Abcg4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Abcg4
Entrez Gene ID	192663
Full Name	ATP binding cassette subfamily G member 4
Synonyms	6430517O04Rik
General protein information	Preferred Names ATP binding cassette subfamily G member 4 Names ATP-binding cassette sub-family G member 4 ATP-binding cassette, sub-family G (WHITE), member 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_030244129.1	XP_030099989.1	ATP-binding cassette sub-family G member 4 isoform X2
XM_011242421.3	XP_011240723.1	ATP-binding cassette sub-family G member 4 isoform X1
NM_138955.3	NP_620405.3	ATP-binding cassette sub-family G member 4
XM_011242421.2	XP_011240723.1	ATP-binding cassette sub-family G member 4 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ABCG4	NP_001098813.1
Danio rerio (zebrafish)	abcg4b	NP_001104682.1
Arabidopsis thaliana (thale cress)	ABCG14	NP_564383.1
Rattus norvegicus (Norway rat)	Abcg4	NP_001100286.1
Gallus gallus (chicken)	ABCG4	XP_004948104.1
Danio rerio (zebrafish)	abcg4	XP_687685.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001858	XP_321211.4
Pan troglodytes (chimpanzee)	ABCG4	XP_001165264.1
	LOC100429212	XP_002799910.1
Canis lupus familiaris (dog)	ABCG4	XP_005619736.1
Arabidopsis thaliana (thale cress)	AT3G25620	NP_001189973.1
Arabidopsis thaliana (thale cress)	AT4G27420	NP_194472.3
Homo sapiens (human)	ABCG4	NP_071452.2
Mus musculus (house mouse)	Abcg4	NP_620405.3
Drosophila melanogaster (fruit fly)	Atet	NP_001097078.1
Xenopus tropicalis (tropical clawed frog)	abcg4	XP_004916109.1

Gene Ontology
Bibliography

Related articles in PubMed

Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis.
Murphy AJ, Bijl N, Yvan-Charvet L, Welch CB, Bhagwat N, Reheman A, Wang Y, Shaw JA, Levine RL, Ni H, Tall AR, Wang N
Nature medicine19(5)586-94(2013 May)

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
Tarantino C, Paolella G, Cozzuto L, Minopoli G, Pastore L, Parisi S, Russo T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)

Differential expression and function of ABCG1 and ABCG4 during development and aging.
Bojanic DD, Tarr PT, Gale GD, Smith DJ, Bok D, Chen B, Nusinowitz S, L?vgren-Sandblom A, Bj?rkhem I, Edwards PA
Journal of lipid research51(1)169-81(2010 Jan)

ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain.
Wang N, Yvan-Charvet L, L?tjohann D, Mulder M, Vanmierlo T, Kim TW, Tall AR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology22(4)1073-82(2008 Apr)

ABCG1 and ABCG4 are coexpressed in neurons and astrocytes of the CNS and regulate cholesterol homeostasis through SREBP-2.
Tarr PT, Edwards PA
Journal of lipid research49(1)169-82(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

transplantation of bone marrow deficient in ABCG4, a transporter of unknown function, into Ldlr(-/-) mice resulted in thrombocytosis, accelerated thrombosis and atherosclerosis
Title: Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis.

The loss of ABCG1 and/or ABCG4 from the CNS results in changes in metabolic pathways and in behavior.
Title: Differential expression and function of ABCG1 and ABCG4 during development and aging.

a role for Abcg4 in sterastrocyol efflux in the brain, Abcg4 promotes efflux of cholesterol, desmosterol, and possibly other sterol biosynthetic intermediates to HDL.
Title: ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain.

based on the results, we propose that ABCG1 and ABCG4 mediate the intracellular vesicular transport of cholesterol/sterols within both neurons and astrocytes to regulate cholesterol transport in the brain
Title: ABCG1 and ABCG4 are coexpressed in neurons and astrocytes of the CNS and regulate cholesterol homeostasis through SREBP-2.

ABCG1 and ABCG4 act in concert with ABCA1 to maximize the removal of excess cholesterol from cells and to generate cholesterol-rich lipoprotein particles
Title: ABCA1 and ABCG1 or ABCG4 act sequentially to remove cellular cholesterol and generate cholesterol-rich HDL.

The following Abcg4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Abcg4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20915	NM_138955.3 Latest version!	Mus musculus ATP binding cassette subfamily G member 4 (Abcg4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu90107	XM_030244129.1 Latest version!	Mus musculus ATP binding cassette subfamily G member 4 (Abcg4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu20915	XM_011242421.3 Latest version!	Mus musculus ATP binding cassette subfamily G member 4 (Abcg4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu20915	XM_011242421.2	Mus musculus ATP-binding cassette, sub-family G (WHITE), member 4 (Abcg4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Abcg4 ( NM_138955.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_620405.3 Mus musculus ATP binding cassette subfamily G member 4 (Abcg4), mRNA.

