Rapgef6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rapgef6
Entrez Gene ID	192786
Full Name	Rap guanine nucleotide exchange factor (GEF) 6
Synonyms	A530068K01,AI844632,BB085664,C030018K18Rik,PDZ-GEF2,Pdzgef2,RA-GEF-2,Ragef2,mKIAA4052
General protein information	Preferred Names Rap guanine nucleotide exchange factor (GEF) 6 Names rap guanine nucleotide exchange factor 6 PDZ domain containing guanine nucleotide exchange factor (GEF) 2 PDZ domain containing guanine nucleotide exchange factor(GEF)1 rap guanine nucleotide exchange factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532563.4	XP_006532626.1	rap guanine nucleotide exchange factor 6 isoform X4
XM_006532566.4	XP_006532629.1	rap guanine nucleotide exchange factor 6 isoform X8
XM_006532568.4	XP_006532631.1	rap guanine nucleotide exchange factor 6 isoform X11
XM_006532564.3	XP_006532627.1	rap guanine nucleotide exchange factor 6 isoform X5
XM_006532566.3	XP_006532629.1	rap guanine nucleotide exchange factor 6 isoform X8
XM_006532562.3	XP_006532625.1	rap guanine nucleotide exchange factor 6 isoform X3
XM_006532564.4	XP_006532627.1	rap guanine nucleotide exchange factor 6 isoform X5
XM_006532567.4	XP_006532630.1	rap guanine nucleotide exchange factor 6 isoform X9
XM_030245698.1	XP_030101558.1	rap guanine nucleotide exchange factor 6 isoform X10
XM_006532561.3	XP_006532624.1	rap guanine nucleotide exchange factor 6 isoform X2
XM_006532562.2	XP_006532625.1	rap guanine nucleotide exchange factor 6 isoform X3
XM_017314348.1	XP_017169837.1	rap guanine nucleotide exchange factor 6 isoform X6
NM_001252498.1	NP_001239427.1	rap guanine nucleotide exchange factor 6 isoform 5
XM_006532560.4	XP_006532623.1	rap guanine nucleotide exchange factor 6 isoform X1
XM_017314348.2	XP_017169837.1	rap guanine nucleotide exchange factor 6 isoform X6
XM_006532567.3	XP_006532630.1	rap guanine nucleotide exchange factor 6 isoform X9
XM_006532568.3	XP_006532631.1	rap guanine nucleotide exchange factor 6 isoform X10
NM_001252494.1	NP_001239423.1	rap guanine nucleotide exchange factor 6 isoform 1
XM_006532561.4	XP_006532624.1	rap guanine nucleotide exchange factor 6 isoform X2
XM_006532560.3	XP_006532623.1	rap guanine nucleotide exchange factor 6 isoform X1
XM_006532563.3	XP_006532626.1	rap guanine nucleotide exchange factor 6 isoform X4
XM_006532565.3	XP_006532628.1	rap guanine nucleotide exchange factor 6 isoform X7
NM_001252497.1	NP_001239426.1	rap guanine nucleotide exchange factor 6 isoform 4
NM_001252496.1	NP_001239425.1	rap guanine nucleotide exchange factor 6 isoform 3
NM_175258.4	NP_780467.2	rap guanine nucleotide exchange factor 6 isoform 2
XM_030245697.1	XP_030101557.1	rap guanine nucleotide exchange factor 6 isoform X4
XM_006532565.4	XP_006532628.1	rap guanine nucleotide exchange factor 6 isoform X7

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RAPGEF6	XP_003586323.1
Homo sapiens (human)	RAPGEF6	NP_001157858.1
Canis lupus familiaris (dog)	RAPGEF6	XP_003431670.1
Mus musculus (house mouse)	Rapgef6	NP_780467.2
Gallus gallus (chicken)	RAPGEF6	XP_004945062.1
Pan troglodytes (chimpanzee)	RAPGEF6	XP_003339150.1
	RAPGEF6	XP_001101236.2
Rattus norvegicus (Norway rat)	Rapgef6	NP_001100473.1
Danio rerio (zebrafish)	rapgef6	XP_002666140.3
Caenorhabditis elegans (roundworm)	pxf-1	NP_001023389.1

Gene Ontology
Bibliography

Related articles in PubMed

Misregulation of an Activity-Dependent Splicing Network as a Common Mechanism Underlying Autism Spectrum Disorders.
Quesnel-Valli?res M, Dargaei Z, Irimia M, Gonatopoulos-Pournatzis T, Ip JY, Wu M, Sterne-Weiler T, Nakagawa S, Woodin MA, Blencowe BJ, Cordes SP
Molecular cell64(6)1023-1034(2016 Dec)

Crucial Role of Rapgef2 and Rapgef6, a Family of Guanine Nucleotide Exchange Factors for Rap1 Small GTPase, in Formation of Apical Surface Adherens Junctions and Neural Progenitor Development in the Mouse Cerebral Cortex.
Maeta K, Edamatsu H, Nishihara K, Ikutomo J, Bilasy SE, Kataoka T
eNeuro3(3)(2016 May-Jun)

Deletion of Rapgef6, a candidate schizophrenia susceptibility gene, disrupts amygdala function in mice.
Levy RJ, Kvajo M, Li Y, Tsvetkov E, Dong W, Yoshikawa Y, Kataoka T, Bolshakov VY, Karayiorgou M, Gogos JA
Translational psychiatry5e577(2015 Jun)

Essential roles for the splicing regulator nSR100/SRRM4 during nervous system development.
Quesnel-Valli?res M, Irimia M, Cordes SP, Blencowe BJ
Genes & development29(7)746-59(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study show that double knockout of Rapgef6 in Rapgef2-cKO mice (Rapgef2/6-dKO) results in marked enlargement of the ectopic cortical mass. While Rapgef2-cKO affects late-born neurons only, Rapgef2/6-dKO affects both early-born and late-born neurons in the developing brain.
Title: Crucial Role of Rapgef2 and Rapgef6, a Family of Guanine Nucleotide Exchange Factors for Rap1 Small GTPase, in Formation of Apical Surface Adherens Junctions and Neural Progenitor Development in the Mouse Cerebral Cortex.

Rapgef6 deletion mice were impaired in hippocampal and amygdalar function and had reduced spine density and primary dendrite number in pyramidal neurons of the CA3 hippocampal region.
Title: Deletion of Rapgef6, a candidate schizophrenia susceptibility gene, disrupts amygdala function in mice.

Data indicate that the testes of RA-GEF-2(-)knockout male mice were significantly smaller than those of wild-type mice and were markedly atrophied as well as hypospermatogenic.
Title: Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis.

These findings proved a crucial role of the cross-talk between two Ras-family GTPases M-Ras and Rap1, mediated by RA-GEF-2, in adhesion signaling.
Title: The M-Ras-RA-GEF-2-Rap1 pathway mediates tumor necrosis factor-alpha dependent regulation of integrin activation in splenocytes.

The following Rapgef6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rapgef6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19445	NM_001252498.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu19612	NM_001252497.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu45801	XM_006532565.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu45802	XM_006532566.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu45803	XM_006532567.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu45804	XM_006532568.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu85671	XM_030245698.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu45796	XM_006532560.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu45797	XM_006532561.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu45798	XM_006532562.3 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu45799	XM_006532563.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu45799	XM_030245697.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu45800	XM_006532564.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77780	XM_017314348.2 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu19298	NM_175258.4 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu19412	NM_001252494.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu19500	NM_001252496.1 Latest version!	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu45802	XM_006532566.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu45800	XM_006532564.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu45798	XM_006532562.2	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu77780	XM_017314348.1	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu45803	XM_006532567.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu45804	XM_006532568.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu45797	XM_006532561.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu45796	XM_006532560.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu45799	XM_006532563.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu45801	XM_006532565.3	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rapgef6 ( NM_001252498.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239427.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu19445
Clone ID Related Accession (Same CDS sequence)	NM_001252498.1
Accession Version	NM_001252498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK049669.1, AK164343.1 and AL607091.12. Transcript Variant: This variant (5) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting isoform (5) is shorter and has distinct N-and C-termini, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK049669.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAAGTAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239427.1
CDS	905..2206
Translation	MAFLVRCYANCLQPWASKLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVSK

Target ORF information:

RefSeq Version	NM_001252498.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252498.1

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAAGTAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( NM_001252497.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239426.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu19612
Clone ID Related Accession (Same CDS sequence)	NM_001252497.1
Accession Version	NM_001252497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB652674.1, AK164343.1, AK045540.1 and BB465827.1. Transcript Variant: This variant (4) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting isoform (4) is shorter and has distinct N-and C-termini, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK164343.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGGT 
AATGTTCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239426.1
CDS	905..2719
Translation	MAFLVRCYANCLQPWASKLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSGNVPM

Target ORF information:

RefSeq Version	NM_001252497.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252497.1

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGGT 
AATGTTCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532565.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532628.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA.

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CloneID	OMu45801
Clone ID Related Accession (Same CDS sequence)	XM_006532565.3 , XM_006532565.4
Accession Version	XM_006532565.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532565.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGCT 
GTTGGTTTTT ACTATATTCC TGATCAAGTT ATAAGAGTTT TCAAAGCAGA TCAACAAAGT 
TGCTACATTA TCATCAGTAA GGATACCACA GCTAAAGAAG TGGTCTGCCA AGCTGTACAG 
GAATTCGGTT TGACTGGTGC ATCTGACACA TACTCCCTCT GTGAAGTGTC TGTTACTCCT 
GAGGGTGTTA TAAAGCAGAG AAGACTTCCG GACCAGTTCT CCAAATTAGC TGATAGAATT 
CAACTCAATG GAAGGTATTA CCTAAAAAAC AACATGGAAA CAGAAACATT GTGTTCTGAT 
GAAGATGCTC AGGAACTACT TAAGGAAAGC CAGCTCTCCA TGCTGCAGCT CAGTACCATC 
GAGGTCGCCA CCCAGCTCTC CATGAGAGAC TTTGATTTAT TTCGTAACAT TGAGCCTACT 
GAATACATTG ATGATCTTTT TAAATTAGAT TCCAAAACAG GAAATACTCA CTTGAAACAA 
TTTGAGGATA TTGTAAACCA AGAGACATTC TGGGTTGCCT CAGAGATTTT AAGTGAGTCA 
AATCAGCTGA AAAGAATGAA GATTATTAAA CATTTTATCA AAATTGCTCT TCATTGCCGA 
GAATGCAAGA ATTTCAATTC CATGTTTGCA ATAATAAGTG GCTTGAACCT GGCACCGGTA 
GCACGACTCA GAGGCACTTG GGAAAAGTTG CCAAGCAAAT ATGAGAAACA TCTTCAAGAT 
CTACAAGACC TTTTTGATCC ATCTAGAAAT ATGGCAAAAT ACAGAAATAT TCTTAGTAGT 
CAAAGCATGC AGCCTCCAAT TATTCCACTC TTCCCTGTTG TCAAGAAAGA TATGACATTT 
CTCCATGAAG GAAATGACTC CAAAGTAGAT GGCTTAGTAA ACTTCGAGAA GCTAAGAATG 
ATTGCCAAGG AAATCCGTCA TATTATTCGA ATGACTTCTG CCAACATGGA CCCTGCCATG 
ATGTTCCGAC AGAGGAAGAA GAGGTGGCGG AGTCTGGGGT CATTGAGTCA AGGCAGCACA 
AATTCTAACA TGCTAGATGT CCAGGGAGGT GCTCACAAAA AAAGAGCACG CCGCAGCTCA 
CTGCTGAATG CCAAGAAACT GTATGAAGAT GCCCAAATGG CAAGGAAAGT GAAACAGTAT 
CTTTCTAGTC TGGACATAGA CACAGATGAG GAGAAGTTCC AGATGATGTC ACTGCAGTGG 
GAACCTGCAT ATGGTACCTT GACAAAGAAC TTAACTGAAA AAAGGTCAGC CAAATCATCT 
GAGATGTCTC CTGTACCATT GAGGTCAGTT GGCCAAACAG CTAAAGTCCA CTTGCATCAG 
CCACACAGAG TGAGCCAGGT GCTTCAGGTG CCAGCAGTTA ACTTGCACCC CATCCGGAAG 
AAAGGACAAG CCAAAGACCA TGTGCTGAGT ACAAGTTTAC CTCAGAAAGG TTTAGGACCA 
ACTGAGGAAG TGAGTGTTAA GAAACACACA GAAGACACTA TTTCTGTAGC GTCCTCTCTG 
CACTCTAGCC CTCCTGCATC TCCTCAGAAC TCCCCACGCA AAGGTTATAC ACTCACACCA 
TCAAGTAAAT GTGACAACTT GTCTGACTCC AGCCACAGTG AAATCTCCTC TCGGTCCAGC 
ATTGTGAGCA ATGGTTCTGT TGACTCCATG TCTGCAGCTG GGCAGGATGA ACGCTGTTCC 
TCCCACAGTC TGGCAGTTCC TGAGCCCACA GGGGCATTGG AAAAGACAGA TCACCCTTCC 
GGGATCTCAG ACCACAGTCA GCTTGCTCAC GGGTGGATGC TGTCAAAGCC GTGTCTTATC 
AAGGGTGTAG CTGTCTCATC TTCTCTGAGC AGCGAAGAGA TGTCTCATGA GCATGTTGTG 
CTGGAAGCAG CTGACAGTGG GCGGGGCAGT TGGACTTCCT GTTCCAGCAG CTCCCATGAC 
AACTTCCAAA GCCTTCAAAA TCAAAAAAGC TGGGACTTCC TGAATTCTTA CAGACACATG 
CATTTAGATG ACCCTATTGC AGAAGTTGAG CCCACTGACT GTGAGCCCTG TGCCTGTCCT 
AAAGGCTGCT CAAGAACTTG TGGCCAGTGC AAAGGCAGCC TAGAGACGAA CCAGCTGAGG 
CAGAGTTGGG CCTCCTCCAG CTCACTCTCG GACACATGTG AACCAAACTA CGGGACAGTT 
AAGCGGAGGG TGTTGGAAAG TGCACCAGCA GAGGCACCTG ATGGCTTGGA GCCCAGGGAT 
ACCACTGACC CTGTTTATAA AACTGTCACT TCCAGTACAG ACAAGGGCTT GATTGTGTAC 
TGTGTCACCT CACCCAAGAA AGGTGATAGG TATAGGGAGC CACCGCCCAC TCCTCCAGGA 
TATCTGGGAA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCCGCACCT AAAACCTCCA 
GACTATAGTG TGGCAGTACA GAGATCAAAG ATGATGCTTA ACAGCCTGTC TAGACTGCCG 
CCAGCTCCTC CCAGTAGCCA CACCTCGGCC TGGGTTCCCT CGAAGATTGG ATCCCAGCCT 
CAAAGGCATA GCCATCCCAA GCTAGCAGAT GTGGCTGATG CAGATAGCGA AGCAGATGAA 
AACGAGCAGG TGTCAGCAGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532628.1
CDS	7028..11431
Translation	MAFLVRCYANCLQPWASKLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532565.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532565.4

