGGACCTGTT
    GATTCTGAGG ATGAAGAGGA GGAGGAAGAA GAGATTGATA GAACAGATCC ACTGCAGGGT
    CGAGATCTTG TTCGGGAGTG TCTTGAAAAG GAACCCGCAG ACAAAACTGA TGATGATGTT
    GAACAGTTAC TTGAGTTCAT GCACCAGCTG CCTGCCTTTG CGAACATGAC CATGTCTGTA
    AGGAGAGAAC TCTGCTCCGT CATGGTTTTT GAAGTAGTAG AGCAGGCTGG GGCTGTTATT
    CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTCATTT TAAATGGCAC TGTAGAAATT
    AGTCATCCAG ATGGGAAAAT AGAAAATCTC TTCATGGGAA ATAGTTTTGG GATTGTCCCC
    ACGCTGGATA AGCAGCACAT GCATGGAGCT GTCAGGACCA AAGTCGATGA TTGTCAGTTT
    GTCTGCATAG CTCAACAAGA CTATTGGAGA ATTTTAAATC ATGTGGAAAA AAATACTCAC
    AAAGTTGAGG AAGAGGGAGA GATTGTCATG GTACATGAGC ACCGTGAGCT AGACCGGAGC
    GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTG
    ATAGAAGAAC ATTCTATTGT GGACCCAACA TACATAGAAG ATTTCCTCTT AACTTACAGG
    ACATTTCTTG AAACTCCTTT GGATGTTGGG ATCAAGCTTT TGGAATGGTT TAAGATTGAC
    AACCTAAGGG ATAAGGTGAC ACGGATTGTA CTCTTATGGG TAAATAATCA TTTTAATGAT
    TTTGAAGGTG ATCCTGCTAT GACTCAATTT CTAGAAGAAT TTGAAAGAAA TTTGGAAGAT
    ACAAAAATGA ATGGTCACCT CAGGTTATTG AATATTGCCT GTGCTGCAAA GGCTAAGTGG
    AGGCAAGTTG TACTGCAGAA GGCGTCCCGT GAGTCCCCGC TGCACTTCTG CCTTACTGGG
    GGCAGTGAGA AGGGATTTGG AGTCTTTGTT GAAGAAGTAG AATCTGGTAG CAAAGCTGCT
    GACGCAGGAC TGAAAAGGGG TGATCAGGTA ATGGAAGTCA ATGGACAAAA CTTTGAGAAT
    ATTACACTTG CTAAAGCCCT TGAAATTTTG AGAAATAATA CTCATCTTGC ACTTACTGTG
    AAGACCAACA TTTTTGTGTT CAAAGAATTG CTCAGTAGGA CTGAACAAGA GAAATCTGGT
    GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT TCAAGATGTC
    CCAGGGGATA TGGAGCAGGC ACCACAGGAG AAAGGAAATA AGAAAATTAA AGCAAATACC
    GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG
    CCTCCAAAAC TATTTAGTGA CGGAGGCCTG AGTCAATCAC AAGATGACAG CATTGTGGGA
    ACCAGGCACT GTAGGCACAG CCTGGCTATA ATGCCAATTC CCGGAACACT TTCATCCAGC
    AGCCCAGATC TCTTACAGCC TACCACTAGC ATGTTGGACT TTTCCAATCC TTCAGCTGTT
    GGTTTTTACT ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC
    TACATTATCA TCAGTAAGGA TACCACAGCT AAAGAAGTGG TCTGCCAAGC TGTACAGGAA
    TTCGGTTTGA CTGGTGCATC TGACACATAC TCCCTCTGTG AAGTGTCTGT TACTCCTGAG
    GGTGTTATAA AGCAGAGAAG ACTTCCGGAC CAGTTCTCCA AATTAGCTGA TAGAATTCAA
    CTCAATGGAA GGTATTACCT AAAAAACAAC ATGGAAACAG AAACATTGTG TTCTGATGAA
    GATGCTCAGG AACTACTTAA GGAAAGCCAG CTCTCCATGC TGCAGCTCAG TACCATCGAG
    GTCGCCACCC AGCTCTCCAT GAGAGACTTT GATTTATTTC GTAACATTGA GCCTACTGAA
    TACATTGATG ATCTTTTTAA ATTAGATTCC AAAACAGGAA ATACTCACTT GAAACAATTT
    GAGGATATTG TAAACCAAGA GACATTCTGG GTTGCCTCAG AGATTTTAAG TGAGTCAAAT
    CAGCTGAAAA GAATGAAGAT TATTAAACAT TTTATCAAAA TTGCTCTTCA TTGCCGAGAA
    TGCAAGAATT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ACCGGTAGCA
    CGACTCAGAG GCACTTGGGA AAAGTTGCCA AGCAAATATG AGAAACATCT TCAAGATCTA
    CAAGACCTTT TTGATCCATC TAGAAATATG GCAAAATACA GAAATATTCT TAGTAGTCAA
    AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTC
    CATGAAGGAA ATGACTCCAA AGTAGATGGC TTAGTAAACT TCGAGAAGCT AAGAATGATT
    GCCAAGGAAA TCCGTCATAT TATTCGAATG ACTTCTGCCA ACATGGACCC TGCCATGATG
    TTCCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAT TGAGTCAAGG CAGCACAAAT
    TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG
    CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT
    TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA
    CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG
    ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA
    CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA
    GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT
    GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC
    TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA
    AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT
    GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC
    CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG
    ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG
    GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG
    GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC
    TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT
    TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA
    GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG
    AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG
    CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC
    ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT
    GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT
    CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC
    TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA
    GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA
    AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC
    GAGCAGGTGT CAGCAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45799
    Clone ID Related Accession (Same CDS sequence) XM_006532563.4 , XM_006532563.3 , XM_030245697.1
    Accession Version XM_006532563.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA
    GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC
    ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT
    GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA
    GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG
    GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT
    CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG
    CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC
    ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT
    GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA
    GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG
    CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC
    TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG
    ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT
    GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG
    GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG
    GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA
    CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC
    AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG
    GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA
    GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT
    GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT
    GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC
    AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA
    CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC
    GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC
    CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT
    ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT
    CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT
    ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT
    GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA
    CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA
    AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG
    GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG
    AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA
    TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG
    ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT
    ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG
    TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA
    AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT
    AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT
    CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA
    GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT
    ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG
    TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG
    GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT
    GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA
    GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA
    AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG
    TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT
    CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG
    CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA
    CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT
    CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT
    GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC
    TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG
    CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT
    GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT
    CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG
    GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA
    AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA
    GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC
    CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA
    CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA
    CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT
    GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT
    GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC
    CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA
    TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC
    TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA
    GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532626.1
    CDS465..5024
    Translation

    Target ORF information:

    RefSeq Version XM_006532563.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532563.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA
    GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC
    ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT
    GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA
    GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG
    GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT
    CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG
    CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC
    ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT
    GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA
    GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG
    CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC
    TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG
    ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT
    GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG
    GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG
    GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA
    CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC
    AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG
    GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA
    GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT
    GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT
    GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC
    AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA
    CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC
    GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC
    CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT
    ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT
    CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT
    ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT
    GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA
    CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA
    AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG
    GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG
    AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA
    TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG
    ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT
    ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG
    TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA
    AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT
    AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT
    CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA
    GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT
    ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG
    TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG
    GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT
    GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA
    GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA
    AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG
    TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT
    CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG
    CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA
    CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT
    CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT
    GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC
    TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG
    CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT
    GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT
    CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG
    GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA
    AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA
    GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC
    CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA
    CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA
    CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT
    GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT
    GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC
    CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA
    TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC
    TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA
    GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45800
    Clone ID Related Accession (Same CDS sequence) XM_006532564.3 , XM_006532564.4
    Accession Version XM_006532564.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC
    AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC
    AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA
    CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG
    CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA
    TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG
    CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC
    CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA
    GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC
    TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC
    ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG
    TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC
    TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC
    CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT
    CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT
    GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC
    CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA
    CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC
    TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG
    CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG
    ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC
    AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT
    GAAAACGAGC AGGTGTCAGC AGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532627.1
    CDS252..4778
    Translation

    Target ORF information:

    RefSeq Version XM_006532564.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532564.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC
    AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC
    AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA
    CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG
    CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA
    TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG
    CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC
    CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA
    GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC
    TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC
    ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG
    TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC
    TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC
    CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT
    CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT
    GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC
    CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA
    CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC
    TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG
    CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG
    ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC
    AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT
    GAAAACGAGC AGGTGTCAGC AGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45801
    Clone ID Related Accession (Same CDS sequence) XM_006532565.3 , XM_006532565.4
    Accession Version XM_006532565.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532565.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGCT
    GTTGGTTTTT ACTATATTCC TGATCAAGTT ATAAGAGTTT TCAAAGCAGA TCAACAAAGT
    TGCTACATTA TCATCAGTAA GGATACCACA GCTAAAGAAG TGGTCTGCCA AGCTGTACAG
    GAATTCGGTT TGACTGGTGC ATCTGACACA TACTCCCTCT GTGAAGTGTC TGTTACTCCT
    GAGGGTGTTA TAAAGCAGAG AAGACTTCCG GACCAGTTCT CCAAATTAGC TGATAGAATT
    CAACTCAATG GAAGGTATTA CCTAAAAAAC AACATGGAAA CAGAAACATT GTGTTCTGAT
    GAAGATGCTC AGGAACTACT TAAGGAAAGC CAGCTCTCCA TGCTGCAGCT CAGTACCATC
    GAGGTCGCCA CCCAGCTCTC CATGAGAGAC TTTGATTTAT TTCGTAACAT TGAGCCTACT
    GAATACATTG ATGATCTTTT TAAATTAGAT TCCAAAACAG GAAATACTCA CTTGAAACAA
    TTTGAGGATA TTGTAAACCA AGAGACATTC TGGGTTGCCT CAGAGATTTT AAGTGAGTCA
    AATCAGCTGA AAAGAATGAA GATTATTAAA CATTTTATCA AAATTGCTCT TCATTGCCGA
    GAATGCAAGA ATTTCAATTC CATGTTTGCA ATAATAAGTG GCTTGAACCT GGCACCGGTA
    GCACGACTCA GAGGCACTTG GGAAAAGTTG CCAAGCAAAT ATGAGAAACA TCTTCAAGAT
    CTACAAGACC TTTTTGATCC ATCTAGAAAT ATGGCAAAAT ACAGAAATAT TCTTAGTAGT
    CAAAGCATGC AGCCTCCAAT TATTCCACTC TTCCCTGTTG TCAAGAAAGA TATGACATTT
    CTCCATGAAG GAAATGACTC CAAAGTAGAT GGCTTAGTAA ACTTCGAGAA GCTAAGAATG
    ATTGCCAAGG AAATCCGTCA TATTATTCGA ATGACTTCTG CCAACATGGA CCCTGCCATG
    ATGTTCCGAC AGAGGAAGAA GAGGTGGCGG AGTCTGGGGT CATTGAGTCA AGGCAGCACA
    AATTCTAACA TGCTAGATGT CCAGGGAGGT GCTCACAAAA AAAGAGCACG CCGCAGCTCA
    CTGCTGAATG CCAAGAAACT GTATGAAGAT GCCCAAATGG CAAGGAAAGT GAAACAGTAT
    CTTTCTAGTC TGGACATAGA CACAGATGAG GAGAAGTTCC AGATGATGTC ACTGCAGTGG
    GAACCTGCAT ATGGTACCTT GACAAAGAAC TTAACTGAAA AAAGGTCAGC CAAATCATCT
    GAGATGTCTC CTGTACCATT GAGGTCAGTT GGCCAAACAG CTAAAGTCCA CTTGCATCAG
    CCACACAGAG TGAGCCAGGT GCTTCAGGTG CCAGCAGTTA ACTTGCACCC CATCCGGAAG
    AAAGGACAAG CCAAAGACCA TGTGCTGAGT ACAAGTTTAC CTCAGAAAGG TTTAGGACCA
    ACTGAGGAAG TGAGTGTTAA GAAACACACA GAAGACACTA TTTCTGTAGC GTCCTCTCTG
    CACTCTAGCC CTCCTGCATC TCCTCAGAAC TCCCCACGCA AAGGTTATAC ACTCACACCA
    TCAAGTAAAT GTGACAACTT GTCTGACTCC AGCCACAGTG AAATCTCCTC TCGGTCCAGC
    ATTGTGAGCA ATGGTTCTGT TGACTCCATG TCTGCAGCTG GGCAGGATGA ACGCTGTTCC
    TCCCACAGTC TGGCAGTTCC TGAGCCCACA GGGGCATTGG AAAAGACAGA TCACCCTTCC
    GGGATCTCAG ACCACAGTCA GCTTGCTCAC GGGTGGATGC TGTCAAAGCC GTGTCTTATC
    AAGGGTGTAG CTGTCTCATC TTCTCTGAGC AGCGAAGAGA TGTCTCATGA GCATGTTGTG
    CTGGAAGCAG CTGACAGTGG GCGGGGCAGT TGGACTTCCT GTTCCAGCAG CTCCCATGAC
    AACTTCCAAA GCCTTCAAAA TCAAAAAAGC TGGGACTTCC TGAATTCTTA CAGACACATG
    CATTTAGATG ACCCTATTGC AGAAGTTGAG CCCACTGACT GTGAGCCCTG TGCCTGTCCT
    AAAGGCTGCT CAAGAACTTG TGGCCAGTGC AAAGGCAGCC TAGAGACGAA CCAGCTGAGG
    CAGAGTTGGG CCTCCTCCAG CTCACTCTCG GACACATGTG AACCAAACTA CGGGACAGTT
    AAGCGGAGGG TGTTGGAAAG TGCACCAGCA GAGGCACCTG ATGGCTTGGA GCCCAGGGAT
    ACCACTGACC CTGTTTATAA AACTGTCACT TCCAGTACAG ACAAGGGCTT GATTGTGTAC
    TGTGTCACCT CACCCAAGAA AGGTGATAGG TATAGGGAGC CACCGCCCAC TCCTCCAGGA
    TATCTGGGAA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCCGCACCT AAAACCTCCA
    GACTATAGTG TGGCAGTACA GAGATCAAAG ATGATGCTTA ACAGCCTGTC TAGACTGCCG
    CCAGCTCCTC CCAGTAGCCA CACCTCGGCC TGGGTTCCCT CGAAGATTGG ATCCCAGCCT
    CAAAGGCATA GCCATCCCAA GCTAGCAGAT GTGGCTGATG CAGATAGCGA AGCAGATGAA