Price & Availability↑

CloneID	OMu20915
Clone ID Related Accession (Same CDS sequence)	XM_011242421.3 , NM_138955.3 , XM_011242421.2
Accession Version	NM_138955.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Mus musculus(house mouse)
Product	ATP-binding cassette sub-family G member 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124577.5. On Apr 11, 2007 this sequence version replaced NM_138955.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK043147.1, AK157317.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGA AGGCATTGGA GGCCGTGGGC TGTGGACTGG GCCCTGGGGC TGTGGCCATG 
GCTGTGACAC TGGAGGACGG GGCAGAGCCC CCTGTGCTGA CCACGCACCT AAAGAAGGTG 
GAGAACCATA TCACAGAAGC ACAGCGCTTC TCTCACCTGC CCAAGCGCTC TGCTGTGGAC 
ATCGAGTTCG TGGAACTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAACGGGGT 
TACAAGACCC TCCTCAAGTG CCTGTCTGGT AAGTTCTGTC GCCGAGAGCT GATTGGCATC 
ATGGGCCCCT CTGGGGCTGG CAAGTCCACA TTCATGAACA TCTTGGCAGG GTACAGGGAG 
TCGGGGATGA AGGGGCAGAT CCTGGTTAAC GGGAGGCCGC GCGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAGGATGAC ATGCTACTGC CTCACCTCAC AGTCCTGGAG 
GCCATGATGG TCTCTGCCAA CCTGAAGCTG AGTGAGAAGC AAGAGGTGAA GAAGGAACTG 
GTGACAGAGA TCCTGACAGC CCTGGGACTG ATGTCCTGCT CTCACACGAG GACAGCTCTG 
CTCTCTGGAG GGCAGAGGAA GCGCCTGGCC ATTGCTCTGG AACTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC TACCAGCGGT CTAGACAGCG CTTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC CCATGGGGGC CGCACCGTCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG GCAATGCATC 
TTCAAGGGCG TGGTTACCAA CCTGATTCCC TATCTGAAGG GGCTTGGCTT GCACTGTCCC 
ACCTACCACA ACCCCGCTGA CTTCATCATT GAGGTGGCCT CTGGAGAGTA TGGAGACCTG 
AACCCCATGC TGTTCAGGGC TGTGCAGAAT GGCCTTTGCA CCATGGCCGA GAAGAAGAGC 
AGCCCTGGGA AGAATGAGCT CCCTGCCCAC TGCCCCACTT GCCCTCCGGA GCTGGATCCT 
ATTGAAAGCC ACACATTTGC CACCAGCACC CTAACCCAGT TCTGCATCCT CTTCAGGAGG 
ACTTTCTTGT CCATCCTCAG GGACACGGTC CTGACCCACT TGCGGTTCAT GTCACATGTG 
TTGATCGGCG TCCTCATTGG CCTCCTCTAC CTGCACATTG GCGATGATGC CAGCAAAGTC 
TTCAACAACA CCGGCTTCCT CTTCTTTTCC ATGCTCTTCC TCATGTTTGC AGCCCTCATG 
CCGACCGTGC TCACCTTTCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CTTGAACTAC 
TGGTATACTC TCAAAGCCTA TTACTTGGCC AAGACCATGG CTGATGTGCC CTTCCAGGTG 
GTATGCCCAG TGGTGTACTG CAGCATCGTG TACTGGATGA CAGGCCAGCC AGCCGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCTTGGCC ATAGCCACCG CCTTAGTTGC CCAGTCTTTG 
GGACTCTTGA TTGGCGCTGC CTCTACCTCC TTGCAGGTGG CCACTTTTGT GGGCCCAGTG 
ACTGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTCGTCA GTTTCAAGAC CATCCCCACT 
TACTTGCAAT GGAGCTCCTA CCTCTCCTAT GTTAGGTATG GCTTTGAGGG TCTGATCCTG 
ACCATCTATG GTATGGAGCG AGGACACCTG ACCTGCTTAG ATGAACAGTG CCCATTCCGG 
GACCCACAAA TCATTTTGCG TGAGCTGGAT GTAGAGGAGG CCAAACTCTA CATGGACTTC 
CTGGTCCTGG GCATTTTCTT CCTTGCCCTG CGGCTGCTGG CATACCTTGT GCTCCGGTAC 
CGGGTCAAGT CTGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620405.3
CDS	244..2184
Translation	MAEKALEAVGCGLGPGAVAMAVTLEDGAEPPVLTTHLKKVENHITEAQRFSHLPKRSAVDIEFVELSYSVREGPCWRKRGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAHGGRTVICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCTMAEKKSSPGKNELPAHCPTCPPELDPIESHTFATSTLTQFCILFRRTFLSILRDTVLTHLRFMSHVLIGVLIGLLYLHIGDDASKVFNNTGFLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYTLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALAIATALVAQSLGLLIGAASTSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGLILTIYGMERGHLTCLDEQCPFRDPQIILRELDVEEAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	NM_138955.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATP binding cassette subfamily G member 4 (Abcg4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138955.3

ORF Insert Sequence:

ATGGCGGAGA AGGCATTGGA GGCCGTGGGC TGTGGACTGG GCCCTGGGGC TGTGGCCATG 
GCTGTGACAC TGGAGGACGG GGCAGAGCCC CCTGTGCTGA CCACGCACCT AAAGAAGGTG 
GAGAACCATA TCACAGAAGC ACAGCGCTTC TCTCACCTGC CCAAGCGCTC TGCTGTGGAC 
ATCGAGTTCG TGGAACTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAACGGGGT 
TACAAGACCC TCCTCAAGTG CCTGTCTGGT AAGTTCTGTC GCCGAGAGCT GATTGGCATC 
ATGGGCCCCT CTGGGGCTGG CAAGTCCACA TTCATGAACA TCTTGGCAGG GTACAGGGAG 
TCGGGGATGA AGGGGCAGAT CCTGGTTAAC GGGAGGCCGC GCGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAGGATGAC ATGCTACTGC CTCACCTCAC AGTCCTGGAG 
GCCATGATGG TCTCTGCCAA CCTGAAGCTG AGTGAGAAGC AAGAGGTGAA GAAGGAACTG 
GTGACAGAGA TCCTGACAGC CCTGGGACTG ATGTCCTGCT CTCACACGAG GACAGCTCTG 
CTCTCTGGAG GGCAGAGGAA GCGCCTGGCC ATTGCTCTGG AACTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC TACCAGCGGT CTAGACAGCG CTTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC CCATGGGGGC CGCACCGTCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG GCAATGCATC 
TTCAAGGGCG TGGTTACCAA CCTGATTCCC TATCTGAAGG GGCTTGGCTT GCACTGTCCC 
ACCTACCACA ACCCCGCTGA CTTCATCATT GAGGTGGCCT CTGGAGAGTA TGGAGACCTG 
AACCCCATGC TGTTCAGGGC TGTGCAGAAT GGCCTTTGCA CCATGGCCGA GAAGAAGAGC 
AGCCCTGGGA AGAATGAGCT CCCTGCCCAC TGCCCCACTT GCCCTCCGGA GCTGGATCCT 
ATTGAAAGCC ACACATTTGC CACCAGCACC CTAACCCAGT TCTGCATCCT CTTCAGGAGG 
ACTTTCTTGT CCATCCTCAG GGACACGGTC CTGACCCACT TGCGGTTCAT GTCACATGTG 
TTGATCGGCG TCCTCATTGG CCTCCTCTAC CTGCACATTG GCGATGATGC CAGCAAAGTC 
TTCAACAACA CCGGCTTCCT CTTCTTTTCC ATGCTCTTCC TCATGTTTGC AGCCCTCATG 
CCGACCGTGC TCACCTTTCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CTTGAACTAC 
TGGTATACTC TCAAAGCCTA TTACTTGGCC AAGACCATGG CTGATGTGCC CTTCCAGGTG 
GTATGCCCAG TGGTGTACTG CAGCATCGTG TACTGGATGA CAGGCCAGCC AGCCGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCTTGGCC ATAGCCACCG CCTTAGTTGC CCAGTCTTTG 
GGACTCTTGA TTGGCGCTGC CTCTACCTCC TTGCAGGTGG CCACTTTTGT GGGCCCAGTG 
ACTGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTCGTCA GTTTCAAGAC CATCCCCACT 
TACTTGCAAT GGAGCTCCTA CCTCTCCTAT GTTAGGTATG GCTTTGAGGG TCTGATCCTG 
ACCATCTATG GTATGGAGCG AGGACACCTG ACCTGCTTAG ATGAACAGTG CCCATTCCGG 
GACCCACAAA TCATTTTGCG TGAGCTGGAT GTAGAGGAGG CCAAACTCTA CATGGACTTC 
CTGGTCCTGG GCATTTTCTT CCTTGCCCTG CGGCTGCTGG CATACCTTGT GCTCCGGTAC 
CGGGTCAAGT CTGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcg4 ( XM_011242421.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240723.1 Mus musculus ATP-binding cassette, sub-family G (WHITE), member 4 (Abcg4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20915
Clone ID Related Accession (Same CDS sequence)	XM_011242421.3 , NM_138955.3 , XM_011242421.2
Accession Version	XM_011242421.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ATP-binding cassette sub-family G member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA AGGCATTGGA GGCCGTGGGC TGTGGACTGG GCCCTGGGGC TGTGGCCATG 
GCTGTGACAC TGGAGGACGG GGCAGAGCCC CCTGTGCTGA CCACGCACCT AAAGAAGGTG 