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGCT 
GTTGGTTTTT ACTATATTCC TGATCAAGTT ATAAGAGTTT TCAAAGCAGA TCAACAAAGT 
TGCTACATTA TCATCAGTAA GGATACCACA GCTAAAGAAG TGGTCTGCCA AGCTGTACAG 
GAATTCGGTT TGACTGGTGC ATCTGACACA TACTCCCTCT GTGAAGTGTC TGTTACTCCT 
GAGGGTGTTA TAAAGCAGAG AAGACTTCCG GACCAGTTCT CCAAATTAGC TGATAGAATT 
CAACTCAATG GAAGGTATTA CCTAAAAAAC AACATGGAAA CAGAAACATT GTGTTCTGAT 
GAAGATGCTC AGGAACTACT TAAGGAAAGC CAGCTCTCCA TGCTGCAGCT CAGTACCATC 
GAGGTCGCCA CCCAGCTCTC CATGAGAGAC TTTGATTTAT TTCGTAACAT TGAGCCTACT 
GAATACATTG ATGATCTTTT TAAATTAGAT TCCAAAACAG GAAATACTCA CTTGAAACAA 
TTTGAGGATA TTGTAAACCA AGAGACATTC TGGGTTGCCT CAGAGATTTT AAGTGAGTCA 
AATCAGCTGA AAAGAATGAA GATTATTAAA CATTTTATCA AAATTGCTCT TCATTGCCGA 
GAATGCAAGA ATTTCAATTC CATGTTTGCA ATAATAAGTG GCTTGAACCT GGCACCGGTA 
GCACGACTCA GAGGCACTTG GGAAAAGTTG CCAAGCAAAT ATGAGAAACA TCTTCAAGAT 
CTACAAGACC TTTTTGATCC ATCTAGAAAT ATGGCAAAAT ACAGAAATAT TCTTAGTAGT 
CAAAGCATGC AGCCTCCAAT TATTCCACTC TTCCCTGTTG TCAAGAAAGA TATGACATTT 
CTCCATGAAG GAAATGACTC CAAAGTAGAT GGCTTAGTAA ACTTCGAGAA GCTAAGAATG 
ATTGCCAAGG AAATCCGTCA TATTATTCGA ATGACTTCTG CCAACATGGA CCCTGCCATG 
ATGTTCCGAC AGAGGAAGAA GAGGTGGCGG AGTCTGGGGT CATTGAGTCA AGGCAGCACA 
AATTCTAACA TGCTAGATGT CCAGGGAGGT GCTCACAAAA AAAGAGCACG CCGCAGCTCA 
CTGCTGAATG CCAAGAAACT GTATGAAGAT GCCCAAATGG CAAGGAAAGT GAAACAGTAT 
CTTTCTAGTC TGGACATAGA CACAGATGAG GAGAAGTTCC AGATGATGTC ACTGCAGTGG 
GAACCTGCAT ATGGTACCTT GACAAAGAAC TTAACTGAAA AAAGGTCAGC CAAATCATCT 
GAGATGTCTC CTGTACCATT GAGGTCAGTT GGCCAAACAG CTAAAGTCCA CTTGCATCAG 
CCACACAGAG TGAGCCAGGT GCTTCAGGTG CCAGCAGTTA ACTTGCACCC CATCCGGAAG 
AAAGGACAAG CCAAAGACCA TGTGCTGAGT ACAAGTTTAC CTCAGAAAGG TTTAGGACCA 
ACTGAGGAAG TGAGTGTTAA GAAACACACA GAAGACACTA TTTCTGTAGC GTCCTCTCTG 
CACTCTAGCC CTCCTGCATC TCCTCAGAAC TCCCCACGCA AAGGTTATAC ACTCACACCA 
TCAAGTAAAT GTGACAACTT GTCTGACTCC AGCCACAGTG AAATCTCCTC TCGGTCCAGC 
ATTGTGAGCA ATGGTTCTGT TGACTCCATG TCTGCAGCTG GGCAGGATGA ACGCTGTTCC 
TCCCACAGTC TGGCAGTTCC TGAGCCCACA GGGGCATTGG AAAAGACAGA TCACCCTTCC 
GGGATCTCAG ACCACAGTCA GCTTGCTCAC GGGTGGATGC TGTCAAAGCC GTGTCTTATC 
AAGGGTGTAG CTGTCTCATC TTCTCTGAGC AGCGAAGAGA TGTCTCATGA GCATGTTGTG 
CTGGAAGCAG CTGACAGTGG GCGGGGCAGT TGGACTTCCT GTTCCAGCAG CTCCCATGAC 
AACTTCCAAA GCCTTCAAAA TCAAAAAAGC TGGGACTTCC TGAATTCTTA CAGACACATG 
CATTTAGATG ACCCTATTGC AGAAGTTGAG CCCACTGACT GTGAGCCCTG TGCCTGTCCT 
AAAGGCTGCT CAAGAACTTG TGGCCAGTGC AAAGGCAGCC TAGAGACGAA CCAGCTGAGG 
CAGAGTTGGG CCTCCTCCAG CTCACTCTCG GACACATGTG AACCAAACTA CGGGACAGTT 
AAGCGGAGGG TGTTGGAAAG TGCACCAGCA GAGGCACCTG ATGGCTTGGA GCCCAGGGAT 
ACCACTGACC CTGTTTATAA AACTGTCACT TCCAGTACAG ACAAGGGCTT GATTGTGTAC 
TGTGTCACCT CACCCAAGAA AGGTGATAGG TATAGGGAGC CACCGCCCAC TCCTCCAGGA 
TATCTGGGAA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCCGCACCT AAAACCTCCA 
GACTATAGTG TGGCAGTACA GAGATCAAAG ATGATGCTTA ACAGCCTGTC TAGACTGCCG 
CCAGCTCCTC CCAGTAGCCA CACCTCGGCC TGGGTTCCCT CGAAGATTGG ATCCCAGCCT 
CAAAGGCATA GCCATCCCAA GCTAGCAGAT GTGGCTGATG CAGATAGCGA AGCAGATGAA 
AACGAGCAGG TGTCAGCAGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532566.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532629.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu45802
Clone ID Related Accession (Same CDS sequence)	XM_006532566.4 , XM_006532566.3
Accession Version	XM_006532566.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532566.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAAC AAAGTTGCTA CATTATCATC 
AGTAAGGATA CCACAGCTAA AGAAGTGGTC TGCCAAGCTG TACAGGAATT CGGTTTGACT 
GGTGCATCTG ACACATACTC CCTCTGTGAA GTGTCTGTTA CTCCTGAGGG TGTTATAAAG 
CAGAGAAGAC TTCCGGACCA GTTCTCCAAA TTAGCTGATA GAATTCAACT CAATGGAAGG 
TATTACCTAA AAAACAACAT GGAAACAGAA ACATTGTGTT CTGATGAAGA TGCTCAGGAA 
CTACTTAAGG AAAGCCAGCT CTCCATGCTG CAGCTCAGTA CCATCGAGGT CGCCACCCAG 
CTCTCCATGA GAGACTTTGA TTTATTTCGT AACATTGAGC CTACTGAATA CATTGATGAT 
CTTTTTAAAT TAGATTCCAA AACAGGAAAT ACTCACTTGA AACAATTTGA GGATATTGTA 
AACCAAGAGA CATTCTGGGT TGCCTCAGAG ATTTTAAGTG AGTCAAATCA GCTGAAAAGA 
ATGAAGATTA TTAAACATTT TATCAAAATT GCTCTTCATT GCCGAGAATG CAAGAATTTC 
AATTCCATGT TTGCAATAAT AAGTGGCTTG AACCTGGCAC CGGTAGCACG ACTCAGAGGC 
ACTTGGGAAA AGTTGCCAAG CAAATATGAG AAACATCTTC AAGATCTACA AGACCTTTTT 
GATCCATCTA GAAATATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTCCC TGTTGTCAAG AAAGATATGA CATTTCTCCA TGAAGGAAAT 
GACTCCAAAG TAGATGGCTT AGTAAACTTC GAGAAGCTAA GAATGATTGC CAAGGAAATC 
CGTCATATTA TTCGAATGAC TTCTGCCAAC ATGGACCCTG CCATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCATTG AGTCAAGGCA GCACAAATTC TAACATGCTA 
GATGTCCAGG GAGGTGCTCA CAAAAAAAGA GCACGCCGCA GCTCACTGCT GAATGCCAAG 
AAACTGTATG AAGATGCCCA AATGGCAAGG AAAGTGAAAC AGTATCTTTC TAGTCTGGAC 
ATAGACACAG ATGAGGAGAA GTTCCAGATG ATGTCACTGC AGTGGGAACC TGCATATGGT 
ACCTTGACAA AGAACTTAAC TGAAAAAAGG TCAGCCAAAT CATCTGAGAT GTCTCCTGTA 
CCATTGAGGT CAGTTGGCCA AACAGCTAAA GTCCACTTGC ATCAGCCACA CAGAGTGAGC 
CAGGTGCTTC AGGTGCCAGC AGTTAACTTG CACCCCATCC GGAAGAAAGG ACAAGCCAAA 
GACCATGTGC TGAGTACAAG TTTACCTCAG AAAGGTTTAG GACCAACTGA GGAAGTGAGT 
GTTAAGAAAC ACACAGAAGA CACTATTTCT GTAGCGTCCT CTCTGCACTC TAGCCCTCCT 
GCATCTCCTC AGAACTCCCC ACGCAAAGGT TATACACTCA CACCATCAAG TAAATGTGAC 
AACTTGTCTG ACTCCAGCCA CAGTGAAATC TCCTCTCGGT CCAGCATTGT GAGCAATGGT 
TCTGTTGACT CCATGTCTGC AGCTGGGCAG GATGAACGCT GTTCCTCCCA CAGTCTGGCA 
GTTCCTGAGC CCACAGGGGC ATTGGAAAAG ACAGATCACC CTTCCGGGAT CTCAGACCAC 
AGTCAGCTTG CTCACGGGTG GATGCTGTCA AAGCCGTGTC TTATCAAGGG TGTAGCTGTC 
TCATCTTCTC TGAGCAGCGA AGAGATGTCT CATGAGCATG TTGTGCTGGA AGCAGCTGAC 
AGTGGGCGGG GCAGTTGGAC TTCCTGTTCC AGCAGCTCCC ATGACAACTT CCAAAGCCTT 
CAAAATCAAA AAAGCTGGGA CTTCCTGAAT TCTTACAGAC ACATGCATTT AGATGACCCT 
ATTGCAGAAG TTGAGCCCAC TGACTGTGAG CCCTGTGCCT GTCCTAAAGG CTGCTCAAGA 
ACTTGTGGCC AGTGCAAAGG CAGCCTAGAG ACGAACCAGC TGAGGCAGAG TTGGGCCTCC 
TCCAGCTCAC TCTCGGACAC ATGTGAACCA AACTACGGGA CAGTTAAGCG GAGGGTGTTG 
GAAAGTGCAC CAGCAGAGGC ACCTGATGGC TTGGAGCCCA GGGATACCAC TGACCCTGTT 
TATAAAACTG TCACTTCCAG TACAGACAAG GGCTTGATTG TGTACTGTGT CACCTCACCC 
AAGAAAGGTG ATAGGTATAG GGAGCCACCG CCCACTCCTC CAGGATATCT GGGAATTTCT 
TTAGCGGACC TAAAGGAAGG ACCCCACCCG CACCTAAAAC CTCCAGACTA TAGTGTGGCA 
GTACAGAGAT CAAAGATGAT GCTTAACAGC CTGTCTAGAC TGCCGCCAGC TCCTCCCAGT 
AGCCACACCT CGGCCTGGGT TCCCTCGAAG ATTGGATCCC AGCCTCAAAG GCATAGCCAT 
CCCAAGCTAG CAGATGTGGC TGATGCAGAT AGCGAAGCAG ATGAAAACGA GCAGGTGTCA 
GCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532629.1
CDS	947..5335
Translation	MAFLVRCYANCLQPWASKLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532566.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532566.4

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAAC AAAGTTGCTA CATTATCATC 
AGTAAGGATA CCACAGCTAA AGAAGTGGTC TGCCAAGCTG TACAGGAATT CGGTTTGACT 
GGTGCATCTG ACACATACTC CCTCTGTGAA GTGTCTGTTA CTCCTGAGGG TGTTATAAAG 
CAGAGAAGAC TTCCGGACCA GTTCTCCAAA TTAGCTGATA GAATTCAACT CAATGGAAGG 
TATTACCTAA AAAACAACAT GGAAACAGAA ACATTGTGTT CTGATGAAGA TGCTCAGGAA 
CTACTTAAGG AAAGCCAGCT CTCCATGCTG CAGCTCAGTA CCATCGAGGT CGCCACCCAG 
CTCTCCATGA GAGACTTTGA TTTATTTCGT AACATTGAGC CTACTGAATA CATTGATGAT 
CTTTTTAAAT TAGATTCCAA AACAGGAAAT ACTCACTTGA AACAATTTGA GGATATTGTA 
AACCAAGAGA CATTCTGGGT TGCCTCAGAG ATTTTAAGTG AGTCAAATCA GCTGAAAAGA 
ATGAAGATTA TTAAACATTT TATCAAAATT GCTCTTCATT GCCGAGAATG CAAGAATTTC 
AATTCCATGT TTGCAATAAT AAGTGGCTTG AACCTGGCAC CGGTAGCACG ACTCAGAGGC 
ACTTGGGAAA AGTTGCCAAG CAAATATGAG AAACATCTTC AAGATCTACA AGACCTTTTT 
GATCCATCTA GAAATATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTCCC TGTTGTCAAG AAAGATATGA CATTTCTCCA TGAAGGAAAT 
GACTCCAAAG TAGATGGCTT AGTAAACTTC GAGAAGCTAA GAATGATTGC CAAGGAAATC 
CGTCATATTA TTCGAATGAC TTCTGCCAAC ATGGACCCTG CCATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCATTG AGTCAAGGCA GCACAAATTC TAACATGCTA 
GATGTCCAGG GAGGTGCTCA CAAAAAAAGA GCACGCCGCA GCTCACTGCT GAATGCCAAG 
AAACTGTATG AAGATGCCCA AATGGCAAGG AAAGTGAAAC AGTATCTTTC TAGTCTGGAC 
ATAGACACAG ATGAGGAGAA GTTCCAGATG ATGTCACTGC AGTGGGAACC TGCATATGGT 
ACCTTGACAA AGAACTTAAC TGAAAAAAGG TCAGCCAAAT CATCTGAGAT GTCTCCTGTA 
CCATTGAGGT CAGTTGGCCA AACAGCTAAA GTCCACTTGC ATCAGCCACA CAGAGTGAGC 
CAGGTGCTTC AGGTGCCAGC AGTTAACTTG CACCCCATCC GGAAGAAAGG ACAAGCCAAA 
GACCATGTGC TGAGTACAAG TTTACCTCAG AAAGGTTTAG GACCAACTGA GGAAGTGAGT 
GTTAAGAAAC ACACAGAAGA CACTATTTCT GTAGCGTCCT CTCTGCACTC TAGCCCTCCT 
GCATCTCCTC AGAACTCCCC ACGCAAAGGT TATACACTCA CACCATCAAG TAAATGTGAC 
AACTTGTCTG ACTCCAGCCA CAGTGAAATC TCCTCTCGGT CCAGCATTGT GAGCAATGGT 
TCTGTTGACT CCATGTCTGC AGCTGGGCAG GATGAACGCT GTTCCTCCCA CAGTCTGGCA 
GTTCCTGAGC CCACAGGGGC ATTGGAAAAG ACAGATCACC CTTCCGGGAT CTCAGACCAC 
AGTCAGCTTG CTCACGGGTG GATGCTGTCA AAGCCGTGTC TTATCAAGGG TGTAGCTGTC 
TCATCTTCTC TGAGCAGCGA AGAGATGTCT CATGAGCATG TTGTGCTGGA AGCAGCTGAC 
AGTGGGCGGG GCAGTTGGAC TTCCTGTTCC AGCAGCTCCC ATGACAACTT CCAAAGCCTT 
CAAAATCAAA AAAGCTGGGA CTTCCTGAAT TCTTACAGAC ACATGCATTT AGATGACCCT 
ATTGCAGAAG TTGAGCCCAC TGACTGTGAG CCCTGTGCCT GTCCTAAAGG CTGCTCAAGA 
ACTTGTGGCC AGTGCAAAGG CAGCCTAGAG ACGAACCAGC TGAGGCAGAG TTGGGCCTCC 
TCCAGCTCAC TCTCGGACAC ATGTGAACCA AACTACGGGA CAGTTAAGCG GAGGGTGTTG 
GAAAGTGCAC CAGCAGAGGC ACCTGATGGC TTGGAGCCCA GGGATACCAC TGACCCTGTT 
TATAAAACTG TCACTTCCAG TACAGACAAG GGCTTGATTG TGTACTGTGT CACCTCACCC 
AAGAAAGGTG ATAGGTATAG GGAGCCACCG CCCACTCCTC CAGGATATCT GGGAATTTCT 
TTAGCGGACC TAAAGGAAGG ACCCCACCCG CACCTAAAAC CTCCAGACTA TAGTGTGGCA 
GTACAGAGAT CAAAGATGAT GCTTAACAGC CTGTCTAGAC TGCCGCCAGC TCCTCCCAGT 
AGCCACACCT CGGCCTGGGT TCCCTCGAAG ATTGGATCCC AGCCTCAAAG GCATAGCCAT 
CCCAAGCTAG CAGATGTGGC TGATGCAGAT AGCGAAGCAG ATGAAAACGA GCAGGTGTCA 
GCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532567.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532630.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA.

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CloneID	OMu45803
Clone ID Related Accession (Same CDS sequence)	XM_006532567.4 , XM_006532567.3
Accession Version	XM_006532567.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532567.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGTTGGCA GTATCCTATC TGATCACAGC ATCCTGTTGC GTCTCTCAGG GTCTTCCTCA 
TCCCCTCCCC GTGAGCCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG 
GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACTGGGAGCA CAGCCAAAAG AACAGACAGG 
ACCAAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGGT GTGAGGACAC TACCAGGAGA 
AGGATGACAT CCCCGTTTAG AAGATTTAGG GAGAGAACGC TTTCAAGGGA GAGACTAGTC 
AACAACCAAA AAGAGGATGC ACATCACAAT CAAGCTACAG ACAGCTGTGA GAAAGTGAAG 
GATGTTGGAA GCAACATCAC AGATGAGAGA GGGAATGCCA TCTACGACTC GAGTAGCCAA 
GGCCACAGTA CCGCGCTCAG CTGCTTCTGT ACGCGGTTCA AGACTAAAAG GAGAAAGACT 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532630.1
CDS	206..4471
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKVGSILSDHSILLRLSGSSSSPPREPSTKISGQSCPGIGGVYLQKKILQITGSTAKRTDRTKKATEENRDRTRCEDTTRRRMTSPFRRFRERTLSRERLVNNQKEDAHHNQATDSCEKVKDVGSNITDERGNAIYDSSSQGHSTALSCFCTRFKTKRRKTL

Target ORF information:

RefSeq Version	XM_006532567.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532567.4

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGTTGGCA GTATCCTATC TGATCACAGC ATCCTGTTGC GTCTCTCAGG GTCTTCCTCA 
TCCCCTCCCC GTGAGCCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG 
GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACTGGGAGCA CAGCCAAAAG AACAGACAGG 
ACCAAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGGT GTGAGGACAC TACCAGGAGA 
AGGATGACAT CCCCGTTTAG AAGATTTAGG GAGAGAACGC TTTCAAGGGA GAGACTAGTC 
AACAACCAAA AAGAGGATGC ACATCACAAT CAAGCTACAG ACAGCTGTGA GAAAGTGAAG 
GATGTTGGAA GCAACATCAC AGATGAGAGA GGGAATGCCA TCTACGACTC GAGTAGCCAA 
GGCCACAGTA CCGCGCTCAG CTGCTTCTGT ACGCGGTTCA AGACTAAAAG GAGAAAGACT 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532568.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532631.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X12, mRNA.

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CloneID	OMu45804
Clone ID Related Accession (Same CDS sequence)	XM_006532568.4 , XM_006532568.3
Accession Version	XM_006532568.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532568.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT 
GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA 
CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA 
GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC 
TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA 
TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG 
GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC 
CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT 
AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA 
TCTCCTCAGA ACTCCCCACG CAAAGTTGGC AGTATCCTAT CTGATCACAG CATCCTGTTG 
CGTCTCTCAG GGTCTTCCTC ATCCCCTCCC CGTGAGCCCA GCACCAAGAT CAGTGGCCAG 
AGCTGCCCCG GAATAGGTGG GGTTTACTTG CAAAAGAAAA TCCTTCAGAT TACTGGGAGC 
ACAGCCAAAA GAACAGACAG GACCAAGAAG GCCACTGAGG AGAATAGAGA CAGGACAAGG 
TGTGAGGACA CTACCAGGAG AAGGATGACA TCCCCGTTTA GAAGATTTAG GGAGAGAACG 
CTTTCAAGGG AGAGACTAGT CAACAACCAA AAAGAGGATG CACATCACAA TCAAGCTACA 
GACAGCTGTG AGAAAGTGAA GGATGTTGGA AGCAACATCA CAGATGAGAG AGGGAATGCC 
ATCTACGACT CGAGTAGCCA AGGCCACAGT ACCGCGCTCA GCTGCTTCTG TACGCGGTTC 
AAGACTAAAA GGAGAAAGAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532631.1
CDS	206..4432
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKVGSILSDHSILLRLSGSSSSPPREPSTKISGQSCPGIGGVYLQKKILQITGSTAKRTDRTKKATEENRDRTRCEDTTRRRMTSPFRRFRERTLSRERLVNNQKEDAHHNQATDSCEKVKDVGSNITDERGNAIYDSSSQGHSTALSCFCTRFKTKRRKTL

Target ORF information:

RefSeq Version	XM_006532568.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532568.4

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT 
GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA 
CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA 
GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC 
TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA 
TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG 
GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC 
CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT 
AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA 
TCTCCTCAGA ACTCCCCACG CAAAGTTGGC AGTATCCTAT CTGATCACAG CATCCTGTTG 
CGTCTCTCAG GGTCTTCCTC ATCCCCTCCC CGTGAGCCCA GCACCAAGAT CAGTGGCCAG 
AGCTGCCCCG GAATAGGTGG GGTTTACTTG CAAAAGAAAA TCCTTCAGAT TACTGGGAGC 
ACAGCCAAAA GAACAGACAG GACCAAGAAG GCCACTGAGG AGAATAGAGA CAGGACAAGG 
TGTGAGGACA CTACCAGGAG AAGGATGACA TCCCCGTTTA GAAGATTTAG GGAGAGAACG 
CTTTCAAGGG AGAGACTAGT CAACAACCAA AAAGAGGATG CACATCACAA TCAAGCTACA 
GACAGCTGTG AGAAAGTGAA GGATGTTGGA AGCAACATCA CAGATGAGAG AGGGAATGCC 
ATCTACGACT CGAGTAGCCA AGGCCACAGT ACCGCGCTCA GCTGCTTCTG TACGCGGTTC 
AAGACTAAAA GGAGAAAGAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_030245698.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101558.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X11, mRNA.

