Prom2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prom2
Entrez Gene ID	192212
Full Name	prominin 2
Synonyms	AI507243,Prom-rp
General protein information	Preferred Names prominin 2 Names prominin-2 PROM-2 mPROML2 prominin-like protein 2 prominin-related protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_138750.2	NP_620089.2	prominin-2 isoform 1 precursor
NM_178047.4	NP_835148.2	prominin-2 isoform 2 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PROM2	XP_425759.4
Homo sapiens (human)	PROM2	NP_653308.2
Rattus norvegicus (Norway rat)	Prom2	NP_620212.1
Canis lupus familiaris (dog)	PROM2	XP_005630460.1
Bos taurus (cattle)	PROM2	XP_005212435.1
Mus musculus (house mouse)	Prom2	NP_620089.2
Xenopus tropicalis (tropical clawed frog)	prom2	NP_001243221.1
Pan troglodytes (chimpanzee)	PROM2	XP_003309171.1
	PROM2	XP_001095857.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

A Grhl2-dependent gene network controls trophoblast branching morphogenesis.
Walentin K, Hinze C, Werth M, Haase N, Varma S, Morell R, Aue A, P?tschke E, Warburton D, Qiu A, Barasch J, Purf?rst B, Dieterich C, Popova E, Bader M, Dechend R, Staff AC, Yurtdas ZY, Kilic E, Schmidt-Ott KM
Development (Cambridge, England)142(6)1125-36(2015 Mar)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Chromatin regulation by BAF170 controls cerebral cortical size and thickness.
Tuoc TC, Boretius S, Sansom SN, Pitulescu ME, Frahm J, Livesey FJ, Stoykova A
Developmental cell25(3)256-69(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show the distribution and subcellular localization of prominin-2 within the kidney in situ.
Title: Prominin-2 is a novel marker of distal tubules and collecting ducts of the human and murine kidney.

Prominin-2 is widely and abundantly expressed along the epithelia of various segments of the adult male genitourinary tract.
Title: Robust expression of Prominin-2 all along the adult male reproductive system and urinary bladder.

expression Prominin-2, in the three pairs of major salivary glands, i.e., submandibular-, major sublingual-, and parotid glands in adult mice.
Title: Differential expression of Prominin-1 (CD133) and Prominin-2 in major cephalic exocrine glands of adult mice.

These data show that all the characteristics of prominin-1 are shared by prominin-2, which is in agreement with a possible redundancy in their role as potential organizers of plasma membrane protrusions.
Title: Prominin-2 is a cholesterol-binding protein associated with apical and basolateral plasmalemmal protrusions in polarized epithelial cells and released into urine.

identification as well as molecular and cell biological characterization
Title: Characterization of prominin-2, a new member of the prominin family of pentaspan membrane glycoproteins.