GAGAACCATA TCACAGAAGC ACAGCGCTTC TCTCACCTGC CCAAGCGCTC TGCTGTGGAC 
ATCGAGTTCG TGGAACTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAACGGGGT 
TACAAGACCC TCCTCAAGTG CCTGTCTGGT AAGTTCTGTC GCCGAGAGCT GATTGGCATC 
ATGGGCCCCT CTGGGGCTGG CAAGTCCACA TTCATGAACA TCTTGGCAGG GTACAGGGAG 
TCGGGGATGA AGGGGCAGAT CCTGGTTAAC GGGAGGCCGC GCGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAGGATGAC ATGCTACTGC CTCACCTCAC AGTCCTGGAG 
GCCATGATGG TCTCTGCCAA CCTGAAGCTG AGTGAGAAGC AAGAGGTGAA GAAGGAACTG 
GTGACAGAGA TCCTGACAGC CCTGGGACTG ATGTCCTGCT CTCACACGAG GACAGCTCTG 
CTCTCTGGAG GGCAGAGGAA GCGCCTGGCC ATTGCTCTGG AACTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC TACCAGCGGT CTAGACAGCG CTTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC CCATGGGGGC CGCACCGTCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG GCAATGCATC 
TTCAAGGGCG TGGTTACCAA CCTGATTCCC TATCTGAAGG GGCTTGGCTT GCACTGTCCC 
ACCTACCACA ACCCCGCTGA CTTCATCATT GAGGTGGCCT CTGGAGAGTA TGGAGACCTG 
AACCCCATGC TGTTCAGGGC TGTGCAGAAT GGCCTTTGCA CCATGGCCGA GAAGAAGAGC 
AGCCCTGGGA AGAATGAGCT CCCTGCCCAC TGCCCCACTT GCCCTCCGGA GCTGGATCCT 
ATTGAAAGCC ACACATTTGC CACCAGCACC CTAACCCAGT TCTGCATCCT CTTCAGGAGG 
ACTTTCTTGT CCATCCTCAG GGACACGGTC CTGACCCACT TGCGGTTCAT GTCACATGTG 
TTGATCGGCG TCCTCATTGG CCTCCTCTAC CTGCACATTG GCGATGATGC CAGCAAAGTC 
TTCAACAACA CCGGCTTCCT CTTCTTTTCC ATGCTCTTCC TCATGTTTGC AGCCCTCATG 
CCGACCGTGC TCACCTTTCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CTTGAACTAC 
TGGTATACTC TCAAAGCCTA TTACTTGGCC AAGACCATGG CTGATGTGCC CTTCCAGGTG 
GTATGCCCAG TGGTGTACTG CAGCATCGTG TACTGGATGA CAGGCCAGCC AGCCGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCTTGGCC ATAGCCACCG CCTTAGTTGC CCAGTCTTTG 
GGACTCTTGA TTGGCGCTGC CTCTACCTCC TTGCAGGTGG CCACTTTTGT GGGCCCAGTG 
ACTGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTCGTCA GTTTCAAGAC CATCCCCACT 
TACTTGCAAT GGAGCTCCTA CCTCTCCTAT GTTAGGTATG GCTTTGAGGG TCTGATCCTG 
ACCATCTATG GTATGGAGCG AGGACACCTG ACCTGCTTAG ATGAACAGTG CCCATTCCGG 
GACCCACAAA TCATTTTGCG TGAGCTGGAT GTAGAGGAGG CCAAACTCTA CATGGACTTC 
CTGGTCCTGG GCATTTTCTT CCTTGCCCTG CGGCTGCTGG CATACCTTGT GCTCCGGTAC 
CGGGTCAAGT CTGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240723.1
CDS	1110..3050
Translation	MAEKALEAVGCGLGPGAVAMAVTLEDGAEPPVLTTHLKKVENHITEAQRFSHLPKRSAVDIEFVELSYSVREGPCWRKRGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAHGGRTVICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCTMAEKKSSPGKNELPAHCPTCPPELDPIESHTFATSTLTQFCILFRRTFLSILRDTVLTHLRFMSHVLIGVLIGLLYLHIGDDASKVFNNTGFLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYTLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALAIATALVAQSLGLLIGAASTSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGLILTIYGMERGHLTCLDEQCPFRDPQIILRELDVEEAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	XM_011242421.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATP-binding cassette, sub-family G (WHITE), member 4 (Abcg4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242421.2