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CloneID	OMu85671
Clone ID Related Accession (Same CDS sequence)	XM_030245698.1
Accession Version	XM_030245698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG TTGGCAGTAT CCTATCTGAT 
CACAGCATCC TGTTGCGTCT CTCAGGGTCT TCCTCATCCC CTCCCCGTGA GCCCAGCACC 
AAGATCAGTG GCCAGAGCTG CCCCGGAATA GGTGGGGTTT ACTTGCAAAA GAAAATCCTT 
CAGATTACTG GGAGCACAGC CAAAAGAACA GACAGGACCA AGAAGGCCAC TGAGGAGAAT 
AGAGACAGGA CAAGGTGTGA GGACACTACC AGGAGAAGGA TGACATCCCC GTTTAGAAGA 
TTTAGGGAGA GAACGCTTTC AAGGGAGAGA CTAGTCAACA ACCAAAAAGA GGATGCACAT 
CACAATCAAG CTACAGACAG CTGTGAGAAA GTGAAGGATG TTGGAAGCAA CATCACAGAT 
GAGAGAGGGA ATGCCATCTA CGACTCGAGT AGCCAAGGCC ACAGTACCGC GCTCAGCTGC 
TTCTGTACGC GGTTCAAGAC TAAAAGGAGA AAGACTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101558.1
CDS	206..4447
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKVGSILSDHSILLRLSGSSSSPPREPSTKISGQSCPGIGGVYLQKKILQITGSTAKRTDRTKKATEENRDRTRCEDTTRRRMTSPFRRFRERTLSRERLVNNQKEDAHHNQATDSCEKVKDVGSNITDERGNAIYDSSSQGHSTALSCFCTRFKTKRRKTL

Target ORF information:

RefSeq Version	XM_030245698.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245698.1

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG TTGGCAGTAT CCTATCTGAT 
CACAGCATCC TGTTGCGTCT CTCAGGGTCT TCCTCATCCC CTCCCCGTGA GCCCAGCACC 
AAGATCAGTG GCCAGAGCTG CCCCGGAATA GGTGGGGTTT ACTTGCAAAA GAAAATCCTT 
CAGATTACTG GGAGCACAGC CAAAAGAACA GACAGGACCA AGAAGGCCAC TGAGGAGAAT 
AGAGACAGGA CAAGGTGTGA GGACACTACC AGGAGAAGGA TGACATCCCC GTTTAGAAGA 
TTTAGGGAGA GAACGCTTTC AAGGGAGAGA CTAGTCAACA ACCAAAAAGA GGATGCACAT 
CACAATCAAG CTACAGACAG CTGTGAGAAA GTGAAGGATG TTGGAAGCAA CATCACAGAT 
GAGAGAGGGA ATGCCATCTA CGACTCGAGT AGCCAAGGCC ACAGTACCGC GCTCAGCTGC 
TTCTGTACGC GGTTCAAGAC TAAAAGGAGA AAGACTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532560.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532623.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45796
Clone ID Related Accession (Same CDS sequence)	XM_006532560.4 , XM_006532560.3
Accession Version	XM_006532560.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532560.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT 
GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT 
GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG 
GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG 
CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG 
ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC 
TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC 
CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC 
TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC 
CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT 
GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT 
GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA 
GACAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AAGGTGATAG GTATAGGGAG 
CCACCGCCCA CTCCTCCAGG ATATCTGGGA ATTTCTTTAG CGGACCTAAA GGAAGGACCC 
CACCCGCACC TAAAACCTCC AGACTATAGT GTGGCAGTAC AGAGATCAAA GATGATGCTT 
AACAGCCTGT CTAGACTGCC GCCAGCTCCT CCCAGTAGCC ACACCTCGGC CTGGGTTCCC 
TCGAAGATTG GATCCCAGCC TCAAAGGCAT AGCCATCCCA AGCTAGCAGA TGTGGCTGAT 
GCAGATAGCG AAGCAGATGA AAACGAGCAG GTGTCAGCAG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532623.1
CDS	242..5086
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532560.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532560.4

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT 
GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT 
GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG 
GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG 
CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG 
ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC 
TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC 
CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC 
TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC 
CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT 
GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT 
GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA 
GACAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AAGGTGATAG GTATAGGGAG 
CCACCGCCCA CTCCTCCAGG ATATCTGGGA ATTTCTTTAG CGGACCTAAA GGAAGGACCC 
CACCCGCACC TAAAACCTCC AGACTATAGT GTGGCAGTAC AGAGATCAAA GATGATGCTT 
AACAGCCTGT CTAGACTGCC GCCAGCTCCT CCCAGTAGCC ACACCTCGGC CTGGGTTCCC 
TCGAAGATTG GATCCCAGCC TCAAAGGCAT AGCCATCCCA AGCTAGCAGA TGTGGCTGAT 
GCAGATAGCG AAGCAGATGA AAACGAGCAG GTGTCAGCAG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532561.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532624.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA.

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CloneID	OMu45797
Clone ID Related Accession (Same CDS sequence)	XM_006532561.3 , XM_006532561.4
Accession Version	XM_006532561.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532561.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC 
AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC 
AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA 
CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG 
CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA 
TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG 
CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC 
CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA 
GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC 
TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC 
ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG 
TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC 
TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC 
CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT 
CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT 
GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA 
CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC 
TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG 
CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG 
ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC 
AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT 
GTGTACTGTG TCACCTCACC CAAGAAAGGT GATAGGTATA GGGAGCCACC GCCCACTCCT 
CCAGGATATC TGGGAATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCC GCACCTAAAA 
CCTCCAGACT ATAGTGTGGC AGTACAGAGA TCAAAGATGA TGCTTAACAG CCTGTCTAGA 
CTGCCGCCAG CTCCTCCCAG TAGCCACACC TCGGCCTGGG TTCCCTCGAA GATTGGATCC 
CAGCCTCAAA GGCATAGCCA TCCCAAGCTA GCAGATGTGG CTGATGCAGA TAGCGAAGCA 
GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532624.1
CDS	206..5035
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532561.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532561.4

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC 
AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC 
AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA 
CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG 
CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA 
TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG 
CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC 
CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA 
GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC 
TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC 
ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG 
TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC 
TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC 
CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT 
CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT 
GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA 
CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC 
TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG 
CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG 
ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC 
AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT 
GTGTACTGTG TCACCTCACC CAAGAAAGGT GATAGGTATA GGGAGCCACC GCCCACTCCT 
CCAGGATATC TGGGAATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCC GCACCTAAAA 
CCTCCAGACT ATAGTGTGGC AGTACAGAGA TCAAAGATGA TGCTTAACAG CCTGTCTAGA 
CTGCCGCCAG CTCCTCCCAG TAGCCACACC TCGGCCTGGG TTCCCTCGAA GATTGGATCC 
CAGCCTCAAA GGCATAGCCA TCCCAAGCTA GCAGATGTGG CTGATGCAGA TAGCGAAGCA 
GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532562.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532625.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA.

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CloneID	OMu45798
Clone ID Related Accession (Same CDS sequence)	XM_006532562.3 , XM_006532562.2
Accession Version	XM_006532562.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532562.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAA GAGCACGCTA TGAGAGATAC AGTGGCAATC AAATGCTTTT TTGTTCAGAA 
ACTATTGCCA GATGTTGGTA TATCCTGCTT TCTGGATCTG TACTTGTAAA AGATTCTATG 
GTCCTTCCTC CTTGCAGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT 
GTGTTAGAAC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGACAGTATT 
TTACAAAGAG AAATTCCTGC CAGACAGTCT CGACGAAGGT TTCGGAAAAT TAACTATAAA 
GGAGAACGCC AAACCATAAT AGATGGAGTG GATATTAATA ACTACCTTTC GCTTCCAGCT 
GATCTTACCA AGATGCACCT CACAGACAAC CCTCATCCAC AGGTGACGCA TGTGTCTTCT 
AGTCAGTCTG GCTGTAGCAT TGCCAGTGAC TCTGGGAGCA GCAGTTTATC CGATATCTAT 
CAGGCTACAG AGAGTGAAGT AGGAGATGTA GATTTAACAC GCCTTCCAGA AGGACCTGTT 
GATTCTGAGG ATGAAGAGGA GGAGGAAGAA GAGATTGATA GAACAGATCC ACTGCAGGGT 
CGAGATCTTG TTCGGGAGTG TCTTGAAAAG GAACCCGCAG ACAAAACTGA TGATGATGTT 
GAACAGTTAC TTGAGTTCAT GCACCAGCTG CCTGCCTTTG CGAACATGAC CATGTCTGTA 
AGGAGAGAAC TCTGCTCCGT CATGGTTTTT GAAGTAGTAG AGCAGGCTGG GGCTGTTATT 
CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTCATTT TAAATGGCAC TGTAGAAATT 
AGTCATCCAG ATGGGAAAAT AGAAAATCTC TTCATGGGAA ATAGTTTTGG GATTGTCCCC 
ACGCTGGATA AGCAGCACAT GCATGGAGCT GTCAGGACCA AAGTCGATGA TTGTCAGTTT 
GTCTGCATAG CTCAACAAGA CTATTGGAGA ATTTTAAATC ATGTGGAAAA AAATACTCAC 
AAAGTTGAGG AAGAGGGAGA GATTGTCATG GTACATGAGC ACCGTGAGCT AGACCGGAGC 
GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTG 
ATAGAAGAAC ATTCTATTGT GGACCCAACA TACATAGAAG ATTTCCTCTT AACTTACAGG 
ACATTTCTTG AAACTCCTTT GGATGTTGGG ATCAAGCTTT TGGAATGGTT TAAGATTGAC 
AACCTAAGGG ATAAGGTGAC ACGGATTGTA CTCTTATGGG TAAATAATCA TTTTAATGAT 
TTTGAAGGTG ATCCTGCTAT GACTCAATTT CTAGAAGAAT TTGAAAGAAA TTTGGAAGAT 
ACAAAAATGA ATGGTCACCT CAGGTTATTG AATATTGCCT GTGCTGCAAA GGCTAAGTGG 
AGGCAAGTTG TACTGCAGAA GGCGTCCCGT GAGTCCCCGC TGCACTTCTG CCTTACTGGG 
GGCAGTGAGA AGGGATTTGG AGTCTTTGTT GAAGAAGTAG AATCTGGTAG CAAAGCTGCT 
GACGCAGGAC TGAAAAGGGG TGATCAGGTA ATGGAAGTCA ATGGACAAAA CTTTGAGAAT 
ATTACACTTG CTAAAGCCCT TGAAATTTTG AGAAATAATA CTCATCTTGC ACTTACTGTG 
AAGACCAACA TTTTTGTGTT CAAAGAATTG CTCAGTAGGA CTGAACAAGA GAAATCTGGT 
GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT TCAAGATGTC 
CCAGGGGATA TGGAGCAGGC ACCACAGGAG AAAGGAAATA AGAAAATTAA AGCAAATACC 
GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG 
CCTCCAAAAC TATTTAGTGA CGGAGGCCTG AGTCAATCAC AAGATGACAG CATTGTGGGA 
ACCAGGCACT GTAGGCACAG CCTGGCTATA ATGCCAATTC CCGGAACACT TTCATCCAGC 
AGCCCAGATC TCTTACAGCC TACCACTAGC ATGTTGGACT TTTCCAATCC TTCAGCTGTT 
GGTTTTTACT ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC 
TACATTATCA TCAGTAAGGA TACCACAGCT AAAGAAGTGG TCTGCCAAGC TGTACAGGAA 
TTCGGTTTGA CTGGTGCATC TGACACATAC TCCCTCTGTG AAGTGTCTGT TACTCCTGAG 
GGTGTTATAA AGCAGAGAAG ACTTCCGGAC CAGTTCTCCA AATTAGCTGA TAGAATTCAA 
CTCAATGGAA GGTATTACCT AAAAAACAAC ATGGAAACAG AAACATTGTG TTCTGATGAA 
GATGCTCAGG AACTACTTAA GGAAAGCCAG CTCTCCATGC TGCAGCTCAG TACCATCGAG 
GTCGCCACCC AGCTCTCCAT GAGAGACTTT GATTTATTTC GTAACATTGA GCCTACTGAA 
TACATTGATG ATCTTTTTAA ATTAGATTCC AAAACAGGAA ATACTCACTT GAAACAATTT 
GAGGATATTG TAAACCAAGA GACATTCTGG GTTGCCTCAG AGATTTTAAG TGAGTCAAAT 
CAGCTGAAAA GAATGAAGAT TATTAAACAT TTTATCAAAA TTGCTCTTCA TTGCCGAGAA 
TGCAAGAATT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ACCGGTAGCA 
CGACTCAGAG GCACTTGGGA AAAGTTGCCA AGCAAATATG AGAAACATCT TCAAGATCTA 
CAAGACCTTT TTGATCCATC TAGAAATATG GCAAAATACA GAAATATTCT TAGTAGTCAA 
AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTC 
CATGAAGGAA ATGACTCCAA AGTAGATGGC TTAGTAAACT TCGAGAAGCT AAGAATGATT 
GCCAAGGAAA TCCGTCATAT TATTCGAATG ACTTCTGCCA ACATGGACCC TGCCATGATG 
TTCCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT 
GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT 
CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC 
TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA 
GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA 
AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC 
GAGCAGGTGT CAGCAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532625.1
CDS	234..4934
Translation	MSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532562.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532562.3

ORF Insert Sequence:

ATGTCTACAA GAGCACGCTA TGAGAGATAC AGTGGCAATC AAATGCTTTT TTGTTCAGAA 
ACTATTGCCA GATGTTGGTA TATCCTGCTT TCTGGATCTG TACTTGTAAA AGATTCTATG 
GTCCTTCCTC CTTGCAGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT 
GTGTTAGAAC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGACAGTATT 
TTACAAAGAG AAATTCCTGC CAGACAGTCT CGACGAAGGT TTCGGAAAAT TAACTATAAA 
GGAGAACGCC AAACCATAAT AGATGGAGTG GATATTAATA ACTACCTTTC GCTTCCAGCT 
GATCTTACCA AGATGCACCT CACAGACAAC CCTCATCCAC AGGTGACGCA TGTGTCTTCT 
AGTCAGTCTG GCTGTAGCAT TGCCAGTGAC TCTGGGAGCA GCAGTTTATC CGATATCTAT 
CAGGCTACAG AGAGTGAAGT AGGAGATGTA GATTTAACAC GCCTTCCAGA AGGACCTGTT 
GATTCTGAGG ATGAAGAGGA GGAGGAAGAA GAGATTGATA GAACAGATCC ACTGCAGGGT 
CGAGATCTTG TTCGGGAGTG TCTTGAAAAG GAACCCGCAG ACAAAACTGA TGATGATGTT 
GAACAGTTAC TTGAGTTCAT GCACCAGCTG CCTGCCTTTG CGAACATGAC CATGTCTGTA 
AGGAGAGAAC TCTGCTCCGT CATGGTTTTT GAAGTAGTAG AGCAGGCTGG GGCTGTTATT 
CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTCATTT TAAATGGCAC TGTAGAAATT 
AGTCATCCAG ATGGGAAAAT AGAAAATCTC TTCATGGGAA ATAGTTTTGG GATTGTCCCC 
ACGCTGGATA AGCAGCACAT GCATGGAGCT GTCAGGACCA AAGTCGATGA TTGTCAGTTT 
GTCTGCATAG CTCAACAAGA CTATTGGAGA ATTTTAAATC ATGTGGAAAA AAATACTCAC 
AAAGTTGAGG AAGAGGGAGA GATTGTCATG GTACATGAGC ACCGTGAGCT AGACCGGAGC 
GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTG 
ATAGAAGAAC ATTCTATTGT GGACCCAACA TACATAGAAG ATTTCCTCTT AACTTACAGG 
ACATTTCTTG AAACTCCTTT GGATGTTGGG ATCAAGCTTT TGGAATGGTT TAAGATTGAC 
AACCTAAGGG ATAAGGTGAC ACGGATTGTA CTCTTATGGG TAAATAATCA TTTTAATGAT 
TTTGAAGGTG ATCCTGCTAT GACTCAATTT CTAGAAGAAT TTGAAAGAAA TTTGGAAGAT 
ACAAAAATGA ATGGTCACCT CAGGTTATTG AATATTGCCT GTGCTGCAAA GGCTAAGTGG 
AGGCAAGTTG TACTGCAGAA GGCGTCCCGT GAGTCCCCGC TGCACTTCTG CCTTACTGGG 
GGCAGTGAGA AGGGATTTGG AGTCTTTGTT GAAGAAGTAG AATCTGGTAG CAAAGCTGCT 
GACGCAGGAC TGAAAAGGGG TGATCAGGTA ATGGAAGTCA ATGGACAAAA CTTTGAGAAT 
ATTACACTTG CTAAAGCCCT TGAAATTTTG AGAAATAATA CTCATCTTGC ACTTACTGTG 
AAGACCAACA TTTTTGTGTT CAAAGAATTG CTCAGTAGGA CTGAACAAGA GAAATCTGGT 
GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT TCAAGATGTC 
CCAGGGGATA TGGAGCAGGC ACCACAGGAG AAAGGAAATA AGAAAATTAA AGCAAATACC 
GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG 
CCTCCAAAAC TATTTAGTGA CGGAGGCCTG AGTCAATCAC AAGATGACAG CATTGTGGGA 
ACCAGGCACT GTAGGCACAG CCTGGCTATA ATGCCAATTC CCGGAACACT TTCATCCAGC 
AGCCCAGATC TCTTACAGCC TACCACTAGC ATGTTGGACT TTTCCAATCC TTCAGCTGTT 
GGTTTTTACT ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC 
TACATTATCA TCAGTAAGGA TACCACAGCT AAAGAAGTGG TCTGCCAAGC TGTACAGGAA 
TTCGGTTTGA CTGGTGCATC TGACACATAC TCCCTCTGTG AAGTGTCTGT TACTCCTGAG 
GGTGTTATAA AGCAGAGAAG ACTTCCGGAC CAGTTCTCCA AATTAGCTGA TAGAATTCAA 
CTCAATGGAA GGTATTACCT AAAAAACAAC ATGGAAACAG AAACATTGTG TTCTGATGAA 
GATGCTCAGG AACTACTTAA GGAAAGCCAG CTCTCCATGC TGCAGCTCAG TACCATCGAG 
GTCGCCACCC AGCTCTCCAT GAGAGACTTT GATTTATTTC GTAACATTGA GCCTACTGAA 
TACATTGATG ATCTTTTTAA ATTAGATTCC AAAACAGGAA ATACTCACTT GAAACAATTT 
GAGGATATTG TAAACCAAGA GACATTCTGG GTTGCCTCAG AGATTTTAAG TGAGTCAAAT 
CAGCTGAAAA GAATGAAGAT TATTAAACAT TTTATCAAAA TTGCTCTTCA TTGCCGAGAA 
TGCAAGAATT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ACCGGTAGCA 
CGACTCAGAG GCACTTGGGA AAAGTTGCCA AGCAAATATG AGAAACATCT TCAAGATCTA 
CAAGACCTTT TTGATCCATC TAGAAATATG GCAAAATACA GAAATATTCT TAGTAGTCAA 
AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTC 
CATGAAGGAA ATGACTCCAA AGTAGATGGC TTAGTAAACT TCGAGAAGCT AAGAATGATT 
GCCAAGGAAA TCCGTCATAT TATTCGAATG ACTTCTGCCA ACATGGACCC TGCCATGATG 
TTCCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT 
GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT 
CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC 
TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA 
GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA 
AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC 
GAGCAGGTGT CAGCAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532563.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532626.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA.

