Ash1l gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006501142.2	XP_006501205.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_017319500.1	XP_017174989.1	histone-lysine N-methyltransferase ASH1L isoform X3
XM_006501141.3	XP_006501204.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_006501142.3	XP_006501205.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_030252468.1	XP_030108328.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_017319502.1	XP_017174991.1	histone-lysine N-methyltransferase ASH1L isoform X4
XM_006501141.2	XP_006501204.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_017319500.2	XP_017174989.1	histone-lysine N-methyltransferase ASH1L isoform X3
NM_138679.5	NP_619620.3	histone-lysine N-methyltransferase ASH1L
XM_017319499.2	XP_017174988.1	histone-lysine N-methyltransferase ASH1L isoform X2
XM_017319499.1	XP_017174988.1	histone-lysine N-methyltransferase ASH1L isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu80570	XM_017319502.1 Latest version!	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu80569	XM_017319500.2 Latest version!	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu80568	XM_017319499.2 Latest version!	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu22302	NM_138679.5 Latest version!	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu22302	XM_006501141.3 Latest version!	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu22302	XM_030252468.1 Latest version!	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu22302	XM_006501142.3 Latest version!	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu22302	XM_006501142.2	Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu22302	XM_006501141.2	Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu80569	XM_017319500.1	Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu80568	XM_017319499.1	Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

Ash1l ( XM_017319502.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174991.1 Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu80570
Clone ID Related Accession (Same CDS sequence)	XM_017319502.1
Accession Version	XM_017319502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT CTGTAGACAG TCTGTTGCAG CGGATAGTAC ATCACGATGA GCAAGAGTCC 
ATGGAGAAAA ACGGTGATGC TTCCATTACA ACTGTGTCTG CACCACCTTC TTCCAGTCCA 
GGCCATAGCT ACAGCAAGGA GCGAGCCTTA GGTAAATCAG ACAGCCTTCT AGTGCCTGCA 
GTCCCGAATG ACTCTTGCAG TAATATCCCA CTTCTTTCTG AAAAGTCAGC AAGTAGGTGT 
TCCCCCCATC ACATCAAGAG AAGTGTAGTT GAAGCTATGC AACGCCAAGC TCGGAAAATG 
TGCAATTATG ACAAAATCTT GGCCACCAAG AAAAACCTGG ACCATGTTAA TAAAATTTTA 
AAAGCCAAAA AACTTCAAAG GCAGGCCAGG ACAGGAAACA ATTTTGTGAA ACGCAGACCA 
GGTCGACCTC GGAAATGCCC CCTCCAAGCA GTGGTGTCAA TGCAAGCCTT CCAGGCCGCC 
CAGTTTGTCA GCCCGGAATT GAATGAAGGC GAAGACATGT CCCTGCACCT AAGTCCAGAC 
ACAGTCACTG ATGTGATCGA GGCTGTGGTT CAGAGTGTGA ACCTCACTTC AGAACATAAG 
AAGGGGGTGA AGAGGAAAAA TTGGCTGTTA GAAGAACAGA CTAGGAAAAA GCAGAAGACA 
GTACCAGAGG AAGAAGAGCA AGAAAACAAT AAAAGCTTTA TAGAAACACC AGTTGAAATT 
CCCAGTCCTC TTGAAACGCC TGCTGAACCT TCTGAACCTG AAAATACCTT GCAACCTGTG 
CTGGCTCTCA TCCCACGAGA AAAGAAGGCC CCACGTCCTC CAAAGAAGAA ATACCAGAGA 
GCTGGGCTGT ATTCTGATGT TTACAAAACA ATAGACCCAA AGAGCCGATT AATCCAATTA 
AAGAAAGAAA AGTTGGAGTA TACTCCAGGA GAGCACGAGT ATGGGTTGTT CCCAGCGCCC 
ATTCATGTTG GAAAGTATCT CAGACAGAAG AGAATTGACT TCCAGCTTCC CTATGATATT 
CTCTGGCAGT GGAAACACAA TCAGCTATAC AAAAAGCCAG ATGTCCCCCT GTATAAAAAA 
ATCCGTTCAA ATGTCTATGT TGATGTCAAA CCACTTTCTG GTTATGAGGC TACCACCTGT 
AATTGTAAGA AGCCAGATGA CGACACCAGG AAGGGCTGTG GGGATGACTG CCTAAATAGA 
ATGATCTTTG CTGAGTGTTC CCCTAACACT TGCCCCTGTG GTGAGCAGTG CTGTAACCAG 
AGGATACAGA GGCATGAGTG GGTGCAGTGT CTGGAACGAT TTCGAGCTGA GGAAAAAGGT 