The following Prom2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prom2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05145	NM_178047.4 Latest version!	Mus musculus prominin 2 (Prom2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10894	NM_138750.2 Latest version!	Mus musculus prominin 2 (Prom2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$489.30 $699.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prom2 ( NM_178047.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_835148.2 Mus musculus prominin 2 (Prom2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05145
Clone ID Related Accession (Same CDS sequence)	NM_178047.4
Accession Version	NM_178047.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	prominin-2 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ045574.1, AK137749.1, BQ900846.1 and AK029851.1. On Mar 14, 2009 this sequence version replaced NM_178047.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039212.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGCGCT CACCCGGCCT CATGGTGCCC CTGCTGGGCC TGAGCCTGGG ACTGGCCCTG 
AGCCTGCCCG AAGCTGTGAC AGACGACTGC GGGTCCCTCG GGCGCGTGGA GCACCTGGCA 
TTCGCTCGGG TGCCCAGGAC ACGAGAGCTG GCCCCTCTTG TTCGCGCGTC GGGGCCCCTG 
AACTCCCTCT ATGGCACCGT GCGACGATTC CTCTCCGTGG TTCAACTTAA CCCCTTCCCG 
GCAGAGTTGA TAAAGACCCT GCTGAATGAC CCATCTTCTG TGAAGACAGA TGAGGTGGTT 
CGATATGAGG CAGGCTACGT GGTGTGTGCT GTGATTGCTG GCCTCTACCT CCTATTGGTG 
CCCATCACTG GGCTCTGCTT CTGTTGCTGT CGCTGCCGCC AGCGCTGTGG GGGCAGAGTG 
AAGACGGAGC ACAAGGCCAT GGCCTGTGAA CGTGGTACCC TCATGATCTT CCTGCTGCTG 
ACCACCCTCG TGCTTCTGAT TGGTATGGTC TGTGCTTTTG CTACAAACCA ACACACACAT 
AGTCAGACGG GGCCCAGTGT GAAAGCTGTC CCTGAGACCC TGCTCAGCCT AAGAGGCCTG 
GTCTCTGATG TCCCTGAAGA GCTGCGGGCC ATTGCTGAGC AGTTCTCTGT GCCTCAGAAG 
CAAGTCTCAA AGGAGCTGGA TGGTGTTGGT GAGAACCTTG GGAACGTAAT TCATAACCGG 
CTCAAGAGCA CAGTGTACCC AGTGCTAGCC TCAGTGCACA GCCTGGGCCA GGCCCTGCAG 
GTCTCCATAG ACCACCTTCG AGCCCTGAAC ACCACCTCAG TGGAGCTGCA GGAGGCTCAG 
CGGCACCTGG AGCCACCTGT GCAGGCGCAC CGGGAACGCC TGCTCGCCCT GCTTCAGGAC 
AGCTGGTGCC ACGAGGAGAA TTGCAAGCGG GTGCTCAGCC AGGCCGGTGC TCTGCAACTG 
GGCGCTGACT TTAGCCAGAC GCCCCCTGTG GATGATGTCC TGCATCGGCT GAAGGATGTC 
CCAGAAACCA ACTTCTCCAG CATGGTCCAG GAGGAGAAAG CCACCTTCAA TAACCTCCCG 
CTCCTGGTTC AAGTGCAGGC GGTCAGTGTG GTCAAAGATG TGAAAAAGGC ACTGGCTGAG 
CAGCCTGAAG GGTTGAGGAT GCTGGCCCAA GCGTTCCCAG GTTCAGAGGC AGCCTCCCGC 