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CloneID	OMu45799
Clone ID Related Accession (Same CDS sequence)	XM_006532563.4 , XM_006532563.3 , XM_030245697.1
Accession Version	XM_006532563.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532563.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA 
GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC 
ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT 
GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA 
GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG 
GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT 
CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG 
CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC 
ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT 
GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA 
GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG 
CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC 
TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG 
ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT 
GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG 
GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG 
GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA 
CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC 
AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG 
GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA 
GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT 
GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT 
GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC 
AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA 
CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC 
GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC 
CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT 
ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT 
CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT 
ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT 
GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA 
CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA 
AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG 
GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG 
AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA 
TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG 
ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT 
ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG 
TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA 
AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT 
AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT 
CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA 
GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT 
ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG 
TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG 
GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT 
GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA 
GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA 
AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG 
TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT 
CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG 
CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA 
CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT 
CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT 
GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC 
TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG 
CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT 
GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT 
CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG 
GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA 
AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA 
GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC 
CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA 
CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA 
CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT 
GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT 
GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC 
CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA 
TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC 
TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA 
GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532626.1
CDS	456..5015
Translation	MLVYPAFWICTCKRFYGPSSLQVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532563.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532563.4

ORF Insert Sequence:

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA 
GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC 
ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT 
GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA 
GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG 
GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT 
CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG 
CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC 
ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT 
GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA 
GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG 
CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC 
TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG 
ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT 
GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG 
GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG 
GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA 
CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC 
AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG 
GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA 
GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT 
GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT 
GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC 
AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA 
CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC 
GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC 
CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT 
ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT 
CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT 
ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT 
GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA 
CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA 
AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG 
GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG 
AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA 
TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG 
ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT 
ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG 
TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA 
AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT 
AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT 
CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA 
GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT 
ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG 
TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG 
GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT 
GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA 
GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA 
AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG 
TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT 
CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG 
CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA 
CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT 
CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT 
GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC 
TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG 
CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT 
GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT 
CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG 
GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA 
AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA 
GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC 
CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA 
CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA 
CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT 
GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT 
GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC 
CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA 
TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC 
TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA 
GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_030245697.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101557.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA.

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CloneID	OMu45799
Clone ID Related Accession (Same CDS sequence)	XM_006532563.4 , XM_006532563.3 , XM_030245697.1
Accession Version	XM_030245697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA 
GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC 
ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT 
GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA 
GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG 
GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT 
CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG 
CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC 
ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT 
GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA 
GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG 
CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC 
TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG 
ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT 
GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG 
GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG 
GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA 
CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC 
AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG 
GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA 
GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT 
GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT 
GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC 
AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA 
CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC 
GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC 
CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT 
ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT 
CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT 
ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT 
GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA 
CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA 
AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG 
GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG 
AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA 
TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG 
ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT 
ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG 
TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA 
AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT 
AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT 
CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA 
GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT 
ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG 
TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG 
GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT 
GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA 
GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA 
AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG 
TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT 
CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG 
CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA 
CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT 
CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT 
GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC 
TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG 
CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT 
GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT 
CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG 
GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA 
AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA 
GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC 
CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA 
CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA 
CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT 
GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT 
GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC 
CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA 
TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC 
TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA 
GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101557.1
CDS	385..4944
Translation	MLVYPAFWICTCKRFYGPSSLQVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_030245697.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245697.1

ORF Insert Sequence:

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA 
GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC 
ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT 
GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA 
GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG 
GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT 
CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG 
CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC 
ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT 
GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA 
GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG 
CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC 
TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG 
ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT 
GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG 
GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG 
GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA 
CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC 
AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG 
GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA 
GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT 
GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT 
GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC 
AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA 
CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC 
GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC 
CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT 
ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT 
CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT 
ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT 
GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA 
CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA 
AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG 
GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG 
AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA 
TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG 
ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT 
ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG 
TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA 
AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT 
AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT 
CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA 
GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT 
ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG 
TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG 
GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT 
GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA 
GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA 
AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG 
TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT 
CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG 
CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA 
CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT 
CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT 
GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC 
TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG 
CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT 
GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT 
CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG 
GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA 
AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA 
GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC 
CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA 
CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA 
CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT 
GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT 
GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC 
CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA 
TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC 
TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA 
GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532564.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532627.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu45800
Clone ID Related Accession (Same CDS sequence)	XM_006532564.3 , XM_006532564.4
Accession Version	XM_006532564.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532564.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC 
AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC 
AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA 
CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG 
CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA 
TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG 
CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC 
CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA 
GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC 
TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC 
ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG 
TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC 
TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC 
CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT 
CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT 
GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA 
CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC 
TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG 
CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG 
ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC 
AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT 
GAAAACGAGC AGGTGTCAGC AGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532627.1
CDS	206..4732
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIENEQVSAV

Target ORF information:

RefSeq Version	XM_006532564.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532564.4

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC 
AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC 
AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA 
CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG 
CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA 
TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG 
CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC 
CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA 
GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC 
TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC 
ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG 
TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC 
TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC 
CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT 
CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT 
GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA 
CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC 
TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG 
CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG 
ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC 
AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT 
GAAAACGAGC AGGTGTCAGC AGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_017314348.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169837.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA.

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CloneID	OMu77780
Clone ID Related Accession (Same CDS sequence)	XM_017314348.1 , XM_017314348.2
Accession Version	XM_017314348.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGAAAACGAG 
CAGGTGTCAG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169837.1
CDS	252..4769
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIENEQVSAV

Target ORF information:

RefSeq Version	XM_017314348.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314348.1

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGAAAACGAG 
CAGGTGTCAG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_017314348.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169837.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA.

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CloneID	OMu77780
Clone ID Related Accession (Same CDS sequence)	XM_017314348.1 , XM_017314348.2
Accession Version	XM_017314348.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGAAAACGAG 
CAGGTGTCAG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169837.1
CDS	206..4723
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIENEQVSAV

Target ORF information:

RefSeq Version	XM_017314348.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314348.2

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGAAAACGAG 
CAGGTGTCAG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( NM_175258.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780467.2 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 2, mRNA.

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CloneID	OMu19298
Clone ID Related Accession (Same CDS sequence)	NM_175258.4
Accession Version	NM_175258.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK167055.1, AK143331.1, AK220459.1 and AW493254.1. On Nov 16, 2011 this sequence version replaced NM_175258.3. Transcript Variant: This variant (2) lacks an in-frame coding exon, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT 
GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA 
CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA 
GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC 
TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA 
TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG 
GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC 
CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT 
AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA 
TCTCCTCAGA ACTCCCCACG CAAAGGTTAT ACACTCACAC CATCAAGTAA ATGTGACAAC 
TTGTCTGACT CCAGCCACAG TGAAATCTCC TCTCGGTCCA GCATTGTGAG CAATGGTTCT 
GTTGACTCCA TGTCTGCAGC TGGGCAGGAT GAACGCTGTT CCTCCCACAG TCTGGCAGTT 
CCTGAGCCCA CAGGGGCATT GGAAAAGACA GATCACCCTT CCGGGATCTC AGACCACAGT 
CAGCTTGCTC ACGGGTGGAT GCTGTCAAAG CCGTGTCTTA TCAAGGGTGT AGCTGTCTCA 
TCTTCTCTGA GCAGCGAAGA GATGTCTCAT GAGCATGTTG TGCTGGAAGC AGCTGACAGT 
GGGCGGGGCA GTTGGACTTC CTGTTCCAGC AGCTCCCATG ACAACTTCCA AAGCCTTCAA 
AATCAAAAAA GCTGGGACTT CCTGAATTCT TACAGACACA TGCATTTAGA TGACCCTATT 
GCAGAAGTTG AGCCCACTGA CTGTGAGCCC TGTGCCTGTC CTAAAGGCTG CTCAAGAACT 
TGTGGCCAGT GCAAAGGCAG CCTAGAGACG AACCAGCTGA GGCAGAGTTG GGCCTCCTCC 
AGCTCACTCT CGGACACATG TGAACCAAAC TACGGGACAG TTAAGCGGAG GGTGTTGGAA 
AGTGCACCAG CAGAGGCACC TGATGGCTTG GAGCCCAGGG ATACCACTGA CCCTGTTTAT 
AAAACTGTCA CTTCCAGTAC AGACAAGGGC TTGATTGTGT ACTGTGTCAC CTCACCCAAG 
AAAGGTGATA GGTATAGGGA GCCACCGCCC ACTCCTCCAG GATATCTGGG AATTTCTTTA 
GCGGACCTAA AGGAAGGACC CCACCCGCAC CTAAAACCTC CAGACTATAG TGTGGCAGTA 
CAGAGATCAA AGATGATGCT TAACAGCCTG TCTAGACTGC CGCCAGCTCC TCCCAGTAGC 
CACACCTCGG CCTGGGTTCC CTCGAAGATT GGATCCCAGC CTCAAAGGCA TAGCCATCCC 
AAGCTAGCAG ATGTGGCTGA TGCAGATAGC GAAGCAGATG AAAACGAGCA GGTGTCAGCA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780467.2
CDS	206..5011
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	NM_175258.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175258.4

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT 
GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA 
CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA 
GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC 
TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA 
TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG 
GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC 
CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT 
AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA 
TCTCCTCAGA ACTCCCCACG CAAAGGTTAT ACACTCACAC CATCAAGTAA ATGTGACAAC 
TTGTCTGACT CCAGCCACAG TGAAATCTCC TCTCGGTCCA GCATTGTGAG CAATGGTTCT 
GTTGACTCCA TGTCTGCAGC TGGGCAGGAT GAACGCTGTT CCTCCCACAG TCTGGCAGTT 
CCTGAGCCCA CAGGGGCATT GGAAAAGACA GATCACCCTT CCGGGATCTC AGACCACAGT 
CAGCTTGCTC ACGGGTGGAT GCTGTCAAAG CCGTGTCTTA TCAAGGGTGT AGCTGTCTCA 
TCTTCTCTGA GCAGCGAAGA GATGTCTCAT GAGCATGTTG TGCTGGAAGC AGCTGACAGT 
GGGCGGGGCA GTTGGACTTC CTGTTCCAGC AGCTCCCATG ACAACTTCCA AAGCCTTCAA 
AATCAAAAAA GCTGGGACTT CCTGAATTCT TACAGACACA TGCATTTAGA TGACCCTATT 
GCAGAAGTTG AGCCCACTGA CTGTGAGCCC TGTGCCTGTC CTAAAGGCTG CTCAAGAACT 
TGTGGCCAGT GCAAAGGCAG CCTAGAGACG AACCAGCTGA GGCAGAGTTG GGCCTCCTCC 
AGCTCACTCT CGGACACATG TGAACCAAAC TACGGGACAG TTAAGCGGAG GGTGTTGGAA 
AGTGCACCAG CAGAGGCACC TGATGGCTTG GAGCCCAGGG ATACCACTGA CCCTGTTTAT 
AAAACTGTCA CTTCCAGTAC AGACAAGGGC TTGATTGTGT ACTGTGTCAC CTCACCCAAG 
AAAGGTGATA GGTATAGGGA GCCACCGCCC ACTCCTCCAG GATATCTGGG AATTTCTTTA 
GCGGACCTAA AGGAAGGACC CCACCCGCAC CTAAAACCTC CAGACTATAG TGTGGCAGTA 
CAGAGATCAA AGATGATGCT TAACAGCCTG TCTAGACTGC CGCCAGCTCC TCCCAGTAGC 
CACACCTCGG CCTGGGTTCC CTCGAAGATT GGATCCCAGC CTCAAAGGCA TAGCCATCCC 
AAGCTAGCAG ATGTGGCTGA TGCAGATAGC GAAGCAGATG AAAACGAGCA GGTGTCAGCA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( NM_001252494.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239423.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19412
Clone ID Related Accession (Same CDS sequence)	NM_001252494.1
Accession Version	NM_001252494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK167055.1, BC141182.1, AK045540.1, BC145503.1, AK220459.1 and AW493254.1. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC141182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT 
GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT 
CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC 
TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA 
GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA 
AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC 
GAGCAGGTGT CAGCAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239423.1
CDS	206..5026
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	NM_001252494.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252494.1

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT 
GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT 
CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC 
TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA 
GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA 
AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC 
GAGCAGGTGT CAGCAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( NM_001252496.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239425.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 3, mRNA.

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CloneID	OMu19500
Clone ID Related Accession (Same CDS sequence)	NM_001252496.1
Accession Version	NM_001252496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK167055.1, BC145503.1, AK045540.1, AK220459.1 and AW493254.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC145503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT 
GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT 
GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG 
GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG 
CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG 
ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC 
TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC 
CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC 
TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC 
CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT 
GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT 
GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA 
GACAAGGGCT TGATTGAAAA CGAGCAGGTG TCAGCAGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239425.1
CDS	206..4747
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIENEQVSAV

Target ORF information:

RefSeq Version	NM_001252496.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252496.1

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT 
GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT 
GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG 
GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG 
CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG 
ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC 
TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC 
CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC 
TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC 
CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT 
GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT 
GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA 
GACAAGGGCT TGATTGAAAA CGAGCAGGTG TCAGCAGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532560.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532623.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA.

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CloneID	OMu45796
Clone ID Related Accession (Same CDS sequence)	XM_006532560.4 , XM_006532560.3
Accession Version	XM_006532560.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT 
GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT 
GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG 
GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG 
CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG 
ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC 
TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC 
CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC 
TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC 
CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT 
GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT 
GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA 
GACAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AAGGTGATAG GTATAGGGAG 
CCACCGCCCA CTCCTCCAGG ATATCTGGGA ATTTCTTTAG CGGACCTAAA GGAAGGACCC 
CACCCGCACC TAAAACCTCC AGACTATAGT GTGGCAGTAC AGAGATCAAA GATGATGCTT 
AACAGCCTGT CTAGACTGCC GCCAGCTCCT CCCAGTAGCC ACACCTCGGC CTGGGTTCCC 
TCGAAGATTG GATCCCAGCC TCAAAGGCAT AGCCATCCCA AGCTAGCAGA TGTGGCTGAT 
GCAGATAGCG AAGCAGATGA AAACGAGCAG GTGTCAGCAG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532623.1
CDS	251..5095
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532560.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532560.3

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGGTTATA CACTCACACC ATCAAGTAAA TGTGACAACT TGTCTGACTC CAGCCACAGT 
GAAATCTCCT CTCGGTCCAG CATTGTGAGC AATGGTTCTG TTGACTCCAT GTCTGCAGCT 
GGGCAGGATG AACGCTGTTC CTCCCACAGT CTGGCAGTTC CTGAGCCCAC AGGGGCATTG 
GAAAAGACAG ATCACCCTTC CGGGATCTCA GACCACAGTC AGCTTGCTCA CGGGTGGATG 
CTGTCAAAGC CGTGTCTTAT CAAGGGTGTA GCTGTCTCAT CTTCTCTGAG CAGCGAAGAG 
ATGTCTCATG AGCATGTTGT GCTGGAAGCA GCTGACAGTG GGCGGGGCAG TTGGACTTCC 
TGTTCCAGCA GCTCCCATGA CAACTTCCAA AGCCTTCAAA ATCAAAAAAG CTGGGACTTC 
CTGAATTCTT ACAGACACAT GCATTTAGAT GACCCTATTG CAGAAGTTGA GCCCACTGAC 
TGTGAGCCCT GTGCCTGTCC TAAAGGCTGC TCAAGAACTT GTGGCCAGTG CAAAGGCAGC 
CTAGAGACGA ACCAGCTGAG GCAGAGTTGG GCCTCCTCCA GCTCACTCTC GGACACATGT 
GAACCAAACT ACGGGACAGT TAAGCGGAGG GTGTTGGAAA GTGCACCAGC AGAGGCACCT 
GATGGCTTGG AGCCCAGGGA TACCACTGAC CCTGTTTATA AAACTGTCAC TTCCAGTACA 
GACAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AAGGTGATAG GTATAGGGAG 
CCACCGCCCA CTCCTCCAGG ATATCTGGGA ATTTCTTTAG CGGACCTAAA GGAAGGACCC 
CACCCGCACC TAAAACCTCC AGACTATAGT GTGGCAGTAC AGAGATCAAA GATGATGCTT 
AACAGCCTGT CTAGACTGCC GCCAGCTCCT CCCAGTAGCC ACACCTCGGC CTGGGTTCCC 
TCGAAGATTG GATCCCAGCC TCAAAGGCAT AGCCATCCCA AGCTAGCAGA TGTGGCTGAT 
GCAGATAGCG AAGCAGATGA AAACGAGCAG GTGTCAGCAG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532561.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532624.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA.