TGGGGAATTA GAACCAAAGA ACCCCTAAAA GCTGGCCAGT TCATCATTGA ATACCTAGGG 
GAAGTTGTCA GCGAACAAGA ATTCAGGAAC AGGATGATTG AGCAATATCA TAATCACAGT 
GACCACTACT GTTTAAACCT GGATAGCGGA ATGGTGATTG ACAGTTACCG CATGGGAAAT 
GAGGCCAGAT TCATCAACCA CAGCTGTGAC CCAAATTGTG AAATGCAGAA ATGGTCTGTT 
AATGGAGTGT ACCGTATTGG ACTGTATGCA CTTAAGGATA TGCCAGCTGG GACAGAGCTT 
ACCTATGATT ACAACTTTCA TTCCTTCAAT GTAGAAAAAC AGCAACTTTG TAAATGTGGT 
TTTGAGAAGT GTCGAGGAAT CATTGGAGGC AAGAGTCAAC GGATGAACGG ACTCCCTAGC 
CATAAAGGTA GCCAGTCTTC AAGCACGCAT AGAAAGTCTG CACGGGCGAA AGAGAAGCGG 
AAGTCCAAGC ACAAGCTGAA GAAAAGGAAA GGCCATCCCT CTGAAGAGCC CAGTGAAAAC 
ATCAACACCC CAACAAGATT GACTCCACAG TTACAGATGA AGCCAATGTC CAACAGAGAG 
AGGAACTTCG TGCTAAAGCA CCATGTGTTC TTGGTGCGAA ACTGGGAAAA GATCCATCAG 
AAGCAGGAGG AGGTGAAGCA CACCCGTGAC ATCCACTCAG CATCGCTGTA CACCCGCTGG 
AACGGCCTCT GTCGGGACGA CGGGAACATT AAGTCTGATG TCTTCATGAC CCAGTTCTCT 
GCCCTGCAGA CAGCTAGATC TGTTCGAACA AGACGCTTGG CAGCTGCAGA GGAAAACCTG 
GAAGTGGCTC GGGCAGCGCG CCTAGCACAG ATCTTCAAAG AGATCTGTGA TGGCATCATC 
TCCTACAGAG ATTCTTCCCA GCAAACCCTT GCAGCTCCAC TTTTGAATCT TCCCCCAAAG 
AAAAAAAATG CTGACTATTA TGAGAAGATC TCTGACCCCC TGGATCTCAG CACCATAGAG 
AAGCAGATCC TCATTGGGTA TTACAAAACA GTGGAGGCTT TCGATGCTGA CATGCTTAAG 
GTCTTTCGGA ATGCTGAGAA GTACTATGGG CGTAAATCCC CAATTGGGAG AGATGTTTGC 
CGCTTACGAA AGGCCTATTA CAGCGCCCGG CATGAAGCTT CGGCTCAGAT CGATGAGATT 
GTGGGAGAAA CGGCCAGTGA GGCCGACAGC AGCGAGACCT CAGTCTCCGA GAAGGAGAGC 
GGGCACGAGA AGGATGATGA TGTTATCCGC TGCATCTGCG GCCTCTATAA AGATGAAGGC 
CTCATGATCC AGTGTGACAA GTGCATGGTG TGGCAGCACT GCGACTGCAT GGGTGTGAAC 
ACGGATGTGG AGCACTACCT TTGTGAGCAG TGTGACCCTC GGCCTGTGGA CAGGGAGGTA 
CCCATGATCC CTAGGCCCCA CTATGCCCAG CCCGGCTGTG TCTACTTCAT CTGTTTACTC 
CGAGATGACT TGCTGCTTCG TCAGGGTGAT TGTGTGTACC TGATGAGGGA CAGTCGGCGC 
ACGCCTGATG GCCACCCAGT CCGTCAGTCC TACAGACTCT TATCTCACAT TAACCGAGAT 
AAACTTGACA TCTTTAGAAT CGAGAAGCTC TGGAAGAATG AAAAAGAAGA ACGGTTTGCC 
TTTGGTCACC ATTATTTCCG CCCACATGAG ACTCATCACT CTCCATCCCG TCGGTTCTAT 
CACAACGAGC TGTTCCGGGT GCCCCTCTAT GAGATCATTC CCTTGGAGGC CGTAGTTGGG 
ACCTGCTGTG TGTTGGACCT TTATACATAC TGTAAAGGGA GACCCAAAGG AATAAAGGAA 
CAAGATGTGT ATATCTGTGA TTATCGGCTT GACAAGTCAG CACACCTTTT TTACAAGATT 
CATCGGAATC GATATCCGGT CTGCACCAAA CCCTATGCCT TTGATCATTT CCCCAAGAAG 
CTCACTCCCA AAAGAGACTT CTCACCTCAT TATGTCCCAG ACAACTACAA GAGGAACGGA 
GGACGATCAT CCTGGAAGTC TGAACGCTCA AAGCCACCCC TAAAAGACCT AGGACAAGAA 
GATGATGCTT TGCCCTTGAT TGAAGAGGTA CTGGCTAGTC AGGAGCAAGC AGCCAGGGAA 
GTGCCCAGCC CTGAGGAGCC AGACCAGGAA AGAGCCACTG GGGACATTGG GGACGCTGAA 
AAGAAACCAG AGGAGAGCAG TCAAGAAGCC CAGTTAGCCA GCACACCTGA GGAGCGAAGG 
CACAGCCAGA GGGAACGGCT CAACCAGATC CTGCTCAACC TCCTTGAGAA AATACCTGGG 
AAAAATGCCA TTGACGTGAC CTACCTGCTG GAGGAAGGGT CCGGCAGGAA ACTGCGAAGG 
CGCACTTTGT TTATACCAGA AAATAGCTTT CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174991.1
CDS	873..4631
Translation	MDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSEPENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDDTRKGCGDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRMNGLPSHKGSQSSSTHRKSARAKEKRKSKHKLKKRKGHPSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIHQKQEEVKHTRDIHSASLYTRWNGLCRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENLEVARAARLAQIFKEICDGIISYRDSSQQTLAAPLLNLPPKKKNADYYEKISDPLDLSTIEKQILIGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYSARHEASAQIDEIVGETASEADSSETSVSEKESGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGIKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERSKPPLKDLGQEDDALPLIEEVLASQEQAAREVPSPEEPDQERATGDIGDAEKKPEESSQEAQLASTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_017319502.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319502.1