ORF Insert Sequence:

ATGGCGGAGA AGGCATTGGA GGCCGTGGGC TGTGGACTGG GCCCTGGGGC TGTGGCCATG 
GCTGTGACAC TGGAGGACGG GGCAGAGCCC CCTGTGCTGA CCACGCACCT AAAGAAGGTG 
GAGAACCATA TCACAGAAGC ACAGCGCTTC TCTCACCTGC CCAAGCGCTC TGCTGTGGAC 
ATCGAGTTCG TGGAACTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAACGGGGT 
TACAAGACCC TCCTCAAGTG CCTGTCTGGT AAGTTCTGTC GCCGAGAGCT GATTGGCATC 
ATGGGCCCCT CTGGGGCTGG CAAGTCCACA TTCATGAACA TCTTGGCAGG GTACAGGGAG 
TCGGGGATGA AGGGGCAGAT CCTGGTTAAC GGGAGGCCGC GCGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAGGATGAC ATGCTACTGC CTCACCTCAC AGTCCTGGAG 
GCCATGATGG TCTCTGCCAA CCTGAAGCTG AGTGAGAAGC AAGAGGTGAA GAAGGAACTG 
GTGACAGAGA TCCTGACAGC CCTGGGACTG ATGTCCTGCT CTCACACGAG GACAGCTCTG 
CTCTCTGGAG GGCAGAGGAA GCGCCTGGCC ATTGCTCTGG AACTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC TACCAGCGGT CTAGACAGCG CTTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC CCATGGGGGC CGCACCGTCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG GCAATGCATC 
TTCAAGGGCG TGGTTACCAA CCTGATTCCC TATCTGAAGG GGCTTGGCTT GCACTGTCCC 
ACCTACCACA ACCCCGCTGA CTTCATCATT GAGGTGGCCT CTGGAGAGTA TGGAGACCTG 
AACCCCATGC TGTTCAGGGC TGTGCAGAAT GGCCTTTGCA CCATGGCCGA GAAGAAGAGC 
AGCCCTGGGA AGAATGAGCT CCCTGCCCAC TGCCCCACTT GCCCTCCGGA GCTGGATCCT 
ATTGAAAGCC ACACATTTGC CACCAGCACC CTAACCCAGT TCTGCATCCT CTTCAGGAGG 
ACTTTCTTGT CCATCCTCAG GGACACGGTC CTGACCCACT TGCGGTTCAT GTCACATGTG 
TTGATCGGCG TCCTCATTGG CCTCCTCTAC CTGCACATTG GCGATGATGC CAGCAAAGTC 
TTCAACAACA CCGGCTTCCT CTTCTTTTCC ATGCTCTTCC TCATGTTTGC AGCCCTCATG 
CCGACCGTGC TCACCTTTCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CTTGAACTAC 
TGGTATACTC TCAAAGCCTA TTACTTGGCC AAGACCATGG CTGATGTGCC CTTCCAGGTG 
GTATGCCCAG TGGTGTACTG CAGCATCGTG TACTGGATGA CAGGCCAGCC AGCCGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCTTGGCC ATAGCCACCG CCTTAGTTGC CCAGTCTTTG 
GGACTCTTGA TTGGCGCTGC CTCTACCTCC TTGCAGGTGG CCACTTTTGT GGGCCCAGTG 
ACTGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTCGTCA GTTTCAAGAC CATCCCCACT 
TACTTGCAAT GGAGCTCCTA CCTCTCCTAT GTTAGGTATG GCTTTGAGGG TCTGATCCTG 
ACCATCTATG GTATGGAGCG AGGACACCTG ACCTGCTTAG ATGAACAGTG CCCATTCCGG 
GACCCACAAA TCATTTTGCG TGAGCTGGAT GTAGAGGAGG CCAAACTCTA CATGGACTTC 
CTGGTCCTGG GCATTTTCTT CCTTGCCCTG CGGCTGCTGG CATACCTTGT GCTCCGGTAC 
CGGGTCAAGT CTGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcg4 ( XM_030244129.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099989.1 Mus musculus ATP binding cassette subfamily G member 4 (Abcg4), transcript variant X2, mRNA.

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CloneID	OMu90107
Clone ID Related Accession (Same CDS sequence)	XM_030244129.1
Accession Version	XM_030244129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-binding cassette sub-family G member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAAG TTTGTGTCAA GTTGACAAAG ACTAACCAGT TCAGGGGTGG CCAGAGGGGT 
GCCTTCCCAC TAGTTCCATC CCTTTCACAG AAGCCGCCCC GCCTTACTCA CCCCTTCTTA 
GCCATATCCA CCTCACTGGT CCCTGGTGAC CTCTCTCTGG ACAGCGGTCT AGACAGCGCT 
TCTTGTTTCC AAGTGGTGTC CCTCATGAAG TCCCTGGCCC ATGGGGGCCG CACCGTCATC 
TGCACCATCC ACCAGCCCAG TGCCAAGCTC TTTGAGATGT TTGACAAGCT CTACATCCTG 
AGCCAGGGGC AATGCATCTT CAAGGGCGTG GTTACCAACC TGATTCCCTA TCTGAAGGGG 
CTTGGCTTGC ACTGTCCCAC CTACCACAAC CCCGCTGACT TCATCATTGA GGTGGCCTCT 
GGAGAGTATG GAGACCTGAA CCCCATGCTG TTCAGGGCTG TGCAGAATGG CCTTTGCACC 
ATGGCCGAGA AGAAGAGCAG CCCTGGGAAG AATGAGCTCC CTGCCCACTG CCCCACTTGC 
CCTCCGGAGC TGGATCCTAT TGAAAGCCAC ACATTTGCCA CCAGCACCCT AACCCAGTTC 
TGCATCCTCT TCAGGAGGAC TTTCTTGTCC ATCCTCAGGG ACACGGTCCT GACCCACTTG 
CGGTTCATGT CACATGTGTT GATCGGCGTC CTCATTGGCC TCCTCTACCT GCACATTGGC 
GATGATGCCA GCAAAGTCTT CAACAACACC GGCTTCCTCT TCTTTTCCAT GCTCTTCCTC 
ATGTTTGCAG CCCTCATGCC GACCGTGCTC ACCTTTCCCT TAGAGATGGC GGTCTTCATG 
AGGGAGCACT TGAACTACTG GTATACTCTC AAAGCCTATT ACTTGGCCAA GACCATGGCT 
GATGTGCCCT TCCAGGTGGT ATGCCCAGTG GTGTACTGCA GCATCGTGTA CTGGATGACA 
GGCCAGCCAG CCGAGACCAG CCGCTTCCTG CTCTTCTCAG CCTTGGCCAT AGCCACCGCC 
TTAGTTGCCC AGTCTTTGGG ACTCTTGATT GGCGCTGCCT CTACCTCCTT GCAGGTGGCC 
ACTTTTGTGG GCCCAGTGAC TGCCATCCCT GTCCTCTTGT TCTCCGGCTT CTTCGTCAGT 
TTCAAGACCA TCCCCACTTA CTTGCAATGG AGCTCCTACC TCTCCTATGT TAGGTATGGC 
TTTGAGGGTC TGATCCTGAC CATCTATGGT ATGGAGCGAG GACACCTGAC CTGCTTAGAT 
GAACAGTGCC CATTCCGGGA CCCACAAATC ATTTTGCGTG AGCTGGATGT AGAGGAGGCC 
AAACTCTACA TGGACTTCCT GGTCCTGGGC ATTTTCTTCC TTGCCCTGCG GCTGCTGGCA 
TACCTTGTGC TCCGGTACCG GGTCAAGTCT GAGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099989.1
CDS	1854..3272
Translation	MTQVCVKLTKTNQFRGGQRGAFPLVPSLSQKPPRLTHPFLAISTSLVPGDLSLDSGLDSASCFQVVSLMKSLAHGGRTVICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCTMAEKKSSPGKNELPAHCPTCPPELDPIESHTFATSTLTQFCILFRRTFLSILRDTVLTHLRFMSHVLIGVLIGLLYLHIGDDASKVFNNTGFLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYTLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALAIATALVAQSLGLLIGAASTSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGLILTIYGMERGHLTCLDEQCPFRDPQIILRELDVEEAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	XM_030244129.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATP binding cassette subfamily G member 4 (Abcg4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244129.1