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CloneID	OMu45797
Clone ID Related Accession (Same CDS sequence)	XM_006532561.3 , XM_006532561.4
Accession Version	XM_006532561.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC 
AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC 
AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA 
CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG 
CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA 
TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG 
CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC 
CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA 
GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC 
TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC 
ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG 
TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC 
TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC 
CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT 
CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT 
GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA 
CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC 
TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG 
CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG 
ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC 
AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT 
GTGTACTGTG TCACCTCACC CAAGAAAGGT GATAGGTATA GGGAGCCACC GCCCACTCCT 
CCAGGATATC TGGGAATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCC GCACCTAAAA 
CCTCCAGACT ATAGTGTGGC AGTACAGAGA TCAAAGATGA TGCTTAACAG CCTGTCTAGA 
CTGCCGCCAG CTCCTCCCAG TAGCCACACC TCGGCCTGGG TTCCCTCGAA GATTGGATCC 
CAGCCTCAAA GGCATAGCCA TCCCAAGCTA GCAGATGTGG CTGATGCAGA TAGCGAAGCA 
GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532624.1
CDS	250..5079
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532561.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532561.3

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC 
AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC 
AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA 
CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG 
CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA 
TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG 
CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC 
CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA 
GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC 
TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC 
ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG 
TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC 
TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC 
CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT 
CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT 
GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA 
CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC 
TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG 
CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG 
ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC 
AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT 
GTGTACTGTG TCACCTCACC CAAGAAAGGT GATAGGTATA GGGAGCCACC GCCCACTCCT 
CCAGGATATC TGGGAATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCC GCACCTAAAA 
CCTCCAGACT ATAGTGTGGC AGTACAGAGA TCAAAGATGA TGCTTAACAG CCTGTCTAGA 
CTGCCGCCAG CTCCTCCCAG TAGCCACACC TCGGCCTGGG TTCCCTCGAA GATTGGATCC 
CAGCCTCAAA GGCATAGCCA TCCCAAGCTA GCAGATGTGG CTGATGCAGA TAGCGAAGCA 
GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532562.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532625.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA.

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CloneID	OMu45798
Clone ID Related Accession (Same CDS sequence)	XM_006532562.3 , XM_006532562.2
Accession Version	XM_006532562.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAA GAGCACGCTA TGAGAGATAC AGTGGCAATC AAATGCTTTT TTGTTCAGAA 
ACTATTGCCA GATGTTGGTA TATCCTGCTT TCTGGATCTG TACTTGTAAA AGATTCTATG 
GTCCTTCCTC CTTGCAGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT 
GTGTTAGAAC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGACAGTATT 
TTACAAAGAG AAATTCCTGC CAGACAGTCT CGACGAAGGT TTCGGAAAAT TAACTATAAA 
GGAGAACGCC AAACCATAAT AGATGGAGTG GATATTAATA ACTACCTTTC GCTTCCAGCT 
GATCTTACCA AGATGCACCT CACAGACAAC CCTCATCCAC AGGTGACGCA TGTGTCTTCT 
AGTCAGTCTG GCTGTAGCAT TGCCAGTGAC TCTGGGAGCA GCAGTTTATC CGATATCTAT 
CAGGCTACAG AGAGTGAAGT AGGAGATGTA GATTTAACAC GCCTTCCAGA AGGACCTGTT 
GATTCTGAGG ATGAAGAGGA GGAGGAAGAA GAGATTGATA GAACAGATCC ACTGCAGGGT 
CGAGATCTTG TTCGGGAGTG TCTTGAAAAG GAACCCGCAG ACAAAACTGA TGATGATGTT 
GAACAGTTAC TTGAGTTCAT GCACCAGCTG CCTGCCTTTG CGAACATGAC CATGTCTGTA 
AGGAGAGAAC TCTGCTCCGT CATGGTTTTT GAAGTAGTAG AGCAGGCTGG GGCTGTTATT 
CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTCATTT TAAATGGCAC TGTAGAAATT 
AGTCATCCAG ATGGGAAAAT AGAAAATCTC TTCATGGGAA ATAGTTTTGG GATTGTCCCC 
ACGCTGGATA AGCAGCACAT GCATGGAGCT GTCAGGACCA AAGTCGATGA TTGTCAGTTT 
GTCTGCATAG CTCAACAAGA CTATTGGAGA ATTTTAAATC ATGTGGAAAA AAATACTCAC 
AAAGTTGAGG AAGAGGGAGA GATTGTCATG GTACATGAGC ACCGTGAGCT AGACCGGAGC 
GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTG 
ATAGAAGAAC ATTCTATTGT GGACCCAACA TACATAGAAG ATTTCCTCTT AACTTACAGG 
ACATTTCTTG AAACTCCTTT GGATGTTGGG ATCAAGCTTT TGGAATGGTT TAAGATTGAC 
AACCTAAGGG ATAAGGTGAC ACGGATTGTA CTCTTATGGG TAAATAATCA TTTTAATGAT 
TTTGAAGGTG ATCCTGCTAT GACTCAATTT CTAGAAGAAT TTGAAAGAAA TTTGGAAGAT 
ACAAAAATGA ATGGTCACCT CAGGTTATTG AATATTGCCT GTGCTGCAAA GGCTAAGTGG 
AGGCAAGTTG TACTGCAGAA GGCGTCCCGT GAGTCCCCGC TGCACTTCTG CCTTACTGGG 
GGCAGTGAGA AGGGATTTGG AGTCTTTGTT GAAGAAGTAG AATCTGGTAG CAAAGCTGCT 
GACGCAGGAC TGAAAAGGGG TGATCAGGTA ATGGAAGTCA ATGGACAAAA CTTTGAGAAT 
ATTACACTTG CTAAAGCCCT TGAAATTTTG AGAAATAATA CTCATCTTGC ACTTACTGTG 
AAGACCAACA TTTTTGTGTT CAAAGAATTG CTCAGTAGGA CTGAACAAGA GAAATCTGGT 
GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT TCAAGATGTC 
CCAGGGGATA TGGAGCAGGC ACCACAGGAG AAAGGAAATA AGAAAATTAA AGCAAATACC 
GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG 
CCTCCAAAAC TATTTAGTGA CGGAGGCCTG AGTCAATCAC AAGATGACAG CATTGTGGGA 
ACCAGGCACT GTAGGCACAG CCTGGCTATA ATGCCAATTC CCGGAACACT TTCATCCAGC 
AGCCCAGATC TCTTACAGCC TACCACTAGC ATGTTGGACT TTTCCAATCC TTCAGCTGTT 
GGTTTTTACT ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC 
TACATTATCA TCAGTAAGGA TACCACAGCT AAAGAAGTGG TCTGCCAAGC TGTACAGGAA 
TTCGGTTTGA CTGGTGCATC TGACACATAC TCCCTCTGTG AAGTGTCTGT TACTCCTGAG 
GGTGTTATAA AGCAGAGAAG ACTTCCGGAC CAGTTCTCCA AATTAGCTGA TAGAATTCAA 
CTCAATGGAA GGTATTACCT AAAAAACAAC ATGGAAACAG AAACATTGTG TTCTGATGAA 
GATGCTCAGG AACTACTTAA GGAAAGCCAG CTCTCCATGC TGCAGCTCAG TACCATCGAG 
GTCGCCACCC AGCTCTCCAT GAGAGACTTT GATTTATTTC GTAACATTGA GCCTACTGAA 
TACATTGATG ATCTTTTTAA ATTAGATTCC AAAACAGGAA ATACTCACTT GAAACAATTT 
GAGGATATTG TAAACCAAGA GACATTCTGG GTTGCCTCAG AGATTTTAAG TGAGTCAAAT 
CAGCTGAAAA GAATGAAGAT TATTAAACAT TTTATCAAAA TTGCTCTTCA TTGCCGAGAA 
TGCAAGAATT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ACCGGTAGCA 
CGACTCAGAG GCACTTGGGA AAAGTTGCCA AGCAAATATG AGAAACATCT TCAAGATCTA 
CAAGACCTTT TTGATCCATC TAGAAATATG GCAAAATACA GAAATATTCT TAGTAGTCAA 
AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTC 
CATGAAGGAA ATGACTCCAA AGTAGATGGC TTAGTAAACT TCGAGAAGCT AAGAATGATT 
GCCAAGGAAA TCCGTCATAT TATTCGAATG ACTTCTGCCA ACATGGACCC TGCCATGATG 
TTCCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT 
GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT 
CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC 
TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA 
GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA 
AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC 
GAGCAGGTGT CAGCAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532625.1
CDS	323..5023
Translation	MSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532562.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532562.2

ORF Insert Sequence:

ATGTCTACAA GAGCACGCTA TGAGAGATAC AGTGGCAATC AAATGCTTTT TTGTTCAGAA 
ACTATTGCCA GATGTTGGTA TATCCTGCTT TCTGGATCTG TACTTGTAAA AGATTCTATG 
GTCCTTCCTC CTTGCAGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT 
GTGTTAGAAC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGACAGTATT 
TTACAAAGAG AAATTCCTGC CAGACAGTCT CGACGAAGGT TTCGGAAAAT TAACTATAAA 
GGAGAACGCC AAACCATAAT AGATGGAGTG GATATTAATA ACTACCTTTC GCTTCCAGCT 
GATCTTACCA AGATGCACCT CACAGACAAC CCTCATCCAC AGGTGACGCA TGTGTCTTCT 
AGTCAGTCTG GCTGTAGCAT TGCCAGTGAC TCTGGGAGCA GCAGTTTATC CGATATCTAT 
CAGGCTACAG AGAGTGAAGT AGGAGATGTA GATTTAACAC GCCTTCCAGA AGGACCTGTT 
GATTCTGAGG ATGAAGAGGA GGAGGAAGAA GAGATTGATA GAACAGATCC ACTGCAGGGT 
CGAGATCTTG TTCGGGAGTG TCTTGAAAAG GAACCCGCAG ACAAAACTGA TGATGATGTT 
GAACAGTTAC TTGAGTTCAT GCACCAGCTG CCTGCCTTTG CGAACATGAC CATGTCTGTA 
AGGAGAGAAC TCTGCTCCGT CATGGTTTTT GAAGTAGTAG AGCAGGCTGG GGCTGTTATT 
CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTCATTT TAAATGGCAC TGTAGAAATT 
AGTCATCCAG ATGGGAAAAT AGAAAATCTC TTCATGGGAA ATAGTTTTGG GATTGTCCCC 
ACGCTGGATA AGCAGCACAT GCATGGAGCT GTCAGGACCA AAGTCGATGA TTGTCAGTTT 
GTCTGCATAG CTCAACAAGA CTATTGGAGA ATTTTAAATC ATGTGGAAAA AAATACTCAC 
AAAGTTGAGG AAGAGGGAGA GATTGTCATG GTACATGAGC ACCGTGAGCT AGACCGGAGC 
GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTG 
ATAGAAGAAC ATTCTATTGT GGACCCAACA TACATAGAAG ATTTCCTCTT AACTTACAGG 
ACATTTCTTG AAACTCCTTT GGATGTTGGG ATCAAGCTTT TGGAATGGTT TAAGATTGAC 
AACCTAAGGG ATAAGGTGAC ACGGATTGTA CTCTTATGGG TAAATAATCA TTTTAATGAT 
TTTGAAGGTG ATCCTGCTAT GACTCAATTT CTAGAAGAAT TTGAAAGAAA TTTGGAAGAT 
ACAAAAATGA ATGGTCACCT CAGGTTATTG AATATTGCCT GTGCTGCAAA GGCTAAGTGG 
AGGCAAGTTG TACTGCAGAA GGCGTCCCGT GAGTCCCCGC TGCACTTCTG CCTTACTGGG 
GGCAGTGAGA AGGGATTTGG AGTCTTTGTT GAAGAAGTAG AATCTGGTAG CAAAGCTGCT 
GACGCAGGAC TGAAAAGGGG TGATCAGGTA ATGGAAGTCA ATGGACAAAA CTTTGAGAAT 
ATTACACTTG CTAAAGCCCT TGAAATTTTG AGAAATAATA CTCATCTTGC ACTTACTGTG 
AAGACCAACA TTTTTGTGTT CAAAGAATTG CTCAGTAGGA CTGAACAAGA GAAATCTGGT 
GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT TCAAGATGTC 
CCAGGGGATA TGGAGCAGGC ACCACAGGAG AAAGGAAATA AGAAAATTAA AGCAAATACC 
GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG 
CCTCCAAAAC TATTTAGTGA CGGAGGCCTG AGTCAATCAC AAGATGACAG CATTGTGGGA 
ACCAGGCACT GTAGGCACAG CCTGGCTATA ATGCCAATTC CCGGAACACT TTCATCCAGC 
AGCCCAGATC TCTTACAGCC TACCACTAGC ATGTTGGACT TTTCCAATCC TTCAGCTGTT 
GGTTTTTACT ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC 
TACATTATCA TCAGTAAGGA TACCACAGCT AAAGAAGTGG TCTGCCAAGC TGTACAGGAA 
TTCGGTTTGA CTGGTGCATC TGACACATAC TCCCTCTGTG AAGTGTCTGT TACTCCTGAG 
GGTGTTATAA AGCAGAGAAG ACTTCCGGAC CAGTTCTCCA AATTAGCTGA TAGAATTCAA 
CTCAATGGAA GGTATTACCT AAAAAACAAC ATGGAAACAG AAACATTGTG TTCTGATGAA 
GATGCTCAGG AACTACTTAA GGAAAGCCAG CTCTCCATGC TGCAGCTCAG TACCATCGAG 
GTCGCCACCC AGCTCTCCAT GAGAGACTTT GATTTATTTC GTAACATTGA GCCTACTGAA 
TACATTGATG ATCTTTTTAA ATTAGATTCC AAAACAGGAA ATACTCACTT GAAACAATTT 
GAGGATATTG TAAACCAAGA GACATTCTGG GTTGCCTCAG AGATTTTAAG TGAGTCAAAT 
CAGCTGAAAA GAATGAAGAT TATTAAACAT TTTATCAAAA TTGCTCTTCA TTGCCGAGAA 
TGCAAGAATT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ACCGGTAGCA 
CGACTCAGAG GCACTTGGGA AAAGTTGCCA AGCAAATATG AGAAACATCT TCAAGATCTA 
CAAGACCTTT TTGATCCATC TAGAAATATG GCAAAATACA GAAATATTCT TAGTAGTCAA 
AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTC 
CATGAAGGAA ATGACTCCAA AGTAGATGGC TTAGTAAACT TCGAGAAGCT AAGAATGATT 
GCCAAGGAAA TCCGTCATAT TATTCGAATG ACTTCTGCCA ACATGGACCC TGCCATGATG 
TTCCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAT TGAGTCAAGG CAGCACAAAT 
TCTAACATGC TAGATGTCCA GGGAGGTGCT CACAAAAAAA GAGCACGCCG CAGCTCACTG 
CTGAATGCCA AGAAACTGTA TGAAGATGCC CAAATGGCAA GGAAAGTGAA ACAGTATCTT 
TCTAGTCTGG ACATAGACAC AGATGAGGAG AAGTTCCAGA TGATGTCACT GCAGTGGGAA 
CCTGCATATG GTACCTTGAC AAAGAACTTA ACTGAAAAAA GGTCAGCCAA ATCATCTGAG 
ATGTCTCCTG TACCATTGAG GTCAGTTGGC CAAACAGCTA AAGTCCACTT GCATCAGCCA 
CACAGAGTGA GCCAGGTGCT TCAGGTGCCA GCAGTTAACT TGCACCCCAT CCGGAAGAAA 
GGACAAGCCA AAGACCATGT GCTGAGTACA AGTTTACCTC AGAAAGGTTT AGGACCAACT 
GAGGAAGTGA GTGTTAAGAA ACACACAGAA GACACTATTT CTGTAGCGTC CTCTCTGCAC 
TCTAGCCCTC CTGCATCTCC TCAGAACTCC CCACGCAAAG GTTATACACT CACACCATCA 
AGTAAATGTG ACAACTTGTC TGACTCCAGC CACAGTGAAA TCTCCTCTCG GTCCAGCATT 
GTGAGCAATG GTTCTGTTGA CTCCATGTCT GCAGCTGGGC AGGATGAACG CTGTTCCTCC 
CACAGTCTGG CAGTTCCTGA GCCCACAGGG GCATTGGAAA AGACAGATCA CCCTTCCGGG 
ATCTCAGACC ACAGTCAGCT TGCTCACGGG TGGATGCTGT CAAAGCCGTG TCTTATCAAG 
GGTGTAGCTG TCTCATCTTC TCTGAGCAGC GAAGAGATGT CTCATGAGCA TGTTGTGCTG 
GAAGCAGCTG ACAGTGGGCG GGGCAGTTGG ACTTCCTGTT CCAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCAAAATCA AAAAAGCTGG GACTTCCTGA ATTCTTACAG ACACATGCAT 
TTAGATGACC CTATTGCAGA AGTTGAGCCC ACTGACTGTG AGCCCTGTGC CTGTCCTAAA 
GGCTGCTCAA GAACTTGTGG CCAGTGCAAA GGCAGCCTAG AGACGAACCA GCTGAGGCAG 
AGTTGGGCCT CCTCCAGCTC ACTCTCGGAC ACATGTGAAC CAAACTACGG GACAGTTAAG 
CGGAGGGTGT TGGAAAGTGC ACCAGCAGAG GCACCTGATG GCTTGGAGCC CAGGGATACC 
ACTGACCCTG TTTATAAAAC TGTCACTTCC AGTACAGACA AGGGCTTGAT TGTGTACTGT 
GTCACCTCAC CCAAGAAAGG TGATAGGTAT AGGGAGCCAC CGCCCACTCC TCCAGGATAT 
CTGGGAATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC CGCACCTAAA ACCTCCAGAC 
TATAGTGTGG CAGTACAGAG ATCAAAGATG ATGCTTAACA GCCTGTCTAG ACTGCCGCCA 
GCTCCTCCCA GTAGCCACAC CTCGGCCTGG GTTCCCTCGA AGATTGGATC CCAGCCTCAA 
AGGCATAGCC ATCCCAAGCT AGCAGATGTG GCTGATGCAG ATAGCGAAGC AGATGAAAAC 
GAGCAGGTGT CAGCAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532563.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532626.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA.