ORF Insert Sequence:

ATGGATGATT CTGTAGACAG TCTGTTGCAG CGGATAGTAC ATCACGATGA GCAAGAGTCC 
ATGGAGAAAA ACGGTGATGC TTCCATTACA ACTGTGTCTG CACCACCTTC TTCCAGTCCA 
GGCCATAGCT ACAGCAAGGA GCGAGCCTTA GGTAAATCAG ACAGCCTTCT AGTGCCTGCA 
GTCCCGAATG ACTCTTGCAG TAATATCCCA CTTCTTTCTG AAAAGTCAGC AAGTAGGTGT 
TCCCCCCATC ACATCAAGAG AAGTGTAGTT GAAGCTATGC AACGCCAAGC TCGGAAAATG 
TGCAATTATG ACAAAATCTT GGCCACCAAG AAAAACCTGG ACCATGTTAA TAAAATTTTA 
AAAGCCAAAA AACTTCAAAG GCAGGCCAGG ACAGGAAACA ATTTTGTGAA ACGCAGACCA 
GGTCGACCTC GGAAATGCCC CCTCCAAGCA GTGGTGTCAA TGCAAGCCTT CCAGGCCGCC 
CAGTTTGTCA GCCCGGAATT GAATGAAGGC GAAGACATGT CCCTGCACCT AAGTCCAGAC 
ACAGTCACTG ATGTGATCGA GGCTGTGGTT CAGAGTGTGA ACCTCACTTC AGAACATAAG 
AAGGGGGTGA AGAGGAAAAA TTGGCTGTTA GAAGAACAGA CTAGGAAAAA GCAGAAGACA 
GTACCAGAGG AAGAAGAGCA AGAAAACAAT AAAAGCTTTA TAGAAACACC AGTTGAAATT 
CCCAGTCCTC TTGAAACGCC TGCTGAACCT TCTGAACCTG AAAATACCTT GCAACCTGTG 
CTGGCTCTCA TCCCACGAGA AAAGAAGGCC CCACGTCCTC CAAAGAAGAA ATACCAGAGA 
GCTGGGCTGT ATTCTGATGT TTACAAAACA ATAGACCCAA AGAGCCGATT AATCCAATTA 
AAGAAAGAAA AGTTGGAGTA TACTCCAGGA GAGCACGAGT ATGGGTTGTT CCCAGCGCCC 
ATTCATGTTG GAAAGTATCT CAGACAGAAG AGAATTGACT TCCAGCTTCC CTATGATATT 
CTCTGGCAGT GGAAACACAA TCAGCTATAC AAAAAGCCAG ATGTCCCCCT GTATAAAAAA 
ATCCGTTCAA ATGTCTATGT TGATGTCAAA CCACTTTCTG GTTATGAGGC TACCACCTGT 
AATTGTAAGA AGCCAGATGA CGACACCAGG AAGGGCTGTG GGGATGACTG CCTAAATAGA 
ATGATCTTTG CTGAGTGTTC CCCTAACACT TGCCCCTGTG GTGAGCAGTG CTGTAACCAG 
AGGATACAGA GGCATGAGTG GGTGCAGTGT CTGGAACGAT TTCGAGCTGA GGAAAAAGGT 
TGGGGAATTA GAACCAAAGA ACCCCTAAAA GCTGGCCAGT TCATCATTGA ATACCTAGGG 
GAAGTTGTCA GCGAACAAGA ATTCAGGAAC AGGATGATTG AGCAATATCA TAATCACAGT 
GACCACTACT GTTTAAACCT GGATAGCGGA ATGGTGATTG ACAGTTACCG CATGGGAAAT 
GAGGCCAGAT TCATCAACCA CAGCTGTGAC CCAAATTGTG AAATGCAGAA ATGGTCTGTT 
AATGGAGTGT ACCGTATTGG ACTGTATGCA CTTAAGGATA TGCCAGCTGG GACAGAGCTT 
ACCTATGATT ACAACTTTCA TTCCTTCAAT GTAGAAAAAC AGCAACTTTG TAAATGTGGT 
TTTGAGAAGT GTCGAGGAAT CATTGGAGGC AAGAGTCAAC GGATGAACGG ACTCCCTAGC 
CATAAAGGTA GCCAGTCTTC AAGCACGCAT AGAAAGTCTG CACGGGCGAA AGAGAAGCGG 
AAGTCCAAGC ACAAGCTGAA GAAAAGGAAA GGCCATCCCT CTGAAGAGCC CAGTGAAAAC 
ATCAACACCC CAACAAGATT GACTCCACAG TTACAGATGA AGCCAATGTC CAACAGAGAG 
AGGAACTTCG TGCTAAAGCA CCATGTGTTC TTGGTGCGAA ACTGGGAAAA GATCCATCAG 
AAGCAGGAGG AGGTGAAGCA CACCCGTGAC ATCCACTCAG CATCGCTGTA CACCCGCTGG 
AACGGCCTCT GTCGGGACGA CGGGAACATT AAGTCTGATG TCTTCATGAC CCAGTTCTCT 
GCCCTGCAGA CAGCTAGATC TGTTCGAACA AGACGCTTGG CAGCTGCAGA GGAAAACCTG 
GAAGTGGCTC GGGCAGCGCG CCTAGCACAG ATCTTCAAAG AGATCTGTGA TGGCATCATC 
TCCTACAGAG ATTCTTCCCA GCAAACCCTT GCAGCTCCAC TTTTGAATCT TCCCCCAAAG 
AAAAAAAATG CTGACTATTA TGAGAAGATC TCTGACCCCC TGGATCTCAG CACCATAGAG 
AAGCAGATCC TCATTGGGTA TTACAAAACA GTGGAGGCTT TCGATGCTGA CATGCTTAAG 
GTCTTTCGGA ATGCTGAGAA GTACTATGGG CGTAAATCCC CAATTGGGAG AGATGTTTGC 
CGCTTACGAA AGGCCTATTA CAGCGCCCGG CATGAAGCTT CGGCTCAGAT CGATGAGATT 
GTGGGAGAAA CGGCCAGTGA GGCCGACAGC AGCGAGACCT CAGTCTCCGA GAAGGAGAGC 
GGGCACGAGA AGGATGATGA TGTTATCCGC TGCATCTGCG GCCTCTATAA AGATGAAGGC 
CTCATGATCC AGTGTGACAA GTGCATGGTG TGGCAGCACT GCGACTGCAT GGGTGTGAAC 
ACGGATGTGG AGCACTACCT TTGTGAGCAG TGTGACCCTC GGCCTGTGGA CAGGGAGGTA 
CCCATGATCC CTAGGCCCCA CTATGCCCAG CCCGGCTGTG TCTACTTCAT CTGTTTACTC 
CGAGATGACT TGCTGCTTCG TCAGGGTGAT TGTGTGTACC TGATGAGGGA CAGTCGGCGC 
ACGCCTGATG GCCACCCAGT CCGTCAGTCC TACAGACTCT TATCTCACAT TAACCGAGAT 
AAACTTGACA TCTTTAGAAT CGAGAAGCTC TGGAAGAATG AAAAAGAAGA ACGGTTTGCC 
TTTGGTCACC ATTATTTCCG CCCACATGAG ACTCATCACT CTCCATCCCG TCGGTTCTAT 
CACAACGAGC TGTTCCGGGT GCCCCTCTAT GAGATCATTC CCTTGGAGGC CGTAGTTGGG 
ACCTGCTGTG TGTTGGACCT TTATACATAC TGTAAAGGGA GACCCAAAGG AATAAAGGAA 
CAAGATGTGT ATATCTGTGA TTATCGGCTT GACAAGTCAG CACACCTTTT TTACAAGATT 
CATCGGAATC GATATCCGGT CTGCACCAAA CCCTATGCCT TTGATCATTT CCCCAAGAAG 
CTCACTCCCA AAAGAGACTT CTCACCTCAT TATGTCCCAG ACAACTACAA GAGGAACGGA 
GGACGATCAT CCTGGAAGTC TGAACGCTCA AAGCCACCCC TAAAAGACCT AGGACAAGAA 
GATGATGCTT TGCCCTTGAT TGAAGAGGTA CTGGCTAGTC AGGAGCAAGC AGCCAGGGAA 
GTGCCCAGCC CTGAGGAGCC AGACCAGGAA AGAGCCACTG GGGACATTGG GGACGCTGAA 
AAGAAACCAG AGGAGAGCAG TCAAGAAGCC CAGTTAGCCA GCACACCTGA GGAGCGAAGG 
CACAGCCAGA GGGAACGGCT CAACCAGATC CTGCTCAACC TCCTTGAGAA AATACCTGGG 
AAAAATGCCA TTGACGTGAC CTACCTGCTG GAGGAAGGGT CCGGCAGGAA ACTGCGAAGG 
CGCACTTTGT TTATACCAGA AAATAGCTTT CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( XM_017319500.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174989.1 Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu80569
Clone ID Related Accession (Same CDS sequence)	XM_017319500.1 , XM_017319500.2
Accession Version	XM_017319500.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGTTTCT 
CAGGAATTGC TGTACAGTGC TGCCAATAAC GATCTCCGGG GATCTGACCC CCCATTCCAC 
CTCCCACTGC CTCTTCAGCA GCTCCAGAGG CCACCAAAAG AAGGAGCTTT GAAGAAAGTG 
CCTGTTCCCT CTTTTACCCT GAGGTGGGTG AGGCCAGGCA GGGTGATTCT CAGGATATCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174989.1
CDS	184..5346
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAVSQELLYSAANNDLRGSDPPFHLPLPLQQLQRPPKEGALKKVPVPSFTLRWVRPGRVILRIS