    AACGAGCAGG TGTCAGCAGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532628.1
    CDS1467..5870
    Translation

    Target ORF information:

    RefSeq Version XM_006532565.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532565.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGCT
    GTTGGTTTTT ACTATATTCC TGATCAAGTT ATAAGAGTTT TCAAAGCAGA TCAACAAAGT
    TGCTACATTA TCATCAGTAA GGATACCACA GCTAAAGAAG TGGTCTGCCA AGCTGTACAG
    GAATTCGGTT TGACTGGTGC ATCTGACACA TACTCCCTCT GTGAAGTGTC TGTTACTCCT
    GAGGGTGTTA TAAAGCAGAG AAGACTTCCG GACCAGTTCT CCAAATTAGC TGATAGAATT
    CAACTCAATG GAAGGTATTA CCTAAAAAAC AACATGGAAA CAGAAACATT GTGTTCTGAT
    GAAGATGCTC AGGAACTACT TAAGGAAAGC CAGCTCTCCA TGCTGCAGCT CAGTACCATC
    GAGGTCGCCA CCCAGCTCTC CATGAGAGAC TTTGATTTAT TTCGTAACAT TGAGCCTACT
    GAATACATTG ATGATCTTTT TAAATTAGAT TCCAAAACAG GAAATACTCA CTTGAAACAA
    TTTGAGGATA TTGTAAACCA AGAGACATTC TGGGTTGCCT CAGAGATTTT AAGTGAGTCA
    AATCAGCTGA AAAGAATGAA GATTATTAAA CATTTTATCA AAATTGCTCT TCATTGCCGA
    GAATGCAAGA ATTTCAATTC CATGTTTGCA ATAATAAGTG GCTTGAACCT GGCACCGGTA
    GCACGACTCA GAGGCACTTG GGAAAAGTTG CCAAGCAAAT ATGAGAAACA TCTTCAAGAT
    CTACAAGACC TTTTTGATCC ATCTAGAAAT ATGGCAAAAT ACAGAAATAT TCTTAGTAGT
    CAAAGCATGC AGCCTCCAAT TATTCCACTC TTCCCTGTTG TCAAGAAAGA TATGACATTT
    CTCCATGAAG GAAATGACTC CAAAGTAGAT GGCTTAGTAA ACTTCGAGAA GCTAAGAATG
    ATTGCCAAGG AAATCCGTCA TATTATTCGA ATGACTTCTG CCAACATGGA CCCTGCCATG
    ATGTTCCGAC AGAGGAAGAA GAGGTGGCGG AGTCTGGGGT CATTGAGTCA AGGCAGCACA
    AATTCTAACA TGCTAGATGT CCAGGGAGGT GCTCACAAAA AAAGAGCACG CCGCAGCTCA
    CTGCTGAATG CCAAGAAACT GTATGAAGAT GCCCAAATGG CAAGGAAAGT GAAACAGTAT
    CTTTCTAGTC TGGACATAGA CACAGATGAG GAGAAGTTCC AGATGATGTC ACTGCAGTGG
    GAACCTGCAT ATGGTACCTT GACAAAGAAC TTAACTGAAA AAAGGTCAGC CAAATCATCT
    GAGATGTCTC CTGTACCATT GAGGTCAGTT GGCCAAACAG CTAAAGTCCA CTTGCATCAG
    CCACACAGAG TGAGCCAGGT GCTTCAGGTG CCAGCAGTTA ACTTGCACCC CATCCGGAAG
    AAAGGACAAG CCAAAGACCA TGTGCTGAGT ACAAGTTTAC CTCAGAAAGG TTTAGGACCA
    ACTGAGGAAG TGAGTGTTAA GAAACACACA GAAGACACTA TTTCTGTAGC GTCCTCTCTG
    CACTCTAGCC CTCCTGCATC TCCTCAGAAC TCCCCACGCA AAGGTTATAC ACTCACACCA
    TCAAGTAAAT GTGACAACTT GTCTGACTCC AGCCACAGTG AAATCTCCTC TCGGTCCAGC
    ATTGTGAGCA ATGGTTCTGT TGACTCCATG TCTGCAGCTG GGCAGGATGA ACGCTGTTCC
    TCCCACAGTC TGGCAGTTCC TGAGCCCACA GGGGCATTGG AAAAGACAGA TCACCCTTCC
    GGGATCTCAG ACCACAGTCA GCTTGCTCAC GGGTGGATGC TGTCAAAGCC GTGTCTTATC
    AAGGGTGTAG CTGTCTCATC TTCTCTGAGC AGCGAAGAGA TGTCTCATGA GCATGTTGTG
    CTGGAAGCAG CTGACAGTGG GCGGGGCAGT TGGACTTCCT GTTCCAGCAG CTCCCATGAC
    AACTTCCAAA GCCTTCAAAA TCAAAAAAGC TGGGACTTCC TGAATTCTTA CAGACACATG
    CATTTAGATG ACCCTATTGC AGAAGTTGAG CCCACTGACT GTGAGCCCTG TGCCTGTCCT
    AAAGGCTGCT CAAGAACTTG TGGCCAGTGC AAAGGCAGCC TAGAGACGAA CCAGCTGAGG
    CAGAGTTGGG CCTCCTCCAG CTCACTCTCG GACACATGTG AACCAAACTA CGGGACAGTT
    AAGCGGAGGG TGTTGGAAAG TGCACCAGCA GAGGCACCTG ATGGCTTGGA GCCCAGGGAT
    ACCACTGACC CTGTTTATAA AACTGTCACT TCCAGTACAG ACAAGGGCTT GATTGTGTAC
    TGTGTCACCT CACCCAAGAA AGGTGATAGG TATAGGGAGC CACCGCCCAC TCCTCCAGGA
    TATCTGGGAA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCCGCACCT AAAACCTCCA
    GACTATAGTG TGGCAGTACA GAGATCAAAG ATGATGCTTA ACAGCCTGTC TAGACTGCCG
    CCAGCTCCTC CCAGTAGCCA CACCTCGGCC TGGGTTCCCT CGAAGATTGG ATCCCAGCCT
    CAAAGGCATA GCCATCCCAA GCTAGCAGAT GTGGCTGATG CAGATAGCGA AGCAGATGAA
    AACGAGCAGG TGTCAGCAGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45802
    Clone ID Related Accession (Same CDS sequence) XM_006532566.4 , XM_006532566.3
    Accession Version XM_006532566.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532566.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAAC AAAGTTGCTA CATTATCATC
    AGTAAGGATA CCACAGCTAA AGAAGTGGTC TGCCAAGCTG TACAGGAATT CGGTTTGACT
    GGTGCATCTG ACACATACTC CCTCTGTGAA GTGTCTGTTA CTCCTGAGGG TGTTATAAAG
    CAGAGAAGAC TTCCGGACCA GTTCTCCAAA TTAGCTGATA GAATTCAACT CAATGGAAGG
    TATTACCTAA AAAACAACAT GGAAACAGAA ACATTGTGTT CTGATGAAGA TGCTCAGGAA
    CTACTTAAGG AAAGCCAGCT CTCCATGCTG CAGCTCAGTA CCATCGAGGT CGCCACCCAG
    CTCTCCATGA GAGACTTTGA TTTATTTCGT AACATTGAGC CTACTGAATA CATTGATGAT
    CTTTTTAAAT TAGATTCCAA AACAGGAAAT ACTCACTTGA AACAATTTGA GGATATTGTA
    AACCAAGAGA CATTCTGGGT TGCCTCAGAG ATTTTAAGTG AGTCAAATCA GCTGAAAAGA
    ATGAAGATTA TTAAACATTT TATCAAAATT GCTCTTCATT GCCGAGAATG CAAGAATTTC
    AATTCCATGT TTGCAATAAT AAGTGGCTTG AACCTGGCAC CGGTAGCACG ACTCAGAGGC
    ACTTGGGAAA AGTTGCCAAG CAAATATGAG AAACATCTTC AAGATCTACA AGACCTTTTT
    GATCCATCTA GAAATATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTCCC TGTTGTCAAG AAAGATATGA CATTTCTCCA TGAAGGAAAT
    GACTCCAAAG TAGATGGCTT AGTAAACTTC GAGAAGCTAA GAATGATTGC CAAGGAAATC
    CGTCATATTA TTCGAATGAC TTCTGCCAAC ATGGACCCTG CCATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCATTG AGTCAAGGCA GCACAAATTC TAACATGCTA
    GATGTCCAGG GAGGTGCTCA CAAAAAAAGA GCACGCCGCA GCTCACTGCT GAATGCCAAG
    AAACTGTATG AAGATGCCCA AATGGCAAGG AAAGTGAAAC AGTATCTTTC TAGTCTGGAC