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CloneID	OMu45799
Clone ID Related Accession (Same CDS sequence)	XM_006532563.4 , XM_006532563.3 , XM_030245697.1
Accession Version	XM_006532563.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA 
GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC 
ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT 
GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA 
GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG 
GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT 
CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG 
CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC 
ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT 
GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA 
GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG 
CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC 
TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG 
ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT 
GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG 
GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG 
GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA 
CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC 
AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG 
GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA 
GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT 
GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT 
GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC 
AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA 
CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC 
GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC 
CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT 
ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT 
CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT 
ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT 
GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA 
CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA 
AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG 
GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG 
AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA 
TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG 
ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT 
ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG 
TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA 
AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT 
AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT 
CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA 
GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT 
ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG 
TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG 
GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT 
GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA 
GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA 
AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG 
TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT 
CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG 
CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA 
CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT 
CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT 
GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC 
TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG 
CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT 
GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT 
CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG 
GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA 
AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA 
GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC 
CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA 
CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA 
CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT 
GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT 
GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC 
CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA 
TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC 
TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA 
GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532626.1
CDS	465..5024
Translation	MLVYPAFWICTCKRFYGPSSLQVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532563.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532563.3

ORF Insert Sequence:

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT ACTTGTAAAA GATTCTATGG TCCTTCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GACAGTATTT TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GACGAAGGTT TCGGAAAATT AACTATAAAG GAGAACGCCA AACCATAATA 
GATGGAGTGG ATATTAATAA CTACCTTTCG CTTCCAGCTG ATCTTACCAA GATGCACCTC 
ACAGACAACC CTCATCCACA GGTGACGCAT GTGTCTTCTA GTCAGTCTGG CTGTAGCATT 
GCCAGTGACT CTGGGAGCAG CAGTTTATCC GATATCTATC AGGCTACAGA GAGTGAAGTA 
GGAGATGTAG ATTTAACACG CCTTCCAGAA GGACCTGTTG ATTCTGAGGA TGAAGAGGAG 
GAGGAAGAAG AGATTGATAG AACAGATCCA CTGCAGGGTC GAGATCTTGT TCGGGAGTGT 
CTTGAAAAGG AACCCGCAGA CAAAACTGAT GATGATGTTG AACAGTTACT TGAGTTCATG 
CACCAGCTGC CTGCCTTTGC GAACATGACC ATGTCTGTAA GGAGAGAACT CTGCTCCGTC 
ATGGTTTTTG AAGTAGTAGA GCAGGCTGGG GCTGTTATTC TTGAAGATGG GCAAGAGCTT 
GACTCATGGT ATGTCATTTT AAATGGCACT GTAGAAATTA GTCATCCAGA TGGGAAAATA 
GAAAATCTCT TCATGGGAAA TAGTTTTGGG ATTGTCCCCA CGCTGGATAA GCAGCACATG 
CATGGAGCTG TCAGGACCAA AGTCGATGAT TGTCAGTTTG TCTGCATAGC TCAACAAGAC 
TATTGGAGAA TTTTAAATCA TGTGGAAAAA AATACTCACA AAGTTGAGGA AGAGGGAGAG 
ATTGTCATGG TACATGAGCA CCGTGAGCTA GACCGGAGCG GAACCAGGAA AGGACACATT 
GTAATCAAGG CAACACCTGA GCGTCTCATC ATGCATTTGA TAGAAGAACA TTCTATTGTG 
GACCCAACAT ACATAGAAGA TTTCCTCTTA ACTTACAGGA CATTTCTTGA AACTCCTTTG 
GATGTTGGGA TCAAGCTTTT GGAATGGTTT AAGATTGACA ACCTAAGGGA TAAGGTGACA 
CGGATTGTAC TCTTATGGGT AAATAATCAT TTTAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAAGAATT TGAAAGAAAT TTGGAAGATA CAAAAATGAA TGGTCACCTC 
AGGTTATTGA ATATTGCCTG TGCTGCAAAG GCTAAGTGGA GGCAAGTTGT ACTGCAGAAG 
GCGTCCCGTG AGTCCCCGCT GCACTTCTGC CTTACTGGGG GCAGTGAGAA GGGATTTGGA 
GTCTTTGTTG AAGAAGTAGA ATCTGGTAGC AAAGCTGCTG ACGCAGGACT GAAAAGGGGT 
GATCAGGTAA TGGAAGTCAA TGGACAAAAC TTTGAGAATA TTACACTTGC TAAAGCCCTT 
GAAATTTTGA GAAATAATAC TCATCTTGCA CTTACTGTGA AGACCAACAT TTTTGTGTTC 
AAAGAATTGC TCAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCATTCAATT CAAGATGTCC CAGGGGATAT GGAGCAGGCA 
CCACAGGAGA AAGGAAATAA GAAAATTAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT ATTTAGTGAC 
GGAGGCCTGA GTCAATCACA AGATGACAGC ATTGTGGGAA CCAGGCACTG TAGGCACAGC 
CTGGCTATAA TGCCAATTCC CGGAACACTT TCATCCAGCA GCCCAGATCT CTTACAGCCT 
ACCACTAGCA TGTTGGACTT TTCCAATCCT TCAGCTGTTG GTTTTTACTA TATTCCTGAT 
CAAGTTATAA GAGTTTTCAA AGCAGATCAA CAAAGTTGCT ACATTATCAT CAGTAAGGAT 
ACCACAGCTA AAGAAGTGGT CTGCCAAGCT GTACAGGAAT TCGGTTTGAC TGGTGCATCT 
GACACATACT CCCTCTGTGA AGTGTCTGTT ACTCCTGAGG GTGTTATAAA GCAGAGAAGA 
CTTCCGGACC AGTTCTCCAA ATTAGCTGAT AGAATTCAAC TCAATGGAAG GTATTACCTA 
AAAAACAACA TGGAAACAGA AACATTGTGT TCTGATGAAG ATGCTCAGGA ACTACTTAAG 
GAAAGCCAGC TCTCCATGCT GCAGCTCAGT ACCATCGAGG TCGCCACCCA GCTCTCCATG 
AGAGACTTTG ATTTATTTCG TAACATTGAG CCTACTGAAT ACATTGATGA TCTTTTTAAA 
TTAGATTCCA AAACAGGAAA TACTCACTTG AAACAATTTG AGGATATTGT AAACCAAGAG 
ACATTCTGGG TTGCCTCAGA GATTTTAAGT GAGTCAAATC AGCTGAAAAG AATGAAGATT 
ATTAAACATT TTATCAAAAT TGCTCTTCAT TGCCGAGAAT GCAAGAATTT CAATTCCATG 
TTTGCAATAA TAAGTGGCTT GAACCTGGCA CCGGTAGCAC GACTCAGAGG CACTTGGGAA 
AAGTTGCCAA GCAAATATGA GAAACATCTT CAAGATCTAC AAGACCTTTT TGATCCATCT 
AGAAATATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT 
CCACTCTTCC CTGTTGTCAA GAAAGATATG ACATTTCTCC ATGAAGGAAA TGACTCCAAA 
GTAGATGGCT TAGTAAACTT CGAGAAGCTA AGAATGATTG CCAAGGAAAT CCGTCATATT 
ATTCGAATGA CTTCTGCCAA CATGGACCCT GCCATGATGT TCCGACAGAG GAAGAAGAGG 
TGGCGGAGTC TGGGGTCATT GAGTCAAGGC AGCACAAATT CTAACATGCT AGATGTCCAG 
GGAGGTGCTC ACAAAAAAAG AGCACGCCGC AGCTCACTGC TGAATGCCAA GAAACTGTAT 
GAAGATGCCC AAATGGCAAG GAAAGTGAAA CAGTATCTTT CTAGTCTGGA CATAGACACA 
GATGAGGAGA AGTTCCAGAT GATGTCACTG CAGTGGGAAC CTGCATATGG TACCTTGACA 
AAGAACTTAA CTGAAAAAAG GTCAGCCAAA TCATCTGAGA TGTCTCCTGT ACCATTGAGG 
TCAGTTGGCC AAACAGCTAA AGTCCACTTG CATCAGCCAC ACAGAGTGAG CCAGGTGCTT 
CAGGTGCCAG CAGTTAACTT GCACCCCATC CGGAAGAAAG GACAAGCCAA AGACCATGTG 
CTGAGTACAA GTTTACCTCA GAAAGGTTTA GGACCAACTG AGGAAGTGAG TGTTAAGAAA 
CACACAGAAG ACACTATTTC TGTAGCGTCC TCTCTGCACT CTAGCCCTCC TGCATCTCCT 
CAGAACTCCC CACGCAAAGG TTATACACTC ACACCATCAA GTAAATGTGA CAACTTGTCT 
GACTCCAGCC ACAGTGAAAT CTCCTCTCGG TCCAGCATTG TGAGCAATGG TTCTGTTGAC 
TCCATGTCTG CAGCTGGGCA GGATGAACGC TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG 
CCCACAGGGG CATTGGAAAA GACAGATCAC CCTTCCGGGA TCTCAGACCA CAGTCAGCTT 
GCTCACGGGT GGATGCTGTC AAAGCCGTGT CTTATCAAGG GTGTAGCTGT CTCATCTTCT 
CTGAGCAGCG AAGAGATGTC TCATGAGCAT GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG 
GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC CATGACAACT TCCAAAGCCT TCAAAATCAA 
AAAAGCTGGG ACTTCCTGAA TTCTTACAGA CACATGCATT TAGATGACCC TATTGCAGAA 
GTTGAGCCCA CTGACTGTGA GCCCTGTGCC TGTCCTAAAG GCTGCTCAAG AACTTGTGGC 
CAGTGCAAAG GCAGCCTAGA GACGAACCAG CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA 
CTCTCGGACA CATGTGAACC AAACTACGGG ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA 
CCAGCAGAGG CACCTGATGG CTTGGAGCCC AGGGATACCA CTGACCCTGT TTATAAAACT 
GTCACTTCCA GTACAGACAA GGGCTTGATT GTGTACTGTG TCACCTCACC CAAGAAAGGT 
GATAGGTATA GGGAGCCACC GCCCACTCCT CCAGGATATC TGGGAATTTC TTTAGCGGAC 
CTAAAGGAAG GACCCCACCC GCACCTAAAA CCTCCAGACT ATAGTGTGGC AGTACAGAGA 
TCAAAGATGA TGCTTAACAG CCTGTCTAGA CTGCCGCCAG CTCCTCCCAG TAGCCACACC 
TCGGCCTGGG TTCCCTCGAA GATTGGATCC CAGCCTCAAA GGCATAGCCA TCCCAAGCTA 
GCAGATGTGG CTGATGCAGA TAGCGAAGCA GATGAAAACG AGCAGGTGTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532564.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532627.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu45800
Clone ID Related Accession (Same CDS sequence)	XM_006532564.3 , XM_006532564.4
Accession Version	XM_006532564.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC 
AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC 
AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA 
CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG 
CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA 
TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG 
CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC 
CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA 
GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC 
TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC 
ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG 
TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC 
TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC 
CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT 
CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT 
GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA 
CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC 
TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG 
CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG 
ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC 
AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT 
GAAAACGAGC AGGTGTCAGC AGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532627.1
CDS	252..4778
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIENEQVSAV

Target ORF information:

RefSeq Version	XM_006532564.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532564.3

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCATT GAGTCAAGGC 
AGCACAAATT CTAACATGCT AGATGTCCAG GGAGGTGCTC ACAAAAAAAG AGCACGCCGC 
AGCTCACTGC TGAATGCCAA GAAACTGTAT GAAGATGCCC AAATGGCAAG GAAAGTGAAA 
CAGTATCTTT CTAGTCTGGA CATAGACACA GATGAGGAGA AGTTCCAGAT GATGTCACTG 
CAGTGGGAAC CTGCATATGG TACCTTGACA AAGAACTTAA CTGAAAAAAG GTCAGCCAAA 
TCATCTGAGA TGTCTCCTGT ACCATTGAGG TCAGTTGGCC AAACAGCTAA AGTCCACTTG 
CATCAGCCAC ACAGAGTGAG CCAGGTGCTT CAGGTGCCAG CAGTTAACTT GCACCCCATC 
CGGAAGAAAG GACAAGCCAA AGACCATGTG CTGAGTACAA GTTTACCTCA GAAAGGTTTA 
GGACCAACTG AGGAAGTGAG TGTTAAGAAA CACACAGAAG ACACTATTTC TGTAGCGTCC 
TCTCTGCACT CTAGCCCTCC TGCATCTCCT CAGAACTCCC CACGCAAAGG TTATACACTC 
ACACCATCAA GTAAATGTGA CAACTTGTCT GACTCCAGCC ACAGTGAAAT CTCCTCTCGG 
TCCAGCATTG TGAGCAATGG TTCTGTTGAC TCCATGTCTG CAGCTGGGCA GGATGAACGC 
TGTTCCTCCC ACAGTCTGGC AGTTCCTGAG CCCACAGGGG CATTGGAAAA GACAGATCAC 
CCTTCCGGGA TCTCAGACCA CAGTCAGCTT GCTCACGGGT GGATGCTGTC AAAGCCGTGT 
CTTATCAAGG GTGTAGCTGT CTCATCTTCT CTGAGCAGCG AAGAGATGTC TCATGAGCAT 
GTTGTGCTGG AAGCAGCTGA CAGTGGGCGG GGCAGTTGGA CTTCCTGTTC CAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCAAAATCAA AAAAGCTGGG ACTTCCTGAA TTCTTACAGA 
CACATGCATT TAGATGACCC TATTGCAGAA GTTGAGCCCA CTGACTGTGA GCCCTGTGCC 
TGTCCTAAAG GCTGCTCAAG AACTTGTGGC CAGTGCAAAG GCAGCCTAGA GACGAACCAG 
CTGAGGCAGA GTTGGGCCTC CTCCAGCTCA CTCTCGGACA CATGTGAACC AAACTACGGG 
ACAGTTAAGC GGAGGGTGTT GGAAAGTGCA CCAGCAGAGG CACCTGATGG CTTGGAGCCC 
AGGGATACCA CTGACCCTGT TTATAAAACT GTCACTTCCA GTACAGACAA GGGCTTGATT 
GAAAACGAGC AGGTGTCAGC AGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532565.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532628.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA.

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CloneID	OMu45801
Clone ID Related Accession (Same CDS sequence)	XM_006532565.3 , XM_006532565.4
Accession Version	XM_006532565.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532565.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGCT 
GTTGGTTTTT ACTATATTCC TGATCAAGTT ATAAGAGTTT TCAAAGCAGA TCAACAAAGT 
TGCTACATTA TCATCAGTAA GGATACCACA GCTAAAGAAG TGGTCTGCCA AGCTGTACAG 
GAATTCGGTT TGACTGGTGC ATCTGACACA TACTCCCTCT GTGAAGTGTC TGTTACTCCT 
GAGGGTGTTA TAAAGCAGAG AAGACTTCCG GACCAGTTCT CCAAATTAGC TGATAGAATT 
CAACTCAATG GAAGGTATTA CCTAAAAAAC AACATGGAAA CAGAAACATT GTGTTCTGAT 
GAAGATGCTC AGGAACTACT TAAGGAAAGC CAGCTCTCCA TGCTGCAGCT CAGTACCATC 
GAGGTCGCCA CCCAGCTCTC CATGAGAGAC TTTGATTTAT TTCGTAACAT TGAGCCTACT 
GAATACATTG ATGATCTTTT TAAATTAGAT TCCAAAACAG GAAATACTCA CTTGAAACAA 
TTTGAGGATA TTGTAAACCA AGAGACATTC TGGGTTGCCT CAGAGATTTT AAGTGAGTCA 
AATCAGCTGA AAAGAATGAA GATTATTAAA CATTTTATCA AAATTGCTCT TCATTGCCGA 
GAATGCAAGA ATTTCAATTC CATGTTTGCA ATAATAAGTG GCTTGAACCT GGCACCGGTA 
GCACGACTCA GAGGCACTTG GGAAAAGTTG CCAAGCAAAT ATGAGAAACA TCTTCAAGAT 
CTACAAGACC TTTTTGATCC ATCTAGAAAT ATGGCAAAAT ACAGAAATAT TCTTAGTAGT 
CAAAGCATGC AGCCTCCAAT TATTCCACTC TTCCCTGTTG TCAAGAAAGA TATGACATTT 
CTCCATGAAG GAAATGACTC CAAAGTAGAT GGCTTAGTAA ACTTCGAGAA GCTAAGAATG 
ATTGCCAAGG AAATCCGTCA TATTATTCGA ATGACTTCTG CCAACATGGA CCCTGCCATG 
ATGTTCCGAC AGAGGAAGAA GAGGTGGCGG AGTCTGGGGT CATTGAGTCA AGGCAGCACA 
AATTCTAACA TGCTAGATGT CCAGGGAGGT GCTCACAAAA AAAGAGCACG CCGCAGCTCA 
CTGCTGAATG CCAAGAAACT GTATGAAGAT GCCCAAATGG CAAGGAAAGT GAAACAGTAT 
CTTTCTAGTC TGGACATAGA CACAGATGAG GAGAAGTTCC AGATGATGTC ACTGCAGTGG 
GAACCTGCAT ATGGTACCTT GACAAAGAAC TTAACTGAAA AAAGGTCAGC CAAATCATCT 
GAGATGTCTC CTGTACCATT GAGGTCAGTT GGCCAAACAG CTAAAGTCCA CTTGCATCAG 
CCACACAGAG TGAGCCAGGT GCTTCAGGTG CCAGCAGTTA ACTTGCACCC CATCCGGAAG 
AAAGGACAAG CCAAAGACCA TGTGCTGAGT ACAAGTTTAC CTCAGAAAGG TTTAGGACCA 
ACTGAGGAAG TGAGTGTTAA GAAACACACA GAAGACACTA TTTCTGTAGC GTCCTCTCTG 
CACTCTAGCC CTCCTGCATC TCCTCAGAAC TCCCCACGCA AAGGTTATAC ACTCACACCA 
TCAAGTAAAT GTGACAACTT GTCTGACTCC AGCCACAGTG AAATCTCCTC TCGGTCCAGC 
ATTGTGAGCA ATGGTTCTGT TGACTCCATG TCTGCAGCTG GGCAGGATGA ACGCTGTTCC 
TCCCACAGTC TGGCAGTTCC TGAGCCCACA GGGGCATTGG AAAAGACAGA TCACCCTTCC 
GGGATCTCAG ACCACAGTCA GCTTGCTCAC GGGTGGATGC TGTCAAAGCC GTGTCTTATC 
AAGGGTGTAG CTGTCTCATC TTCTCTGAGC AGCGAAGAGA TGTCTCATGA GCATGTTGTG 
CTGGAAGCAG CTGACAGTGG GCGGGGCAGT TGGACTTCCT GTTCCAGCAG CTCCCATGAC 
AACTTCCAAA GCCTTCAAAA TCAAAAAAGC TGGGACTTCC TGAATTCTTA CAGACACATG 
CATTTAGATG ACCCTATTGC AGAAGTTGAG CCCACTGACT GTGAGCCCTG TGCCTGTCCT 
AAAGGCTGCT CAAGAACTTG TGGCCAGTGC AAAGGCAGCC TAGAGACGAA CCAGCTGAGG 
CAGAGTTGGG CCTCCTCCAG CTCACTCTCG GACACATGTG AACCAAACTA CGGGACAGTT 
AAGCGGAGGG TGTTGGAAAG TGCACCAGCA GAGGCACCTG ATGGCTTGGA GCCCAGGGAT 
ACCACTGACC CTGTTTATAA AACTGTCACT TCCAGTACAG ACAAGGGCTT GATTGTGTAC 
TGTGTCACCT CACCCAAGAA AGGTGATAGG TATAGGGAGC CACCGCCCAC TCCTCCAGGA 
TATCTGGGAA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCCGCACCT AAAACCTCCA 
GACTATAGTG TGGCAGTACA GAGATCAAAG ATGATGCTTA ACAGCCTGTC TAGACTGCCG 
CCAGCTCCTC CCAGTAGCCA CACCTCGGCC TGGGTTCCCT CGAAGATTGG ATCCCAGCCT 
CAAAGGCATA GCCATCCCAA GCTAGCAGAT GTGGCTGATG CAGATAGCGA AGCAGATGAA 
AACGAGCAGG TGTCAGCAGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532628.1
CDS	1467..5870
Translation	MAFLVRCYANCLQPWASKLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532565.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532565.3