Target ORF information:

RefSeq Version	XM_017319500.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319500.1

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGTTTCT 
CAGGAATTGC TGTACAGTGC TGCCAATAAC GATCTCCGGG GATCTGACCC CCCATTCCAC 
CTCCCACTGC CTCTTCAGCA GCTCCAGAGG CCACCAAAAG AAGGAGCTTT GAAGAAAGTG 
CCTGTTCCCT CTTTTACCCT GAGGTGGGTG AGGCCAGGCA GGGTGATTCT CAGGATATCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( XM_017319500.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174989.1 Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X5, mRNA.

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CloneID	OMu80569
Clone ID Related Accession (Same CDS sequence)	XM_017319500.1 , XM_017319500.2
Accession Version	XM_017319500.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGTTTCT 
CAGGAATTGC TGTACAGTGC TGCCAATAAC GATCTCCGGG GATCTGACCC CCCATTCCAC 
CTCCCACTGC CTCTTCAGCA GCTCCAGAGG CCACCAAAAG AAGGAGCTTT GAAGAAAGTG 
CCTGTTCCCT CTTTTACCCT GAGGTGGGTG AGGCCAGGCA GGGTGATTCT CAGGATATCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174989.1
CDS	493..5655
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAVSQELLYSAANNDLRGSDPPFHLPLPLQQLQRPPKEGALKKVPVPSFTLRWVRPGRVILRIS

Target ORF information:

RefSeq Version	XM_017319500.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319500.2

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGTTTCT 
CAGGAATTGC TGTACAGTGC TGCCAATAAC GATCTCCGGG GATCTGACCC CCCATTCCAC 
CTCCCACTGC CTCTTCAGCA GCTCCAGAGG CCACCAAAAG AAGGAGCTTT GAAGAAAGTG 
CCTGTTCCCT CTTTTACCCT GAGGTGGGTG AGGCCAGGCA GGGTGATTCT CAGGATATCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( XM_017319499.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174988.1 Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X4, mRNA.

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CloneID	OMu80568
Clone ID Related Accession (Same CDS sequence)	XM_017319499.2 , XM_017319499.1
Accession Version	XM_017319499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGT CTCAGCATCA GGAAGTGTGA GAACCACTGC 
TCTACAGTCT ATAACAGTAG TAATATCTAC TGTATTCGGC CCTGCAAAGA AGTCTACAGT 
ACCTTTTATT GCAACGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174988.1
CDS	493..6693
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAGSQSASDKPSQRSSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASMDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSEPENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGLSIRKCENHCSTVYNSSNIYCIRPCKEVYSTFYCNV

Target ORF information:

RefSeq Version	XM_017319499.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319499.2

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGT CTCAGCATCA GGAAGTGTGA GAACCACTGC 
TCTACAGTCT ATAACAGTAG TAATATCTAC TGTATTCGGC CCTGCAAAGA AGTCTACAGT 
ACCTTTTATT GCAACGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( XM_017319499.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174988.1 Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X3, mRNA.

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CloneID	OMu80568
Clone ID Related Accession (Same CDS sequence)	XM_017319499.2 , XM_017319499.1
Accession Version	XM_017319499.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGT CTCAGCATCA GGAAGTGTGA GAACCACTGC 
TCTACAGTCT ATAACAGTAG TAATATCTAC TGTATTCGGC CCTGCAAAGA AGTCTACAGT 
ACCTTTTATT GCAACGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174988.1
CDS	184..6384
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAGSQSASDKPSQRSSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASMDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSEPENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGLSIRKCENHCSTVYNSSNIYCIRPCKEVYSTFYCNV

Target ORF information:

RefSeq Version	XM_017319499.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319499.1

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGT CTCAGCATCA GGAAGTGTGA GAACCACTGC 
TCTACAGTCT ATAACAGTAG TAATATCTAC TGTATTCGGC CCTGCAAAGA AGTCTACAGT 
ACCTTTTATT GCAACGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( NM_138679.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_619620.3 Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), mRNA.

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CloneID	OMu22302
Clone ID Related Accession (Same CDS sequence)	XM_006501142.2 , XM_006501141.3 , XM_006501142.3 , XM_030252468.1 , XM_006501141.2 , NM_138679.5
Accession Version	NM_138679.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK627544.1, CF167627.1, AK153783.1, BC052194.1, AK033177.1 and AK031724.1. On Sep 22, 2005 this sequence version replaced NM_138679.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619620.3
CDS	101..8977
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAGSQSASDKPSQRSSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASMDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSEPENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDDTRKGCGDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRMNGLPSHKGSQSSSTHRKSARAKEKRKSKHKLKKRKGHPSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIHQKQEEVKHTRDIHSASLYTRWNGLCRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENLEVARAARLAQIFKEICDGIISYRDSSQQTLAAPLLNLPPKKKNADYYEKISDPLDLSTIEKQILIGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYSARHEASAQIDEIVGETASEADSSETSVSEKESGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGIKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERSKPPLKDLGQEDDALPLIEEVLASQEQAAREVPSPEEPDQERATGDIGDAEKKPEESSQEAQLASTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	NM_138679.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138679.5

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( XM_006501142.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501205.1 Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X2, mRNA.

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CloneID	OMu22302
Clone ID Related Accession (Same CDS sequence)	XM_006501142.2 , XM_006501141.3 , XM_006501142.3 , XM_030252468.1 , XM_006501141.2 , NM_138679.5
Accession Version	XM_006501142.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501205.1
CDS	176..9052
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAGSQSASDKPSQRSSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASMDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSEPENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDDTRKGCGDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRMNGLPSHKGSQSSSTHRKSARAKEKRKSKHKLKKRKGHPSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIHQKQEEVKHTRDIHSASLYTRWNGLCRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENLEVARAARLAQIFKEICDGIISYRDSSQQTLAAPLLNLPPKKKNADYYEKISDPLDLSTIEKQILIGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYSARHEASAQIDEIVGETASEADSSETSVSEKESGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGIKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERSKPPLKDLGQEDDALPLIEEVLASQEQAAREVPSPEEPDQERATGDIGDAEKKPEESSQEAQLASTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_006501142.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501142.2

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( XM_006501141.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501204.1 Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X1, mRNA.