TGGAGCCAGG CACTGGAGGG GCTGGAACAG CGCAGCCGCC CCTACTTGCA GGAGGTGCAA 
CAATATGAGA CATACAGGTG GATTTTGGGA TGCGTGCTGT GCTCTGCCAT CCTGCTTGTG 
GTGATCTGTA ACCTGCTGGG CCTCAGCCTG GGCATCTGGG GGTTGTTTGC CAGGGAGGAC 
CCCAGCCACT CTGAAACCAA GGGCGAGGCT GGAGCCCGCC TCCTCATGGC AGGTGTGGCC 
TTCAGCTTCC TCTTCGCAGT CCCCCTCATC CTCCTCGTCT TCGTCACTTT CCTGGTGGGT 
GGCAATGTGC AGACGCTGGT GTGCCGGAGC TGGGAGAGTG GAGAGCTGTA TGAGTTTGCA 
GACACTCCGG GGAACTTGCC ACCATCCATG AACCTATCCT ATCTTCTTGG CCTGAAGAAA 
AACATCAGCA TCGTCCAGGC CTATCGACAG TGTAAGGCAG GGGCAGTACT CTGGAAGGTT 
CTACAGCTCA ATGACTCGTA TGACCTGGAT AAGCACCTGG ATATCAAACA GTATACCCAC 
AAGATTCAGC AGGAGCTTCA GAGCTTCCAA GTAGATTTGA AGGAGCTAGA CCTGTTGAGC 
CCCACTGCCC GCCAGGACCT GGAGGCTTTG CAGAGGAGCG GGCTGGAGAA AATCCACTAC 
AGAGGCTTCC TTGTGCAGAT CCAGAAGCCT GTGGTGAACA CTGACATGTG GCAGCTGGCG 
CAGGAGCTGG AAGGACTGGC CCAGGCCCAA AATGACTCTT TACTGAGGCA GCAGCTACGG 
GAGGAGGCCA GAGAACTTCG AAGCCTCTAC CAGGAGAAGG TTGTCCCCCA GGAGAGCCTT 
GTGACCAAGC TCAACTTCAG TGTCAAGACC TTGGAGTCCT TGGCCCCAAG TCTGCAGGTG 
AATACCTCAG ACTTTCTTGA CAGTGTCACT CGTCTAAAAG GAGAGCTGCC TGTGCAGATC 
AACCACATCC TGAGGAATGC CACTGAGTGT TTCCTGACCA GGGAAATGGG CTACTTCTCT 
CAGTATGTGA CCTGGGTGAG AGCAGAGGTG ACTCAGCGCA TCGCCACCTG CCAGCCCTTC 
TCTACAGCCC TGGACAATGG TCATGTGATC CTGTGTGACA TGATGGCTGA CCCCTGGAAC 
GCCTTCTGGT TTTGCCTGGG GTGGTGCACC TTCTTCCTCA TCCCCAGCAT CATCTTCGCT 
GTCAAGACCT CCAAGTACTT CCGTCCCATC CGGAAACGCC TCAGGGTCAC CTCTCTGAAG 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835148.2
CDS	98..2563
Translation	MTRSPGLMVPLLGLSLGLALSLPEAVTDDCGSLGRVEHLAFARVPRTRELAPLVRASGPLNSLYGTVRRFLSVVQLNPFPAELIKTLLNDPSSVKTDEVVRYEAGYVVCAVIAGLYLLLVPITGLCFCCCRCRQRCGGRVKTEHKAMACERGTLMIFLLLTTLVLLIGMVCAFATNQHTHSQTGPSVKAVPETLLSLRGLVSDVPEELRAIAEQFSVPQKQVSKELDGVGENLGNVIHNRLKSTVYPVLASVHSLGQALQVSIDHLRALNTTSVELQEAQRHLEPPVQAHRERLLALLQDSWCHEENCKRVLSQAGALQLGADFSQTPPVDDVLHRLKDVPETNFSSMVQEEKATFNNLPLLVQVQAVSVVKDVKKALAEQPEGLRMLAQAFPGSEAASRWSQALEGLEQRSRPYLQEVQQYETYRWILGCVLCSAILLVVICNLLGLSLGIWGLFAREDPSHSETKGEAGARLLMAGVAFSFLFAVPLILLVFVTFLVGGNVQTLVCRSWESGELYEFADTPGNLPPSMNLSYLLGLKKNISIVQAYRQCKAGAVLWKVLQLNDSYDLDKHLDIKQYTHKIQQELQSFQVDLKELDLLSPTARQDLEALQRSGLEKIHYRGFLVQIQKPVVNTDMWQLAQELEGLAQAQNDSLLRQQLREEARELRSLYQEKVVPQESLVTKLNFSVKTLESLAPSLQVNTSDFLDSVTRLKGELPVQINHILRNATECFLTREMGYFSQYVTWVRAEVTQRIATCQPFSTALDNGHVILCDMMADPWNAFWFCLGWCTFFLIPSIIFAVKTSKYFRPIRKRLRVTSLKL