ORF Insert Sequence:

ATGACTCAAG TTTGTGTCAA GTTGACAAAG ACTAACCAGT TCAGGGGTGG CCAGAGGGGT 
GCCTTCCCAC TAGTTCCATC CCTTTCACAG AAGCCGCCCC GCCTTACTCA CCCCTTCTTA 
GCCATATCCA CCTCACTGGT CCCTGGTGAC CTCTCTCTGG ACAGCGGTCT AGACAGCGCT 
TCTTGTTTCC AAGTGGTGTC CCTCATGAAG TCCCTGGCCC ATGGGGGCCG CACCGTCATC 
TGCACCATCC ACCAGCCCAG TGCCAAGCTC TTTGAGATGT TTGACAAGCT CTACATCCTG 
AGCCAGGGGC AATGCATCTT CAAGGGCGTG GTTACCAACC TGATTCCCTA TCTGAAGGGG 
CTTGGCTTGC ACTGTCCCAC CTACCACAAC CCCGCTGACT TCATCATTGA GGTGGCCTCT 
GGAGAGTATG GAGACCTGAA CCCCATGCTG TTCAGGGCTG TGCAGAATGG CCTTTGCACC 
ATGGCCGAGA AGAAGAGCAG CCCTGGGAAG AATGAGCTCC CTGCCCACTG CCCCACTTGC 
CCTCCGGAGC TGGATCCTAT TGAAAGCCAC ACATTTGCCA CCAGCACCCT AACCCAGTTC 
TGCATCCTCT TCAGGAGGAC TTTCTTGTCC ATCCTCAGGG ACACGGTCCT GACCCACTTG 
CGGTTCATGT CACATGTGTT GATCGGCGTC CTCATTGGCC TCCTCTACCT GCACATTGGC 
GATGATGCCA GCAAAGTCTT CAACAACACC GGCTTCCTCT TCTTTTCCAT GCTCTTCCTC 
ATGTTTGCAG CCCTCATGCC GACCGTGCTC ACCTTTCCCT TAGAGATGGC GGTCTTCATG 
AGGGAGCACT TGAACTACTG GTATACTCTC AAAGCCTATT ACTTGGCCAA GACCATGGCT 
GATGTGCCCT TCCAGGTGGT ATGCCCAGTG GTGTACTGCA GCATCGTGTA CTGGATGACA 
GGCCAGCCAG CCGAGACCAG CCGCTTCCTG CTCTTCTCAG CCTTGGCCAT AGCCACCGCC 
TTAGTTGCCC AGTCTTTGGG ACTCTTGATT GGCGCTGCCT CTACCTCCTT GCAGGTGGCC 
ACTTTTGTGG GCCCAGTGAC TGCCATCCCT GTCCTCTTGT TCTCCGGCTT CTTCGTCAGT 
TTCAAGACCA TCCCCACTTA CTTGCAATGG AGCTCCTACC TCTCCTATGT TAGGTATGGC 
TTTGAGGGTC TGATCCTGAC CATCTATGGT ATGGAGCGAG GACACCTGAC CTGCTTAGAT 
GAACAGTGCC CATTCCGGGA CCCACAAATC ATTTTGCGTG AGCTGGATGT AGAGGAGGCC 
AAACTCTACA TGGACTTCCT GGTCCTGGGC ATTTTCTTCC TTGCCCTGCG GCTGCTGGCA 
TACCTTGTGC TCCGGTACCG GGTCAAGTCT GAGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcg4 ( XM_011242421.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240723.1 Mus musculus ATP binding cassette subfamily G member 4 (Abcg4), transcript variant X1, mRNA.