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CloneID	OMu22302
Clone ID Related Accession (Same CDS sequence)	XM_006501142.2 , XM_006501141.3 , XM_006501142.3 , XM_030252468.1 , XM_006501141.2 , NM_138679.5
Accession Version	XM_006501141.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501204.1
CDS	493..9369
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAGSQSASDKPSQRSSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASMDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSEPENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDDTRKGCGDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRMNGLPSHKGSQSSSTHRKSARAKEKRKSKHKLKKRKGHPSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIHQKQEEVKHTRDIHSASLYTRWNGLCRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENLEVARAARLAQIFKEICDGIISYRDSSQQTLAAPLLNLPPKKKNADYYEKISDPLDLSTIEKQILIGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYSARHEASAQIDEIVGETASEADSSETSVSEKESGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGIKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERSKPPLKDLGQEDDALPLIEEVLASQEQAAREVPSPEEPDQERATGDIGDAEKKPEESSQEAQLASTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_006501141.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501141.3

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( XM_006501142.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501205.1 Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X2, mRNA.

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CloneID	OMu22302
Clone ID Related Accession (Same CDS sequence)	XM_006501142.2 , XM_006501141.3 , XM_006501142.3 , XM_030252468.1 , XM_006501141.2 , NM_138679.5
Accession Version	XM_006501142.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501205.1
CDS	254..9130
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAGSQSASDKPSQRSSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASMDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSEPENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDDTRKGCGDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRMNGLPSHKGSQSSSTHRKSARAKEKRKSKHKLKKRKGHPSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIHQKQEEVKHTRDIHSASLYTRWNGLCRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENLEVARAARLAQIFKEICDGIISYRDSSQQTLAAPLLNLPPKKKNADYYEKISDPLDLSTIEKQILIGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYSARHEASAQIDEIVGETASEADSSETSVSEKESGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGIKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERSKPPLKDLGQEDDALPLIEEVLASQEQAAREVPSPEEPDQERATGDIGDAEKKPEESSQEAQLASTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_006501142.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501142.3

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( XM_030252468.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108328.1 Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X3, mRNA.

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CloneID	OMu22302
Clone ID Related Accession (Same CDS sequence)	XM_006501142.2 , XM_006501141.3 , XM_006501142.3 , XM_030252468.1 , XM_006501141.2 , NM_138679.5
Accession Version	XM_030252468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108328.1
CDS	320..9196
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAGSQSASDKPSQRSSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASMDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSEPENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDDTRKGCGDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRMNGLPSHKGSQSSSTHRKSARAKEKRKSKHKLKKRKGHPSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIHQKQEEVKHTRDIHSASLYTRWNGLCRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENLEVARAARLAQIFKEICDGIISYRDSSQQTLAAPLLNLPPKKKNADYYEKISDPLDLSTIEKQILIGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYSARHEASAQIDEIVGETASEADSSETSVSEKESGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGIKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERSKPPLKDLGQEDDALPLIEEVLASQEQAAREVPSPEEPDQERATGDIGDAEKKPEESSQEAQLASTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_030252468.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ASH1 like histone lysine methyltransferase (Ash1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252468.1

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ash1l ( XM_006501141.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501204.1 Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X1, mRNA.

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CloneID	OMu22302
Clone ID Related Accession (Same CDS sequence)	XM_006501142.2 , XM_006501141.3 , XM_006501142.3 , XM_030252468.1 , XM_006501141.2 , NM_138679.5
Accession Version	XM_006501141.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501204.1
CDS	184..9060
Translation	MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATEEEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEESIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKLAQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALSTAVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSKESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVASSAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQELPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSSERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPVTRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIECKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLKLGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSELFKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPSHKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVATESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILPPLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQPQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQWVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGSCRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAGSQSASDKPSQRSSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASMDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSEPENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDDTRKGCGDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRMNGLPSHKGSQSSSTHRKSARAKEKRKSKHKLKKRKGHPSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIHQKQEEVKHTRDIHSASLYTRWNGLCRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENLEVARAARLAQIFKEICDGIISYRDSSQQTLAAPLLNLPPKKKNADYYEKISDPLDLSTIEKQILIGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYSARHEASAQIDEIVGETASEADSSETSVSEKESGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGIKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERSKPPLKDLGQEDDALPLIEEVLASQEQAAREVPSPEEPDQERATGDIGDAEKKPEESSQEAQLASTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_006501141.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ash1 (absent, small, or homeotic)-like (Drosophila) (Ash1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501141.2

ORF Insert Sequence:

ATGGACCCTA GGAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
AAGAGTCCTT CTACCATCAA TCCTGGCACT CTGGCCAGCA AAAGAGAAGC AGAGATAGAG 
GGTGCCACAG AAGAAGAGGA TCCTCGCAAG CGGAATCGAG AAAGGGGCAC AGAAGCAGGA 
AAGGAGGATG GCTCGACGGA TGCACAGCAA CAGTTTTCAG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAATTGAA AATTGGCCTT CAGGCAAAGA GAACTAAAAA GCCTCCAAAG 
AACTTGGAGA ACTATGTGTG TCGACCTGCC ATAAAGACAA CCATCAAGCA CTCAAGGAAA 
GCACTTAAAA GTGGGAAGAT GACAGATGAG AAGAATGAGC ACTGCCCTTC AAAATGGGAC 
TCTTCAAAAT TGTTCAAGAA AGCAGGTGAC GCTACAGCCA TCGACTGCCA AGCCGAAGAA 
AGCATCCATC TTCATTCACA GGGGGAAAGT AATCCTTTGT CTAAGAAGCT TTCTCCAGTA 
CATTCACAAA TGGCAGATTA CATTAGTGCA GCACCATCTC TTGTTGGCAG TCGAGATCCT 
GATATAAAGG ACAGAGCATT ACTTAATGGA GGAACTAGTG TAACAGAAAA GTTGGCACAG 
CTCATTGCTA CCTGTCCTCC TTCCAAGTCT TCCAAGGCAA AACCGAAGAA GTTAGGAACT 
GGCACTACAG TTGGATTGGT TAGCAAGGAC TTGATCAGGA AACCAGGTGT TGGCTCTATA 
GCTGGAATAA TTCATAAAGA CTTAATTAAA AAGCCAGCTC TTAGCACAGC AGTTGGGTTG 
GTAACTAAAG ATCCAGGGAA AAAGCCTATG TTCAATGCAG CAGTAGGCCT GATCAATAAG 
GACTCTGTGA AAAAATTGGG AACTGGCACT ACAGCCGTAT TCATTAATAA AGACTTAGGC 
AAAAAGCCAG GGGCTATCAC TACAGTTGGA CTGCTGAGCA AAGAATCAGG AAAGAAGCTA 
GGCATTGGTA TTGTTCCAGG GCTAGTGAAT AAAGAGTCTG GCAAGAAATT AGGACTTGGC 
ACCGTAGTTG GACTGGTTAA TAAAGAGTTG GGAAAGAAAC TGAGTTCTAC AGTTGGCCTA 
GTGGCCAAGG ATGTTACGAA GAAGATTGTA GCAAGCTCAG CAATGGGATT GGTTAATAAG 
GACATTGGAA AGAAATTATT AAATTGTCCT ATGGCAGGAC AACTGGGCAG TAAAGATGCC 
TTAAACCTTA AATCTGAAGC ACTGCTCCCC ACTCAGGAAC AACTTAAGGC TTCCTGTAGT 
GCAAACATAA GTAATCATGA CAGTCAAGAA CTTCCTGAGT CTCTGAAAGA CAGTGCTACC 
GGCAAAGCTT TTGAGAAGAG TGTTATGCGG CATAGTAAAG AAAGCATGTT GGAAAAATTT 
TCAGTCCGAA AAGAAATTAC TAATTTGGAG AAAGAAATGT TTAATGAAGG AACATGCATT 
CAGCAAGACA ATTTCTCATC TAGTGAAAGG GGAGCCTTTG AAACCTCAAA ACATGAAAAG 
CAGCCTCCTG TGTATTGCAC TTCTCCTGAC TTTCAAATAG GAGGTGCCTC TGATGCCTCT 
ACAGCTAAAT CTCCCTTCAG TGCAGTAGGC GAAAGCAACC TTCCTTCCTC GTCACCTACT 
GTATCTGTTA ATCCTGTAAC CAGGAGTCCC CCTGAAGCTT CTTCACAGTT GGTTCCAAAC 
CCATTACTTT TAAATTCTAC AGCAGAACAA ATGGAAGAAA TCTCTGAATC TATTGGAAAG 
AGCCAGTTTA CTGCTGAAAG TACCCACTTG AATGTTGGTC ACAGATCATT AGGTCATAGC 
TTAAGTATTG AGTGTAAAGG AATTGATAAA GAACTAAATG AATCAAAAAA TACACATCTA 
GATATTCCTA GAATAAGTTC TTCCTTGGGA AAAAAGCCAA GTTTGACTTC TGACTCTGGT 
ATTCATGCAA TTACTCCTTC CGTTGTTAAT TTTACTAGTT TGTTTAGTAA CAAACCTTTT 
TTAAAGCTTG GTGCAGTCAC TGCACCAGAC AAACACTGCC AGGTTGCTGA AAGCCTAAGC 
TCGAGTTTCC AGTCCAAACC ATTGAAAAAA AGAAAAGGAA GAAAACCTCG GTGGACTAAA 
GTGGTGGCAA GAAGCACATG CCGGTCTCCA AAAGGACTAG ACTTAGAAAG ATCAGAGCTT 
TTTAAGAATG TTTCTTGTAG CTCACTATCA AATAGTTCTG AGCCAGCGAA GTTTATGAAA 
ACTATCGGAG CATCATCATT CGTCGATCAT GACTTCCTTA AACGCCGATT GCCAAAGTTG 
AGTAAGTCTT CAGCTCCATC TCTTGCTCTT TTAACCGACA GCGAGAAACC ATCTCATAAG 
TCTTTTATTA CTCACAAACT GTCCTCCAGT ATGTGTGTCA CTAGCGATCT TTTGTCGGAT 
ATTTATAAGC CCAAGAGAGG AAGACCTAAA TCCAAGGAAA TGCCTCAGCT AGAAGGTCCA 
CCTAAAAGGA CTTTAAAAAT ACCTGCCTCT AAAGTTTTTT CTTTACAGTC TAAAGAAGAG 
CAAGAACCCC CAATTTTACA ACCAGAAATT GAAATTCCTT CCTTCAAGCA AAGTTTGTCT 
GTATCTCCTT TTCCAAAAAA AAGAGGCAGG CCTAAGAGGC AGATGAGGTC TCCAGTGAAG 
ATGAAACCCC CTGTCCTGTC AGTGGCTCCA TTTGTTGCCA CTGAAAGTCC AAGTAAGCTC 
GAGTCTGAAA GTGAGAACCA TAGAAGTAGC AGTGACTTTT TTGAGAGTGA GGACCAGCTT 
CAGGACACAG ACGACTTAGA TGACAGTCAC AGGCAAAGTG TCTGTAGTAT GAGTGACCTT 
GAGATGGAAC CAGATAAAAA AATTAGCAAG CGAAACAATG GACAATTAAT GAAAACAATT 
ATCCGAAAAA TAAATAAAAT GAAGACTTTA AAGAGAAAAA AACTGTTGAA TCAGATTCTT 
TCGAGTTCTG TAGAATCAAG TAATAAAGGA AAAGTGCAGT CCAAGCTTCA CAATACAGTC 
TCAAGTCTTG CTGCCACATT TGGCTCTAAA TTGGGGCAAC AAATCAATGT CAGCAAGAAA 
GGAACCATTT ACATAGGGAA GAGAAGGGGG CGGAAACCAA AAACCGTCTT AAATGGCCTG 
CTTTCTGGTA GCCCTGCCAG CCTTGCTGTG CTTGAACAAA CAGCTCAGCA GGCAGCTGGG 
TCAGCATTAG GACAGATCCT CCCCCCTTTA CTGCCATCAC CTGCTAGTAG CTCTGAGATC 