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGCT 
GTTGGTTTTT ACTATATTCC TGATCAAGTT ATAAGAGTTT TCAAAGCAGA TCAACAAAGT 
TGCTACATTA TCATCAGTAA GGATACCACA GCTAAAGAAG TGGTCTGCCA AGCTGTACAG 
GAATTCGGTT TGACTGGTGC ATCTGACACA TACTCCCTCT GTGAAGTGTC TGTTACTCCT 
GAGGGTGTTA TAAAGCAGAG AAGACTTCCG GACCAGTTCT CCAAATTAGC TGATAGAATT 
CAACTCAATG GAAGGTATTA CCTAAAAAAC AACATGGAAA CAGAAACATT GTGTTCTGAT 
GAAGATGCTC AGGAACTACT TAAGGAAAGC CAGCTCTCCA TGCTGCAGCT CAGTACCATC 
GAGGTCGCCA CCCAGCTCTC CATGAGAGAC TTTGATTTAT TTCGTAACAT TGAGCCTACT 
GAATACATTG ATGATCTTTT TAAATTAGAT TCCAAAACAG GAAATACTCA CTTGAAACAA 
TTTGAGGATA TTGTAAACCA AGAGACATTC TGGGTTGCCT CAGAGATTTT AAGTGAGTCA 
AATCAGCTGA AAAGAATGAA GATTATTAAA CATTTTATCA AAATTGCTCT TCATTGCCGA 
GAATGCAAGA ATTTCAATTC CATGTTTGCA ATAATAAGTG GCTTGAACCT GGCACCGGTA 
GCACGACTCA GAGGCACTTG GGAAAAGTTG CCAAGCAAAT ATGAGAAACA TCTTCAAGAT 
CTACAAGACC TTTTTGATCC ATCTAGAAAT ATGGCAAAAT ACAGAAATAT TCTTAGTAGT 
CAAAGCATGC AGCCTCCAAT TATTCCACTC TTCCCTGTTG TCAAGAAAGA TATGACATTT 
CTCCATGAAG GAAATGACTC CAAAGTAGAT GGCTTAGTAA ACTTCGAGAA GCTAAGAATG 
ATTGCCAAGG AAATCCGTCA TATTATTCGA ATGACTTCTG CCAACATGGA CCCTGCCATG 
ATGTTCCGAC AGAGGAAGAA GAGGTGGCGG AGTCTGGGGT CATTGAGTCA AGGCAGCACA 
AATTCTAACA TGCTAGATGT CCAGGGAGGT GCTCACAAAA AAAGAGCACG CCGCAGCTCA 
CTGCTGAATG CCAAGAAACT GTATGAAGAT GCCCAAATGG CAAGGAAAGT GAAACAGTAT 
CTTTCTAGTC TGGACATAGA CACAGATGAG GAGAAGTTCC AGATGATGTC ACTGCAGTGG 
GAACCTGCAT ATGGTACCTT GACAAAGAAC TTAACTGAAA AAAGGTCAGC CAAATCATCT 
GAGATGTCTC CTGTACCATT GAGGTCAGTT GGCCAAACAG CTAAAGTCCA CTTGCATCAG 
CCACACAGAG TGAGCCAGGT GCTTCAGGTG CCAGCAGTTA ACTTGCACCC CATCCGGAAG 
AAAGGACAAG CCAAAGACCA TGTGCTGAGT ACAAGTTTAC CTCAGAAAGG TTTAGGACCA 
ACTGAGGAAG TGAGTGTTAA GAAACACACA GAAGACACTA TTTCTGTAGC GTCCTCTCTG 
CACTCTAGCC CTCCTGCATC TCCTCAGAAC TCCCCACGCA AAGGTTATAC ACTCACACCA 
TCAAGTAAAT GTGACAACTT GTCTGACTCC AGCCACAGTG AAATCTCCTC TCGGTCCAGC 
ATTGTGAGCA ATGGTTCTGT TGACTCCATG TCTGCAGCTG GGCAGGATGA ACGCTGTTCC 
TCCCACAGTC TGGCAGTTCC TGAGCCCACA GGGGCATTGG AAAAGACAGA TCACCCTTCC 
GGGATCTCAG ACCACAGTCA GCTTGCTCAC GGGTGGATGC TGTCAAAGCC GTGTCTTATC 
AAGGGTGTAG CTGTCTCATC TTCTCTGAGC AGCGAAGAGA TGTCTCATGA GCATGTTGTG 
CTGGAAGCAG CTGACAGTGG GCGGGGCAGT TGGACTTCCT GTTCCAGCAG CTCCCATGAC 
AACTTCCAAA GCCTTCAAAA TCAAAAAAGC TGGGACTTCC TGAATTCTTA CAGACACATG 
CATTTAGATG ACCCTATTGC AGAAGTTGAG CCCACTGACT GTGAGCCCTG TGCCTGTCCT 
AAAGGCTGCT CAAGAACTTG TGGCCAGTGC AAAGGCAGCC TAGAGACGAA CCAGCTGAGG 
CAGAGTTGGG CCTCCTCCAG CTCACTCTCG GACACATGTG AACCAAACTA CGGGACAGTT 
AAGCGGAGGG TGTTGGAAAG TGCACCAGCA GAGGCACCTG ATGGCTTGGA GCCCAGGGAT 
ACCACTGACC CTGTTTATAA AACTGTCACT TCCAGTACAG ACAAGGGCTT GATTGTGTAC 
TGTGTCACCT CACCCAAGAA AGGTGATAGG TATAGGGAGC CACCGCCCAC TCCTCCAGGA 
TATCTGGGAA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCCGCACCT AAAACCTCCA 
GACTATAGTG TGGCAGTACA GAGATCAAAG ATGATGCTTA ACAGCCTGTC TAGACTGCCG 
CCAGCTCCTC CCAGTAGCCA CACCTCGGCC TGGGTTCCCT CGAAGATTGG ATCCCAGCCT 
CAAAGGCATA GCCATCCCAA GCTAGCAGAT GTGGCTGATG CAGATAGCGA AGCAGATGAA 
AACGAGCAGG TGTCAGCAGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532566.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532629.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA.

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CloneID	OMu45802
Clone ID Related Accession (Same CDS sequence)	XM_006532566.4 , XM_006532566.3
Accession Version	XM_006532566.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532566.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAAC AAAGTTGCTA CATTATCATC 
AGTAAGGATA CCACAGCTAA AGAAGTGGTC TGCCAAGCTG TACAGGAATT CGGTTTGACT 
GGTGCATCTG ACACATACTC CCTCTGTGAA GTGTCTGTTA CTCCTGAGGG TGTTATAAAG 
CAGAGAAGAC TTCCGGACCA GTTCTCCAAA TTAGCTGATA GAATTCAACT CAATGGAAGG 
TATTACCTAA AAAACAACAT GGAAACAGAA ACATTGTGTT CTGATGAAGA TGCTCAGGAA 
CTACTTAAGG AAAGCCAGCT CTCCATGCTG CAGCTCAGTA CCATCGAGGT CGCCACCCAG 
CTCTCCATGA GAGACTTTGA TTTATTTCGT AACATTGAGC CTACTGAATA CATTGATGAT 
CTTTTTAAAT TAGATTCCAA AACAGGAAAT ACTCACTTGA AACAATTTGA GGATATTGTA 
AACCAAGAGA CATTCTGGGT TGCCTCAGAG ATTTTAAGTG AGTCAAATCA GCTGAAAAGA 
ATGAAGATTA TTAAACATTT TATCAAAATT GCTCTTCATT GCCGAGAATG CAAGAATTTC 
AATTCCATGT TTGCAATAAT AAGTGGCTTG AACCTGGCAC CGGTAGCACG ACTCAGAGGC 
ACTTGGGAAA AGTTGCCAAG CAAATATGAG AAACATCTTC AAGATCTACA AGACCTTTTT 
GATCCATCTA GAAATATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTCCC TGTTGTCAAG AAAGATATGA CATTTCTCCA TGAAGGAAAT 
GACTCCAAAG TAGATGGCTT AGTAAACTTC GAGAAGCTAA GAATGATTGC CAAGGAAATC 
CGTCATATTA TTCGAATGAC TTCTGCCAAC ATGGACCCTG CCATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCATTG AGTCAAGGCA GCACAAATTC TAACATGCTA 
GATGTCCAGG GAGGTGCTCA CAAAAAAAGA GCACGCCGCA GCTCACTGCT GAATGCCAAG 
AAACTGTATG AAGATGCCCA AATGGCAAGG AAAGTGAAAC AGTATCTTTC TAGTCTGGAC 
ATAGACACAG ATGAGGAGAA GTTCCAGATG ATGTCACTGC AGTGGGAACC TGCATATGGT 
ACCTTGACAA AGAACTTAAC TGAAAAAAGG TCAGCCAAAT CATCTGAGAT GTCTCCTGTA 
CCATTGAGGT CAGTTGGCCA AACAGCTAAA GTCCACTTGC ATCAGCCACA CAGAGTGAGC 
CAGGTGCTTC AGGTGCCAGC AGTTAACTTG CACCCCATCC GGAAGAAAGG ACAAGCCAAA 
GACCATGTGC TGAGTACAAG TTTACCTCAG AAAGGTTTAG GACCAACTGA GGAAGTGAGT 
GTTAAGAAAC ACACAGAAGA CACTATTTCT GTAGCGTCCT CTCTGCACTC TAGCCCTCCT 
GCATCTCCTC AGAACTCCCC ACGCAAAGGT TATACACTCA CACCATCAAG TAAATGTGAC 
AACTTGTCTG ACTCCAGCCA CAGTGAAATC TCCTCTCGGT CCAGCATTGT GAGCAATGGT 
TCTGTTGACT CCATGTCTGC AGCTGGGCAG GATGAACGCT GTTCCTCCCA CAGTCTGGCA 
GTTCCTGAGC CCACAGGGGC ATTGGAAAAG ACAGATCACC CTTCCGGGAT CTCAGACCAC 
AGTCAGCTTG CTCACGGGTG GATGCTGTCA AAGCCGTGTC TTATCAAGGG TGTAGCTGTC 
TCATCTTCTC TGAGCAGCGA AGAGATGTCT CATGAGCATG TTGTGCTGGA AGCAGCTGAC 
AGTGGGCGGG GCAGTTGGAC TTCCTGTTCC AGCAGCTCCC ATGACAACTT CCAAAGCCTT 
CAAAATCAAA AAAGCTGGGA CTTCCTGAAT TCTTACAGAC ACATGCATTT AGATGACCCT 
ATTGCAGAAG TTGAGCCCAC TGACTGTGAG CCCTGTGCCT GTCCTAAAGG CTGCTCAAGA 
ACTTGTGGCC AGTGCAAAGG CAGCCTAGAG ACGAACCAGC TGAGGCAGAG TTGGGCCTCC 
TCCAGCTCAC TCTCGGACAC ATGTGAACCA AACTACGGGA CAGTTAAGCG GAGGGTGTTG 
GAAAGTGCAC CAGCAGAGGC ACCTGATGGC TTGGAGCCCA GGGATACCAC TGACCCTGTT 
TATAAAACTG TCACTTCCAG TACAGACAAG GGCTTGATTG TGTACTGTGT CACCTCACCC 
AAGAAAGGTG ATAGGTATAG GGAGCCACCG CCCACTCCTC CAGGATATCT GGGAATTTCT 
TTAGCGGACC TAAAGGAAGG ACCCCACCCG CACCTAAAAC CTCCAGACTA TAGTGTGGCA 
GTACAGAGAT CAAAGATGAT GCTTAACAGC CTGTCTAGAC TGCCGCCAGC TCCTCCCAGT 
AGCCACACCT CGGCCTGGGT TCCCTCGAAG ATTGGATCCC AGCCTCAAAG GCATAGCCAT 
CCCAAGCTAG CAGATGTGGC TGATGCAGAT AGCGAAGCAG ATGAAAACGA GCAGGTGTCA 
GCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532629.1
CDS	1466..5854
Translation	MAFLVRCYANCLQPWASKLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGLEPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_006532566.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532566.3

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGATG TTACGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCTGATCTTA CCAAGATGCA CCTCACAGAC AACCCTCATC CACAGGTGAC GCATGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATTGCCAGT GACTCTGGGA GCAGCAGTTT ATCCGATATC 
TATCAGGCTA CAGAGAGTGA AGTAGGAGAT GTAGATTTAA CACGCCTTCC AGAAGGACCT 
GTTGATTCTG AGGATGAAGA GGAGGAGGAA GAAGAGATTG ATAGAACAGA TCCACTGCAG 
GGTCGAGATC TTGTTCGGGA GTGTCTTGAA AAGGAACCCG CAGACAAAAC TGATGATGAT 
GTTGAACAGT TACTTGAGTT CATGCACCAG CTGCCTGCCT TTGCGAACAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC CGTCATGGTT TTTGAAGTAG TAGAGCAGGC TGGGGCTGTT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTCA TTTTAAATGG CACTGTAGAA 
ATTAGTCATC CAGATGGGAA AATAGAAAAT CTCTTCATGG GAAATAGTTT TGGGATTGTC 
CCCACGCTGG ATAAGCAGCA CATGCATGGA GCTGTCAGGA CCAAAGTCGA TGATTGTCAG 
TTTGTCTGCA TAGCTCAACA AGACTATTGG AGAATTTTAA ATCATGTGGA AAAAAATACT 
CACAAAGTTG AGGAAGAGGG AGAGATTGTC ATGGTACATG AGCACCGTGA GCTAGACCGG 
AGCGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTGATAGAAG AACATTCTAT TGTGGACCCA ACATACATAG AAGATTTCCT CTTAACTTAC 
AGGACATTTC TTGAAACTCC TTTGGATGTT GGGATCAAGC TTTTGGAATG GTTTAAGATT 
GACAACCTAA GGGATAAGGT GACACGGATT GTACTCTTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAAG AATTTGAAAG AAATTTGGAA 
GATACAAAAA TGAATGGTCA CCTCAGGTTA TTGAATATTG CCTGTGCTGC AAAGGCTAAG 
TGGAGGCAAG TTGTACTGCA GAAGGCGTCC CGTGAGTCCC CGCTGCACTT CTGCCTTACT 
GGGGGCAGTG AGAAGGGATT TGGAGTCTTT GTTGAAGAAG TAGAATCTGG TAGCAAAGCT 
GCTGACGCAG GACTGAAAAG GGGTGATCAG GTAATGGAAG TCAATGGACA AAACTTTGAG 
AATATTACAC TTGCTAAAGC CCTTGAAATT TTGAGAAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTGCTCAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATTCAAGAT 
GTCCCAGGGG ATATGGAGCA GGCACCACAG GAGAAAGGAA ATAAGAAAAT TAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTATTTAG TGACGGAGGC CTGAGTCAAT CACAAGATGA CAGCATTGTG 
GGAACCAGGC ACTGTAGGCA CAGCCTGGCT ATAATGCCAA TTCCCGGAAC ACTTTCATCC 
AGCAGCCCAG ATCTCTTACA GCCTACCACT AGCATGTTGG ACTTTTCCAA TCCTTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAAC AAAGTTGCTA CATTATCATC 
AGTAAGGATA CCACAGCTAA AGAAGTGGTC TGCCAAGCTG TACAGGAATT CGGTTTGACT 
GGTGCATCTG ACACATACTC CCTCTGTGAA GTGTCTGTTA CTCCTGAGGG TGTTATAAAG 
CAGAGAAGAC TTCCGGACCA GTTCTCCAAA TTAGCTGATA GAATTCAACT CAATGGAAGG 
TATTACCTAA AAAACAACAT GGAAACAGAA ACATTGTGTT CTGATGAAGA TGCTCAGGAA 
CTACTTAAGG AAAGCCAGCT CTCCATGCTG CAGCTCAGTA CCATCGAGGT CGCCACCCAG 
CTCTCCATGA GAGACTTTGA TTTATTTCGT AACATTGAGC CTACTGAATA CATTGATGAT 
CTTTTTAAAT TAGATTCCAA AACAGGAAAT ACTCACTTGA AACAATTTGA GGATATTGTA 
AACCAAGAGA CATTCTGGGT TGCCTCAGAG ATTTTAAGTG AGTCAAATCA GCTGAAAAGA 
ATGAAGATTA TTAAACATTT TATCAAAATT GCTCTTCATT GCCGAGAATG CAAGAATTTC 
AATTCCATGT TTGCAATAAT AAGTGGCTTG AACCTGGCAC CGGTAGCACG ACTCAGAGGC 
ACTTGGGAAA AGTTGCCAAG CAAATATGAG AAACATCTTC AAGATCTACA AGACCTTTTT 
GATCCATCTA GAAATATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTCCC TGTTGTCAAG AAAGATATGA CATTTCTCCA TGAAGGAAAT 
GACTCCAAAG TAGATGGCTT AGTAAACTTC GAGAAGCTAA GAATGATTGC CAAGGAAATC 
CGTCATATTA TTCGAATGAC TTCTGCCAAC ATGGACCCTG CCATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCATTG AGTCAAGGCA GCACAAATTC TAACATGCTA 
GATGTCCAGG GAGGTGCTCA CAAAAAAAGA GCACGCCGCA GCTCACTGCT GAATGCCAAG 
AAACTGTATG AAGATGCCCA AATGGCAAGG AAAGTGAAAC AGTATCTTTC TAGTCTGGAC 
ATAGACACAG ATGAGGAGAA GTTCCAGATG ATGTCACTGC AGTGGGAACC TGCATATGGT 
ACCTTGACAA AGAACTTAAC TGAAAAAAGG TCAGCCAAAT CATCTGAGAT GTCTCCTGTA 
CCATTGAGGT CAGTTGGCCA AACAGCTAAA GTCCACTTGC ATCAGCCACA CAGAGTGAGC 
CAGGTGCTTC AGGTGCCAGC AGTTAACTTG CACCCCATCC GGAAGAAAGG ACAAGCCAAA 
GACCATGTGC TGAGTACAAG TTTACCTCAG AAAGGTTTAG GACCAACTGA GGAAGTGAGT 
GTTAAGAAAC ACACAGAAGA CACTATTTCT GTAGCGTCCT CTCTGCACTC TAGCCCTCCT 
GCATCTCCTC AGAACTCCCC ACGCAAAGGT TATACACTCA CACCATCAAG TAAATGTGAC 
AACTTGTCTG ACTCCAGCCA CAGTGAAATC TCCTCTCGGT CCAGCATTGT GAGCAATGGT 
TCTGTTGACT CCATGTCTGC AGCTGGGCAG GATGAACGCT GTTCCTCCCA CAGTCTGGCA 
GTTCCTGAGC CCACAGGGGC ATTGGAAAAG ACAGATCACC CTTCCGGGAT CTCAGACCAC 
AGTCAGCTTG CTCACGGGTG GATGCTGTCA AAGCCGTGTC TTATCAAGGG TGTAGCTGTC 
TCATCTTCTC TGAGCAGCGA AGAGATGTCT CATGAGCATG TTGTGCTGGA AGCAGCTGAC 
AGTGGGCGGG GCAGTTGGAC TTCCTGTTCC AGCAGCTCCC ATGACAACTT CCAAAGCCTT 
CAAAATCAAA AAAGCTGGGA CTTCCTGAAT TCTTACAGAC ACATGCATTT AGATGACCCT 
ATTGCAGAAG TTGAGCCCAC TGACTGTGAG CCCTGTGCCT GTCCTAAAGG CTGCTCAAGA 
ACTTGTGGCC AGTGCAAAGG CAGCCTAGAG ACGAACCAGC TGAGGCAGAG TTGGGCCTCC 
TCCAGCTCAC TCTCGGACAC ATGTGAACCA AACTACGGGA CAGTTAAGCG GAGGGTGTTG 
GAAAGTGCAC CAGCAGAGGC ACCTGATGGC TTGGAGCCCA GGGATACCAC TGACCCTGTT 
TATAAAACTG TCACTTCCAG TACAGACAAG GGCTTGATTG TGTACTGTGT CACCTCACCC 
AAGAAAGGTG ATAGGTATAG GGAGCCACCG CCCACTCCTC CAGGATATCT GGGAATTTCT 
TTAGCGGACC TAAAGGAAGG ACCCCACCCG CACCTAAAAC CTCCAGACTA TAGTGTGGCA 
GTACAGAGAT CAAAGATGAT GCTTAACAGC CTGTCTAGAC TGCCGCCAGC TCCTCCCAGT 
AGCCACACCT CGGCCTGGGT TCCCTCGAAG ATTGGATCCC AGCCTCAAAG GCATAGCCAT 
CCCAAGCTAG CAGATGTGGC TGATGCAGAT AGCGAAGCAG ATGAAAACGA GCAGGTGTCA 
GCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532567.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532630.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu45803
Clone ID Related Accession (Same CDS sequence)	XM_006532567.4 , XM_006532567.3
Accession Version	XM_006532567.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532567.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGTTGGCA GTATCCTATC TGATCACAGC ATCCTGTTGC GTCTCTCAGG GTCTTCCTCA 
TCCCCTCCCC GTGAGCCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG 
GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACTGGGAGCA CAGCCAAAAG AACAGACAGG 
ACCAAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGGT GTGAGGACAC TACCAGGAGA 
AGGATGACAT CCCCGTTTAG AAGATTTAGG GAGAGAACGC TTTCAAGGGA GAGACTAGTC 
AACAACCAAA AAGAGGATGC ACATCACAAT CAAGCTACAG ACAGCTGTGA GAAAGTGAAG 
GATGTTGGAA GCAACATCAC AGATGAGAGA GGGAATGCCA TCTACGACTC GAGTAGCCAA 
GGCCACAGTA CCGCGCTCAG CTGCTTCTGT ACGCGGTTCA AGACTAAAAG GAGAAAGACT 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532630.1
CDS	4529..8794
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKVGSILSDHSILLRLSGSSSSPPREPSTKISGQSCPGIGGVYLQKKILQITGSTAKRTDRTKKATEENRDRTRCEDTTRRRMTSPFRRFRERTLSRERLVNNQKEDAHHNQATDSCEKVKDVGSNITDERGNAIYDSSSQGHSTALSCFCTRFKTKRRKTL