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CloneID	OMu20915
Clone ID Related Accession (Same CDS sequence)	XM_011242421.3 , NM_138955.3 , XM_011242421.2
Accession Version	XM_011242421.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-binding cassette sub-family G member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA AGGCATTGGA GGCCGTGGGC TGTGGACTGG GCCCTGGGGC TGTGGCCATG 
GCTGTGACAC TGGAGGACGG GGCAGAGCCC CCTGTGCTGA CCACGCACCT AAAGAAGGTG 
GAGAACCATA TCACAGAAGC ACAGCGCTTC TCTCACCTGC CCAAGCGCTC TGCTGTGGAC 
ATCGAGTTCG TGGAACTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAACGGGGT 
TACAAGACCC TCCTCAAGTG CCTGTCTGGT AAGTTCTGTC GCCGAGAGCT GATTGGCATC 
ATGGGCCCCT CTGGGGCTGG CAAGTCCACA TTCATGAACA TCTTGGCAGG GTACAGGGAG 
TCGGGGATGA AGGGGCAGAT CCTGGTTAAC GGGAGGCCGC GCGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAGGATGAC ATGCTACTGC CTCACCTCAC AGTCCTGGAG 
GCCATGATGG TCTCTGCCAA CCTGAAGCTG AGTGAGAAGC AAGAGGTGAA GAAGGAACTG 
GTGACAGAGA TCCTGACAGC CCTGGGACTG ATGTCCTGCT CTCACACGAG GACAGCTCTG 
CTCTCTGGAG GGCAGAGGAA GCGCCTGGCC ATTGCTCTGG AACTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC TACCAGCGGT CTAGACAGCG CTTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC CCATGGGGGC CGCACCGTCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG GCAATGCATC 
TTCAAGGGCG TGGTTACCAA CCTGATTCCC TATCTGAAGG GGCTTGGCTT GCACTGTCCC 
ACCTACCACA ACCCCGCTGA CTTCATCATT GAGGTGGCCT CTGGAGAGTA TGGAGACCTG 
AACCCCATGC TGTTCAGGGC TGTGCAGAAT GGCCTTTGCA CCATGGCCGA GAAGAAGAGC 
AGCCCTGGGA AGAATGAGCT CCCTGCCCAC TGCCCCACTT GCCCTCCGGA GCTGGATCCT 
ATTGAAAGCC ACACATTTGC CACCAGCACC CTAACCCAGT TCTGCATCCT CTTCAGGAGG 
ACTTTCTTGT CCATCCTCAG GGACACGGTC CTGACCCACT TGCGGTTCAT GTCACATGTG 
TTGATCGGCG TCCTCATTGG CCTCCTCTAC CTGCACATTG GCGATGATGC CAGCAAAGTC 
TTCAACAACA CCGGCTTCCT CTTCTTTTCC ATGCTCTTCC TCATGTTTGC AGCCCTCATG 
CCGACCGTGC TCACCTTTCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CTTGAACTAC 
TGGTATACTC TCAAAGCCTA TTACTTGGCC AAGACCATGG CTGATGTGCC CTTCCAGGTG 
GTATGCCCAG TGGTGTACTG CAGCATCGTG TACTGGATGA CAGGCCAGCC AGCCGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCTTGGCC ATAGCCACCG CCTTAGTTGC CCAGTCTTTG 
GGACTCTTGA TTGGCGCTGC CTCTACCTCC TTGCAGGTGG CCACTTTTGT GGGCCCAGTG 
ACTGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTCGTCA GTTTCAAGAC CATCCCCACT 
TACTTGCAAT GGAGCTCCTA CCTCTCCTAT GTTAGGTATG GCTTTGAGGG TCTGATCCTG 
ACCATCTATG GTATGGAGCG AGGACACCTG ACCTGCTTAG ATGAACAGTG CCCATTCCGG 
GACCCACAAA TCATTTTGCG TGAGCTGGAT GTAGAGGAGG CCAAACTCTA CATGGACTTC 
CTGGTCCTGG GCATTTTCTT CCTTGCCCTG CGGCTGCTGG CATACCTTGT GCTCCGGTAC 
CGGGTCAAGT CTGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240723.1
CDS	664..2604
Translation	MAEKALEAVGCGLGPGAVAMAVTLEDGAEPPVLTTHLKKVENHITEAQRFSHLPKRSAVDIEFVELSYSVREGPCWRKRGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAHGGRTVICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCTMAEKKSSPGKNELPAHCPTCPPELDPIESHTFATSTLTQFCILFRRTFLSILRDTVLTHLRFMSHVLIGVLIGLLYLHIGDDASKVFNNTGFLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYTLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALAIATALVAQSLGLLIGAASTSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGLILTIYGMERGHLTCLDEQCPFRDPQIILRELDVEEAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	XM_011242421.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATP binding cassette subfamily G member 4 (Abcg4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242421.3