Target ORF information:

RefSeq Version	NM_178047.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus prominin 2 (Prom2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178047.4

ORF Insert Sequence:

ATGACGCGCT CACCCGGCCT CATGGTGCCC CTGCTGGGCC TGAGCCTGGG ACTGGCCCTG 
AGCCTGCCCG AAGCTGTGAC AGACGACTGC GGGTCCCTCG GGCGCGTGGA GCACCTGGCA 
TTCGCTCGGG TGCCCAGGAC ACGAGAGCTG GCCCCTCTTG TTCGCGCGTC GGGGCCCCTG 
AACTCCCTCT ATGGCACCGT GCGACGATTC CTCTCCGTGG TTCAACTTAA CCCCTTCCCG 
GCAGAGTTGA TAAAGACCCT GCTGAATGAC CCATCTTCTG TGAAGACAGA TGAGGTGGTT 
CGATATGAGG CAGGCTACGT GGTGTGTGCT GTGATTGCTG GCCTCTACCT CCTATTGGTG 
CCCATCACTG GGCTCTGCTT CTGTTGCTGT CGCTGCCGCC AGCGCTGTGG GGGCAGAGTG 
AAGACGGAGC ACAAGGCCAT GGCCTGTGAA CGTGGTACCC TCATGATCTT CCTGCTGCTG 
ACCACCCTCG TGCTTCTGAT TGGTATGGTC TGTGCTTTTG CTACAAACCA ACACACACAT 
AGTCAGACGG GGCCCAGTGT GAAAGCTGTC CCTGAGACCC TGCTCAGCCT AAGAGGCCTG 
GTCTCTGATG TCCCTGAAGA GCTGCGGGCC ATTGCTGAGC AGTTCTCTGT GCCTCAGAAG 
CAAGTCTCAA AGGAGCTGGA TGGTGTTGGT GAGAACCTTG GGAACGTAAT TCATAACCGG 
CTCAAGAGCA CAGTGTACCC AGTGCTAGCC TCAGTGCACA GCCTGGGCCA GGCCCTGCAG 
GTCTCCATAG ACCACCTTCG AGCCCTGAAC ACCACCTCAG TGGAGCTGCA GGAGGCTCAG 
CGGCACCTGG AGCCACCTGT GCAGGCGCAC CGGGAACGCC TGCTCGCCCT GCTTCAGGAC 
AGCTGGTGCC ACGAGGAGAA TTGCAAGCGG GTGCTCAGCC AGGCCGGTGC TCTGCAACTG 
GGCGCTGACT TTAGCCAGAC GCCCCCTGTG GATGATGTCC TGCATCGGCT GAAGGATGTC 
CCAGAAACCA ACTTCTCCAG CATGGTCCAG GAGGAGAAAG CCACCTTCAA TAACCTCCCG 
CTCCTGGTTC AAGTGCAGGC GGTCAGTGTG GTCAAAGATG TGAAAAAGGC ACTGGCTGAG 
CAGCCTGAAG GGTTGAGGAT GCTGGCCCAA GCGTTCCCAG GTTCAGAGGC AGCCTCCCGC 
TGGAGCCAGG CACTGGAGGG GCTGGAACAG CGCAGCCGCC CCTACTTGCA GGAGGTGCAA 
CAATATGAGA CATACAGGTG GATTTTGGGA TGCGTGCTGT GCTCTGCCAT CCTGCTTGTG 
GTGATCTGTA ACCTGCTGGG CCTCAGCCTG GGCATCTGGG GGTTGTTTGC CAGGGAGGAC 
CCCAGCCACT CTGAAACCAA GGGCGAGGCT GGAGCCCGCC TCCTCATGGC AGGTGTGGCC 
TTCAGCTTCC TCTTCGCAGT CCCCCTCATC CTCCTCGTCT TCGTCACTTT CCTGGTGGGT 
GGCAATGTGC AGACGCTGGT GTGCCGGAGC TGGGAGAGTG GAGAGCTGTA TGAGTTTGCA 
GACACTCCGG GGAACTTGCC ACCATCCATG AACCTATCCT ATCTTCTTGG CCTGAAGAAA 
AACATCAGCA TCGTCCAGGC CTATCGACAG TGTAAGGCAG GGGCAGTACT CTGGAAGGTT 
CTACAGCTCA ATGACTCGTA TGACCTGGAT AAGCACCTGG ATATCAAACA GTATACCCAC 
AAGATTCAGC AGGAGCTTCA GAGCTTCCAA GTAGATTTGA AGGAGCTAGA CCTGTTGAGC 
CCCACTGCCC GCCAGGACCT GGAGGCTTTG CAGAGGAGCG GGCTGGAGAA AATCCACTAC 
AGAGGCTTCC TTGTGCAGAT CCAGAAGCCT GTGGTGAACA CTGACATGTG GCAGCTGGCG 
CAGGAGCTGG AAGGACTGGC CCAGGCCCAA AATGACTCTT TACTGAGGCA GCAGCTACGG 