Target ORF information:

RefSeq Version	XM_006532567.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532567.3

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGC TGTTGGTTTT TACTATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAACAAAG TTGCTACATT ATCATCAGTA AGGATACCAC AGCTAAAGAA 
GTGGTCTGCC AAGCTGTACA GGAATTCGGT TTGACTGGTG CATCTGACAC ATACTCCCTC 
TGTGAAGTGT CTGTTACTCC TGAGGGTGTT ATAAAGCAGA GAAGACTTCC GGACCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACCTAAAAAA CAACATGGAA 
ACAGAAACAT TGTGTTCTGA TGAAGATGCT CAGGAACTAC TTAAGGAAAG CCAGCTCTCC 
ATGCTGCAGC TCAGTACCAT CGAGGTCGCC ACCCAGCTCT CCATGAGAGA CTTTGATTTA 
TTTCGTAACA TTGAGCCTAC TGAATACATT GATGATCTTT TTAAATTAGA TTCCAAAACA 
GGAAATACTC ACTTGAAACA ATTTGAGGAT ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAGATTT TAAGTGAGTC AAATCAGCTG AAAAGAATGA AGATTATTAA ACATTTTATC 
AAAATTGCTC TTCATTGCCG AGAATGCAAG AATTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCACCGGT AGCACGACTC AGAGGCACTT GGGAAAAGTT GCCAAGCAAA 
TATGAGAAAC ATCTTCAAGA TCTACAAGAC CTTTTTGATC CATCTAGAAA TATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTCCATGAA GGAAATGACT CCAAAGTAGA TGGCTTAGTA 
AACTTCGAGA AGCTAAGAAT GATTGCCAAG GAAATCCGTC ATATTATTCG AATGACTTCT 
GCCAACATGG ACCCTGCCAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCATTGAGTC AAGGCAGCAC AAATTCTAAC ATGCTAGATG TCCAGGGAGG TGCTCACAAA 
AAAAGAGCAC GCCGCAGCTC ACTGCTGAAT GCCAAGAAAC TGTATGAAGA TGCCCAAATG 
GCAAGGAAAG TGAAACAGTA TCTTTCTAGT CTGGACATAG ACACAGATGA GGAGAAGTTC 
CAGATGATGT CACTGCAGTG GGAACCTGCA TATGGTACCT TGACAAAGAA CTTAACTGAA 
AAAAGGTCAG CCAAATCATC TGAGATGTCT CCTGTACCAT TGAGGTCAGT TGGCCAAACA 
GCTAAAGTCC ACTTGCATCA GCCACACAGA GTGAGCCAGG TGCTTCAGGT GCCAGCAGTT 
AACTTGCACC CCATCCGGAA GAAAGGACAA GCCAAAGACC ATGTGCTGAG TACAAGTTTA 
CCTCAGAAAG GTTTAGGACC AACTGAGGAA GTGAGTGTTA AGAAACACAC AGAAGACACT 
ATTTCTGTAG CGTCCTCTCT GCACTCTAGC CCTCCTGCAT CTCCTCAGAA CTCCCCACGC 
AAAGTTGGCA GTATCCTATC TGATCACAGC ATCCTGTTGC GTCTCTCAGG GTCTTCCTCA 
TCCCCTCCCC GTGAGCCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG 
GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACTGGGAGCA CAGCCAAAAG AACAGACAGG 
ACCAAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGGT GTGAGGACAC TACCAGGAGA 
AGGATGACAT CCCCGTTTAG AAGATTTAGG GAGAGAACGC TTTCAAGGGA GAGACTAGTC 
AACAACCAAA AAGAGGATGC ACATCACAAT CAAGCTACAG ACAGCTGTGA GAAAGTGAAG 
GATGTTGGAA GCAACATCAC AGATGAGAGA GGGAATGCCA TCTACGACTC GAGTAGCCAA 
GGCCACAGTA CCGCGCTCAG CTGCTTCTGT ACGCGGTTCA AGACTAAAAG GAGAAAGACT 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef6 ( XM_006532568.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532631.1 Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu45804
Clone ID Related Accession (Same CDS sequence)	XM_006532568.4 , XM_006532568.3
Accession Version	XM_006532568.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rap guanine nucleotide exchange factor 6 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532568.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT 
GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA 
CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA 
GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC 
TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA 
TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG 
GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC 
CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT 
AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA 
TCTCCTCAGA ACTCCCCACG CAAAGTTGGC AGTATCCTAT CTGATCACAG CATCCTGTTG 
CGTCTCTCAG GGTCTTCCTC ATCCCCTCCC CGTGAGCCCA GCACCAAGAT CAGTGGCCAG 
AGCTGCCCCG GAATAGGTGG GGTTTACTTG CAAAAGAAAA TCCTTCAGAT TACTGGGAGC 
ACAGCCAAAA GAACAGACAG GACCAAGAAG GCCACTGAGG AGAATAGAGA CAGGACAAGG 
TGTGAGGACA CTACCAGGAG AAGGATGACA TCCCCGTTTA GAAGATTTAG GGAGAGAACG 
CTTTCAAGGG AGAGACTAGT CAACAACCAA AAAGAGGATG CACATCACAA TCAAGCTACA 
GACAGCTGTG AGAAAGTGAA GGATGTTGGA AGCAACATCA CAGATGAGAG AGGGAATGCC 
ATCTACGACT CGAGTAGCCA AGGCCACAGT ACCGCGCTCA GCTGCTTCTG TACGCGGTTC 
AAGACTAAAA GGAGAAAGAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532631.1
CDS	4529..8755
Translation	MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETIARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTLDKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQETFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHIIRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDIDTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKVGSILSDHSILLRLSGSSSSPPREPSTKISGQSCPGIGGVYLQKKILQITGSTAKRTDRTKKATEENRDRTRCEDTTRRRMTSPFRRFRERTLSRERLVNNQKEDAHHNQATDSCEKVKDVGSNITDERGNAIYDSSSQGHSTALSCFCTRFKTKRRKTL

Target ORF information:

RefSeq Version	XM_006532568.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532568.3

ORF Insert Sequence:

ATGAACTCGC CCGTGGACCC CGGCGCTCGG CAGGCACTGA GGAAGAAGCC GCCCGAGCGG 
ACTCCTGAGG ACTTAAATAT TATTTATTCT TATCTGCATG GGATGGAAAT ACTATCAAAC 
CTCAGGGAGC ATCAGCTTAG GTTAATGTCT ACAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAATGC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTACTTG TAAAAGATTC TATGGTCCTT CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTGTTA GAACCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGACAG TATTTTACAA AGAGAAATTC CTGCCAGACA GTCTCGACGA 
AGGTTTCGGA AAATTAACTA TAAAGGAGAA CGCCAAACCA TAATAGATGG AGTGGATATT 
AATAACTACC TTTCGCTTCC AGCTGATCTT ACCAAGATGC ACCTCACAGA CAACCCTCAT 
CCACAGGTGA CGCATGTGTC TTCTAGTCAG TCTGGCTGTA GCATTGCCAG TGACTCTGGG 
AGCAGCAGTT TATCCGATAT CTATCAGGCT ACAGAGAGTG AAGTAGGAGA TGTAGATTTA 
ACACGCCTTC CAGAAGGACC TGTTGATTCT GAGGATGAAG AGGAGGAGGA AGAAGAGATT 
GATAGAACAG ATCCACTGCA GGGTCGAGAT CTTGTTCGGG AGTGTCTTGA AAAGGAACCC 
GCAGACAAAA CTGATGATGA TGTTGAACAG TTACTTGAGT TCATGCACCA GCTGCCTGCC 
TTTGCGAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CCGTCATGGT TTTTGAAGTA 
GTAGAGCAGG CTGGGGCTGT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTC 
ATTTTAAATG GCACTGTAGA AATTAGTCAT CCAGATGGGA AAATAGAAAA TCTCTTCATG 
GGAAATAGTT TTGGGATTGT CCCCACGCTG GATAAGCAGC ACATGCATGG AGCTGTCAGG 
ACCAAAGTCG ATGATTGTCA GTTTGTCTGC ATAGCTCAAC AAGACTATTG GAGAATTTTA 
AATCATGTGG AAAAAAATAC TCACAAAGTT GAGGAAGAGG GAGAGATTGT CATGGTACAT 
GAGCACCGTG AGCTAGACCG GAGCGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTGATAGAA GAACATTCTA TTGTGGACCC AACATACATA 
GAAGATTTCC TCTTAACTTA CAGGACATTT CTTGAAACTC CTTTGGATGT TGGGATCAAG 
CTTTTGGAAT GGTTTAAGAT TGACAACCTA AGGGATAAGG TGACACGGAT TGTACTCTTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAA 
GAATTTGAAA GAAATTTGGA AGATACAAAA ATGAATGGTC ACCTCAGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGGCAA GTTGTACTGC AGAAGGCGTC CCGTGAGTCC 
CCGCTGCACT TCTGCCTTAC TGGGGGCAGT GAGAAGGGAT TTGGAGTCTT TGTTGAAGAA 
GTAGAATCTG GTAGCAAAGC TGCTGACGCA GGACTGAAAA GGGGTGATCA GGTAATGGAA 
GTCAATGGAC AAAACTTTGA GAATATTACA CTTGCTAAAG CCCTTGAAAT TTTGAGAAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTGCTCAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATTCAAGA TGTCCCAGGG GATATGGAGC AGGCACCACA GGAGAAAGGA 
AATAAGAAAA TTAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTATTTA GTGACGGAGG CCTGAGTCAA 
TCACAAGATG ACAGCATTGT GGGAACCAGG CACTGTAGGC ACAGCCTGGC TATAATGCCA 
ATTCCCGGAA CACTTTCATC CAGCAGCCCA GATCTCTTAC AGCCTACCAC TAGCATGTTG 
GACTTTTCCA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAGGAT ACCACAGCTA AAGAAGTGGT CTGCCAAGCT 
GTACAGGAAT TCGGTTTGAC TGGTGCATCT GACACATACT CCCTCTGTGA AGTGTCTGTT 
ACTCCTGAGG GTGTTATAAA GCAGAGAAGA CTTCCGGACC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACCTA AAAAACAACA TGGAAACAGA AACATTGTGT 
TCTGATGAAG ATGCTCAGGA ACTACTTAAG GAAAGCCAGC TCTCCATGCT GCAGCTCAGT 
ACCATCGAGG TCGCCACCCA GCTCTCCATG AGAGACTTTG ATTTATTTCG TAACATTGAG 
CCTACTGAAT ACATTGATGA TCTTTTTAAA TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAACAATTTG AGGATATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA GATTTTAAGT 
GAGTCAAATC AGCTGAAAAG AATGAAGATT ATTAAACATT TTATCAAAAT TGCTCTTCAT 
TGCCGAGAAT GCAAGAATTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
CCGGTAGCAC GACTCAGAGG CACTTGGGAA AAGTTGCCAA GCAAATATGA GAAACATCTT 
CAAGATCTAC AAGACCTTTT TGATCCATCT AGAAATATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTCC ATGAAGGAAA TGACTCCAAA GTAGATGGCT TAGTAAACTT CGAGAAGCTA 
AGAATGATTG CCAAGGAAAT CCGTCATATT ATTCGAATGA CTTCTGCCAA CATGGACCCT 
GCCATGATGT TCCGACAGAG GTCATTGAGT CAAGGCAGCA CAAATTCTAA CATGCTAGAT 
GTCCAGGGAG GTGCTCACAA AAAAAGAGCA CGCCGCAGCT CACTGCTGAA TGCCAAGAAA 
CTGTATGAAG ATGCCCAAAT GGCAAGGAAA GTGAAACAGT ATCTTTCTAG TCTGGACATA 
GACACAGATG AGGAGAAGTT CCAGATGATG TCACTGCAGT GGGAACCTGC ATATGGTACC 
TTGACAAAGA ACTTAACTGA AAAAAGGTCA GCCAAATCAT CTGAGATGTC TCCTGTACCA 
TTGAGGTCAG TTGGCCAAAC AGCTAAAGTC CACTTGCATC AGCCACACAG AGTGAGCCAG 
GTGCTTCAGG TGCCAGCAGT TAACTTGCAC CCCATCCGGA AGAAAGGACA AGCCAAAGAC 
CATGTGCTGA GTACAAGTTT ACCTCAGAAA GGTTTAGGAC CAACTGAGGA AGTGAGTGTT 
AAGAAACACA CAGAAGACAC TATTTCTGTA GCGTCCTCTC TGCACTCTAG CCCTCCTGCA 
TCTCCTCAGA ACTCCCCACG CAAAGTTGGC AGTATCCTAT CTGATCACAG CATCCTGTTG 
CGTCTCTCAG GGTCTTCCTC ATCCCCTCCC CGTGAGCCCA GCACCAAGAT CAGTGGCCAG 
AGCTGCCCCG GAATAGGTGG GGTTTACTTG CAAAAGAAAA TCCTTCAGAT TACTGGGAGC 
ACAGCCAAAA GAACAGACAG GACCAAGAAG GCCACTGAGG AGAATAGAGA CAGGACAAGG 
TGTGAGGACA CTACCAGGAG AAGGATGACA TCCCCGTTTA GAAGATTTAG GGAGAGAACG 
CTTTCAAGGG AGAGACTAGT CAACAACCAA AAAGAGGATG CACATCACAA TCAAGCTACA 
GACAGCTGTG AGAAAGTGAA GGATGTTGGA AGCAACATCA CAGATGAGAG AGGGAATGCC 
ATCTACGACT CGAGTAGCCA AGGCCACAGT ACCGCGCTCA GCTGCTTCTG TACGCGGTTC 
AAGACTAAAA GGAGAAAGAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Misregulation of an Activity-Dependent Splicing Network as a Common Mechanism Underlying Autism Spectrum Disorders.
Molecular cell64(6)1023-1034(2016 Dec)
Quesnel-Valli?res M,Dargaei Z,Irimia M,Gonatopoulos-Pournatzis T,Ip JY,Wu M,Sterne-Weiler T,Nakagawa S,Woodin MA,Blencowe BJ,Cordes SP

Crucial Role of Rapgef2 and Rapgef6, a Family of Guanine Nucleotide Exchange Factors for Rap1 Small GTPase, in Formation of Apical Surface Adherens Junctions and Neural Progenitor Development in the Mouse Cerebral Cortex.
eNeuro3(3)(2016 May-Jun)
Maeta K,Edamatsu H,Nishihara K,Ikutomo J,Bilasy SE,Kataoka T

Deletion of Rapgef6, a candidate schizophrenia susceptibility gene, disrupts amygdala function in mice.
Translational psychiatry5e577(2015 Jun)
Levy RJ,Kvajo M,Li Y,Tsvetkov E,Dong W,Yoshikawa Y,Kataoka T,Bolshakov VY,Karayiorgou M,Gogos JA

Essential roles for the splicing regulator nSR100/SRRM4 during nervous system development.
Genes & development29(7)746-59(2015 Apr)
Quesnel-Valli?res M,Irimia M,Cordes SP,Blencowe BJ

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Rapgef6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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