ORF Insert Sequence:

ATGGCGGAGA AGGCATTGGA GGCCGTGGGC TGTGGACTGG GCCCTGGGGC TGTGGCCATG 
GCTGTGACAC TGGAGGACGG GGCAGAGCCC CCTGTGCTGA CCACGCACCT AAAGAAGGTG 
GAGAACCATA TCACAGAAGC ACAGCGCTTC TCTCACCTGC CCAAGCGCTC TGCTGTGGAC 
ATCGAGTTCG TGGAACTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAACGGGGT 
TACAAGACCC TCCTCAAGTG CCTGTCTGGT AAGTTCTGTC GCCGAGAGCT GATTGGCATC 
ATGGGCCCCT CTGGGGCTGG CAAGTCCACA TTCATGAACA TCTTGGCAGG GTACAGGGAG 
TCGGGGATGA AGGGGCAGAT CCTGGTTAAC GGGAGGCCGC GCGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAGGATGAC ATGCTACTGC CTCACCTCAC AGTCCTGGAG 
GCCATGATGG TCTCTGCCAA CCTGAAGCTG AGTGAGAAGC AAGAGGTGAA GAAGGAACTG 
GTGACAGAGA TCCTGACAGC CCTGGGACTG ATGTCCTGCT CTCACACGAG GACAGCTCTG 
CTCTCTGGAG GGCAGAGGAA GCGCCTGGCC ATTGCTCTGG AACTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC TACCAGCGGT CTAGACAGCG CTTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC CCATGGGGGC CGCACCGTCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG GCAATGCATC 
TTCAAGGGCG TGGTTACCAA CCTGATTCCC TATCTGAAGG GGCTTGGCTT GCACTGTCCC 
ACCTACCACA ACCCCGCTGA CTTCATCATT GAGGTGGCCT CTGGAGAGTA TGGAGACCTG 
AACCCCATGC TGTTCAGGGC TGTGCAGAAT GGCCTTTGCA CCATGGCCGA GAAGAAGAGC 
AGCCCTGGGA AGAATGAGCT CCCTGCCCAC TGCCCCACTT GCCCTCCGGA GCTGGATCCT 
ATTGAAAGCC ACACATTTGC CACCAGCACC CTAACCCAGT TCTGCATCCT CTTCAGGAGG 
ACTTTCTTGT CCATCCTCAG GGACACGGTC CTGACCCACT TGCGGTTCAT GTCACATGTG 
TTGATCGGCG TCCTCATTGG CCTCCTCTAC CTGCACATTG GCGATGATGC CAGCAAAGTC 
TTCAACAACA CCGGCTTCCT CTTCTTTTCC ATGCTCTTCC TCATGTTTGC AGCCCTCATG 
CCGACCGTGC TCACCTTTCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CTTGAACTAC 
TGGTATACTC TCAAAGCCTA TTACTTGGCC AAGACCATGG CTGATGTGCC CTTCCAGGTG 
GTATGCCCAG TGGTGTACTG CAGCATCGTG TACTGGATGA CAGGCCAGCC AGCCGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCTTGGCC ATAGCCACCG CCTTAGTTGC CCAGTCTTTG 
GGACTCTTGA TTGGCGCTGC CTCTACCTCC TTGCAGGTGG CCACTTTTGT GGGCCCAGTG 
ACTGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTCGTCA GTTTCAAGAC CATCCCCACT 
TACTTGCAAT GGAGCTCCTA CCTCTCCTAT GTTAGGTATG GCTTTGAGGG TCTGATCCTG 
ACCATCTATG GTATGGAGCG AGGACACCTG ACCTGCTTAG ATGAACAGTG CCCATTCCGG 
GACCCACAAA TCATTTTGCG TGAGCTGGAT GTAGAGGAGG CCAAACTCTA CATGGACTTC 
CTGGTCCTGG GCATTTTCTT CCTTGCCCTG CGGCTGCTGG CATACCTTGT GCTCCGGTAC 
CGGGTCAAGT CTGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis.
Nature medicine19(5)586-94(2013 May)
Murphy AJ,Bijl N,Yvan-Charvet L,Welch CB,Bhagwat N,Reheman A,Wang Y,Shaw JA,Levine RL,Ni H,Tall AR,Wang N

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)
Tarantino C,Paolella G,Cozzuto L,Minopoli G,Pastore L,Parisi S,Russo T

Differential expression and function of ABCG1 and ABCG4 during development and aging.
Journal of lipid research51(1)169-81(2010 Jan)
Bojanic DD,Tarr PT,Gale GD,Smith DJ,Bok D,Chen B,Nusinowitz S,L?vgren-Sandblom A,Bj?rkhem I,Edwards PA

ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology22(4)1073-82(2008 Apr)
Wang N,Yvan-Charvet L,L?tjohann D,Mulder M,Vanmierlo T,Kim TW,Tall AR

ABCG1 and ABCG4 are coexpressed in neurons and astrocytes of the CNS and regulate cholesterol homeostasis through SREBP-2.
Journal of lipid research49(1)169-82(2008 Jan)
Tarr PT,Edwards PA

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Abcg4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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