GAGGAGGCCA GAGAACTTCG AAGCCTCTAC CAGGAGAAGG TTGTCCCCCA GGAGAGCCTT 
GTGACCAAGC TCAACTTCAG TGTCAAGACC TTGGAGTCCT TGGCCCCAAG TCTGCAGGTG 
AATACCTCAG ACTTTCTTGA CAGTGTCACT CGTCTAAAAG GAGAGCTGCC TGTGCAGATC 
AACCACATCC TGAGGAATGC CACTGAGTGT TTCCTGACCA GGGAAATGGG CTACTTCTCT 
CAGTATGTGA CCTGGGTGAG AGCAGAGGTG ACTCAGCGCA TCGCCACCTG CCAGCCCTTC 
TCTACAGCCC TGGACAATGG TCATGTGATC CTGTGTGACA TGATGGCTGA CCCCTGGAAC 
GCCTTCTGGT TTTGCCTGGG GTGGTGCACC TTCTTCCTCA TCCCCAGCAT CATCTTCGCT 
GTCAAGACCT CCAAGTACTT CCGTCCCATC CGGAAACGCC TCAGGGTCAC CTCTCTGAAG 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prom2 ( NM_138750.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_620089.2 Mus musculus prominin 2 (Prom2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10894
Clone ID Related Accession (Same CDS sequence)	NM_138750.2
Accession Version	NM_138750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	prominin-2 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ045574.1, AK137749.1 and AK029851.1. On Mar 14, 2009 this sequence version replaced NM_138750.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029851.1, AK137749.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGCGCT CACCCGGCCT CATGGTGCCC CTGCTGGGCC TGAGCCTGGG ACTGGCCCTG 
AGCCTGCCCG AAGCTGTGAC AGACGACTGC GGGTCCCTCG GGCGCGTGGA GCACCTGGCA 
TTCGCTCGGG TGCCCAGGAC ACGAGAGCTG GCCCCTCTTG TTCGCGCGTC GGGGCCCCTG 
AACTCCCTCT ATGGCACCGT GCGACGATTC CTCTCCGTGG TTCAACTTAA CCCCTTCCCG 
GCAGAGTTGA TAAAGACCCT GCTGAATGAC CCATCTTCTG TGAAGACAGA TGAGGTGGTT 
CGATATGAGG CAGGCTACGT GGTGTGTGCT GTGATTGCTG GCCTCTACCT CCTATTGGTG 
CCCATCACTG GGCTCTGCTT CTGTTGCTGT CGCTGCCGCC AGCGCTGTGG GGGCAGAGTG 
AAGACGGAGC ACAAGGCCAT GGCCTGTGAA CGTGGTACCC TCATGATCTT CCTGCTGCTG 
ACCACCCTCG TGCTTCTGAT TGGTATGGTC TGTGCTTTTG CTACAAACCA ACACACACAT 
AGTCAGACGG GGCCCAGTGT GAAAGCTGTC CCTGAGACCC TGCTCAGCCT AAGAGGCCTG 
GTCTCTGATG TCCCTGAAGA GCTGCGGGCC ATTGCTGAGC AGTTCTCTGT GCCTCAGAAG 
CAAGTCTCAA AGGAGCTGGA TGGTGTTGGT GAGAACCTTG GGAACGTAAT TCATAACCGG 
CTCAAGAGCA CAGTGTACCC AGTGCTAGCC TCAGTGCACA GCCTGGGCCA GGCCCTGCAG 
GTCTCCATAG ACCACCTTCG AGCCCTGAAC ACCACCTCAG TGGAGCTGCA GGAGGCTCAG 
CGGCACCTGG AGCCACCTGT GCAGGCGCAC CGGGAACGCC TGCTCGCCCT GCTTCAGGAC 
AGCTGGTGCC ACGAGGAGAA TTGCAAGCGG GTGCTCAGCC AGGCCGGTGC TCTGCAACTG 
GGCGCTGACT TTAGCCAGAC GCCCCCTGTG GATGATGTCC TGCATCGGCT GAAGGATGTC 
CCAGAAACCA ACTTCTCCAG CATGGTCCAG GAGGAGAAAG CCACCTTCAA TAACCTCCCG 
CTCCTGGTTC AAGTGCAGGC GGTCAGTGTG GTCAAAGATG TGAAAAAGGC ACTGGCTGAG 