    ATAGACACAG ATGAGGAGAA GTTCCAGATG ATGTCACTGC AGTGGGAACC TGCATATGGT
    ACCTTGACAA AGAACTTAAC TGAAAAAAGG TCAGCCAAAT CATCTGAGAT GTCTCCTGTA
    CCATTGAGGT CAGTTGGCCA AACAGCTAAA GTCCACTTGC ATCAGCCACA CAGAGTGAGC
    CAGGTGCTTC AGGTGCCAGC AGTTAACTTG CACCCCATCC GGAAGAAAGG ACAAGCCAAA
    GACCATGTGC TGAGTACAAG TTTACCTCAG AAAGGTTTAG GACCAACTGA GGAAGTGAGT
    GTTAAGAAAC ACACAGAAGA CACTATTTCT GTAGCGTCCT CTCTGCACTC TAGCCCTCCT
    GCATCTCCTC AGAACTCCCC ACGCAAAGGT TATACACTCA CACCATCAAG TAAATGTGAC
    AACTTGTCTG ACTCCAGCCA CAGTGAAATC TCCTCTCGGT CCAGCATTGT GAGCAATGGT
    TCTGTTGACT CCATGTCTGC AGCTGGGCAG GATGAACGCT GTTCCTCCCA CAGTCTGGCA
    GTTCCTGAGC CCACAGGGGC ATTGGAAAAG ACAGATCACC CTTCCGGGAT CTCAGACCAC
    AGTCAGCTTG CTCACGGGTG GATGCTGTCA AAGCCGTGTC TTATCAAGGG TGTAGCTGTC
    TCATCTTCTC TGAGCAGCGA AGAGATGTCT CATGAGCATG TTGTGCTGGA AGCAGCTGAC
    AGTGGGCGGG GCAGTTGGAC TTCCTGTTCC AGCAGCTCCC ATGACAACTT CCAAAGCCTT
    CAAAATCAAA AAAGCTGGGA CTTCCTGAAT TCTTACAGAC ACATGCATTT AGATGACCCT
    ATTGCAGAAG TTGAGCCCAC TGACTGTGAG CCCTGTGCCT GTCCTAAAGG CTGCTCAAGA
    ACTTGTGGCC AGTGCAAAGG CAGCCTAGAG ACGAACCAGC TGAGGCAGAG TTGGGCCTCC
    TCCAGCTCAC TCTCGGACAC ATGTGAACCA AACTACGGGA CAGTTAAGCG GAGGGTGTTG
    GAAAGTGCAC CAGCAGAGGC ACCTGATGGC TTGGAGCCCA GGGATACCAC TGACCCTGTT
    TATAAAACTG TCACTTCCAG TACAGACAAG GGCTTGATTG TGTACTGTGT CACCTCACCC
    AAGAAAGGTG ATAGGTATAG GGAGCCACCG CCCACTCCTC CAGGATATCT GGGAATTTCT
    TTAGCGGACC TAAAGGAAGG ACCCCACCCG CACCTAAAAC CTCCAGACTA TAGTGTGGCA
    GTACAGAGAT CAAAGATGAT GCTTAACAGC CTGTCTAGAC TGCCGCCAGC TCCTCCCAGT
    AGCCACACCT CGGCCTGGGT TCCCTCGAAG ATTGGATCCC AGCCTCAAAG GCATAGCCAT
    CCCAAGCTAG CAGATGTGGC TGATGCAGAT AGCGAAGCAG ATGAAAACGA GCAGGTGTCA
    GCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532629.1
    CDS1466..5854
    Translation

    Target ORF information:

    RefSeq Version XM_006532566.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532566.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC
    TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT
    GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG
    GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT
    GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT
    ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA
    ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC
    CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG
    TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT
    CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG
    AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT
    TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC
    AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT
    GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA
    GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG
    TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT
    GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT
    GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG
    AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT
    GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT
    GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG
    GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC
    AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAAC AAAGTTGCTA CATTATCATC
    AGTAAGGATA CCACAGCTAA AGAAGTGGTC TGCCAAGCTG TACAGGAATT CGGTTTGACT
    GGTGCATCTG ACACATACTC CCTCTGTGAA GTGTCTGTTA CTCCTGAGGG TGTTATAAAG
    CAGAGAAGAC TTCCGGACCA GTTCTCCAAA TTAGCTGATA GAATTCAACT CAATGGAAGG
    TATTACCTAA AAAACAACAT GGAAACAGAA ACATTGTGTT CTGATGAAGA TGCTCAGGAA
    CTACTTAAGG AAAGCCAGCT CTCCATGCTG CAGCTCAGTA CCATCGAGGT CGCCACCCAG
    CTCTCCATGA GAGACTTTGA TTTATTTCGT AACATTGAGC CTACTGAATA CATTGATGAT
    CTTTTTAAAT TAGATTCCAA AACAGGAAAT ACTCACTTGA AACAATTTGA GGATATTGTA
    AACCAAGAGA CATTCTGGGT TGCCTCAGAG ATTTTAAGTG AGTCAAATCA GCTGAAAAGA
    ATGAAGATTA TTAAACATTT TATCAAAATT GCTCTTCATT GCCGAGAATG CAAGAATTTC
    AATTCCATGT TTGCAATAAT AAGTGGCTTG AACCTGGCAC CGGTAGCACG ACTCAGAGGC
    ACTTGGGAAA AGTTGCCAAG CAAATATGAG AAACATCTTC AAGATCTACA AGACCTTTTT
    GATCCATCTA GAAATATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTCCC TGTTGTCAAG AAAGATATGA CATTTCTCCA TGAAGGAAAT
    GACTCCAAAG TAGATGGCTT AGTAAACTTC GAGAAGCTAA GAATGATTGC CAAGGAAATC
    CGTCATATTA TTCGAATGAC TTCTGCCAAC ATGGACCCTG CCATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCATTG AGTCAAGGCA GCACAAATTC TAACATGCTA
    GATGTCCAGG GAGGTGCTCA CAAAAAAAGA GCACGCCGCA GCTCACTGCT GAATGCCAAG
    AAACTGTATG AAGATGCCCA AATGGCAAGG AAAGTGAAAC AGTATCTTTC TAGTCTGGAC
    ATAGACACAG ATGAGGAGAA GTTCCAGATG ATGTCACTGC AGTGGGAACC TGCATATGGT
    ACCTTGACAA AGAACTTAAC TGAAAAAAGG TCAGCCAAAT CATCTGAGAT GTCTCCTGTA
    CCATTGAGGT CAGTTGGCCA AACAGCTAAA GTCCACTTGC ATCAGCCACA CAGAGTGAGC
    CAGGTGCTTC AGGTGCCAGC AGTTAACTTG CACCCCATCC GGAAGAAAGG ACAAGCCAAA
    GACCATGTGC TGAGTACAAG TTTACCTCAG AAAGGTTTAG GACCAACTGA GGAAGTGAGT
    GTTAAGAAAC ACACAGAAGA CACTATTTCT GTAGCGTCCT CTCTGCACTC TAGCCCTCCT