CTTCCATCAC CTATTTGCTC TCAGTCTTCT GGGACTAGTG GAGGTCAGAG CCCTGTTAGT 
AGTGACGCAG GCTTTGTTGA ACCTAGCTCA GTGCCATATT TGCATGTGCA CTCCAGACAG 
GGCAGTATGA TTCAGACTCT TGCAATGAAG AAGGCTTCCA AGGGCAGAAG GCGGCTCTCT 
CCTCCTACTT TGTTGCCAAA TTCTCCTTCT CATTTGAGTG AACTCACATC CCTGAAAGAA 
GCCACTCCTT CCCCAGTTAG TGAGTCTCAT AGTGACGAGA CCATTCCCAG TGACAGTGGC 
ATTGGGACAG ATAATAATAG CACATCAGAC AGGGCAGAGA AGTTTTGTGG ACAGAAAAAG 
AGGAGACATT CTTTTGAGCA CATTTCTCTG ATTCCCCCTG AAACTTCTAC CGTCCTAAAC 
AGTCTCAAAG AAAAACACAA ACACAAATGT AAGCGCAGGA GTCACGATTA CCTCAGCTAT 
GACAAGATGA AAAGACAGAA GCGAAAACGG AAAAAGAAAT ATCCCCAGCT TCGAAATAGG 
CAGGATCCAG ACTTCATCGC AGAACTAGAG GAGCTGATAA GTCGTCTAAG TGAGATTCGG 
ATCACCCATC GAAGCCATCA TTTTATTCCC CGAGACCTCC TGCCAACTAT TTTTCGAATC 
AACTTTAATA GTTTCTATAC ACATCCTTCT TTTCCCTTAG ACCCATTGCA CTACATTCGG 
AAACCTGACT TAAAAAAGAA ACGAGGGAGG CCCCCTAAGA TGAGGGAGGC AATGGCAGAA 
ATGCCTTTTA TGCACAGCCT TAGTTTTCCT TTATCTAGTA CTGGATTTTA TCCTTCTTAT 
GGCATGCCTT ACTCTCCCTC ACCCCTTACA GCTGCTCCAA TAGGATTAGG GTATTATGGA 
AGGTATCCCC CCACTCTTTA TCCACCGCCA CCATCTCCTT CATTCACCAC TCCACTGCCA 
CCTCCCTCCT ATATGCATGC TGGTCATTTA TTGCTCAATC CTACCAAATA TCATAAGAAA 
AAGCATAAGC TGCTTAGACA GGAGGCTTTT CTTACAACCA GCAGGACCCC TCTCCTTTCC 
ATGAGCACCT ACCCCAGCGT ACCTCCAGAG ATGGCTTATG GTTGGATGGT GGAGCACAAA 
CACAGACACC GTCACAAACA CAGAGAGCAC CGTTCTGAAC AGCCCCAGGT TTCCATGGAT 
AGTGGCTCTT CCAGATCCGT CTTGGAGTCT TTGAAACGTT ACCGATTTGG TAAGGATACT 
GTTGGAGATC GATATAAGCA TAAGGAAAAG CACAGGTGTC ACATGTCCTG CCCTCATCTC 
TCTCCTTCAA AGAACTTAAT AAACAGAGAA GAACAGTGGG TTTCTCGAGA GCCTTCAGAA 
TCAAGTTCTT TAGCCTTGGG ATTGCAAACA CCTTTACAAA TTGACTGCTC AGAAAGTTCT 
CCCAGTTTGT CCCTTGGAGG ATTTACTCCC AATTCTGAGC CAGCCAGCAG TGATGAACAT 
ATGAACCTTT TTACAAGTGC AATAGGCAGC TGCAGAGTTT CAAACCCTAA CTCCAGTTGC 
CGGAAGAAAT TAACTGACAG CCCTGGGCTC TTTCCTGTAC AAGATACTGC ACTAAATCGG 
CCTCACAGAA AGGAACCATT ACCTTCCAGT GAAAGGGCAA TACAGTCGTT GGCAGGTTCC 
CAGTCAGCTT CCGATAAACC TTCCCAGCGA TCATCAGAAA GCACAAATTG TAGCCCTACT 
CGTAAAAGGT CTTCATCTGA GAGTACTTCT TCAACAGTGA ATGGAGTTCC TTCCAGAAGT 
CCCAGATTGG TTGCTTCTAT GGATGATTCT GTAGACAGTC TGTTGCAGCG GATAGTACAT 
CACGATGAGC AAGAGTCCAT GGAGAAAAAC GGTGATGCTT CCATTACAAC TGTGTCTGCA 
CCACCTTCTT CCAGTCCAGG CCATAGCTAC AGCAAGGAGC GAGCCTTAGG TAAATCAGAC 
AGCCTTCTAG TGCCTGCAGT CCCGAATGAC TCTTGCAGTA ATATCCCACT TCTTTCTGAA 
AAGTCAGCAA GTAGGTGTTC CCCCCATCAC ATCAAGAGAA GTGTAGTTGA AGCTATGCAA 
CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC 
CATGTTAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGGC AGGCCAGGAC AGGAAACAAT 
TTTGTGAAAC GCAGACCAGG TCGACCTCGG AAATGCCCCC TCCAAGCAGT GGTGTCAATG 
CAAGCCTTCC AGGCCGCCCA GTTTGTCAGC CCGGAATTGA ATGAAGGCGA AGACATGTCC 
CTGCACCTAA GTCCAGACAC AGTCACTGAT GTGATCGAGG CTGTGGTTCA GAGTGTGAAC 
CTCACTTCAG AACATAAGAA GGGGGTGAAG AGGAAAAATT GGCTGTTAGA AGAACAGACT 
AGGAAAAAGC AGAAGACAGT ACCAGAGGAA GAAGAGCAAG AAAACAATAA AAGCTTTATA 
GAAACACCAG TTGAAATTCC CAGTCCTCTT GAAACGCCTG CTGAACCTTC TGAACCTGAA 
AATACCTTGC AACCTGTGCT GGCTCTCATC CCACGAGAAA AGAAGGCCCC ACGTCCTCCA 
AAGAAGAAAT ACCAGAGAGC TGGGCTGTAT TCTGATGTTT ACAAAACAAT AGACCCAAAG 
AGCCGATTAA TCCAATTAAA GAAAGAAAAG TTGGAGTATA CTCCAGGAGA GCACGAGTAT 
GGGTTGTTCC CAGCGCCCAT TCATGTTGGA AAGTATCTCA GACAGAAGAG AATTGACTTC 
CAGCTTCCCT ATGATATTCT CTGGCAGTGG AAACACAATC AGCTATACAA AAAGCCAGAT 
GTCCCCCTGT ATAAAAAAAT CCGTTCAAAT GTCTATGTTG ATGTCAAACC ACTTTCTGGT 
TATGAGGCTA CCACCTGTAA TTGTAAGAAG CCAGATGACG ACACCAGGAA GGGCTGTGGG 
GATGACTGCC TAAATAGAAT GATCTTTGCT GAGTGTTCCC CTAACACTTG CCCCTGTGGT 
GAGCAGTGCT GTAACCAGAG GATACAGAGG CATGAGTGGG TGCAGTGTCT GGAACGATTT 
CGAGCTGAGG AAAAAGGTTG GGGAATTAGA ACCAAAGAAC CCCTAAAAGC TGGCCAGTTC 
ATCATTGAAT ACCTAGGGGA AGTTGTCAGC GAACAAGAAT TCAGGAACAG GATGATTGAG 