CAGCCTGAAG GGTTGAGGAT GCTGGCCCAA GCGTTCCCAG GTTCAGAGGC AGCCTCCCGC 
TGGAGCCAGG CACTGGAGGG GCTGGAACAG CGCAGCCGCC CCTACTTGCA GGAGGTGCAA 
CAATATGAGA CATACAGGTG GATTTTGGGA TGCGTGCTGT GCTCTGCCAT CCTGCTTGTG 
GTGATCTGTA ACCTGCTGGG CCTCAGCCTG GGCATCTGGG GGTTGTTTGC CAGGGAGGAC 
CCCAGCCACT CTGAAACCAA GGGCGAGGCT GGAGCCCGCC TCCTCATGGC AGGTGTGGCC 
TTCAGCTTCC TCTTCGCAGT CCCCCTCATC CTCCTCGTCT TCGTCACTTT CCTGGTGGGT 
GGCAATGTGC AGACGCTGGT GTGCCGGAGC TGGGAGAGTG GAGAGCTGTA TGAGTTTGCA 
GACACTCCGG GGAACTTGCC ACCATCCATG AACCTATCCT ATCTTCTTGG CCTGAAGAAA 
AACATCAGCA TCGTCCAGGC CTATCGACAG TGTAAGGCAG GGGCAGTACT CTGGAAGGTT 
CTACAGCTCA ATGACTCGTA TGACCTGGAT AAGCACCTGG ATATCAAACA GTATACCCAC 
AAGATTCAGC AGGAGCTTCA GAGCTTCCAA GTAGATTTGA AGGAGCTAGA CCTGTTGAGC 
CCCACTGCCC GCCAGGACCT GGAGGCTTTG CAGAGGAGCG GGCTGGAGAA AATCCACTAC 
AGAGGCTTCC TTGTGCAGAT CCAGAAGCCT GTGGTGAACA CTGACATGTG GCAGCTGGCG 
CAGGAGCTGG AAGGACTGGC CCAGGCCCAA AATGACTCTT TACTGAGGCA GCAGCTACGG 
GAGGAGGCCA GAGAACTTCG AAGCCTCTAC CAGGAGAAGG TTGTCCCCCA GGAGAGCCTT 
GTGACCAAGC TCAACTTCAG TGTCAAGACC TTGGAGTCCT TGGCCCCAAG TCTGCAGGTG 
AATACCTCAG ACTTTCTTGA CAGTGTCACT CGTCTAAAAG GAGAGCTGCC TGTGCAGATC 
AACCACATCC TGAGGAATGC CACTGAGTGT TTCCTGACCA GGGAAATGGG CTACTTCTCT 
CAGTATGTGA CCTGGGTGAG AGCAGAGGTG ACTCAGCGCA TCGCCACCTG CCAGCCCTTC 
TCTACAGCCC TGGACAATGG TCATGTGATC CTGTGTGACA TGATGGCTGA CCCCTGGAAC 
GCCTTCTGGT TTTGCCTGGG GTGGTGCACC TTCTTCCTCA TCCCCAGCAT CATCTTCGCT 
GTCAAGACCT CCAAGTACTT CCGTCCCATC CGGAAACGCC TCAGCTCCAC CAGCTCTGAA 
GAGACACAAC TTTTCCATAT CCCCAGGGTC ACCTCTCTGA AGCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620089.2
CDS	98..2605
Translation	MTRSPGLMVPLLGLSLGLALSLPEAVTDDCGSLGRVEHLAFARVPRTRELAPLVRASGPLNSLYGTVRRFLSVVQLNPFPAELIKTLLNDPSSVKTDEVVRYEAGYVVCAVIAGLYLLLVPITGLCFCCCRCRQRCGGRVKTEHKAMACERGTLMIFLLLTTLVLLIGMVCAFATNQHTHSQTGPSVKAVPETLLSLRGLVSDVPEELRAIAEQFSVPQKQVSKELDGVGENLGNVIHNRLKSTVYPVLASVHSLGQALQVSIDHLRALNTTSVELQEAQRHLEPPVQAHRERLLALLQDSWCHEENCKRVLSQAGALQLGADFSQTPPVDDVLHRLKDVPETNFSSMVQEEKATFNNLPLLVQVQAVSVVKDVKKALAEQPEGLRMLAQAFPGSEAASRWSQALEGLEQRSRPYLQEVQQYETYRWILGCVLCSAILLVVICNLLGLSLGIWGLFAREDPSHSETKGEAGARLLMAGVAFSFLFAVPLILLVFVTFLVGGNVQTLVCRSWESGELYEFADTPGNLPPSMNLSYLLGLKKNISIVQAYRQCKAGAVLWKVLQLNDSYDLDKHLDIKQYTHKIQQELQSFQVDLKELDLLSPTARQDLEALQRSGLEKIHYRGFLVQIQKPVVNTDMWQLAQELEGLAQAQNDSLLRQQLREEARELRSLYQEKVVPQESLVTKLNFSVKTLESLAPSLQVNTSDFLDSVTRLKGELPVQINHILRNATECFLTREMGYFSQYVTWVRAEVTQRIATCQPFSTALDNGHVILCDMMADPWNAFWFCLGWCTFFLIPSIIFAVKTSKYFRPIRKRLSSTSSEETQLFHIPRVTSLKL