    GCATCTCCTC AGAACTCCCC ACGCAAAGGT TATACACTCA CACCATCAAG TAAATGTGAC
    AACTTGTCTG ACTCCAGCCA CAGTGAAATC TCCTCTCGGT CCAGCATTGT GAGCAATGGT
    TCTGTTGACT CCATGTCTGC AGCTGGGCAG GATGAACGCT GTTCCTCCCA CAGTCTGGCA
    GTTCCTGAGC CCACAGGGGC ATTGGAAAAG ACAGATCACC CTTCCGGGAT CTCAGACCAC
    AGTCAGCTTG CTCACGGGTG GATGCTGTCA AAGCCGTGTC TTATCAAGGG TGTAGCTGTC
    TCATCTTCTC TGAGCAGCGA AGAGATGTCT CATGAGCATG TTGTGCTGGA AGCAGCTGAC
    AGTGGGCGGG GCAGTTGGAC TTCCTGTTCC AGCAGCTCCC ATGACAACTT CCAAAGCCTT
    CAAAATCAAA AAAGCTGGGA CTTCCTGAAT TCTTACAGAC ACATGCATTT AGATGACCCT
    ATTGCAGAAG TTGAGCCCAC TGACTGTGAG CCCTGTGCCT GTCCTAAAGG CTGCTCAAGA
    ACTTGTGGCC AGTGCAAAGG CAGCCTAGAG ACGAACCAGC TGAGGCAGAG TTGGGCCTCC
    TCCAGCTCAC TCTCGGACAC ATGTGAACCA AACTACGGGA CAGTTAAGCG GAGGGTGTTG
    GAAAGTGCAC CAGCAGAGGC ACCTGATGGC TTGGAGCCCA GGGATACCAC TGACCCTGTT
    TATAAAACTG TCACTTCCAG TACAGACAAG GGCTTGATTG TGTACTGTGT CACCTCACCC
    AAGAAAGGTG ATAGGTATAG GGAGCCACCG CCCACTCCTC CAGGATATCT GGGAATTTCT
    TTAGCGGACC TAAAGGAAGG ACCCCACCCG CACCTAAAAC CTCCAGACTA TAGTGTGGCA
    GTACAGAGAT CAAAGATGAT GCTTAACAGC CTGTCTAGAC TGCCGCCAGC TCCTCCCAGT
    AGCCACACCT CGGCCTGGGT TCCCTCGAAG ATTGGATCCC AGCCTCAAAG GCATAGCCAT
    CCCAAGCTAG CAGATGTGGC TGATGCAGAT AGCGAAGCAG ATGAAAACGA GCAGGTGTCA
    GCAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45803
    Clone ID Related Accession (Same CDS sequence) XM_006532567.4 , XM_006532567.3
    Accession Version XM_006532567.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532567.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGTTGGCA GTATCCTATC TGATCACAGC ATCCTGTTGC GTCTCTCAGG GTCTTCCTCA
    TCCCCTCCCC GTGAGCCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG
    GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACTGGGAGCA CAGCCAAAAG AACAGACAGG
    ACCAAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGGT GTGAGGACAC TACCAGGAGA
    AGGATGACAT CCCCGTTTAG AAGATTTAGG GAGAGAACGC TTTCAAGGGA GAGACTAGTC
    AACAACCAAA AAGAGGATGC ACATCACAAT CAAGCTACAG ACAGCTGTGA GAAAGTGAAG
    GATGTTGGAA GCAACATCAC AGATGAGAGA GGGAATGCCA TCTACGACTC GAGTAGCCAA
    GGCCACAGTA CCGCGCTCAG CTGCTTCTGT ACGCGGTTCA AGACTAAAAG GAGAAAGACT
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532630.1
    CDS4529..8794
    Translation

    Target ORF information:

    RefSeq Version XM_006532567.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532567.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA
    GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC
    TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC
    TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA
    ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC
    ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA
    TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA
    GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC
    TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC
    AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT
    GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA
    TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT
    GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA
    AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT
    GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA
    AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG
    GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC
    CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA
    AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA
    GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT
    AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA
    CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT
    ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC
    AAAGTTGGCA GTATCCTATC TGATCACAGC ATCCTGTTGC GTCTCTCAGG GTCTTCCTCA
    TCCCCTCCCC GTGAGCCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG
    GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACTGGGAGCA CAGCCAAAAG AACAGACAGG
    ACCAAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGGT GTGAGGACAC TACCAGGAGA
    AGGATGACAT CCCCGTTTAG AAGATTTAGG GAGAGAACGC TTTCAAGGGA GAGACTAGTC
    AACAACCAAA AAGAGGATGC ACATCACAAT CAAGCTACAG ACAGCTGTGA GAAAGTGAAG
    GATGTTGGAA GCAACATCAC AGATGAGAGA GGGAATGCCA TCTACGACTC GAGTAGCCAA
    GGCCACAGTA CCGCGCTCAG CTGCTTCTGT ACGCGGTTCA AGACTAAAAG GAGAAAGACT
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45804
    Clone ID Related Accession (Same CDS sequence) XM_006532568.4 , XM_006532568.3
    Accession Version XM_006532568.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 6 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532568.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT
    GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA
    CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA
    GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC
    TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA
    TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG
    GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC
    CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT
    AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA
    TCTCCTCAGA ACTCCCCACG CAAAGTTGGC AGTATCCTAT CTGATCACAG CATCCTGTTG
    CGTCTCTCAG GGTCTTCCTC ATCCCCTCCC CGTGAGCCCA GCACCAAGAT CAGTGGCCAG
    AGCTGCCCCG GAATAGGTGG GGTTTACTTG CAAAAGAAAA TCCTTCAGAT TACTGGGAGC
    ACAGCCAAAA GAACAGACAG GACCAAGAAG GCCACTGAGG AGAATAGAGA CAGGACAAGG
    TGTGAGGACA CTACCAGGAG AAGGATGACA TCCCCGTTTA GAAGATTTAG GGAGAGAACG
    CTTTCAAGGG AGAGACTAGT CAACAACCAA AAAGAGGATG CACATCACAA TCAAGCTACA
    GACAGCTGTG AGAAAGTGAA GGATGTTGGA AGCAACATCA CAGATGAGAG AGGGAATGCC
    ATCTACGACT CGAGTAGCCA AGGCCACAGT ACCGCGCTCA GCTGCTTCTG TACGCGGTTC
    AAGACTAAAA GGAGAAAGAC TCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532631.1
    CDS4529..8755
    Translation

    Target ORF information:

    RefSeq Version XM_006532568.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532568.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
    ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC
    CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA
    AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT
    AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT
    CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG
    AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA
    ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT
    GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC
    GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC
    TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA
    GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC
    ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG
    GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG
    ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA
    AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT
    GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA
    CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA
    GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG
    CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA
    TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA
    GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT
    GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC
    CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA
    GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA
    GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT
    AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA
    AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA
    TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA
    ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG
    GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA
    CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT
    GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT
    ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT
    AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT
    TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT
    ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG
    CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG
    AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT
    GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT
    TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA
    CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT
    CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG
    ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA
    AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT
    GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT
    GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA
    CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA
    GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC
    TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA
    TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG
    GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC
    CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT
    AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA
    TCTCCTCAGA ACTCCCCACG CAAAGTTGGC AGTATCCTAT CTGATCACAG CATCCTGTTG
    CGTCTCTCAG GGTCTTCCTC ATCCCCTCCC CGTGAGCCCA GCACCAAGAT CAGTGGCCAG
    AGCTGCCCCG GAATAGGTGG GGTTTACTTG CAAAAGAAAA TCCTTCAGAT TACTGGGAGC
    ACAGCCAAAA GAACAGACAG GACCAAGAAG GCCACTGAGG AGAATAGAGA CAGGACAAGG
    TGTGAGGACA CTACCAGGAG AAGGATGACA TCCCCGTTTA GAAGATTTAG GGAGAGAACG
    CTTTCAAGGG AGAGACTAGT CAACAACCAA AAAGAGGATG CACATCACAA TCAAGCTACA
    GACAGCTGTG AGAAAGTGAA GGATGTTGGA AGCAACATCA CAGATGAGAG AGGGAATGCC
    ATCTACGACT CGAGTAGCCA AGGCCACAGT ACCGCGCTCA GCTGCTTCTG TACGCGGTTC
    AAGACTAAAA GGAGAAAGAC TCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.