CAATATCATA ATCACAGTGA CCACTACTGT TTAAACCTGG ATAGCGGAAT GGTGATTGAC 
AGTTACCGCA TGGGAAATGA GGCCAGATTC ATCAACCACA GCTGTGACCC AAATTGTGAA 
ATGCAGAAAT GGTCTGTTAA TGGAGTGTAC CGTATTGGAC TGTATGCACT TAAGGATATG 
CCAGCTGGGA CAGAGCTTAC CTATGATTAC AACTTTCATT CCTTCAATGT AGAAAAACAG 
CAACTTTGTA AATGTGGTTT TGAGAAGTGT CGAGGAATCA TTGGAGGCAA GAGTCAACGG 
ATGAACGGAC TCCCTAGCCA TAAAGGTAGC CAGTCTTCAA GCACGCATAG AAAGTCTGCA 
CGGGCGAAAG AGAAGCGGAA GTCCAAGCAC AAGCTGAAGA AAAGGAAAGG CCATCCCTCT 
GAAGAGCCCA GTGAAAACAT CAACACCCCA ACAAGATTGA CTCCACAGTT ACAGATGAAG 
CCAATGTCCA ACAGAGAGAG GAACTTCGTG CTAAAGCACC ATGTGTTCTT GGTGCGAAAC 
TGGGAAAAGA TCCATCAGAA GCAGGAGGAG GTGAAGCACA CCCGTGACAT CCACTCAGCA 
TCGCTGTACA CCCGCTGGAA CGGCCTCTGT CGGGACGACG GGAACATTAA GTCTGATGTC 
TTCATGACCC AGTTCTCTGC CCTGCAGACA GCTAGATCTG TTCGAACAAG ACGCTTGGCA 
GCTGCAGAGG AAAACCTGGA AGTGGCTCGG GCAGCGCGCC TAGCACAGAT CTTCAAAGAG 
ATCTGTGATG GCATCATCTC CTACAGAGAT TCTTCCCAGC AAACCCTTGC AGCTCCACTT 
TTGAATCTTC CCCCAAAGAA AAAAAATGCT GACTATTATG AGAAGATCTC TGACCCCCTG 
GATCTCAGCA CCATAGAGAA GCAGATCCTC ATTGGGTATT ACAAAACAGT GGAGGCTTTC 
GATGCTGACA TGCTTAAGGT CTTTCGGAAT GCTGAGAAGT ACTATGGGCG TAAATCCCCA 
ATTGGGAGAG ATGTTTGCCG CTTACGAAAG GCCTATTACA GCGCCCGGCA TGAAGCTTCG 
GCTCAGATCG ATGAGATTGT GGGAGAAACG GCCAGTGAGG CCGACAGCAG CGAGACCTCA 
GTCTCCGAGA AGGAGAGCGG GCACGAGAAG GATGATGATG TTATCCGCTG CATCTGCGGC 
CTCTATAAAG ATGAAGGCCT CATGATCCAG TGTGACAAGT GCATGGTGTG GCAGCACTGC 
GACTGCATGG GTGTGAACAC GGATGTGGAG CACTACCTTT GTGAGCAGTG TGACCCTCGG 
CCTGTGGACA GGGAGGTACC CATGATCCCT AGGCCCCACT ATGCCCAGCC CGGCTGTGTC 
TACTTCATCT GTTTACTCCG AGATGACTTG CTGCTTCGTC AGGGTGATTG TGTGTACCTG 
ATGAGGGACA GTCGGCGCAC GCCTGATGGC CACCCAGTCC GTCAGTCCTA CAGACTCTTA 
TCTCACATTA ACCGAGATAA ACTTGACATC TTTAGAATCG AGAAGCTCTG GAAGAATGAA 
AAAGAAGAAC GGTTTGCCTT TGGTCACCAT TATTTCCGCC CACATGAGAC TCATCACTCT 
CCATCCCGTC GGTTCTATCA CAACGAGCTG TTCCGGGTGC CCCTCTATGA GATCATTCCC 
TTGGAGGCCG TAGTTGGGAC CTGCTGTGTG TTGGACCTTT ATACATACTG TAAAGGGAGA 
CCCAAAGGAA TAAAGGAACA AGATGTGTAT ATCTGTGATT ATCGGCTTGA CAAGTCAGCA 
CACCTTTTTT ACAAGATTCA TCGGAATCGA TATCCGGTCT GCACCAAACC CTATGCCTTT 
GATCATTTCC CCAAGAAGCT CACTCCCAAA AGAGACTTCT CACCTCATTA TGTCCCAGAC 
AACTACAAGA GGAACGGAGG ACGATCATCC TGGAAGTCTG AACGCTCAAA GCCACCCCTA 
AAAGACCTAG GACAAGAAGA TGATGCTTTG CCCTTGATTG AAGAGGTACT GGCTAGTCAG 
GAGCAAGCAG CCAGGGAAGT GCCCAGCCCT GAGGAGCCAG ACCAGGAAAG AGCCACTGGG 
GACATTGGGG ACGCTGAAAA GAAACCAGAG GAGAGCAGTC AAGAAGCCCA GTTAGCCAGC 
ACACCTGAGG AGCGAAGGCA CAGCCAGAGG GAACGGCTCA ACCAGATCCT GCTCAACCTC 
CTTGAGAAAA TACCTGGGAA AAATGCCATT GACGTGACCT ACCTGCTGGA GGAAGGGTCC 
GGCAGGAAAC TGCGAAGGCG CACTTTGTTT ATACCAGAAA ATAGCTTTCG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Ash1l
Entrez Gene ID	192195
Full Name	ASH1 like histone lysine methyltransferase
Synonyms	8030453L17Rik,Ash1,E430018P19Rik,Kmt2h
General protein information	Preferred Names ASH1 like histone lysine methyltransferase Names histone-lysine N-methyltransferase ASH1L ASH1-like protein absent small and homeotic disks protein 1 homolog absent, small, or homeotic discs 1 ash1 (absent, small, or homeotic)-like chromatin remodeling factor probable histone-lysine N-methyltransferase ASH1L
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F1

Pan troglodytes (chimpanzee)	ASH1L	XP_003949641.1
Bos taurus (cattle)	ASH1L	NP_001179672.1
Canis lupus familiaris (dog)	ASH1L	XP_537251.2
Rattus norvegicus (Norway rat)	Ash1l	NP_001101159.1
Mus musculus (house mouse)	Ash1l	NP_619620.3
Gallus gallus (chicken)	ASH1L	XP_004948339.1
Homo sapiens (human)	ASH1L	NP_060959.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Ash1l cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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