Target ORF information:

RefSeq Version	NM_138750.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus prominin 2 (Prom2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138750.2

ORF Insert Sequence:

ATGACGCGCT CACCCGGCCT CATGGTGCCC CTGCTGGGCC TGAGCCTGGG ACTGGCCCTG 
AGCCTGCCCG AAGCTGTGAC AGACGACTGC GGGTCCCTCG GGCGCGTGGA GCACCTGGCA 
TTCGCTCGGG TGCCCAGGAC ACGAGAGCTG GCCCCTCTTG TTCGCGCGTC GGGGCCCCTG 
AACTCCCTCT ATGGCACCGT GCGACGATTC CTCTCCGTGG TTCAACTTAA CCCCTTCCCG 
GCAGAGTTGA TAAAGACCCT GCTGAATGAC CCATCTTCTG TGAAGACAGA TGAGGTGGTT 
CGATATGAGG CAGGCTACGT GGTGTGTGCT GTGATTGCTG GCCTCTACCT CCTATTGGTG 
CCCATCACTG GGCTCTGCTT CTGTTGCTGT CGCTGCCGCC AGCGCTGTGG GGGCAGAGTG 
AAGACGGAGC ACAAGGCCAT GGCCTGTGAA CGTGGTACCC TCATGATCTT CCTGCTGCTG 
ACCACCCTCG TGCTTCTGAT TGGTATGGTC TGTGCTTTTG CTACAAACCA ACACACACAT 
AGTCAGACGG GGCCCAGTGT GAAAGCTGTC CCTGAGACCC TGCTCAGCCT AAGAGGCCTG 
GTCTCTGATG TCCCTGAAGA GCTGCGGGCC ATTGCTGAGC AGTTCTCTGT GCCTCAGAAG 
CAAGTCTCAA AGGAGCTGGA TGGTGTTGGT GAGAACCTTG GGAACGTAAT TCATAACCGG 
CTCAAGAGCA CAGTGTACCC AGTGCTAGCC TCAGTGCACA GCCTGGGCCA GGCCCTGCAG 
GTCTCCATAG ACCACCTTCG AGCCCTGAAC ACCACCTCAG TGGAGCTGCA GGAGGCTCAG 
CGGCACCTGG AGCCACCTGT GCAGGCGCAC CGGGAACGCC TGCTCGCCCT GCTTCAGGAC 
AGCTGGTGCC ACGAGGAGAA TTGCAAGCGG GTGCTCAGCC AGGCCGGTGC TCTGCAACTG 
GGCGCTGACT TTAGCCAGAC GCCCCCTGTG GATGATGTCC TGCATCGGCT GAAGGATGTC 
CCAGAAACCA ACTTCTCCAG CATGGTCCAG GAGGAGAAAG CCACCTTCAA TAACCTCCCG 
CTCCTGGTTC AAGTGCAGGC GGTCAGTGTG GTCAAAGATG TGAAAAAGGC ACTGGCTGAG 
CAGCCTGAAG GGTTGAGGAT GCTGGCCCAA GCGTTCCCAG GTTCAGAGGC AGCCTCCCGC 
TGGAGCCAGG CACTGGAGGG GCTGGAACAG CGCAGCCGCC CCTACTTGCA GGAGGTGCAA 
CAATATGAGA CATACAGGTG GATTTTGGGA TGCGTGCTGT GCTCTGCCAT CCTGCTTGTG 
GTGATCTGTA ACCTGCTGGG CCTCAGCCTG GGCATCTGGG GGTTGTTTGC CAGGGAGGAC 
CCCAGCCACT CTGAAACCAA GGGCGAGGCT GGAGCCCGCC TCCTCATGGC AGGTGTGGCC 
TTCAGCTTCC TCTTCGCAGT CCCCCTCATC CTCCTCGTCT TCGTCACTTT CCTGGTGGGT 
GGCAATGTGC AGACGCTGGT GTGCCGGAGC TGGGAGAGTG GAGAGCTGTA TGAGTTTGCA 
GACACTCCGG GGAACTTGCC ACCATCCATG AACCTATCCT ATCTTCTTGG CCTGAAGAAA 
AACATCAGCA TCGTCCAGGC CTATCGACAG TGTAAGGCAG GGGCAGTACT CTGGAAGGTT 
CTACAGCTCA ATGACTCGTA TGACCTGGAT AAGCACCTGG ATATCAAACA GTATACCCAC 
AAGATTCAGC AGGAGCTTCA GAGCTTCCAA GTAGATTTGA AGGAGCTAGA CCTGTTGAGC 
CCCACTGCCC GCCAGGACCT GGAGGCTTTG CAGAGGAGCG GGCTGGAGAA AATCCACTAC 
AGAGGCTTCC TTGTGCAGAT CCAGAAGCCT GTGGTGAACA CTGACATGTG GCAGCTGGCG 
CAGGAGCTGG AAGGACTGGC CCAGGCCCAA AATGACTCTT TACTGAGGCA GCAGCTACGG 
GAGGAGGCCA GAGAACTTCG AAGCCTCTAC CAGGAGAAGG TTGTCCCCCA GGAGAGCCTT 
GTGACCAAGC TCAACTTCAG TGTCAAGACC TTGGAGTCCT TGGCCCCAAG TCTGCAGGTG 
AATACCTCAG ACTTTCTTGA CAGTGTCACT CGTCTAAAAG GAGAGCTGCC TGTGCAGATC 
AACCACATCC TGAGGAATGC CACTGAGTGT TTCCTGACCA GGGAAATGGG CTACTTCTCT 
CAGTATGTGA CCTGGGTGAG AGCAGAGGTG ACTCAGCGCA TCGCCACCTG CCAGCCCTTC 
TCTACAGCCC TGGACAATGG TCATGTGATC CTGTGTGACA TGATGGCTGA CCCCTGGAAC 
GCCTTCTGGT TTTGCCTGGG GTGGTGCACC TTCTTCCTCA TCCCCAGCAT CATCTTCGCT 
GTCAAGACCT CCAAGTACTT CCGTCCCATC CGGAAACGCC TCAGCTCCAC CAGCTCTGAA 
GAGACACAAC TTTTCCATAT CCCCAGGGTC ACCTCTCTGA AGCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

A Grhl2-dependent gene network controls trophoblast branching morphogenesis.
Development (Cambridge, England)142(6)1125-36(2015 Mar)
Walentin K,Hinze C,Werth M,Haase N,Varma S,Morell R,Aue A,P?tschke E,Warburton D,Qiu A,Barasch J,Purf?rst B,Dieterich C,Popova E,Bader M,Dechend R,Staff AC,Yurtdas ZY,Kilic E,Schmidt-Ott KM

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Chromatin regulation by BAF170 controls cerebral cortical size and thickness.
Developmental cell25(3)256-69(2013 May)
Tuoc TC,Boretius S,Sansom SN,Pitulescu ME,Frahm J,Livesey FJ,Stoykova A

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Prom2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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